Constraining activity and growth substrate of fungal decomposers via assimilation patterns of inorganic carbon and water into lipid biomarkers
This study developed and applied a dual stable isotope assay (13C-dissolved inorganic carbon/2H) to improve the investigation of fungal activity in the environment. By determining the incorporation patterns of hydrogen and carbon into fungal lipids, this assay delivers estimates of fungal activity and the different metabolic pathways that they employ in ecological and environmental systems.PMID:38527003 | DOI:10.1128/aem.02065-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - March 25, 2024 Category: Microbiology Authors: Stanislav Jabinski Wesley D M Rangel Marek Kop áček Veronika J ílková Jan Jansa Travis B Meador Source Type: research

Constraining activity and growth substrate of fungal decomposers via assimilation patterns of inorganic carbon and water into lipid biomarkers
This study developed and applied a dual stable isotope assay (13C-dissolved inorganic carbon/2H) to improve the investigation of fungal activity in the environment. By determining the incorporation patterns of hydrogen and carbon into fungal lipids, this assay delivers estimates of fungal activity and the different metabolic pathways that they employ in ecological and environmental systems.PMID:38527003 | DOI:10.1128/aem.02065-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - March 25, 2024 Category: Microbiology Authors: Stanislav Jabinski Wesley D M Rangel Marek Kop áček Veronika J ílková Jan Jansa Travis B Meador Source Type: research

Tartrate fermentation with H < sub > 2 < /sub > production by a new member of < em > Sporomusaceae < /em > enriched from rice paddy soil
Appl Environ Microbiol. 2024 Mar 22:e0235123. doi: 10.1128/aem.02351-23. Online ahead of print.ABSTRACTIn rice paddies, soil and plant-derived organic matter are degraded anaerobically to methane (CH4), a powerful greenhouse gas. The highest rate of methane emission occurs during the reproductive stage of the plant when mostly dicarboxylic acids are exudated by the roots. The emission of methane at this stage depends largely on the cooperative interaction between dicarboxylic acid-fermenting bacteria and methanogenic archaea in the rhizosphere. The fermentation of tartrate, one of the major acids exudated, has been scarcel...
Source: Applied and Environmental Microbiology - March 22, 2024 Category: Microbiology Authors: Luciana Pereira-Mora Leandro D Guerrero Leonardo Erijman Ana Fern ández-Scavino Source Type: research

Tartrate fermentation with H < sub > 2 < /sub > production by a new member of < em > Sporomusaceae < /em > enriched from rice paddy soil
Appl Environ Microbiol. 2024 Mar 22:e0235123. doi: 10.1128/aem.02351-23. Online ahead of print.ABSTRACTIn rice paddies, soil and plant-derived organic matter are degraded anaerobically to methane (CH4), a powerful greenhouse gas. The highest rate of methane emission occurs during the reproductive stage of the plant when mostly dicarboxylic acids are exudated by the roots. The emission of methane at this stage depends largely on the cooperative interaction between dicarboxylic acid-fermenting bacteria and methanogenic archaea in the rhizosphere. The fermentation of tartrate, one of the major acids exudated, has been scarcel...
Source: Applied and Environmental Microbiology - March 22, 2024 Category: Microbiology Authors: Luciana Pereira-Mora Leandro D Guerrero Leonardo Erijman Ana Fern ández-Scavino Source Type: research

Tartrate fermentation with H < sub > 2 < /sub > production by a new member of < em > Sporomusaceae < /em > enriched from rice paddy soil
Appl Environ Microbiol. 2024 Mar 22:e0235123. doi: 10.1128/aem.02351-23. Online ahead of print.ABSTRACTIn rice paddies, soil and plant-derived organic matter are degraded anaerobically to methane (CH4), a powerful greenhouse gas. The highest rate of methane emission occurs during the reproductive stage of the plant when mostly dicarboxylic acids are exudated by the roots. The emission of methane at this stage depends largely on the cooperative interaction between dicarboxylic acid-fermenting bacteria and methanogenic archaea in the rhizosphere. The fermentation of tartrate, one of the major acids exudated, has been scarcel...
Source: Applied and Environmental Microbiology - March 22, 2024 Category: Microbiology Authors: Luciana Pereira-Mora Leandro D Guerrero Leonardo Erijman Ana Fern ández-Scavino Source Type: research

