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220 records returned

Genetic Relationships Within and Between Capsicum Species.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Genetic relationships were estimated among 24 accessions belonging to 11 species of Capsicum, using 2,760 RAPD markers based on touch-down polymerase chain reactions (Td-RAPD-PCR). These markers were implemented in analyses of principal coordinates, unweighted pair group mean average, and 2,000 bootstrap replications. The accessions were divided into four groups, corresponding to previously described Capsicum complexes: C. annuum complex (C(A)), C. baccatum complex (C(B)), C. pubescens complex (C(P)), and C. chacoense accessions (C(A)/(B)). Their overall mean genetic similarity index was 0.487 +/- 0.082, ranging from 0...
Source: Biochemical Genetics - November 15, 2009 Category: Genetics & Stem Cells Authors: Ince AG, Karaca M, Onus AN Tags: Biochem Genet Source Type: journals

A Decreasing Gradient of 374F Allele Frequencies in the Skin Pigmentation Gene SLC45A2, from the North of West Europe to North Africa.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the detailed distribution of the 374F allele has been investigated in 2063 unrelated subjects from 18 European and three North African populations. The highest allele frequency is observed in Denmark (0.980), and the lowest frequencies are observed in Tunisia (0.610) and in Morocco (0.691). A significant latitudinal cline in 374F allele frequencies was observed, ranging from the north of West Europe to North Africa (r = 0.869). The results confirm that the distribution of the 374F allele may reflect the ultraviolet radiation level and can be associated with skin color variation in these regions. PMID: 19...
Source: Biochemical Genetics - November 15, 2009 Category: Genetics & Stem Cells Authors: Lucotte G, Mercier G, Diéterlen F, Yuasa I Tags: Biochem Genet Source Type: journals

Necessity of Quantum Coherence to Account for the Spectrum of Time-Dependent Mutations Exhibited by Bacteriophage T4.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Transcriptase measurements of quantum expectations due to time-dependent coherent states populating informational DNA base-pair sites, designated by G-C --> *G-*C, G-C --> G'-C', and A-T --> *A-*T, provide a model for transcription and replication of time-dependent DNA lesions exhibited by bacteriophage T4. Coherent states are introduced as consequences of hydrogen bond arrangement, keto-amino --> enol-imine, where product protons are shared between two sets of indistinguishable electron lone-pairs and thus participate in coupled quantum oscillations at frequencies of ~10(13) s(-1). The transcriptase deciph...
Source: Biochemical Genetics - October 31, 2009 Category: Genetics & Stem Cells Authors: Cooper WG Tags: Biochem Genet Source Type: journals

Erratum to: Bioinformatics Analysis of Lactoferrin Gene for Several Species.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19856095 [PubMed - as supplied by publisher] (Source: Biochemical Genetics)
Source: Biochemical Genetics - October 24, 2009 Category: Genetics & Stem Cells Authors: Kang JF, Li XL, Zhou RY, Li LH, Feng FJ, Guo XL Tags: Biochem Genet Source Type: journals

Comparative Analysis of Genetic Diversity and Population Structure of Sipunculus nudus as Revealed by Mitochondrial COI Sequences.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Genetic diversity and population structure of Sipunculus nudus were evaluated using a 652 base pair fragment of the mitochondrial cytochrome oxidase I gene. The populations were collected from Beihai, Sanya, and Xiamen. A total of 71 polymorphic sites defined 16 distinct haplotypes. The mean haplotype diversity and nucleotide diversity of the three populations were 0.9354 +/- 0.0168 and 0.0035 +/- 0.0018, respectively. Analysis at the intrapopulation level showed that the Beihai population had the greatest haplotype and nucleotide diversity, followed by the Xiamen and Sanya populations. Analysis of molecular variance s...
Source: Biochemical Genetics - October 14, 2009 Category: Genetics & Stem Cells Authors: Du X, Chen Z, Deng Y, Wang Q Tags: Biochem Genet Source Type: journals

Genetic Diversity Assessment of Trollius Accessions in China by RAPD Markers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19809874 [PubMed - as supplied by publisher] (Source: Biochemical Genetics)
Source: Biochemical Genetics - October 6, 2009 Category: Genetics & Stem Cells Authors: Li Y, Ding WL Tags: Biochem Genet Source Type: journals

Prion Protein Polymorphisms and Estimation of Risk of Scrapie in East Asian Sheep.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Allele and genotype frequency distributions of prion protein (PrP) polymorphisms at three codons, 136, 154, and 171, in East Asian sheep were determined by PCR-RFLP analysis using 553 animals from nine local breeds of the northern group and four local breeds of the southern group. Based on the genotype distribution, the risk score for scrapie was estimated. Among the local breeds, ARQ appeared predominantly (0.7701-1), followed by ARH and ARR. From such a biased allele distribution, it was difficult to ascertain the prevalent genetic relationships. A marked difference in allele frequencies between the northern and sout...
Source: Biochemical Genetics - September 2, 2009 Category: Genetics & Stem Cells Authors: Tsunoda K, Namikawa T, Sato K, Hasnath MA, Nyunt MM, Rajbandary HB, Loc CB, Zanchiv T, Chang H, Sun W, Dorji T Tags: Biochem Genet Source Type: journals

