Enhanced recognition of G-quadruplex DNA oxidative damage based on DNA-mediated charge transfer
Bioelectrochemistry. 2024 Apr 21;158:108714. doi: 10.1016/j.bioelechem.2024.108714. Online ahead of print.ABSTRACTG-quadruplex (G4) DNA is present in human telomere oligonucleotide sequences. Oxidative damage to telomeric DNA accelerates telomere shortening, which is strongly associated with aging and cancer. Most of the current analyses on oxidative DNA damage are based on ds-DNA. Here, we developed a electrochemiluminescence (ECL) probe for enhanced recognition of oxidative damage in G4-DNA based on DNA-mediated charge transfer (CT), which could specifically recognize damaged sites depending on the position of 8-oxoguani...
Source: Bioelectrochemistry - April 23, 2024 Category: Biochemistry Authors: Zhangjin He Ziqi Wang Liping Lu Xiayan Wang Guangsheng Guo Source Type: research
Continuous high-purity bioelectrochemical nitrogen recovery from high N-loaded wastewaters
Bioelectrochemistry. 2024 Apr 16;158:108707. doi: 10.1016/j.bioelechem.2024.108707. Online ahead of print.ABSTRACTMicrobial electrolysis cells (MEC) have been identified as an energy efficient system for ammonium recovery from wastewater. However, high ammonium concentrations at the anode can have inhibitory effects. This work aims to determine the effects on current generation performance and active ammonia nitrogen recovery in wastewater containing 0.5 to 2.5 g N-NH4+/L. The study also evaluates the effect of two cathode materials, stainless steel (SS-MEC) and nickel foam (NF-MEC). When the concentration of ammonium in t...
Source: Bioelectrochemistry - April 23, 2024 Category: Biochemistry Authors: Zainab Ul Mira Sulonen Juan Antonio Baeza Albert Guisasola Source Type: research
Singlet oxygen-based photoelectrochemical detection of miRNAs in prostate cancer patients' plasma: A novel diagnostic tool for liquid biopsy
Bioelectrochemistry. 2024 Apr 4;158:108698. doi: 10.1016/j.bioelechem.2024.108698. Online ahead of print.ABSTRACTDysregulation of miRNA expression occurs in many cancers, making miRNAs useful in cancer diagnosis and therapeutic guidance. In a clinical context using methods such as polymerase chain reaction (PCR), the limited amount of miRNAs in circulation often limits their quantification. Here, we present a PCR-free and sensitive singlet oxygen (1O2)-based strategy for the detection and quantification of miRNAs in untreated human plasma from patients diagnosed with prostate cancer. A target miRNA is specifically captured...
Source: Bioelectrochemistry - April 19, 2024 Category: Biochemistry Authors: Saranya Thiruvottriyur Shanmugam Rui Campos Stanislav Trashin Elise Daems Diogo Carneiro Avelino Fraga Ricardo Ribeiro Karolien De Wael Source Type: research
Revealing cellular (poly)sulphide storage in electrochemically active sulphide oxidising bacteria using rotating disc electrodes
Bioelectrochemistry. 2024 Apr 14;158:108710. doi: 10.1016/j.bioelechem.2024.108710. Online ahead of print.ABSTRACTSulphide oxidising bacteria (SOB) have the potential to be used for bioelectrochemical removal, i.e. oxidation, of sulphide from waste streams. In anaerobic conditions, SOB are able to spatially separate sulphide removal and terminal electron transfer to an electrode and act as a sulphide shuttle. However, it is not fully understood how SOB anaerobically remove sulphide and store charge equivalents, and where in this process sulphur is formed. Therefore, the redox behaviour of sulphide shuttling SOB was investi...
Source: Bioelectrochemistry - April 18, 2024 Category: Biochemistry Authors: Rikke Linssen Sanne de Smit Katharina R öhring Neé Neubert Falk Harnisch Annemiek Ter Heijne Source Type: research
Implementation of π-π interaction in AuNPs@GDY to boost the bioelectrocatalysis in enzymatic biofuel cells
Bioelectrochemistry. 2024 Apr 15;158:108712. doi: 10.1016/j.bioelechem.2024.108712. Online ahead of print.ABSTRACTThe main challenges (sluggish electron transfer, low energy density) hinder the future application of enzymatic biofuel cells (EBFCs), which urgent to take effective measures to solve these issues. In this work, a composite of Au nanoparticles decorated graphdiyne (AuNPs@GDY) is fabricated and employed as the carrier of enzyme (G6PDH), and a mechanism based on π-π interaction of electron transfer is proposed to understand bioelectrocatalysis processes. The results show that the AuNPs@GDY composite exhibits th...
