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Association between sexual precocity and alleles of KISS-1 and GPR54 genes in goats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
KISS-1 and GPR54 were regarded as key regulators for the puberty onset and fundamental gatekeepers of sexual maturation in mammals. To explore the possible association between variations in KISS-1 and GPR54 with sexual precocity, mutation screening of exon 1 of KISS-1 and exon 1, exon 3, and partial exon 5 of GPR54 was performed in a sexual precocious breed (Jining Grey goats) and sexual late-maturing breeds (Inner Mongolia Cashmere, Angora, and Boer goats) by PCR-SSCP. The results showed that five novel mutations were identified in exon 1 and partial exon 5 of GPR54 including C96 T, T173C, G176A, G825A, and C981 T. Th...
Source: Animal Biotechnology - June 28, 2009 Category: Biotechnology Authors: Feng T, Zhao YZ, Chu MX, Zhang YJ, Fang L, Di R, Cao GL, Li N Tags: Anim Biotechnol Source Type: journals

Molecular cloning and characterization of cDNA encoding buffalo (Bubalus bubalis) cytochrome P450 aromatase in the ovary.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
One of the predominant causes of poor reproduction in buffaloes is low levels of ovarian estrogens. A rate limiting enzyme in estrogen biosynthesis is cytochrome P450 aromatase (P450 AROM), the product of CYP19 gene. In the present study CYP19 cDNA was cloned and its 5'UTR was characterized by 5'RACE in granulosa cells of large follicles. CYP19 transcripts with four different 5'UTRs (206, 114, 90 and 3 bases) were found in buffalo granulosa cells of large ovarian follicles. Interestingly, a predominant aromatase transcript with short 5'UTR (3 nucleotides) was found. Further studies are required to understand the releva...
Source: Animal Biotechnology - June 28, 2009 Category: Biotechnology Authors: Kumar OS, Rao GJ, Singh D, Sharma MK Tags: Anim Biotechnol Source Type: journals

New single nucleotide polymorphisms in the mu-calpain gene in Spanish maternal beef breeds.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Calpains play an important role in the postmortem tenderization process of meat and several SNP in the mu-calpain gene (CAPN1) have been reported to be associated with tenderness in beef cattle. Our objectives were to identify the previously reported CAPN1 331G>C SNP and to detect new polymorphisms in this gene in Spanish maternal beef breeds. A fragment (exon 8 to 10) of the bovine CAPN1 gene was sequenced and genotyped in a sample of the main Spanish maternal beef breeds including Retinta, Morucha, and Avilenã Negra-Ibérica. These breeds are characterized for their high meat quality, their adaptation to ...
Source: Animal Biotechnology - June 28, 2009 Category: Biotechnology Authors: Avilés C, Azor PJ, Pannier L, Hamill RM, Membrillo A, Molina A Tags: Anim Biotechnol Source Type: journals

Identification of novel allelic variants of integrin beta 2 (ITGB2) gene and screening for Bubaline leukocyte adhesion deficiency syndrome in indian water buffaloes (Bubalus bubalis).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A fragment of 570 bp corresponding to exon 5 and 6 of integrin beta 2 (ITGB2) gene was amplified for screening D128G mutation in one hundred and fifty two buffaloes (Bubalus bubalis) which causes bovine leukocyte adhesion deficiency syndrome (BLAD) in cattle, as well as to ascertain polymorphism. TaqI PCR-RFLP revealed no such mutation thus indicating the absence of bubaline leukocyte adhesion deficiency (BuLAD) allele in animals under study. However, the polymorphism studies using MspI restriction enzyme revealed two genotypic patterns viz. AA pattern (bands of 293, 141, 105, and 31 bp) and BB pattern (bands of 293, 1...
Source: Animal Biotechnology - June 28, 2009 Category: Biotechnology Authors: Sharma D, Kumar S, Deb SM, Mitra A, Niranjan SK, Naskar S, Sharma A Tags: Anim Biotechnol Source Type: journals

Variable levels of 37-kDa/67-kDa laminin receptor (RPSA) mRNA in ovine tissues: potential contribution to the regulatory processes of PrPSc propagation?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study presents a detailed investigation of the LRP/LR mRNA expression levels in eleven normal ovine tissues. Using real-time quantitative PCR, the highest LRP/LR expression was found in neocortex (p < 0.05). Slightly lower levels were found in the heart and obex. Intermediate levels were seen in hippocampus, cerebellum, spleen, thalamus, mesenteric lymph node, and the lowest levels were present in liver, kidney, and lung. In general, the LRP/LR mRNA levels were much higher in neuronal tissues than in peripheral tissues. The observation that differences in LRP/LR mRNA expression levels are consistent with the corres...
Source: Animal Biotechnology - June 28, 2009 Category: Biotechnology Authors: Qiao JW, Su XO, Li YX, Yang JM, Wang YQ, Kouadir M, Zhou XM, Yang LF, Yin XM, Zhao DM Tags: Anim Biotechnol Source Type: journals

