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The dsRNA isolated from Escherichia coli infected with the MS2 bacteriophage induces the production of interferons
This study commenced with the cultivation of host bacterium 15,597, followed by the infection of Escherichia coli with the MS2 bacteriophage. Utilizing the J2 capture technique, a class of dsRNA mixtures (MS2+15,597) was isolated from the E. coli infected with the MS2 bacteriophage. Subsequent investigations were conducted on the immunostimulatory activity of the MS2+15,597 mixture. The results indicated that the dsRNA mixtures (MS2+15,597) extracted from E. coli infected with the MS2 bacteriophage possess the capability to activate innate immunity, thereby inducing the production of interferon-β. These dsRNA mixtures can...
Source: Biochemical and Biophysical Research communications - April 25, 2024 Category: Biochemistry Authors: Lu Han Xinjie Guo Cunshuan Xu Wenlong Shen Zhihu Zhao Source Type: research

The γ-glutamyl cycle serves as an amino acids supply system in colorectal cancer organoids under chronic hypoxia
Biochem Biophys Res Commun. 2024 Apr 22;714:149977. doi: 10.1016/j.bbrc.2024.149977. Online ahead of print.ABSTRACTMalignant tumors are characterized by a hypoxic microenvironment, and metabolic reprogramming is necessary to ensure energy production and oxidative stress resistance. Although the microenvironmental properties of tumors vary under acute and chronic hypoxia, studies on chronic hypoxia-induced metabolic changes are limited. In the present study, we performed a comprehensive metabolic analysis in a chronic hypoxia model using colorectal cancer (CRC) organoids, and identified an amino acid supply system through t...
Source: Biochemical and Biophysical Research communications - April 25, 2024 Category: Biochemistry Authors: Sho Tabata Hiroko Endo Hideki Makinoshima Tomoyoshi Soga Masahiro Inoue Source Type: research

Synthesis of a tetralone derivative of ampicillin to control ampicillin-resistant Staphylococcusaureus
Biochem Biophys Res Commun. 2024 Apr 20;714:149974. doi: 10.1016/j.bbrc.2024.149974. Online ahead of print.ABSTRACTDue to the rapid emergence of antibiotic resistant new bacterial strains and new infections, there is an urgent need for novel or newly modified and efficient alternatives of treatment. However, conventional antibiotics are still used in therapeutic settings but their efficacy is uncertain due to the rapid evolution of drug resistance. In the present study, we have synthesized a new derivative of conventional antibiotic ampicillin using SN2-type substitution reaction. NMR and mass analysis of the newly synthes...
Source: Biochemical and Biophysical Research communications - April 25, 2024 Category: Biochemistry Authors: Sourav Chakraborty Piyush Baindara Suresh K Mondal Dinata Roy Santi M Mandal Source Type: research

Function analysis of transcription factor OSR1 regulating osmotic stress resistance in maize
CONCLUSIONS: OSR1 may serve as a negative regulatory factor in response to osmotic stress in maize. The present study sheds new light on the molecular mechanisms of osmotic stress in maize.PMID:38663095 | DOI:10.1016/j.bbrc.2024.149956 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 25, 2024 Category: Biochemistry Authors: Hongpeng Han Wenqiang Wu Huijiao Hou Mingli Zhang Aiyu Guo Yusen Zhou Jiong Liu Kaiwen Li Shenglong Bai Baozhu Li Zhi Li Siyi Guo Pengtao Wang Source Type: research

Investigating cryopreserved PBMC functionality in an antigen-induced model of sarcoidosis granuloma formation
This study aimed to assess the viability and functionality of cryopreserved sarcoidosis PBMCs within the granuloma model, revealing similar granulomatous responses to fresh cells and highlighting the potential of cryopreserved PBMCs as a valuable tool for studying sarcoidosis and related diseases.PMID:38663096 | DOI:10.1016/j.bbrc.2024.149993 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 25, 2024 Category: Biochemistry Authors: Sarah G Seman Sabahattin Bicer Mark W Julian Jonah R Mitchell Patrick J Kramer Elliott D Crouser Landon W Locke Source Type: research

