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193 records returned

Field effect in cancer-an update.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The concept "field effect in cancer" originated in 1953 from the histopathological observations of Slaughter and colleagues [1] regarding the occurrence of multiple primary oral squamous cell carcinomas and their local recurrences. The development of modern molecular technologies has extended the field effect concept by exploring the molecular abnormalities in tissues that appear histologically normal. To date, such field effect biomarkers have been reported in several sites and organs, eg, head and neck, colon and rectum, prostate, breast, lung, esophagus, stomach, and skin. Two popular hypotheses have been proposed. ...
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Chai H, Brown RE Tags: Ann Clin Lab Sci Source Type: journals

An academic-based hospital donor site: do physicians donate blood?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study shows the dearth of physician blood donors and a strong cohort of institution-affiliated repeat donors. Physicians represent a potential, stable, and sustainable donor pool; further studies are needed to establish physician recruitment programs. PMID: 19880760 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Kanner WA, Jeffus S, Wehrli G Tags: Ann Clin Lab Sci Source Type: journals

Correlation of JAK2 V617F mutant allele quantitation with clinical presentation and type of chronic myeloproliferative neoplasm.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Activating JAK2 V617F mutation is present in many patients with chronic myeloproliferative neoplasms. We evaluated, retrospectively, clinical and laboratory data from 70 patients with BCR-ABL1 negative, JAK2 positive, chronic myeloproliferative disease. Quantity of the JAK2 mutant allele was tested for correlation with the clinical presentation, type of chronic myeloproliferative disease, hemoglobin level, white blood cell and platelet counts, spleen size, and/or cardiovascular complications. RealTime-PCR was used for amplification of DNA from marrow or peripheral blood. Polycythemia vera was more frequently diagnosed ...
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Malysz J, Crisan D Tags: Ann Clin Lab Sci Source Type: journals

A peptide from a ras effector-domain blocks ras-dependent cardiac hypertrophy in myocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PNC-2 is a peptide corresponding to an effector domain (residues 96-110) of ras-p21 that strongly and specifically blocks mitogenic signal transduction by oncogenic but not activated, normally-expressed wild-type ras-p21 protein. Since myocardial hypertrophy can be induced both by oncogenic and overexpressed wild-type ras-p21, we investigated whether PNC-2 can block norepinephrine (NE)-induced, ras-dependent myocardial hypertrophy in cardiac myocytes. Since PNC-2 blocks oncogenic ras-p21-induced activation of JNK and ERK, we further determined whether this peptide blocks activation of these kinases in NE-treated myocyt...
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Deng L, Boujdir M, Tremontano A, Pincus MR, El-Sherif N Tags: Ann Clin Lab Sci Source Type: journals

Holotranscobalamin as an indicator of vitamin B12 deficiency in gastrectomized patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Transcobalamin-bound vitamin B(12), or holotranscobalamin (HoloTC), a biologically active form, is believed to be a sensitive marker of vitamin B(12) deficiency. We investigated the prevalence of vitamin B(12) deficiency in gastrectomized patients using HoloTC and total vitamin B(12) to determine the diagnostic utility of HoloTC. We enrolled 128 gastrectomized patients and measured serum HoloTC, total vitamin B(12), homocysteine (Hcy), and complete blood count (CBC). HoloTC values were also obtained from 100 healthy controls. The precision of HoloTC measurement was good and the normal range of HoloTC was set at >or=...
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Lee YK, Kim HS, Kang HJ Tags: Ann Clin Lab Sci Source Type: journals

Thin-layer cytopathology of a gastrointestinal stromal tumor (GIST) in effusion: diagnostic dilemmas.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Although gastrointestinal stromal tumors (GISTs) are uncommon, they represent the most frequent mesenchymal neoplasms of the gastrointestinal tract. During recent years, considerable information has been published about the pathogenesis, molecular biology, histological criteria, surgery, and adjuvant pharmacological treatment of GISTs, but there have been few reports about the cytologic diagnosis of GISTs, particularly in effusions; in such specimens these neoplasms cause a wide range of potential pitfalls. In this case report, we show that by combining morphological and immunocytochemical studies on thin layer slide p...
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Zappacosta R, Caraceni D, Stura S, Zappacosta B, Rosini S Tags: Ann Clin Lab Sci Source Type: journals

