The impact of chemotherapy on adipose tissue remodeling: The molecular players involved in this tissue wasting
Biochimie. 2024 Mar 25:S0300-9084(24)00074-9. doi: 10.1016/j.biochi.2024.03.016. Online ahead of print.ABSTRACTThe depletion of visceral and subcutaneous adipose tissue (AT) during chemotherapy significantly correlates with diminished overall survival and progression-free survival. Despite its clinical significance, the intricate molecular mechanisms governing this AT loss and its chemotherapy-triggered initiation remain poorly understood. Notably, the evaluation of AT remodeling in most clinical trials has predominantly relied on computerized tomography scans or bioimpedance, with molecular studies often conducted using a...
Source: Biochimie - March 27, 2024 Category: Biochemistry Authors: Samuel Barbosa Mafalda Barbosa Pedrosa Rita Ferreira Daniel Moreira-Gon çalves L úcio Lara Santos Source Type: research

Inhibition of soluble epoxide hydrolase as a therapeutic approach for blood-brain barrier dysfunction
Biochimie. 2024 Mar 24:S0300-9084(24)00073-7. doi: 10.1016/j.biochi.2024.03.015. Online ahead of print.ABSTRACTThe blood-brain barrier (BBB) is a protective semi-permeable structure that regulates the exchange of biomolecules between the peripheral blood and the central nervous system (CNS). Due to its specialized tight junctions and low vesicle trafficking, the BBB strictly limits the paracellular passage and transcellular transport of molecules to maintain the physiological condition of brain tissues. BBB breakdown is associated with many CNS disorders. Soluble epoxide hydrolase (sEH) is a hydrolase enzyme that converts ...
Source: Biochimie - March 26, 2024 Category: Biochemistry Authors: Shuo Li Huijia Song Yanping Sun Yongjun Sun Huimin Zhang Zibin Gao Source Type: research

Inhibition of soluble epoxide hydrolase as a therapeutic approach for blood-brain barrier dysfunction
Biochimie. 2024 Mar 24:S0300-9084(24)00073-7. doi: 10.1016/j.biochi.2024.03.015. Online ahead of print.ABSTRACTThe blood-brain barrier (BBB) is a protective semi-permeable structure that regulates the exchange of biomolecules between the peripheral blood and the central nervous system (CNS). Due to its specialized tight junctions and low vesicle trafficking, the BBB strictly limits the paracellular passage and transcellular transport of molecules to maintain the physiological condition of brain tissues. BBB breakdown is associated with many CNS disorders. Soluble epoxide hydrolase (sEH) is a hydrolase enzyme that converts ...
Source: Biochimie - March 26, 2024 Category: Biochemistry Authors: Shuo Li Huijia Song Yanping Sun Yongjun Sun Huimin Zhang Zibin Gao Source Type: research

Thrombin stories in the gut
Biochimie. 2024 Mar 21:S0300-9084(24)00065-8. doi: 10.1016/j.biochi.2024.03.007. Online ahead of print.ABSTRACTMany studies have demonstrated the involvement of proteases in gut physiology and pathophysiology over the recent years. Among them, thrombin has appeared for a long time as an old player only involved in blood clotting upon tissue injury. The fact that thrombin receptors (Protease-Activated Receptors-1 and -4) are expressed and functional in almost all cell types of the gut, contributing to barrier, immune or motility functions, suggested that thrombin could actually be at the crossroad of intestinal physiology. ...
Source: Biochimie - March 23, 2024 Category: Biochemistry Authors: Nathalie Vergnolle Source Type: research

Thrombin stories in the gut
Biochimie. 2024 Mar 21:S0300-9084(24)00065-8. doi: 10.1016/j.biochi.2024.03.007. Online ahead of print.ABSTRACTMany studies have demonstrated the involvement of proteases in gut physiology and pathophysiology over the recent years. Among them, thrombin has appeared for a long time as an old player only involved in blood clotting upon tissue injury. The fact that thrombin receptors (Protease-Activated Receptors-1 and -4) are expressed and functional in almost all cell types of the gut, contributing to barrier, immune or motility functions, suggested that thrombin could actually be at the crossroad of intestinal physiology. ...
Source: Biochimie - March 23, 2024 Category: Biochemistry Authors: Nathalie Vergnolle Source Type: research

