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Localisation of translation initiation factors to talin/{beta}3-integrin-enriched adhesion complexes in spreading and migrating mammalian cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions Taken together, these findings demonstrate that repression, complex post-transcriptional regulation and modulation of mRNA stability could potentially be taking place along the distal edge of migrating lamellipodia. (Source: Biology of the Cell)
Source: Biology of the Cell - November 20, 2009 Category: Cytology Authors: M Willett, H J Pollard, M Vlasak, S J Morley Source Type: journals

Polarized traffic towards the cell surface: how to find the route.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Polarity is the structural and functional hallmark of epithelia. The apical plasma membrane, facing the organism's exterior (the lumen of the gut, renal tubule and glandular duct), differs in many important respects from the basolateral plasma membrane that is apposed to the interior of the organism. The generation and maintenance of epithelial polarity require a highly specialized subcellular machinery to bring proteins to their appropriate sites of action. This is a dynamic process involving the interpretation of sorting signals, vectorial delivery mechanisms, membrane-specific fusion and retention processes. Here, w...
Source: Biology of the Cell - November 18, 2009 Category: Cytology Authors: Carmosino M, Valenti G, Caplan M, Svelto M Tags: Biol Cell Source Type: journals

The GIT/PIX complexes regulate the chemotactic response of rat basophilic leukemia cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. Our results show that endogenous GIT complexes are involved in the regulation of chemoattractant-induced cell motility and receptor trafficking, and support previous findings indicating an important function of the GIT complexes in the regulation of different G protein-coupled receptors. Our data also indicate that endogenous GIT1 and GIT2 regulate distinct subsets of agonist-induced responses, and suggest a possible functional link between the control of receptor trafficking and the regulation of cell motility by GIT proteins. (Source: Biology of the Cell)
Source: Biology of the Cell - November 13, 2009 Category: Cytology Authors: M Gavina, L Za, R Molteni, R Pardi, I de Curtis Source Type: journals

Members of the CIP4 family of proteins participate in the regulation of platelet-derived growth factor receptor-{beta}-dependent actin reorganization and migrationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions Our findings shows that CIP4-like proteins induced membrane tubulation downstream of Cdc42 and that they have important roles in PDGF-dependent actin reorganization and cell migration by regulating internalization and activity of the PDGFRβ. Moreover, the data suggest an important role for the CIP4-like proteins in the regulation of the activity of the PDGF β-receptor. (Source: Biology of the Cell)
Source: Biology of the Cell - November 12, 2009 Category: Cytology Authors: M Toguchi, N Richnau, A Ruusala, P Aspenström Source Type: journals

Modulation of tumorigenesis and estrogen receptor-{alpha} expression by cell culture condition in a stem cell-derived breast epithelial cell lineEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion. Estrogen responsive cell line with ERα and CD44+/CD24-/low expression can be derived from breast epithelial stem cells. The tumorigenicity and estrogen response of these cells could depend on cell culture condition. The findings of this study have implications in regard to the origins of 1) ERα-positive breast cancers, 2) CD44+/CD24-/low breast tumor stem cells and 3) metastatic ability of breast cancer. (Source: Biology of the Cell)
Source: Biology of the Cell - November 9, 2009 Category: Cytology Authors: K Wang, A Kao, C Chang, J Lee, C Chai, M Hou, C Liu, E Tsai Source Type: journals

TLRR (lrrc67) interacts with PP1 and is associated with a cytoskeletal complex in the testisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: We demonstrate here that TLRR interacts with PP1, particularly the testis specific isoform, PP1γ2. Immunoaffinity purification confirms that TLRR is associated with the spermatid cytoskeleton. In addition, proteins involved in protein stability are part of the TLRR complex. These results support our hypothesis that TLRR links signaling molecules to the spermatid cytoskeleton in order to regulate important substrates involved in spermatid transformation. The translocation of TLRR from manchette to the centrosome region suggests a possible role for this protein in tail formation. Our finding that TLRR is a...
Source: Biology of the Cell - November 4, 2009 Category: Cytology Authors: R Wang, A Kaul, A O Sperry Source Type: journals

Focal adhesion kinase-dependent regulation of adhesive force involves vinculin recruitment to focal adhesionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. We demonstrate that FAK reduces steady-state adhesion strength by modulating vinculin recruitment to focal adhesions. These findings provide insights into the role of FAK in mechanical interactions between a cell and the extracellular matrix. (Source: Biology of the Cell)
Source: Biology of the Cell - November 2, 2009 Category: Cytology Authors: D W Dumbauld, K E. Michael, S K Hanks, A J Garcia Source Type: journals