Biochemical changes associated with reperfusion after off-pump and on-pump coronary artery bypass graft surgery.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A prospective study was performed to monitor the postoperative changes in biochemical markers associated with reperfusion injury following (i) cardiopulmonary bypass (CPB) with aortic cross-clamping and cardioplagia (CABG); (ii) CPB with a tissue stabilizing device (SUP.CPB); or (iii) surgery on beating heart (off-pump CABG or OPCABG). Of the 48 patients, 16 were subjected to CABG, 16 to SUP.CPB, and 16 to OPCABG. Arterial and venous blood samples drawn 10 min preoperatively and 0.2, 4, 24, and 48 hr after surgery were assayed for plasma lactate, total calcium, and ionized calcium and erythrocyte glutathione peroxidase...
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Chandrasena LG, Peiris H, Waikar HD Tags: Ann Clin Lab Sci Source Type: journals

Acetaminophen combinations protect against iron-induced cardiac damage in gerbils.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study tested if acetaminophen, N-methyl-D-glucamine dithiocarbamate (NMGDTC), deferoxamine, and combinations of these agents reduce excess iron content, prevent iron-induced pathology, reduce cardiac arrhythmias, and reduce mortality in iron-overloaded gerbils. Eight groups of 16 gerbils received iron dextran injections (ferric hydroxide dextran complex, 120 mg/kg, ip) or saline solution (controls) twice/wk for 8 wk. The 8 groups were treated every Monday, Wednesday, and Friday with one of the following: saline control, acetaminophen, 150 mg/kg, ip), acetaminophen (150 mg/kg, po), deferoxamine, 83 mg/kg, ip), NMGDTC (...
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Walker EM, Morrison RG, Dornon L, Laurino JP, Walker SM, Studeny M, Wehner PS, Rice KM, Wu M, Blough ER Tags: Ann Clin Lab Sci Source Type: journals

Effects of high methionine diet on oxidative stress in serum, apo-B containing lipoproteins, heart, and aorta in rabbits.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study investigated in rabbits whether a high methionine (HM) diet influences oxidative stress parameters in serum, apo-B containing lipoproteins (LDL+VLDL), heart, and aorta. Rabbits received a normal commercial chow supplemented with 2% L-methionine (w/w) for 6 mo (approximately 1 g/kg body wt/day). Serum homocysteine (HCys), malondialdehyde (MDA), diene conjugate (DC), and cholesterol levels were found to be increased, but protein carbonyl (PC) and triglyceride levels remained unchanged in the HM group as compared to controls. Cholesterol, endogenous DC, and copper-induced MDA levels were significantly higher in the...
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Yalçinkaya-Demirsöz S, Depboylu B, Dogru-Abbasoglu S, Unlüçerçi Y, Uysal M Tags: Ann Clin Lab Sci Source Type: journals

Therapy-related myelodysplastic syndrome/acute myeloid leukemia after treatment with temozolomide in a patient with glioblastoma multiforme.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Therapy-related myelodysplastic syndrome and acute leukemia after treatment with temozolomide have rarely been described in the literature. Only 10 cases in association with temozolomide have been documented. The cases included anaplastic astrocytoma (4 cases), anaplastic oligodendroglioma (2 cases), low grade astrocytoma (2 cases), low grade oligodendroglioma (1 case), and one case of secondary Philadelphia-positive acute lymphoblastic leukemia in a patient with glioblastoma multiforme. Here we report a novel case of therapy-related myelodysplastic syndrome/acute myeloid leukemia associated with der(1;7)(q10;p10) in a...
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Kim SJ, Park TS, Lee ST, Song J, Suh B, Kim SH, Jang SJ, Lee CH, Choi JR Tags: Ann Clin Lab Sci Source Type: journals

Two novel HADHB gene mutations in a Korean patient with mitochondrial trifunctional protein deficiency.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mitochondrial trifunctional protein (MTP) is a heterocomplex composed of 4 alpha-subunits containing LCEH (long-chain 2,3-enoyl-CoA hydratase) and LCHAD (long-chain 3-hydroxyacyl CoA dehydrogenase) activity, and 4 beta-subunits that harbor LCKT (long-chain 3-ketoacyl-CoA thiolase) activity. MTP deficiency is an autosomal recessive disorder that causes a clinical spectrum of diseases ranging from severe infantile cardiomyopathy to mild chronic progressive polyneuropathy. Here, we report the case of a Korean male newborn who presented with severe lactic acidosis, seizures, and heart failure. A newborn screening test and ...
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Park HD, Kim SR, Ki CS, Lee SY, Chang YS, Jin DK, Park WS Tags: Ann Clin Lab Sci Source Type: journals

Haemophilus influenzae serotype a meningitis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This work describes a case of Haemophilus influenzae serotype a meningitis in Brazil, after almost a decade since the introduction of Haemophilus influenzae serotype b conjugate vaccine. Uncertainty about the replacement of H. influenzae serotypes as a cause of invasive diseases justifies continuous surveillance, coupled with investigations of carriage rates and requirements of chemoprophylaxis in contact persons. PMID: 19880770 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: de Pádua RA, de Lima Scodro RB, Ghiraldi LD, Siqueira VL, Yamashita YK, Helbel C, Cardoso RF Tags: Ann Clin Lab Sci Source Type: journals

