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Non-antibiotic selection systems for soybean somatic embryos: the lysine analog aminoethyl-cysteine as a selection agentEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Genetically engineered soybeans expressing a lysine insensitive DHPS gene can be selected with the non-antibiotic selection agent AEC. We also report here the inhibitory effects of glufosinate, (isopropylamine-glyphosate) (Roundup(R)), AEC and the ALS inhibitors Exceed(R) and Synchrony(R) against different tissues of soybean. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - November 18, 2009 Category: Biotechnology Authors: Suryadevara RaoLewamy MamadouMatt McConnellRaghuveer PolisettyPrachuab KwanyuenDavid Hildebrand Source Type: journals

Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modulesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We conclude that affinity maturation of CBM selected from molecular libraries based on the CBM4-2 scaffold is possible and has the potential to generate new analytical tools for detection of plant carbohydrates. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - October 31, 2009 Category: Biotechnology Authors: Laura von SchantzFredrika GullfotSebastian ScheerLada FilonovaLavinia Cicortas GunnarssonJames FlintGeoffrey DanielEva Nordberg-KarlssonHarry BrumerMats Ohlin Source Type: journals

Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard) x Brassica napus (oilseed rape) hybrid populationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The effects of genetic load of crop and in some situations, weed alleles might be beneficial under certain environmental conditions. However, when genetic load was directly incorporated into transgenic events, e.g., using a TM construct, the number of transgenic hybrids and persistence in weedy genomic backgrounds was significantly decreased. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - October 31, 2009 Category: Biotechnology Authors: Christy RoseReginald MillwoodHong Seok MoonMurali RaoMatthew HalfillPaul RaymerSuzanne WarwickHani Al-AhmadJonathan GresselC Neal Stewart Source Type: journals

Novel immortalized human fetal liver cell line, cBAL111, has the potential to differentiate into functional hepatocytesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This novel liver cell line has the potential to differentiate into mature hepatocytes to be used for in vitro hepatocyte applications. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - October 20, 2009 Category: Biotechnology Authors: Tanja DeurholtNiek van TilAniska ChhattaLysbeth ten BloemendaalRuth SchwartlanderCatherine PayneJohn PlevrisIgor SauerRobert ChamuleauRonald Oude ElferinkJurgen SeppenRuurdtje Hoekstra Source Type: journals

Characterization of unknown genetic modifications using high throughput sequencing and computational subtractionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: We believe that computational subtraction represents a powerful new strategy for determining if an organism has been genetically modified as well as to define the nature of the modification. Fewer assumptions have to be made compared to methods currently in use and this is an advantage particularly when working with unknown GMOs. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - October 7, 2009 Category: Biotechnology Authors: Torstein TengsHaibo ZhangArne Holst-JensenJon BohlinMelinka ButenkoAnja Brathen KristoffersenHilde-Gunn Opsahl SortebergKnut Berdal Source Type: journals

Refinement of lentiviral vector for improved RNA processing and reduced rates of self inactivation repairEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: RNA processing can be improved by mutation of the major and minor HIV-1 splice donor sites in the vector. In addition the rate of vector mobilisation and repair of SIN vectors can be successfully reduced by careful vector design that reduces homology between the 5' and 3' long terminal repeats (LTRs) to a minimum. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - October 6, 2009 Category: Biotechnology Authors: Rachel KoldejDonald Anson Source Type: journals

Reduction of liver macrophage transduction by pseudotyping lentiviral vectors with a fusion envelope from Autographa californica GP64 and Sendai virus F2 domain.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We demonstrate reduced macrophage transduction in vitro and in vivo with GP64/Sendai chimeric envelope proteins. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - October 6, 2009 Category: Biotechnology Authors: David MarkusicNiek van TilJohan HiralallRonald Oude ElferinkJurgen Seppen Source Type: journals