Source: Bioelectrochemistry - April 18, 2024 Category: Biochemistry Authors: Zhikai Wang Yunpeng Li Zhuanzhuan Shi Xiaohai Wang Chunxian Guo Chang Ming Li Xiaoshuai Wu Source Type: research
The comparison of the dynamics of Ca < sup > 2+ < /sup > and bleomycin intracellular delivery after cell sonoporation and electroporation in vitro
Bioelectrochemistry. 2024 Apr 14;158:108708. doi: 10.1016/j.bioelechem.2024.108708. Online ahead of print.ABSTRACTCa2+, in combination with SP or EP, induces cell cytotoxicity much faster compared to BLM. The application of BLM in combination with, SP or EP, reaches the level of cell death, induced by similar combination with Ca2+, only after 72 h. The methods of SP and EP were calibrated according to the level of differential cytotoxicity, determined after 6 days (using cell clonogenic assay). The combination of Ca2+ SP induces cell death faster than Ca2+ EP - after Ca2+ SP it increases to a maximum level after 15 min and...
Source: Bioelectrochemistry - April 18, 2024 Category: Biochemistry Authors: Martynas Maciulevi čius R ūta Palepšienė Salvijus Vykertas Renaldas Rai šutis Aras Rafanavi čius Tomas Krilavi čius Saulius Šatkauskas Source Type: research
Anaerobic treatment of groundwater co-contaminated by toluene and copper in a single chamber bioelectrochemical system
Bioelectrochemistry. 2024 Apr 13;158:108711. doi: 10.1016/j.bioelechem.2024.108711. Online ahead of print.ABSTRACTAddressing the simultaneous removal of multiple coexisting groundwater contaminants poses a significant challenge, primarily because of their different physicochemical properties. Indeed, different chemical compounds may necessitate establishing distinct, and sometimes conflicting, (bio)degradation and/or removal pathways. In this work, we investigated the concomitant anaerobic treatment of toluene and copper in a single-chamber bioelectrochemical cell with a potential difference of 1 V applied between the anod...
Source: Bioelectrochemistry - April 16, 2024 Category: Biochemistry Authors: Marco Resitano Matteo Tucci Alessio Mezzi Saulius Kaciulis Bruna Matturro Emilio D'Ugo Lucia Bertuccini Stefano Fazi Simona Rossetti Federico Aulenta Carolina Cruz Viggi Source Type: research
Anaerobic treatment of groundwater co-contaminated by toluene and copper in a single chamber bioelectrochemical system
Bioelectrochemistry. 2024 Apr 13;158:108711. doi: 10.1016/j.bioelechem.2024.108711. Online ahead of print.ABSTRACTAddressing the simultaneous removal of multiple coexisting groundwater contaminants poses a significant challenge, primarily because of their different physicochemical properties. Indeed, different chemical compounds may necessitate establishing distinct, and sometimes conflicting, (bio)degradation and/or removal pathways. In this work, we investigated the concomitant anaerobic treatment of toluene and copper in a single-chamber bioelectrochemical cell with a potential difference of 1 V applied between the anod...
Source: Bioelectrochemistry - April 16, 2024 Category: Biochemistry Authors: Marco Resitano Matteo Tucci Alessio Mezzi Saulius Kaciulis Bruna Matturro Emilio D'Ugo Lucia Bertuccini Stefano Fazi Simona Rossetti Federico Aulenta Carolina Cruz Viggi Source Type: research
Anaerobic treatment of groundwater co-contaminated by toluene and copper in a single chamber bioelectrochemical system
Bioelectrochemistry. 2024 Apr 13;158:108711. doi: 10.1016/j.bioelechem.2024.108711. Online ahead of print.ABSTRACTAddressing the simultaneous removal of multiple coexisting groundwater contaminants poses a significant challenge, primarily because of their different physicochemical properties. Indeed, different chemical compounds may necessitate establishing distinct, and sometimes conflicting, (bio)degradation and/or removal pathways. In this work, we investigated the concomitant anaerobic treatment of toluene and copper in a single-chamber bioelectrochemical cell with a potential difference of 1 V applied between the anod...
Source: Bioelectrochemistry - April 16, 2024 Category: Biochemistry Authors: Marco Resitano Matteo Tucci Alessio Mezzi Saulius Kaciulis Bruna Matturro Emilio D'Ugo Lucia Bertuccini Stefano Fazi Simona Rossetti Federico Aulenta Carolina Cruz Viggi Source Type: research
A dual-signal output electrochemical aptasensor for glypican-3 ultrasensitive detection based on reduced graphene oxide-cuprous oxide nanozyme catalytic amplification strategy
Bioelectrochemistry. 2024 Apr 13;158:108709. doi: 10.1016/j.bioelechem.2024.108709. Online ahead of print.ABSTRACTGlypican-3 (GPC3) is an essential reference target for hepatocellular carcinoma detection, follow-up and prediction. Herein, a dual-signal electrochemical aptasensor based on reduced graphene oxide-cuprous oxide (RGO-Cu2O) nanozyme was developed for GPC3 detection. The RGO-Cu2O nanoenzyme displayed excellent electron transport effect, large specific surface area and outstanding peroxidase-like ability. The differential pulse voltammetry (DPV) signal of Cu2O oxidation fraction and the chronoamperometry (i-t) sig...
