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The expression of transferrin binding protein in the turtle nervous system.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Transferrin binding protein (TfBP) is a cytoplasmic glycoprotein that was originally isolated from the chick oviduct. As we previously demonstrated the constitutive expression of TfBP in the avian nervous system, in this study we examined whether TfBP is expressed in the reptilian nervous system. In accordance with previous findings in the chicken, oligodendrocytes were most prominently labeled by antiserum to TfBP. Great variability was observed between different regions of the central nervous system (CNS) in terms of TfBP-labeled oligodendrocyte numbers. In the retina, TfBP was localized specifically in the cells tha...
Source: Arch Histol Cytol - March 1, 2009 Category: Cytology Authors: Park SW, Lee HN, Jeon GS, Sim KB, Cho IH, Cho SS Tags: Arch Histol Cytol Source Type: journals

Effects of basic fibroblast growth factor on the development of the stem cell properties of human dental pulp cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We isolated adherent fibroblastic cells after collagenase and dispase treatment of human dental pulp. When human dental pulp cells (hDPCs) were cultured in the presence of basic fibroblast growth factor (bFGF), the ratio of hDPCs in the S-phase was significantly higher in comparison with incubation without bFGF. The ratio of hDPCs expressing STRO-1 as a marker of stem cell populations increased approximately eightfold in the presence of bFGF as opposed to that in the absence of bFGF. We demonstrated the characterization and distinctiveness of the hDPCs and showed that, when cultured with the medium containing serum and...
Source: Arch Histol Cytol - March 1, 2009 Category: Cytology Authors: Morito A, Kida Y, Suzuki K, Inoue K, Kuroda N, Gomi K, Arai T, Sato T Tags: Arch Histol Cytol Source Type: journals

Characterization of the sugar chain expression of normal term human placental villi using lectin histochemistry combined with immunohistochemistry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The general sugar expression pattern was studied in 9 normal full-term human placentas by the use of 21 individual lectins in combination with immunohistochemistry for various markers to understand the function of the placenta as the site of feto-maternal interactions. In mature intermediate and terminal villi, the brush border of the syncytiotrophoblast layer strongly expressed GlcNAc (as stained by WGA, S-WGA, DSL lectins) but weakly expressed sialic acid (Mal II, SNA). The cytoplasm of the syncytiotrophoblast layer showed weak expressions of GlcNAc and Gal/GalNAc with granular patterns. The cytotrophoblast layer, as...
Source: Arch Histol Cytol - March 1, 2009 Category: Cytology Authors: Tatsuzuki A, Ezaki T, Makino Y, Matsuda Y, Ohta H Tags: Arch Histol Cytol Source Type: journals

Expression of the nerve growth factor-induced gene B-beta in the developing rat brain and retina.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The nerve growth factor-induced gene B-beta (NGFI-Bbeta, Nurr1) is a member of the nuclear receptor superfamily that is expressed predominantly in the central nervous system. We used an antibody against the human NGFI-Bbeta to observe the protein expression in neuronal cells in the retina, cerebral neocortex, and midbrain of humans and rats. To provide further insight into the role of NGFI-Bbeta in the differentiation of neuronal cells, we also examined the expression of NGFI-Bbeta in rat ontogeny. A few cells in the midbrain showed the expression of NGFIBbeta from 12 days of gestation, and NGFI-Bbeta positive cells in...
Source: Arch Histol Cytol - March 1, 2009 Category: Cytology Authors: Li Y, Ohashi R, Naito M Tags: Arch Histol Cytol Source Type: journals

The microstructure of lingual papillae in the Egyptian fruit bat (Rousettus aegyptiacus) as observed by light microscopy and scanning electron microscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The microstructure of lingual papillae on the dorsal surface of the tongue of adult Egyptian fruit bats was examined by light microscopy (LM) and scanning electron microscopy (SEM). This elongated tongue with a rounded apex is approximately 3 cm long -- including the 1.7cm length of the anterior free part of the tongue -- which facilitates considerable freedom of movement. The surface of the tongue has four types of lingual papillae: two types of mechanical papillae -- filiform and conical papillae, and two types of gustatory papillae -- fungiform and vallate papillae. Most numerous are filiform papillae with well deve...
Source: Arch Histol Cytol - March 1, 2009 Category: Cytology Authors: Jackowiak H, Trzcielińska-Lorych J, Godynicki S Tags: Arch Histol Cytol Source Type: journals

Expression of osteogenic proteins during the intrasplenic transplantation of Meckel's chondrocytes: A histochemical and immunohistochemical study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we transplanted cell pellets of Meckel's chondrocytes into isogenic mouse spleens and analyzed their phenotypic transformation into osteogenic cells using histological and immunohistochemical methods. With the increasing duration of transplantation, chondrocytes were incorporated into splenic tissues and formed a von Kossa-positive calcified matrix containing calcium and phosphoric acid, similar to that of intact bone. Type I, II, and X collagens, and the bone-marker proteins osteocalcin, osteopontin, osteonectin, and bone morphogenetic protein-2 (BMP-2) were immunolocalized in the matrix formed by the trans...
Source: Arch Histol Cytol - March 1, 2009 Category: Cytology Authors: Ishizeki K, Kagiya T, Fujiwara N, Otsu K, Harada H Tags: Arch Histol Cytol Source Type: journals