Phylogeny of Local Sheep Breeds in East Asia, Focusing on the Bayanbulak Sheep in China and the Sipsu Sheep in Bhutan.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The phylogenetic positions of the Bayanbulak sheep in China and the Sipsu sheep in Bhutan in the northern Asian sheep group were determined on the basis of allele frequency data for five informative and polymorphic loci of blood protein and nonproteins, such as transferrin (TF), arylesterase (ES), hemoglobin-beta (HB-beta), X-protein (XP), and potassium transport (KE), using different electrophoretic and ion-densitometric techniques. Based on Nei's genetic distance, clustering analysis by the UPGMA method showed that the Bayanbulak sheep is clustered in the northern Asian sheep group. Furthermore, the Bayanbulak sheep ...
Source: Biochemical Genetics - September 2, 2009 Category: Genetics & Stem Cells Authors: Tsunoda K, Chang H, Chang G, Sun W, Dorji T, Tshering G, Yamamoto Y, Namikawa T Tags: Biochem Genet Source Type: journals

Noninvasive Genotyping of the Red-Legged Partridge (Alectoris rufa, Phasianidae): Semi-Nested PCR of Mitochondrial DNA from Feces.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
DNA-based studies using avian feces are scarce and deal only with large-sized species. The red-legged partridge, Alectoris rufa, is a medium-sized member of the order Galliformes. Our goal was to set up a fast, noninvasive procedure for mitochondrial DNA (mtDNA) genotyping of A. rufa fecal samples. We focused on the protected population from Elba Island (Tuscan Archipelago National Park, Italy). Dry A. rufa fecal samples (n = 30) were collected in winter. Both the cytochrome b gene (1,092 bp) and the control region (ca. 1,155 bp) were amplified by means of semi-nested PCRs. Twenty-five samples were successfully sequenc...
Source: Biochemical Genetics - August 27, 2009 Category: Genetics & Stem Cells Authors: Guerrini M, Barbanera F Tags: Biochem Genet Source Type: journals

Biochemical and Molecular Characterization of Some Centaurea Species Growing in the Eastern Anatolia Region of Turkey.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Random amplified polymorphic DNA (RAPD) and fatty acid (FAME) profiles were used to examine phenotypic and genetic relationships among 16 Centaurea species growing wild in the eastern Anatolia region of Turkey. Thirteen decamer primers were used to examine polymorphism. According to the RAPD results, 99 amplicons in the size range of 50-1000 bp were produced from 13 primers in 16 Centaurea species. Genetically four distinct groups were determined among the species of Centaurea, which represents high genetic variation. In the 16 species, 14 fatty acids were determined according to FAME results. Both FAME and RAPD result...
Source: Biochemical Genetics - August 11, 2009 Category: Genetics & Stem Cells Authors: Yildirim N, Sunar S, Agar G, Bozari S, Aksakal O Tags: Biochem Genet Source Type: journals

Two Sympatric Phylogroups of the Chinese Water Deer (Hydropotes inermis) Identified by Mitochondrial DNA Control Region and Cytochrome b Gene Analyses.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mitochondrial DNA (mtDNA) control region (927 bp) and cytochrome b gene (1,140 bp) sequences of the Chinese water deer (Hydropotes inermis) from China and Korea were obtained to examine the taxonomic status of two subspecies, H. i. inermis from China and H. i. argyropus from Korea. Two sympatric mtDNA clades (a major clade from China and Korea and a minor clade from Korea) with an average genetic distance of 2.1% in the control region and 1.3% in the cytochrome b gene were detected. These findings are not consistent with the current classification by pelage color. We propose a reconsideration of the validity of the sub...
Source: Biochemical Genetics - August 10, 2009 Category: Genetics & Stem Cells Authors: Koh HS, Lee BK, Wang J, Heo SW, Jang KH Tags: Biochem Genet Source Type: journals

Recurring G12S Mutation of HRAS in a Chinese Child with Costello Syndrome with High Alkaline Phosphatase Level.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19669404 [PubMed - as supplied by publisher] (Source: Biochemical Genetics)
Source: Biochemical Genetics - August 7, 2009 Category: Genetics & Stem Cells Authors: Zhang H, Ye J, Gu X Tags: Biochem Genet Source Type: journals

EHGbox in the Clitellate Perionyx excavatus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19669405 [PubMed - as supplied by publisher] (Source: Biochemical Genetics)
Source: Biochemical Genetics - August 7, 2009 Category: Genetics & Stem Cells Authors: Cho SJ, Park SC Tags: Biochem Genet Source Type: journals