Somatostatin can alter fertility genes expression, oocytes maturation, and embryo development in cattle.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to investigate the effect of somatostatin on oocytes maturation and subsequent embryo development in cattle. Bovine granulosa cells separated from oocytes, cultured for 24 h and transfected with pEGFP-N1 vector with mouse SST gene (Experimental) and with out plasmid transfection (Control). RT-PCR and Real-Time PCR were used to estimate the expression of bovine receptors of androgen, estrogen beta, growth hormone, and follicular stimulating hormone. Culture media concentrations of hormones were measured by kits using radioimmunoassay. COCs aspirated from ovaries were co-cultured with granulosa cells ...
Source: Animal Biotechnology - June 28, 2009 Category: Biotechnology Authors: Moaeen-ud-Din M, Malik N, Yang LG Tags: Anim Biotechnol Source Type: journals

Cloning and spatio-temporal expression of porcine CDK5 and CDK5R1(p35) genes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cyclin-dependent kinase 5 (CDK5) is a serine/threonine kinase homologue attributed to the mitotic cyclin-dependent kinase family. Both the kinase activity and the biological effects of CDK5 in central nervous system are mainly dependent on association with its regulatory subunit 1 known as CDK5R1 (p35). In the present study, the full-length coding regions of CDK5 and CDK5R1 were cloned from pigs. Radiation hybrid mapping localized porcine CDK5 to chromosome 18q12-13, whereas CDK5R1 was electro-localized to chromosome 12q12. Real-time quantitative RT-PCR (qRT-PCR) showed that CDK5 mRNA is ubiquitously present in all por...
Source: Animal Biotechnology - June 28, 2009 Category: Biotechnology Authors: Long H, Zhao S, Lei T, Han J, Yuan J, Qi Y, Yang Z Tags: Anim Biotechnol Source Type: journals

PLGA microsphere-mediated growth hormone release hormone expression induces intergenerational growth.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study confirms that intramuscular expression of GHRH mediated by PLGA microspheres significantly enhances intergenerational growth. PMID: 19544208 [PubMed - in process]
Source: Animal Biotechnology - June 28, 2009 Category: Biotechnology Authors: Ren XH, Zhang YL, Luo HY, Li HY, Liu SC, Zhang MJ, Ouyang SY, Xi QY, Jiang QY Tags: Anim Biotechnol Source Type: journals

Nucleotide sequence and association analysis of pig apolipoprotein-B and LDL-receptor genes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Three genes are the major determinants of heritable hypercholesterolemia diseases in humans: APOB, LDLR and LDLRAP1, which encode for proteins that physically interact to promote cholesterol uptake in the cell. We have carried out association analyses of these variants with serum cholesterol and triglycerides concentrations in a half-sib Duroc pig population. Given the structure of the population (six paternal half-sib families), we have used a statistical model that considers separately the allele transmission through dams (at population level) and through sires (within-families from heterozygous sire). Only polymorph...
Source: Animal Biotechnology - June 28, 2009 Category: Biotechnology Authors: Pena RN, Cánovas A, Varona L, Díaz I, Gallardo D, Ramírez O, Noguera JL, Quintanilla R Tags: Anim Biotechnol Source Type: journals

Exploratory study on the transcriptional profile of pigs subclinically infected with porcine circovirus type 2.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this exploratory work was to use a microarray-based approach to study the global transcriptome profile of caesarean-derived, colostrum-deprived (CDCD) piglets experimentally infected with porcine circovirus type 2 (PCV2). PCV2-inoculated piglets developed a subclinical infection, as confirmed by serology, in situ hybridization and quantitative PCR. Total RNA from mesenteric lymph nodes and lungs was obtained by duplicate from 2 control and 2 PCV2-inoculated piglets and was hybridized to Affymetrix Porcine GeneChip. Among the 24,123 probesets studied, 25 and 33 were found to be significantly differentially ex...
Source: Animal Biotechnology - June 28, 2009 Category: Biotechnology Authors: Fernandes LT, Tomás A, Bensaid A, Pérez-Enciso M, Sibila M, Sánchez A, Segalés J Tags: Anim Biotechnol Source Type: journals

Toll-like receptor 2 gene polymorphism and its relationship with SCS in dairy cattle.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, bovine TLR2 gene was taken as a candidate gene for mastitis resistance. Through PCR-SSCP analysis and sequencing, three missense mutations at T385 G, G398A, and G1884A were detected in the coding region that encoded extracellular domain. Altogether 240 dairy cattle of three breeds (Holstein, Simmental, and Sanhe cattle) were genotyped and allele frequencies were determined. The effects of TLR2 polymorphisms on somatic cell score (SCS) were analyzed and significant association was found between T385 G and SCS. The mean of genotype GG was significantly lower than those of genotype TT and TG. No significant ass...
Source: Animal Biotechnology - June 28, 2009 Category: Biotechnology Authors: Zhang LP, Gan QF, Ma TH, Li HD, Wang XP, Li JY, Gao X, Chen JB, Ren HY, Xu SZ Tags: Anim Biotechnol Source Type: journals

Lingual antimicrobial peptide expresses in buffalo mammary gland.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study envisages role of beta-defensin peptides in innate immunity of mammary gland. PMID: 19370457 [PubMed - in process]
Source: Animal Biotechnology - June 12, 2009 Category: Biotechnology Authors: Das H, Ahmed SU, Thakuria D, Pathan MM, Latif A, Roy SK, More T Tags: Anim Biotechnol Source Type: journals