PCR detection of Histoplasma capsulatum var. capsulatum in whole blood of a renal transplant patient with disseminated histoplasmosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report the identification of Histoplasma capsulatum var. capsulatum from whole blood in a renal transplant patient with disseminated histoplasmosis using colorimetric microtiter-plate PCR. This modality demonstrated utility in reaching a definitive diagnosis in a timely manner. Blood fungal cultures in this case remained negative, suggesting that molecular assays may facilitate the laboratory diagnosis of disseminated histoplasmosis. PMID: 19880771 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Qualtieri J, Stratton CW, Head DR, Tang YW Tags: Ann Clin Lab Sci Source Type: journals

Lobular carcinoma in situ.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19880772 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 3, 2009 Category: Laboratory Medicine Authors: Hajdu SI, Tang P Tags: Ann Clin Lab Sci Source Type: journals

Chemical pathology of homocysteine. IV. Excitotoxicity, oxidative stress, endothelial dysfunction, and inflammation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This review considers recent advances in the chemical pathology of homocysteine in atherogenesis, oxidative metabolism, and carcinogenesis. Homocysteine is a potent excitatory neurotransmitter that binds to the N-methyl-D-aspartate (NMDA) receptor and leads to oxidative stress, cytoplasmic calcium influx, cellular apoptosis, and endothelial dysfunction. According to the adsorption-induction theory, cytoplasmic calcium influx leads to depletion of cellular adenosine triphosphate (ATP) by reaction with cytoplasmic phosphate, leading to calcium apatite deposition. Oxidative stress is caused by failure of ATP synthesis and...
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: McCully KS Tags: Ann Clin Lab Sci Source Type: journals

A New DiaSys colorimetric assay for plasma homocysteine: application in diabetic patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was conducted to evaluate the analytical performance of a new colorimetric assay for plasma homocysteine (Hcy) and to determine the influence of the method used on the frequency of hyperhomocysteinemia and on the association between Hcy and biological parameters in diabetic patients. Plasma Hcy was measured in 160 diabetic patients by high performance liquid chromatography (HPLC) in parallel with an automated colorimetric assay (DiaSys Diagnostic Systems GmbH) using the Olympus AU640 and Abbott C8000 analyzers. Plasma glucose, creatinine, folates, vitamin B(12), high-sensitivity C-reactive protein (hs-CRP), and ...
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Badiou S, Bariolet S, Dupuy AM, Sultan A, Avignon A, Cristol JP Tags: Ann Clin Lab Sci Source Type: journals

Heritability of erythrocyte sodium permeability: a possible genetic marker for hypertension.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Individuals genetically susceptible to hypertension may have preexisting membrane defects influencing cell sodium permeability. Fourteen Minnesotan families of Northern European descent were selected as having one or both progenitors with either high (HP) or low (LP) erythrocyte sodium permeability. We earlier found that over one-half of the (22)Na(+) influx into HP erythrocytes can be inhibited by micromolar amounts of furosemide, which has no apparent effect on LP erythrocytes. In these families, we find a significant midpoint parent/offspring correlation in the furosemide-sensitive component of erythrocyte (22)Na(+)...
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Lee JY, Prineas RJ, Eaton JW Tags: Ann Clin Lab Sci Source Type: journals

Truncation of histone H2A's C-terminal tail, as is typical for Ni(II)-assisted specific peptide bond hydrolysis, has gene expression altering effects.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nickel(II), capable of transforming cells and causing tumors in humans and animals, has been previously shown by us to mediate hydrolytic truncation of histone H2A's C-terminal tail by 8 amino acids in both cell-free and cell culture systems. Since H2A's C-tail is involved in maintaining chromatin structure, such truncation might alter this structure and affect gene expression. To test the latter possibility, we transfected cultured T-REx 293 human embryonic kidney cells with plasmids expressing either wild type (wt) or truncated (q) histone H2A proteins, which were either untagged or N-terminally tagged with fluoresce...
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Karaczyn AA, Cheng RY, Buzard GS, Hartley J, Esposito D, Kasprzak KS Tags: Ann Clin Lab Sci Source Type: journals

