Analytical and Bioanalytical ChemistryThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

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The importance of asking, mentoring and building networks for academic career success - a personal and social science perspective.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19921155 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - November 18, 2009 Category: Chemistry Authors: Stenken JA, Zajicek AM Tags: Anal Bioanal Chem Source Type: journals

Determination of vanadium(V) in the particulate matter of emissions and working areas by sequential dissolution and solid-phase extraction.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A method based on selective sequential dissolutions is proposed to determine total vanadium(V) in particulate matter of emissions and working areas at concentrations 1,000 times lower than the threshold limit of 0.05 mg m(-3). Separation and preconcentration of vanadium(V) has been achieved by solid-phase extraction on Chelex 100 resin. Possible influence of the matrix has been investigated for two standard reference materials (SRMs), NIST SRM 1648 and BCR-038, before and after spiking, with vanadium(V) recovery in the range 97-103%. PMID: 19921508 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - November 18, 2009 Category: Chemistry Authors: Sturini M, Maraschi F, Cucca L, Spini G, Talamini G, Profumo A Tags: Anal Bioanal Chem Source Type: journals

Magnetic-based purification system with simultaneous sample washing and concentration.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Simultaneous washing and concentration of magnetic microparticles was demonstrated using a rotational magnetic system under a continuous-flow condition. The rotation of periodically arranged permanent magnets close to a fluidic channel carrying a suspension of magnetic particles allows the trapping and releasing of particles along the fluidic channel in a periodic manner. Each trapping and releasing event resembles one washing cycle in conventional biological assays. Concentration efficiencies of 99.75 +/- 0.083% at a flow rate of 200 microl/min and 88.10 +/- 3.17% at a flow rate of 1,000 microl/min and a purification ...
Source: Analytical and Bioanalytical Chemistry - November 18, 2009 Category: Chemistry Authors: Ramadan Q, Lau TT, Ho SB Tags: Anal Bioanal Chem Source Type: journals

Determination of 19 drugs of abuse and metabolites in whole blood by high-performance liquid chromatography-tandem mass spectrometry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A high-performance liquid chromatography (LC)-tandem mass spectrometry (MS/MS) method has been developed and validated for the determination of 19 drugs of abuse and metabolites and used in whole blood. The following compounds were included: amphetamine, methylenedioxyamphetamine, methylenedioxyethylamphetamine, methylenedioxymethamphetamine, methamphetamine, cocaine, benzoylecgonine, morphine, 6-acetylmorphine, codeine, methadone, buprenorphine, norbuprenorphine, ketobemidone, tramadol, O-desmethyltramadol, zaleplone, zolpidem, and zopiclone. The sample pretreatment consisted of solid-phase extraction using mixed-mode...
Source: Analytical and Bioanalytical Chemistry - November 18, 2009 Category: Chemistry Authors: Bjørk MK, Nielsen MK, Markussen LO, Klinke HB, Linnet K Tags: Anal Bioanal Chem Source Type: journals

Development of analytical procedures for trace-level determination of polybrominated diphenyl ethers and tetrabromobisphenol A in river water and sediment.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this work was to develop procedures for the simultaneous determination of selected brominated flame retardants (BFRs) in river water and in river bed sediment. The target analytes were polybrominated diphenyl ethers (PBDEs) and tetrabromobisphenol A (TBBPA). To determine dissolved BFRs, a novel mixed-mode solid-phase extraction procedure was developed by combining a hydrophobic sorbent (C(18)) with a silica-based anion exchange sorbent, so as to overcome the negative artefact induced by dissolved organic carbon. Extraction recoveries exceeded 73% for most analytes, except for BDE-183 and BDE-209 (57%). As re...
Source: Analytical and Bioanalytical Chemistry - November 18, 2009 Category: Chemistry Authors: Labadie P, Tlili K, Alliot F, Bourges C, Desportes A, Chevreuil M Tags: Anal Bioanal Chem Source Type: journals

Development of a chemiluminescent ELISA and a colloidal gold-based LFIA for TNT detection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To identify the explosive used in a terrorist attack, or to obtain an early sign of environmental pollution it is important to use simple and rapid assays able to detect analytes at low levels, possibly on-site. This is particularly true for TNT (2,4,6-trinitrotoluene), one of the most employed explosives in the 20th century and at the same time, because of its toxicity, a well known pollutant. In this work we describe the development of an indirect competitive ELISA with chemiluminescent detection (CL-ELISA) and of a lateral-flow immunoassay (LFIA) based on colloidal gold nanoparticle labels. A commercially available ...
Source: Analytical and Bioanalytical Chemistry - November 18, 2009 Category: Chemistry Authors: Girotti S, Eremin S, Montoya A, Moreno MJ, Caputo P, D'Elia M, Ripani L, Romolo FS, Maiolini E Tags: Anal Bioanal Chem Source Type: journals