Thermochemotherapy effect of nanosized As2O3/Fe3O4 complex on experimental mouse tumors and its influence on the expression of CD44v6, VEGF-C and MMP-9Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Background: Both thermotherapy and arsenic have been shown to be active against a broad spectrum of cancers. To reduce the limitations of conventional thermotherapy, improve therapeutic anticancer activity, reduce the toxicity of arsenic on normal tissue, and increase tissue-specific delivery, we prepared a nanosized As2O3/Fe3O4 complex (Fe3O4 magnetic nanoparticles encapsulated in As2O3). We assessed the thermodynamic characteristics of this complex and validated the hyperthermia effect, when combined with magnetic fluid hyperthermia (MFH), on xenograft HeLa cells (human cervical cancer cell line) in nude mice. We also me...
Source: BMC Biotechnology - Latest articles - October 4, 2009 Category: Biotechnology Authors: Yiqun DuDongsheng ZhangHui LiuRensheng Lai Source Type: journals

Integrating human stem cell expansion and neuronal differentiation in bioreactorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The culture systems developed herein are robust and represent one-step-forward towards the development of integrated bioprocesses, bridging stem cell expansion and differentiation in fully controlled bioreactors. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - September 21, 2009 Category: Biotechnology Authors: Margarida SerraCatarina BritoEunice CostaMarcos SousaPaula Alves Source Type: journals

A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This new Tol2/MACS system leads to stable expression in a culture of primary chicken erythroid cells highly enriched in cells expressing the transgene of interest. This system could be used in a wide variety of vertebrate species. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - September 17, 2009 Category: Biotechnology Authors: Camila Mejia-PousJose VinuelasClaudine FaureJoanna KoszelaKoichi KawakamiYoshiko TakahashiOlivier Gandrillon Source Type: journals

Antibody expressing pea seeds as fodder for prevention of gastrointestinal parasitic infections in chickensEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The results suggest that our strategy offers a general approach to control parasitic infections in production animals using cost-effective antibody expression in crop seeds affordable for the animal health market. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - September 10, 2009 Category: Biotechnology Authors: Jana ZimmermannIsolde SaalbachDoreen JahnMartin GiersbergSigrun HaehnelJulia WedelJeanette MacekKaren ZoufalGerhard GlunderDieter FalkenburgSergej Kiprijanov Source Type: journals

Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivoEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: A rational purification protocol for TAT-Cre has been developed by choosing the options that minimize the enzyme aggregation. Our data suggest that AI measurement should support the optimization of any protocol aiming at the recovery of monodispersed protein. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - September 10, 2009 Category: Biotechnology Authors: Paola CapassoMarisa AliprandiGiuseppe OssolengoFrank EdenhoferArio de Marco Source Type: journals

New stably transfected bioluminescent cells expressing FLAG epitope-tagged estrogen receptors to study their chromatin recruitmentEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: In HELN derived cell lines, no fundamental differences between kinetics were observed during 8 hours for FLAG-ERalpha and FLAG-ERbeta, as well as for polymerase II recruitment. However, the relative importance of recruitment between 1 hr and 4 hr was found to be different in HeLa cell line expressing exogenous tagged ERalpha and in MCF-7 cell line expressing endogenous ER. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - September 8, 2009 Category: Biotechnology Authors: Eric BadiaAurelie EscandePatrick BalaguerRaphael MetivierVincent Cavailles Source Type: journals

A comparative study of recombinant and native frutalin binding to human prostate tissuesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Native and recombinant frutalin yielded different binding responses in the prostate tissues due to their differences in carbohydrate-binding affinities. Also, this study shows that both lectins may be used as histochemical biomarkers for the prostate cancer. Moreover, the successful use of a recombinant lectin in immunohistochemical studies of prostate cancer was for the first time demonstrated, highlighting the advantages of using recombinant systems in the preparation of pure lectin samples for diagnostic purpose. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - September 8, 2009 Category: Biotechnology Authors: Carla OliveiraJose TeixeiraFernando SchmittLucilia Domingues Source Type: journals

Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The ability to evaluate priming (and mispriming) rates and to predict their impacts provided a precise and quantitative description of assay performance. Priming probabilities were also found to be a good measure of analytical specificity. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - August 27, 2009 Category: Biotechnology Authors: Brian BoyleNancy DallaireJohn MacKay Source Type: journals