Restoration of DLC1 gene inhibits proliferation and migration of human colon cancer HT29 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
DLC1 (deleted in liver cancer-1) is a new candidate tumor suppressor gene, which is inactive in various types of human cancers including colon cancer. To study the function of DLC1, we constructed a pcDNA3.1 vector containing the DLC1 gene and transfected it into HT29 colon cancer cells that were deficient in DLC1 expression. The restoration of DLC1 expression in HT29 cells significantly inhibited cell proliferation and migration. Flow cytometry showed that DLC1 transfection into HT29 cells induced apoptosis and that the cell cycle was arrested at S-phase. Additionally, cyclinD1 mRNA and protein expression were down-re...
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Wu PP, Jin YL, Shang YF, Jin Z, Wu P, Huang PL Tags: Ann Clin Lab Sci Source Type: journals

Atypia on breast core needle biopsies: reproducibility and significance.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study analyzes the interobserver variability in interpreting atypia on breast core needle biopsies and in each category of atypia calculates the upgrade risk of carcinoma in the subsequent surgical excision. We identified 51 cases of atypia on breast core needle biopsies performed at our institution from January 2003 to August 2006. The atypia was classified into 4 categories: atypical ductal hyperplasia (ADH), atypical lobular hyperplasia (ALH), flat epithelial atypia (FEA), and atypia of undetermined significance (AUS). After a tutorial session, these cases were independently reviewed by four pathologists, whose ove...
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Darvishian F, Singh B, Simsir A, Ye W, Cangiarella JF Tags: Ann Clin Lab Sci Source Type: journals

Adrenocortical neoplasms in young children: age as a prognostic factor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report 5 cases of adrenocortical neoplasms in young children and review their clinical presentations, pathology, and follow-up data. Pathologic evaluations included histology and immunostains with p53 and Ki-67. The patients were 3 girls and 2 boys, 13-28 mo of age. The patients all presented with virilization and other hormone-related symptoms for an average duration of 6 mo. Serum testosterone levels were elevated in 3 cases. Imaging studies revealed neoplasms in the left adrenal gland in 3 cases and the right adrenal gland in 2 cases. No evidence of disease was identified at any other site of the body. The tumors wer...
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Ahmed AA Tags: Ann Clin Lab Sci Source Type: journals

Flow cytometric detection of platelet-associated immunoglobulin in patients with immune thrombocytopenic purpura and nonimmune thrombocytopenia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study demonstrates that flow cytometric detection of PAIg combined with ROC curve analysis is a convenient, sensitive, and specific test, compared to previous methods, and it is useful for the differential diagnosis of thrombocytopenic patients. PMID: 19667413 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Huh HJ, Park CJ, Kim SW, Han SH, Jang S, Chi HS Tags: Ann Clin Lab Sci Source Type: journals

Prevalence and risk factors for aspirin and clopidogrel resistance in patients with coronary artery disease or ischemic cerebrovascular disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this study was to identify possible risk factors associated with a lack of response to aspirin and clopidogrel treatments in patients with coronary or cerebral ischemic artery disease. A point-of-care analyzer, VerifyNow (Accumetrics, San Diego, CA), was used to measure adenosine-5-diphosphate and platelet P2YI2 receptor blockage to investigate the responses of a group of 197 patients to aspirin and/ or clopidogrel therapies (aspirin therapy, 178; clopidogrel therapy, 139; both drugs, 144). Of these 197 patients, 135 (68.5%) had coronary artery disease and 72 (31.5%) had ischemic cerebrovascular disease. A...
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Kim H, Lee HK, Han K, Jeon HK Tags: Ann Clin Lab Sci Source Type: journals

Molecular genetic analysis of a primitive neuroectodermal tumor arising after intracranial radiation and chemotherapy for leukemia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a case of a primitive neuroectodermal tumor (PNET) arising in the cerebellum and pons 7 yr after intracranial radiation and chemotherapy for leukemia involving the CNS. This case suggests a possible link between radiation, chemotherapy, and the formation of these tumors, with a potential new pathogenetic role for somatic inactivation of the protooncogene RET. PMID: 19667415 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Johnson MD, Moots PL, Zhuang Z, Weil RJ Tags: Ann Clin Lab Sci Source Type: journals

A novel frameshift mutation in the EYA1 gene in a Korean family with Branchio-oto-renal syndrome.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we found a Korean family showing clinical features of the disease. Mutation analysis of the EYA1 gene revealed a novel one-base-pair deletion resulting in truncated protein (c.321delT; p.Ala107fs). This is the first report of BOR syndrome caused by deletion mutation of the EYA1 gene in Korea. PMID: 19667416 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Lee JD, Kim SC, Koh YW, Lee HJ, Choi SY, Kim UK Tags: Ann Clin Lab Sci Source Type: journals