Standardization of ceruloplasmin measurements is still an issue despite the availability of a common reference material.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The purpose of measurement standardization is to achieve closer comparability of results obtained using different commercial systems. Regarding serum protein immunoassays, a reference preparation (BCR-470) was released in 1993 and adopted by manufacturers across the world to value-assign their assay calibrators for routine methods to reduce method-dependent variation. Moving from nephelometric (Beckman Immage 800) to turbidimetric determination (Roche Cobas c 501) of seven serum proteins, we preliminarily checked the comparability of results between the two systems. The study was performed according to the CLSI EP9-A p...
Source: Analytical and Bioanalytical Chemistry - November 17, 2009 Category: Chemistry Authors: Infusino I, Valente C, Dolci A, Panteghini M Tags: Anal Bioanal Chem Source Type: journals

On optical depth profiling using confocal Raman spectroscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Until 2006 the performance of confocal Raman spectroscopy depth profiling was typically described and modeled through the application of geometrical optics, including refraction at the surface, to explain the degree of resolution and the precise form of the depth profile obtained from transparent and semicrystalline materials. Consequently a range of techniques, physical and analytical, was suggested to avoid the errors thus encountered in order to improve the practice of Raman spectroscopy, if not the understanding of the underlying mechanisms. These approaches were completely unsuccessful in accounting for the precis...
Source: Analytical and Bioanalytical Chemistry - November 15, 2009 Category: Chemistry Authors: Freebody NA, Vaughan AS, Macdonald AM Tags: Anal Bioanal Chem Source Type: journals

Nanostructured optical fibre arrays for high-density biochemical sensing and remote imaging.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Optical fibre bundles usually comprise a few thousand to tens of thousands of individually clad glass optical fibres. The ordered arrangement of the fibres enables coherent transmission of an image through the bundle and therefore enables analysis and viewing in remote locations. In fused bundles, this architecture has also been used to fabricate arrays of various micro to nano-scale surface structures (micro/nanowells, nanotips, triangles, etc.) over relatively large areas. These surface structures have been used to obtain new optical and analytical capabilities. Indeed, the imaging bundle can be thought of as a "star...
Source: Analytical and Bioanalytical Chemistry - November 15, 2009 Category: Chemistry Authors: Deiss F, Sojic N, White DJ, Stoddart PR Tags: Anal Bioanal Chem Source Type: journals

Investigation of urinary steroid metabolites in calf urine after oral and intramuscular administration of DHEA.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
DHEA (3beta-hydroxy-androst-5-en-17-one) is a natural steroid prohormone. Despite a lack of information on the effect, DHEA and other prohormones are frequently used as a food supplement by body-builders. DHEA is suspected for growth promoting abuse in cattle as well. Considering the latter, urine samples from a previous exposure study in which calves were exposed to 1 g DHEA per day for 7 days, were used. The calves were divided in three groups: one orally treated, one intramuscularly injected, and a control group. The effect of this treatment on the urinary profile of several precursors and metabolites of DHEA was in...
Source: Analytical and Bioanalytical Chemistry - November 14, 2009 Category: Chemistry Authors: Becue I, Van Poucke C, Rijk JC, Bovee TF, Nielen M, Van Peteghem C Tags: Anal Bioanal Chem Source Type: journals

Genotoxic potential of TiO(2) on bottlenose dolphin leukocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Titanium dioxide is extensively used in a variety of products, including industrial materials and cosmetics. Studies mainly performed on human cell lines and in vivo exposure on experimental animals have raised concern about the toxic effects of ultrafine titanium dioxide; however, scarce information is available about its impact on aquatic life. The aim of this article was to assess the genotoxic potential of TiO(2) (anatase and rutile) on bottlenose dolphin leukocytes. Blood samples were obtained from four male and one female specimens reared at the Adriatic SeaWorld "Oltremare" (Riccione, Italy). Leukocytes were iso...
Source: Analytical and Bioanalytical Chemistry - November 14, 2009 Category: Chemistry Authors: Bernardeschi M, Guidi P, Scarcelli V, Frenzilli G, Nigro M Tags: Anal Bioanal Chem Source Type: journals

James S. Fritz, Douglas T. Gjerde: Ion Chromatography, 4(th) ed.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19915827 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - November 14, 2009 Category: Chemistry Authors: Haddad P Tags: Anal Bioanal Chem Source Type: journals

