Abstracts of the 2nd MC-GARD (Marie Curie-Genome Architecture in Relation to Disease) Meeting. May 4-7, 2008. Madrid, Spain.
Authors:
PMID: 18574904 [PubMed - indexed for MEDLINE] (Source: Analytical Cellular Pathology)
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
Molecular detection of neuron-specific ELAV-like-positive cells in the peripheral blood of patients with small-cell lung cancer.
CONCLUSION: Our preliminary results suggest that neuron-ELAV mRNA are detectable in peripheral blood of SCLC patients using real-time quantitative PCR.
PMID: 18607064 [PubMed - indexed for MEDLINE] (Source: Analytical Cellular Pathology)
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
Hypermethylation of the FANCC and FANCL promoter regions in sporadic acute leukaemia.
CONCLUSION: Hypermethylation of promoter regions from FA-BRCA genes does occur in sporadic leukaemia, albeit infrequently. Hypermethylation was found to result in hypersensitivity towards DNA cross-linking agents, a hallmark of the FA cellular phenotype, suggesting that these samples displayed chromosomal instability. This instability may have contributed to the occurrence of the leukaemia. In addition, this is the first report to describe hypermethylation of FANCC and FANCL. This warrants the investigation of multiple FA-BRCA genes in other malignancies.
PMID: 18607065 [PubMed - indexed for MEDLINE] (Source: Analy...
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
Mitochondrial DNA depletion reduces PARP-1 levels and promotes progression of the neoplastic phenotype in prostate carcinoma.
Authors: Moro L, Arbini AA, Marra E, Greco M
Abstract
Mitochondrial dysfunction resulting from mitochondrial DNA (mtDNA) mutations and/or depletion has been correlated with cancer progression and drug resistance. To investigate the role of mtDNA in prostate cancer progression, we used LNCaP and PC-3 prostate carcinoma cells as experimental model. Compared to minimally invasive androgen-dependent LNCaP cells, highly invasive androgen-independent PC-3 cells, as well as androgen-independent DU145 and C4-2 cells, exhibited significantly reduced mtDNA content. In PC-3 cells, reduction of mtDNA was accompanied b...
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
MLPAnalyzer: data analysis tool for reliable automated normalization of MLPA fragment data.
DISCUSSION: Visual examination of bar graphs and direct ratios for both techniques showed very similar results, while the average Pearson moment correlation over all MLPA probes was found to be 0.42. Our results thus show that automated MLPA data processing following our suggested strategy may be of significant use, especially when handling large MLPA data sets, when samples are of different quality, or interpretation of MLPA electropherograms is too complex. It remains, however, important to recognize that automated MLPA data processing may only be successful when a dedicated experimental setup is also considered.
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Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
Altered cellular distribution and sub-cellular sorting of doppel (Dpl) protein in human astrocytoma cell lines.
In this study we have analysed the sub-cellular localization of Dpl using double-immunofluorescence staining and confocal microscopy examinations in two astrocytoma-derived human cell lines (IPDDC-A2 and D384-MG). Our results confirmed that Dpl is localized in the cytoplasm of the astrocytoma cells and indicated that it is mostly associated with Lamp-1 and Limp-2 positive lysosomal vesicles and, marginally, to the Golgi apparatus and other cellular organelles. Noticeably, none of the examined tumor cells showed a membrane-Dpl localization. The membrane-associated Dpl expression was restored after the transfection of the as...
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
Estrogen receptor beta displays cell cycle-dependent expression and regulates the G1 phase through a non-genomic mechanism in prostate carcinoma cells.
CONCLUSIONS: Our results demonstrate that, in LNCaP prostate cancer cells, both ERbeta isoforms are differentially expressed during the cell cycle and that ERbeta regulates the G1 phase by a non-genomic mechanism.
PMID: 18607069 [PubMed - indexed for MEDLINE] (Source: Analytical Cellular Pathology)
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
PALGA, the nationwide histo- and cytopathology data network and archive. A role for digital pathology?
