BMC Plant Biology - Latest articles
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Genome-wide analysis of major intrinsic proteins in the tree plant Populus trichocarpa: Characterization of XIP subfamily of aquaporins from evolutionary perspective
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Conclusions:
Due to whole genome duplication, Populus has the largest number of MIPs identified in any single species. Non-XIP MIPs are similar in all four plant species considered in this study. Small and weakly polar residues at the helix-helix interface are group conserved presumably to maintain the hourglass fold of MIP channels. Substitutions in ar/R selectivity filter, insertion/deletion in loop C, increasing basic nature of loop D and loss of introns are some of the events occurred during the evolution of dicot XIPs. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - November 20, 2009 Category: Biology Authors: Anjali GuptaRamasubbu Sankararamakrishnan Source Type: journals
In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots
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Conclusions:
We have produced a comprehensive atlas of TIP expression in Arabidopsis roots, which reveals novel expression patterns for not previously studied TIPs. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - November 18, 2009 Category: Biology Authors: Stefano GattolinMathias SorieulPaul HunterRoman KhonsariLorenzo Frigerio Source Type: journals
Genome scale transcriptome analysis of shoot organogenesis in Populus
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Conclusions:
The major regulatory events during plant cell organogenesis occur at early stages of dedifferentiation. The regulatory circuits reflect the combinational effects of transcriptional control and hormone signaling, and associated changes in light environment imposed during dedifferentiation. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - November 17, 2009 Category: Biology Authors: Yanghuan BaoPalitha DharmawardhanaTodd MocklerSteven Strauss Source Type: journals
Comparative analysis of the complete sequence of the plastid genome of Parthenium argentatum and identification of DNA barcodes to differentiate Parthenium species and lines
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Conclusion:
The genome sequence of the P. argentatum chloroplast will enrich the sequence resources of plastid genomes in commercial crops. The availability of the complete plastid genome sequence may facilitate transformation efficiency by using the precise sequence of endogenous flanking sequences and regulatory elements in chloroplast transformation vectors. The DNA barcoding study forms the foundation for genetic identification of commercially significant lines of P. argentatum that are important for producing latex. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - November 17, 2009 Category: Biology Authors: Shashi KumarFrederick HahnColleen McMahanKatrina CornishMaureen Whalen Source Type: journals
Vitamin B6 deficient plants display increased sensitivity to high light and photo-oxidative stress
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Conclusions:
This study demonstrates that vitamin B6 has a function in the in vivo antioxidant defense of plants. Thus, the antioxidant activity of vitamin B6 inferred from in vitro studies is confirmed in planta. Together with the finding that chloroplasts contain vitamin B6 compounds, the data show that vitamin B6 functions as a photoprotector that limits 1O2 accumulation in high light and prevents 1O2-mediated oxidative damage. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - November 10, 2009 Category: Biology Authors: Michel HavauxBrigitte KsasAgnieszka SzewczykDominique RumeauFabrice FranckStefano CaffarriChristian Triantaphylides Source Type: journals
Pollen development in Annona cherimola Mill. (Annonaceae). Implications for the evolution of aggregated pollen
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Conclusion:
Pollen grouping could be the result of relatively minor ontogenetic changes beneficial for pollen transfer or/and protection from desiccation. Comparison of these events with those recorded in the recent pollen developmental mutants in Arabidopsis indicates that several failures during tetrad dissolution may convert to a common recurring phenotype that has evolved independently several times, whenever this grouping conferred advantages for pollen transfer. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - October 29, 2009 Category: Biology Authors: Jorge LoraPilar TestillanoMaria RisuenoJose HormazaMaria Herrero Source Type: journals
Computational annotation of genes differentially expressed along olive fruit development
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Conclusions:
The bioinformatic annotation of all gene sequences was useful to shed light on metabolic pathways and transcriptional aspects related to carbohydrates, fatty acids, secondary metabolites, transcription factors and hormones as well as response to biotic and abiotic stresses throughout olive drupe development. These results represent a first step toward both functional genomics and systems biology research for understanding the gene functions and regulatory networks in olive fruit growth and ripening. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - October 23, 2009 Category: Biology Authors: Giulio GallaGianni BarcacciaAngelo RaminaSilvio CollaniFiammetta AlagnaLuciana BaldoniNicolo CultreraFederico MartinelliLuca SebastianiPietro Tonutti Source Type: journals
Comparative transcriptional survey between laser-microdissected cells from laminar abscission zone and petiolar cortical tissue during ethylene-promoted abscission in citrus leaves
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Conclusion:
The LCM-based data generated in this survey represent the most accurate description of the main biological processes and genes involved in organ abscission in citrus. This study provides novel molecular insight into ethylene-promoted leaf abscission and identifies new putative target genes for characterization and manipulation of organ abscission in citrus. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - October 22, 2009 Category: Biology Authors: Javier AgustiPaz MereloManuel CercosFrancisco TadeoManuel Talon Source Type: journals
Seed storage protein gene promoters contain conserved DNA motifs in Brassicaceae, Fabaceae and Poaceae
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Conclusions:
Seed storage protein gene promoter motifs are conserved in diverse species, and different plant families are characterized by a distinct combination of conserved motifs. The majority of discovered motifs match experimentally characterized cis-regulatory elements. These results provide a good starting point for further experimental analysis of plant seed-specific promoters and our methodology can be used to unravel more transcriptional regulatory mechanisms in plants and other eukaryotes. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - October 19, 2009 Category: Biology Authors: Francois FauteuxMartina Stromvik Source Type: journals
Sampling nucleotide diversity in cotton
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Conclusion:
In this research, SNP and indel diversity is characterized for 270 single-copy polymorphic loci in cotton. A strategy for SNP discovery is defined to pre-screen loci for copy number and polymorphism. Our data indicate that the A and D genomes in both diploid and tetraploid cotton remain distinct from each such that homoeologs can be distinguished. This research provides mapped DNA markers for intra-specific crosses and introgression of exotic germplasm in cotton. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - October 19, 2009 Category: Biology Authors: Allen Van DeynzeKevin StoffelMike LeeThea WilkinsAlexander KozikRoy CantrellJohn YuRussell KohelDavid Stelly Source Type: journals
Phenolic compounds in ectomycorrhizal interaction of lignin modified silver birch
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Conclusions:
The down-regulation of BpCOMT in the 35S-PtCOMT lines caused a reduction in the syringyl/quaiacyl ratio of lignin, but no significant effect was seen in the composition or quantity of phenolic compounds that would have been caused by the expression of PtCOMT under the 35S or UbB1 promoter. Moreover, the detected alterations in the composition of lignin and secondary phenolic compounds had no effect on the interaction between silver birch and P. involutus. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - September 28, 2009 Category: Biology Authors: Suvi SutelaKaroliina NiemiJaanika EdesiTapio LaaksoPekka SaranpaaJaana VuoskuRiina MakelaHeidi TiimonenVincent ChiangJanne KoskimakiMarja SuorsaRiitta Julkunen-TiittoHely Haggman Source Type: journals
MoccaDB - an integrative database for functional, comparative and diversity studies in the Rubiaceae family
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Conclusion:
We believe that MoccaDB will be extremely useful for all researchers working in the areas of comparative and functional genomics and molecular evolution, in general, and population analysis and association mapping of Rubiaceae and Solanaceae species, in particular. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - September 28, 2009 Category: Biology Authors: Olga PlechakovaChristine Tranchant-DubreuilFabrice BenedetMarie CoudercAlexandra TinautVeronique ViaderPetra De BlockPerla HamonClaudine CampaAlexandre de KochkoSerge HamonValerie Poncet Source Type: journals
Identification of flowering genes in strawberry, a perennial SD plant
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Conclusions:
We have shown that many central genetic components of the flowering pathways in Arabidopsis can be identified from strawberry. However, novel regulatory mechanisms exist, like SFL that functions as a switch between short-day/low temperature and long-day/high temperature flowering responses between the short-day genotype and the everbearing 'Baron Solemacher'. The identification of putative flowering gene homologs and AP1 as potential marker gene for floral initiation will strongly facilitate the exploration of strawberry flowering pathways. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - September 27, 2009 Category: Biology Authors: Katriina MouhuTimo HytonenKevin FoltaMarja RantanenLars PaulinPetri AuvinenPaula Elomaa Source Type: journals
The Hawaiian algal database: a laboratory LIMS and online resource for biodiversity data
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Conclusions:
The Hawaiian Algal Database is a digital repository for Hawaiian algal samples and acts as a LIMS for the laboratory. Users can make use of the online search tool to view and download specimen photographs and micrographs, DNA sequences and relevant habitat data, including georeferenced collecting locations. It is publicly available at http://algae.manoa.hawaii.edu. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - September 3, 2009 Category: Biology Authors: Norman WangAlison SherwoodAkira KuriharaKimberly ConklinThomas SauvageGernot Presting Source Type: journals
Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus (daikon radish)
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Conclusions:
The present findings suggest that HMT/HTMT activity is present in several families of higher plants including Poaceae and Brassicaceae, and is involved in the formation of methyl halides. Moreover, it was found that the emission of methyl iodide from plants was affected by the iodide concentration in the cultures. The recombinant RsHTMT demonstrated enzymatic properties similar to those of Brassica oleracea HTMT, especially in terms of its high specificity for iodide, bisulfide, and thiocyanate ions. A survey of biogenic emissions of methyl halides strongly suggests that the HTM/HTMT reaction is the key to und...