Contrary Effects of BMP-2 and ATRA on Adipogenesis in Mouse Mesenchymal Fibroblasts.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study evaluates the effects of bone morphogenetic protein 2 (BMP-2) and all trans retinoic acid (ATRA) on adipogenesis in primary mouse embryo fibroblasts (MEFs). In BMP-2-treated MEFs, lipid accumulation and substantial induction of the adipocyte specific marker 442-aP2 suggested the conversion of MEFs into adipocytes. Such adipogenesis was found to be mediated through sequential induction of C/EBPalpha, C/EBPbeta, and PPARgamma. Both the BMP/Smad and BMP/p38 pathways contributed to the adipocyte differentiation. Contrary to the effects of BMP-2, ATRA was demonstrated to inhibit adipocyte differentiation in MEFs. Sem...
Source: Biochemical Genetics - August 7, 2009 Category: Genetics & Stem Cells Authors: Sun F, Pan Q, Wang J, Liu S, Li Z, Yu Y Tags: Biochem Genet Source Type: journals

Genetic Variability and Geographic Differentiation in Thymus daenensis subsp. daenensis, an Endangered Medicinal Plant, as Revealed by Inter Simple Sequence Repeat (ISSR) Markers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Thymus daenensis is an aromatic medicinal plant endemic to Iran. We used inter simple sequence repeat (ISSR) markers to detect genetic polymorphism in this herb using 17 T. daenensis accessions collected from different geographic regions in Iran. The 15 primers chosen for analysis revealed 256 bands, of which 228 (88.9%) were polymorphic. Jaccard's similarity indices based on ISSR profiles were subjected to UPGMA cluster analysis. The generated dendrogram revealed two major groups. The Tc group included the accessions collected from the center of the Zagros Mountains, and the Te group was collected from the extremes of...
Source: Biochemical Genetics - August 5, 2009 Category: Genetics & Stem Cells Authors: Rahimmalek M, Bahreininejad B, Khorrami M, Sayed Tabatabaei BE Tags: Biochem Genet Source Type: journals

Expression Pattern and Prokaryotic Expression for Peking Duck Insulin-Like Growth Factor-I in Escherichia coli.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study investigated its expression pattern in various tissues in Peking duck and in vivo. The result showed that the recombinant protein of IGF-I was expressed mainly in the form of inclusion bodies in the Escherichia coli cell (37.4 kD). Western blotting indicated that the recombinant protein had the antigenicity of IGF-I. Reverse-transcript PCR and real-time fluorescent quantitative PCR analyses showed that IGF-I was expressed in the heart, liver, abdominal fat, breast muscle, spleen, lung, kidney, gizzard, and small intestine of 8-week-old birds. In the RT-PCR results, IGF-I was expressed highest in the heart and lu...
Source: Biochemical Genetics - July 28, 2009 Category: Genetics & Stem Cells Authors: Wu Y, Liu XL, Xiao HW, Wang J, Jiang F, Zhao S, Liu Y Tags: Biochem Genet Source Type: journals

Molecular Characterization, Mapping, and Haplotype Analysis of Porcine Matrix Metalloproteinase Genes MMP1 and MMP10.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report the identification of two members of the MMP gene family in swine. The porcine MMP1 and MMP10 genes comprise 10 exons and 9 introns spanning approximately 8,460 and 7,030 bp. Of 28 potential single nucleotide polymorphisms found in the genomic region, five polymorphic positions were analyzed using PCR-RFLP. Allelic frequencies and haplotypes were analyzed in five pig breeds (n = 280). The AC haplotype of MMP1 and ATG haplotype of MMP10 were not detected in two foreign pig breeds. Association analysis in a French Large White population (n = 164, total four traits) showed no association between haplotypes and repro...
Source: Biochemical Genetics - July 28, 2009 Category: Genetics & Stem Cells Authors: Wu X, Pan Y Tags: Biochem Genet Source Type: journals

Organization of a beta and alpha Globin Gene Set in the Teleost Atlantic Cod, Gadus morhua.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Developmental globin gene expression and gene switching in vertebrates have been extensively studied. Globin gene regions have been characterized in some fish species and show linked alpha and beta loci. Understanding coordinated expression between alpha and beta globin genes in fish is of importance for further insights into globin gene regulation in teleosts and higher vertebrates. We characterize linked beta and alpha globin genes in Atlantic cod, pulled from the Atlantic cod genome with a PCR research strategy, by screening a genomic lambda library and primer walking. The genes are oriented tail-to-head (5'-3'), di...
Source: Biochemical Genetics - July 25, 2009 Category: Genetics & Stem Cells Authors: Halldórsdóttir K, Arnason E Tags: Biochem Genet Source Type: journals

Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated b...
Source: Biochemical Genetics - July 25, 2009 Category: Genetics & Stem Cells Authors: Wu N, Li CJ, Gui JF Tags: Biochem Genet Source Type: journals

