BMC Microbiology - Latest articles
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Correction: Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic Escherichia coli strains with different uncommon intimin types
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Discussion" (Page 3, Paragraph 1) and in "Methods" ("Typing of intimin genes", Page 8), intimin upsilon (Greek letter- u) appears again with a wrong symbol (n). (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - November 11, 2009 Category: Microbiology Authors: Denise YamamotoRodrigo HernandesMiguel BlancoLilo GreuneM Alexander SchmidtSylvia CarneiroGhizlane DahbiJesus BlancoAzucena MoraJorge BlancoTania Gomes Source Type: journals
Performance of optimized McRAPD in identification of 9 yeast species frequently isolated from patient samples: potential for automation
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Conclusions:
Since McRAPD technique omits gel electrophoresis and can be performed in a rapid, economical and convenient way, we believe that it can find its place in routine identification of medically important yeasts in advanced diagnostic laboratories that are able to adopt this technique. It can also serve as a broad-range high-throughput technique for epidemiological surveillance. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - November 10, 2009 Category: Microbiology Authors: Jitka TrtkovaPetr PavlicekLenka RuskovaPetr HamalDagmar KoukalovaVladislav Raclavsky Source Type: journals
Environmental stresses inhibit splicing in the aquatic fungus Blastocladiella emersonii
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Conclusions:
In conclusion, data indicate that environmental stresses, particularly cadmium treatment, inhibit intron processing in B. emersonii, revealing a new deleterious effect of cellular exposure to this heavy metal. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 29, 2009 Category: Microbiology Authors: Raphaela GeorgRosane StefaniSuely Gomes Source Type: journals
Application and evaluation of the MLVA typing assay for the Brucella abortus strains isolated in Korea
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Conclusions:
The MLVA assay has enough discrimination power in the Brucella species level and can be utilized as a tool for the epidemiological trace-back of the B. abortus isolates. But it is important to consider that Brucella isolates may be capable of undergoing minor changes at some loci in the course of infection or in accordance with the changes of the host. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 29, 2009 Category: Microbiology Authors: Moon HerSung-Il KangDong-Hee ChoYun-Sang ChoIn-Yeong HwangYoung-Ran HeoSuk-Chan JungHan-Sang Yoo Source Type: journals
Influences of naturally occurring agents in combination with fluoride on gene expression and structural organization of Streptococcus mutans in biofilms
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Background:
The association of specific bioactive flavonoids and terpenoids with fluoride can modulate the development of cariogenic biofilms by simultaneously affecting the synthesis of exopolysaccharides (EPS) and acid production by Streptococcus mutans, which enhanced the cariostatic effectiveness of fluoride in vivo. In the present study, we further investigated whether the biological actions of combinations of myricetin (flavonoid), tt-farnesol (terpenoid) and fluoride can influence the expression of specific genes of S. mutans within biofilms and their structural organization using real-time PCR and confocal fluoresc...
Source: BMC Microbiology - Latest articles - October 28, 2009 Category: Microbiology Authors: Jae-Gyu JeonMarlise KleinJin XiaoStacy GregoirePedro RosalenHyun Koo Source Type: journals
Staphylococcus aureus biofilm formation at the physiologic glucose concentration depends on the S. aureus lineage
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Conclusions:
The adherence to polystyrene surfaces under physiologic glucose concentration (0.1%) was dependent on the clonal lineage. Strains associated with MLST CC8 were markedly more often classified as strong biofilm former at glucose concentrations of 0%, 0.1% and 0.25%.The present study reveals that the MLST CC8 associated genetic background was a predisposing factor for strong biofilm formation in vitro, under all tested glucose concentrations. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 28, 2009 Category: Microbiology Authors: Sander CroesRuud DeurenbergMarie-Louise BoumansPatrick BeisserCees NeefEllen Stobberingh Source Type: journals
Response of Bacillus cereus ATCC 14579 to challenges with sublethal concentrations of enterocin AS-48
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Conclusion:
BC4207 membrane protein is directly involved in the resistance mechanism of B. cereus cells against AS-48. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 28, 2009 Category: Microbiology Authors: Maria Grande BurgosAkos KovacsAleksandra MironczukHikmate AbriouelAntonio GalvezOscar Kuipers Source Type: journals
Evaluation of the bacterial diversity among and within individual venous leg ulcers using bacterial tag-encoded FLX and Titanium amplicon pyrosequencing and metagenomic approaches.