Associations of a HinfI PCR-RFLP of POU1F1 gene with growth traits in Qinchuan cattle.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objectives of the present study were to estimate the allele and genotype frequencies of the POU1F1/HinfI polymorphisms in beef cattle belonging to four different genetic groups and to determine the effects of these polymorphisms on growth traits in cattle. The 451-bp PCR products of POU1F1 gene digested with HinfI exhibited three genotypes and two alleles, which were at Hardy-Weinberg equilibrium (P > 0.05). Genotype BB was the predominant genotype and B the predominant allele in the studied populations. There was significant difference between Limousin x Qinchuan and Qinchuan in the distribution of genotypes (P <...
Source: Animal Biotechnology - June 12, 2009 Category: Biotechnology Authors: Zhang C, Liu B, Chen H, Lan X, Lei C, Zhang Z, Zhang R Tags: Anim Biotechnol Source Type: journals

The fat mass and obesity-associated (FTO) gene is associated with intramuscular fat content and growth rate in the pig.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The association of the FTO gene with obesity has been implicated in various human populations. The FTO gene is also most likely involved in the regulation of energy balance and feed intake. Here, the FTO gene was studied as a candidate gene for fatness and growth rate traits in pigs. The amino acid sequence of the FTO gene showed high conservation among human, pig, and other important domestic animals. Twelve variants including ten SNPs and two indels were detected, and then five SNPs within different genomic regions were genotyped in the ISU Berkshire x Yorkshire pig resource family. The linkage disequilibrium analyse...
Source: Animal Biotechnology - June 12, 2009 Category: Biotechnology Authors: Fan B, Du ZQ, Rothschild MF Tags: Anim Biotechnol Source Type: journals

Microsatellite analysis characterizes Burkina Faso as a genetic contact zone between Sahelian and Djallonke sheep.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A total of 123 sheep belonging to the Djallonke, Mossi, and Burkina-Sahel breeds, along with 41 Spanish Xalda sheep were genotyped for 27 microsatellites. The pair Djallonke-Mossi had the highest between breeds molecular coancestry. Admixture analysis informed on the parental role of the Burkina-Sahel and Djallonke breeds. The Mossi breed was a hybrid population nearer to the Djallonke breed. Only half of the Mossi individuals were correctly assigned to their breed. The Burkina-Sahel and Djallonke breeds can be considered ancestrally different genetic entities. Differentiation between the Djallonke and Mossi breeds may...
Source: Animal Biotechnology - June 12, 2009 Category: Biotechnology Authors: Alvarez I, Traore A, Tamboura HH, Kabore A, Royo LJ, Fernandez I, Ouedraogo-Sanou G, Sawadogo L, Goyache F Tags: Anim Biotechnol Source Type: journals

Characterization of exon 2 and intron 2 of leptin gene in Indian goats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Leptin, the hormonal product of the leptin (obese) gene (LEP), has multiple physiological effects and plays a pivotal role in the control of body growth, immune function, and reproduction. LEP gene polymorphism has been analyzed in cattle, buffalo, and pigs, and the polymorphic pattern has been associated with energy balance, milk production, and live weight and fertility trait in different livestock species. The present study has been designed to analyze polymorphism in exon 2 and intron 2 region of leptin gene in Indian goats. Genotyping was carried out in 111 kids including 70 samples from Barbari and 41 samples fro...
Source: Animal Biotechnology - June 12, 2009 Category: Biotechnology Authors: Singh SK, Rout PK, Agarwal R, Mandal A, Singh SK, Shukla SN, Roy R Tags: Anim Biotechnol Source Type: journals

Molecular Cloning and Expression Analysis of CD127 in Pig.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the full-length cDNA of pig Cd127 was cloned and sequenced. Pig CD127 cDNA contains an open reading frame (1,380 bp) encoding 459 amino acids. Phylogenetic analysis revealed that pig CD127 is most homologous to that of cow (Bos taurus) with 87% amino acid identity and less homologous to that of chicken (Gallus gallus) with 36% amino acid identity among those of species. Structurally, pig CD127 has well-conserved cytoplasmic tail that transmits a signal and little diversity of extracellular domain, compared with those of other mammalian species. Expression analysis indicates that the mRNA transcript of pig CD...
Source: Animal Biotechnology - January 23, 2009 Category: Biotechnology Authors: Hur YJ, Kim JG, Whang KY, Kim YB Tags: Anim Biotechnol Source Type: journals

Porcine Type III RNA Polymerase III Promoters for Short Hairpin RNA Expression.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Based on the highly conserved sequences of small nuclear RNA and small cytoplasmic RNA between vertebrate species, three porcine type III RNA polymerase III promoters, pY1, pY3 and pU6, were identified by using genomic DNA walking. To test the functional relationship of these sequences, the human H1 promoter of pSUPER-EGFP-l-neo vector was substituted with these three promoters to create the ppPol III-MCS vectors. The strength of each promoter was measured by its ability to derive expression of shRNA to repress expression of luciferase via RNA interference in the pig kidney epithelial cell line LLC-PK1. We determine th...
Source: Animal Biotechnology - January 23, 2009 Category: Biotechnology Authors: Chuang CK, Lee KH, Fan CT, Su YS Tags: Anim Biotechnol Source Type: journals