Comparative Analyses of Disease Risk Genes Belonging to the Acyl-CoA Synthetase Medium-Chain (ACSM) Family in Human Liver and Cell Lines.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The human ACSM1, 2A and B, 3, and 5 genes, located on chromosome 16p12-13, encode for enzymes catalyzing the activation of medium-chain length fatty acids. Association studies have linked several polymorphisms of these genes to traits of insulin resistance syndrome. In our study, ACSM transcripts showed 3 to >400-fold higher expression levels in human liver when compared to cell lines by qRT-PCR. This difference was also evident at the protein level, as shown for ACSM2. In liver, ACSM2 was the most abundant transcript, showing sixfold (vs. ACSM3) to >300-fold higher expression levels (vs. ACSM1). Mitochondrial lo...
Source: Biochemical Genetics - July 25, 2009 Category: Genetics & Stem Cells Authors: Boomgaarden I, Vock C, Klapper M, Döring F Tags: Biochem Genet Source Type: journals

An Exonic Insertion Encodes an Alanine Stretch in Porcine Synapsin I.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19633946 [PubMed - as supplied by publisher] (Source: Biochemical Genetics)
Source: Biochemical Genetics - July 24, 2009 Category: Genetics & Stem Cells Authors: Hedegaard C, Bendixen E, Jensen PH, Bendixen C, Larsen K Tags: Biochem Genet Source Type: journals

Two Different Size Classes of 5S rDNA Units Coexisting in the Same Tandem Array in the Razor Clam Ensis macha: Is This Region Suitable for Phylogeographic Studies?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
For a study of 5S ribosomal genes (rDNA) in the razor clam Ensis macha, the 5S rDNA region was amplified and sequenced. Two variants, so-called type I or short repeat (~430 bp) and type II or long repeat (~735 bp), appeared to be the main components of the 5S rDNA of this species. Their spacers differed markedly, both in length and nucleotide composition. The organization of the two variants was investigated by amplifying the genomic DNA with primers based on the sequence of the type I and type II spacers. PCR amplification products with primers EMLbF and EMSbR showed that the long and short repeats are associated with...
Source: Biochemical Genetics - July 24, 2009 Category: Genetics & Stem Cells Authors: Fernández-Tajes J, Méndez J Tags: Biochem Genet Source Type: journals

Long-Term Evolution of 5S Ribosomal DNA Seems to Be Driven by Birth-and-Death Processes and Selection in Ensis Razor Shells (Mollusca: Bivalvia).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A study of nucleotide sequence variation of 5S ribosomal DNA from six Ensis species revealed that several 5S ribosomal DNA variants, based on differences in their nontranscribed spacers (NTS), occur in Ensis genomes. The 5S rRNA gene was not very polymorphic, compared with the NTS region. The phylogenetic analyses performed showed a between-species clustering of 5S ribosomal DNA variants. Sequence divergence levels between variants were very large, revealing a lack of sequence homogenization. These results strongly suggest that the long-term evolution of Ensis 5S ribosomal DNA is driven by birth-and-death processes and...
Source: Biochemical Genetics - July 24, 2009 Category: Genetics & Stem Cells Authors: Vierna J, González-Tizón AM, Martínez-Lage A Tags: Biochem Genet Source Type: journals

Development of Microsatellite Markers from an Enriched Genomic Library for the Genetic Analysis of the Malaysian Giant Freshwater Prawn, Macrobrachium rosenbergii.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19603264 [PubMed - as supplied by publisher] (Source: Biochemical Genetics)
Source: Biochemical Genetics - July 14, 2009 Category: Genetics & Stem Cells Authors: Min See L, Tan SG, Hassan R, Siraj SS, Bhassu S Tags: Biochem Genet Source Type: journals

Allele Frequency Distribution Data for D8S1132, D8S1779, D8S514, and D8S1743 in Four Ethnic Groups in Relation to Metabolic Syndrome: Tehran Lipid and Glucose Study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19597983 [PubMed - as supplied by publisher] (Source: Biochemical Genetics)
Source: Biochemical Genetics - July 13, 2009 Category: Genetics & Stem Cells Authors: Daneshpour MS, Alfadhli S, Houshmand M, Zeinali S, Hedayati M, Zarkesh M, Momenan AA, Azizi F Tags: Biochem Genet Source Type: journals

MLPA Subtelomere Analysis in Tunisian Mentally Retarded Patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Subtelomeric rearrangements significantly contribute to idiopathic mental retardation and result in several mental retardation syndromes; however, most subtelomeric defects lack a characteristic phenotype. Thirty patients with unexplained mental retardation, a normal R banded karyotype at the 550 band, and no clinically recognizable syndrome were screened by Multiplex ligation-dependent probe amplification (MLPA). Four anomalies were identified: deletion 17q, duplications (4q), and associated duplications 15q and Xq. This duplication was found in two sisters of the proband. Anomalies were unidentified by the convention...
Source: Biochemical Genetics - July 9, 2009 Category: Genetics & Stem Cells Authors: Hila L, Tébourbi H, Abaied L, Rejeb I, Ben Jemaa L, Chaabouni H Tags: Biochem Genet Source Type: journals