Thoughts on managing the analytical laboratory.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19911171 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - November 13, 2009 Category: Chemistry Authors: Turle R Tags: Anal Bioanal Chem Source Type: journals

Analytical tools for the nanoworld.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19911172 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - November 13, 2009 Category: Chemistry Authors: Zenobi R Tags: Anal Bioanal Chem Source Type: journals

Immunoassay detection in a perfluorocarbon emulsion oxygen therapeutic.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The effect of a perfluorocarbon emulsion oxygen therapeutic (PEOT) on detecting theophylline was explored using a commercial EMIT(R) (enzyme multiplied immunoassay technique) immunoassay. The EMIT technique is based on a colorimetric reaction, the product of which can be measured spectrophotometrically. The intent was to determine whether the presence of PEOT interferes with this detection. We found that the immunoassay yields excellent quantitative data in the therapeutic range (10-20 ppm theophylline) in 2-10% PEOT, but as the amount of PEOT in the sample increases, the accuracy of the detection method decreases. ...
Source: Analytical and Bioanalytical Chemistry - November 13, 2009 Category: Chemistry Authors: Barlag RE, Halsall HB, Heineman WR Tags: Anal Bioanal Chem Source Type: journals

Laser desorption/ionization mass spectrometry analysis of monolayer-protected gold nanoparticles.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monolayer-protected gold nanoparticles (AuNPs) feature unique surface properties that enable numerous applications. Thus, there is a need for simple, rapid, and accurate methods to confirm the surface structures of these materials. Here, we describe how laser desorption/ionization mass spectrometry (LDI-MS) can be used to characterize AuNPs with neutral, positively, and negatively charged surface functional groups. LDI readily desorbs and ionizes the gold-bound ligands to produce both free thiols and disulfide ions in pure and complex samples. We also find that LDI-MS can provide a semi-quantitative measure of the liga...
Source: Analytical and Bioanalytical Chemistry - November 13, 2009 Category: Chemistry Authors: Yan B, Zhu ZJ, Miranda OR, Chompoosor A, Rotello VM, Vachet RW Tags: Anal Bioanal Chem Source Type: journals

Immuno-interferometric sensor for the detection of influenza A nucleoprotein.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Influenza A virus, both seasonal and pandemic, has the potential to cause rampant devastating disease around the world. The most relied upon methods of viral detection require days, skilled workers, and laboratory settings to complete properly. Here, we report two methods for the detection of the nucleoprotein from inactivated influenza A (IFA-NP), a patented polymer-protein antibody orientation immuno-method, termed ALYNGSA, and a newly fabricated optical label-free Fabry-Perot interferometric immunosensor. The ALYGNSA assay for IFA-NP had a level of detection below 5 mug/mL of inactivated virus sample. The label-free...
Source: Analytical and Bioanalytical Chemistry - November 13, 2009 Category: Chemistry Authors: Farris LR, Wu N, Wang W, Clarizia LJ, Wang X, McDonald MJ Tags: Anal Bioanal Chem Source Type: journals

Knowledge-technology-based discovery of unauthorized genetically modified organisms.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19911176 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - November 13, 2009 Category: Chemistry Authors: Ruttink T, Morisset D, Van Droogenbroeck B, Lavrač N, Van Den Eede GL, Zel J, De Loose M Tags: Anal Bioanal Chem Source Type: journals

Performance evaluation of mapping and linear imaging FTIR microspectroscopy for the characterisation of paint cross sections.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Different Fourier transform infrared microspectroscopic techniques, using attenuated total reflection (ATR) mode and single-element mercury-cadmium-telluride (MCT) detector (mapping) or multielement MCT detector (raster scanning), are compared with each other for the characterisation of inorganic compounds and organic substances in paint cross sections. All measurements have been performed on paint cross sections embedded in potassium bromide, a transparent salt in the mid-infrared region, in order to better identify the organic materials without the interference of the usual embedding resin. The limitations and advant...
Source: Analytical and Bioanalytical Chemistry - November 12, 2009 Category: Chemistry Authors: Joseph E, Prati S, Sciutto G, Ioele M, Santopadre P, Mazzeo R Tags: Anal Bioanal Chem Source Type: journals