Chronic myelomonocytic leukemia with der(9)t(1;9)(q11;q34) as a sole abnormality.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The chromosomal abnormality der(9)t(1;9)(q11;q34) is a rare occurrence in patients with hematologic malignancies. As far as we know, only 3 cases of acute myeloid leukemia, 1 case of polycythemia vera, and 1 case of multiple myeloma with this derivative chromosome have been reported in the literature. Here we report the first case of der(9)t(1;9)(q11;q34) in a patient with chronic myelomonocytic leukemia (CMML). A 45-yr-old man was brought to our hospital for evaluation of pancytopenia and monocytosis. The patient's persistent monocytosis in peripheral blood and his bone marrow findings were consistent with the diagnos...
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Suh B, Park TS, Kim JS, Song J, Kim J, Yoo JH, Choi JR Tags: Ann Clin Lab Sci Source Type: journals

Surrogate Gaussian first derivative curves for determination of decision levels and confidence intervals by binary logistic regression.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It has been demonstrated that decision levels (DL) and their confidence intervals (CI) can be estimated from the second derivative, f '' (P), of the logistic regression probability curve (LRPC). Although this method generally provides smooth curves from which DL and CI can be obtained, there are datasets that generate "noisy" curves making these measurements difficult. The purpose of this study was to develop a procedure to obviate this noise, thus allowing the more facile estimation of DL and CI. Data from two clinical studies were examined. Logistic regression analysis was performed and the first derivatives, f ' (P)...
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: DeBari VA Tags: Ann Clin Lab Sci Source Type: journals

Pioneer medical scientists who died young.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19667419 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - August 12, 2009 Category: Laboratory Medicine Authors: Hajdu SI Tags: Ann Clin Lab Sci Source Type: journals

Increased esophageal regulatory T cells and eosinophil characteristics in children with eosinophilic esophagitis and gastroesophageal reflux disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Eosinophilic esophagitis (EE) and gastroesophageal reflux disease (GERD) have similar clinical presentations. The immunoregulatory mechanisms involved in both diseases are not clearly defined. We studied cellular inflammation in pediatric patients with EE and GERD compared to normal controls (NC). Pathology records were reviewed of 10 EE, 8 GERD, and 10 NC children who were seen at Texas Children's Hospital in the past 3 yr. FOXP3, CD4, CD8, CD25, eotaxin-3, and IL-5 immunohistochemical stains were performed on formalin-fixed, paraffin-embedded esophageal tissue sections and assessed by a blinded observer. The numbers ...
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Tantibhaedhyangkul U, Tatevian N, Gilger MA, Major AM, Davis CM Tags: Ann Clin Lab Sci Source Type: journals

Potential utility of CD5 immunohistochemical staining in the diagnosis of muscle tumors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, this pilot study suggests that CD5 staining may be used to differentiate benign muscle tissue from malignancy. Albeit our case series is limited, this study indicates potential utility of CD5 staining in diagnosis of muscle tumors. PMID: 19429795 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: McNab PM, DeMers NM, Schlauder S, Morgan M, Nasir A, Coppola D, Bui MM Tags: Ann Clin Lab Sci Source Type: journals

Neutrophils with toxic granulation show high fluorescence with bis(Zn2+-dipicolylamine) complex.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, measurement of FAD fluorescence intensity in neutrophils by flow cytometry after incubation with bis(Zn(2+)-dipicolylamine) complex is an easy, objective, and reliable method of detecting neutrophils with toxic granulation. PMID: 19429796 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Kim YS, Park HH, Rhee HW, Hong JI, Han K Tags: Ann Clin Lab Sci Source Type: journals

Apolipoprotein E gene polymorphism and gender.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Many studies have shown that the prevalence and onset of coronary heart disease (CHD) is sex-dependent. CHD prevalence is lower in women than in men at all ages. Furthermore, women's age of CHD onset seems to be 10 yr later. This is widely attributed to the fact that men have less favorable CHD risk factors (eg, plasma lipid profile) compared to women. Mean levels of protective high density lipoprotein cholesterol are lower, while triglyceride levels are higher in men than in women. It is possible that many of the genes involved in lipid metabolism, such as Apolipoprotein (Apo) E, as well as their polymorphisms, may be...
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Kolovou G, Damaskos D, Anagnostopoulou K, Cokkinos DV Tags: Ann Clin Lab Sci Source Type: journals

Immunotargeting of apolipoprotein E in amyloid: an initial trial in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study utilized a radiolabeled monoclonal antibody specific to human apolipoprotein E fragments and human apolipoprotein E-knock-in mice, in which AA amyloidosis was induced. Accumulation of the injected radiolabeled antibody was significantly higher in the organs of amyloidotic mice than in those of non-amyloidotic mice. Plasma clearance of the antibody did not differ between the amyloidotic and non-amyloidotic mice. The antibody was given to mice during amyloid induction but failed to prevent amyloidogenesis. The results of this initial study are encouraging, but considerable improvement is necessary, particularly in...
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Yamada T, Someya T, Fujita S Tags: Ann Clin Lab Sci Source Type: journals