Accumulation of stress-related proteins within the glomeruli of the rat olfactory bulb following damage to olfactory receptor neurons.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The expression of stress-responsive proteins, such as nestin and a 27-kDa heat-shock protein (HSP27), was immunohistochemically examined in order to demonstrate glial responses in the rat olfactory bulb following sensory deprivation. At 3 days to 1 week after sensory deprivation, numerous nestin-expressing cells appeared within the glomerulus of the olfactory bulb. These cells were regarded as reactive astrocytes since they were immunoreactive for glial fibrillary acidic protein and showed hypertrophic features. The glomeruli, in which nestin-immunoreactive astrocytes were localized, were filled with degenerating termi...
Source: Arch Histol Cytol - December 1, 2008 Category: Cytology Authors: Hirata K, Kanemaru T, Minohara M, Togo A, Kira J Tags: Arch Histol Cytol Source Type: journals

Microtubule remodeling mediates the inhibition of store-operated calcium entry (SOCE) during mitosis in COS-7 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Regulation of the intracellular calcium ion concentration ([Ca(2+)](i)) is critical, because calcium signaling controls diverse and vital cellular processes such as secretion, proliferation, division, gene transcription, and apoptosis. Store-operated calcium entry (SOCE) is the main mechanism through which non-excitable cells replenish and thus maintain this delicate balance. There is limited evidence which indicates that SOCE may be inhibited during mitosis, and the mechanisms leading to the presumed inhibition has not been elucidated. In the present study, we examined and compared the [Ca(2+)](i) dynamics of COS-7 ce...
Source: Arch Histol Cytol - December 1, 2008 Category: Cytology Authors: Russa AD, Ishikita N, Masu K, Akutsu H, Saino T, Satoh Y Tags: Arch Histol Cytol Source Type: journals

Dipyridamole inhibits intracellular calcium transients in isolated rat arteriole smooth muscle cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Dipyridamole, an inhibitor of adenosine uptake as well as a cGMP phosphodiesterase inhibitor, is commonly used in prophylactic therapy for patients with angina pectoris. However, the effects of dipyridamole on systemic blood vessels, especially on the peripheral vascular system, are not well understood. Therefore, the effect of dipyridamole on ATP-induced arteriole contraction was examined with special reference to intracellular Ca(2+) concentration ([Ca(2+)](i)) using real-time confocal microscopy. In cases of 0.1-10microM range, dipyridamole induced only slight [Ca(2+)](i) decreases in smooth muscle cells of both tes...
Source: Arch Histol Cytol - December 1, 2008 Category: Cytology Authors: Saino T, Misaki T, Matsuura M, Shikanai T, Satoh Y Tags: Arch Histol Cytol Source Type: journals

Ultrastructure of tracheal epithelial cells migrating in an in vivo environment.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The tracheal epithelium can be induced to move as a cellular sheet by heterotopic transplantation, which offers the opportunity to observe migrating cells as a group in an in vivo environment. We therefor investigated the ultrastructural characteristics of migrating tracheal epithelial cells with special reference to the moving front using this transplantation. The migrating epithelial cells underwent squamous metaplasia and lost their differentiated characteristics such as cilia or secretory granules. Several unique observations were made concerning the mechanism of mobility: one is that epithelial cells in the front ...
Source: Arch Histol Cytol - December 1, 2008 Category: Cytology Authors: Sawada H, Tanaka H, Ono M Tags: Arch Histol Cytol Source Type: journals

Cellular and subcellular localizations of nonheme ferric and ferrous iron in the rat brain: a light and electron microscopic study by the perfusion-Perls and -Turnbull methods.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we focused on the cellular and subcellular localizations of nonheme ferric (Fe(III)) and ferrous (Fe(II)) iron in the adult female rat brain using light and electron microscopic histochemistry. Although Fe(II) deposition was much less dominant than Fe(III), the brain contained iron in both forms. Among the cellular elements of the brain, oligodendrocytes were numerically the most prominent and heavily iron-storing cells. Pericapillary astrocytes and sporadic microglial cells also showed dense iron accumulation. Large neurons involved in the motor system were relatively strongly iron-positive. Subcellularly, ...
Source: Arch Histol Cytol - December 1, 2008 Category: Cytology Authors: Meguro R, Asano Y, Odagiri S, Li C, Shoumura K Tags: Arch Histol Cytol Source Type: journals

The relationship between the cusp pattern and plural stem cell compartments in guinea pig cheek teeth by chasing BrdU-labeling.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Continuously growing rodent incisors have a special epithelial structure for maintaining adult stem cells that shows a bulbous epithelial protrusion at the apical end and is referred to as an "apical bud". Guinea pig cheek teeth (premolars and molars), also continuously growing teeth, have a complex crown shape consisting of plural cusps. The present study clarifies the existence of apical buds in guinea pig premolars/molars as it examines the relationship between the crown shape and the orientation of the apical buds by micro-computed tomography (mu-CT) and immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU). One ...
Source: Arch Histol Cytol - December 1, 2008 Category: Cytology Authors: Hashimoto E, Nakakura-Ohshima K, Kenmotsu S, Suzuki H, Nakasone N, Saito C, Harada H, Ohshima H Tags: Arch Histol Cytol Source Type: journals

Comparison between a weight compression and a magnet compression for experimental pressure ulcers in the rat. Histological studies and effects of anesthesia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To develop an experimental model and evaluate the effects of the magnitude and duration of pressure, the rat abdominal wall (25 x 20 mm) was subjected to compression either by a weight or by two magnets. In the weight compression tests, a steel plate was inserted under anesthesia into the rat peritoneal cavity, and the abdominal wall was compressed in situ between the underlying steel plate and a weight placed on the abdominal wall. This method resulted in moderate changes in the subcutaneous connective tissue and muscle at 100 mmHg (13.3 kPa) for 4 h, while some muscle damage was observed at 50 mmHg (6.7 kPa) for 4 h ...
Source: Arch Histol Cytol - December 1, 2008 Category: Cytology Authors: Hashimoto M, Kurose T, Kawamata S Tags: Arch Histol Cytol Source Type: journals