Bqt4 affects relative movement between SPB and nucleolus in fission yeast
Biochem Biophys Res Commun. 2024 Apr 23;714:149970. doi: 10.1016/j.bbrc.2024.149970. Online ahead of print.ABSTRACTMovement dynamics in the nucleus involve various biological processes, including DNA repair, which is crucial for cancer prevention. Changes in the movement of the components of the nucleus indicate the changes in movement dynamics in the nucleus. In Schizosaccharomyces pombe, the inner nuclear membrane protein Bqt4 plays an essential role in attaching telomeres to the nuclear envelope. We observed that the deletion of bqt4+ caused a significant decrease in the mean square displacement (MSD) calculated from th...
Source: Biochemical and Biophysical Research communications - April 25, 2024 Category: Biochemistry Authors: Kaiyu Wang Hiroaki Ito Junko Kanoh Masaru Ueno Source Type: research

A mutation in the cardiac KV7.1 channel possibly disrupts interaction with Yotiao protein
Biochem Biophys Res Commun. 2024 Apr 16;714:149947. doi: 10.1016/j.bbrc.2024.149947. Online ahead of print.ABSTRACTHere, we characterized the p.Arg583His (R583H) Kv7.1 mutation, identified in two unrelated families suffered from LQT syndrome. This mutation is located in the HС-HD linker of the cytoplasmic portion of the Kv7.1 channel. This linker, together with HD helix are responsible for binding the A-kinase anchoring protein 9 (AKAP9), Yotiao. We studied the electrophysiological characteristics of the mutated channel expressed in CHO-K1 along with KCNE1 subunit and Yotiao protein, using the whole-cell patch-clamp techn...
Source: Biochemical and Biophysical Research communications - April 24, 2024 Category: Biochemistry Authors: Bowen Li Maria Karlova Han Zhang Oksana B Pustovit Lisha Mai Valery Novoseletsky Dmitry Podolyak Elena V Zaklyazminskaya Denis V Abramochkin Olga S Sokolova Source Type: research

Gestational diabetes mellitus induces congenital anomalies of the kidney and urinary tract in mice by altering RET/MAPK/ERK pathway
In this study, we established a GDM mouse model to investigate the embryological alterations and molecular mechanisms underlying the development of congenital anomalies of the kidney and urinary tract (CAKUT) affected by GDM. Our study highlights that GDM could contribute to the manifestation of CAKUT, with prevalent phenotypes characterized by isolated hydronephrosis and duplex kidney complicated with hydronephrosis in mice. Ectopic ureteric buds (UBs) and extended length of common nephric ducts (CNDs) were noted in the metanephric development stage. The expression of Ret and downstream p-ERK activity were enhanced in UBs...
Source: Biochemical and Biophysical Research communications - April 24, 2024 Category: Biochemistry Authors: Haixin Ju Minghui Yu Xuanjin Du Shanshan Xue Ningli Ye Lei Sun Xiaohui Wu Hong Xu Qian Shen Source Type: research

STING-deficiency in lung resident mesenchymal stromal cells contributes to the alleviation of LPS-induced lung injury
This study highlights that LR-MSC have a therapeutic role in acute lung injury, and we demonstrate that STING deficiency can enhance the immunomodulatory function of LR-MSC in controlling lung inflammation. Thus, STING can be used as an intervention target to enhance the therapeutic effect of MSC.PMID:38657444 | DOI:10.1016/j.bbrc.2024.149973 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 24, 2024 Category: Biochemistry Authors: Erming Zhao Jiawen Chen Dongbo Qiu Rukang Liang Di Lu Weikeng Tan Yunfei Qin Qiuli Liu Source Type: research

Binge alcohol induces NRF2-related antioxidant response in the skeletal muscle of female mice
CONCLUSION: These data suggest that acute alcohol intoxication induced markers of oxidative stress and antioxidant signaling through the NRF2 pathway and that there were effects of alcohol independent of a possible decrease in food intake caused by binge intoxication.PMID:38657445 | DOI:10.1016/j.bbrc.2024.149968 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 24, 2024 Category: Biochemistry Authors: Abigail L Tice Jennifer L Steiner Source Type: research