Source: BMC Plant Biology - Latest articles - August 31, 2009 Category: Biology Authors: Nobuya ItohHiroshi TodaMichiko MatsudaTakashi NegishiTomokazu TaniguchiNoboru Ohsawa Source Type: journals
A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat
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Conclusions:
We have generated reverse genetics TILLING resources for pasta and bread wheat and achieved a high mutation density in both populations. We also developed a modified screening method that will lower barriers to adopt this promising technology. We hope that the use of this reverse genetics resource will enable more researchers to pursue wheat functional genomics and provide novel allelic diversity for wheat improvement. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 27, 2009 Category: Biology Authors: Cristobal UauyFrancine ParaisoPasqualina ColasuonnoRobert TranHelen TsaiSteve BerardiLuca ComaiJorge Dubcovsky Source Type: journals
Mapping quantitative trait loci (QTLs) for fatty acid composition in an interspecific cross of oil palm
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Conclusion:
This study describes the first successful detection of QTLs for fatty acid composition in oil palm. These QTLs constitute useful tools for application in breeding programmes. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 25, 2009 Category: Biology Authors: Rajinder SinghSoon TanJothi PanandamRahimah RahmanLeslie OoiEng-Ti LowMukesh SharmaJohannes JansenSuan-Choo Cheah Source Type: journals
Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis
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Conclusion:
This work provides a first characterization of the proteome changes that occur in response to nitrate availability in leaves and roots of maize plants. According to previous studies, the work confirms the relationship between nitrogen and carbon metabolisms and it rises some intriguing questions, concerning the possible role of NO and lipoxygenase 10 in roots and leaves, respectively. Although further studies will be necessary, this proteomic analysis underlines the central role of post-translational events in modulating pivotal enzymes, such as PEPCase. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 22, 2009 Category: Biology Authors: Bhakti PrinsiAlfredo NegriPaolo PesaresiMaurizio CocucciLuca Espen Source Type: journals
Recovery and characterization of a Citrus clementinaHort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence
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Conclusion:
The first haploid plant of 'Clemenules' clementine produced directly by germination of a haploid embryo, which grew vigorously and produced flowers, has been obtained in this work. This haploid line has been selected and it is being used by the ICGC to establish the reference sequence of the nuclear genome of citrus. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 21, 2009 Category: Biology Authors: Pablo AlezaJose JuarezMaria HernandezJose PinaPatrick OllitraultLuis Navarro Source Type: journals
Recovery and characterization of a Citrus clementina Hort ex Tan Clemenules haploid plant selected to establish the reference whole Citrus genome sequence
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Conclusions:
The first haploid plant of clementine Clemenules produced directly by germination of a haploid embryo that grew vigorously and produced flowers has been obtained from this work. This haploid line has been selected and it is being used by the ICGC to establish the reference sequence of the nuclear genome of citrus. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 21, 2009 Category: Biology Authors: Pablo AlezaJose JuarezMaria HernandezJose PinaPatrick OllitraultLuis Navarro Source Type: journals
A novel method for efficient and abundant production of Phytophthora brassicae zoospores on Brussels sprout leaf discs
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Conclusion:
The in vitro leaf disc method established from P. brassicae infected Brussels sprout leaves facilitates convenient and high-throughput production of infectious zoospores and is thus suitable to drive small and large scale inoculation experiments. The system has the advantage that zoospores are produced continuously over a period of at least one month. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 21, 2009 Category: Biology Authors: Klaas BouwmeesterFrancine Govers Source Type: journals
Identification of candidate genome regions controlling disease resistance in Arachis.
