Quantification of serine residue stereoinversion in a short peptide by reversed-phase high-performance liquid chromatography: analysis of mechanisms promoting serine stereoinversion
Anal Sci. 2024 Mar 26. doi: 10.1007/s44211-024-00543-5. Online ahead of print.ABSTRACTStereoinversion of Ser residues within proteins, which has been identified in long-lived proteins, influences protein function. To quantify the stereoinversion of Ser residues, we investigated the potential adaptation of our direct peptide analytical method originally established for analyzing the isomerization of asparaginyl/aspartyl residues. Peptide pairs containing L-Ser or D-Ser residues with lengths of four or five residues were synthesized. Separation conditions for these peptide pairs were systematically examined by precisely adju...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 26, 2024 Category: Chemistry Authors: Yutaka Sadakane Mizuki Kobayashi Mitsuteru Sano Shota Morimoto Megumi Hagino Source Type: research

Non-invasive and non-destructive chemical sensing using a wet-interfacing technique
Anal Sci. 2024 Apr;40(4):579-580. doi: 10.1007/s44211-024-00517-7.NO ABSTRACTPMID:38523221 | DOI:10.1007/s44211-024-00517-7 (Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry)
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 25, 2024 Category: Chemistry Authors: Kuniaki Nagamine Source Type: research

Sensing lead ions in water: a comprehensive review on strategies and sensor materials
In conclusion, future directions for chemiresistive sensors in Pb2+ detection are proposed, emphasizing their simplicity, portability, straightforward functionality, and ease of fabrication. Notably, it sheds light on various thiol and ligand compounds and coupling strategies utilized in Pb2+ detection. This comprehensive study is expected to benefit individuals engaged in Pb2+ detection.PMID:38523231 | DOI:10.1007/s44211-024-00547-1 (Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry)
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 25, 2024 Category: Chemistry Authors: Anju Gupta Dinesh Rotake Anand Darji Source Type: research

Detection of the phosphorothioate oligonucleotide fomivirsen using a ligase detection reaction with polymerase chain reaction
This study aimed to develop a simple and sensitive detection method for fomivirsen, a 21-nucleotide phosphorothioate oligonucleotide used as a nucleic acid medicine, using a ligase detection reaction. A ligation probe was designed to hybridize with fomivirsen and polymerase chain reaction (PCR) primers, with a deoxyuridine part between the primer binding sites. The probe was ligated to a circular product by Taq DNA ligase, and the resulting product was converted to a linear form through the removal of the uracil base using uracil DNA glycosylase. The linear product was then quantified using real-time PCR. The developed met...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 25, 2024 Category: Chemistry Authors: Naoki Harikai Haruka Kakuda Takumi Uchiyama Tsubaki Yamamoto Kazumasa Zaima Kazufusa Shinomiya Source Type: research

Non-invasive and non-destructive chemical sensing using a wet-interfacing technique
Anal Sci. 2024 Apr;40(4):579-580. doi: 10.1007/s44211-024-00517-7.NO ABSTRACTPMID:38523221 | DOI:10.1007/s44211-024-00517-7 (Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry)
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 25, 2024 Category: Chemistry Authors: Kuniaki Nagamine Source Type: research

Sensing lead ions in water: a comprehensive review on strategies and sensor materials
In conclusion, future directions for chemiresistive sensors in Pb2+ detection are proposed, emphasizing their simplicity, portability, straightforward functionality, and ease of fabrication. Notably, it sheds light on various thiol and ligand compounds and coupling strategies utilized in Pb2+ detection. This comprehensive study is expected to benefit individuals engaged in Pb2+ detection.PMID:38523231 | DOI:10.1007/s44211-024-00547-1 (Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry)
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 25, 2024 Category: Chemistry Authors: Anju Gupta Dinesh Rotake Anand Darji Source Type: research