Rates of vaso-vagal reactions among first time teenaged whole blood, double red cell, and plateletpheresis donors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Given the paucity of published data regarding reaction rates in younger teenaged donors, we evaluated the reaction rates in all of our first time teenaged donors after New York Blood Center lowered the minimum permissible age for blood donations from 17 to 16 yr in 2005. The overall rates of vaso-vagal reactions in donors aged 16 to 19, and those resulting in syncope, occurring in 72,769 consecutive first time whole blood, 3,822 double red cell, and 777 platelet apheresis donations were calculated. They were correlated with age and compared to those found in donors aged 20-29. Separate rates were calculated by gender, ...
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Reiss RF, Harkin R, Lessig M, Mascari J Tags: Ann Clin Lab Sci Source Type: journals

Analysis of turnaround time by subdividing three phases for outpatient chemistry specimens.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The Department of Laboratory Medicine at Asan Medical Center provides a special one-stop service to allow both doctors and outpatients to receive routine outpatient test results in a timely manner within 1 hr. We developed a new Laboratory Information System (LIS)-based monitoring system that records the laboratory turnaround time (TAT) in 3 phases and analyzes the time to complete each phase with relevant specimens. TAT is subdivided into preanalytical, analytical, and postanalytical phases based on the 4 time points when data are entered automatically into the LIS. The average TAT for 13,594 outpatient routine chemis...
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Chung HJ, Lee W, Chun S, Park HI, Min WK Tags: Ann Clin Lab Sci Source Type: journals

Changes in total CO2 measurement according to reagent cassette rotation in chemistry autoanalyzers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The quality control (QC) failure rate in the serum total carbon dioxide (TCO(2)) test increases at a higher rate than in other tests over time after calibration. The causes of the increased QC failure rate in the TCO(2) test were examined. Using a TBA200RF analyzer (Toshiba Medical Systems), the TCO(2) of the QC material was measured at 2-hr intervals and was found to decrease by up to 16.5% at 10 hr after calibration. In contrast, using the P-module and D-module analyzers (Roche Diagnostics), the TCO(2) of the QC material did not change significantly during 10 hr after calibration. When the TCO(2) level of the QC mate...
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Chung HJ, Lee W, Chun S, Kang SY, Lee WI, Park HI, Min WK Tags: Ann Clin Lab Sci Source Type: journals

Validation of the TaqMan Influenza A Detection Kit and a rapid automated total nucleic acid extraction method to detect influenza A virus in nasopharyngeal specimens.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study describes the validation of the TaqMan Influenza A Detection Kit v2.0 combined with an automated nucleic acid extraction method. The limit of detection of this assay was determined by probit regression (95% confidence interval) to be 2 influenza A/PR/8/34 (H1N1) virus particles per microlitre. One hundred and eleven specimens previously tested using the Seeplex RV assay and viral culture methods were tested using the TaqMan Influenza A Detection Kit. Compared to the aggregate gold-standard, the sensitivity and specificity of the TaqMan Influenza A Detection Kit were 100% (35/35) and 97% (74/76), respectively. Be...
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Bolotin S, De Lima C, Choi KW, Lombos E, Burton L, Mazzulli T, Drews SJ Tags: Ann Clin Lab Sci Source Type: journals

Morphoproteomic confirmation of constitutively activated mTOR, ERK, and NF-kappaB pathways in Ewing family of tumors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study examines the cytogenetic and molecular correlates and provides insight into therapeutic strategies relevant to this morphoproteomic profile. Based on a literature review, these observations appear to be the first morphoproteomic study of such pathways of convergence in tumors from EFT patients. PMID: 19429803 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Zenali MJ, Zhang PL, Bendel AE, Brown RE Tags: Ann Clin Lab Sci Source Type: journals

Autoimmune pancreatitis: case series and review of the literature.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Autoimmune pancreatitis (AuP) is a chronic pancreatic inflammation secondary to an underlying autoimmune mechanism. After early reports of a particular type of pancreatitis associated with hypergammaglobulinemia, others asserted that there is an autoimmune mechanism involved in some patients with chronic pancreatitis. In 1995 AuP was first described as a distinct clinical entity. Since then, there have been many documented cases of AuP in Japan, and now, perhaps due to increased awareness, more cases are being reported in Europe and the United States. Herein we present our experience with 3 cases of AuP and we review t...
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Shakov R, DePasquale JR, Elfarra H, Spira RS Tags: Ann Clin Lab Sci Source Type: journals