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Conclusions:
The polymicrobial nature of VLU and previous research on diabetic foot ulcers and surgical site infections suggest that the future of therapy for such wounds lies in the core of the logical and proven multiple concurrent strategy approach, which has been termed "biofilm-based wound care" and the use of individualized therapeutics rather than in a single treatment modality. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 27, 2009 Category: Microbiology Authors: Randall WolcottViktoria GontcharovaYan SunScot Dowd Source Type: journals
Isolation of Cronobacter spp. (formerly Enterobacter sakazakii) from infant food, herbs and environmental samples and the subsequent identification and confirmation of the isolates using biochemical, chromogenic assays, PCR and 16S rRNA sequencing
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Conclusion:
Our studies showed that Cronobacter spp. are highly diverse and share many phenotypic traits with other Enterobacteriaceae members highlighting the need to use several methods to confirm the identity of this pathogen. None of the biochemical, chromogenic or PCR primers proved to be a reliable method for confirmation of the identity of the isolates as all of them gave either false positives or false negatives or both. It is therefore concluded that 16S rRNA sequencing is pivotal to confirm the identity of the isolates. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 27, 2009 Category: Microbiology Authors: Ziad JaradatQotaiba AbabnehIsmail SaadounNawal SamaraAbrar Rashdan Source Type: journals
Multilocus sequence typing of Cronobacter sakazakii and Cronobacter malonaticus reveals stable clonal structures with clinical significance which do not correlate with biotypes.
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Conclusions:
In conclusion, MLST is a more robust means of identifying and discriminating between C. sakazakii and C. malonaticus than biotyping. The MLST database for these organisms is available online at http://pubmlst.org/cronobacter/. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 22, 2009 Category: Microbiology Authors: Adam BaldwinMichael LoughlinJuncal Caubilla-BarronEva KucerovaGeorgina ManningChristopher DowsonStephen Forsythe Source Type: journals
Regulation of phenylacetic acid degradation genes of Burkholderia cenocepacia K56-2
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Conclusions:
The PA catabolic genes of B. cenocepacia K56-2 are induced by PA and other related compounds, are negatively regulated by PaaR (named herein), a TetR-type regulator, and are subjected to catabolic repression by glucose and succinate. As the PA catabolic pathway of B. cenocepacia appears to be induced during growth in synthetic cystic fibrosis medium (SCFM), further research is necessary to determine the relevance of this pathway in CF-like conditions and in other host-pathogen interactions. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 17, 2009 Category: Microbiology Authors: Jason HamlinRuhi BloodworthSilvia Cardona Source Type: journals
Population diversity and antibody selective pressure to Plasmodium falciparum MSP1 block2 locus in an African malaria-endemic setting
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Conclusion:
The Pfmsp1 block2 locus presents a very large population sequence diversity. The lack of stable acquisition of novel antibody specificities despite exposure to novel allelic forms is reminiscent of clonal imprinting. The locus appears under antibody-mediated diversifying selection in a variable environment that maintains a balance between the various family types without selecting for sequence variant allelic forms. There is no evidence of positive selection for intra-family sequence diversity, consistent with the observed characteristics of the antibody response. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 14, 2009 Category: Microbiology Authors: Nitchakarn NoranateFranck PrugnolleHelene JouinAdama TallLaurence MarramaCheikh SokhnaMarie-Therese EkalaMicheline GuillotteEmmanuel BischoffChristiane BouchierJintana PatarapotikulJun OhashiJean-Francois TrapeChristophe RogierOdile Mercereau-Puijalon Source Type: journals
A novel inhibitor of Chlamydophila pneumoniae protein kinase D (PknD) inhibits phosphorylation of CdsD and suppresses bacterial replication
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Conclusion:
We have identified a 3'-pyridyl oxindole compound that inhibits the in vitro kinase activity of C. pneumoniae PknD and inhibits the growth and production of infectious C. pneumoniae progeny in HeLa cells. Together, these results suggest that PknD may play a key role in the developmental cycle of C. pneumoniae. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 13, 2009 Category: Microbiology Authors: Dustin JohnsonChris StoneDavid BulirBrian CoombesJames Mahony Source Type: journals
Gene expression analysis of the biocontrol fungus Trichoderma harzianum in the presence of tomato plants, chitin, or glucose using a high-density oligonucleotide microarray
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Conclusions:
The effectiveness of the Trichoderma HDO microarray to detect different gene responses under different growth conditions in the fungus T. harzianum strongly indicates that this tool should be useful for further assays that include different stages of plant colonization, as well as for expression studies in other Trichoderma spp. represented on it. Using this microarray, we have been able to define a number of genes probably involved in the transcriptional response of T. harzianum within the first hours of contact with tomato plant roots, which may provide new insights into the mechanisms and roles of this fung...
Source: BMC Microbiology - Latest articles - October 12, 2009 Category: Microbiology Authors: Ilanit SamolskiAlberto De LuisJ. Antonio VizcainoEnrique MonteM. Belen Suarez Source Type: journals
Environmental and intracellular regulation of Francisella tularensis ripA
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Conclusions:
Expression of ripA is required for growth at neutral pH, is pH sensitive, and is responsive to the intracellular environment. The intracellular expression pattern of ripA coincided with iglA, which is positively regulated by MglA and SspA. However, in contrast to their positive impact on iglA expression, MglA and SspA negatively impacted ripA expression in vitro. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 11, 2009 Category: Microbiology Authors: James FullerTodd KijekSharon Taft-BenzThomas Kawula Source Type: journals
Identification of genes coding for B cell antigens of Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC) by using phage display
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Conclusions:
Since phage display physically couples phenotype with genotype, it could be used to compile a list of sequences that code for MmmSC proteins bearing epitopes which were recognised by antibodies in the serum of an infected animal. Together with the appropriate bioinformatic analyses, this approach provided several potentially useful vaccine or diagnostic leads. The phage display step empirically identified sequences by their interaction with antibodies which accordingly reduced the number of ORFs that had to be expressed for testing. This is a particular advantage when working with MmmSC since the mycoplasmal c...
Source: BMC Microbiology - Latest articles - October 8, 2009 Category: Microbiology Authors: Dubravka MiltiadouArshad MatherEdy VileiDion du Plessis Source Type: journals
AI-2 does not Function as a Quorum Sensing Molecule in Campylobacter jejuni during exponential growth in vitro
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Conclusions:
Taken together these results led us to conclude that there is no evidence for the role of AI-2 in cell-to-cell communication in C. jejuni under the growth conditions used, and that the effects of the luxS mutation on the transcriptome are related to the consequential loss of function in the activated methyl cycle. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 7, 2009 Category: Microbiology Authors: Kathryn HolmesTimothy TavenderKlaus WinzerJerry WellsKim Hardie Source Type: journals
Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants
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Conclusions:
The results of this study showed that it is necessary to use multiple genotyping techniques targeting different sources of genetic variation to obtain the level of discrimination necessary to investigate transmission dynamics and trace the source of Map infections. Furthermore, the combination of genotyping techniques may depend on the geographical location of the population to be tested. Identical genotypes were obtained from Map isolated from different host species co-habiting on the same property strongly suggesting that interspecies transmission occurs. Interspecies transmission of Map between wildlife spe...
