Comparison and optimization of protocols and whole-genome capture conditions for ancient DNA samples
Biotechniques. 2024 Mar 26. doi: 10.2144/btn-2023-0107. Online ahead of print.ABSTRACTAncient DNA (aDNA) obtained from human remains is typically fragmented and present in relatively low amounts. Here we investigate a set of optimal methods for producing aDNA data by comparing silica-based DNA extraction and aDNA library preparation protocols. We also test the efficiency of whole-genome enrichment (WGC) on ancient human samples by modifying a number of parameter combinations. We find that the Dabney extraction protocol performs significantly better than alternatives. We further observed a positive trend with the BEST libra...
Source: BioTechniques - March 26, 2024 Category: Biotechnology Authors: Reyhan Yaka None Maja Krzewi ńska Vendela Kempe Lagerholm Anna Linderholm F üsun Özer Mehmet Somel Anders G ötherström Source Type: research

Recording the brain < em > in vivo < /em > : emerging technologies for the exploration of mental health conditions
Biotechniques. 2024 Apr;76(4):121-124. doi: 10.2144/btn-2024-0013. Epub 2024 Mar 14.ABSTRACTStandfirst Mounting interest in mental health conditions over the last two decades has been coupled with the increasing sophistication of techniques to study the brain in vivo. [Formula: see text].PMID:38482795 | DOI:10.2144/btn-2024-0013 (Source: BioTechniques)
Source: BioTechniques - March 14, 2024 Category: Biotechnology Authors: Tristan Free Source Type: research

Recording the brain < em > in vivo < /em > : emerging technologies for the exploration of mental health conditions
Biotechniques. 2024 Mar 14. doi: 10.2144/btn-2024-0013. Online ahead of print.ABSTRACTStandfirst Mounting interest in mental health conditions over the last two decades has been coupled with the increasing sophistication of techniques to study the brain in vivo. [Formula: see text].PMID:38482795 | DOI:10.2144/btn-2024-0013 (Source: BioTechniques)
Source: BioTechniques - March 14, 2024 Category: Biotechnology Authors: Tristan Free Source Type: research

Recording the brain < em > in vivo < /em > : emerging technologies for the exploration of mental health conditions
Biotechniques. 2024 Mar 14. doi: 10.2144/btn-2024-0013. Online ahead of print.ABSTRACTStandfirst Mounting interest in mental health conditions over the last two decades has been coupled with the increasing sophistication of techniques to study the brain in vivo. [Formula: see text].PMID:38482795 | DOI:10.2144/btn-2024-0013 (Source: BioTechniques)
Source: BioTechniques - March 14, 2024 Category: Biotechnology Authors: Tristan Free Source Type: research

Recording the brain < em > in vivo < /em > : emerging technologies for the exploration of mental health conditions
Biotechniques. 2024 Mar 14. doi: 10.2144/btn-2024-0013. Online ahead of print.ABSTRACTStandfirst Mounting interest in mental health conditions over the last two decades has been coupled with the increasing sophistication of techniques to study the brain in vivo. [Formula: see text].PMID:38482795 | DOI:10.2144/btn-2024-0013 (Source: BioTechniques)
Source: BioTechniques - March 14, 2024 Category: Biotechnology Authors: Tristan Free Source Type: research

Recording the brain < em > in vivo < /em > : emerging technologies for the exploration of mental health conditions
Biotechniques. 2024 Mar 14. doi: 10.2144/btn-2024-0013. Online ahead of print.ABSTRACTStandfirst Mounting interest in mental health conditions over the last two decades has been coupled with the increasing sophistication of techniques to study the brain in vivo. [Formula: see text].PMID:38482795 | DOI:10.2144/btn-2024-0013 (Source: BioTechniques)
Source: BioTechniques - March 14, 2024 Category: Biotechnology Authors: Tristan Free Source Type: research

Recording the brain < em > in vivo < /em > : emerging technologies for the exploration of mental health conditions
Biotechniques. 2024 Mar 14. doi: 10.2144/btn-2024-0013. Online ahead of print.ABSTRACTStandfirst Mounting interest in mental health conditions over the last two decades has been coupled with the increasing sophistication of techniques to study the brain in vivo. [Formula: see text].PMID:38482795 | DOI:10.2144/btn-2024-0013 (Source: BioTechniques)
Source: BioTechniques - March 14, 2024 Category: Biotechnology Authors: Tristan Free Source Type: research

Recording the brain < em > in vivo < /em > : emerging technologies for the exploration of mental health conditions
Biotechniques. 2024 Mar 14. doi: 10.2144/btn-2024-0013. Online ahead of print.ABSTRACTStandfirst Mounting interest in mental health conditions over the last two decades has been coupled with the increasing sophistication of techniques to study the brain in vivo. [Formula: see text].PMID:38482795 | DOI:10.2144/btn-2024-0013 (Source: BioTechniques)
Source: BioTechniques - March 14, 2024 Category: Biotechnology Authors: Tristan Free Source Type: research

