Diagnostic investigation of Avian Reovirus Field Variants Circulating in Broiler Chickens in Pennsylvania of United States between 2017-2022
This report provides our diagnostic investigation on monitoring ARV field variants from broiler chickens in Pennsylvania. Genomic characterization findings of 72 ARV field isolates obtained from broiler cases during the last six years indicated that the six distinct cluster variant strains (genotype I-VI) continuously circulated in PA poultry, which were genetically diverse and distant from the vaccine and vaccine-related field strains. Most of the variants clustered within genotype V (24/72, 33.3%), followed by genotype II (16/72, 22.2%), genotype IV (13/72, 18.1%), genotype III (13/72, 18.1%), genotype VI (05/72, 6.94%),...
Source: Avian Pathology - April 17, 2024 Category: Pathology Authors: Muhammad Zubair Shabbir Haiyang Yu Megan E Lighty Patricia Ann Dunn Eva A Wallner-Pendleton Huaguang Lu Source Type: research

Effects of < em > Artemisia absinthium < /em > on broiler chicken coccidiosis: a systematic review and meta-analysis
This study aims to evaluate the effect of Artemisia absinthium on coccidiosis in broilers through a systematic review and meta-analysis. The article selection process included a search from the year 2000 to February 2021, with no restrictions on country or geographical region. Our objective was met by only six (06) studies, which underwent systematic review. The meta-analysis was conducted using the metafor package in R via RStudio (version 1.1.383; RStudio, Inc.).The systematic review indicates that in vivo studies have shown the effectiveness of various plant extracts (essential oil and methanolic extract) when administe...
Source: Avian Pathology - April 15, 2024 Category: Pathology Authors: Nadia Hezil Djamila Baazize-Ammi Amine Abdelli Amel Adel Seddik Kebbal Ismail Gharbi Rh éda Djezzar Djamel Guetarni Source Type: research

Effects of < em > Artemisia absinthium < /em > on broiler chicken coccidiosis: a systematic review and meta-analysis
This study aims to evaluate the effect of Artemisia absinthium on coccidiosis in broilers through a systematic review and meta-analysis. The article selection process included a search from the year 2000 to February 2021, with no restrictions on country or geographical region. Our objective was met by only six (06) studies, which underwent systematic review. The meta-analysis was conducted using the metafor package in R via RStudio (version 1.1.383; RStudio, Inc.).The systematic review indicates that in vivo studies have shown the effectiveness of various plant extracts (essential oil and methanolic extract) when administe...
Source: Avian Pathology - April 15, 2024 Category: Pathology Authors: Nadia Hezil Djamila Baazize-Ammi Amine Abdelli Amel Adel Seddik Kebbal Ismail Gharbi Rh éda Djezzar Djamel Guetarni Source Type: research

Extensive genetic and biological characterization of infectious bronchitis virus strain D2860 of genotype GVIII
Avian Pathol. 2024 Apr 15:1-10. doi: 10.1080/03079457.2024.2338801. Online ahead of print.ABSTRACTInfectious bronchitis virus (IBV) strains of genotype GVIII have been emerging in Europe in the last decade, but no biological characterization has been reported so far. This paper reports the extensive genetic and biological characterization of IBV strain D2860 of genotype GVIII which was isolated from a Dutch layer flock that showed a drop in egg production. Whole genome sequencing showed that it has a high similarity (95%) to CK/DE/IB80/2016 (commonly known as IB80). Cross-neutralization tests with antigens and serotype-spe...
Source: Avian Pathology - April 4, 2024 Category: Pathology Authors: R J Molenaar R Dijkman I Jorna J J de Wit Source Type: research

Extensive genetic and biological characterization of infectious bronchitis virus strain D2860 of genotype GVIII
Avian Pathol. 2024 Apr 4:1-17. doi: 10.1080/03079457.2024.2338801. Online ahead of print.ABSTRACTInfectious bronchitis virus (IBV) strains of genotype GVIII have been emerging in Europe in the last decade, but no biological characterisation has been reported so far. This paper reports the extensive genetic and biological characterization of IBV strain D2860 of genotype GVIII that was isolated from a Dutch layer flock that showed a drop in egg production. Whole genome sequencing showed that it has a high similarity (95%) to CK/DE/IB80/2016 (commonly known as IB80). Cross-neutralization tests with antigens and serotype-speci...
Source: Avian Pathology - April 4, 2024 Category: Pathology Authors: R J Molenaar R Dijkman I Jorna J J de Wita Source Type: research