Genomic Location of the Bovine Growth Hormone Secretagogue Receptor (GHSR) Gene and Investigation of Genetic Polymorphism.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the bovine GHSR gene was mapped to BTA1 between BL26 and BMS4004. Two different bovine GHSR CDS (GHSR1a and GHSR1b) were sequenced. Six polymorphisms (five SNPs and one 3-bp indel) were also identified, three of them leading to amino acid variations L24V, D194N, and Del R242. These variations are located in the extracellular N-terminal end, the exoloop 2, and the cytoloop 3 of the receptor, respectively. PMID: 19160085 [PubMed - in process]
Source: Animal Biotechnology - January 23, 2009 Category: Biotechnology Authors: Colinet FG, Vanderick S, Charloteaux B, Eggen A, Gengler N, Renaville B, Brasseur R, Portetelle D, Renaville R Tags: Anim Biotechnol Source Type: journals

Targeted nucleotide exchange in bovine myostatin gene.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this work was to implant the polymorphism G938A, naturally existent in Piedmontese breed, into in vitro propagated foetal myoblasts, from Nellore cattle. Single strand DNA (ssDNA) oligonucleotides were used to direct the same nucleotidic transition (G938A) to exon 3. Two transfection protocols (cationic lipid solution and electroporation) were tested and, 48 hours after transfection, RNA and DNA were extracted from myoblasts. Reverse transcription and polymerase chain reaction (PCR) were performed, using primers flanking the mutation region. The PCR products were cloned and analyzed by DNA sequencing, and ...
Source: Animal Biotechnology - January 23, 2009 Category: Biotechnology Authors: Grisolia AB, Curi RA, De Lima VF, Olmedo HP, Kmiec E, Nunes CM, Aoki SM, Garcia JF Tags: Anim Biotechnol Source Type: journals

Enhanced Host Immune Recognition of Mastitis Causing Escherchia Coli in CD-14 Transgenic Mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Escherchia coli causes mastitis, an economically significant disease in dairy animals. E. coli endotoxin (lipopolysaccharide, LPS) when bound by host membrane proteins such as CD-14, causes release of proinflammatory cytokines recruiting neutrophils as an early, innate immune response. Excessive proinflammatory cytokines causes tissue damage, compromising mammary function. If present, soluble CD-14 (sCD-14) might out compete membrane bound CD-14, lessening the severity of the inflammatory response. To test this hypothesis transgenic mice, expressing sCD-14 in their milk (31 to 316 mug/ml), were evaluated. A cell cultur...
Source: Animal Biotechnology - January 23, 2009 Category: Biotechnology Authors: Wall R, Powell A, Sohn E, Foster-Frey J, Bannerman D, Paape M Tags: Anim Biotechnol Source Type: journals

Association and Haplotype Analysis of purH Gene with Inosine Monophosphate Content in Chickens.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The current study was designed to investigate the effects of the purH gene on chicken muscle inosine monophosphate (IMP) content. Muscle IMP content was measured in five chicken breeds. Single nucleotide polymorphisms (SNPs) were detected by PCR-SSCP and DNA sequencing. Two SNPs were detected, A/T substitution at position 8023 in exon 9, and T/C substitution at position 17446 in exon 16. The results indicated that only T17446C polymorphism was associated with IMP content. The haplotype effect was higher than the single genotype effect. We tentatively conclude that purH gene is a candidate locus or linked to a major gen...
Source: Animal Biotechnology - October 16, 2008 Category: Biotechnology Authors: Shu JT, Bao WB, Zhang XY, Zhang HX, Chen GH Tags: Anim Biotechnol Source Type: journals

Simple sequence repeats for genetic studies of alpaca.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This sequence set contained 2,290 sequences of length > 100 nucleotides that contained microsatellites of length >/= 14 dinucleotide or 10 trinucleotide repeats with purity equal to 100%. An additional 13 sequences contained a GC microsatellite of length >/= 12 repeats (purity = 100%) were also obtaine...
Source: Animal Biotechnology - October 16, 2008 Category: Biotechnology Authors: Reed KM, Chaves LD Tags: Anim Biotechnol Source Type: journals

Molecular Cloning and Expression Analysis of CD82 in Pig.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the full-length cDNA of pig CD82 was cloned and sequenced. Pig Cd82 cDNA contains an open reading frame (801 bp) encoding 266 amino acids. Sequence alignment results indicated that pig CD82 cDNA evidenced 85.45%, 85.63%, 77.03%, and 77.78% identity with human, cattle, rat, and mouse, respectively. In the expression study, the constitutive expression of swine Cd82 mRNA was detected in a variety of tissues, including lymphoid tissues as well as nonlymphoid tissues. Future studies will be focused on the functional role of CD82 during the course of pig infectious diseases or tumor development. PMID: 18855249...
Source: Animal Biotechnology - October 16, 2008 Category: Biotechnology Authors: Cho KW, Kim JG, Whang KY, Jung WW, Kim YB Tags: Anim Biotechnol Source Type: journals