Source: BMC Microbiology - Latest articles - October 6, 2009 Category: Microbiology Authors: Karen StevensonJulio AlvarezDouwe BakkerFranck BietLucia de JuanSusan DenhamZoi DimareliKaren DohmannGerald-F. GerlachIan HeronMarketa KopecnaLinda MayIvo PavlikJ SharpVirginie ThibaultPeter WillemsenRuth ZadoksAlastair Greig Source Type: journals
Inactivation of staphylococcal virulence factors using a light-activated antimicrobial agent
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Conclusions:
These results suggest that photodynamic therapy could reduce the harmful impact of preformed virulence factors on the host. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 4, 2009 Category: Microbiology Authors: Sarah TubbyMichael WilsonSean Nair Source Type: journals
The effect of the macrolide antibiotic tylosin on microbial diversity in the canine small intestine as demonstrated by massive parallel 16S rDNA sequencing
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Background:
Recent studies have shown that the fecal microbiota is generally resilient to short-term antibiotic administration, but some bacterial taxa may remain depressed for several months. Limited information is available about the effect of antimicrobials on small intestinal microbiota, an important contributor to gastrointestinal health. The antibiotic tylosin is often successfully used for the treatment of chronic diarrhea in dogs, but its exact mode of action and its effect on the intestinal microbiota remain unknown. The aim of this study was to evaluate the effect of tylosin on canine jejunal microbiota. Tylosin ...
Source: BMC Microbiology - Latest articles - October 1, 2009 Category: Microbiology Authors: Jan SuchodolskiScot DowdElias WestermarckJorg SteinerRandy WolcottThomas SpillmannJaana Harmoinen Source Type: journals
Metabolic fluxes in the central carbon metabolism of Dinoroseobacter shibae and Phaeobacter gallaeciensis, two members of the marine Roseobacter clade
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Conclusions:
The present study reveals the first insight into pathway utilisation within the Roseobacter group. Fluxes through major intracellular pathways of the central carbon metabolism, which are closely linked to the various important traits found for the Roseobacter clade, could be determined. The close similarity of fluxes between the two physiologically rather different species might provide the first indication of more general key properties among members of the Roseobacter clade which may explain their enormous success in the marine realm. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 28, 2009 Category: Microbiology Authors: Tobias FurchMathias PreusseJurgen TomaschHajo ZechIrene Wagner-DoblerRalf RabusChristoph Wittmann Source Type: journals
Visualization of ribosomal RNA operon copy number distribution
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Conclusion:
Once the phylogenetic position of a novel organism is known the number of rRNA operons, and to a lesser extent the genome size, can be estimated by examination of the colored maps. Further detail can then be obtained for members of relevant taxa from the rrnDB database. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 24, 2009 Category: Microbiology Authors: Rajat RastogiMartin WuIndrani DasguptaGeorge Fox Source Type: journals
MLVA distribution characteristics of Yersinia pestis in China and the correlation analysis
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Conclusions:
The strains isolated from Yulong, Yunnan province had a unique MLVA type, indicating a new clone group. Our results suggest that Yulong strains may have a close relationship with strains from the Qinghai-Tibet Plateau plague focus. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 22, 2009 Category: Microbiology Authors: Xiaoai ZhangRong HaiJianchun WeiZhigang CuiEnmin ZhangZhizhong SongDongzheng Yu Source Type: journals
Genotypic comparison of Pantoea agglomerans plant and clinical strains
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Conclusion:
Taxonomic mischaracterization was identified as a major problem with P. agglomerans, and current techniques removed a majority of clinical strains from this species. Although clear discrimination between P. agglomerans plant and clinical strains was not obtained with phylogenetic analysis, a single marker characteristic of biocontrol strains was identified which may be of use in strain biosafety determinations. In addition, the lack of Koch's postulate fulfilment, rare retention of clinical strains for subsequent confirmation, and the polymicrobial nature of P. agglomerans clinical reports should be considered ...