Extensive genetic and biological characterization of infectious bronchitis virus strain D2860 of genotype GVIII
Avian Pathol. 2024 Apr 4:1-17. doi: 10.1080/03079457.2024.2338801. Online ahead of print.ABSTRACTInfectious bronchitis virus (IBV) strains of genotype GVIII have been emerging in Europe in the last decade, but no biological characterisation has been reported so far. This paper reports the extensive genetic and biological characterization of IBV strain D2860 of genotype GVIII that was isolated from a Dutch layer flock that showed a drop in egg production. Whole genome sequencing showed that it has a high similarity (95%) to CK/DE/IB80/2016 (commonly known as IB80). Cross-neutralization tests with antigens and serotype-speci...
Source: Avian Pathology - April 4, 2024 Category: Pathology Authors: R J Molenaar R Dijkman I Jorna J J de Wita Source Type: research

Extensive genetic and biological characterization of infectious bronchitis virus strain D2860 of genotype GVIII
Avian Pathol. 2024 Apr 4:1-17. doi: 10.1080/03079457.2024.2338801. Online ahead of print.ABSTRACTInfectious bronchitis virus (IBV) strains of genotype GVIII have been emerging in Europe in the last decade, but no biological characterisation has been reported so far. This paper reports the extensive genetic and biological characterization of IBV strain D2860 of genotype GVIII that was isolated from a Dutch layer flock that showed a drop in egg production. Whole genome sequencing showed that it has a high similarity (95%) to CK/DE/IB80/2016 (commonly known as IB80). Cross-neutralization tests with antigens and serotype-speci...
Source: Avian Pathology - April 4, 2024 Category: Pathology Authors: R J Molenaar R Dijkman I Jorna J J de Wita Source Type: research

Extensive genetic and biological characterization of infectious bronchitis virus strain D2860 of genotype GVIII
Avian Pathol. 2024 Apr 4:1-17. doi: 10.1080/03079457.2024.2338801. Online ahead of print.ABSTRACTInfectious bronchitis virus (IBV) strains of genotype GVIII have been emerging in Europe in the last decade, but no biological characterisation has been reported so far. This paper reports the extensive genetic and biological characterization of IBV strain D2860 of genotype GVIII that was isolated from a Dutch layer flock that showed a drop in egg production. Whole genome sequencing showed that it has a high similarity (95%) to CK/DE/IB80/2016 (commonly known as IB80). Cross-neutralization tests with antigens and serotype-speci...
Source: Avian Pathology - April 4, 2024 Category: Pathology Authors: R J Molenaar R Dijkman I Jorna J J de Wita Source Type: research

Extensive genetic and biological characterization of infectious bronchitis virus strain D2860 of genotype GVIII
Avian Pathol. 2024 Apr 4:1-17. doi: 10.1080/03079457.2024.2338801. Online ahead of print.ABSTRACTInfectious bronchitis virus (IBV) strains of genotype GVIII have been emerging in Europe in the last decade, but no biological characterisation has been reported so far. This paper reports the extensive genetic and biological characterization of IBV strain D2860 of genotype GVIII that was isolated from a Dutch layer flock that showed a drop in egg production. Whole genome sequencing showed that it has a high similarity (95%) to CK/DE/IB80/2016 (commonly known as IB80). Cross-neutralization tests with antigens and serotype-speci...
Source: Avian Pathology - April 4, 2024 Category: Pathology Authors: R J Molenaar R Dijkman I Jorna J J de Wita Source Type: research

Extensive genetic and biological characterization of infectious bronchitis virus strain D2860 of genotype GVIII
Avian Pathol. 2024 Apr 4:1-17. doi: 10.1080/03079457.2024.2338801. Online ahead of print.ABSTRACTInfectious bronchitis virus (IBV) strains of genotype GVIII have been emerging in Europe in the last decade, but no biological characterisation has been reported so far. This paper reports the extensive genetic and biological characterization of IBV strain D2860 of genotype GVIII that was isolated from a Dutch layer flock that showed a drop in egg production. Whole genome sequencing showed that it has a high similarity (95%) to CK/DE/IB80/2016 (commonly known as IB80). Cross-neutralization tests with antigens and serotype-speci...
Source: Avian Pathology - April 4, 2024 Category: Pathology Authors: R J Molenaar R Dijkman I Jorna J J de Wita Source Type: research