Evaluation of the Titin-Cap Gene (TCAP) as Candidate for Dilated Cardiomyopathy in Irish Wolfhounds.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Dilated cardiomyopathy (DCM) is a myocardial disorder characterized by left ventricular dilatation and impaired systolic contraction. Irish wolfhounds (IW) and other large breed dogs are most commonly disposed to DCM. We analyzed the titin-cap (TCAP, telethonin) gene as candidate for DCM. Genomic DNA was analyzed in eight DCM affected and five DCM-free IWs. cDNA was sequenced in one DCM-affected IW and two unaffected dogs, one Tibetan terrier and one Dachshund. Compared to the Boxer reference sequence, one sequence difference was identified in the 3'UTR and two in the intron sequence. In the IWs the sequences were mono...
Source: Animal Biotechnology - October 16, 2008 Category: Biotechnology Authors: Philipp U, Vollmar A, Distl O Tags: Anim Biotechnol Source Type: journals

Detection of Bovine Central Nervous System Tissue as Bovine Spongiform Encephalopathy Risk Material by Real-Time Reverse Transcriptase-PCR in Raw and Cooked Beef Products.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
There is increasing evidence of the association of the new variant of Creutzfeldt-Jacob disease (nvCJD) in humans with bovine spongiform encephalopathy (BSE) in cattle. Many countries established legislation of banning central nervous system (CNS) tissues, which are regarded as BSE-specified risk materials (SRM), in human food supply because of the potential transmission of BSE to humans. A real-time reverse transcriptase-PCR assay using the bovine glial fibrillary acidic protein (GFAP) mRNA template for the detection of CNS tissues in raw and cooked beef products was developed in this study. The results showed that (1...
Source: Animal Biotechnology - October 16, 2008 Category: Biotechnology Authors: Shi XJ, Ma GP, Li BL, Yang JL, Li YX, Liu XH, Liu QG Tags: Anim Biotechnol Source Type: journals

Trichostatin A Improved Epigenetic Modifications of Transfected Cells but did not Improve Subsequent Cloned Embryo Development.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, treatment of transfected cells with TSA decreased the genome DNA methylation level, increased histone acetylation, and eGFP gene expression was activated. Donor cells with reduced DNA methylation did not improve subsequent cloned embryo development; however, transgene expression was improved in cloned embryos. PMID: 18855246 [PubMed - in process]
Source: Animal Biotechnology - October 16, 2008 Category: Biotechnology Authors: Wu X, Li Y, Li GP, Yang D, Yue Y, Wang L, Li K, Xin P, Bou S, Yu H Tags: Anim Biotechnol Source Type: journals

Functional characterization of the chicken Peptide transporter 1 (pept1, slc15a1) gene.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study analyzed the promoter region of the cPepT1 gene. Using deletion analysis, positive-acting (- 314 to - 261, - 169 to - 155, and - 120 to - 60) and negative-acting (- 419 to - 386 and - 214 to - 169) regions were mapped in transfected chick embryo fibroblasts (CEF). The addition of neither amino acids Phe, Arg, or Val, nor the dipeptides Gly-Sar (glycyl-sarcosine), Gly-Pro, Gly-Phe, Met-Pro, Met-Lys or Lys-Lys, had an effect on cPepT1 promoter activity in transfected CEF. The cPepT1 promoter was more active in CEF and primary chicken intestinal cells than in chicken liver cells. This study represents a functional ...
Source: Animal Biotechnology - October 16, 2008 Category: Biotechnology Authors: Frazier S, Ajiboye K, Olds A, Wyatt T, Luetkemeier ES, Wong EA Tags: Anim Biotechnol Source Type: journals

Sequence Variation Of the alpha-Lactalbumin Gene In Holstein and Nellore Cows.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to analyze allelic frequency differences at position - 1689 (g. A > G) and + 15 (g. A > G) of the alpha-lactalbumin gene in Holstein (Bos taurus) and Nellore (Bos indicus) cows. Blood samples were analyzed from 34 Holstein, 104 Nellore, and 99 Dairy Nellore cows using PCR-RFLP. The different RFLP patterns were sequenced and a novel sequence variation on nucleotide - 46 was identified. An adenine at this position was designated as the A allele and a guanine was designated B allele. The frequencies of alleles A - 1689, A - 46, and A + 15 differed between Holstein and both Nellore breeds. The results sh...
Source: Animal Biotechnology - July 9, 2008 Category: Biotechnology Authors: Martins LF, Milazzotto MP, Feitosa WB, Coutinho AR, Simoes R, Marques MG, Assumpcao ME, Visintin JA Tags: Anim Biotechnol Source Type: journals

Gene expression of heart- and adipocyte-Fatty Acid-binding protein and correlation with intramuscular fat in chinese chickens.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study examined the association between expression of heart- and adipocyte-fatty acid binding-protein genes (H- and A-FABP) with intramuscular fat percentage (IFP) in two Chinese chicken breeds (Beijingyou [BJY] and Jingxing [JX]). The results showed that age and breed had significant effects on the FABP expression. A-FABP mRNA levels were dramatically higher in BJY than in JX chickens and in males than in females. The results indicate that transcription of H- and A-FABP genes was significantly correlated with IFP in two breeds of chicken. PMID: 18607791 [PubMed - in process]
Source: Animal Biotechnology - July 9, 2008 Category: Biotechnology Authors: Li WJ, Li HB, Chen JL, Zhao GP, Zheng MQ, Wen J Tags: Anim Biotechnol Source Type: journals