Low Genetic Variability of Domestic Muscovy Duck (Cairina moschata) in China Revealed by Mitochondrial DNA Control Region Sequences.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19588244 [PubMed - as supplied by publisher] (Source: Biochemical Genetics)
Source: Biochemical Genetics - July 8, 2009 Category: Genetics & Stem Cells Authors: Chen SY, He DQ, Liu YP Tags: Biochem Genet Source Type: journals

Tumor Necrosis Factor Alpha and Interleukin 10 Promoter Polymorphisms in Mexican Patients with Restenosis After Coronary Stenting.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To test for an association with risk for restenosis after coronary stent placement, the TNF-alpha and IL-10 polymorphisms were analyzed by 5' exonuclease TaqMan assays in 162 patients who initially underwent coronary stenting. Analysis of basal and procedure coronary angiographies revealed a higher proportion of restenosis in lesions treated with bare metal stents compared with those treated with drug-eluting stents (P < 0.001). Distribution of TNF-alpha genotypes was similar in patients with and without restenosis. The IL-10 polymorphisms showed a moderate protective trend of the -819 TT genotype against restenosis...
Source: Biochemical Genetics - July 7, 2009 Category: Genetics & Stem Cells Authors: Martínez-Ríos MA, Peña-Duque MA, Fragoso JM, Delgadillo-Rodríguez H, Rodríguez-Pérez JM, Miranda-Malpica E, Cruz-Robles D, Cavazos-Quero MM, Rodríguez-Lobato LG, Vargas-Alarcón G Tags: Biochem Genet Source Type: journals

Mitochondrial DNA Variation in the CoxI-CoxII Intergenic Region among Turkish and Iranian Honey Bees (Apis mellifera L.).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19579064 [PubMed - as supplied by publisher] (Source: Biochemical Genetics)
Source: Biochemical Genetics - July 4, 2009 Category: Genetics & Stem Cells Authors: Ozdil F, Fakhri B, Meydan H, Yıldız MA, Hall HG Tags: Biochem Genet Source Type: journals

A Genomic Insight into Diversity Among Tribal and Nontribal Population Groups of Manipur, India.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Twenty autosomal markers, including linked markers at two gene markers, are used to understand the genomic similarity and diversity among three tribal (Paite, Thadou, and Kom) and one nontribal communities of Manipur (Northeast India). Two of the markers (CD4 and HB9) are monomorphic in Paite and one (the CD4 marker) in Kom. Data suggest the Meitei (nontribal groups) stand apart from the three tribal groups with respect to higher heterozygosity (0.366) and presence of the highest ancestor haplotypes of DRD2 markers (0.228); this is also supported by principal co-ordinate analysis. These populations are found to be geno...
Source: Biochemical Genetics - June 29, 2009 Category: Genetics & Stem Cells Authors: Saraswathy KN, Kiranmala N, Murry B, Sinha E, Saksena D, Kaur H, Sachdeva MP, Kalla AK Tags: Biochem Genet Source Type: journals

Apolipoprotein E Polymorphism in Hemodialyzed Patients and Healthy Controls.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study suggests that the APOE2 allele is a possible genetic risk factor for all-cause ESRD in Caucasians. PMID: 19565203 [PubMed - as supplied by publisher] (Source: Biochemical Genetics)
Source: Biochemical Genetics - June 29, 2009 Category: Genetics & Stem Cells Authors: Hubacek JA, Bloudickova S, Kubinova R, Pikhart H, Viklicky O, Bobak M Tags: Biochem Genet Source Type: journals

Genetic Stability of Progeny from an Artificial Allotetraploid Carp Using Sperm from Five Fish Species.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Microsatellite markers and D-loop sequences of mtDNA from a female allotetraploid parent carp and her progenies of generations 1 and 2 induced by sperm of five distant fish species were analyzed. Eleven microsatellite markers were used to identify 48 alleles from the allotetraploid female. The same number of alleles (48) appeared in the first and second generations of the gynogenetic offspring, regardless of the source of the sperm used as an activator. The mtDNA D-loop analysis was performed on the female tetraploid parent, 25 gynogenetic offspring, and 5 sperm-donor species. Fourteen variable sites from the 1,018 bp ...
Source: Biochemical Genetics - June 29, 2009 Category: Genetics & Stem Cells Authors: Ye Y, Wang Z, Zhou J, Wu Q Tags: Biochem Genet Source Type: journals