Microcavities: tailoring the optical properties of single quantum emitters.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present a general review of different microresonator structures and how they can be used in future device applications in modern analytical methods by tailoring the optical properties of single quantum emitters. The main emphasis is on the tunable lambda/2-Fabry-Perot-type microresonator which we used to obtain the results presented in this article. By varying the mirror distance the local mode structure of the electromagnetic field is altered and thus the radiative coupling of fluorescent single quantum emitters embedded inside the resonator to that field is changed, too. As a result a modification of the optical prope...
Source: Analytical and Bioanalytical Chemistry - November 12, 2009 Category: Chemistry Authors: Bär S, Chizhik A, Gutbrod R, Schleifenbaum F, Chizhik A, Meixner AJ Tags: Anal Bioanal Chem Source Type: journals

DGMS 2009: focus on bioanalytical mass spectrometry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19908032 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - November 12, 2009 Category: Chemistry Authors: Przybylski M Tags: Anal Bioanal Chem Source Type: journals

Two-dimensional liquid chromatography of synthetic polymers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Two-dimensional liquid chromatography, 2D-LC of synthetic polymers is critically assessed. Similarities and differences of 2D-LC of low-molecular-mass and polymeric substances are reviewed. The rationale of application of 2D-LC to macromolecular substances is discussed. Basic information on retention mechanisms in liquid chromatography of synthetic polymers is furnished. The principles, reasons, and significance of coupling of retention mechanisms are explained. The resulting separation processes are elucidated, and the technical concepts of the corresponding experimental arrangements are described. The benefits of 2D-...
Source: Analytical and Bioanalytical Chemistry - November 12, 2009 Category: Chemistry Authors: Berek D Tags: Anal Bioanal Chem Source Type: journals

Effects of 17beta-trenbolone in male eelpout Zoarces viviparus exposed to ethinylestradiol.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study shows that lower concentrations of 17beta-trenbolone are unable to counteract the EE2 estrogenic effects when the exposure is simultaneous. PMID: 19902185 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - November 10, 2009 Category: Chemistry Authors: Velasco-Santamaría YM, Madsen SS, Bjerregaard P, Korsgaard B Tags: Anal Bioanal Chem Source Type: journals

Erratum to: Immuno-PCR-based quantification of multiple phosphorylated tau-epitopes linked to Alzheimer's disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19902186 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - November 10, 2009 Category: Chemistry Authors: Singer D, Soininen H, Alafuzoff I, Hoffmann R Tags: Anal Bioanal Chem Source Type: journals

Toxicities of nano zinc oxide to five marine organisms: influences of aggregate size and ion solubility.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study primarily aimed to characterize the aggregate size and solubility of nZnO and bulk ZnO, and to assess their toxicities towards five selected marine organisms. Chemical characterization showed that nZnO formed larger aggregates in seawater than ZnO, while nZnO had a higher solubility in seawater (3.7 mg L(-1)) than that of ZnO (1.6 mg L(-1)). Acute tests were conducted using the marine diatoms Skeletonema costatum and Thalassiosia pseudonana, the crustaceans Tigriopus japonicus and Elasmopus rapax, and the medaka fish Oryzias melastigma. In general, nZnO was more toxic towards algae than ZnO, but relatively less ...
Source: Analytical and Bioanalytical Chemistry - November 10, 2009 Category: Chemistry Authors: Wong SW, Leung PT, Djurišić AB, Leung KM Tags: Anal Bioanal Chem Source Type: journals

Small-volume fiber-optic evanescent-wave absorption sensor for nitrite determination.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A novel small-volume fiber-optic evanescent-wave absorption sensor based on the Griess-Ilosvay reaction has been developed and evaluated for nitrite determination. The sensor was constructed by inserting a decladded optical fiber into a transparent capillary to form an annular column microchannel. The Evanescent wave (EW) field produced on the optical fiber core surface penetrated into the surrounding medium and interacted with the azo dye, which was generated by the reaction of nitrite and nitrite-sensitive reagents. The detector was designed to be parallel to the axis of the optical fiber. The defined absorbance was ...
Source: Analytical and Bioanalytical Chemistry - November 10, 2009 Category: Chemistry Authors: Xiong Y, Zhu DQ, Duan CF, Wang JW, Guan YF Tags: Anal Bioanal Chem Source Type: journals

A new ozone denuder for aerosol sampling based on an ionic liquid coating.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A new ionic liquid 1-octyl-3,5-dimethylpyridinium iodide ([O35LUT](+)[I](-)) was synthesized and utilized as coating for an ozone denuder device based on a high-volume aerosol sampler (30 m(3) h(-1)). Particle transmission of the denuder was studied, and over 99% of particles ranging from 10 to 2,500 nm were transmitted. The device, containing 4.66 g of ionic liquid, was used outdoors, under dry and damp atmospheric conditions. In order to expose the device to an average concentration of 120 ppbv (240 microg m(-3)) of ozone in air, an additional production of ozone was directly injected into the denuder. Under these co...
Source: Analytical and Bioanalytical Chemistry - November 10, 2009 Category: Chemistry Authors: Albinet A, Papaiconomou N, Estager J, Suptil J, Besombes JL Tags: Anal Bioanal Chem Source Type: journals