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Conclusions:
Candidate genes and QTLs were distributed on all linkage groups except for the smallest, but the distribution was not even. Groupings of candidate genes and QTLs for late leaf spot resistance were apparent on the upper region of linkage group 4 and the lower region of linkage group 2, indicating that these regions are likely to control disease resistance. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 21, 2009 Category: Biology Authors: Soraya Leal-BertioliAna Carolina JoseDione Alves-FreitasMarcio MoretzsohnPatricia GuimaraesStephan NielenBruna VidigalRinaldo PereiraJodie PikeAlessandra FaveroMartin ParniskeRajeev VarshneyDavid Bertioli Source Type: journals
Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation
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Conclusions:
Findings presented here suggest that CsGT45 is an active enzyme that plays a role in the formation of flavonoid glucosides in C. sativus. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 19, 2009 Category: Biology Authors: Angela Rubio-MoragaAlmudena Trapero MozosOussama AhrazemLourdes Gomez-Gomez Source Type: journals
Modification of tobacco plant development by sense and antisense expression of the tomato viroid-induced AGC VIIIa protein kinase PKV suggests involvement in gibberellin signaling
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Conclusion:
We have found that the over expression of the tomato protein kinase PKV resulted in stunting, modified vascular tissue development, reduced root formation, and male sterility in tobacco, and we propose that PKV regulates plant development by functioning in critical signaling pathways involved in gibberellic acid metabolism. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 17, 2009 Category: Biology Authors: Rosemarie HammondYan Zhao Source Type: journals
Comparative gene expression profiles between heterotic and non-heterotic hybrids of tetraploid Medicago sativa
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Conclusions:
The higher proportions of probe sets with expression level that differed from the parental midparent value and that were more extreme than either parental value in the heterotic hybrids compared to a non-heterotic hybrid were also found using RMA. We conclude that nonadditive expression of transcript levels may contribute to heterosis for biomass yield in alfalfa. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 12, 2009 Category: Biology Authors: Xuehui LiYanling WeiDan NettletonE. Charles Brummer Source Type: journals
Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca) BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome
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Conclusion:
We report the first targeted cloning, sequencing, assembly, and annotation of large segments of genomic DNA from a conifer. We demonstrate that genomic BAC clones for individual members of multi-member gene families can be isolated in a gene-specific fashion. The results of the present work provide important new information about the structure and content of conifer genomic DNA that will guide future efforts to sequence and assemble conifer genomes. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 5, 2009 Category: Biology Authors: Bjoern HambergerDawn HallMack YuenClaire OddyBritta HambergerChristopher KeelingCarol RitlandKermit RitlandJorg Bohlmann Source Type: journals
Gene expression profiling in susceptible interaction of grapevine with its fungal pathogen Eutypa lata: Extending MapMan ontology for grapevine
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Conclusions:
The extension of MapMan ontology to grapevine together with the newly constructed pictorial representations for carotenoid, terpenoid and phenylpropanoid metabolism provide an alternative approach to the analysis of grapevine gene expression experiments performed with Affymetrix or Operon microarrays. MapMan was first validated on an already published dataset and later used to obtain an overview of transcriptional changes in a susceptible grapevine - Eutypa lata interaction at the time of symptoms development, where we showed that the responsive genes belong to families known to be involved in the plant defenc...
Source: BMC Plant Biology - Latest articles - August 4, 2009 Category: Biology Authors: Ana RotterCeline CampsMarc LohseChristian KappelStefania PilatiMatjaz HrenMark StittPierre Coutos-ThevenotClaudio MoserBjorn UsadelSerge DelrotKristina Gruden Source Type: journals
Systemic acquired resistance in soybean is regulated by two proteins, Orthologous to Arabidopsis NPR1
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Conclusions:
Complementation analyses in Arabidopsis npr1-1 mutants revealed that homoeologous GmNPR1-1 and GmNPR1-2 genes are orthologous to Arabidopsis NPR1. Therefore, SAR pathway in soybean is most likely regulated by GmNPR1 genes. Substitution of Cys216 residue, essential for oligomer-monomer transition of Arabidopsis NPR1, with Ser and Leu residues in GmNPR1-1 and GmNPR1-2, respectively, suggested that there may be differences between the regulatory mechanisms of GmNPR1 and Arabidopsis NPR proteins. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 4, 2009 Category: Biology Authors: Devinder SandhuI. Made TasmaRyan FraschMadan Bhattacharyya Source Type: journals
Genetic mapping of wild introgressions into cultivated peanut: a way toward enlarging the genetic basis of a recent allotetraploid.