Crystal structures of human CD40 in complex with monoclonal antibodies dacetuzumab and bleselumab
Biochem Biophys Res Commun. 2024 Apr 18;714:149969. doi: 10.1016/j.bbrc.2024.149969. Online ahead of print.ABSTRACTCD40 is a member of the tumor necrosis factor receptor superfamily, and it is widely expressed on immune and non-immune cell types. The interaction between CD40 and the CD40 ligand (CD40L) plays an essential function in signaling, and the CD40/CD40L complex works as an immune checkpoint molecule. CD40 has become a therapeutic target, and a variety of agonistic/antagonistic anti-CD40 monoclonal antibodies (mAbs) have been developed. To better understand the mode of action of anti-CD40 mAbs, we determined the X-...
Source: Biochemical and Biophysical Research communications - April 24, 2024 Category: Biochemistry Authors: Risa Asano Makoto Nakakido Jorge Fern ández Pérez Tomoko Ise Jose M M Caaveiro Satoshi Nagata Kouhei Tsumoto Source Type: research

Transforming growth factor- β signals promote progression of squamous cell carcinoma by inducing epithelial-mesenchymal transition and angiogenesis
In this study, we examined the effects of TGF-β signaling on the murine squamous cell carcinoma, SCCVII cells in vitro and in vivo. The incubation of SCCVII cells with TGF-β induced the activation of TGF-β signals and epithelial-mesenchymal transition (EMT). Notably, the motility of SCCVII cells was increased upon the activation of the TGF-β signaling. RNA sequencing revealed upregulation of genes related to EMT and angiogenesis. Consistent with these in vitro results, the inhibition of TGF-β signals in SCCVII cell-derived primary tumors resulted in suppressed angiogenesis. Furthermore, we identified six candidate fac...
Source: Biochemical and Biophysical Research communications - April 24, 2024 Category: Biochemistry Authors: Haruka Ibi Kazuki Takahashi Hiroyuki Harada Tetsuro Watabe Katarzyna A Podyma-Inoue Source Type: research

High-resolution crystal structure of RNA kinase ArK1 from G. acetivorans
Biochem Biophys Res Commun. 2024 Apr 22;714:149966. doi: 10.1016/j.bbrc.2024.149966. Online ahead of print.ABSTRACTU47 phosphorylation (Up47) is a novel tRNA modification discovered recently; it can confer thermal stability and nuclease resistance to tRNAs. U47 phosphorylation is catalyzed by Archaeal RNA kinase (Ark1) in an ATP-dependent manner. However, the structural basis for tRNA and/or ATP binding by Ark1 is unclear. Here, we report the expression, purification, and crystallization studies of Ark1 from G. acetivorans (GaArk1). In addition to the Apo-form structure, one GaArk1-ATP complex was also determined in atomic...
Source: Biochemical and Biophysical Research communications - April 24, 2024 Category: Biochemistry Authors: Chulei Cao Weizhen Zhang Yanqing Gao Jie Yang Hehua Liu Jianhua Gan Source Type: research

Crystal structure of the 3' →5' exonuclease from Methanocaldococcus jannaschii
Biochem Biophys Res Commun. 2024 Apr 6;712-713:149893. doi: 10.1016/j.bbrc.2024.149893. Online ahead of print.ABSTRACTRecJ exonucleases are members of the DHH phosphodiesterase family ancestors of eukaryotic Cdc45, the key component of the CMG (Cdc45-MCM-GINS) complex at the replication fork. They are involved in DNA replication and repair, RNA maturation and Okazaki fragment degradation. Bacterial RecJs resect 5'-end ssDNA. Conversely, archaeal RecJs are more versatile being able to hydrolyse in both directions and acting on ssDNA as well as on RNA. In Methanocaldococcus jannaschii two RecJs were previously characterized:...
Source: Biochemical and Biophysical Research communications - April 24, 2024 Category: Biochemistry Authors: Matteo De March Source Type: research

Inhibiting Pink1/Parkin-mediated mitophagy enhances the anticancer effects of quercetin in hepatocellular carcinomaf
In this study, we aimed to explore the interplay among quercetin, mitochondrial dynamics and mitophagy and whether mitophagy-inhibition synergistically enhances the anti-tumor effects of quercetin. Huh7 and Hep3B cells were utilized for in vitro and in vivo studies. Results showed that quercetin treatment significantly increased the expression of mitochondrial fusion genes (MFN1 and MFN2) and decreased the expression of fission genes (DRP1 and FIS1) in Huh7 and Hep3B cells, leading to a more fused and elongated mitochondrial network. Quercetin upregulated the expression of key mitophagy regulators, PINK1 and PARK2, and enh...
Source: Biochemical and Biophysical Research communications - April 23, 2024 Category: Biochemistry Authors: Fang Chen Source Type: research