Transduction of the MPG-tagged fusion protein into mammalian cells and oocytes depends on amiloride-sensitive endocytic pathwayEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The results show that the transduction of MPG fusion protein utilizes endocytic pathway(s) which is amiloride-sensitive and partially dynamin-dependent. Collectively, the MPG fusion protein could be further developed as a novel tool of "protein therapeutics", with potentials to be used in various cell systems including mammalian oocytes. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - August 25, 2009 Category: Biotechnology Authors: So-Jung KwonKyuyong HanSuhyun JungJong-Eun LeeSeongsoon ParkYong-Pil CheonHyunjung Lim Source Type: journals

Directed evolution of a filamentous fungus for thermotoleranceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: We report the experimental evolution of a filamentous fungus via the novel application of a powerful new continuous culture device. This is the first example of using continuous culture to select for complex phenotypes such as thermotolerance. Temperature adapted variants of the insect-pathogenic, filamentous fungus M. anisopliae were isolated and demonstrated to show vigorous growth at a temperature that is inhibitory for the parent strain. Insect virulence assays confirmed that pathogenicity can be retained during the selection process. In principle, this technology can be used to adapt filamentous fungi to v...
Source: BMC Biotechnology - Latest articles - August 25, 2009 Category: Biotechnology Authors: Eudes de CrecyStefan JaronskiBenjamin LyonsThomas LyonsNemat Keyhani Source Type: journals

High quality protein microarray using in situ protein purificationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: An optimized platform for in situ protein purification on microarray slides using His-tagged recombinant proteins is a desirable tool for the screening of novel protein functions and protein-protein interactions. In the context of immunoproteomics, such protein microarrays are complimentary to approaches using non-recombinant methods to discover and characterize bacterial antigens. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - August 22, 2009 Category: Biotechnology Authors: Keehwan KwonCarissa GroseRembert PieperGagan PandyaRobert FleischmannScott Peterson Source Type: journals

Archaeosomes made of Halorubrum tebenquichensetotal polar lipids: a new source of adjuvancyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: We herein report the finding of new H. tebenquichense non alkaliphilic strains in Argentinean Patagonia together with the adjuvant properties of ARC after sc administration in mice. Our results indicate that archaeosomes prepared with TPL from these two strains could be successfully used as vaccine delivery vehicles. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - August 12, 2009 Category: Biotechnology Authors: Raul GonzalezLeticia HigaRomina CutrullisMarcos BilenIrma MorelliDiana RoncagliaRicardo CorralMaria Jose MorillaPatricia PetrayEder Romero Source Type: journals

Archaeosomes made of Halorubrum tebenquichense total polar lipids: A new source of adjuvancyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We herein report the finding of new H. tebenquichense non alkaliphilic strains in Argentinean Patagonia together with the adjuvant properties of ARC after sc administration in mice. Our results indicate that archaeosomes prepared with TPL from these two strains could be successfully used as vaccine delivery vehicles. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - August 12, 2009 Category: Biotechnology Authors: Raul GonzalezLeticia HigaRomina CutrullisMarcos BilenIrma MorelliDiana RoncagliaRicardo CorralMaria Jose MorillaPatricia PetrayEder Romero Source Type: journals

Efficient generation of long-distance conditional alleles using recombineering and a dual selection strategy in replicate platesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The strategy of ES cell cultures in replicate plates proved to be very efficient in identifying ES cells that had undergone the correct recombination event. This approach facilitates the generation of conditional knock-out mice when large parts of the genome are intended to be flanked by loxP sites. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - July 27, 2009 Category: Biotechnology Authors: David VoehringerDavina WuHong-Erh LiangRichard Locksley Source Type: journals

Evaluation of cross-linked aggregates from purified Bacillus subtilis levansucrase mutants for transfructosylation reactionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This is the first report of cross-linked transglycosidases aggregates. CLEAs prepared from purified LS and mutants have the highest specific activity for immobilized fructosyltransferases (FTFs) reported in the literature. CLEAs from R360K and Y429N LS mutants were particularly suitable for fructosyl-xyloside synthesis as the absence of levan synthesis decreases diffusion limitation and increases operational stability. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - July 26, 2009 Category: Biotechnology Authors: Maria Elena Ortiz-SotoEnrique Rudino-PineraMaria Elena Rodriguez-AlegriaAgustin Lopez Munguia Source Type: journals