Source: BMC Microbiology - Latest articles - September 21, 2009 Category: Microbiology Authors: Fabio RezzonicoTheo SmitsEmilio MontesinosJuerg FreyBrion Duffy Source Type: journals
Antimicrobial resistance profiling and molecular subtyping of Campylobacter spp. from processed turkey
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Conclusions:
Ciprofloxacin and erythromycin resistance in Campylobacter recovered from processed turkey occurred more frequently among C. coli than C. jejuni. Fla-PFGE types were associated with a particular species, antimicrobial resistance profiles, and a specific plant. Molecular subtyping in this study provided more information about the relationships among antimicrobial-resistant Campylobacter at the processing level. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 20, 2009 Category: Microbiology Authors: Ellen LutgenJohn McEvoyJulie SherwoodCatherine Logue Source Type: journals
Use of in vivo-induced antigen technology (IVIAT) for the identification of Streptococcus suis serotype 2 in vivo-induced bacterial protein antigens
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Conclusion:
Collectively, our results suggest that these in vivo-induced or upregulated genes may contribute to SS2 disease development. We hypothesize that the identification of factors specifically induced or upregulated during SS2 infection will aid in our understanding of SS2 pathogenesis and may contribute to the control SS2 outbreaks. In addition, the proteins identified using IVIAT may be useful potential vaccine candidates or virulence markers. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 17, 2009 Category: Microbiology Authors: Hongwei GuHaodan ZhuChengping Lu Source Type: journals
Assessment of three Resistance-Nodulation-Cell Division drug efflux transporters of Burkholderia cenocepacia in intrinsic antibiotic resistance
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Conclusions:
Two of the three inactivated RND efflux pumps in B. cenocepacia J2315 contribute to the high level of intrinsic resistance of this strain to some antibiotics and other inhibitory compounds. Furthermore, these efflux systems also mediate accumulation in the growth medium of quorum sensing molecules that have been shown to contribute to infection. A systematic study of RND efflux systems in B. cenocepacia is required to provide a full picture of intrinsic antibiotic resistance in this opportunistic bacterium. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 16, 2009 Category: Microbiology Authors: Silvia BuroniMaria PascaRonald FlannaganSilvia BazziniAnna MilanoIris BertaniVittorio VenturiMiguel ValvanoGiovanna Riccardi Source Type: journals
High-level chromate resistance in Arthrobacter sp. strain FB24 requires previously uncharacterized accessory genes
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Conclusions:
Our findings indicate that chromate resistance in Arthrobacter sp. strain FB24 is due to chromate efflux through the ChrA transport protein. More importantly, new genes have been identified as having significant roles in chromate resistance. Collectively, the functional predictions of these additional genes suggest the involvement of a signal transduction system in the regulation of chromate efflux and warrants further study. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 15, 2009 Category: Microbiology Authors: Kristene HenneCindy NakatsuDorothea ThompsonAllan Konopka Source Type: journals
Outer membrane vesicles of helicobacter pylori TK1402 are involved in biofilm formation
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Conclusion:
The results suggested that OMV produced from TK1402 play an important role in biofilm formation in strain TK1402. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 14, 2009 Category: Microbiology Authors: Hideo YonezawaTakako OsakiSatoshi KurataMinoru FukudaHayato KawakamiKuniyasu OchiaiTomoko HanawaShigeru Kamiya Source Type: journals
First multi-locus sequence typing scheme for Arcobacter spp.