Genomic organization and promoter analysis of the bovine adam12 gene.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we have determined the genomic structure of 5'- and 3'-regions in the bovine ADAM12 gene. We could obtain characteristics of lower homology of its exon 2 with human counterpart. Human exon S19 encodes for the sequence specific to a shorter secreted form of ADAM12S. The bovine ADAM12 gene had no canonical 3'-splice acceptor site at 5'-side of the putative exon S19, suggesting that the cattle could not produce a ADAM12S counterpart. To identify the regulatory elements, a 12 kb 5'-flanking region of the gene was cloned and luciferase reporter assay was carried out. Reporter plasmids with different length of pro...
Source: Animal Biotechnology - July 9, 2008 Category: Biotechnology Authors: Taniguchi Y, Doronbekov K, Yamada T, Sasaki Y, Takano A, Sugimoto Y Tags: Anim Biotechnol Source Type: journals

Using mtDNA Sequences to Estimate SNP Parameters in ESTs.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study uses sequences from four turkey muscle cDNA libraries to demonstrate how mtDNA sequences gleaned from collections of ESTs can be used to estimate SNP parameters and thus help predict the validity of SNPs. PMID: 18607789 [PubMed - in process]
Source: Animal Biotechnology - July 9, 2008 Category: Biotechnology Authors: Reed KM Tags: Anim Biotechnol Source Type: journals

Molecular characterization and SNP development for the porcine il6 and il10 genes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Different cytokines are secreted in response to specific microbial molecules referred to as pathogen associated molecular patterns (PAMPs). Interleukin 6 (IL6) and interleukin 10 (IL10), both secreted by macrophages and lymphocytes, play a central role in the immunological response. In this work we obtained the genomic structure and complete DNA sequence of the porcine IL6 and IL10 genes and identified polymorphisms in the genomic sequences of these genes on a panel of ten different pig breeds. Comparative intra- and interbreed sequence analysis revealed a total of eight polymorphisms in the porcine IL6 gene and 21 in ...
Source: Animal Biotechnology - July 9, 2008 Category: Biotechnology Authors: Daniłowicz E, Akouchekian M, Drogemuller C, Haase B, Leeb T, Kuiper H, Distl O, Iras FC Tags: Anim Biotechnol Source Type: journals

Sequence Identification, Tissue Distribution and Polymorphism of the Porcine Cathepsin D (CTSD) Gene.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we characterized the full-length cDNA, genomic DNA sequence, expression profile and polymorphism of the porcine CTSD gene. The full-length cDNA of porcine CTSD gene and the predicted protein sequence shared high identities wih other mammalian orthologous. Northern-blot analysis and Reverse transcription (RT)-PCR results indicated that the CTSD gene has one transcript of approximately 2.0 kb in normal tissues and was expressed ubiquitously in pigs, without significant differences in porcine heart, liver, spleen, lung, kidney, stomach, fat, triceps brachi, biceps femoris, and longissimus muscles. The porcine C...
Source: Animal Biotechnology - July 9, 2008 Category: Biotechnology Authors: Mei Y, Chen Y, Li J, Gao P, Wang C, Zhang H, Ling F, Li Y, Xie S, Li S, Zhang G Tags: Anim Biotechnol Source Type: journals

Characterization of Porcine ASB6 Gene and Transcripts-Comparison of Mammalian Transcripts.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present here a detailed analysis of ASB6 transcripts in pigs. We demonstrate the existence of an alternative transcript resulting from intron retention. This secondary transcript, if functional, encodes a protein without SOCS box. A comparison of mammalian ASB6 transcripts is performed to demonstrate the importance of transcripts encoding for a truncated ASB6 protein. PMID: 18607786 [PubMed - in process]
Source: Animal Biotechnology - July 9, 2008 Category: Biotechnology Authors: Robic A, Faraut T, Liaubet L, Riquet J, Milan D, Lobjois V Tags: Anim Biotechnol Source Type: journals

Porcine Lung Surfactant Protein B Gene (SFTPB): cDNA Sequencing, Chromosomal Location, and Expression Studies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The porcine surfactant protein B (SFTPB) is a single copy gene on chromosome 3. Three different cDNAs for the SFTPB have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the SFTPB transcript. The expression level for the SFTPB gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed si...
Source: Animal Biotechnology - July 9, 2008 Category: Biotechnology Authors: Cirera S, Fredholm M Tags: Anim Biotechnol Source Type: journals