STR Polymorphisms of the Henan Population and Investigation of the Central Plains Han Origin of Chaoshanese.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Allele frequencies for 15 short tandem repeat (STR) loci were obtained from a Chinese Han population in Henan province of middle China. No deviation from Hardy-Weinberg equilibrium was observed for the STR loci except for D3S1358. The 15 STR loci are potentially useful for paternity testing and forensic casework in the Henan population. A phylogenetic tree based on CODIS STR allele frequencies of 25 Han populations revealed noticeable but far less clear distinctions between southern and northern Chinese populations; the Henan Han population was located at an intermediate position between south and north Chinese Han pop...
Source: Biochemical Genetics - June 23, 2009 Category: Genetics & Stem Cells Authors: Xu LN, Hu SP, Feng GY Tags: Biochem Genet Source Type: journals

Molecular Evolution of TEPP Protein Genes in Metazoans.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
TEPP is a gene expressed in human reproductive organs such as testis, prostate, and placenta. Here, identification and molecular evolutionary analysis of TEPP proteins in various metazoan animals including deuterostomes (chordates, hemichordates, and echinoderms), lophotrochozoans (mollusks and annelids), and cnidarians (sea anemone and coral) are reported. A multiple sequence alignment revealed two highly conserved regions in TEPP proteins that had no similarity to any other known domains or proteins. Genomic sequence analysis showed frequent shifting of the splice sites of intron 1 in mammalian TEPP genes. A comparis...
Source: Biochemical Genetics - June 22, 2009 Category: Genetics & Stem Cells Authors: Hahn Y Tags: Biochem Genet Source Type: journals

Genetic Diversity and Genetic Structure Analysis of the Natural Populations of Lilium brownii from Guangdong, China.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19543969 [PubMed - as supplied by publisher] (Source: Biochemical Genetics)
Source: Biochemical Genetics - June 20, 2009 Category: Genetics & Stem Cells Authors: Huang YF, Yang MX, Zhang H, Zhuang XY, Wu XH, Xie W Tags: Biochem Genet Source Type: journals

Analysis of STAT5A/AvaI Gene Polymorphism in Four Italian Cattle Breeds.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The STAT5A/AvaI polymorphism was investigated with PCR-RFLP in a sample of 339 cattle belonging to four breeds: Italian Friesian, Jersey, Italian Brown, and Podolica reared in south Italy. All three possible genotypes for the C/T polymorphism were identified. In these breeds, PCR-RFLP showed the predominance of the TT genotype in Italian Brown and Jersey cows; in Podolica and Italian Friesian CT is the most frequent genotype. The frequency of the T allele ranged from 0.55 to 0.81 in the analyzed populations. The distribution of genotypic and allelic frequencies at this locus was significantly different among the four p...
Source: Biochemical Genetics - June 20, 2009 Category: Genetics & Stem Cells Authors: Dario C, Dario M, Ciotola F, Peretti V, Carnicella D, Selvaggi M Tags: Biochem Genet Source Type: journals

A 2.7-kb Deletion in the Human NLRP10 Gene Exon 2 Occurred After the Human-Chimpanzee Divergence.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
NLRP10 is a member of the NLRP protein family, which is involved in inflammation and apoptosis. Genome sequence comparisons revealed that a 2.7-kb deletion occurred in the human NLRP10 gene exon 2 after the divergence of humans and chimpanzees, resulting in replacement of the entire 3' untranslated region with the flanking LINE-1 element. The human NLRP10 protein lost 30 or more amino acids that are conserved in primates at its carboxy-terminus. The structural modification of the NLRP10 gene might have played a role in development or enhancement of human-specific traits during evolution. PMID: 19544093 [PubMed - as...
Source: Biochemical Genetics - June 20, 2009 Category: Genetics & Stem Cells Authors: Ha HJ, Kim DS, Hahn Y Tags: Biochem Genet Source Type: journals

Genetic Diversity and Population Structure of Celosia argentea and Related Species Revealed by SRAP.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Genetic diversity of 16 populations of Celosia argentea L. and 6 populations of Celosia cristata L. in China was investigated using sequence-related amplified polymorphism (SRAP). Ten SRAP primer combinations generated 507 scorable amplification bands ranging from 50 to 2000 bp, among which 274 were polymorphic, with an average of 54 polymorphic bands per primer combination. The unweighted pair group method of arithmetic averages (UPGMA) cluster analysis enabled construction of a phylogenetic tree for estimating genetic distance among populations, which agreed well with the geographic origin information. Twenty-two pop...
Source: Biochemical Genetics - June 11, 2009 Category: Genetics & Stem Cells Authors: Feng N, Xue Q, Guo Q, Zhao R, Guo M Tags: Biochem Genet Source Type: journals