Detection of the phosphorothioate oligonucleotide fomivirsen using a ligase detection reaction with polymerase chain reaction
This study aimed to develop a simple and sensitive detection method for fomivirsen, a 21-nucleotide phosphorothioate oligonucleotide used as a nucleic acid medicine, using a ligase detection reaction. A ligation probe was designed to hybridize with fomivirsen and polymerase chain reaction (PCR) primers, with a deoxyuridine part between the primer binding sites. The probe was ligated to a circular product by Taq DNA ligase, and the resulting product was converted to a linear form through the removal of the uracil base using uracil DNA glycosylase. The linear product was then quantified using real-time PCR. The developed met...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 25, 2024 Category: Chemistry Authors: Naoki Harikai Haruka Kakuda Takumi Uchiyama Tsubaki Yamamoto Kazumasa Zaima Kazufusa Shinomiya Source Type: research

A Cu < sup > 2+ < /sup > fluorescent chemosensor suitable for quantitative detection of tyrosinase in potatoes over a wide pH range
In this study, a novel structure fluorescent chemosensor, 1-(6-(((5-(5, 5-difluoro-1, 3, 7, 9-tetramethyl-5H-4λ4, 5λ4-dipyrrolo[1, 2-c:2', 1'-f][1, 3, 2] diazaborinin-10-yl)quinolin-8-yl)oxy)methyl)pyridin-2-yl)-N, N-bis(pyridin-2-ylmethyl)methanamine (1), was synthesized and characterized by 1H and 13C nuclear magnetic resonance spectroscopy, and electrospray ionization mass spectrometry. Sensor 1 showed an obviously "on-off" fluorescence response to Cu2+ with a 1:1 binding stoichiometry by UV-vis and fluorescence spectrophotometry. The detection limit of sensor 1 to Cu2+ was determined to be 1.9 µM, and the stable pH ...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 24, 2024 Category: Chemistry Authors: Xian-Jiao Meng Ya-Ru Zhang Jing-Jing Li Jin-Zhao Wang Jun-Xian Jia Source Type: research

Recent progress in tannin and lignin blended metal oxides and metal sulfides as smart materials for electrochemical sensor applications
Anal Sci. 2024 Mar 22. doi: 10.1007/s44211-024-00544-4. Online ahead of print.ABSTRACTOur technologically advanced civilization has made sensors an essential component. They have potential uses in the pharmaceutical sector, clinical analysis, food quality control, environmental monitoring, and other areas. One of the most active fields of analytical chemistry research is the fabrication of electrochemical sensors. An intriguing area of electroanalytical chemistry is the modification of electrodes using polymeric films. Due to their benefits, which include high adhesion to the electrode surface, chemical stability of the co...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 22, 2024 Category: Chemistry Authors: C Devu S Sreelakshmi R Chandana P Sivanand A Santhy K C Seetha Lakshmi R Rejithamol Source Type: research

Recent progress in tannin and lignin blended metal oxides and metal sulfides as smart materials for electrochemical sensor applications
Anal Sci. 2024 Mar 22. doi: 10.1007/s44211-024-00544-4. Online ahead of print.ABSTRACTOur technologically advanced civilization has made sensors an essential component. They have potential uses in the pharmaceutical sector, clinical analysis, food quality control, environmental monitoring, and other areas. One of the most active fields of analytical chemistry research is the fabrication of electrochemical sensors. An intriguing area of electroanalytical chemistry is the modification of electrodes using polymeric films. Due to their benefits, which include high adhesion to the electrode surface, chemical stability of the co...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 22, 2024 Category: Chemistry Authors: C Devu S Sreelakshmi R Chandana P Sivanand A Santhy K C Seetha Lakshmi R Rejithamol Source Type: research