Regional differences in 5-HT receptors in cerebral and testicular arterioles of the rat as revealed by Ca(2+) imaging of real-time confocal microscopy: variances by artery size and organ specificity.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
5-hydroxytriptamine (5-HT) is an important transmitter for vessel constriction. The present study was performed to clarify the effect of 5-HT on smooth muscles in large- and small-sized cerebral and testicular arterioles by confocal microscopy, with special reference to intracellular Ca(2+) concentration ([Ca(2+)](i)) dynamics. In cerebral vessels, 5-HT induced a [Ca(2+)](i) increase and the contraction of smooth muscle cells in large- and midsized arterioles (external diameters >50 mum) but not in small-sized arterioles. Conspicuous [Ca(2+)](i) changes by 5-HT were especially observed in the portions close to the c...
Source: Arch Histol Cytol - December 1, 2008 Category: Cytology Authors: Masu K, Saino T, Kuroda T, Matsuura M, Russa AD, Ishikita N, Satoh Y Tags: Arch Histol Cytol Source Type: journals

Histochemical and immunohistochemical characterization of exocrine cells in the foregut of the red-eared slider turtle, Trachemys scripta (Emydidae).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The morphofunctional organization of the exocrine cells in the foregut of the red-eared slider turtle, Trachemys scripta, was investigated by histochemistry (PAS, AB pH1.0 and pH 2.5, HID-AB, Bowie), lectin-histochemistry (WGA, SBA, UEA, ConA, PNA, DBA, sialidase-SBA, sialidase-PNA, Paradoxical ConA), and immunohistochemistry (antipepsin, anti-alpha-H(+), K(+) ATPase) to detect regional differences and verify the existence of an oro-aboral gradient in gastric juice secretion. Observations showed that pharyngeal goblet cells have mucins with terminal residuals of GalNAc and sialic acid. In the oesophagus, sulphomucins i...
Source: Arch Histol Cytol - December 1, 2008 Category: Cytology Authors: Scillitani G, Liquori GE, Mastrodonato M, Ferri D Tags: Arch Histol Cytol Source Type: journals

Regional differences in 5-HT receptors in cerebral and testicular arterioles of the rat as revealed by Ca2+ imaging of real-time confocal microscopy: variances by artery size and organ specificity.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
5-hydroxytriptamine (5-HT) is an important transmitter for vessel constriction. The present study was performed to clarify the effect of 5-HT on smooth muscles in large- and small-sized cerebral and testicular arterioles by confocal microscopy, with special reference to intracellular Ca2+ concentration ([Ca2+]i) dynamics. In cerebral vessels, 5-HT induced a [Ca2+]i increase and the contraction of smooth muscle cells in large- and midsized arterioles (external diameters>50 microm) but not in small-sized arterioles. Conspicuous [Ca2+]i changes by 5-HT were especially observed in the portions close to the cerebral arte...
Source: Arch Histol Cytol - December 1, 2008 Category: Cytology Authors: Masu K, Saino T, Kuroda T, Matsuura M, Russa AD, Ishikita N, Satoh Y Tags: Arch Histol Cytol Source Type: journals

Transvascular accumulation of Sialyl Lewis X conjugated liposome in inflamed joints of collagen antibody-induced arthritic (CAIA) mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of the current study was to investigate the specific accumulation of the Sialyl Lewis X (SLX) liposome in inflammation in the collagen-antibody induced arthritic (CAIA) model mice. The SLX-liposome encapsulating fluorescent substance (Cy5.5 or Cy3) was prepared for this study. The SLX-liposome was administered intravenously via the mouse caudal vein. After 1 to 24 h, the accumulation of SLX-liposome was observed using in vivo fluorescent imaging equipment (eXplore Optix), or the knee joints were removed for histological analysis. The in vivo fluorescent imaging showed that the signal was confined to the inflamm...
Source: Arch Histol Cytol - October 1, 2008 Category: Cytology Authors: Minaguchi J, Oohashi T, Inagawa K, Ohtsuka A, Ninomiya Y Tags: Arch Histol Cytol Source Type: journals

Type IV collagen alpha chains of the basement membrane in the rat bronchioalveolar transitional segment.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study, we have analyzed the alpha(IV) chain distribution in the subepithelial basement membrane (BM) of the rat pulmonary airway from the bronchi to alveoli. We have furthermore analyzed the alpha(IV) chain distribution in the subepithelial BM of the bronchioalveolar duct junction (BADJ) using alpha(IV) chain specific monoclonal antibodies. Our results show that the BM of the bronchial and bronchiolar epithelium contains [alpha1(IV)](2)alpha2(IV) and [alpha5(IV)](2)alpha6(IV) molecules and confirmed that the alveolar BM consists of [alpha1(IV)](2)alpha2(IV) and alpha3(IV) alpha4(IV)alpha5(IV) molecules. ...
Source: Arch Histol Cytol - October 1, 2008 Category: Cytology Authors: Hinenoya N, Naito I, Momota R, Sado Y, Kumagishi K, Ninomiya Y, Ohtsuka A Tags: Arch Histol Cytol Source Type: journals

