Extraction and analysis of high-quality chloroplast DNA with reduced nuclear DNA for medicinal plants
Obtaining high-quality chloroplast genome sequences requires chloroplast DNA (cpDNA) samples that meet the sequencing requirements. The quality of extracted cpDNA directly impacts the efficiency and accuracy o... (Source: BMC Biotechnology)
Source: BMC Biotechnology - April 18, 2024 Category: Biotechnology Authors: Yifan Yu, Xinxin Wang, Renjun Qu, Zhen OuYang, Juan Guo, Yujun Zhao and Luqi Huang Tags: Research Source Type: research

Transcriptomic and targeted metabolomic analyses provide insights into the flavonoids biosynthesis in the flowers of Lonicera macranthoides
In this study, combined transcriptomic and targeted metabo... (Source: BMC Biotechnology)
Source: BMC Biotechnology - April 12, 2024 Category: Biotechnology Authors: Ling Ling Lv, Li Yun Li, Long Qian Xiao and Jian Hui Pi Tags: Research Source Type: research

Effects of solid lipid nanocarrier containing methyl urolithin A by coating folate-bound chitosan and evaluation of its anti-cancer activity
Nanotechnology-based drug delivery systems have received much attention over the past decade. In the present study, we synthesized Methyl Urolithin A-loaded solid lipid nanoparticles decorated with the folic a... (Source: BMC Biotechnology)
Source: BMC Biotechnology - April 10, 2024 Category: Biotechnology Authors: Ilham Naeem Abd Ali Al-Fatlawi, Vahid Pouresmaeil, Fatemeh Davoodi-Dehaghani, Aida Pouresmaeil, Ali Akhtari and Masoud Homayouni Tabrizi Tags: Research Source Type: research

Neq2X7: a multi-purpose and open-source fusion DNA polymerase for advanced DNA engineering and diagnostics PCR
Thermostable DNA polymerases, such as Taq isolated from the thermophilic bacterium Thermus aquaticus, enable one-pot exponential DNA amplification known as polymerase chain reaction (PCR). However, properties oth... (Source: BMC Biotechnology)
Source: BMC Biotechnology - April 2, 2024 Category: Biotechnology Authors: Cristina Hern ández-Rollán, Anja K. Ehrmann, Arsenios Vlassis, Vijayalakshmi Kandasamy and Morten H. H. Nørholm Tags: Research Source Type: research

A solution for highly efficient electroporation of primary cytotoxic T lymphocytes
Cytotoxic T lymphocytes (CTLs) are central players in the adaptive immune response. Their functional characterization and clinical research depend on efficient and reliable transfection. Although various metho... (Source: BMC Biotechnology)
Source: BMC Biotechnology - March 26, 2024 Category: Biotechnology Authors: Nadia Alawar, Claudia Schirra, Meltem Hohmann and Ute Becherer Tags: Research Source Type: research

Adsorption of Hg2+/Cr6+ by metal-binding proteins heterologously expressed in Escherichia coli
In this study, the m... (Source: BMC Biotechnology)
Source: BMC Biotechnology - March 23, 2024 Category: Biotechnology Authors: Shuting Hu, Zixiang Wei, Teng Liu, Xinyu Zuo and Xiaoqiang Jia Tags: Research Source Type: research

Derivation of a novel antimicrobial peptide from the Red Sea Brine Pools modified to enhance its anticancer activity against U2OS cells
Cancer associated drug resistance is a major cause for cancer aggravation, particularly as conventional therapies have presented limited efficiency, low specificity, resulting in long term deleterious side eff... (Source: BMC Biotechnology)
Source: BMC Biotechnology - March 15, 2024 Category: Biotechnology Authors: Mona Elradi, Ahmed I. Ahmed, Ahmed M. Saleh, Khaled M. A. Abdel-Raouf, Lina Berika, Yara Daoud and Asma Amleh Tags: Research Source Type: research

Polyphyllin B inhibited STAT3/NCOA4 pathway and restored gut microbiota to ameliorate lung tissue injury in cigarette smoke-induced mice
This study plan to explore the mechanism of Polyphyllin B in lung injury induced by cigarette smoke (CSE) in COPD. (Source: BMC Biotechnology)
Source: BMC Biotechnology - March 8, 2024 Category: Biotechnology Authors: Qing Wang, Zhiyi He, Jinqi Zhu, Mengyun Hu, Liu Yang and Hongzhong Yang Tags: Research Source Type: research

