The Syntheses of Chromium Aluminum Carbide (Cr < sub > 2 < /sub > AlC) MAX  Phase and Cr < sub > 2 < /sub > CTx MXene and Investigation of Their Antimicrobial Properties
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04910-w. Online ahead of print.ABSTRACTChromium aluminum carbide (Cr2AlC) MAX phase and Cr2CTx (MXene-Cr) were synthesized by the pressureless sintering method and hydrothermal method, respectively. In addition to this, the free radical scavenging activities (FRSA) of MAX-Cr phase and MXene-Cr compounds were tested and compared with ascorbic acid and trolox as standard compounds. The obtained FRSA results of MAX-Cr phase and MXene-Cr were 42.82 and 59.64%, respectively, at 100 mg/L concentration. MXene-Cr showed a 66.90% inhibitory effect on α-amylase at 200 mg...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Gul Kaya Eda Ondul Koc Sadin Özdemir M Serkan Yal çın Kasim Ocakoglu Nadir Dizge Source Type: research

Exploring the Mechanism of Acupuncture's Influence on the Protease Activity and Free Radical Damage in Synovial Fluid of Rheumatoid Arthritis Rats Induced by Type II Collagen from the Release of Active Oxygen
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04932-4. Online ahead of print.ABSTRACTAcupuncture was studied to investigate the mechanism of its effect on protease vitality and free radical damage in Type I CIA rats induced by type II collagen. The study divided rats into a control group (injected with physiological saline, n = 10), a model group (injected with type II collagen, n = 10), and an intervention group (injected with type II collagen + acupuncture ST36 and GB39, 3 times a week, for a total of 4 weeks, n = 10) based on the different injected drugs. Then, various indicators of the mice were experim...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Kaiying Cui Dezhong Bi Fuyan Wang Pengfei Hou Jinxing Guo Zhanwang Xu Source Type: research

Improving the Catalytic Properties of Xylanase from Alteromones Macleadii H35 Through Sequence Analysis
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04936-0. Online ahead of print.ABSTRACTEndo-1,4-β-xylanase is a key xylanolytic enzyme, and our study aimed to enhance the catalytic properties of Alteromones Macleadii xylanase (Xyn ZT-2) through sequence-guided design approach. Analysis of the amino acid sequence revealed highly conserved residues near the active site, with few differences. Introducing various mutations allowed us to modify the enzyme's catalytic performance. Particularly, the A152G mutation led to a 9.8-fold increase in activity and a 23.2-fold increase in catalytic efficiency. Moreover, A15...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Caixia Cui Jia Xu Juntao Wu Ningning Wang Zhao Zhang Chenyan Zhou Source Type: research

Correlation of Differentially Expressed lncRNAs with Intestinal Flora Imbalance, Small Intestinal Permeability, and Glucose Uptake in T2DM Mice
This study aimed to investigate the correlation between differentially expressed lncRNAs in mice with type 2 diabetes mellitus (T2DM) and alterations in the intestinal flora and intestinal pathology. A T2DM mouse model was constructed by feeding mice a high-fat diet. Serum fat metabolism-related indices and insulin levels were biochemically detected. Serum inflammatory factors (IL-1β, IL-6, TNF-α, IL-10) and endotoxin (LPS) were measured by ELISA. Histopathological changes in the small intestines of mice were observed by HE. The short-chain fatty acid (SCFA) content was analyzed using GC-MS. Analysis of altered intestina...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Shufang Xu Heng Zhang Hui Zou Zhitao Chen Ting Jiang Mengjun Huang Source Type: research

The Impact of Transcriptional Profiling Cadherin Family and Therapeutic Approaches of Gastric Cancer: A Translational Outlook on Multi-omics Data Analysis
This study used multi-omics data to examine the cadherin genes that were differentially regulated in gastric cancer. Differential expression of genes, epigenetic, molecular alterations, and protein expression analyses was conducted. Male SD rats were given N-methyl-N-nitrosourea (MNU) to induce stomach carcinoma in order to verify the activation of cadherin genes. CDH5, CDH6, CDH11, and CDH24 levels were found to be considerably higher in gastric cancer and may serve as useful indicators of stomach adenocarcinoma (STAD). Cadherin genes with variable expression had considerably more promoter methylation in cancers than in n...
Source: Applied Biochemistry and Biotechnology - March 26, 2024 Category: Biochemistry Authors: Huan Wang Baomin Zhang Source Type: research