Concomitant isochromosome 17q and trisomy 14 in a patient with myelodysplastic syndrome in leukemic transformation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a novel case of simultaneous karyotypic abnormalities of isochromosome 17q and trisomy 14 in a patient with myelodysplastic syndrome (MDS) in leukemic transformation. A 66-yr-old Korean man was admitted to Severance Hospital for evaluation of pancytopenia. On the basis of bone marrow studies at 3 different stages, he was diagnosed with MDS in leukemic transformation. Chromosome karyotyping repeatedly showed the same main clonal abnormalities, including isochromosome 17q and trisomy 14. Isochromosome 17q and trisomy 14 have each been reported as rare, nonrandom recurrent chromosomal abnormalities in patients wit...
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Park TS, Song J, Lee JH, Kim JS, Yang WI, Choi JR Tags: Ann Clin Lab Sci Source Type: journals

A novel PHEX mutation in a Korean patient with sporadic hypophosphatemic rickets.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mutations including nonsense mutations, missense mutations, splicing-site mutations, insertions, and deletions in phosphate regulating genes on the X-chromosome (PHEX) are known to be responsible for X-linked hypophosphatemic rickets. The PHEX gene encodes an endopeptidase that is involved in phosphate regulation. Herein we present a female patient with sporadic hypophosphatemic rickets harboring a novel deletion mutation (c.1586_1586+1delAG; p.Glu529GlyfsX41) at exon 14 and intron 14 junction, which caused a premature termination at codon 569 and possibly produced a truncated PHEX protein. The laboratory and radiologi...
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Kim J, Yang KH, Nam JS, Choi JR, Song J, Chang M, Lee KA Tags: Ann Clin Lab Sci Source Type: journals

A novel ATP7A gross deletion mutation in a Korean patient with Menkes disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Menkes disease (MD, MIM 309400) is a fatal X-linked recessive disorder that is caused by mutations in the gene encoding ATP7A, a copper-transporting, P-type ATPase. Patients with MD are characterized by progressive hypotonia, seizures, failure to thrive, and death in early childhood. Two Korean patients were diagnosed with Menkes disease by clinical and biochemical findings. We found one missense mutation and one gross deletion in the ATP7A gene in the patients. The missense mutation in Patient 1, c.3943G>A (p.G1315R) in exon 20, was identified in a previous report. Patient 2 had a gross deletion of c.1544-?_2916+?,...
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Park HD, Moon HK, Lee J, Lee M, Lee SY, Kim JW, Ki CS Tags: Ann Clin Lab Sci Source Type: journals

Anti-tumor necrosis factor-alpha therapy provokes latent leishmaniasis in a patient with rheumatoid arthritis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It has been reported that anti-tumor necrosis factor-alpha therapy increases the risk of opportunistic infections including rare case reports of leishmaniasis. Here we report a case of latent cutaneous leishmaniasis, which was provoked by anti-tumor necrosis factor-alpha therapy in a patient with rheumatoid arthritis. PMID: 19429808 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Franklin G, Greenspan J, Chen S Tags: Ann Clin Lab Sci Source Type: journals

Nobel laureate pathologists.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19429809 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 27, 2009 Category: Laboratory Medicine Authors: Hajdu SI Tags: Ann Clin Lab Sci Source Type: journals

Review and Hypothesis: Vulnerable plaque formation from obstruction of Vasa vasorum by homocysteinylated and oxidized lipoprotein aggregates complexed with microbial remnants and LDL autoantibodies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Little attention has been paid to the function of lipoproteins as part of a nonspecific immune defense system that binds and inactivates microbes and their toxins effectively by complex formation. Because of high extra-capillary tissue pressure, aggregates of such complexes may be trapped in vasa vasorum of the major arteries. This complex formation and aggregation may be enhanced by hyperhomocysteinemia, because homocysteine thiolactone reacts with the free amino groups of apo-B to form homocysteinylated low-density lipoprotein (LDL), which is subject to spontaneous precipitation in vitro. Obstruction of the circulati...
Source: Annals of Clinical and Laboratory Science - February 11, 2009 Category: Laboratory Medicine Authors: Ravnskov U, McCully KS Tags: Ann Clin Lab Sci Source Type: journals