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Conclusions:
The genetic map developed, allowed the synteny analysis of the A and B genomes, the comparison with diploid and tetraploid maps and the analysis of the introgression segments from the wild synthetic into the background of a cultivated variety. The material we have produced in this study should facilitate the development of advanced backcross and CSSL breeding populations for the improvement of cultivated peanut. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - August 2, 2009 Category: Biology Authors: Daniel FoncekaTossim Hodo-AbaloRonan RivallanIssa FayeMbaye Ndoye SallOusmane NdoyeAlessandra FaveroDavid BertioliJean-Christophe GlaszmannBrigitte CourtoisJean-Francois Rami Source Type: journals
The cellulose synthase superfamily in fully sequenced plants and algae
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Conclusions:
Our data suggest that the CslA and CslC families originated through an ancient gene duplication event in land plants. We speculate that the single-copy Csl gene in green algae may encode a mannan synthase. We confirm that the rest of the Csl families have a different evolutionary origin than CslA and CslC, and have proposed a model for the divergence order among them. Our study provides new insights about the evolution of this important gene family in plants. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 30, 2009 Category: Biology Authors: Yanbin YinJinling HuangYing Xu Source Type: journals
Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgaris L.).
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Conclusions:
The present study provides a set of validated gene-based markers for common bean that are derived from G19833, an Andean landrace that is an important source of disease and abiotic stress tolerance which has been used for physical map development and as a mapping parent. Gene-based markers appear to be very efficient at separating divergent wild and cultivated accessions as well as Andean and Mesoamerican genepools and therefore will be useful for diversity analyses and for comparative and transcript mapping in common bean. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 30, 2009 Category: Biology Authors: Matthew BlairMonica Munoz TorresMartha GiraldoFabio Pedraza Source Type: journals
An archived activation tagged population of Arabidopsis thaliana to facilitate forward genetics approaches
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Conclusions:
This publicly available population provides an additional tool for plant researcher's to assist with determining gene function for the many as yet uncharacterised genes annotated within the Arabidopsis genome sequence (http://aafc-aac.usask.ca/FST). The presence of enhancer elements on the inserted T-DNA element allows both knock-out and dominant activation phenotypes to be identified for traits of interest. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 30, 2009 Category: Biology Authors: Stephen RobinsonLily TangBrent MooneySheldon McKayWayne ClarkeMatthew LinksSteven KarczSharon ReganYun-Yun WuMargaret GruberDejun CuiMin YuIsobel Parkin Source Type: journals
Mass spectrometry analysis of the variants of histone H3 and H4 of soybean and their post-translational modifications
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Conclusions:
This work revealed several distinct variants of soybean histone and their modifications that were different from A. thaliana, thus providing important biological information toward further understanding of the histone modifications and their functional significance in higher plants. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 30, 2009 Category: Biology Authors: Tao WuTiezheng YuanSau-Na TsaiChunmei WangSai-Ming SunHon-Ming LamSai-Ming Ngai Source Type: journals
Arabidopsis WRKY2 transcription factor mediates seed germination and postgermination arrest of development by abscisic acid
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Conclusions:
ABA hypersensitivity of the wrky2 mutants during seed germination and postgermination early seedling establishment is attributable to elevated mRNA levels of ABI5, ABI3 and ABI5-induced Em1 and Em6 in the mutants. WRKY2-mediated ABA responses are independent of miR159 and its target genes MYB33 and MYB101. ABI5, ABI3, ABA2 and ABA3 are important regulators of the transcripts of WRKY2 by ABA treatment. Our results suggest that WRKY2 transcription factor mediates seed germination and postgermination developmental arrest by ABA. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 21, 2009 Category: Biology Authors: Wenbo JiangDiqiu Yu Source Type: journals
Interactions between cauliflower and Rhizoctonia anastomosis groups with different levels of pathogenicity
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Conclusions:
The differences in pathogenicity towards cauliflower observed among Rhizoctonia AGs correlated with the infection rate, induction of host defence responses and pectin breakdown. All Rhizoctonia AGs studied were able to penetrate the plant tissue, indicating constitutive barriers of cauliflower were defeated and differences in pathogenicity were caused by inducible defence responses, including cell wall fortifications with phenolic compounds and callose. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 20, 2009 Category: Biology Authors: Joke PannecoucqueMonica Hofte Source Type: journals