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Conclusions:
No association of MLST alleles or sequence types with host or geographical source was observed with this sample set. Nevertheless, the large number of identified alleles and sequence types indicate that this MLST method will prove useful in both Arcobacter strain discrimination and in epidemiological studies of sporadic Arcobacter-related gastroenteritis. A new Arcobacter MLST database was created (http://pubmlst.org/arcobacter/); allele and ST data generated in this study were deposited in this database and are available online. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 13, 2009 Category: Microbiology Authors: William MillerIrene WesleyStephen OnKurt HoufFrancis MegraudGuilin WangEmma YeeApichai SrijanCarl Mason Source Type: journals
Mycoplasma pneumoniae and/or Chlamydophila pneumoniae inoculation causing different aggravations in cholesterol-induced atherosclerosis in apoE KO male mice
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Conclusion:
Inoculation of CP, MP or both agents in C57BL/6 apoE KO male mice caused aggravation of experimental atherosclerosis induced by cholesterol-enriched diet, with distinct characteristics. CP inoculation increased the plaque height with positive vessel remodeling and co-inoculation of MP + CP caused the highest adventitial inflammation measures. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 9, 2009 Category: Microbiology Authors: Sueli DamyMaria HiguchiJorge TimenetskyMarcia ReisSuely PalominoRenata IkegamiFabiana SantosJunko OsakaLuis Figueiredo Source Type: journals
Structural analysis of the full-length gene encoding a fibronectin-binding-like protein (CadF) and its adjacent genetic loci within Campylobacter lari
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Conclusions:
The putative cadF ORFs from all C. lari isolates were nine amino acid larger than those from C. jejuni, and showed amino acid residues 137 -140 of FALG (50% identity), instead of the FRLS residues of the maximal fibronectin-binding activity site demonstrated within C. jejuni CadF. A neighbor joining tree constructed based on cadF gene sequence information formed a major cluster consisting of C. lari isolates, separating from the other three thermophilic campylobacters (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 7, 2009 Category: Microbiology Authors: Junichi HirayamaTsuyoshi SekizukaAkihiro TazumiIkue TaneikeJohn MooreB. Cherie MillarMotoo Matsuda Source Type: journals
Outer membrane porin M35 of Moraxella catarrhalis mediates susceptibility to aminopenicillins
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Background:
The outer membrane protein M35 is an conserved porin of type 1 strains of the respiratory pathogen Moraxella catarrhalis. It was previously shown that M35 is involved in the uptake of essential nutrients required for bacterial growth and for nasal colonization in mice. The aim of this study was (i) to characterize the potential roles of M35 in the host-pathogen interactions considering the known multifunctionality of porins and (ii) to characterize the degree of conservation in the phylogenetic older subpopulation (type 2) of M. catarrhalis.
Results:
Isogenic m35 mutants of the type 1 strains O35E, 300 and 415 ...
Source: BMC Microbiology - Latest articles - September 3, 2009 Category: Microbiology Authors: Marion JetterNadja HeinigerVioleta SpaniolRolf TrollerAndre SchallerChristoph Aebi Source Type: journals
Bactericidal activities of the cationic steroid CSA-13 and the cathelicidin peptide LL-37 against Helicobacter pylori in simulated gastric juice.
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Conclusion:
These data indicate that cholic acid-based antimicrobial agents such as CSA-13 resist proteolytic degradation and inhibition by mucin and have potential for treatment of H. pylori infections, including those caused by the clarithromycin and/or metronidazole-resistant strains. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - September 2, 2009 Category: Microbiology Authors: Katarzyna LeszczynskaAndrzej NamiotDavid FeinQi WenZbigniew NamiotPaul SavageScott DiamondPaul JanmeyRobert Bucki Source Type: journals
Identification of two GH18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete Aphanomyces astaci
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Conclusion:
The simultaneous qualitative detection of multiple sequences by qPCR/MCA represents a promising approach to detect species with elevated levels of genetic variation and/or limited available sequence information. The homogenous closed-tube format, reduced detection time, higher specificity, and the considerably reduced chance of false-negative detection achieved by multiplexing the amplification of functionally constrained target genes (CHI2, CHI3) with an endogenous control are the major advantages of this multiplex assay compared to other diagnostic methods.Sensitive quantification achieved with TaqMan qPCR fa...