Use of liquid chromatography coupled to low- and high-resolution linear ion trap mass spectrometry for studying the metabolism of paynantheine, an alkaloid of the herbal drug Kratom in rat and human urine.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, PAY and its metabolites may be further markers for a Kratom abuse in addition of MG and its metabolites. PMID: 19902190 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - November 10, 2009 Category: Chemistry Authors: Philipp AA, Wissenbach DK, Weber AA, Zapp J, Zoerntlein SW, Kanogsunthornrat J, Maurer HH Tags: Anal Bioanal Chem Source Type: journals

Phenolic characterization of Northeast Portuguese propolis: usual and unusual compounds.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, an ethanolic extract from Portuguese propolis was prepared, fractionated by high-performance liquid chromatography, and the identification of the phenolic compounds was done by electrospray mass spectrometry in the negative mode. This technical approach allowed the identification of 37 phenolic compounds, which included not only the typical phenolic acids and flavonoids found in propolis from temperate zones but also several compounds in which its occurrence have never been referred to in the literature. Four of the novel phenolic compounds were methylated and/or esterified or hydroxylated derivatives of com...
Source: Analytical and Bioanalytical Chemistry - November 10, 2009 Category: Chemistry Authors: Falcão SI, Vilas-Boas M, Estevinho LM, Barros C, Domingues MR, Cardoso SM Tags: Anal Bioanal Chem Source Type: journals

Improved liquid chromatography-tandem mass spectrometry method in clinical utility for the diagnosis of Cushing's syndrome.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Determination of urinary free cortisol is one of the first lines in screening for the diagnosis of Cushing's syndrome where its measurement is mostly done by immunoassay. Although easy to perform, immunoassays suffer from the problem of assay interferences and are unable to measure cortisone levels. To enhance such techniques for clinical diagnosis, an improved liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed for the simultaneous determination of urinary free cortisol and cortisone. The leftover urine samples from immunoassay were collected and subjected to facile solid-phase extraction c...
Source: Analytical and Bioanalytical Chemistry - November 10, 2009 Category: Chemistry Authors: Fong BM, Tam S, Leung KS Tags: Anal Bioanal Chem Source Type: journals

Development and validation of a simultaneous extraction procedure for HPLC-MS quantification of daptomycin, amikacin, gentamicin, and rifampicin in human plasma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A simultaneous extraction method to measure daptomycin, amikacin, gentamicin, and rifampicin in human plasma, by high-performance liquid chromatography, was developed and validated. The method involved a rapid sample preparation by protein precipitation with acetonitrile followed by direct injection into a high-performance liquid chromatography system coupled with mass detection. Drug retention times were 10.00 +/- 0.25, 2.00 +/- 0.25, 3.50 +/- 0.25, 11.50 +/- 0.25, and 12.50 +/- 0.25 min for daptomycin, amikacin, gentamicin, rifampicin, and quinoxaline, respectively. Good linearity (mean r (2) = 0.998) was obtained fo...
Source: Analytical and Bioanalytical Chemistry - November 9, 2009 Category: Chemistry Authors: Baietto L, D'Avolio A, De Rosa FG, Garazzino S, Michelazzo M, Ventimiglia G, Siccardi M, Simiele M, Sciandra M, Di Perri G Tags: Anal Bioanal Chem Source Type: journals

A microfluidic device with integrated fluorimetric detection for flow injection analysis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This work describes the development of flow analysis microsystems with integrated fluorimetric detection cells. Channels (width of 300-540 microm and depth of 200-590 microm) were manufactured by deep-UV lithography in urethane-acrylate (UA) resin. Plastic optical fibers (diameter of 250 microm) were coupled to a 2.0-mm-long detection channel in order to guide the excitation radiation from an LED (470 nm) and collect the emitted radiation at a right angle towards a photomultiplier. A single-line miniaturized system, with a total internal volume of 10.4 muL, was evaluated by means of standard fluorescein solutions (0.53...
Source: Analytical and Bioanalytical Chemistry - November 8, 2009 Category: Chemistry Authors: Fonseca A, Raimundo IM, Rohwedder JJ, Lima RS, Araújo MC Tags: Anal Bioanal Chem Source Type: journals