A new tandem repeat in bovine fibrinogen Aalpha gene.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we describe intraspecies variation in the alphaC connector region of the bovine fibrinogen Aalpha gene. Sequencing and genotyping of six bovine breeds revealed 7 to 10 tandem repeats in the alphaC connector region. In addition, we observed length differences between B. indicus and B. taurus, with the B. indicus having longer fibrinogen alphaC connectors (10-repeat alleles) than B. taurus (7- and 9-repeats). The difference in tandem repeats may be related to the function of blood coagulation system. PMID: 18432403 [PubMed - indexed for MEDLINE]
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Inaba Y, Tsurifune K, Sasazaki S, Mannen H Tags: Anim Biotechnol Source Type: journals

cDNA cloning and tissue expression analyses of the encoding regions for three novel porcine genes- MJD1, CDC42 and NECD.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The cDNAs for Machado-Joseph disease protein 1 homolog (MJD1), cell division control protein 42 homolog precursor(CDC42) and necdin (NECD) genes of pig were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved coding sequence information of the MJD1, CDC42, and NECD genes from mouse and other mammals and the referenced porcine EST sequence information. Tissue expression analysis showed the swine MJD1, CDC42, and NECD genes were obviously differentially expressed in different tissues including muscle, heart, liver, backfat, kidney, lung, small intestine, and large intestine...
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Liu GY, Gao SZ, Ge CR, Zhang X Tags: Anim Biotechnol Source Type: journals

Simultaneous expression of growth hormone releasing hormone (GHRH) and hepatitis B surface antigen/somatostatin (HBsAg/SS) fusion genes in a construct in the skeletal muscle enhances rabbit weight gain.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Somatostatin (SS) and growth hormone-releasing hormone (GHRH) are synthesized and secreted by the hypothalamus, which can control the synthesis and secretion of the growth hormone (GH) from the hypophysis as well as regulate the GH concentrations in animals and humans. In this article, we describe the regulation of animal growth using plasmid DNA encoding both the GHRH gene and the SS gene fused with the hepatitis B surface antigen (HBsAg) gene. We constructed a series of expression plasmids to express the GHRH and HBsAg-SS fusion genes individually as well as collectively. The fusion gene and GHRH were successfully ex...
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Dai JW, Liu SC, Hao LL, Zhang YL, Zhang Q, Ren XH, Jiang QY Tags: Anim Biotechnol Source Type: journals

WDNM1 is associated with differentiation and apoptosis of mammary epithelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNFalpha treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNFalpha induced WDNM1 expression showed the NFkappaB-dependent mechanism since it's expression was abrogated in IkappaBalphaM (super-repressor of NFkappaB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. T...
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Kho Y, Kim S, Yoon BS, Moon JH, Kwak S, Park G, Woo J, Oh S, Hong K, Kim S, Kim H, You S, Choi Y Tags: Anim Biotechnol Source Type: journals

Development of cell lines from the sheep used to construct the CHORI-243 Ovine BAC library.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Two cell lines, designated MARC.OVSM, and MARC.OKF, were initiated from the aorta and kidney, respectively, obtained from the Texel ram used to make the CHORI-243 Ovine BAC library. These cell lines have been submitted to the NIA Aging Cell Repository at the Coriell Cell Respositories, Camden, NJ, USA, and will be made publicly available. PMID: 18432399 [PubMed - indexed for MEDLINE]
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Chitko-McKown CG, Leonard JC, Moscatello D, Miller LC, Freking BA Tags: Anim Biotechnol Source Type: journals

Association and in silico assignment of sequences from turkey BACs.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bacterial artificial chromosomes (BACs) provide an important resource in genetic mapping. An initial set of BACs corresponding to microsatellite markers in the turkey (Meleagris gallopavo) was isolated from the CHORI-260 turkey BAC library. The selected markers were distributed on both macro- and microchromosomes and included a genetically unlinked marker. End sequences were obtained for a subset of the recovered BACs and compared to the chicken whole genome sequence. Close association of the turkey BAC-end sequences and original marker sequences was generally conserved in the chicken genome. Gene content of the turkey...
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Reed KM, Faile GM, Kreuth SB, Chaves LD, Sullivan LM Tags: Anim Biotechnol Source Type: journals

A comparative study on the efficiency of two enucleation methods in pig somatic cell nuclear transfer: effects of the squeezing and the aspiration methods.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, two enucleation methods, the squeezing and the aspiration methods, were compared. The efficiency of these two methods to enucleate pig oocytes and the in vitro and in vivo viability of somatic cell nuclear transfer (SCNT) pig embryos, were evaluated. In the squeezing method, the zona pellucida was partially dissected and a small amount of cytoplasm containing metaphase II (MII) chromosomes and the first polar body (PB) were pushed out. In the aspiration method, the PB and MII chromosomes were aspirated using a beveled micropipette. After injection of fetal fibroblasts into the perivitelline space, reconstruc...
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Lee E, Estrada J, Piedrahita JA Tags: Anim Biotechnol Source Type: journals

SNiPORK - a microarray of SNPs in candidate genes potentially associated with pork yield and quality - development and validation in commercial breeds.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
SNiPORK is an oligonucleotide microarray based on the arrayed primer extension (APEX) technique, allowing genotyping of single nucleotide polymorphisms (SNPs) in genes of interest for pork yield and quality traits. APEX consists of a sequencing reaction primed by an oligonucleotide anchored with its 5' end to a glass slide and terminating one nucleotide before the polymorphic site. Extension with one fluorescently labeled dideoxynucleotide complementary to the template reveals the polymorphism. Ninety SNPs were selected from those associated directly or potentially with pork traits. Of the 90 SNPs, 5 did not produce a ...
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Kaminski S, Help H, Brym P, Rusc A, Wójcik E Tags: Anim Biotechnol Source Type: journals