Thrombin stories in the gut
Biochimie. 2024 Mar 21:S0300-9084(24)00065-8. doi: 10.1016/j.biochi.2024.03.007. Online ahead of print.ABSTRACTMany studies have demonstrated the involvement of proteases in gut physiology and pathophysiology over the recent years. Among them, thrombin has appeared for a long time as an old player only involved in blood clotting upon tissue injury. The fact that thrombin receptors (Protease-Activated Receptors-1 and -4) are expressed and functional in almost all cell types of the gut, contributing to barrier, immune or motility functions, suggested that thrombin could actually be at the crossroad of intestinal physiology. ...
Source: Biochimie - March 23, 2024 Category: Biochemistry Authors: Nathalie Vergnolle Source Type: research

Matrix metalloproteinase landscape in the imiquimod-induced skin inflammation mouse model
In this study, we investigated the abundance of a subset of matrix metalloproteinases (MMPs) in skin from mice with IMQ-induced skin inflammation and skin from naïve mice using targeted proteomics. Our findings reveal a significant increase in the abundance of MMP-2, MMP-7, MMP-8, and MMP-13 after treatment with IMQ compared to the control skin, while MMP-3, MMP-9, and MMP-10 were exclusively detected in the IMQ-treated skin. The increased abundance and broader representation of MMPs in the IMQ-treated skin provide valuable insight into the pathophysiology of skin inflammation in the IMQ model, adding to previous studies ...
Source: Biochimie - March 21, 2024 Category: Biochemistry Authors: Heidi Kyung Noddeland Vahap Canbay Marianne Lind Simonas Savickas Louise Bastholm Jensen Karsten Petersson Martin Malmsten Janne Koch Ulrich Auf dem Keller Andrea Heinz Source Type: research

Matrix metalloproteinase landscape in the imiquimod-induced skin inflammation mouse model
In this study, we investigated the abundance of a subset of matrix metalloproteinases (MMPs) in skin from mice with IMQ-induced skin inflammation and skin from naïve mice using targeted proteomics. Our findings reveal a significant increase in the abundance of MMP-2, MMP-7, MMP-8, and MMP-13 after treatment with IMQ compared to the control skin, while MMP-3, MMP-9, and MMP-10 were exclusively detected in the IMQ-treated skin. The increased abundance and broader representation of MMPs in the IMQ-treated skin provide valuable insight into the pathophysiology of skin inflammation in the IMQ model, adding to previous studies ...
Source: Biochimie - March 21, 2024 Category: Biochemistry Authors: Heidi Kyung Noddeland Vahap Canbay Marianne Lind Simonas Savickas Louise Bastholm Jensen Karsten Petersson Martin Malmsten Janne Koch Ulrich Auf dem Keller Andrea Heinz Source Type: research

Melatonin facts: Melatonin lacks immuno-inflammation boosting capacities at the molecular and cellular levels
Biochimie. 2024 Mar 18:S0300-9084(24)00068-3. doi: 10.1016/j.biochi.2024.03.010. Online ahead of print.ABSTRACTAmong the properties melatonin is claimed to possess, are the immuno-inflammation inductive capacities that would be responsible of some of the paramount of activities melatonin is reported to have in most of the human pathological conditions. In the present paper, we measured the effect of melatonin on established cellular models of immuno-inflammation, and found none. The discrepancies are discussed, especially because those properties are reported at pharmacological concentration (1 μM and beyond) at which the...
Source: Biochimie - March 20, 2024 Category: Biochemistry Authors: Jean A Boutin Val érie Hamon de Almeida Nathalie Coussay C éline Legros Gilles Ferry Karine Reybier Source Type: research

Melatonin facts: Melatonin lacks immuno-inflammation boosting capacities at the molecular and cellular levels
Biochimie. 2024 Mar 18:S0300-9084(24)00068-3. doi: 10.1016/j.biochi.2024.03.010. Online ahead of print.ABSTRACTAmong the properties melatonin is claimed to possess, are the immuno-inflammation inductive capacities that would be responsible of some of the paramount of activities melatonin is reported to have in most of the human pathological conditions. In the present paper, we measured the effect of melatonin on established cellular models of immuno-inflammation, and found none. The discrepancies are discussed, especially because those properties are reported at pharmacological concentration (1 μM and beyond) at which the...
Source: Biochimie - March 20, 2024 Category: Biochemistry Authors: Jean A Boutin Val érie Hamon de Almeida Nathalie Coussay C éline Legros Gilles Ferry Karine Reybier Source Type: research