Different panels of markers should be used to predict mammary Paget's disease associated with in situ or invasive ductal carcinoma of the breast.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mammary Paget's disease (MPD) is a rare manifestation of breast carcinoma involving the nipple. Our objective was to identify molecular markers and molecular subtypes that may predict patients at high risk of developing MPD. Immunohistochemical (IHC) analyses were performed with antibodies to estrogen receptor (ER), progesterone receptor (PR), androgen receptor (AR), HER2, epidermal growth factor receptor (EGFR), and several cytokeratins (CK5/6, CK14, CK17, CK8, CK18) on representative sections of 121 cases of ductal carcinoma of the breast, including 28 cases with MPD, 81 cases with neither MPD nor nipple involvement,...
Source: Annals of Clinical and Laboratory Science - February 11, 2009 Category: Laboratory Medicine Authors: Lester T, Wang J, Bourne P, Yang Q, Fu L, Tang P Tags: Ann Clin Lab Sci Source Type: journals

A newly proposed semi-automated method of grading invasive lobular carcinoma: a unifying concept and correlation with prognostic markers and patient survival.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Invasive ductal carcinoma (IDC) of the breast is currently graded according to the Nottingham modification of the Scarff-Bloom-Richardson system (SBR). This system involves subjective evaluation of 3 morphologic features: tubule formation, nuclear pleomorphism, and mitosis. Our recently proposed semi-automated Nuclear and Proliferation Index [N+P] grading system for IDC has demonstrated agreement among grades and prognostic markers with better prediction of patient survival than the SBR system. Our present objective is to expand the utilization of the N+P system to grading invasive lobular carcinoma (ILC). Fifty-eight ...
Source: Annals of Clinical and Laboratory Science - February 11, 2009 Category: Laboratory Medicine Authors: Stevens E, Kimler BF, Davis MK, Fan F, Thomas P, Wang XY, Damjanov I, Tawfik OW Tags: Ann Clin Lab Sci Source Type: journals

Rapid quantitation of hemoglobin S in sickle cell patients using the Variant II Turbo analyzer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A rapid ( approximately 90 sec), fully automated method is described for quantifying hemoglobin S (HbS) by high performance liquid chromatography (HPLC) using the Bio-Rad Variant II Turbo analyzer. Although this instrument is designed to quantify only blood hemoglobin A1c (HbA1c), we show that it can also quantify accurately, without modification, HbS levels in sickle cell patients, provided the blood samples meet certain conditions. The samples should contain detectable hemoglobin F (HbF), but should not contain hemoglobin C (HbC). Under these conditions, blood HbS levels obtained by this method correlate well with th...
Source: Annals of Clinical and Laboratory Science - February 11, 2009 Category: Laboratory Medicine Authors: Edwards HD, Sabio H, Shihabi ZK Tags: Ann Clin Lab Sci Source Type: journals

Polymorphic analysis of the Lutheran blood group system in Chinese.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we performed genetic polymorphism analyses by molecular techniques of the Lutheran blood group system in Chinese subjects. Blood samples were collected from randomly-selected healthy donors and analyzed by PCR-RFLP or SBT methods. LU1/2(Lu(a)/Lu(b)), LU6/9, LU8/14, and LU18/LU19(Au(a)/Au(b)) antigen polymorphisms were detected as follows: 1102 individuals were diagnosed as Lu(a-b+) type; 117 individuals were all LU(6+9-) genotypes; 119 individuals were all LU(8+14-) genotypes. Among 368 individuals, 278 showed homozygous nt1615A, 6 showed homozygous nt1615G, and 84 showed nt1615A/G heterozygosity. The gene f...
Source: Annals of Clinical and Laboratory Science - February 11, 2009 Category: Laboratory Medicine Authors: Zeng JQ, Deng ZH, Yang BC, Liang YL, Su YQ, Li da C, Yu Q Tags: Ann Clin Lab Sci Source Type: journals

IgE anti-varicella zoster virus and other immune responses before, during, and after shingles.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Blood lymphocyte distributions, serum immunoglobulin and cytokine levels, and serum IgE and IgG anti-varicella zoster virus (VZV) levels were measured in an atopic girl (age 15 yr) who developed shingles 10 yr after infection with chicken pox. Before, during, and 5 months after the shingles episode, the child's distributions of blood lymphocytes (excluding CD23+) and serum immunoglobulin levels (excluding IgE) were within the normal ranges. Her blood level of CD23+ lymphocytes decreased >50% during the shingles episode and remained low thereafter. Her serum level of IgE was elevated before and during shingles (154 a...
Source: Annals of Clinical and Laboratory Science - February 11, 2009 Category: Laboratory Medicine Authors: Smith-Norowitz TA, Josekutty J, Lev-Tov H, Kohlhoff S, Norowitz KB, Silverberg JI, Chice S, Durkin HG, Bluth MH Tags: Ann Clin Lab Sci Source Type: journals