Immunohistochemical localization of protease-activated receptors in cerebral and testicular arterioles of rats: their dependence on arteriole size and organ-specificity.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, size-dependent and/or organ-specific responses of arterioles to proteases are due to the heterogeneous localization of PARs. PMID: 19194040 [PubMed - in process]
Source: Arch Histol Cytol - October 1, 2008 Category: Cytology Authors: Misaki T, Satoh Y, Saino T, Kuroda T, Masu K, Russa DA, Ogawa A Tags: Arch Histol Cytol Source Type: journals

The inhibition of apoptosis by glycyrrhizin in hepatic injury induced by injection of lipopolysaccharide / D-galactosamine in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The inhibition of apoptosis by glycyrrhizin (GL) in hepatic injury induced by injection of lipopolysaccharide (LPS)/D-galactosamine (D-GalN) was examined in the present study. Morphological and biochemical analyses of LPS/D-GalN-induced mouse liver injury revealed that apoptosis occurred exclusively in injured hepatocytes of the centrilobular area. The degree of hepatic injury was associated with a substantial number of hepatocytes undergoing apoptosis. Transaminase levels were significantly increased at 6 to 8 h after the injection of LPS/D-GalN compared with controls. GL inhibited the elevation of serum transaminase ...
Source: Arch Histol Cytol - October 1, 2008 Category: Cytology Authors: Ikeda T, Abe K, Kuroda N, Kida Y, Inoue H, Wake K, Morito M, Sato T Tags: Arch Histol Cytol Source Type: journals

Surface morphology of the central macrophages of erythroblastic islets in the spleen of aged and pregnant mice: an immunohistochemical light microscopic study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study used 100-mum thick paraffin sections stained by the ER-HR3 antibody to examine the three-dimensional surface morphology of the central macrophages of erythroblastic islets in the splenic red pulp of aged and pregnant mice. The ER-HR3-positive cells were the macrophages located at the center of the erythroblastic islets, and the number per unit of splenic area was almost constant until 30 days of age, thereafter showing a marked decrease. In pregnant females, the ER-HR3-positive macrophage number significantly increased and became approximately eight times higher than the control value. In aged virgin females, th...
Source: Arch Histol Cytol - October 1, 2008 Category: Cytology Authors: Sonoda Y, Sasaki K Tags: Arch Histol Cytol Source Type: journals

Differential expression and localization of connexins 26 and 43 in the rat gingival epithelium.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We investigated the expression and localization of connexins (CX) 26 and 43 in the rat gingival epithelium. RT-PCR analysis revealed CX26 gene expression in both the upper and lower layers of the gingival epithelium and in the total epithelial layer, whereas CX43 gene expression was limited to the lower layer and the total epithelial layer. Immunoreactivity for CX43 was observed in the membranes of adjacent cells from the basal layer to the middle of the prickle cell layer, while immunoreactivity for CX26 was observed in the granular cell layer and lower part of the squamous cell layer. Merged images revealed the co-lo...
Source: Arch Histol Cytol - October 1, 2008 Category: Cytology Authors: Muramatsu T, Uekusa T, Masaoka T, Saitoh M, Hashimoto S, Abiko Y, Jung HS, Shimono M Tags: Arch Histol Cytol Source Type: journals

Inhibition of the terminal differentiation of odontoblasts and their transdifferentiation into osteoblasts in Runx2 transgenic mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the expression of dentin matrix protein genes, Col1a1 and dentin sialophosphoprotein (DSPP), by in situ hybridization, and dentin matrix proteins, osteocalcin, osteopontin, and dentin matrix protein 1 (DMP1) as well as an intermediate filament, nestin, by immunohistochemistry to characterize odontoblasts in Tg(Col1a1-Runx2) mice. Results showed Col1a1 expression was down-regulated, DSPP expression was lost, and nestin expression was severely decreased in the odontoblasts of Tg(Col1a1-Runx2) mice. Further, the expressions of osteocalcin, osteopontin, and DMP1 were up-regulated in odontoblasts, although the up-re...
Source: Arch Histol Cytol - June 1, 2008 Category: Cytology Authors: Miyazaki T, Kanatani N, Rokutanda S, Yoshida C, Toyosawa S, Nakamura R, Takada S, Komori T Tags: Arch Histol Cytol Source Type: journals

The serine racemase mRNA is expressed in both neurons and glial cells of the rat retina.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
D-Serine, an endogenous and obligatory coagonist for the glycine site of the N-methyl-D-aspartate receptor in mammals, is synthesized from L-serine by serine racemase. Serine racemase and D-serine have long been believed to occur predominantly in astrocytes, according to immunohistochemical studies. Recent studies have demonstrated, however, that both the mRNA and protein levels of serine racemase are considerably higher in neurons than in astrocytes in primary cultures of the rat brain and that the mRNA level of serine racemase predominates in neurons of the adult rat brain. Here we report the application of in situ h...
Source: Arch Histol Cytol - June 1, 2008 Category: Cytology Authors: Takayasu N, Yoshikawa M, Watanabe M, Tsukamoto H, Suzuki T, Kobayashi H, Noda S Tags: Arch Histol Cytol Source Type: journals

An electron microscopic study on nerve endings on adrenomedullary adrenaline cells in golden hamsters: position, size and changes due to pinealectomy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, preganglionic nerve endings in the adrenal medulla of the golden hamster show differential morphological changes following PX depending on the intracellular part of A cells. PMID: 18974603 [PubMed - in process]
Source: Arch Histol Cytol - June 1, 2008 Category: Cytology Authors: Yamauchi T, Kachi T Tags: Arch Histol Cytol Source Type: journals

