Knockout of Brca1-interacting factor Ola1 in female mice induces tumors with estrogen suppressible centrosome amplification
Biochim Biophys Acta Mol Basis Dis. 2024 Mar 25:167138. doi: 10.1016/j.bbadis.2024.167138. Online ahead of print.ABSTRACTObg-like ATPase 1 (OLA1) is a binding protein of Breast cancer gene 1 (BRCA1), germline pathogenic variants of which cause hereditary breast cancer. Cancer-associated variants of BRCA1 and OLA1 are deficient in the regulation of centrosome number. Although OLA1 might function as a tumor suppressor, the relevance of OLA1 deficiency to carcinogenesis is unclear. Here, we generated Ola1 knockout mice. Aged female Ola1+/- mice developed lymphoproliferative diseases, including malignant lymphoma. The lymphoma...
Source: Biochimica et Biophysica Acta - March 27, 2024 Category: Biochemistry Authors: Yuki Yoshino Honami Ogoh Yudai Iichi Tomohiro Sasaki Takahiro Yoshida Shiori Ichimura Masahiro Nakayama Wu Xi Hiroki Fujita Megumi Kikuchi Zhenzhou Fang Xingming Li Takaya Abe Mitsuru Futakuchi Yasuhiro Nakamura Toshio Watanabe Natsuko Chiba Source Type: research

CX3CR1 deficiency promotes resolution of hepatic ischemia-reperfusion injury by regulating homeostatic function of liver infiltrating macrophages
Biochim Biophys Acta Mol Basis Dis. 2024 Mar 25:167130. doi: 10.1016/j.bbadis.2024.167130. Online ahead of print.ABSTRACTHepatic ischemia-reperfusion injury(HIRI) remains to be an unsolved risk factor that contributes to organ failure after liver surgery. Our clinical retrospective study showed that lower donor liver CX3-C chemokine receptor-1(CX3CR1) mRNA expression level were correlated with upregulated pro-resolved macrophages receptor MERTK, as well as promoted restoration efficiency of allograft injury in liver transplant. To further characterize roles of CX3CR1 in regulating resolution of HIRI, we employed murine liv...
Source: Biochimica et Biophysica Acta - March 27, 2024 Category: Biochemistry Authors: Hanwen Zhang Guohua You Qing Yang Guanghui Jin Guo Lv Linda Fan Yifan Chen Huidi Li Shuhong Yi Hua Li Na Guo Wei Liu Yang Yang Source Type: research

Proteomics on human cerebral cavernous malformations reveals novel biomarkers in neurovascular dysfunction for the disease pathology
Biochim Biophys Acta Mol Basis Dis. 2024 Mar 25:167139. doi: 10.1016/j.bbadis.2024.167139. Online ahead of print.ABSTRACTBACKGROUND: Cerebral cavernous malformation (CCM) is a disease associated with elevated risk of focal neurological deficits, seizures, and hemorrhagic stroke. The disease has an inflammation profile and improved knowledge of CCM pathology mechanisms and exploration of candidate biomarkers will enable new non-invasive treatments.METHODS: We analyzed protein expression signature in human CCM tissue samples by using a highly specific and sensitive multiplexing technique, proximity extension assay.FINDINGS: ...
Source: Biochimica et Biophysica Acta - March 27, 2024 Category: Biochemistry Authors: Suvi Jauhiainen Favour C Onyeogaziri Francesca Lazzaroni Lei Liu Conze Johanna P Laakkonen Nihay Laham Karam Aki Laakso Mika Niemel ä Behnam Rezai Jahromi Peetra U Magnusson Source Type: research

The Y49H cytochrome c variant enhances megakaryocytic maturation of K-562 cells
Biochim Biophys Acta Mol Basis Dis. 2024 Mar 24:167134. doi: 10.1016/j.bbadis.2024.167134. Online ahead of print.ABSTRACTFive pathogenic variants in the gene encoding cytochrome c (CYCS) associated with mild autosomal dominant thrombocytopenia have been reported. Previous studies of peripheral blood CD34+ or CD45+ cells from subjects with the G42S CYCS variant showed an acceleration in megakaryopoiesis compared to wild-type (WT) cells. To determine whether this result reflects a common feature of the CYCS variants, the c.145T>C mutation (Y49H variant) was introduced into the endogenous CYCS locus in K-562 cells, which u...
Source: Biochimica et Biophysica Acta - March 26, 2024 Category: Biochemistry Authors: Zahra Shafaei Pishabad Elizabeth C Ledgerwood Source Type: research