Identification of a CYP3A form (CYP3A126) in fathead minnow (Pimephales promelas) and characterisation of putative CYP3A enzyme activity.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we report on the cloning of a CYP3A family gene from fathead minnows (Pimephales promelas), which has been designated as CYP3A126 by the P450 nomenclature committee (GenBank no. EU332792). The cDNA was isolated, identified and characterised by extended inverse polymerase chain reaction (PCR), an alternative to the commonly used method of rapid amplification of cDNA ends. In a fathead minnow cell line we identified a full-length cDNA sequence (1,863 base pairs (bp)) consisting of a 1,536 bp open reading frame encoding a 512 amino acid protein. Genomic analysis of the identified CYP3A isoenzyme revealed a DNA s...
Source: Analytical and Bioanalytical Chemistry - November 8, 2009 Category: Chemistry Authors: Christen V, Caminada D, Arand M, Fent K Tags: Anal Bioanal Chem Source Type: journals

Polymer-protein-enhanced fluoroimmunoassay for prostate-specific antigen.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Prostate-specific antigen (PSA) is a serum glycoprotein overproduced in prostate cancer, the total of which is comprised of two major forms, free and complexed. The common method for measuring of PSA is an Enzyme-linked immunosorbent assay (ELISA). Limits of detection using commercial PSA ELISA kits for free and total PSA were determined in our laboratory to be 1 and 10 ng/mL, respectively. A value of 0.10 was obtained for the free to total PSA ratio, a ratio of 0.25 being indicative of prostate cancer. A possible improvement in the sensitivity and detection limits of free and total PSA may be achieved by the ALYGNSA s...
Source: Analytical and Bioanalytical Chemistry - November 8, 2009 Category: Chemistry Authors: Mackness BC, Chourb S, Farris LR, McDonald MJ Tags: Anal Bioanal Chem Source Type: journals

Fumonisins determination in urine by LC-MS-MS.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present work shows the optimization and validation results of an analytical methodology based on imunoaffinity clean-up (IAC) followed by liquid chromatography with tandem mass detection (LC-MS-MS) for the analysis of the mycotoxins fumonisin B(1) (FB(1)) and B(2) (FB(2)) in human urine samples, in order to evaluate fumonisins exposure in two Portuguese populations. As far as we know, imunoaffinity clean-up procedure was used, for the first time, in the analysis of fumonisins (FBs) in urine. Using this analytical methodology, the limit of quantification achieved was 10 ng mL(-1) for FB(1) and for FB(2). Recoveries ...
Source: Analytical and Bioanalytical Chemistry - November 8, 2009 Category: Chemistry Authors: Silva LJ, Pena A, Lino CM, Fernández MF, Mañes J Tags: Anal Bioanal Chem Source Type: journals

Single-walled carbon nanotube as an effective quencher.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Over the past few years, single-walled carbon nanotubes (SWNTs) have been the focus of intense research motivated by their unique physical and chemical properties. This review specifically summarizes recent progress in the development of fluorescence biosensors that integrate the quenching property of SWNTs and the recognition property of functional nucleic acids. SWNTs are substantially different from organic quenchers, showing superior quenching efficiency for a variety of fluorophores, with low background and high signal-to-noise ratio, as well as other advantages derived from the nanomaterial itself. As the second ...
Source: Analytical and Bioanalytical Chemistry - November 8, 2009 Category: Chemistry Authors: Zhu Z, Yang R, You M, Zhang X, Wu Y, Tan W Tags: Anal Bioanal Chem Source Type: journals

Damià Barceló, Peter-Diedrich Hansen (Eds.): Biosensors for the environmental monitoring of aquatic systems. Bioanalytical and chemical methods for endocrine disruptors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Damià Barceló, Peter-Diedrich Hansen (Eds.): Biosensors for the environmental monitoring of aquatic systems. Bioanalytical and chemical methods for endocrine disruptors. Anal Bioanal Chem. 2009 Nov 7; Authors: Buszewski B PMID: 19898837 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - November 7, 2009 Category: Chemistry Authors: Buszewski B Tags: Anal Bioanal Chem Source Type: journals

Improved procedure for protein binder analysis in mural painting by LC-ESI/Q-q-TOF mass spectrometry: detection of different milk species by casein proteotypic peptides.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Diagnostic techniques applied to the field of cultural heritage represent a very important aspect of scientific investigation. Recently, proteomic approaches based on mass spectrometry coupled with traditional spectroscopic methods have been used for painting analysis, generating promising results for binder's protein identification. In the present work, an improved procedure based on LC-ESI/Q-q-TOF tandem mass spectrometry for the identification of protein binders has been developed for the molecular characterization of samples from an early-twentieth-century mural painting from the St. Dimitar Cathedral in Vidin, Bul...
Source: Analytical and Bioanalytical Chemistry - November 7, 2009 Category: Chemistry Authors: Chambery A, Di Maro A, Sanges C, Severino V, Tarantino M, Lamberti A, Parente A, Arcari P Tags: Anal Bioanal Chem Source Type: journals

