VSTM2L is a promising therapeutic target and a prognostic soluble-biomarker in cholangiocarcinoma
BMB Rep. 2024 Apr 23:6090. Online ahead of print.ABSTRACTThe aim of the present study is to provide a rational background for silencing the V-set and transmembrane domain containing 2 like (VSTM2L) in consort with recognising soluble VSTM2L against cholangiocarcinoma. A therapeutic target against cholangiocarcinoma was selected using iterative patient partitioning (IPP) calculation, and it was verified by in vitro and in silico analyses. VSTM2L was selected as a potential therapeutic target against cholangiocarcinoma. Silencing the VSTM2L expression significantly attenuated the viability and survival of cholangiocarcinoma ...
Source: BMB Reports - April 22, 2024 Category: Biochemistry Authors: Jungwhoi Lee Woogwang Sim Jungsul Lee Jae-Hoon Kim Source Type: research
ASCL1-mediated direct reprogramming: converting ventral midbrain astrocytes into dopaminergic neurons for Parkinson's disease therapy
In this study, we aimed to develop a new treatment for PD that induces direct reprogramming of dopaminergic neurons (iDAN). Achaete-scute family bHLH transcription factor 1 (ASCL1) is a primary factor that initiates and regulates central nervous system development and induces neurogenesis. In addition, it interacts with BRN2 and MYT1L, which are crucial transcription factors for the direct conversion of fibroblasts into neurons. Overexpression of ASCL1 along with the transcription factors NURR1 and LMX1A can directly reprogram iDANs. Using a retrovirus, GFP-tagged ASCL1 was overexpressed in astrocytes. One week of culture ...
Source: BMB Reports - April 22, 2024 Category: Biochemistry Authors: Sang Hui Yong Sang-Mi Kim Gyeong Woon Kong Seung Hwan Ko Eun-Hye Lee Yohan Oh Sang Hui Yong Source Type: research
Gut microbiota-generated metabolites: missing puzzles to hosts' health, diseases, and aging
BMB Rep. 2024 Apr 17:6194. Online ahead of print.ABSTRACTThe gut microbiota, an intricate community of bacteria residing in the gastrointestinal system, assumes a pivotal role in various physiological processes. Beyond its function in food breakdown and nutrient absorption, gut microbiota exerts a profound influence on immune and metabolic modulation by producing diverse gut microbiota-generated metabolites (GMGMs). These small molecules hold potential to impact host health via multiple pathways, which exhibit remarkable diversity, and have gained increasing attention in recent studies. Here, we elucidate the intricate imp...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Yan Zhang Shibo Wei Hang Zhang Yunju Jo Jong-Sun Kang Ki-Tae Ha Jongkil Joo Hyun Joo Lee Dongryeol Ryu Source Type: research
3D epigenomics and 3D epigenopathies
BMB Rep. 2024 Apr 17:6163. Online ahead of print.ABSTRACTMammalian genomes are intricately compacted to form sophisticated 3-dimensional structures within the tiny nucleus, so called 3D genome folding. Despite their shapes reminiscent of an entangled yarn, the rapid development of molecular and next-generation sequencing technologies (NGS) has revealed that mammalian genomes are highly organized in a hierarchical order that delicately affects transcription activities. An increasing amount of evidence suggests that 3D genome folding is implicated in diseases, giving us a clue on how to identify novel therapeutic approaches....