Persistence and free chlorine disinfection of human coronaviruses and their surrogates in water
This study addresses an important knowledge gap on enveloped virus persistence and disinfection in water. Results have immediate practical applications for shaping evidence-based water policies, particularly in the development of disinfection strategies for pathogenic virus control.PMID:38511945 | DOI:10.1128/aem.00055-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - March 21, 2024 Category: Microbiology Authors: Mengyang Zhang Michelle Wei Leong William A Mitch Catherine A Blish Alexandria Boehm Source Type: research

Persistence and free chlorine disinfection of human coronaviruses and their surrogates in water
This study addresses an important knowledge gap on enveloped virus persistence and disinfection in water. Results have immediate practical applications for shaping evidence-based water policies, particularly in the development of disinfection strategies for pathogenic virus control.PMID:38511945 | DOI:10.1128/aem.00055-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - March 21, 2024 Category: Microbiology Authors: Mengyang Zhang Michelle Wei Leong William A Mitch Catherine A Blish Alexandria Boehm Source Type: research

Articles of Significant Interest in This Issue
Appl Environ Microbiol. 2024 Mar 20;90(3):e0042624. doi: 10.1128/aem.00426-24. Epub 2024 Mar 20.NO ABSTRACTPMID:38506507 | DOI:10.1128/aem.00426-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - March 20, 2024 Category: Microbiology Source Type: research

< em > Pediococcus < /em > spp. < em > - < /em > mediated competition interaction within Daqu microbiota determines the temperature formation and metabolic profiles
This study adopted high-throughput sequencing and monitored the dynamic fluctuations of metabolites and environmental factors to identify the pivotal microorganism responsible for the alterations in interaction patterns of functional keystone taxa and quality decline in the fermentation system of different operational areas during the in situ fermentation process that had been mainly attributed to operational taxonomic unit (OTU)_22 (Pediococcus acidilactici). Additionally, we used isothermal microcalorimetry, plate inhibition experiments, and in vitro simulation fermentation experiments to explore the impact of Pediococcu...
Source: Applied and Environmental Microbiology - March 20, 2024 Category: Microbiology Authors: Shenglu Wu Jun Lu Changwen Li Hai Du Yan Xu Source Type: research

Conditional expression of FumA in < em > Aspergillus niger < /em > enhances synthesis of L-malic acid
This study focuses on enhancing L-malic acid synthesis by modifying the tricarboxylic acid cycle within the mitochondria of Aspergillus niger. We emphasize the significant role of fumarase in converting fumaric acid into L-malic acid, enhancing our understanding of metabolic pathways in A. niger. The precise regulation of fumA is highlighted as a key factor in enhancing L-malic acid production. Furthermore, this research introduces a stringent conditional promoter (PmfsA), exclusively activated by CaCO3. The utilization of PmfsA for fumA expression resulted in heightened L-malic acid titers. The progress in metabolic engin...
Source: Applied and Environmental Microbiology - March 20, 2024 Category: Microbiology Authors: Chi Zhang Man Shi Yingyan Xu Dongdong Yang Ling Lu Feng Xue Qing Xu Source Type: research