MTHFR Polymorphisms Involved in Vitamin B(12) Deficiency Associated with Atrophic Gastritis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Genetic polymorphisms affecting methylentetrahydrofolate reductase (MTHFR) activity may influence hematological and neurological dysfunction in cobalamin-deficient patients. We studied the prevalence of C677T and A1298C polymorphisms by analyzing genomic DNA in 30 cobalamin-deficient patients. No significant difference was found in 677 and 1298 genotype distribution with respect to hematological parameters, B(12) and folate levels, and neurological symptoms. The two MTHFR polymorphisms were not protective against anemia or neurological dysfunction in patients with cobalamin deficiency; however, we found evidence of a s...
Source: Biochemical Genetics - June 11, 2009 Category: Genetics & Stem Cells Authors: Palladino M, Chiusolo P, Reddiconto G, Marietti S, De Ritis D, Leone G, Sica S Tags: Biochem Genet Source Type: journals

Over-Seasonal and Sex-Independent Expression of Kappa Casein Gene (CSN3) in Mammalian Blood Lymphocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
After observing the nonspecific expression of the alpha S1 casein gene in peripheral blood mononuclear cells of goats, we continued to trace illegitimate gene-expression patterns in mammals. Six mammalian species (Bos taurus, Capra hircus, Ovis aries, Equus caballus, Sus scrofa, and Bison bonasus) were analyzed for the presence of kappa casein in peripheral blood mononuclear cells and Ficoll-isolated lymphocytes. To detect potential relationships between the expression pattern and reproductive status of the individual, the animals within species represented different sex, age, and physiological status (immature and rep...
Source: Biochemical Genetics - June 9, 2009 Category: Genetics & Stem Cells Authors: Tokarska M, Ruczyńska I, Zalewska H, Wójcik AM Tags: Biochem Genet Source Type: journals

Genetic Polymorphisms of Cytochrome P450 and Matrix Metalloproteinase in Chronic Obstructive Pulmonary Disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We investigated whether polymorphisms of cytochrome P450 (CYP) and matrix metalloproteinase (MMP) are associated with the development and severity of chronic obstructive pulmonary disease (COPD). Genotypes of 184 patients with COPD and 212 controls were determined by polymerase chain reaction, followed by restriction fragment length polymorphism. The homozygous T allele of MMP-9 was significantly higher in patients with COPD than in controls (14.7% vs. 7.5%). The odds ratio was 2.4 (95% CI 1.1-5.9). No differences were observed in the frequency of polymorphic genotypes in CYP1A1, 1A2, MMP-1 and -3. During combined anal...
Source: Biochemical Genetics - June 8, 2009 Category: Genetics & Stem Cells Authors: Cheng SL, Yu CJ, Yang PC Tags: Biochem Genet Source Type: journals

Cloning and Expression of a Novel Chitinase chi58 from Chaetomium cupreum in Pichia pastoris.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A gene encoding a novel chitinase chi58 was cloned from the fungus Chaetomium cupreum by using inverse PCR. The DNA sequence of chi58 contains a 1,602 bp open reading frame and two introns that are 52 and 201 bp in length. Regarding our in silico analysis, chi58 is a modular enzyme composed of a family-18 catalytic domain, which is responsible for chitinase activity, and a chitin-binding domain containing several cysteines. Apparently, the function of these domains is to anchor the enzyme tightly onto the large insoluble polymeric substrate. Chi58 has a pI of 4.47 and a deduced molecular mass of 58 kDa. The optimal pH ...
Source: Biochemical Genetics - June 8, 2009 Category: Genetics & Stem Cells Authors: Wang YJ, Yang Q Tags: Biochem Genet Source Type: journals

Extensive Diversity of the ADRB3 Gene in Chinese Sheep Identified by PCR-SSCP.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we employed PCR-SSCP to detect ADRB3 polymorphism in the Hu, Tan, small-tailed Han, and Tibet breeds of Chinese sheep. Thirteen SSCP patterns were observed, including the eight previously reported and five new patterns. The newly identified SSCP patterns represent five novel sequences, increasing the reported number of alleles of ADRB3 from 8 to 13. In these Chinese sheep, the most common allele was the previously reported allele C (49.0%); the A allele was the next most common (22.0%). These results suggest that Chinese sheep have more diversity in ADRB3 than New Zealand sheep and therefore have possibly be...
Source: Biochemical Genetics - June 8, 2009 Category: Genetics & Stem Cells Authors: Yang G, Zhou H, Hu J, Luo Y, Hickford JG Tags: Biochem Genet Source Type: journals

Allele Frequency of VNTR Locus D1S80 Observed in Denizli Province of Turkey.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The variable numbers of tandem repeats (VNTR) locus D1S80, located on chromosome 1 (1p35-36), has a repeat unit 16 bp in length, and different numbers of these repeat units have been observed for populations of different origins and ethnicity. We used a molecular identification method based on capillary electrophoresis separation to analyze D1S80 locus polymorphism among 74 subjects from Denizli province, Turkey, finding an amplified fragment length size of 379-635 bp. Allele repeat numbers were deduced from these sizes and sequence comparison. The most common alleles were repeat units 24 (34.3%) and 18 (22.4%), with f...
Source: Biochemical Genetics - June 6, 2009 Category: Genetics & Stem Cells Authors: Köseler A, Atalay A, Atalay EO Tags: Biochem Genet Source Type: journals