Chelatable Fe (II) is generated in the rat kidneys exposed to ischemia and reperfusion, and a divalent metal chelator, 2, 2'-dipyridyl, attenuates the acute ischemia/reperfusion-injury of the kidneys: a histochemical study by the perfusion-Perls and -Turnbull methods.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The perfusion-Perls and -Turnbull methods supplemented by diaminobenzidine intensification demonstrated the generation and localization of chelatable Fe (II) which can catalyze the generation of cytotoxic hydroxyl radicals (OH.) during the Fenton reaction in rat kidneys exposed to 40 min ischemia or 40 min-ischemia followed by 60 min-reperfusion. The kidneys exposed to 40 min-ischemia showed Fe (II)-deposits largely localized in the deeper half of the cortex, where the deposits densely filled the tubular cell nuclei, with a small amount of them in the cytoplasm of the proximal convoluted tubules (PCT). Intraluminally p...
Source: Arch Histol Cytol - June 1, 2008 Category: Cytology Authors: Iwatsuki H, Meguro R, Asano Y, Odagiri S, Li C, Shoumura K Tags: Arch Histol Cytol Source Type: journals

Comparison of expression patterns of cathepsin K and MMP-9 in odontoclasts and osteoclasts in physiological root resorption in the rat molar.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Root resorption lacunae are principally formed by odontoclasts. While these cells develop from the same origin as osteoclasts, odontoclasts normally have fewer nuclei and a less clear zone compared with osteoclasts. We therefore, hypothesized that odontoclasts possess less differentiation in matrix resorption characteristics than osteoclasts. To test our hypothesis, we compared the TRAP-positive area and the expression patterns of two important proteolytic enzymes, cathepsin K and matrix metalloproteinase-9 (MMP-9), between odontoclasts and osteoclasts. We focused on physiological root resorption in the rat molar, whic...
Source: Arch Histol Cytol - June 1, 2008 Category: Cytology Authors: Tsuchiya M, Akiba Y, Takahashi I, Sasano Y, Kashiwazaki J, Tsuchiya S, Watanabe M Tags: Arch Histol Cytol Source Type: journals

Endothelin A receptor-like immunoreactivity on the basal infoldings of rat renal tubules and collecting ducts.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We investigated the distribution of endothelin A (ET(A)) receptor-like immunoreactivity in the rat kidney using affinity-purified antibodies against amino acid residues 403-417 of the rat ET(A) receptor modified by the multiple antigen peptide complex system. Western blot analysis using the affinity-purified anti-ET(A) antibody detected bands of approximately 47.3 and 64.5 kDa in the rat kidney. By light microscopy, ET(A) receptor-like immunoreactivity was seen in the basal side of the renal tubules and collecting ducts. The most intense immunoreactivity was present in the distal renal tubules and inner medullary colle...
Source: Arch Histol Cytol - June 1, 2008 Category: Cytology Authors: Yamamoto T, Suzuki H, Kubo Y, Matsumoto A, Uemura H Tags: Arch Histol Cytol Source Type: journals

Structure and function of rat lymph nodes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The lymph node comprises a critical crossroad for encounters between antigen presenting cells, antigens from lymph, and lymphocytes recruited into lymph nodes from the blood. The node consists of spaces lined with lymphatic endothelial cells and parenchyma. The former spaces can be divided into the subcapsular sinuses, lymphatic labyrinths in the deep cortex, intermediate sinuses, and medullary sinuses. The sponge-like framework of the node parenchyma is composed of collagen fibers invested with reticular cells. The parenchyma can be divided into the cortex, deep cortex, and medullary cord. Lymphocytes migrate from the...
Source: Arch Histol Cytol - June 1, 2008 Category: Cytology Authors: Ohtani O, Ohtani Y Tags: Arch Histol Cytol Source Type: journals

Cell-type specific occurrence of apoptosis in taste buds of the rat circumvallate papilla.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study employed immunohistochemistry for single-stranded DNA (ssDNA) to detect apoptotic cells in taste buds of the rat circumvallate papilla. Double-labeling of ssDNA and markers for each cell type - phospholipase C beta2 (PLCbeta2) and alpha-gustducin for type II cells, neural cell adhesion molecule (NCAM) for type III cells, and Jacalin for type IV cells - was also performed to reveal which types of cells die by apoptosis. We detected approximately 16.8% and 14.0% of ssDNA-immunoreactive nuclei among PLCbeta2-immunoreactive and alpha-gustducinimmunoreactive cells, respectively, but rarely found ssDNA-immu...
Source: Arch Histol Cytol - May 1, 2008 Category: Cytology Authors: Ueda K, Ichimori Y, Maruyama H, Murakami Y, Fujii M, Honma S, Wakisaka S Tags: Arch Histol Cytol Source Type: journals

The skeletal muscle vascular supply closely correlates with the muscle fiber surface area in the rat.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, capillarity was closely correlated with the muscle fiber radius (which represents the perimeter) during growth and atrophy. This indicates that the capillarity is linked to the muscle fiber surface area (which is determined by perimeter and section thickness), in agreement with the essential role of the cell membrane in the transport of materials by simple diffusion or active transport. PMID: 18622093 [PubMed - in process]
Source: Arch Histol Cytol - May 1, 2008 Category: Cytology Authors: Ichinose E, Kurose T, Daitoku D, Kawamata S Tags: Arch Histol Cytol Source Type: journals

The modulation of collagen fibril assembly and its structure by decorin: an electron microscopic study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, this in vitro study showed that decorin is a regulatory and architecturally small leucine-rich repeat proteoglycan in the process of collagen fibril assembly. PMID: 18622092 [PubMed - in process]
Source: Arch Histol Cytol - May 1, 2008 Category: Cytology Authors: Iwasaki S, Hosaka Y, Iwasaki T, Yamamoto K, Nagayasu A, Ueda H, Kokai Y, Takehana K Tags: Arch Histol Cytol Source Type: journals