Persistence of human respiratory viral RNA in wastewater-settled solids
This study investigated the decay kinetics of genomic nucleic-acids of seven human respiratory viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), respiratory syncytial virus (RSV), human coronavirus (HCoV)-OC43, HCoV-229E, HCoV-NL63, human rhinovirus (HRV), and influenza A virus (IAV), as well as pepper mild mottle virus (PMMoV) in wastewater solids. Viruses (except for PMMoV) were spiked into wastewater solids and their concentrations were followed for 50 days at three different temperatures (4°C, 22°C, and 37°C). Viral genomic RNA decayed following first-order kinetics with decay rate con...
Source: Applied and Environmental Microbiology - March 19, 2024 Category: Microbiology Authors: Mengyang Zhang Laura Roldan-Hernandez Alexandria Boehm Source Type: research

Exploring functional genes' correlation with ( < em > S < /em > )-equol concentration and new daidzein racemase identification
This study examines the association between functional genes and (S)-EQ production, highlighting a potential correlation between the DZNR gene and (S)-EQ content. Various types of DZNR may be linked to the regulation of (S)-EQ synthesis. Furthermore, the identification of a new DDRC gene offers promising prospects for enhancing in vitro (S)-EQ production.PMID:38501861 | DOI:10.1128/aem.00007-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - March 19, 2024 Category: Microbiology Authors: Yun-Fei Hu Shu Luo Sheng-Qi Wang Ke-Xin Chen Wei-Xuan Zhong Bai-Yuan Li Lin-Yan Cao Hua-Hai Chen Ye-Shi Yin Source Type: research

Protein disulfide isomerase 1 is required for RodA assembling-based conidial hydrophobicity of < em > Aspergillus fumigatus < /em >
In conclusion, Pdi1 ensures the correct folding of RodA in the inner layer of conidia, facilitating its secretion into the outer layer of the cell wall and allowing self-assembly of the hydrophobic layer. This study has identified a regulatory element for conidia rodlet assembly.IMPORTANCEAspergillus fumigatus is the major cause of invasive aspergillosis, which is mainly transmitted by the inhalation of conidia. The spread of conidia is largely dependent on their hydrophobicity, which is primarily attributed to the self-assembly of the hydrophobic protein RodA on the cell wall. However, the mechanisms underlying RodA secre...
Source: Applied and Environmental Microbiology - March 19, 2024 Category: Microbiology Authors: Xiaotao Hu Yao Zhou Renning Liu Jing Wang Lingyan Guo Xiaofei Huang Jingyi Li Yunfeng Yan Feiyun Liu Xueying Li Xinyu Tan Yiqing Luo Ping Wang Shengmin Zhou Source Type: research

Persistence of human respiratory viral RNA in wastewater-settled solids
This study investigated the decay kinetics of genomic nucleic-acids of seven human respiratory viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), respiratory syncytial virus (RSV), human coronavirus (HCoV)-OC43, HCoV-229E, HCoV-NL63, human rhinovirus (HRV), and influenza A virus (IAV), as well as pepper mild mottle virus (PMMoV) in wastewater solids. Viruses (except for PMMoV) were spiked into wastewater solids and their concentrations were followed for 50 days at three different temperatures (4°C, 22°C, and 37°C). Viral genomic RNA decayed following first-order kinetics with decay rate con...
Source: Applied and Environmental Microbiology - March 19, 2024 Category: Microbiology Authors: Mengyang Zhang Laura Roldan-Hernandez Alexandria Boehm Source Type: research

Exploring functional genes' correlation with ( < em > S < /em > )-equol concentration and new daidzein racemase identification
This study examines the association between functional genes and (S)-EQ production, highlighting a potential correlation between the DZNR gene and (S)-EQ content. Various types of DZNR may be linked to the regulation of (S)-EQ synthesis. Furthermore, the identification of a new DDRC gene offers promising prospects for enhancing in vitro (S)-EQ production.PMID:38501861 | DOI:10.1128/aem.00007-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - March 19, 2024 Category: Microbiology Authors: Yun-Fei Hu Shu Luo Sheng-Qi Wang Ke-Xin Chen Wei-Xuan Zhong Bai-Yuan Li Lin-Yan Cao Hua-Hai Chen Ye-Shi Yin Source Type: research