Valorization of Chicken Viscera as Natural Raw Material Source: Application to Hydrolysis of Fatty Esters for Sewage Treatment
Appl Biochem Biotechnol. 2024 Mar 26. doi: 10.1007/s12010-024-04915-5. Online ahead of print.ABSTRACT"Chicken viscera" constitutes very abundant domestic wastes interestingly investigated in the present paper. The efficiency of this crude slaughter co-product of high protein component, as biocatalyst, for the hydrolysis of fatty acid esters was reported and that, without any pre-treatment. The crude chicken intestine powder (CIP) has shown a high reactivity for the hydrolysis of fatty esters. Two biocatalyst preparations were independently explored for the bioresolution of sec-phenyl alkyl carbinol esters: the CIP preparat...
Source: Applied Biochemistry and Biotechnology - March 26, 2024 Category: Biochemistry Authors: Nedjma Melais Louisa Aribi-Zouioueche Olivier Riant Source Type: research

The Functional Study of Response Regulator ArlR Mutants in Staphylococcus Aureus
This study aims to investigate the role of the arlR deletion mutant in the detection and activation of S. aureus. We created an arlR deleted mutant and complementary strains and characterized their impact on the strains using partial growth measurement. The quantitative real-time PCR was performed to determine the expression of icaA, and the microscopic images of adherent cells were captured at the optical density of 600 to determine the primary bacterial adhesion. The biofilm formation assay was utilized to investigate the number of adherent cells using crystal violet staining. Eventually, the Triton X-100 autolysis assay...
Source: Applied Biochemistry and Biotechnology - March 26, 2024 Category: Biochemistry Authors: Jinhong Zhou Moath Refat Yucheng Guo Jiaxin Zhang Min Jiao Wenbo He Xiaoyu He Mai A Rabie Zhenlin Ouyang Fang Zheng Source Type: research

Ginsenoside Rb1 Deters Cell Proliferation, Induces Apoptosis, Alleviates Oxidative Stress, and Antimetastasis in Oral Squamous Carcinoma Cells
Appl Biochem Biotechnol. 2024 Mar 26. doi: 10.1007/s12010-024-04880-z. Online ahead of print.ABSTRACTThere are numerous therapeutic applications for ginsenoside Rb1 (GRb1), the primary saponin derived from ginseng root. According to earlier research, ginsenoside Rb1 causes apoptosis and reduces the cell cycle. Its adverse effects, especially those on the development of the embryo, still need to be thoroughly studied. A host's lifestyle choices, including smoking, drinking too much alcohol, using tobacco products, and having an HPV infection, can increase the risk of oral squamous cell carcinoma (OSCC), one of the most prev...
Source: Applied Biochemistry and Biotechnology - March 26, 2024 Category: Biochemistry Authors: Le An Yang Yu Long He Xu Xiao Pengcheng Li Source Type: research

The Impact of Transcriptional Profiling Cadherin Family and Therapeutic Approaches of Gastric Cancer: A Translational Outlook on Multi-omics Data Analysis
This study used multi-omics data to examine the cadherin genes that were differentially regulated in gastric cancer. Differential expression of genes, epigenetic, molecular alterations, and protein expression analyses was conducted. Male SD rats were given N-methyl-N-nitrosourea (MNU) to induce stomach carcinoma in order to verify the activation of cadherin genes. CDH5, CDH6, CDH11, and CDH24 levels were found to be considerably higher in gastric cancer and may serve as useful indicators of stomach adenocarcinoma (STAD). Cadherin genes with variable expression had considerably more promoter methylation in cancers than in n...
Source: Applied Biochemistry and Biotechnology - March 26, 2024 Category: Biochemistry Authors: Huan Wang Baomin Zhang Source Type: research

Valorization of Chicken Viscera as Natural Raw Material Source: Application to Hydrolysis of Fatty Esters for Sewage Treatment
Appl Biochem Biotechnol. 2024 Mar 26. doi: 10.1007/s12010-024-04915-5. Online ahead of print.ABSTRACT"Chicken viscera" constitutes very abundant domestic wastes interestingly investigated in the present paper. The efficiency of this crude slaughter co-product of high protein component, as biocatalyst, for the hydrolysis of fatty acid esters was reported and that, without any pre-treatment. The crude chicken intestine powder (CIP) has shown a high reactivity for the hydrolysis of fatty esters. Two biocatalyst preparations were independently explored for the bioresolution of sec-phenyl alkyl carbinol esters: the CIP preparat...
Source: Applied Biochemistry and Biotechnology - March 26, 2024 Category: Biochemistry Authors: Nedjma Melais Louisa Aribi-Zouioueche Olivier Riant Source Type: research