Authors:
PMID: 18683361 [PubMed - indexed for MEDLINE] (Source: Analytical Cellular Pathology)
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
Tyrosinase overexpression promotes ATM-dependent p53 phosphorylation by quercetin and sensitizes melanoma cells to dacarbazine.
In conclusion, these results suggest that Qct or Qct analogues may significantly improve DTIC response rates in tumors that express tyrosinase.
PMID: 18791269 [PubMed - indexed for MEDLINE] (Source: Analytical Cellular Pathology)
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
Overexpression of the mitotic checkpoint genes BUB1 and BUBR1 is associated with genomic complexity in clear cell kidney carcinomas.
CONCLUSIONS: We conclude that BUB1 and BUBR1 overexpression plays a role in cytogenetic and morphologic progression of ccRCC.
PMID: 18791270 [PubMed - indexed for MEDLINE] (Source: Analytical Cellular Pathology)
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
Conditional inactivation of HIF-1 using intrabodies.
Authors: Groot AJ, Gort EH, van der Wall E, van Diest PJ, Vooijs M
Abstract
Hypoxia is a hallmark of solid cancers and triggers the transcription of genes responsible for cell survival. The transcription factor Hypoxia-Inducible Factor 1 (HIF-1) is a key regulator in this response and frequently activated in human cancer. HIF-1 activation is associated with tumor aggressiveness and poor clinical outcome and, therefore, may provide an attractive therapeutic target. Here we provide a novel approach for HIF-1 targeted therapy using single-domain llama antibodies directed against the HIF-1alpha oxygen dependen...
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
PRIMA-1MET inhibits growth of mouse tumors carrying mutant p53.
Authors: Zache N, Lambert JM, Wiman KG, Bykov VJ
Abstract
Reactivation of the tumor suppressor activity to mutant p53 should trigger massive apoptosis and eliminate tumors. The low molecular weight compounds PRIMA-1 and the structural analog PRIMA-1MET reactivate human mutant p53 in vitro and suppress growth of human tumor xenografts in SCID mice. However, little is known about their effect on mouse mutant p53 in mouse tumor cells. We have examined the effect of PRIMA-1MET on mouse sarcomas, mammary carcinomas and chemically induced fibrosarcomas. PRIMA-1MET showed potent growth suppression in mutant p53-c...
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
HA14-1 is able to reconstitute the impaired mitochondrial pathway of apoptosis in renal cell carcinoma cell lines.
Authors: Heikaus S, van den Berg L, Kempf T, Mahotka C, Gabbert HE, Ramp U
Abstract
Renal cell carcinomas (RCCs) exhibit a marked resistance towards apoptosis. Although most apoptotic stimuli converge at the level of the mitochondria, little is known about the mitochondrial apoptosis pathway in renal cell carcinomas. The aim of the present study, therefore, was to investigate the functionality of the mitochondrial apoptosis pathway in renal cell carcinoma cell lines by exposure to TRAIL, etoposide, HA14-1 and betulinic acid activating the mitochondria by different mechanisms. Sensitivity to TRAIL-induced a...
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
Ligands of the mitochondrial 18 kDa translocator protein attenuate apoptosis of human glioblastoma cells exposed to erucylphosphohomocholine.
CONCLUSIONS: This study shows that PK 11195 and Ro5 4864 inhibit the pro-apoptotic function of ErPC3 by blocking its capacity to cause a collapse of the mitochondrial membrane potential. Thus, the TSPO may serve to open the MPTP in response to anti-cancer drugs such as ErPC3.
PMID: 18791274 [PubMed - indexed for MEDLINE] (Source: Analytical Cellular Pathology)
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
Meiotic-like division to a aneuploidy: chromosomal instability (CIN), cell-senescence and cancer.
Authors: Walen KH
PMID: 18791275 [PubMed - indexed for MEDLINE] (Source: Analytical Cellular Pathology)
Source: Analytical Cellular Pathology - November 25, 2014 Category: Cancer & Oncology Tags: Cell Oncol Source Type: research