Dipeptidyl-peptidase 9 regulates the dynamics of tumorigenesis and metastasis in breast cancer
Biochim Biophys Acta Mol Basis Dis. 2024 Mar 24:167133. doi: 10.1016/j.bbadis.2024.167133. Online ahead of print.ABSTRACTThe cytosolic dipeptidyl-aminopeptidase 9 (DPP9) cleaves protein N-termini post-proline or -alanine. Our analysis of DPP9 mRNA expression from the TCGA 'breast cancer' data set revealed that low/intermediate DPP9 levels are associated with poor overall survival of breast cancer patients. To unravel the impact of DPP9 on breast cancer development and progression, the transgenic MMTV-PyMT mouse model of metastasizing breast cancer was used. In addition, tissue- and time-controlled genetic deletion of DPP9 ...
Source: Biochimica et Biophysica Acta - March 26, 2024 Category: Biochemistry Authors: Lisa He ß Kazeera Aliar Barbara T Gr ünwald Ricarda Griffin Alina Lozan Mariel Kn öller Rama Khokha Tilman Brummer Thomas Reinheckel Source Type: research

FXR/Menin-mediated epigenetic regulation of E2F3 expression controls β-cell proliferation and is increased in islets from diabetic GK rats after RYGB
Biochim Biophys Acta Mol Basis Dis. 2024 Mar 24:167136. doi: 10.1016/j.bbadis.2024.167136. Online ahead of print.ABSTRACTFarnesoid X receptor (FXR) improves the function of islets, especially in the setting of Roux-en-Y gastric bypass (RYGB). Here we investigated how FXR activation regulates β-cell proliferation and explored the potential link between FXR signaling and the menin pathway in controlling E2F3 expression, a key transcription factor for controlling adult β-cell proliferation. Stimulation with the FXR agonist GW4064 or chenodeoxycholic acid (CDCA) increased E2F3 expression and β-cell proliferation. Consistent...
Source: Biochimica et Biophysica Acta - March 26, 2024 Category: Biochemistry Authors: Xiangchen Kong Chenxi Yang Bingfeng Li Dan Yan Yanhui Yang Cuihua Cao Bowen Xing Xiaosong Ma Source Type: research

The Y49H cytochrome c variant enhances megakaryocytic maturation of K-562 cells
Biochim Biophys Acta Mol Basis Dis. 2024 Mar 24:167134. doi: 10.1016/j.bbadis.2024.167134. Online ahead of print.ABSTRACTFive pathogenic variants in the gene encoding cytochrome c (CYCS) associated with mild autosomal dominant thrombocytopenia have been reported. Previous studies of peripheral blood CD34+ or CD45+ cells from subjects with the G42S CYCS variant showed an acceleration in megakaryopoiesis compared to wild-type (WT) cells. To determine whether this result reflects a common feature of the CYCS variants, the c.145T>C mutation (Y49H variant) was introduced into the endogenous CYCS locus in K-562 cells, which u...
Source: Biochimica et Biophysica Acta - March 26, 2024 Category: Biochemistry Authors: Zahra Shafaei Pishabad Elizabeth C Ledgerwood Source Type: research

Dipeptidyl-peptidase 9 regulates the dynamics of tumorigenesis and metastasis in breast cancer
Biochim Biophys Acta Mol Basis Dis. 2024 Mar 24:167133. doi: 10.1016/j.bbadis.2024.167133. Online ahead of print.ABSTRACTThe cytosolic dipeptidyl-aminopeptidase 9 (DPP9) cleaves protein N-termini post-proline or -alanine. Our analysis of DPP9 mRNA expression from the TCGA 'breast cancer' data set revealed that low/intermediate DPP9 levels are associated with poor overall survival of breast cancer patients. To unravel the impact of DPP9 on breast cancer development and progression, the transgenic MMTV-PyMT mouse model of metastasizing breast cancer was used. In addition, tissue- and time-controlled genetic deletion of DPP9 ...
Source: Biochimica et Biophysica Acta - March 26, 2024 Category: Biochemistry Authors: Lisa He ß Kazeera Aliar Barbara T Gr ünwald Ricarda Griffin Alina Lozan Mariel Kn öller Rama Khokha Tilman Brummer Thomas Reinheckel Source Type: research

FXR/Menin-mediated epigenetic regulation of E2F3 expression controls β-cell proliferation and is increased in islets from diabetic GK rats after RYGB
Biochim Biophys Acta Mol Basis Dis. 2024 Mar 24:167136. doi: 10.1016/j.bbadis.2024.167136. Online ahead of print.ABSTRACTFarnesoid X receptor (FXR) improves the function of islets, especially in the setting of Roux-en-Y gastric bypass (RYGB). Here we investigated how FXR activation regulates β-cell proliferation and explored the potential link between FXR signaling and the menin pathway in controlling E2F3 expression, a key transcription factor for controlling adult β-cell proliferation. Stimulation with the FXR agonist GW4064 or chenodeoxycholic acid (CDCA) increased E2F3 expression and β-cell proliferation. Consistent...
Source: Biochimica et Biophysica Acta - March 26, 2024 Category: Biochemistry Authors: Xiangchen Kong Chenxi Yang Bingfeng Li Dan Yan Yanhui Yang Cuihua Cao Bowen Xing Xiaosong Ma Source Type: research