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Kyung-Hwan Lee Jungyu Kim Ji Hun Kim Source Type: research
Cisd2 deficiency impairs neutrophil function by regulating calcium homeostasis via Calnexin and SERCA
BMB Rep. 2024 Apr 17:6179. Online ahead of print.ABSTRACTIn the context of aging, the susceptibility to infectious diseases increases, leading to heightened morbidity and mortality. This phenomenon, termed immunosenescence, is characterized by dysregulation in the aging immune system, including abnormal alterations in lymphocyte composition, elevated basal inflammation, and the accumulation of senescent T cells. Such changes contribute to increased autoimmune diseases, enhanced infection severity, and reduced responsiveness to vaccines. Utilizing aging animal models becomes imperative for a comprehensive understanding of i...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Un Yung Choi Youn Jung Choi Shin-Ae Lee Ji-Seung Yoo Source Type: research
Emerging paradigms in cancer cell plasticity
BMB Rep. 2024 Apr 17:6190. Online ahead of print.ABSTRACTCancer cells metastasize to distant organs by altering their characteristics within the tumor microenvironment (TME) to effectively overcome challenges during the multistep tumorigenesis. Plasticity endows cancer cell with the capacity to shift between different states to invade, disseminate, and seed metastasis. The epithelial-to-mesenchymal transition (EMT) is a cellular program that abrogates cell-cell adhesions by EMT transcription factors (TF) and acquires mesenchymal features during cancer progression. On the other hand, adherent-to-suspension transition (AST) ...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Hyunbin D Huh Hyun Woo Park Source Type: research
Prmt7 is required for the osteogenic differentiation of mesenchymal stem cells via modulation of BMP signaling
BMB Rep. 2024 Apr 17:6095. Online ahead of print.ABSTRACTArginine methylation, which is catalyzed by protein arginine methyltransferases (Prmts), is known to play a key role in various biological processes. However, the function of Prmts in osteogenic differentiation of mesenchymal stem cells (MSCs) has not been clearly understood. In the current study, we attempted to elucidate a positive role of Prmt7 in osteogenic differentiation. Prmt7-depleted C3H/10T1/2 cells or bone marrow mesenchymal stem cells (BMSCs) showed the attenuated expression of osteogenic specific genes and Alizarin red staining compared to the wild-type ...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Tuan Anh Vuong Yan Zhang June Kim Young-Eun Leem Jong-Sun Kang Source Type: research
Gut microbiota-generated metabolites: missing puzzles to hosts' health, diseases, and aging
BMB Rep. 2024 Apr 17:6194. Online ahead of print.ABSTRACTThe gut microbiota, an intricate community of bacteria residing in the gastrointestinal system, assumes a pivotal role in various physiological processes. Beyond its function in food breakdown and nutrient absorption, gut microbiota exerts a profound influence on immune and metabolic modulation by producing diverse gut microbiota-generated metabolites (GMGMs). These small molecules hold potential to impact host health via multiple pathways, which exhibit remarkable diversity, and have gained increasing attention in recent studies. Here, we elucidate the intricate imp...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Yan Zhang Shibo Wei Hang Zhang Yunju Jo Jong-Sun Kang Ki-Tae Ha Jongkil Joo Hyun Joo Lee Dongryeol Ryu Source Type: research
3D epigenomics and 3D epigenopathies
BMB Rep. 2024 Apr 17:6163. Online ahead of print.ABSTRACTMammalian genomes are intricately compacted to form sophisticated 3-dimensional structures within the tiny nucleus, so called 3D genome folding. Despite their shapes reminiscent of an entangled yarn, the rapid development of molecular and next-generation sequencing technologies (NGS) has revealed that mammalian genomes are highly organized in a hierarchical order that delicately affects transcription activities. An increasing amount of evidence suggests that 3D genome folding is implicated in diseases, giving us a clue on how to identify novel therapeutic approaches....
