Analytical and Bioanalytical Chemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Development and performance of NLISA for C-reactive protein detection based on Prussian blue nanoparticle conjugates
Anal Bioanal Chem. 2024 Apr 18. doi: 10.1007/s00216-024-05268-y. Online ahead of print.ABSTRACTPrussian blue nanoparticles (PBNPs), also called nanozymes, are very attractive as an alternative to horseradish peroxidase in immunoassay development due to their simple and low-cost synthesis, stability and high catalytic activity. Today, there is a method for highly effective PBNP synthesis based on the reduction of an FeCl3/K3[Fe(CN)6] mixture by hydrogen peroxide. However, there is a lack of research showcasing the use of these highly effective PBNPs for specific target detection in clinical settings, as well as a lack of co...
Source: Analytical and Bioanalytical Chemistry - April 18, 2024 Category: Chemistry Authors: Maria Nikitina Pavel Khramtsov Maria Bochkova Mikhail Rayev Source Type: research

A lateral flow assay for miRNA-21 based on CRISPR/Cas13a and MnO < sub > 2 < /sub > nanosheets-mediated recognition and signal amplification
In this study, a lateral flow assay based on the CRISPR/Cas13a system and MnO2 nanozyme was developed for highly sensitive detection of microRNA-21 (miR-21). The CRISPR/Cas13a cleavage system exhibits the ability to recognize the specific oligonucleotide sequence, where two-base mismatches significantly impact the cleavage activity of the Cas13a. Upon binding of the target to crRNA, the cleavage activity of Cas13a is activated, resulting in the unlocking of the sequence and initiating strand displacement, thereby enabling signal amplification to produce a new sequence P1. When applying the reaction solution to the lateral ...
Source: Analytical and Bioanalytical Chemistry - April 17, 2024 Category: Chemistry Authors: Mingyuan Wang Shixin Cai Yunqing Wu Qi Li Xiaoli Wang Yuting Zhang Nandi Zhou Source Type: research

MALDI-mass spectrometry imaging as a new technique for detecting non-heme iron in peripheral tissues via caudal vein injection of deferoxamine
Anal Bioanal Chem. 2024 Apr 17. doi: 10.1007/s00216-024-05289-7. Online ahead of print.ABSTRACTAs one of the most common iron-chelating agents, deferoxamine (DFO) rapidly chelates iron in the body. Moreover, it does not compete for the iron characteristic of hemoglobin in the blood cells, which is common in the clinical treatment of iron poisoning. Iron is a trace element necessary to maintain organism normal life activities. Iron deficiency can lead to anemia, whereas iron overload can cause elevated levels of cellular oxidative stress and cell damage. As a consequence, detection of the iron content in tissues and blood i...
Source: Analytical and Bioanalytical Chemistry - April 17, 2024 Category: Chemistry Authors: Xiaofang Jin Xintong Shi Tong Zhang Xingyao Li Yajing Xie Siyu Tian Kang Han Source Type: research

Paper-based fluorescence sensor array with functionalized carbon quantum dots for bacterial discrimination using a machine learning algorithm
Anal Bioanal Chem. 2024 Apr 17. doi: 10.1007/s00216-024-05262-4. Online ahead of print.ABSTRACTThe rapid discrimination of bacteria is currently an emerging trend in the fields of food safety, medical detection, and environmental observation. Traditional methods often require lengthy culturing processes, specialized analytical equipment, and bacterial recognition receptors. In response to this need, we have developed a paper-based fluorescence sensor array platform for identifying different bacteria. The sensor array is based on three unique carbon quantum dots (CQDs) as sensing units, each modified with a different antibi...
Source: Analytical and Bioanalytical Chemistry - April 17, 2024 Category: Chemistry Authors: Fangbin Wang Minghui Xiao Jing Qi Liang Zhu Source Type: research

Mapping elemental solutes at sub-picogram levels during aqueous corrosion of Al alloys using diffusive gradients in thin films (DGT) with LA-ICP-MS
Anal Bioanal Chem. 2024 Apr 16. doi: 10.1007/s00216-024-05288-8. Online ahead of print.ABSTRACTA novel approach using diffusive gradients in thin films (DGT) with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for two-dimensional mapping of elemental solute release at sub-picogram levels during aqueous corrosion of Al alloys is presented. Evaluation of different DGT gels with mixed micro-sized binding phases (polyacrylamide-Chelex-Metsorb, polyurethane (PU)-Chelex-Metsorb, PU-Chelex-Zr(OH)4) demonstrated the superior performance of PU gels due to their tear-proof handling, low shrinkage, and compli...
Source: Analytical and Bioanalytical Chemistry - April 16, 2024 Category: Chemistry Authors: Gulnaz Mukhametzianova Stefan Wagner Magdalena Eskinja Masoud Moshtaghi Gregor Mori Thomas Prohaska Source Type: research