Piggy-BACing the human genome I: constructing a porcine BAC physical map through comparative genomics.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Availability of the human genome sequence and high similarity between humans and pigs at the molecular level provides an opportunity to use a comparative mapping approach to piggy-BAC the human genome. In order to advance the pig genome sequencing initiative, sequence similarity between large-scale porcine BAC-end sequences (BESs) and human genome sequence was used to construct a comparatively-anchored porcine physical map that is a first step towards sequencing the pig genome. A total of 50,300 porcine BAC clones were end-sequenced, yielding 76,906 BESs after trimming with an average read length of 538 bp. To anchor t...
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Rogatcheva MB, Chen K, Larkin DM, Meyers SN, Marron BM, He W, Schook LB, Beever JE Tags: Anim Biotechnol Source Type: journals

Genetic imprinting of H19 and IGF2 in domestic pigs (Sus scrofa).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The genes insulin-like growth factor 2 (IGF2) and H19 express paternally and maternally, respectively, in humans, mice, sheep, and cattle. Additionally, IGF2 has been shown to be regulated by at least four promoters in a tissue- or development-specific manner. In the domestic pigs, the promoter- and tissue-specific imprinting pattern of IGF2 has not been well characterized, nor is the imprinting pattern of H19. In the present study, we identified two polymorphisms in each of IGF2 (exons 2 and 9) and H19 (exons 1 and 5) and determined the imprinting status of these two genes in 13 organs / tissues of week-old pigs. IGF2...
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Li C, Bin Y, Curchoe C, Yang L, Feng D, Jiang Q, O'Neill M, Tian XC, Zhang S Tags: Anim Biotechnol Source Type: journals

Enhancement of extra chromosomal recombination in somatic cells by affecting the ratio of homologous recombination (HR) to non-homologous end joining (NHEJ).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Advancements in somatic cell gene targeting have been slow due to the finite lifespan of somatic cells and the overall inefficiency of homologous recombination. The rate of homologous recombination is determined by mechanisms of DNA repair, and by the balance between homologous recombination (HR) and non-homologous end joining (NHEJ). A plasmid-to-plasmid, extra chromosomal recombination system was used to study the effects of the manipulation of molecules involved in NHEJ (Mre11, Ku70/80, and p53) on HR/NHEJ ratios. In addition, the effect of telomerase expression, cell synchrony, and DNA nuclear delivery was examined...
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Zaunbrecher GM, Dunne PW, Mir B, Breen M, Piedrahita JA Tags: Anim Biotechnol Source Type: journals

SNPS in the promoter regions of the canine RMRP and SHOX genes are not associated with canine chondrodysplasia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Canine chondrodysplasia is a heritable defect of endochondral ossification characterized by disproportionately short limbs. It is directly linked to significant health concerns, such as intervertebral disc disease. Some human skeletal dysplasias exhibit similar disproportionate dwarfisms and are associated with mutations in the RMRP and SHOX genes. These phenotypic similarities indicated RMRP and SHOX as candidate genes in dogs. They were sequenced in three chondrodysplastic and three normal-legged breeds. Single nucleotide polymorphisms in the promoter regions of both genes and in exon 2 of SHOX were found in affected...
Source: Animal Biotechnology - July 7, 2008 Category: Biotechnology Authors: Young AE, Bannasch DL Tags: Anim Biotechnol Source Type: journals

The Atlantic Salmon MHC class II alpha and beta promoters are active in mammalian cell lines.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The major histocompatibility complex class II (MHCII) genes are only constitutively expressed in certain immune response cells such as B cells, macrophages, dendritic cells and other antigen presenting cells. This cell specific expression pattern and the presence of conserved regions such as the X-, X2-, Y-, and W-boxes make the MHCII promoters especially interesting as vector constructs. We tested whether the Atlantic salmon (Salmo salar L.) MHCII promoters can function in cell lines from other organisms. We found that the salmon MHCII alpha and MHCII beta promoters could drive expression of a LacZ reporter gene in ad...
Source: Animal Biotechnology - January 1, 2007 Category: Biotechnology Authors: Vestrheim O, Lundin M, Syed M Tags: Anim Biotechnol Source Type: journals

Evaluation of the CTSL2 gene as a candidate gene for alopecia X in Pomeranians and Keeshonden.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Alopecia X is a noninflammatory, progressive, bilateral symmetric alopecia in dogs. The disease is mainly found in Nordic breeds. The breed predisposition and a strong familial accumulation suggest a hereditary background. We analyzed the cathepsin L2 gene (CTSL2) as a candidate for alopecia X. The comparative sequencing of 14 affected and 18 control animals revealed ten polymorphisms; however, none of these polymorphisms affected the coding sequence. Haplotype analysis did not reveal an association of one particular CTSL2 haplotype with the disease phenotype; therefore, we conclude that the CTSL2 gene is probably not ...
Source: Animal Biotechnology - January 1, 2007 Category: Biotechnology Authors: Mausberg EM, Drögemüller C, Leeb T, Dolf G, Rüfenacht S, Welle M Tags: Anim Biotechnol Source Type: journals