Identification of an extensive gene cluster among a family of PPOs in Trifolium pratense L. (red clover) using a large insert BAC library
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Conclusions:
A PPO gene family in red clover resides as a cluster of at least 6 genes. Three of these genes have high homology, suggesting a more recent evolutionary event. This PPO cluster covers a longer region of the genome than clusters detected in rice or previously reported in tomato. Full-length coding sequences from PPO4, PPO5, PPO1/5 and PPO1/4 will facilitate functional studies and provide genetic markers for plant breeding. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 19, 2009 Category: Biology Authors: Ana WintersSue HeywoodKerrie FarrarIain DonnisonAnn ThomasK Judith Webb Source Type: journals
Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin
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Conclusion: Taken together, the results indicate that a diverse group of globulins exists in wheat, some of which could be associated with the pathogenesis of T1D in some susceptible individuals. These data expand our knowledge of specific wheat globulins and will enable further elucidation of their role in wheat biology and human health. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 16, 2009 Category: Biology Authors: Evelin LoitCharles MelnykAmanda MacFarlaneFraser ScottIllimar Altosaar Source Type: journals
A set of EST-SNPs for map saturation and cultivar identification in melon
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Conclusion:
This is the first example of a genetic map in a cucurbit species that includes a major set of SNP markers discovered using ESTs. The PI161375 x 'Piel de sapo' melon genetic map has around 700 markers, of which more than 500 are gene-based markers (SNP, RFLP and SSR). This genetic map will be a central tool for the construction of the melon physical map, the step prior to sequencing the complete genome. Using the set of SNP markers, it was possible to define the genetic relationships within a collection of forty-eight melon accessions as efficiently as with SSR markers, and these markers may also be useful for c...
Source: BMC Plant Biology - Latest articles - July 14, 2009 Category: Biology Authors: Wim DeleuCristina EsterasCristina RoigMireia Gonzalez-ToIria Fernandez-SilvaDaniel Gonzalez-IbeasJose BlancaMiguel ArandaPere ArusFernando NuezAntonio MonforteMaria Belen PicoJordi Garcia-Mas Source Type: journals
Tissue culture-induced transpositional activity of mPing is correlated with cytosine methylation in rice
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Conclusion:
Our results documented that tissue culture-induced mPing activity in rice ssp. indica is correlated with alteration in cytosine methylation patterns at both random genomic loci and the elements' flanks, while the stability of mPing positively correlates with enhanced methylation levels of both the flanks and probably the elements per se. Thus, our results implicate a possible role of cytosine methylation in maintaining mPing stability under normal conditions, and in releasing the element's activity as a consequence of epigenetic perturbation in a locus-specific manner under certain stress conditions. (Source: B...
Source: BMC Plant Biology - Latest articles - July 14, 2009 Category: Biology Authors: Frederic NgezahayoChunming XuHongyan WangLily JiangJinsong PangBao Liu Source Type: journals
Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissues
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Conclusions:
A large number of genes preferentially expressed in nectaries, as well as between nectary types and developmental stages, were identified. Several hypotheses relating to mechanisms of nectar production and regulation thereof are proposed, and provide a starting point for reverse genetics approaches to determine molecular mechanisms underlying nectar synthesis and secretion. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 14, 2009 Category: Biology Authors: Brian KramWayne XuClay Carter Source Type: journals
New sources of soybean seed meal and oil composition traits identified through TILLING
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Conclusions:
The oligosaccharide phenotype controlled by the novel RS2 allele is similar to previously observed seed oligosaccharide phenotypes in RS2 mutant (PI 200508) allele-containing lines. Due to the anti-nutritional characteristics of raffinose and stachyose, this represents a positive change in seed composition. The fatty acid phenotype controlled by the novel FAD2-1A allele controls an increase in oleic acid in the seed oil, a phenotype also observed in a line previously characterized to have a null allele of the FAD2-1A gene. Molecular marker assays were developed to reliably detect the inheritance of the mutant ...