A sensitive fluorescence biosensor based on ligation-transcription and CRISPR/Cas13a-assisted cascade amplification strategies to detect the H1N1 virus
Anal Bioanal Chem. 2024 Apr 13. doi: 10.1007/s00216-024-05269-x. Online ahead of print.ABSTRACTWe propose a sensitive H1N1 virus fluorescence biosensor based on ligation-transcription and CRISPR/Cas13a-assisted cascade amplification strategies. Products are generated via the hybridization of single-stranded DNA (ssDNA) probes containing T7 promoter and crRNA templates to a target RNA sequence using SplintR ligase. This generates large crRNA quantities in the presence of T7 RNA polymerase. At such crRNA quantities, ternary Cas13a, crRNA, and activator complexes are successfully constructed and activate Cas13a to enhance flu...
Source: Analytical and Bioanalytical Chemistry - April 13, 2024 Category: Chemistry Authors: Lulu Xue Shengjun Bu Mengyao Xu Jiaqi Wei Hongyu Zhou Yao Xu Zhuo Hao Zehong Li Jiayu Wan Source Type: research

Tailoring the d-band center on Ru < sub > 1 < /sub > Cu single-atom alloy nanotubes for boosting electrochemical non-enzymatic glucose sensing
Anal Bioanal Chem. 2024 Apr 13. doi: 10.1007/s00216-024-05284-y. Online ahead of print.ABSTRACTThe development of cost-effective and highly efficient electrocatalysts is critical to help electrochemical non-enzymatic sensors achieve high performance. Here, a new class of catalyst, Ru single atoms confined on Cu nanotubes as a single-atom alloy (Ru1Cu NTs), with a unique electronic structure and property, was developed to construct a novel electrochemical non-enzymatic glucose sensor for the first time. The Ru1Cu NTs with a diameter of about 24.0 nm showed a much lower oxidation potential (0.38 V) and 9.0-fold higher respon...
Source: Analytical and Bioanalytical Chemistry - April 13, 2024 Category: Chemistry Authors: Shuang Zhang Yunhao Jiang Wenli Lei Yueming Zhai Juejing Liu Xingyi Lyu Tao Li Xiaofeng Guo Yuanmeng Zhao Changsheng Shan Li Niu Source Type: research

Recent progress on charge transfer engineering in reticular framework for efficient electrochemiluminescence
Anal Bioanal Chem. 2024 Apr 13. doi: 10.1007/s00216-024-05279-9. Online ahead of print.ABSTRACTElectrochemiluminescence (ECL) is a luminescence production technique triggered by electrochemistry, which has emerged as a powerful analytical technique in bioanalysis and clinical diagnosis. During ECL, charge transfer (CT) is an important process between electrochemical excitation and luminescent emission, and dramatically affects the efficiency of exciton generation, playing a pivotal role in the light-emitting properties of nanomaterials. Reticular framework materials with intramolecular/intermolecular interactions offer a p...
Source: Analytical and Bioanalytical Chemistry - April 13, 2024 Category: Chemistry Authors: Xinzhou Huang Qian Sun Jinjin Zhao Guoqiu Wu Yuanjian Zhang Yanfei Shen Source Type: research

An amino-rich polymer-coated magnetic nanomaterial for ultra-rapid separation of phosphorylated peptides in the serum of Parkinson's disease patients
Anal Bioanal Chem. 2024 Apr 12. doi: 10.1007/s00216-024-05287-9. Online ahead of print.ABSTRACTThe elucidation of disease pathogenesis can be achieved by analyzing the low-abundance phosphopeptides in organisms. Herein, we developed a novel and easy-to-prepare polymer-coated nanomaterial. By improving the hydrophilicity and spatial conformation of the material, we effectively enhanced the adsorption of phosphopeptides and demonstrated excellent enrichment properties. The material was able to successfully enrich the phosphopeptides in only 1 min. Meanwhile, the material has high selectivity (1:2000), good loading capacity (...
Source: Analytical and Bioanalytical Chemistry - April 12, 2024 Category: Chemistry Authors: Xiaoya Zhang Bing Wang Yiting Luo Chuan-Fan Ding Yinghua Yan Source Type: research

Python workflow for the selection and identification of marker peptides-proof-of-principle study with heated milk
Anal Bioanal Chem. 2024 Apr 12. doi: 10.1007/s00216-024-05286-w. Online ahead of print.ABSTRACTThe analysis of almost holistic food profiles has developed considerably over the last years. This has also led to larger amounts of data and the ability to obtain more information about health-beneficial and adverse constituents in food than ever before. Especially in the field of proteomics, software is used for evaluation, and these do not provide specific approaches for unique monitoring questions. An additional and more comprehensive way of evaluation can be done with the programming language Python. It offers broad possibil...
Source: Analytical and Bioanalytical Chemistry - April 12, 2024 Category: Chemistry Authors: Gesine Kuhnen Lisa-Carina Class Svenja Badekow Kim Lara Hanisch Sascha Rohn J ürgen Kuballa Source Type: research