Current activities of ISO TC229/WG2 on purity evaluation and quality assurance standards for carbon nanotubes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The current standardization activities of ISO (International Organization for Standardization) TC229 on "Nanotechnology" are introduced with focus on the work of WG2 (Working Group 2) for "measurement and characterization". Seven project groups of WG2 are actively preparing standard protocols (technical specifications) for characterization of single-wall carbon nanotubes (SWCNTs) by measurement methods such as TEM (transmission electron microscopy), SEM (scanning electron microscopy), EDX (energy-dispersive X-ray analysis), UV-Vis-NIR (ultraviolet-visible-near infrared) absorption spectroscopy, NIR-photoluminescence sp...
Source: Analytical and Bioanalytical Chemistry - November 6, 2009 Category: Chemistry Authors: Ichimura S Tags: Anal Bioanal Chem Source Type: journals

Determination of accessible amino groups on surfaces by chemical derivatization with 3,5-bis(trifluoromethyl)phenyl isothiocyanate and XPS/NEXAFS analysis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, three different types of amino-functionalized model surfaces, amino thiolate on Au, amino siloxane on Si, and polyethylene (PE) foils and films reacted with 1,2-diaminoethane (DAE) were derivatized with 3,5-bis(trifluoromethyl)phenyl isothiocyanate. Subsequently, these samples were analyzed by chemical derivatization X-ray photoelectron spectroscopy (CD-XPS) and near-edge X-ray absorption fine structure spectroscopy (NEXAFS). The determination of amino groups by this analytical approach allows gaining insight into the availability of groups on surfaces that can actually serve as attachment sites for biomolec...
Source: Analytical and Bioanalytical Chemistry - November 5, 2009 Category: Chemistry Authors: Graf N, Lippitz A, Gross T, Pippig F, Holländer A, Unger WE Tags: Anal Bioanal Chem Source Type: journals

Diamond-related materials as potential new media in separation science.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recent progress in the development of various diamond-related materials (DRMs) has induced a strong interest in their use as a stationary phase in various separation techniques. DRMs meet many requirements for use as a stationary phase in chromatography, including excellent mechanical and chemical stability, high thermal stability, low chemical reactivity of the surface, and biocompatibility. The general physicochemical properties of diamond and the preparation of different types of DRMs are reviewed, and an overview is provided of current and possible future applications in solid-phase extraction and various separatio...
Source: Analytical and Bioanalytical Chemistry - November 1, 2009 Category: Chemistry Authors: Nesterenko PN, Haddad PR Tags: Anal Bioanal Chem Source Type: journals

Time course of expression of the retinoid X receptor gene and induction of imposex in the rock shell, Thais clavigera, exposed to triphenyltin chloride.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To examine the role of the retinoid X receptor (RXR) in the development of imposex in gastropods, we investigated the time course of expression of the RXR gene in various tissues (ctenidium, ovary or testis, digestive gland, penis-forming area or penis, and head ganglia) of female and male rock shells (Thais clavigera) exposed to triphenyltin (TPT) in a flow-through exposure system for 3 months. Accumulations of TPT in tissues were clearly observed in exposed individuals, whereas no accumulation of TPT was observed in the control groups. In females, 3-month exposure to TPT resulted in the development of imposex, and pe...
Source: Analytical and Bioanalytical Chemistry - October 31, 2009 Category: Chemistry Authors: Horiguchi T, Nishikawa T, Ohta Y, Shiraishi H, Morita M Tags: Anal Bioanal Chem Source Type: journals

Fast screening immunoassay of sulfonamides in commercial fish samples.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed in plate to detect three sulfonamide residues (sulfamerazine (SMR), sulfadimetoxine (SDM), and sulfadiazine (SDZ)) in gilthead sea bream (Sparus aurata) samples. Different extraction methodologies-using methanol/water 1:1 (v/v) + ethylene diamine tetraacetic acid (EDTA) 0.5% (m/v), acetonitrile, phosphate-buffered saline (PBS) 10 mmol L(-1) pH 7 and acetate buffer 100 mmol L(-1) pH 5-and cleanup steps, based on solid-phase extraction (C(18), SCX, Si) or liquid extraction with hexane, were assayed. As optimum, a fast and simple method using ...
Source: Analytical and Bioanalytical Chemistry - October 31, 2009 Category: Chemistry Authors: Cháfer-Pericás C, Maquieira A, Puchades R, Miralles J, Moreno A Tags: Anal Bioanal Chem Source Type: journals