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Kyung-Hwan Lee Jungyu Kim Ji Hun Kim Source Type: research
Cisd2 deficiency impairs neutrophil function by regulating calcium homeostasis via Calnexin and SERCA
BMB Rep. 2024 Apr 17:6179. Online ahead of print.ABSTRACTIn the context of aging, the susceptibility to infectious diseases increases, leading to heightened morbidity and mortality. This phenomenon, termed immunosenescence, is characterized by dysregulation in the aging immune system, including abnormal alterations in lymphocyte composition, elevated basal inflammation, and the accumulation of senescent T cells. Such changes contribute to increased autoimmune diseases, enhanced infection severity, and reduced responsiveness to vaccines. Utilizing aging animal models becomes imperative for a comprehensive understanding of i...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Un Yung Choi Youn Jung Choi Shin-Ae Lee Ji-Seung Yoo Source Type: research
Emerging paradigms in cancer cell plasticity
BMB Rep. 2024 Apr 17:6190. Online ahead of print.ABSTRACTCancer cells metastasize to distant organs by altering their characteristics within the tumor microenvironment (TME) to effectively overcome challenges during the multistep tumorigenesis. Plasticity endows cancer cell with the capacity to shift between different states to invade, disseminate, and seed metastasis. The epithelial-to-mesenchymal transition (EMT) is a cellular program that abrogates cell-cell adhesions by EMT transcription factors (TF) and acquires mesenchymal features during cancer progression. On the other hand, adherent-to-suspension transition (AST) ...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Hyunbin D Huh Hyun Woo Park Source Type: research
Prmt7 is required for the osteogenic differentiation of mesenchymal stem cells via modulation of BMP signaling
BMB Rep. 2024 Apr 17:6095. Online ahead of print.ABSTRACTArginine methylation, which is catalyzed by protein arginine methyltransferases (Prmts), is known to play a key role in various biological processes. However, the function of Prmts in osteogenic differentiation of mesenchymal stem cells (MSCs) has not been clearly understood. In the current study, we attempted to elucidate a positive role of Prmt7 in osteogenic differentiation. Prmt7-depleted C3H/10T1/2 cells or bone marrow mesenchymal stem cells (BMSCs) showed the attenuated expression of osteogenic specific genes and Alizarin red staining compared to the wild-type ...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Tuan Anh Vuong Yan Zhang June Kim Young-Eun Leem Jong-Sun Kang Source Type: research
Gut microbiota-generated metabolites: missing puzzles to hosts' health, diseases, and aging
BMB Rep. 2024 Apr 17:6194. Online ahead of print.ABSTRACTThe gut microbiota, an intricate community of bacteria residing in the gastrointestinal system, assumes a pivotal role in various physiological processes. Beyond its function in food breakdown and nutrient absorption, gut microbiota exerts a profound influence on immune and metabolic modulation by producing diverse gut microbiota-generated metabolites (GMGMs). These small molecules hold potential to impact host health via multiple pathways, which exhibit remarkable diversity, and have gained increasing attention in recent studies. Here, we elucidate the intricate imp...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Yan Zhang Shibo Wei Hang Zhang Yunju Jo Jong-Sun Kang Ki-Tae Ha Jongkil Joo Hyun Joo Lee Dongryeol Ryu Source Type: research
3D epigenomics and 3D epigenopathies
BMB Rep. 2024 Apr 17:6163. Online ahead of print.ABSTRACTMammalian genomes are intricately compacted to form sophisticated 3-dimensional structures within the tiny nucleus, so called 3D genome folding. Despite their shapes reminiscent of an entangled yarn, the rapid development of molecular and next-generation sequencing technologies (NGS) has revealed that mammalian genomes are highly organized in a hierarchical order that delicately affects transcription activities. An increasing amount of evidence suggests that 3D genome folding is implicated in diseases, giving us a clue on how to identify novel therapeutic approaches....
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Kyung-Hwan Lee Jungyu Kim Ji Hun Kim Source Type: research
Cisd2 deficiency impairs neutrophil function by regulating calcium homeostasis via Calnexin and SERCA
BMB Rep. 2024 Apr 17:6179. Online ahead of print.ABSTRACTIn the context of aging, the susceptibility to infectious diseases increases, leading to heightened morbidity and mortality. This phenomenon, termed immunosenescence, is characterized by dysregulation in the aging immune system, including abnormal alterations in lymphocyte composition, elevated basal inflammation, and the accumulation of senescent T cells. Such changes contribute to increased autoimmune diseases, enhanced infection severity, and reduced responsiveness to vaccines. Utilizing aging animal models becomes imperative for a comprehensive understanding of i...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Un Yung Choi Youn Jung Choi Shin-Ae Lee Ji-Seung Yoo Source Type: research