Polyamine release and vesicular polyamine transporter expression in megakaryoblastic cells and platelets
In this study, we investigated the possible involvement of VPAT in vesicular release of polyamines in MEG-01 clonal megakaryoblastic cells and platelets. RT-PCR, western blotting, and immunohistochemistry revealed VPAT expression in MEG-01 cells. MEG-01 cells secreted polyamines upon A23187 stimulation in the presence of Ca2+, which is temperature-dependent and sensitive to bafilomycin A1. A23187-induced polyamine secretion from MEG-01 cells was reduced by treatment with reserpine, VPAT inhibitors, or VPAT RNA interference. Platelets also expressed VPAT, displaying a punctate distribution, and released spermidine upon A231...
Source: Biochimica et Biophysica Acta - March 25, 2024 Category: Biochemistry Authors: Mizuki Uehara Ayaka Fukumoto Hiroshi Omote Miki Hiasa Source Type: research

Hippocampal HDAC6 promotes POCD by regulating NLRP3-induced microglia pyroptosis via HSP90/HSP70 in aged mice
CONCLUSIONS: Our findings suggest that hippocampal HDAC6 promotes POCD by regulating NLRP3-induced microglia pyroptosis via HSP90/HSP70 in aged mice.PMID:38527593 | DOI:10.1016/j.bbadis.2024.167137 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - March 25, 2024 Category: Biochemistry Authors: Qi-Cheng Lin Jiao Wang Xin-Lin Wang Chi Pan Shao-Wu Jin Steven Char Yuan-Xiang Tao Hong Cao Jun Li Source Type: research

The unusual substrate specificity of Escherichia coli cardiolipin synthase C does not require the product of the transcriptionally engaged ymdB gene
Biochim Biophys Acta Mol Cell Biol Lipids. 2024 Mar 23:159483. doi: 10.1016/j.bbalip.2024.159483. Online ahead of print.ABSTRACT- ClsC drives cardiolipin (CL) synthesis even when YmdB is not transcriptionally paired. Polycistronic transcription and translation of ymdB-clsC which has been thought to be required for full activity of ClsC is not necessary for CL synthesis or the substrate specificity of ClsC, which condenses phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The authentic initiation codon of the clsC gene is present within the open reading frame of the upstream located ymdB gene. - Initiation of tra...
Source: Biochimica et Biophysica Acta - March 25, 2024 Category: Biochemistry Authors: Katsuhiro Sawasato Mikhail Bogdanov Source Type: research

Elements of the C-terminal tail of a C-terminal domain homolog of the Orange Carotenoid Protein determining xanthophyll uptake from liposomes
Biochim Biophys Acta Bioenerg. 2024 Mar 22:149043. doi: 10.1016/j.bbabio.2024.149043. Online ahead of print.ABSTRACTCarotenoids perform multifaceted roles in life ranging from coloration over light harvesting to photoprotection. The Orange Carotenoid Protein (OCP), a light-driven photoswitch involved in cyanobacterial photoprotection, accommodates a ketocarotenoid vital for its function. OCP extracts its ketocarotenoid directly from membranes, or accepts it from homologs of its C-terminal domain (CTDH). The CTDH from Anabaena (AnaCTDH) was shown to be important for carotenoid transfer and delivery from/to membranes. The C-...
Source: Biochimica et Biophysica Acta - March 24, 2024 Category: Biochemistry Authors: Kristina Likkei Marcus Moldenhauer Neslihan N Tavraz Nikita A Egorkin Yury B Slonimskiy Eugene G Maksimov Nikolai N Sluchanko Thomas Friedrich Source Type: research

Enhancing subcellular protein localization mapping analysis using Sc2promap utilizing attention mechanisms
CONCLUSIONS: The amalgamation of the attention mechanism and prediction head components has led the model to excel in protein localization clustering and analysis tasks.GENERAL SIGNIFICANCE: The model effectively enhances the capability to extract features and knowledge from protein fluorescence images.PMID:38522679 | DOI:10.1016/j.bbagen.2024.130601 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - March 24, 2024 Category: Biochemistry Authors: Kaitai Han Xi Liu Guocheng Sun Zijun Wang Chaojing Shi Wu Liu Mengyuan Huang Shitou Liu Qianjin Guo Source Type: research

The importance of Ca < sup > 2+ < /sup > microdomains for the adaptive immune response
Biochim Biophys Acta Mol Cell Res. 2024 Mar 22:119710. doi: 10.1016/j.bbamcr.2024.119710. Online ahead of print.ABSTRACTCalcium signaling stands out as the most widespread and universally used signaling system and is of utmost importance for immunity. Controlled elevations in cytosolic and organellar Ca2+ concentrations in T cells control complex and essential effector functions including proliferation, differentiation, cytokine secretion, and cytotoxicity, among others. Additionally, disruptions in Ca2+ regulation in T cells contribute to diverse autoimmune, inflammatory, and immunodeficiency conditions. Among the initial...
Source: Biochimica et Biophysica Acta - March 24, 2024 Category: Biochemistry Authors: Bj örn-Philipp Diercks Source Type: research