Appearance of LFA-1 in the initial stage of synaptogenesis of developing hippocampal neurons.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study, we found that leucocyte function-associated antigen-1 (LFA-1), and integrin (heterodimer complex of CD11a and CD18), which are abundant in immunological synapse, were expressed in developing hippocampal neurons. The expression of LFA-1 in hippocampal neurons was in the period of synaptogenesis, and synaptogenesis was inhibited by the blocking antibodies of anti-CD11a or anti-CD18 in vitro. Since it is known that LFA-1 has an important role in the immunological response, especially in immunological synapse, LFA-1 is considered to have an important role in neuronal synapse and is highly involved in ...
Source: Arch Histol Cytol - May 1, 2008 Category: Cytology Authors: Wakabayashi Y, Tsujimura A, Matsuda K, Yoshimura N, Kawata M Tags: Arch Histol Cytol Source Type: journals

Organization and developmental aspects of lymphatic vessels.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The lymphatic system plays important roles in maintaining tissue fluid homeostasis, immune surveillance of the body, and the taking up dietary fat and fat-soluble vitamins A, D, E and K. The lymphatic system is involved in many pathological conditions, including lymphedema, inflammatory diseases, and tumor dissemination. A clear understanding of the organization of the lymphatic vessels in normal conditions would be critically important to develop new treatments for diseases involving the lymphatic vascular system. Therefore, the present paper reviews the organization of the lymphatic vascular system of a variety of or...
Source: Arch Histol Cytol - May 1, 2008 Category: Cytology Authors: Ohtani O, Ohtani Y Tags: Arch Histol Cytol Source Type: journals

The differential distribution of type IV collagen alpha chains in the subepithelial basement membrane of the human alimentary canal.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We studied distribution patterns of type IV collagen alpha chains in the subepithelial basement membrane (SBM) of the human gastrointestinal tract - the esophagus through the anal canal - by immunofluorescent microscopy using alpha(IV) chain-specific monoclonal antibodies. The alpha1(IV), alpha2(IV), alpha5(IV), and alpha6(IV) chains were found in the SBM throughout the tract, indicating the localization of [alpha1(IV)](2)alpha2(IV) and [alpha5(IV)](2)alpha6(IV) heterotrimeric molecules. The [alpha1(IV)](2)alpha2(IV) molecule was continuously stained, while the [alpha5(IV)](2)alpha6(IV) molecule was weakly stained in g...
Source: Arch Histol Cytol - April 1, 2008 Category: Cytology Authors: Sato H, Naito I, Momota R, Naomoto Y, Yamatsuji T, Sado Y, Ninomiya Y, Ohtsuka A Tags: Arch Histol Cytol Source Type: journals

Specific depletion of GGA2 causes cathepsin D missorting in HeLa cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Three mammalian GGAs (Golgi-localized, gamma-ear-containing, ARF-binding proteins), GGA1, 2, and 3 have been implicated in the sorting of mannose 6-phosphate receptor (MPR). To investigate the distinct roles of GGA2 in lysosomal enzyme transport, we established two stable cell lines that had a reduced expression of GGA2 by RNA interference. The expression levels of GGA2 were approximately 5% of the control levels, whereas those of non-targeted GGA1 and GGA3 were not apparently reduced. The depletion of GGA2 did not cause changes in the overall distribution of GGA1, GGA3, cation-dependent MPR, or cation-independent MPR....
Source: Arch Histol Cytol - April 1, 2008 Category: Cytology Authors: Hida T, Ikeda H, Kametaka S, Akazawa C, Kohsaka S, Ebisu S, Uchiyama Y, Waguri S Tags: Arch Histol Cytol Source Type: journals

Anterograde labeling of the corticospinal tract in jimpy mutant mice by Di I injection into the motor cortex.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Carbocyanine fluorescent dye, DiI, is an excellent anterograde/retrograde neural tracer, but its efficacy for the anterograde labeling of neural circuits in the adult brain tends to decrease with ages. The present study shows that an injection of DiI into the motor cortex of the young adult jimpy mutant mice (Plp1(jp)/+) resulted in successful anterograde labeling of corticospinal tract fibers. Furthermore, an injection of Fast Blue into the lumbar spinal cord of the mutant mice resulted in retrograde labeling of layer 5 corticospinal tract neurons within the motor cortex. Since no abnormality except for myelin deficie...
Source: Arch Histol Cytol - April 1, 2008 Category: Cytology Authors: Shibata-Iwasaki R, Dekimoto H, Katsuyama Y, Kikkawa S, Terashima T Tags: Arch Histol Cytol Source Type: journals

Existence of subtypes of gustducin-immunoreactive cells in the vallate taste bud of guinea pigs.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Vallate taste buds in the guinea-pig tongue were immunohistochemically investigated with regard to the colocalization of gustducin with calbindin-D28K (=spot 35 protein) and type III inositol triphosphate receptor (IP(3)R-3) in order to characterize gustducin-immunoreactive cells. Individual taste bud cells ranged from totally immunopositive to totally immunonegative for these three molecules. Among the immunoreactive cells, gustducin-immunoreactive cells were divided into two cell populations: one immunopositive and the other immunonegative for calbindin-D28K. Applying our previous data to the present results, the for...
Source: Arch Histol Cytol - April 1, 2008 Category: Cytology Authors: Ohkubo Y, Yokosuka H, Kumakura M, Yoshie S Tags: Arch Histol Cytol Source Type: journals