The Functional Study of Response Regulator ArlR Mutants in Staphylococcus Aureus
This study aims to investigate the role of the arlR deletion mutant in the detection and activation of S. aureus. We created an arlR deleted mutant and complementary strains and characterized their impact on the strains using partial growth measurement. The quantitative real-time PCR was performed to determine the expression of icaA, and the microscopic images of adherent cells were captured at the optical density of 600 to determine the primary bacterial adhesion. The biofilm formation assay was utilized to investigate the number of adherent cells using crystal violet staining. Eventually, the Triton X-100 autolysis assay...
Source: Applied Biochemistry and Biotechnology - March 26, 2024 Category: Biochemistry Authors: Jinhong Zhou Moath Refat Yucheng Guo Jiaxin Zhang Min Jiao Wenbo He Xiaoyu He Mai A Rabie Zhenlin Ouyang Fang Zheng Source Type: research

Ginsenoside Rb1 Deters Cell Proliferation, Induces Apoptosis, Alleviates Oxidative Stress, and Antimetastasis in Oral Squamous Carcinoma Cells
Appl Biochem Biotechnol. 2024 Mar 26. doi: 10.1007/s12010-024-04880-z. Online ahead of print.ABSTRACTThere are numerous therapeutic applications for ginsenoside Rb1 (GRb1), the primary saponin derived from ginseng root. According to earlier research, ginsenoside Rb1 causes apoptosis and reduces the cell cycle. Its adverse effects, especially those on the development of the embryo, still need to be thoroughly studied. A host's lifestyle choices, including smoking, drinking too much alcohol, using tobacco products, and having an HPV infection, can increase the risk of oral squamous cell carcinoma (OSCC), one of the most prev...
Source: Applied Biochemistry and Biotechnology - March 26, 2024 Category: Biochemistry Authors: Le An Yang Yu Long He Xu Xiao Pengcheng Li Source Type: research

Zoledronic Acid Accelerates ER Stress-Mediated Inflammation by Increasing PDE4B Expression in Bisphosphonate-Related Osteonecrosis of the Jaw
Appl Biochem Biotechnol. 2024 Mar 25. doi: 10.1007/s12010-024-04859-w. Online ahead of print.ABSTRACTLong-term administration of bisphosphonates can lead to a significant side effect known as bisphosphonate-related osteonecrosis of the jaw (BRONJ). Although macrophage-mediated inflammation has been established as an important factor in BRONJ, the underlying mechanism remains elusive. In the current study, the roles of endoplasmic reticulum (ER) stress in zoledronic acid (ZOL)-induced inflammation were analyzed in macrophages, and the regulatory mechanism of ER stress activation was next investigated. An in vitro model of B...
Source: Applied Biochemistry and Biotechnology - March 25, 2024 Category: Biochemistry Authors: Qu Xz Sun Zq Liu L Ong Hs Source Type: research

Neferine Pretreatment Attenuates Isoproterenol-Induced Cardiac Injury Through Modulation of Oxidative Stress, Inflammation, and Apoptosis in Rats
In conclusion, the study provides strong evidence that pretreatment with neferine protects rats from ISO-induced heart damage.PMID:38526658 | DOI:10.1007/s12010-024-04917-3 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - March 25, 2024 Category: Biochemistry Authors: Xiaoqian Sun Yongwen Gu Xinghua Liu Praveen Kumar Korla Junjun Hao Source Type: research

Mechanism of Cell-Killing Activity of Plantaricin LD1 Against Escherichia coli ATCC 25922
In this study, we have performed a detailed mechanism of action of plantaricin LD1 against Escherichia coli ATCC 25922 considering Micrococcus luteus MTCC 106 as control. The plantaricin LD1 showed a minimum inhibitory concentration (MIC) of 34.57 µg/mL and a minimum bactericidal concentration (MBC) of 138.3 µg/mL against M. luteus MTCC 106, whereas MIC 69.15 µg/mL and MBC 276.6 µg/mL were found against E. coli ATCC 25922. The efflux of potassium ions, dissipation of membrane potential (∆ψ), and transmembrane pH gradient (∆pH) of plantaricin LD1-treated cells suggested the membrane-acting nature of plantaricin LD1...
Source: Applied Biochemistry and Biotechnology - March 25, 2024 Category: Biochemistry Authors: Manoj Kumar Yadav Santosh Kumar Tiwari Source Type: research