Gamma-phage lysin PlyG sequence-based synthetic peptides coupled with Qdot-nanocrystals are useful for developing detection methods for Bacillus anthracis by using its surrogates, B. anthracis-Sterne and B. cereus-4342Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Overall, these studies illustrate that the short 10-amino acid sequence 'LKMTADFILQ' in fact is a stand-alone bacterial cell wall-binding motif of PlyG. In principle, synthetic peptides PlyG-P1, PlyG-P3 and PlyG-P5, especially PlyG-P3 coupled with Qdot-nanocrystals are useful as high-sensitivity bio-probes in developing detection technologies for B. anthracis. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - July 21, 2009 Category: Biotechnology Authors: Shilpakala SainathraoKetha Krishna MohanChintamani Atreya Source Type: journals

Efficient, chemoselective synthesis of immunomicelles using single-domain antibodies with a C-terminal thioesterEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: A novel procedure was developed to obtain soluble, well-folded single-domain antibodies with reactive C-terminal thioesters in good yields. These proteins are promising building blocks for the chemoselective functionalization via NCL of a broad range of nanoparticle scaffolds, including micelles, liposomes and dendrimers. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - July 19, 2009 Category: Biotechnology Authors: Sanne ReulenIngrid van BaalJos RaatsMaarten Merkx Source Type: journals

A novel Poly(epsilon-caprolactone)-Pluronic-Poly(epsilon-caprolactone) grafted Polyethyleneimine(PCFC-g-PEI), Part 1, synthesis, cytotoxicity, and in vitro transfection studyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The cytotoxicity and in vitro transfection study indicated that PCFC-g-PEI copolymer prepared in this paper was a novel gene delivery system with lower cytotoxicity and considerable transfection efficiency compared with commercial PEI (25kD). (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - July 16, 2009 Category: Biotechnology Authors: Shuai ShiQingFa GuoBing KanShaoZhi FuXiuHong WangChangYang GongHongXin DengFeng LuoXia ZhaoYuQuan WeiZhiYong Qian Source Type: journals

Stable nuclear transformation of Gonium pectoraleEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Flanking sequences that include promoters from C. reinhardtii and from V. carteri work in G. pectorale and allow the functional expression of heterologous genes, such as the selectable marker gene aphVIII of S. rimosus or the co-transformed, codon-optimized G. princeps luciferase gene, which turned out to be a suitable reporter gene in Gonium. The availability of a method for transformation of Gonium makes genetic engineering of this species possible and allows for detailed studies in molecular evolution using the unicellular Chlamydomonas, the 16-celled Gonium, and the multicellular Volvox. (Source: BMC Biotec...
Source: BMC Biotechnology - Latest articles - July 9, 2009 Category: Biotechnology Authors: Kai LercheArmin Hallmann Source Type: journals

Production of N-acetyl-D-neuraminic Acid Using Two Sequential Enzymes Overexpressed as Double-Tagged Fusion ProteinsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Double-tagged gene fusions were proposed for over-expressing two sequential enzyme proteins, which could be easily immobilzed onto ionic exchangers by direct capture from the crude prtoein extracts via ionic tags. The immobilized, double-tagged proteins were demonstrated to be effective for one-pot enzymatic production of sialic acid. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - July 8, 2009 Category: Biotechnology Authors: Tzu-Hsien WangYing-Yin ChenHsin-Hung PanFeng-Pao WangChung-Hsien ChengWen-Chien Lee Source Type: journals

A further insight into the biosorption mechanism of Pt() by infrared spectrometryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: That knowledge could serve as a guide in the researches for improving the preparation of highly dispersive supported platinum catalyst and for fabricating new advanced platinum nanostructured devices by biotechnological methods. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - July 5, 2009 Category: Biotechnology Authors: Zhongyu LinRu XueYiwen YeJianhong ZhengZhenling Xu Source Type: journals

Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Results of the characterization studies, and the high degree of identity with human germline genes, altogether make this anti-ricin scFv, or an IgG derived from it, a likely candidate for use in humans to minimize effects caused by ricin intoxication. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - June 29, 2009 Category: Biotechnology Authors: Thibaut PelatMichael HustMartha HaleMarie-Paule LefrancStefan DubelPhilippe Thullier Source Type: journals

Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The SPRINP mutagenesis protocol yields mutants reliably and with high fidelity. The use of a single primer in each amplification reaction increases the probability of success of primers relative to previous methods employing a forward and reverse primer pair in the same reaction. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - June 29, 2009 Category: Biotechnology Authors: Oded EdelheitAaron HanukogluIsrael Hanukoglu Source Type: journals

Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastoris and functional characterization of the produced enzymeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The P. pastoris expression system allows a high level expression of xylanases. Xylanase was the main protein species in the culture supernatant, and the functional tests indicated that even the non-purified enzyme shows highly specific xylanase activity that is free of cellulolytic side acitivities. Therefore, P pastoris is a very useful expression system when the goal is highly specific and large scale production of glycosyl hydrolases. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - June 15, 2009 Category: Biotechnology Authors: Jun HeBing YuKeying ZhangXuemei DingDaiwen Chen Source Type: journals

Interfering ribonucleic acids that suppress expression of multiple unrelated genesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Our work demonstrates the possibility of designing multitargeting dsRNA to suppress more than one disease-altering gene. This warrants further investigation as a possible therapeutic approach. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - June 15, 2009 Category: Biotechnology Authors: Toby PassiouraMary GozarAmber GoodchildAndrew KingGreg ArndtMichael PoidingerDonald BirkettLaurent Rivory Source Type: journals

High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterizationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The successful purification of rBmNPV-hPRR indicates that baculovirus production using silkworm larvae and its purification from hemolymph by Sephacryl S-1000 SF column chromatography can provide an economical approach in obtaining the purified BmNPV stocks with high titer for large-scale production of hPRR. Also, it can be utilized for further binding analysis and screening of inhibitors of hPRR. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - June 11, 2009 Category: Biotechnology Authors: Tatsuya KatoSuganthi ManoharShigeyasu TanakaEnoch Park Source Type: journals

Synthesis of sialoglycopolypeptide for potentially blocking Influenza virus infection using a rat alpha2,6-sialyltransferase expressed in BmNPV bacmid-injected silkworm larvaeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The recombinant alpha2,6SiaT from a silkworm expression system is useful for the synthesis of sialylation of asialoglycopeptide and a valuable tool for investigating the molecular mechanisms of biological and physiological events, such as cell-cell recognition and viral infections. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - June 5, 2009 Category: Biotechnology Authors: Makoto OgataMakoto NakajimaTatsuya KatoTakikiyo ObaraHirokazu YagiKoichi KatoTaichi UsuiEnoch Y. Park Source Type: journals

Construction of siRNA/miRNA expression vectors based on a one-step PCR processEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Background: RNA interference (RNAi) has become a powerful means for silencing target gene expression in mammalian cells and is envisioned to be useful in therapeutic approaches to human disease. In recent years, high-throughput, genome-wide screening of siRNA/miRNA libraries has emerged as a desirable approach. Current methods for constructing siRNA/miRNA expression vectors require the synthesis of long oligonucleotides, which is costly and suffers from mutation problems. Results: Here we report an ingenious method to solve traditional problems associated with construction of siRNA/miRNA expression vectors. We synthesized ...
Source: BMC Biotechnology - Latest articles - June 2, 2009 Category: Biotechnology Authors: Jun XuJie ZengGang WanGui HuHong YanLi Ma Source Type: journals

Integrated application of uniform design and least-squares support vector machines to transfection optimizationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The integrated application of uniform design and least-squares support vector machine is a simple technique for obtaining high transfection efficiency. Using this novel method, the number of required experiments would be greatly cut down while higher efficiency would be gained. Least-squares support vector machine may be applicable to many other problems that need to be optimized. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - May 31, 2009 Category: Biotechnology Authors: Jin-Shui PanMei-Zhu HongQi-Feng ZhouJia-Yan CaiHua-Zhen WangLin-Kai LuoDe-Qiang YangJing DongHua-Xiu ShiJian-Lin Ren Source Type: journals

A microarray-based method for the parallel analysis of genotypes and expression profiles of wood-forming tissues in Eucalyptus grandisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: A simple, yet general method was developed for genotyping and expression profiling of wood-forming tissue of E. grandis trees differing in their splitting characteristics and in their lignin contents. Evaluation of gene expression profiles and the binary distribution of cDNA-AFLP fragments on the chip suggest that the prototype chip developed could be useful for transcript profiling and for the identification of Eucalyptus trees with preferred wood quality traits in commercial breeding programmes. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - May 27, 2009 Category: Biotechnology Authors: Eugenia BarrosCarol-Ann van StadenSabine Lezar Source Type: journals

