Time resolved amplified FRET identifies protein kinase B activation state as a marker for poor prognosis in clear cell renal cell carcinoma
Conclusions Quantitative determination of PKB/Akt activation in ccRCC primary tumours alongside other diagnostics tools could prove key in taking oncologists closer to an efficient personalised therapy in ccRCC patients. General significance The quantitative imaging technology based on Amplified-FRET can rapidly analyse protein activation states and molecular interactions. It could be used for prognosis and assess drug function during the early cycles of chemotherapy. It enables evaluation of clinical efficiency of personalised cancer treatment. (Source: BBA Clinical)
Source: BBA Clinical - October 14, 2017 Category: Biochemistry Source Type: research
Multiple exo-glycosidases in human serum as detected with the substrate DNP- α-GalNAc. I. A new assay for lysosomal α-N-acetylgalactosaminidase
Publication date: Available online 7 October 2017 Source:BBA Clinical Author(s): Simon P.J. Albracht, Erik Allon, Johannes van Pelt This paper presents a new assay to determine the activity of the lysosomal enzyme α-N-acetylgalactosaminidase (Naga, EC 3.2.1.49) in human serum. It is based on the use of a new chromogenic substrate, DNP-α-GalNAc (2,4-dinitrophenyl-N-acetyl-α-D-galactosaminide) and is performed at pH4.3 and 37°C. This allows continuous monitoring of the absorbance of the released DNP. The assay can be performed with a standard spectrophotometer. Compared to established methods using an endpoint assay...
Source: BBA Clinical - October 7, 2017 Category: Biochemistry Source Type: research
Multiple exo-glycosidases in human serum as detected with the substrate DNP- α-GalNAc. II. Three α-N-acetylgalactosaminidase-like activities in the pH5 to 8 region
Publication date: Available online 22 September 2017 Source:BBA Clinical Author(s): Simon P.J. Albracht, Johannes van Pelt With the substrate DNP-α-GalNAc (2,4-dinitrophenyl-N-acetyl-α-d-galactosaminide) three α-N-acetylgalactosaminidase-like activities could be distinguished in serum, in addition to the classical lysosomal enzyme (Naga, EC 3.2.1.49, pH optimum at 4). Two activities had optima in the pH5 to 6 region and one peaked around pH8. Like the Naga activity at pH4, the activity at pH8 was detectable under standard assay conditions. However, the two activities in the pH5 to 6 range were not readily apparent i...
Source: BBA Clinical - September 22, 2017 Category: Biochemistry Source Type: research
Antioxidative activity of high-density lipoprotein (HDL): Mechanistic insights into potential clinical benefit
Publication date: December 2017 Source:BBA Clinical, Volume 8 Author(s): Fernando Brites, Maximiliano Martin, Isabelle Guillas, Anatol Kontush Uptake of low-density lipoprotein (LDL) particles by macrophages represents a key step in the development of atherosclerotic plaques, leading to the foam cell formation. Chemical modification of LDL is however necessary to induce this process. Proatherogenic LDL modifications include aggregation, enzymatic digestion and oxidation. LDL oxidation by one-electron (free radicals) and two-electron oxidants dramatically increases LDL affinity to macrophage scavenger receptors, leadi...
Source: BBA Clinical - September 10, 2017 Category: Biochemistry Source Type: research
Iron retention in iron-fortified rice and use of iron-fortified rice to treat women with iron deficiency: A pilot study
Conclusions Iron-fortified rice increased hemoglobin and hematocrit in women with iron-deficient anemia. Iron deficiency and anemia are widespread in Southeast Asia and Africa and undermine development in these regions. (Source: BBA Clinical)
Source: BBA Clinical - September 9, 2017 Category: Biochemistry Source Type: research
Signatures derived from increase in SHARPIN gene copy number are associated with poor prognosis in patients with breast cancer
We report three signatures produced from SHARPIN gene copy number increase (GCN-Increase) and their effects on patients with breast cancer (BC). In the Metabric dataset (n=2059, cBioPortal), SHARPIN GCN-Increase occurs preferentially or mutual exclusively with mutations in TP53, PIK3CA, and CDH1. These genomic alterations constitute a signature (SigMut) that significantly correlates with reductions in overall survival (OS) in BC patients (n=1980; p=1.081e-6). Additionally, SHARPIN GCN-Increase is associated with 4,220 differentially expressed genes (DEGs). These DEGs are enriched in activation of the pathways regulating ce...
Source: BBA Clinical - August 25, 2017 Category: Biochemistry Source Type: research
Spontaneous miscarriage in first trimester pregnancy is associated with altered urinary metabolite profile.