Relationships Between Single Nucleotide Polymorphisms of the H-FABP Gene and Slaughter and Meat Quality Traits in Chicken.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Using PCR-SSCP with five primer pairs, we detected six single nucleotide polymorphisms of the H-FABP gene: 332G --> A, 534G --> A, 783C --> T, 835C --> T, 1198T --> C, and 2329C --> T. Chi-square results showed significant differences (P < 0.05) in genotype frequency among breeds in Fragment 1 and extremely significant differences (P < 0.01) in Fragments 2-4. We found a significant association between Fragment 2 genotype and muscle fiber number, Arg and Thr (P < 0.05); between Fragment 3 genotype and living weight, carcass weight, breast muscle weight, abdominal fat weight, and abdominal fat ...
Source: Biochemical Genetics - June 6, 2009 Category: Genetics & Stem Cells Authors: You X, Liu Y, Jiang X, Du H, Liu Z, Zhu Q Tags: Biochem Genet Source Type: journals

Association of the Hypoxia Inducible Factor-1alpha Gene Polymorphisms with Gastric Cancer in Tibetans.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To determine how single nucleotide polymorphisms (SNPs) in the hypoxia inducible factor-1alpha (HIF-1alpha) gene coding regions affect gastric cancer, the authors conducted an association study of the HIF-1alpha polymorphisms C1772T and G1790A for a Tibet population. DNA was extracted from peripheral blood of 87 gastric cancer patients and 106 controls and analyzed using the polymerase chain reaction/ligase detection reaction test for HIF-1alpha polymorphisms. There was a significant increase in the frequency of the GA 1790 genotype in patients with gastric cancer compared with healthy controls (OR 2.93; 95% CI 1.06-8....
Source: Biochemical Genetics - June 6, 2009 Category: Genetics & Stem Cells Authors: Li K, Zhang Y, Dan Z, Wang Y, Ren ZC Tags: Biochem Genet Source Type: journals

Angiotensinogen T174M and M235T variants and hypertension in the Hani and Yi minority groups of China.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A case-control study of 538 individuals investigated whether the angiotensinogen gene (AGT) might be implicated in the pathogenesis of essential hypertension in the Hani and Yi populations of China. Genotypes for two diallelic DNA polymorphisms observed at amino acid residues 174 (T174M) and 235 (M235T) within the coding sequence were determined. M235T and T174M genotyping with PCR-RFLP was performed in 267 normotensive subjects and 271 hypertensive subjects. No significant difference was found between normotensives and hypertensives in genotype distribution and allele frequency for either M235T or T174M in the Hani or...
Source: Biochemical Genetics - May 31, 2009 Category: Genetics & Stem Cells Authors: Yuan J, Tang W, Chun Y, Ying H, Yang Y, Xiao C Tags: Biochem Genet Source Type: journals

Prevalence of the angiotensin I converting enzyme gene insertion/deletion polymorphism in a healthy Turkish population.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study demonstrates that the allele and genotype frequency values for the Turkish population are similar to previously published frequencies for Caucasian populations. PMID: 19390959 [PubMed - in process] (Source: Biochemical Genetics)
Source: Biochemical Genetics - May 31, 2009 Category: Genetics & Stem Cells Authors: Berdeli A, Cam FS Tags: Biochem Genet Source Type: journals

Molecular characterization of the pina gene in einkorn wheat.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Fifty-six sequences encoding the pina protein were characterized from three species or subspecies of einkorn wheat. These sequences contained 1,595 nucleotides, including 1,270 conserved sites, 21 single nucleotide polymorphisms (SNPs), and 16 indels. The average frequency of SNPs and indels was one out of 76.1 and 99.9 bases, respectively. Five SNPs and no indels were found in the translated sequences. Fourteen haplotypes were defined, and the accessions in each haplotype ranged from 1 to 18. There were nine haplotypes in Triticum monococcum ssp. aegilopoides, eight in T. monococcum ssp. monococcum, and two in T. urar...
Source: Biochemical Genetics - May 31, 2009 Category: Genetics & Stem Cells Authors: Chen Q, Qi PF, Wei YM, Wang JR, Zheng YL Tags: Biochem Genet Source Type: journals

Genetic diversity in Metapenaeus dobsoni using RAPD analysis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19396627 [PubMed - in process] (Source: Biochemical Genetics)
Source: Biochemical Genetics - May 31, 2009 Category: Genetics & Stem Cells Authors: Mishra PS, Chaudhari A, Krishna G, Kumar D, Lakra WS Tags: Biochem Genet Source Type: journals