Expanding the multicolor capabilities of basic confocal microscopes by employing red and near-infrared quantum dot conjugatesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Our data demonstrate that nearly any basic confocal microscope can be modified by the simple addition of appropriate emission filters, allowing the detection of red and near-infrared quantum dot conjugates. Additionally, quantum dot- and organic dye-based secondary reagents can be successfully combined in complex intracellular staining experiments. Substantial expansion of the multi-parameter capabilities of basic confocal instruments can be achieved with a financial investment that is minimal in comparison to instrument replacement or upgrade with additional lasers. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - May 22, 2009 Category: Biotechnology Authors: Lara KingeterBrian Schaefer Source Type: journals

Cost-effective HRMA pre-sequence typing of clone libraries; application to phage display selectionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Using HRMA, analysis of up to 384 samples can be done simultaneously and will take approximately 30 minutes. Clustering of clones can be largely automated using the system's software within 2 hours. Applied to the analysis of clones obtained after phage display antibody selection, HRMA facilitated a quick overview of the overall success as well as the identification of identical clones. Our approach can be used to characterize any clone set prior to sequencing, thereby reducing sequencing costs significantly. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - May 22, 2009 Category: Biotechnology Authors: Barry PepersMenno SchutRolf VossenGert-Jan van OmmenJohan den DunnenWilleke van Roon-Mom Source Type: journals

Luciferase activity under direct ligand-dependent control of a muscarinic acetylcholine receptorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Our results demonstrate that in engineered GPCR-enzyme chimeras, intracellular enzyme activity can be directly controlled by a GPCR serving as the extracellular ligand-binding domain. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - May 18, 2009 Category: Biotechnology Authors: Doreen Thor, Diana Le Duc, Rainer Strotmann and Torsten Schoneberg Source Type: journals

RNAi in the cereal weevil Sitophilus spp: Systemic gene knockdown in the bacteriome tissueEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: These results demonstrate that whole larval injection with dsRNA results in a high and systemic decrease of both mRNA and protein in the bacteriome tissue. This, along with the possibility of access to the insect developmental stages, opens up a new research avenue for exploring gene specific functions in the cereal weevils. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - May 15, 2009 Category: Biotechnology Authors: Agnes Vallier, Carole Vincent-Monegat, Anne Laurencon and Abdelaziz Heddi Source Type: journals

Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applicationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We have successfully demonstrated efficient combination of NASBA amplification technology with microarray based hybridization detection. Method is applicative for many different areas of microbial diagnostics including environmental monitoring, bio threat detection, industrial process monitoring and clinical microbiology. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - May 15, 2009 Category: Biotechnology Authors: Ott Scheler, Barry Glynn, Sven Parkel, Priit Palta, Kadri Toome, Lauris Kaplinski, Maido Remm, Majella Maher and Ants Kurg Source Type: journals

The CD-loop of PAI-2 (SERPINB2) is redundant in the targeting, inhibition and clearance of cell surface uPA activityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We demonstrate that the CD-loop is redundant for the purposes of cellular uPA inhibition and cell surface clearance (endocytosis) and is thus suitable for the development of anti-uPA targeted cancer therapeutics. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - May 14, 2009 Category: Biotechnology Authors: Blake J Cochran, Lakshitha P Gunawardhana, Kara L Vine, Jodi A Lee, Sergei Lobov and Marie Ranson Source Type: journals

Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteinsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: FLSSM allows rapid visualization of the high secretors from the transfected pools prior to picking, thus eliminating the tedious task of screening a high number of cell isolates. Because of its rapidity and its simplicity, FLSSM is a versatile method for the screening of high producers for research and industry. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - May 11, 2009 Category: Biotechnology Authors: Antoine W Caron, Claire Nicolas, Bruno Gaillet, Ismaila Ba, Maxime Pinard, Alain Garnier, Bernard Massie and Renald Gilbert Source Type: journals