Quantifying carboxymethyl lysine and carboxyethyl lysine in human plasma: clinical insights into aging research using liquid chromatography-tandem mass spectrometry
The objective of this study was to establish a methodology for determining carboxymethyl lysine (CML) and carboxyethyl lysine (CEL) concentrations in human plasma using liquid chromatography-tandem mass spectr... (Source: BMC Biotechnology)
Source: BMC Biotechnology - March 7, 2024 Category: Biotechnology Authors: Daguang Wang, Junshan Wang, Xinghong Liu, Kehe Du, Hongjun Liu, Xiaofeng Yang, Tianyi Liu, Qian Liu, Meng Wang and Jian Guo Tags: Research Source Type: research

Iron/Copper/Phosphate nanocomposite as antimicrobial, antisnail, and wheat growth-promoting agent
One of the current challenges is to secure wheat crop production to meet the increasing global food demand and to face the increase in its purchasing power. Therefore, the current study aimed to exploit a new ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - March 5, 2024 Category: Biotechnology Authors: Nashwa H. Abdullah, Nouran A. Elbialy, Mohamed Abdelnaser Amer, Mostafa Kh. Gabr, Amira Salah El-Din Youssef, Mohamed H. Sharaf, M. E. Shehata, Mohamed H. Kalaba and Elham R. S. Soliman Tags: Research Source Type: research

Staphopain mediated virulence and antibiotic resistance alteration in co-infection of Staphylococcus aureus and Pseudomonas aeruginosa: an animal model
Polymicrobial communities lead to worsen the wound infections, due to mixed biofilms, increased antibiotic resistance, and altered virulence production. Promising approaches, including enzymes, may overcome th... (Source: BMC Biotechnology)
Source: BMC Biotechnology - March 4, 2024 Category: Biotechnology Authors: Sanaz Dehbashi, Hamed Tahmasebi, Mohammad Yousef Alikhani, Mohammad-Ali Shahbazi and Mohammad Reza Arabestani Tags: Research Source Type: research

Strain-specific features of Pleurotus ostreatus growth in vitro and some of its biological activities
The production of Pleurotus ostreatus mycelium as a promising object for use in food and other industries is hampered by a lack of information about the strain-specificity of this fungus mycelium growth and its a... (Source: BMC Biotechnology)
Source: BMC Biotechnology - February 8, 2024 Category: Biotechnology Authors: Tetiana Krupodorova, Victor Barshteyn, Victoria Tsygankova, Mustafa Sevindik and Yaroslav Blume Tags: Research Source Type: research

Antibacterial, antibiofilm, and anticancer activity of silver-nanoparticles synthesized from the cell-filtrate of Streptomyces enissocaesilis
In this study, microbial nanotechnology is employed to synthesize ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - February 6, 2024 Category: Biotechnology Authors: Mohamed T. Shaaban, Briksam S. Mohamed, Muhammad Zayed and Sabha M. El-Sabbagh Tags: Research Source Type: research

Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitata
The Mediterranean fruit fly, Ceratitis capitata, is a significant agricultural pest managed through area-wide integrated pest management (AW-IPM) including a sterile insect technique (SIT) component. Male-only re... (Source: BMC Biotechnology)
Source: BMC Biotechnology - February 1, 2024 Category: Biotechnology Authors: Germano Sollazzo, Katerina Nikolouli, Georgia Gouvi, Roswitha A. Aumann, Marc F. Schetelig and Kostas Bourtzis Tags: Research Source Type: research

Characterization, modeling, and anticancer activity of L.arginase production from marine Bacillus licheniformis OF2
L-arginase, is a powerful anticancer that hydrolyzes L-arginine to L-ornithine and urea. This enzyme is widely distributed and expressed in organisms like plants, fungi, however very scarce from bacteria. Our ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 25, 2024 Category: Biotechnology Authors: Manal S. Selim, Marwa M. Mounier, Sayeda A. Abdelhamid, Ahmed Abdelghani Hamed, Mostafa M. Abo Elsoud and Sahar S. Mohamed Tags: Research Source Type: research