Recent progress in tannin and lignin blended metal oxides and metal sulfides as smart materials for electrochemical sensor applications
Anal Sci. 2024 Mar 22. doi: 10.1007/s44211-024-00544-4. Online ahead of print.ABSTRACTOur technologically advanced civilization has made sensors an essential component. They have potential uses in the pharmaceutical sector, clinical analysis, food quality control, environmental monitoring, and other areas. One of the most active fields of analytical chemistry research is the fabrication of electrochemical sensors. An intriguing area of electroanalytical chemistry is the modification of electrodes using polymeric films. Due to their benefits, which include high adhesion to the electrode surface, chemical stability of the co...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 22, 2024 Category: Chemistry Authors: C Devu S Sreelakshmi R Chandana P Sivanand A Santhy K C Seetha Lakshmi R Rejithamol Source Type: research

Investigating the influence of solvent type and pH on protein adsorption onto silica surface by evanescent-wave cavity ring-down spectroscopy
This study employs evanescent-wave cavity ring-down spectroscopy (EW-CRDS) to assess the influence of buffer solutions and pH on the adsorption kinetics of three globular proteins: hemoglobin (Hb), myoglobin (Mb), and cytochrome c (Cyt-C) onto fused silica. The EW-CRDS tool, with a ring-down time of 1.4 μ s and a minimum detectable absorbance of 1 × 10 - 6 , enabled precise optical measurements at solid-liquid interfaces. The three heme proteins' adsorption behavior was investigated at pH 7 in three different solvents: deionized (DI) water, tris(hydroxymethyl)-aminomethane hydrochloride (Tris-HCl), and phosphate buff...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 21, 2024 Category: Chemistry Authors: Shadi A Alnaanah Sergio B Mendes Source Type: research

Tag-free protein modification by lipoate ligase A: exploring substrate tolerance
This study delves into the functional intricacies of lipoate ligase A (LplA), an enzyme showing great promise in bioconjugation due to its unique capacity for introducing azido groups into proteins without requiring a genetic tag. We aimed to enhance the understanding of LplA's functionality, particularly its substrate tolerance and the reliability of various analytical techniques. A pivotal aspect of our approach was incorporating azido groups into a range of proteins, followed by the addition of the fluorescent molecule Cy3 via click chemistry. Analysis of fluorescent intensity in the altered proteins indicated varying d...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 20, 2024 Category: Chemistry Authors: Shunsuke Yamazaki Kazutoshi Takahashi Yutaka Matsuda Source Type: research

LC-MS/MS analysis of components in smoke from e-cigarettes that use guarana extract as the caffeine source
In this study, an LC-MS/MS analysis of components in the smoke from e-cigarettes with GE was performed. The concentration ranges of Caf and the minor components theophylline (TP), theobromine (TB), and paraxanthine (PX) in e-liquid and cigarette smoke extract (CSE) of five e-cigarette products were determined. The concentration ranges of e-liquid and CSE were 2.17-8.62 mg/mL and 0.17-1.17 µg/puff for Caf, 0.09-37.58 µg/mL and 0.03-11.88 ng/puff for TB, 50.28-185.26 ng/mL and 0.00-0.05 ng/puff for TP, and 0.44-4.09 µg/mL and 0.03-0.20 ng/puff for PX, respectively. By comparing the peak area ratios of e-liquid and CSE, we...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 20, 2024 Category: Chemistry Authors: Suzuna Saruwatari Makoto Takada Junpei Mutoh Naoya Kishikawa Naotaka Kuroda Mitsuhiro Wada Source Type: research

Tag-free protein modification by lipoate ligase A: exploring substrate tolerance
This study delves into the functional intricacies of lipoate ligase A (LplA), an enzyme showing great promise in bioconjugation due to its unique capacity for introducing azido groups into proteins without requiring a genetic tag. We aimed to enhance the understanding of LplA's functionality, particularly its substrate tolerance and the reliability of various analytical techniques. A pivotal aspect of our approach was incorporating azido groups into a range of proteins, followed by the addition of the fluorescent molecule Cy3 via click chemistry. Analysis of fluorescent intensity in the altered proteins indicated varying d...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - March 20, 2024 Category: Chemistry Authors: Shunsuke Yamazaki Kazutoshi Takahashi Yutaka Matsuda Source Type: research