Source: Bioelectrochemistry - April 15, 2024 Category: Biochemistry Authors: Guiyin Li Huafu Feng Xinhao Li Shengnan Li Jintao Liang Zhide Zhou Source Type: research
A dual-signal output electrochemical aptasensor for glypican-3 ultrasensitive detection based on reduced graphene oxide-cuprous oxide nanozyme catalytic amplification strategy
Bioelectrochemistry. 2024 Apr 13;158:108709. doi: 10.1016/j.bioelechem.2024.108709. Online ahead of print.ABSTRACTGlypican-3 (GPC3) is an essential reference target for hepatocellular carcinoma detection, follow-up and prediction. Herein, a dual-signal electrochemical aptasensor based on reduced graphene oxide-cuprous oxide (RGO-Cu2O) nanozyme was developed for GPC3 detection. The RGO-Cu2O nanoenzyme displayed excellent electron transport effect, large specific surface area and outstanding peroxidase-like ability. The differential pulse voltammetry (DPV) signal of Cu2O oxidation fraction and the chronoamperometry (i-t) sig...
Source: Bioelectrochemistry - April 15, 2024 Category: Biochemistry Authors: Guiyin Li Huafu Feng Xinhao Li Shengnan Li Jintao Liang Zhide Zhou Source Type: research
Optimised start-up strategy for bioelectrochemical systems operating on hydrolysed human urine
This study investigated a new strategy for bioanode inoculation by mixing real hydrolysed urine with thickened waste activated sludge (TWAS) from a municipal wastewater treatment plant at different volumetric ratios. The addition of TWAS diluted the high TAN concentration of hydrolysed urine (5.2 ± 0.3 g L-1) to 2.6-5.1 g L-1, while the pH of the inoculation mixtures remained > 9 and soluble chemical oxygen demand (sCOD) at 5.6-6.7 g L-1. Despite the high pH, current generation started within 24 h for all reactors, and robust bioanodes tolerant of continuous feeding with undiluted hydrolysed urine were enriched within ...
Source: Bioelectrochemistry - April 12, 2024 Category: Biochemistry Authors: Veera Koskue Stefano Freguia Source Type: research
Optimised start-up strategy for bioelectrochemical systems operating on hydrolysed human urine
This study investigated a new strategy for bioanode inoculation by mixing real hydrolysed urine with thickened waste activated sludge (TWAS) from a municipal wastewater treatment plant at different volumetric ratios. The addition of TWAS diluted the high TAN concentration of hydrolysed urine (5.2 ± 0.3 g L-1) to 2.6-5.1 g L-1, while the pH of the inoculation mixtures remained > 9 and soluble chemical oxygen demand (sCOD) at 5.6-6.7 g L-1. Despite the high pH, current generation started within 24 h for all reactors, and robust bioanodes tolerant of continuous feeding with undiluted hydrolysed urine were enriched within ...
Source: Bioelectrochemistry - April 12, 2024 Category: Biochemistry Authors: Veera Koskue Stefano Freguia Source Type: research
Effect of Halomonas titanicae on fluctuating water-line corrosion of EH40 steel
Bioelectrochemistry. 2024 Apr 4;158:108703. doi: 10.1016/j.bioelechem.2024.108703. Online ahead of print.ABSTRACTThe fluctuating water-line corrosion of EH40 steel in sterile and biotic media was investigated with a wire beam electrode. When the coupons were partially immersed in the sterile medium, the position of the low water-line acted as the cathodic zone and the area below the low water-line constantly served as the main anodic zone. The thin electrolyte layers with uneven thickness promoted the galvanic current of the region below the low water-line. Different from the sterile environment, the metabolism of Halomona...
Source: Bioelectrochemistry - April 10, 2024 Category: Biochemistry Authors: Ce Li Jiajia Wu Peng Wang Dun Zhang Liyang Zhu Yaohua Gao Wenkai Wang Hanzhe Sun Source Type: research
Effect of Halomonas titanicae on fluctuating water-line corrosion of EH40 steel
Bioelectrochemistry. 2024 Apr 4;158:108703. doi: 10.1016/j.bioelechem.2024.108703. Online ahead of print.ABSTRACTThe fluctuating water-line corrosion of EH40 steel in sterile and biotic media was investigated with a wire beam electrode. When the coupons were partially immersed in the sterile medium, the position of the low water-line acted as the cathodic zone and the area below the low water-line constantly served as the main anodic zone. The thin electrolyte layers with uneven thickness promoted the galvanic current of the region below the low water-line. Different from the sterile environment, the metabolism of Halomona...
Source: Bioelectrochemistry - April 10, 2024 Category: Biochemistry Authors: Ce Li Jiajia Wu Peng Wang Dun Zhang Liyang Zhu Yaohua Gao Wenkai Wang Hanzhe Sun Source Type: research