Source: BMC Microbiology - Latest articles - August 30, 2009 Category: Microbiology Authors: Gerald HochwimmerReinhard ToberRene Bibars-ReiterElisabeth LicekRalf Steinborn Source Type: journals
Characterization of a salt-induced DhAHP, a gene coding for alkyl hydroperoxide reductase, from the extremely halophilic yeast Debaryomyces hansenii
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Conclusions:
In conclusion, for the first time our study has identified alkly hydroperoxide reductase as a key protein involved in the salt tolerance of the extremely halophilic D. hansenii. Apparently, this enzyme plays a multi-functional role in the yeast's adaptation to salinity; it serves as a peroxidase in scavenging reactive oxygen species, as a molecular chaperone in protecting essential proteins from denaturation, and as a redox sensor in regulating H2O2-mediated cell defense signaling. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 27, 2009 Category: Microbiology Authors: Hsiu-fung ChaoYung-fu YenMaurice Ku Source Type: journals
Role of the ArcAB two-component system in the resistance of Escherichia coli to reactive oxygen stress
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Conclusions:
Our results suggest that in addition to its role as a global regulator for anaerobic growth of bacteria, ArcAB system is also important for bacterial resistance to ROS in aerobic conditions, possibly through its influence on bacterial metabolism, especially amino acid and/or protein assimilation and synthesis. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 27, 2009 Category: Microbiology Authors: Cindy LouiAlexander ChangSangwei Lu Source Type: journals
Extracellular secretion of Carocin S1 in Pectobacterium carotovorum subsp. carotovorum occurs via the type III secretion system integral to the bacterial flagellum
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Conclusions:
Thus, the extracellular export of Carocin S1 by Pectobacterium carotovorum subsp. carotovorum appears to utilize the type III secretion system integral to bacterial flagella. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 26, 2009 Category: Microbiology Authors: Yung-Chieh ChanHuang-Pin WuDuen-yau Chuang Source Type: journals
Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis
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Conclusion:
The sycO-ypkA-yopJ operon is under the direct and negative regulation of CRP in Y. pestis. The sycO-ypkA-yopJ promoter-proximate regions are extremely conserved in Y. pestis, Y. pseudotuberculosis and Y. enterocolitica. Therefore, data presented here can be generally applied to the above three pathogenic yersiniae. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 24, 2009 Category: Microbiology Authors: Lingjun ZhanLei YangLei ZhouYingli LiHe GaoZhaobiao GuoLiangfeng ZhangChuan QinDongsheng ZhouRuifu Yang Source Type: journals
Involvement of SPI-2-encoded SpiC in flagellum synthesis in Salmonella enterica serovar Typhimurium
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Conclusion:
The data show that SpiC is involved in flagella assembly by affecting the post-transcription expression of flhDC. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 24, 2009 Category: Microbiology Authors: Kei-ichi UchiyaAsami SugitaToshiaki Nikai Source Type: journals
Melanin is an essential component for the integrity of the cell wall of Aspergillus fumigatus conidia
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Conclusions:
These results suggest that, in addition to a protective role against the host's immune defences, melanin is also a structural component of the conidial wall that is required for correct assembly of the cell wall layers and the expression at the conidial surface of adhesins and other virulence factors. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 23, 2009 Category: Microbiology Authors: Marc PihetPatrick VandeputteGuy TronchinGilles RenierPatrick SaulnierSonia GeorgeaultRomain MalletDominique ChabasseFrancoise SymoensJean-Philippe Bouchara Source Type: journals
Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population
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Conclusions:
Pre-EPIYA-region typing of the cagA gene could provide a new genetic marker of H. pylori genomic diversity. Our data support the hypothesis that vacA m1 is closely associated with gastric carcinogenesis. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 22, 2009 Category: Microbiology Authors: Tomohisa UchidaLam Tung NguyenAkiko TakayamaTadayoshi OkimotoMasaaki KodamaKazunari MurakamiTakeshi MatsuhisaTuan Dung TrinhLong TaDang Quy Dung HoHoa Hai HoangTetsuko KishidaToshio FujiokaMasatsugu MoriyamaYoshio Yamaoka Source Type: journals
Organic acid production in vitro and plant growth promotion in maize under controlled environment by phosphate-solubilizing fluorescent Pseudomonas