Source: BMC Plant Biology - Latest articles - July 13, 2009 Category: Biology Authors: Emily DierkingKristin Bilyeu Source Type: journals
Development of SSR markers and analysis of diversity in Turkish populations of Brachypodium distachyon
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Conclusions:
Taken together, the inbreeding nature and genotypic diversity observed at individual locations suggest a significant amount of long-distance seed dispersal. The resources developed in this study are freely available to the research community and will facilitate experimental applications based on natural diversity. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 12, 2009 Category: Biology Authors: John VogelMetin TunaHikmet BudakNaxin HuoYong GuMichael Steinwand Source Type: journals
Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR
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Conclusions:
In summary, our results showed that some known miRNAs were expressed in Arabidopsis mature pollen, with most of them being low abundant. The results can be utilized in future research to study post-transcriptional gene regulation in pollen function. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 9, 2009 Category: Biology Authors: Carrie ChambersBin Shuai Source Type: journals
Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes
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Conclusions:
This study functionally identified sesquiterpene synthase genes predominantly expressed in sunflower trichomes. Evidence for the transcriptional regulation of sesquiterpene synthase genes in trichome cells suggest a potential use for these specialized cells for the identification of further genes involved in the biosynthesis, transport, and regulation of sesquiterpene lactones. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 5, 2009 Category: Biology Authors: Jens GopfertGilian MacNevinDae-Kyun RoOtmar Spring Source Type: journals
Sequence diversity in three tomato species: SNPs, markers, and molecular evolution
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Conclusions:
We present a new analysis of the extensive tomato EST sequences available that represents the most comprehensive survey of sequence diversity across Solanum species to date. These SNPs, plus thousands of molecular makers designed to detect the polymorphisms are available to the community via a website. Evolutionary analyses on these polymorphism uncovered sets of genes potentially important for the evolution and domestication of tomato; interestingly these sets were enriched for genes involved in response to the environment. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - July 2, 2009 Category: Biology Authors: Jose Jimenez-GomezJulin Maloof Source Type: journals
Selection of reference genes for quantitative real time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha
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Conclusions:
In total, eight genes belonging to different cellular processes were tested. Out of them, BbrizTUB was the less stable while BbrizEF1 followed by BbrizUBCE were the more stable genes considering male and female reproductive tissues, spikelets, roots and leaves. Regarding the best reference genes for ovary tissues, where apomictic and sexual reproduction must occur, the best reference genes were BbrizUBCE, BbrizE1F4A and BbrizEF1. Our results provide crucial information for transcriptional analysis in the Brachiaria ssp, helping to improve the quality of gene expression data in these species, which constitute a...
Source: BMC Plant Biology - Latest articles - July 1, 2009 Category: Biology Authors: Erica SilveiraMarcio Alves-FerreiraLarissa GuimaraesFelipe da SilvaVera Carneiro Source Type: journals
A membrane-bound matrix metalloproteinase from Nicotiana tabacum cv. BY-2 is induced by bacterial pathogens
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Conclusions:
Our biochemical analysis of NtMMP1 together with bioinformatic data on the primary sequence indicate that NtMMP1 is a constitutively-active protease. Given its induction in response to bacterial pathogens and its localization in the plasma membrane, we propose a role in pathogen defense at the cell periphery. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - June 28, 2009 Category: Biology Authors: Andreas SchiermeyerHanna HartensteinManoj MandalBurkhard OtteVerena WahnerStefan Schillberg Source Type: journals
Ontology-oriented retrieval of putative microRNAs in Vitis vinifera via GrapeMiRNA: a web database of de novo predicted grape microRNAs
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Conclusions:
The GrapeMiRNA database encompasses 5,778 microRNA predictions spanning the whole grape genome. Predictions are integrated with information that can be of use in selection procedures. Tools added in the web interface also allow to inspect predictions according to gene ontology classes and metabolic pathways of targets. The GrapeMiRNA database can be of help in selecting candidate microRNA genes to be validated. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - June 28, 2009 Category: Biology Authors: Barbara LazzariAndrea CapreraAlessandro CestaroIvan MerelliMarcello Del CorvoPaolo FontanaLuciano MilanesiRiccardo VelascoAlessandra Stella Source Type: journals
Phylogenetic analysis and molecular evolution of the dormancy associated MADS-box genes from peach
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Conclusions:
Because all DAM genes are expressed in peach and are subjected to strong purifying selection we suggest that the duplicated genes have been maintained by subfunctionalization and/or neofunctionalization. In addition, this pattern of selection suggests that the DAM genes are important for peach growth and development. (Source: BMC Plant Biology - Latest articles)
Source: BMC Plant Biology - Latest articles - June 26, 2009 Category: Biology Authors: Sergio JimenezAmy Lawton-RauhGregory ReighardAlbert AbbottDouglas Bielenberg Source Type: journals