A suite of recombinant luminescent bacterial strains for the quantification of bioavailable heavy metals and toxicity testingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The constructed battery of 19 luminescent sensor strains exhibits several novel aspects as it contains i) different bacterial hosts with similar metal-responsive elements ii) similar bacteria with metal-responsive elements in different copies in the cell and iii) a lights-off control for every metal sensor strain. No Gram-positive metal sensor expressing full bacterial bioluminescence system was constructed earlier. The combinations of different constructed strains enabled to follow the effect of the bacterial host on their sensitivity and specificity as well as on bioavailability of metals in various samples....
Source: BMC Biotechnology - Latest articles - May 8, 2009 Category: Biotechnology Authors: Angela Ivask, Taisia Rolova and Anne Kahru Source Type: journals

Efficient CPP -mediated Cre protein delivery to developing and adult CNS tissues.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We provide tools for the spatially restricted genetic modification of cells in explant culture. This strategy allows to study lineage, migration, differentiation and death of neural cells. As a proof-of-concept applied to CNS tissue, direct delivery of Cre recombinase enabled the selective elimination of an interneuron subpopulation of the spinal cord, thereby providing a model to study early events of neurodegenerative processes. Thus our work opens new perspectives for both fundamental and applied cell targeting protocols using proteic cargoes which need to retain full bioactivity upon internalisation, as il...
Source: BMC Biotechnology - Latest articles - April 24, 2009 Category: Biotechnology Authors: Yorick Gitton, Lorenzo Tibaldi, Edmond Dupont, Giovanni Levi and Alain Joliot Source Type: journals

Acetylation of PAMAM dendrimers for cellular delivery of siRNAEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Primary amine acetylation of PAMAM dendrimers reduced their cytotoxicity to U87 cells, and promoted the release of siRNA from dendrimer/siRNA complexes. A modest fraction (approximately 20%) of primary amines of PAMAM can be modified while maintaining the siRNA delivery efficiency of unmodified PAMAM, but higher degrees of amine neutralization reduced the gene silencing efficiency of PAMAM/siRNA delivery vectors. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - April 23, 2009 Category: Biotechnology Authors: Carolyn L Waite, Sarah M Sparks, Kathryn E Uhrich and Charles M Roth Source Type: journals

A modified and automated version of the 'Fluorimetric Detection of Alkaline DNA Unwinding' method to quantify formation and repair of DNA strand breaksEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: We have established a robust and convenient method measuring of formation and repair of DNA single-strand breaks in live cells. While the sensitivity of our method is comparable to current assays, throughput is massively increased while operator time is decreased. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - April 23, 2009 Category: Biotechnology Authors: Maria Moreno-Villanueva, Ragen Pfeiffer, Thilo Sindlinger, Alan Leake, Marcus Muller, Thomas BL Kirkwood and Alexander Burkle Source Type: journals

Gene Composer: database software for protein construct design, codon engineering, and gene synthesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We present a complete description of Gene Composer functionality, and an efficient PCR-based synthetic gene assembly procedure with mis-match specific endonuclease error correction in combination with PIPE cloning. In a sister manuscript we present data on how Gene Composer designed genes and protein constructs can result in improved protein production for structural studies. (Source: BMC Biotechnology - Latest articles)
Source: BMC Biotechnology - Latest articles - April 21, 2009 Category: Biotechnology Authors: Don Lorimer, Amy Raymond, John Walchli, Mark Mixon, Adrienne Barrow, Ellen Wallace, Rena Grice, Alex Burgin and Lance Stewart Source Type: journals

Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene ComposerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The results consistently demonstrate that protein yields from codon engineered Gene Composer designs are as good as or better than those achieved from the synonymous native genes. Moreover, structure guided N- and C-terminal deletion constructs designed with the aid of Gene Composer can lead to greater success in gene to structure work as exemplified by the X-ray crystallographic structure determination of FtsZ from Bacillus subtilis. These results validate the Gene Composer algorithms, and suggest that using a combination of synthetic gene and protein construct engineering tools can improve the economics of ge...
Source: BMC Biotechnology - Latest articles - April 21, 2009 Category: Biotechnology Authors: Amy Raymond, Scott Lovell, Don Lorimer, John Walchli, Mark Mixon, Ellen Wallace, Kaitlin Thompkins, Kimberly Archer, Alex Burgin and Lance Stewart Source Type: journals