Reduced expression of endogenous secretory receptor for advanced glycation endproducts in hippocampal neurons of Alzheimer's disease brains.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the expression of esRAGE in the hippocampal tissues from AD brains compared with control (non-AD) was examined by immunohistochemistry and Western blot analysis. Semiquantitative immunohistochemical analysis of hippocampal tissues using esRAGE-specific antibody revealed significantly decreased immunoreactivities in pyramidal cells in CA1 and CA3 regions of AD compared with non-AD. On the other hand, immunoreactivities of astrocytes for esRAGE significantly increased in those regions. Dentate granule cells and astrocytes showed essentially invariant immunoreactivities between AD and non-AD. Changes in esRAGE ...
Source: Arch Histol Cytol - April 1, 2008 Category: Cytology Authors: Nozaki I, Watanabe T, Kawaguchi M, Akatsu H, Tsuneyama K, Yamamoto Y, Ohe K, Yonekura H, Yamada M, Yamamoto H Tags: Arch Histol Cytol Source Type: journals

Soluble EMMPRIN (extra-cellular matrix metalloproteinase inducer) stimulates the migration of HEp-2 human laryngeal carcinoma cells, accompanied by increased MMP-2 production in fibroblasts.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The basement membrane functions as a barrier against the invasion of cancer cells. It is therefore important to investigate the mechanism of basement membrane degradation by matrix metalloproteinases (MMPs). Previously, cancer cells were long considered to be the major source of MMPs; however, current evidence indicates that most MMPs in cancer tissue are produced by stromal rather than cancer cells. A glycoprotein highly expressed on the cancer-cell membrane, EMMPRIN (extra-cellular matrix metalloproteinase inducer), exhibits the potential role of the MMP inductor in stromal cells. Depending on the cell type, EMMPRIN ...
Source: Arch Histol Cytol - April 1, 2008 Category: Cytology Authors: Hanata K, Yamaguchi N, Yoshikawa K, Mezaki Y, Miura M, Suzuki S, Senoo H, Ishikawa K Tags: Arch Histol Cytol Source Type: journals

The distributions of type IV collagen alpha chains in basement membranes of human epidermis and skin appendages.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Distributions of type IV collagen alpha chains in the basement membrane (BM) of human skin and its appendages were analyzed by immunofluorescent microscopy using chain-specific monoclonal antibodies. The basement membrane beneath the epidermis contained [alpha1(IV)](2)alpha2(IV) and [alpha5(IV)](2)alpha6(IV) but no alpha3(IV)alpha4(IV)alpha5(IV); this held true for at the eccrine sweat glands and glandular ducts, sebaceous glands, hair follicles, and arrector muscles of hair. The secretary portion of the eccrine sweat glands was rich in [alpha1(IV)](2) alpha2(IV) and had less [alpha5(IV)](2)alpha6(IV), while [alpha5(IV...
Source: Arch Histol Cytol - December 1, 2007 Category: Cytology Authors: Hasegawa H, Naito I, Nakano K, Momota R, Nishida K, Taguchi T, Sado Y, Ninomiya Y, Ohtsuka A Tags: Arch Histol Cytol Source Type: journals

The distribution of type IV collagen alpha chains in the mouse ovary and its correlation with follicular development.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study aims to identify alpha chains of type IV collagen in the basement membrane of the mouse ovarian follicle and examine their changes during follicular development using immunofluorescence microscopy with specific monoclonal antibodies. The basement membrane of the serous mesothelium enveloping the ovary contained all alpha chains of type IV collagen, alpha1(IV) through alpha6(IV) chains. Primordial follicles showed a distinct immunoreactivity against all six alpha chains in their basement membranes. Immunolabeling for alpha3(IV) and alpha4(IV) chains was almost eliminated in the primary follicles. In ba...
Source: Arch Histol Cytol - December 1, 2007 Category: Cytology Authors: Nakano K, Naito I, Momota R, Sado Y, Hasegawa H, Ninomiya Y, Ohtsuka A Tags: Arch Histol Cytol Source Type: journals

Distinct morphology of serotonin-containing enterochromaffin (EC) cells in the rat distal colon.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study was performed to examine the distribution and distinct morphology of the serotonin-containing enterochromaffin (EC) cells in the rat distal colon by immunohistochemical and electron microscopic methods. Serotonin-immunohistochemistry revealed that most of the serotonin-immunoreactive EC cells possessed extended cytoplasmic processes. In particular, the immunoreactive EC cells with long processes located along the body of the crypt were characterized by their bipolar processes comprising one with the terminal swellings extending vertically down to the basal crypt and the other running up along the lumi...
Source: Arch Histol Cytol - December 1, 2007 Category: Cytology Authors: Kuramoto H, Kadowaki M, Sakamoto H, Yuasa K, Todo A, Shirai R Tags: Arch Histol Cytol Source Type: journals

The distribution of proliferating cell nuclear antigen-immunoreactive cells in the pineal organ of the rainbow trout Oncorhynchus mykiss.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cell proliferation in the pineal organ of the immature rainbow trout Oncorhynchus mykiss was investigated by immunocytochemical demonstration of the proliferating cell nuclear antigen (PCNA) together with photoreceptor-specific opsin. Numerous PCNA-immunoreactive cells were found throughout the pineal end-vesicle and stalk. Two types of PCNA-immuno-reactive cells were distinguished: intensely stained, large ovoid and round cells, and mildly stained, slender fusiform cells. The ovoid type of the former cell was found often in the apical region and the round type in the basal region of the epithelium, while the latter fu...
Source: Arch Histol Cytol - December 1, 2007 Category: Cytology Authors: Omura Y Tags: Arch Histol Cytol Source Type: journals