The multifaceted role of proteases and modern analytical methods for investigation of their catalytic activity
Biochimie. 2024 Mar 15:S0300-9084(24)00064-6. doi: 10.1016/j.biochi.2024.03.006. Online ahead of print.ABSTRACTWe discuss the diverse functions of proteases in the context of their biotechnological and medical significance, as well as analytical approaches used to determine the functional activity of these enzymes. An insight into modern approaches to studying the kinetics and specificity of proteases, based on spectral (absorption, fluorescence), mass spectrometric, immunological, calorimetric, and electrochemical methods of analysis is given. We also examine in detail electrochemical systems for determining the activity ...
Source: Biochimie - March 17, 2024 Category: Biochemistry Authors: Tatiana A Filippova Rami A Masamrekh Yulia Yu Khudoklinova Victoria V Shumyantseva Alexey V Kuzikov Source Type: research

Short-term exposure to cigarette smoke upregulates cathepsin S and alters expression of tight junction ZO-1
Biochimie. 2024 Mar 15:S0300-9084(24)00063-4. doi: 10.1016/j.biochi.2024.03.005. Online ahead of print.ABSTRACTA long-term exposure to cigarette smoke (CS) alters the integrity of airway epithelial barrier, contributes to lung dysfunction, and elicits the expression and activity of lung cathepsin S (CatS), a cysteine protease that participates in the remodeling of connective tissue and cell junctions. Here, we observed that a short-term (4 days) exposure of mice to CS increased the expression and activity of CatS, while the expression level of zonula occludens 1 (ZO-1), an epithelial tight junction protein that stabilizes ...
Source: Biochimie - March 17, 2024 Category: Biochemistry Authors: Florent Estur Emilie Murigneux Alexis David M élia Magnen Ahlame Saidi Gilles Lalmanach Fabien Lecaille Source Type: research

The mutual and dynamic role of TSPO and ligands in their binding process: An example with PK-11195
Biochimie. 2024 Mar 15:S0300-9084(24)00067-1. doi: 10.1016/j.biochi.2024.03.009. Online ahead of print.ABSTRACTTranslocator protein (TSPO) is an 18 kDa transmembrane protein, localized primarily on the outer mitochondrial membrane. It has been found to be involved in various physiological processes and pathophysiological conditions. Though studies on its structure have been performed only recently, there is little information on the nature of dynamics and doubts about some structures referenced in the literature, especially the NMR structure of mouse TSPO. In the present work, we thoroughly study the dynamics of mouse TSPO...
Source: Biochimie - March 17, 2024 Category: Biochemistry Authors: Rajas M Rao Ibaa El Dhaybi Fr édéric Cadet Catherine Etchebest Julien Diharce Source Type: research

The molecular crosstalk of the hippo cascade in breast cancer: A potential central susceptibility
Biochimie. 2024 Mar 15:S0300-9084(24)00066-X. doi: 10.1016/j.biochi.2024.03.008. Online ahead of print.ABSTRACTThe incidence of breast cancer is perpetually growing globally, and it remains a major public health problem and the leading cause of mortality in women. Though the aberrant activities of the Hippo pathway have been reported to be associated with cancer, constructive knowledge of the pathway connecting the various elements of breast cancer remains to be elucidated. The Hippo transducers, yes-associated protein (YAP) and transcriptional co-activator with PDZ binding motif (TAZ), are reported to be either tumor supp...
Source: Biochimie - March 17, 2024 Category: Biochemistry Authors: Sulfath Thottungal Parambil Gisha Rose Antony Ajeesh Babu Littleflower Lakshmi Subhadradevi Source Type: research

Unraveling the impact of the p.R107L mutation on the structure and function of human αB-Crystallin: Implications for cataract formation
In this study, we investigated the structure, chaperone function, stability, oligomerization, and amyloidogenic properties of the p.R107L human αB-Crystallin using a number of different techniques. Our results suggest that the p.R107L mutation can cause significant changes in the secondary, tertiary, and quaternary structures of αB-Crystallin. This cataractogenic mutation led to the formation of protein oligomers with larger sizes than the wild-type protein and reduced the chemical and thermal stability of the mutant chaperone. Both fluorescence and microscopic assessments indicated that this mutation significantly alter...
Source: Biochimie - March 17, 2024 Category: Biochemistry Authors: Farid Nasiri Parisa Ebrahimi Mohammad Bagher Shahsavani Anis Barati Issa Zarei Jun Hong Masaru Hoshino Ali Akbar Moosavi-Movahedi Reza Yousefi Source Type: research