Recording the brain < em > in vivo < /em > : emerging technologies for the exploration of mental health conditions
Biotechniques. 2024 Mar 14. doi: 10.2144/btn-2024-0013. Online ahead of print.ABSTRACTStandfirst Mounting interest in mental health conditions over the last two decades has been coupled with the increasing sophistication of techniques to study the brain in vivo. [Formula: see text].PMID:38482795 | DOI:10.2144/btn-2024-0013 (Source: BioTechniques)
Source: BioTechniques - March 14, 2024 Category: Biotechnology Authors: Tristan Free Source Type: research

Recording the brain < em > in vivo < /em > : emerging technologies for the exploration of mental health conditions
Biotechniques. 2024 Mar 14. doi: 10.2144/btn-2024-0013. Online ahead of print.ABSTRACTStandfirst Mounting interest in mental health conditions over the last two decades has been coupled with the increasing sophistication of techniques to study the brain in vivo. [Formula: see text].PMID:38482795 | DOI:10.2144/btn-2024-0013 (Source: BioTechniques)
Source: BioTechniques - March 14, 2024 Category: Biotechnology Authors: Tristan Free Source Type: research

Isolation and characterization of < em > Paenibacillus peoriae < /em > JC-3jx from < em > Dendrobium nobile < /em >
In this study, endophytic bacteria from Dendrobium nobile were isolated using an improved method, showing inhibition of pathogens and growth promotion. JC-3jx, identified as Paenibacillus peoriae, exhibited significant inhibitory activity against tested fungi and bacteria, including Escherichia coli. JC-3jx also promoted corn seed rooting and Dendrobium growth, highlighting its excellent biocontrol and growth-promoting potential.PMID:38469872 | DOI:10.2144/btn-2023-0083 (Source: BioTechniques)
Source: BioTechniques - March 12, 2024 Category: Biotechnology Authors: ZhiPing Li Yuan Lin FeiFei Song RuoNan Zheng QinGeng Huang Source Type: research

Isolation and characterization of < em > Paenibacillus peoriae < /em > JC-3jx from < em > Dendrobium nobile < /em >
In this study, endophytic bacteria from Dendrobium nobile were isolated using an improved method, showing inhibition of pathogens and growth promotion. JC-3jx, identified as Paenibacillus peoriae, exhibited significant inhibitory activity against tested fungi and bacteria, including Escherichia coli. JC-3jx also promoted corn seed rooting and Dendrobium growth, highlighting its excellent biocontrol and growth-promoting potential.PMID:38469872 | DOI:10.2144/btn-2023-0083 (Source: BioTechniques)
Source: BioTechniques - March 12, 2024 Category: Biotechnology Authors: ZhiPing Li Yuan Lin FeiFei Song RuoNan Zheng QinGeng Huang Source Type: research

Isolation and characterization of < em > Paenibacillus peoriae < /em > JC-3jx from < em > Dendrobium nobile < /em >
In this study, endophytic bacteria from Dendrobium nobile were isolated using an improved method, showing inhibition of pathogens and growth promotion. JC-3jx, identified as Paenibacillus peoriae, exhibited significant inhibitory activity against tested fungi and bacteria, including Escherichia coli. JC-3jx also promoted corn seed rooting and Dendrobium growth, highlighting its excellent biocontrol and growth-promoting potential.PMID:38469872 | DOI:10.2144/btn-2023-0083 (Source: BioTechniques)
Source: BioTechniques - March 12, 2024 Category: Biotechnology Authors: ZhiPing Li Yuan Lin FeiFei Song RuoNan Zheng QinGeng Huang Source Type: research

A free and user-friendly software protocol for the quantification of microfauna swimming behavior
Biotechniques. 2024 Feb 29. doi: 10.2144/btn-2024-0001. Online ahead of print.ABSTRACTCharacterizing swimming behavior can provide a holistic assessment of the health, physiology and ecology of microfaunal species when done in conjunction with measuring other biological parameters. However, tracking and quantifying microfauna swimming behavior using existing automated tools is often difficult due to the animals' small size or transparency, or because of the high cost, expertise, or labor needed for the analysis. To address these issues, we created a cost-effective, user-friendly protocol for behavior analysis that employs ...
Source: BioTechniques - March 1, 2024 Category: Biotechnology Authors: Colleen M McMaken Kristin E Gribble Source Type: research

Multiparameter screen optimizes immunoprecipitation
Biotechniques. 2024 Feb 29. doi: 10.2144/btn-2023-0051. Online ahead of print.ABSTRACTImmunoprecipitation (IP) coupled with mass spectrometry effectively maps protein-protein interactions when genome-wide, affinity-tagged cell collections are used. Such studies have recorded significant portions of the compositions of physiological protein complexes, providing draft 'interactomes'; yet many constituents of protein complexes still remain uncharted. This gap exists partly because high-throughput approaches cannot optimize each IP. A key challenge for IP optimization is stabilizing in vivo interactions during the transfer fro...
Source: BioTechniques - March 1, 2024 Category: Biotechnology Authors: Shaoshuai Xie Leila Saba Hua Jiang Omar R Bringas Mehrnoosh Oghbaie Luciano Di Stefano Vadim Sherman John LaCava Source Type: research