Immunohistochemical identification of cells expressing steroidogenic enzymes cytochrome P450scc and P450 aromatase in taste buds of rat circumvallate papillae.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study demonstrated for the first time the localizations and patterns of expression of key enzymes for steroidogenesis, cytochrome P450 side-chain-cleavage (P450scc), and P450 aromatase in the taste buds of rat circumvallate papillae, using immunoblot analyses and immunohistochemistry. Immunoblot analyses showed that proteins with a molecular weight close to that of rat adrenal cytochrome P450scc and a molecular weight close to that of rat ovary cytochrome P450 aromatase were present in the rat circumvallate papillae. In immunohistochemistry, antibodies against cytochrome P450scc and P450 aromatase yielded t...
Source: Arch Histol Cytol - December 1, 2007 Category: Cytology Authors: Toyoshima K, Seta Y, Toyono T, Kataoka S Tags: Arch Histol Cytol Source Type: journals

A possible monosynaptic pathway links the pedunculopontine tegmental nucleus to thalamostriatal neurons in the hooded rat.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The thalamic lateral posterior nucleus (LP) of the hooded rat is regarded as a relay nucleus for the transmission of information from visuomotor-related structures such as the superior colliculus, pedunculopontine tegmental nucleus (PPT) and substantia nigra, pars reticulata, to visual cortical areas as well as the striatum. The aim of the present study was to examine the relationships of the thalamo-striatal projection neurons with the LP afferent fibers derived from the PPT, using injections of the anterograde tracer biotinylated dextran amine (BDA) and the retrograde tracer cholera toxin-B (CTB) into the PPT and the...
Source: Arch Histol Cytol - October 1, 2007 Category: Cytology Authors: Kobayashi K, Hoshino K, Homma S, Takagi S, Norita M Tags: Arch Histol Cytol Source Type: journals

Hypothermic, but not normothermic, ischemia causes a drastic increase in cyclooxygenase-2 immunoreactive granule cells in rat dentate gyrus after 4 hours of ischemic reperfusion.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We investigated cyclooxygenase-2 (Cox-2) immunoreactive cells in the hippocampus and dentate gyrus of Sprague-Dawley rats at 4 h after the induction of normothermic and hypothermic ischemia and reperfusion. Under the normothermic condition, Cox-2 immunoreactive cells showed more intense staining and clearer proximal dendrite configurations as compared with the control animals, whereas the numbers of immunoreactive cells in the hippocampus and dentate gyrus were not remarkably increased. In contrast to the normothermic condition, long-term (pre- and intra-ischemic) and short-term (exclusively intra-ischemic) hypothermic...
Source: Arch Histol Cytol - October 1, 2007 Category: Cytology Authors: Yamashita A, Kunimatsu T, Yamamoto T, Yoshida K Tags: Arch Histol Cytol Source Type: journals

Immunohistochemical features of substance P-immunoreactive chromaffin cells and nerve fibers in the rat adrenal gland.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The distribution of substance P (SP) immunoreactivity and the colocalization of SP with other bioactive substances in chromaffin cells and nerve fibers were investigated in the rat adrenal gland at the light microscopic level. In the capsule and cortex, SP immunoreactivity was seen in some nerve fibers around blood vessels and in thick nerve bundles passing through the cortex directly into the medulla. In the medulla, the SP immunoreactivity was observed in a small number of chromaffin cells; these SP-immunoreactive chromaffin cells were either phenylethanolamine N-methyltransferase (PNMT) immunoreactive or immunonegat...
Source: Arch Histol Cytol - October 1, 2007 Category: Cytology Authors: Murabayashi H, Kuramoto H, Kawano H, Sasaki M, Kitamura N, Miyakawa K, Tanaka K, Oomori Y Tags: Arch Histol Cytol Source Type: journals

Age-related changes in the microarchitecture of collagen fibrils in the articular disc of the rat temporomandibular joint.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The microarchitecture of collagen fibrils in the articular disc of the temporomandibular joint (TMJ) plays an important role in dissipating the mechanical load during jaw movement. However, little information is available on its adaptations to the biomechanical environment during development. To address this issue, we analyzed the diameter of collagen fibrils of the articular disc of the rat TMJ with quantitative ultrastructural analysis during postnatal development. The mean diameter of the collagen fibrils significantly increased and the arrangement of the collagen fiber networks became compact during development. Ar...
Source: Arch Histol Cytol - October 1, 2007 Category: Cytology Authors: Ahn HJ, Paik SK, Choi JK, Kim HJ, Ahn DK, Cho YS, Kim YS, Moon C, Bae YC Tags: Arch Histol Cytol Source Type: journals

Interstitial cells of Cajal in the gastrointestinal musculature of W mutant mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Interstitial cells of Cajal (ICC) are important regulatory cells generating electrical rhythmicity and transducing neural signals in the gastrointestinal musculature. ICC express the proto-oncogene c-kit, a receptor tyrosine kinase, and can be examined morphologically using the c-Kit antibody. The c-kit gene is allelic with the murine white-spotting locus W, and the c-kit mutation (W mutation) affects various aspects of hematopoietic cells, germ cells, melanocytes, mast cells, and ICC. Heterozygous W/W( v) mutant mice lack a specific type of ICC and have been used to reveal its function. To search for a new model that ...
Source: Arch Histol Cytol - October 1, 2007 Category: Cytology Authors: Iino S, Horiguchi S, Horiguchi K, Nojyo Y Tags: Arch Histol Cytol Source Type: journals