This study also highlighted the potential of urine metabolites as a non-invasive screening tool for the risk stratification of women presenting with threatened miscarriage. (Source: BBA Clinical)
Source: BBA Clinical - August 19, 2017 Category: Biochemistry Source Type: research
Antioxidant capacity of high-density lipoprotein (HDL): Mechanistic insights into potential clinical benefit
Publication date: Available online 19 August 2017 Source:BBA Clinical Author(s): Fernando Brites, Maximiliano Martin, Isabelle Guillas-Baudouin, Anatol Kontush Uptake of low-density lipoprotein (LDL) particles by macrophages represents a key step in the development of atherosclerotic plaques, leading to the foam cell formation. Chemical modification of LDL is however necessary to induce this process. Proatherogenic LDL modifications include aggregation, enzymatic digestion and oxidation. LDL oxidation by one-electron (free radicals) and two-electron oxidants dramatically increases LDL affinity to macrophage scavenger...
Source: BBA Clinical - August 19, 2017 Category: Biochemistry Source Type: research
Treatment of mild to moderate carpal tunnel syndrome in patients with diabetic neuropathy using low level laser therapy versus ultrasound controlled comparative study
Discussion The results of our study showed that there were no statistical significance differences (P >0.05) were observed between the two groups. It was concluded that both low level laser (20 mw power, 904nm Wavelength) and ultrasound (1.0 w/cm2 power, 1MHz frequency) are effective in the treatment of mild and moderate CTS patients. (Source: BBA Clinical)
Source: BBA Clinical - July 20, 2017 Category: Biochemistry Source Type: research
Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium
Publication date: Available online 3 July 2017 Source:BBA Clinical Author(s): Polina A. Vishnyakova, Maria A. Volodina, Nadezhda V. Tarasova, Maria V. Marey, Natalya E. Kan, Zulfiya S. Khodzhaeva, Mikhail Yu. Vysokikh, Gennady T. Sukhikh Preeclampsia is a pregnancy complication which causes significant maternal and fetal morbidity and mortality worldwide. Although intensive research has been performed in the last 40 years, the pathology of preeclampsia is still poorly understood. The present work is a comparative study of the myometrium of women with normal pregnancy, and those with late- and early-onset preeclam...
Source: BBA Clinical - July 3, 2017 Category: Biochemistry Source Type: research
Abnormal Amyloid β42 expression and increased oxidative stress in plasma of CKD patients with cognitive dysfunction: A small scale case control study comparison with Alzheimer's disease
Conclusion Results suggest that elevated plasma oxidative stress and Aβ were seen in CKD patients with cognitive dysfunction may be attributed to pathological changes within the brain. Graphical abstract (Source: BBA Clinical)
Source: BBA Clinical - June 23, 2017 Category: Biochemistry Source Type: research
Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients
Conclusions A combination of new and standard biomarkers consisting of CLU, ANG, C1Q, albumin, ß2M, paraprotein and K/L ratio may have potential as a novel panel of biomarkers to predict MM response to treatment containing bortezomib. General Significance Use of this biomarker panel could facilitate a more personalized therapy approach and to minimize unnecessary side effects from ineffective drugs. (Source: BBA Clinical)
Source: BBA Clinical - June 8, 2017 Category: Biochemistry Source Type: research
Accumulated substancies and calorific capacity in adipose tissue: Physical and chemical clinical trial
Conclusions Human body lipids serve as a harbor for various organic substances, they may absorb different metabolic products, and they have different calorific capacity depending on their location and forms. Atherosclerotic plaque contains the most of organic and inorganic elements, and brings the highest energy potential. (Source: BBA Clinical)
Source: BBA Clinical - May 31, 2017 Category: Biochemistry Source Type: research
Body mass index is associated with region-dependent metabolic reprogramming of adipose tissue
Publication date: Available online 11 May 2017 Source:BBA Clinical Author(s): Marco G. Alves, Ângela Moreira, Marta Guimarães, Mário Nora, Mario Sousa, Pedro F. Oliveira, Mariana P. Monteiro Adipose tissue (AT) is involved in dysmetabolism pathogenesis. Regional fat distribution and functioning may contribute to obesity-related metabolic disorders and adverse health outcomes. Specific fat depots are suggested to possess unique biological properties, but specific metabolic profiles of subcutaneous (SAT) and visceral adipose tissue (VAT) remain unknown. We aimed to characterize VAT and SAT glucose metabolism, and...
Source: BBA Clinical - May 13, 2017 Category: Biochemistry Source Type: research
Rapid Diagnosis and Intraoperative Margin Assessment of Human Lung Cancer with Fluorescence Lifetime Imaging Microscopy
Publication date: Available online 27 April 2017 Source:BBA Clinical Author(s): Mengyan Wang, Feng Tang, Xiaobo Pan, Longfang Yao, Xinyi Wang, Yueyue Jing, Jiong Ma, Guifang Wang, Lan Mi A method of rapidly differentiating lung tumor from healthy tissue is extraordinarily needed for both the diagnosis and the intraoperative margin assessment. We assessed the ability of fluorescence lifetime imaging microscopy (FLIM) for differentiating human lung cancer and normal tissues with the autofluorescence, and also elucidated the mechanism in tissue studies and cell studies. A 15-patient testing group was used to compar...
Source: BBA Clinical - April 27, 2017 Category: Biochemistry Source Type: research