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Conclusions:
The results implied that organic acid production by Pseudomonas strains is independent of their genetic relatedness and each strain has its own ability of producing organic acids during the solubilization of inorganic phosphates. Significant difference in plant growth promotion by efficient phosphate-solubilizing Pseudomonas strains point at the need for selecting potential strains in plant growth promotion experiments in conjunction with various phosphate substrates for their targeted application as bioinoculants. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 21, 2009 Category: Microbiology Authors: Pratibha VyasArvind Gulati Source Type: journals
CgOpt1, a putative oligopeptide transporter from Colletotrichum gloeosporioides that is involved in responses to auxin and pathogenicity
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Conclusions:
Our results show that IAA induces developmental changes in C. gloeosporioides. These changes are blocked in cgopt1-silenced mutants, suggesting that this protein is involved in regulation of fungal response to IAA. CgOPT1 is also necessary for full virulence, but it is unclear whether this phenotype is related to auxin. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 20, 2009 Category: Microbiology Authors: Veroniue ChagueRudy MaorAmir Sharon Source Type: journals
Immunoproteomic analysis of outer membrane proteins and extracellular proteins of Actinobacillus pleuropneumoniae JL03 serotype 3
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Conclusions:
These data provide information about novel immunogenic proteins of A. pleuropneumoniae, and are expected to aid in development of novel vaccines against A. pleuropneumoniae. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 19, 2009 Category: Microbiology Authors: Yonghong LiaoJunhua DengAnding ZhangMingguang ZhouYong HuHuanchun ChenMeilin Jin Source Type: journals
msbB deletion confers acute sensitivity to CO2 in Salmonella enterica serovar Typhimurium that can be suppressed by a loss-of-function mutation in zwf
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Conclusions:
These results demonstrate that msbB confers acute sensitivity to CO2, acidic pH, and high osmolarity. Disruption of zwf in msbB mutants restores growth in 5% CO2 and results in improved growth in acidic media or in media with high osmolarity. These results add to a growing list of phenotypes caused by msbB and mutations that suppress specific growth defects. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 17, 2009 Category: Microbiology Authors: Verena KarstenSean MurrayJeremy PikeKimberly TroyMartina IttensohnManvel KondradzhyanK LowDavid Bermudes Source Type: journals
Rapid detection of ERG11 gene mutations in clinical Candida albicans isolates with reduced susceptibility to fluconazole by rolling circle amplification and DNA sequencing
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Conclusions:
The sensitive RCA assay described here is a simple, robust and rapid (2 h) method for the detection of ERG11 polymorphisms. It showed excellent concordance with ERG11 sequencing and is a potentially valuable tool to track the emergence and spread of azole-resistant C. albicans and to study the epidemiology of ERG11 mutations. The RCA method is applicable to the study of azole resistance in other fungi. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 13, 2009 Category: Microbiology Authors: Huiping WangFanrong KongTania SorrellBin WangPaul McNicholasNamfon PantaratDavid EllisMeng XiaoFred WidmerSharon Chen Source Type: journals
Persistence of colicinogenic Escherichia coli in the mouse gastrointestinal tract
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Conclusions:
The data presented here support prior claims that bacteriocin production may play a significant role in the colonization of E. coli in the gastrointestinal tract. Further, this study suggests that the ability to produce bacteriocins may prove to be a critical factor in determining the success of establishing probiotic E. coli in the gastrointestinal tract of humans and animals. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 11, 2009 Category: Microbiology Authors: Osnat GillorItamar GiladiMargaret Riley Source Type: journals
A single amino acid change (Y318F) in the L-arabitol dehydrogenase (LadA) from Aspergillus niger results in a significant increase in affinity for D-sorbitol
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Conclusions:
These data demonstrates that Y318 of LadA contributes significantly to the substrate specificity difference between LAD and XDH/SDH. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - August 11, 2009 Category: Microbiology Authors: Lucy RuttenCecile RibotBlanca Trejo-AguilarHan WostenRonald de Vries Source Type: journals