Analytical characterization of polymers used in conservation and restoration by ATR-FTIR spectroscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the last few decades many new polymers have been synthesized that are now being used in cultural heritage conservation. The physical and chemical properties and the long-term behaviors of these new polymers are determined by the chemical composition of the starting materials used in their synthesis along with the nature of the substances added to facilitate their production. The practical applications of these polymers depend on their composition and form (foam, film, sheets, pressure-sensitive adhesives, heat-seal adhesives, etc.). Some materials are used in restoration works and others for the exhibition, storage ...
Source: Analytical and Bioanalytical Chemistry - October 31, 2009 Category: Chemistry Authors: Chércoles Asensio R, San Andrés Moya M, de la Roja JM, Gómez M Tags: Anal Bioanal Chem Source Type: journals

Separation of peptides by HPLC using a surface-confined ionic liquid stationary phase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A butylimidazolium bromide surface-confined ionic liquid stationary phase was synthesized in-house. The synthesized phase was investigated for the separation of five peptides (Gly-Tyr, Val-Tyr-Val, leucine enkephalin, methionine enkephalin, and angiotensin-II). The peptides were successfully separated in less than 5 min. The effect of trifluoroacetic acid (TFA) on the separation of peptides was evaluated with results confirming that TFA was not acting as ion-pairing agent in separation of peptides on this phase. PMID: 19876619 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - October 31, 2009 Category: Chemistry Authors: Chitta KR, Van Meter DS, Stalcup AM Tags: Anal Bioanal Chem Source Type: journals

New approaches in GMO detection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The steady rate of development and diffusion of genetically modified plants and their increasing diversification of characteristics, genes and genetic control elements poses a challenge in analysis of genetically modified organisms (GMOs). It is expected that in the near future the picture will be even more complex. Traditional approaches, mostly based on the sequential detection of one target at a time, or on a limited multiplexing, allowing only a few targets to be analysed at once, no longer meet the testing requirements. Along with new analytical technologies, new approaches for the detection of GMOs authorized for...
Source: Analytical and Bioanalytical Chemistry - October 30, 2009 Category: Chemistry Authors: Querci M, Van den Bulcke M, Zel J, Van den Eede G, Broll H Tags: Anal Bioanal Chem Source Type: journals

The Analytical Sciences Digital Library (ASDL).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19876620 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - October 30, 2009 Category: Chemistry Authors: Larive CK Tags: Anal Bioanal Chem Source Type: journals

Development of tip-enhanced optical spectroscopy for biological applications: a review.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Tip-enhanced optical spectroscopy is an approach that holds a good deal of promise for the nanoscale characterisation of matter. Tip-enhanced Raman spectroscopy (TERS) has been demonstrated on a variety of samples: inorganic, organic and biological. Imaging using TERS has been shown for carbon nanotubes due to their high scattering efficiency. There are a number of compelling motivations to consider alternative approaches for biological samples; most importantly, the potential for heat damage of biomolecules and long acquisition times. These issues may be addressed through the development of tip-enhanced coherent anti-...
Source: Analytical and Bioanalytical Chemistry - October 30, 2009 Category: Chemistry Authors: Elfick AP, Downes AR, Mouras R Tags: Anal Bioanal Chem Source Type: journals

Dynamic non-equilibrium SPME combined with GC, PICI, and ion trap MS for determination of organophosphate esters in air.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Methodology for time-weighted average (TWA) air measurements of semivolatile organophosphate triesters, widely used flame-retardants and plasticizers, and common indoor pollutants is presented. Dynamic non-equilibrium solid-phase microextraction (SPME) for air sampling, in combination with GC/PICI and ion trap tandem MS, yields a fast, almost solvent-free method with low detection limits. Methanol was used as reagent gas for PICI, yielding stable protonated molecules and few fragments. A field sampler, in which a pumped airflow over three polydimethylsiloxane (PDMS) 100-mum fibers in series was applied, was constructed...
Source: Analytical and Bioanalytical Chemistry - October 30, 2009 Category: Chemistry Authors: Tollbäck J, Isetun S, Colmsjö A, Nilsson U Tags: Anal Bioanal Chem Source Type: journals

Erratum to: Antibiotics in food: Legislation and validation of analytical methodologies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19876623 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - October 30, 2009 Category: Chemistry Authors: Companyó R, Granados M, Guiteras J, Prat MD Tags: Anal Bioanal Chem Source Type: journals