Extensive genetic and biological characterization of infectious bronchitis virus strain D2860 of genotype GVIII
Avian Pathol. 2024 Apr 4:1-17. doi: 10.1080/03079457.2024.2338801. Online ahead of print.ABSTRACTInfectious bronchitis virus (IBV) strains of genotype GVIII have been emerging in Europe in the last decade, but no biological characterisation has been reported so far. This paper reports the extensive genetic and biological characterization of IBV strain D2860 of genotype GVIII that was isolated from a Dutch layer flock that showed a drop in egg production. Whole genome sequencing showed that it has a high similarity (95%) to CK/DE/IB80/2016 (commonly known as IB80). Cross-neutralization tests with antigens and serotype-speci...
Source: Avian Pathology - April 4, 2024 Category: Pathology Authors: R J Molenaar R Dijkman I Jorna J J de Wita Source Type: research

Extensive genetic and biological characterization of infectious bronchitis virus strain D2860 of genotype GVIII
Avian Pathol. 2024 Apr 4:1-17. doi: 10.1080/03079457.2024.2338801. Online ahead of print.ABSTRACTInfectious bronchitis virus (IBV) strains of genotype GVIII have been emerging in Europe in the last decade, but no biological characterisation has been reported so far. This paper reports the extensive genetic and biological characterization of IBV strain D2860 of genotype GVIII that was isolated from a Dutch layer flock that showed a drop in egg production. Whole genome sequencing showed that it has a high similarity (95%) to CK/DE/IB80/2016 (commonly known as IB80). Cross-neutralization tests with antigens and serotype-speci...
Source: Avian Pathology - April 4, 2024 Category: Pathology Authors: R J Molenaar R Dijkman I Jorna J J de Wita Source Type: research

Identification of B cell epitopes located on the surface in PB2 protein of H9N2 subtype avian influenza virus
In this study, PB2 sequence of H9N2 subtype AIV from 1090 to 1689 bp was cloned and expressed in the prokaryotic expression pET-28a vector. After purified, the recombinant PB2 protein with cutting gel was used to immunize BALB/c mice. After cell fusion, the Hybridoma cell lines secreting monoclonal antibodies (mAbs) targeting to PB2 protein were screened by indirect ELISA and Western blotting, and the antigenic epitopes of mAbs were identified by constructing truncated overlapping fragments in PB2 protein of H9N2 subtype AIV. The results showed that three hybridoma cell lines (4B7, 4D10, 5H1) that stably secreted mAbs spec...
Source: Avian Pathology - April 2, 2024 Category: Pathology Authors: Yiqin Cai Guihu Yin Xiangyu Huang Jianing Hu Zichen Gao Xinyu Guo Yawei Qiu Haifeng Sun Xiuli Feng Source Type: research

Identification of B cell epitopes located on the surface in PB2 protein of H9N2 subtype avian influenza virus
In this study, PB2 sequence of H9N2 subtype AIV from 1090 to 1689 bp was cloned and expressed in the prokaryotic expression pET-28a vector. After purified, the recombinant PB2 protein with cutting gel was used to immunize BALB/c mice. After cell fusion, the Hybridoma cell lines secreting monoclonal antibodies (mAbs) targeting to PB2 protein were screened by indirect ELISA and Western blotting, and the antigenic epitopes of mAbs were identified by constructing truncated overlapping fragments in PB2 protein of H9N2 subtype AIV. The results showed that three hybridoma cell lines (4B7, 4D10, 5H1) that stably secreted mAbs spec...
Source: Avian Pathology - April 2, 2024 Category: Pathology Authors: Yiqin Cai Guihu Yin Xiangyu Huang Jianing Hu Zichen Gao Xinyu Guo Yawei Qiu Haifeng Sun Xiuli Feng Source Type: research

The financial cost of coccidiosis in Algerian chicken production: A major challenge for the poultry sector
In this study, we conducted a comprehensive analysis to evaluate the financial losses associated with Eimeria infection in chickens in Algeria, relying on data provided by key stakeholders in the Algerian poultry industry to assess sub-clinical as well as clinical impact. The updated 2020 version of a model established to estimate the cost of coccidiosis in chickens was employed, taking into consideration specific cultural and technical aspects of poultry farming in Algeria.The findings predict economic losses due to coccidiosis in chickens of approximately UK £86.7 million in Algeria for the year 2022, representing UK £...
Source: Avian Pathology - March 26, 2024 Category: Pathology Authors: Abderrahmen Rahmani Hamza Ahmed Laloui Radhouane Kara Mohamed Abdesselem Dems Nora Cherb Abdenour Klikha Damer P Blake Source Type: research