Role of lipid metabolism in hepatitis C virus assembly and entry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
HCV (hepatitis C virus) represents a major global health problem. A consistent body of evidence has been accumulating, suggesting a peculiar overlap between the HCV life cycle and lipid metabolism. This association becomes evident both for the clinical symptoms of HCV infection and the molecular mechanisms underlying the morphogenesis and entry process of this virus. The HCV core-lipid droplets association seems to be central to the HCV morphogenesis process. Moreover, the biogenesis pathway of very-low-density lipoproteins has been shown to be involved in HCV morphogenesis with MTP (microsomal triacylglycerol transfer...
Source: Biology of the Cell - October 29, 2009 Category: Cytology Authors: Popescu CI, Dubuisson J Tags: Biol Cell Source Type: journals

Adrenomedullin is expressed during rodent dental tissue development and promotes cell growth and mineralisationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: During oral and dental development ADM initially localises to epithelial tissue whilst during later stages is present in mineralised secreting cells, including odontoblasts. ADM may regulate proliferation and mineralisation processes during development whilst in adulthood, it may be important in maintaining dental tissue homeostasis. (Source: Biology of the Cell)
Source: Biology of the Cell - October 13, 2009 Category: Cytology Authors: D S Musson, J L McLachlan, A J Sloan, A J Smith, P R Cooper Source Type: journals

Precise and rapid mapping protocol for Correlative Light and Electron Microscopy of small invertebrate organismsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We believe that this technique will contribute to correlative studies in multicellular models, and will facilitate the time-demanding procedure of specimen preparation for any kind of TEM. (Source: Biology of the Cell)
Source: Biology of the Cell - September 17, 2009 Category: Cytology Authors: I Kolotuev, Y Schwab, M Labouesse Source Type: journals

Secretory phospholipase A promotes MMP-9-mediated cell death by degrading type I collagen via the Erk pathway at an early stage of chondrogenesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Taken together, our results suggest that elevated levels of sPLA2 secreted by wing bud mesenchymal cells promote type I collagen degradation by MMP-9 in a manner typical of receptor mediation and that these events lead to apoptotic cell death. (Source: Biology of the Cell)
Source: Biology of the Cell - September 17, 2009 Category: Cytology Authors: Y Choi, D Kim, O Bang, S Kang, E Jin Source Type: journals

Recent development of the mononuclear phagocyte system: in memory of Metchnikoff and Ehrlich on the 100th Anniversary of the 1908 Nobel Prize in Physiology or Medicine.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monocytes/macrophages are critical for both immunity and homoeostasis. They are the outposts of the immune system in detecting invading pathogens or foreign antigens for homoeostatic clearance and antigen processing for the initiation and effector stages of both innate and adaptive immunity. In addition, monocytes/macrophages often function as control switches for immune system balance between pro- and anti-inflammatory reactions. In the beginning of this article, I would like to briefly introduce the achievements of Metchnikoff and Ehrlich in immunology, including Metchnikoff's cell theory, since they have both greatl...
Source: Biology of the Cell - September 13, 2009 Category: Cytology Authors: Chang ZL Tags: Biol Cell Source Type: journals

Class I Rab11-Family Interacting Proteins are binding targets for the Rab14 GTPaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion The data presented here, which identifies the class I FIPs as the first putative effector proteins for the Rab14 GTPase, indicates greater complexity in the Rab-binding specificity of the class I FIP proteins. (Source: Biology of the Cell)
Source: Biology of the Cell - August 24, 2009 Category: Cytology Authors: E E. Kelly, C P. Horgan, C Adams, T M. Patzer, D M. Ní Shúilleabháin, J C. Norman, M W. McCaffrey Source Type: journals

The S-phase checkpoint: targeting the replication fork.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The S-phase checkpoint is a surveillance mechanism, mediated by the protein kinases Mec1 and Rad53 in the budding yeast Saccharomyces cerevisiae (ATR and Chk2 in human cells, respectively) that responds to DNA damage and replication perturbations by co-ordinating a global cellular response necessary to maintain genome integrity. A key aspect of this response is the stabilization of DNA replication forks, which is critical for cell survival. A defective checkpoint causes irreversible replication-fork collapse and leads to genomic instability, a hallmark of cancer cells. Although the precise mechanisms by which Mec1/Rad5...
Source: Biology of the Cell - August 20, 2009 Category: Cytology Authors: Segurado M, Tercero JA Tags: Biol Cell Source Type: journals

A novel protein, sperm head and tail associated protein (SHTAP) interacts with cysteine rich secretory protein 2 (CRISP2) during spermatogenesis in the mouseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. This study is the first report of the identification, annotation, and expression analysis of the mouse Shtap gene. The redistribution observed during sperm capacitation raises the possibility that SHTAP and the SHTAP-CRISP2 complex play a role in the attainment of sperm functional competence. (Source: Biology of the Cell)
Source: Biology of the Cell - August 17, 2009 Category: Cytology Authors: D Jamsai, S Rijal, D M Bianco, A E O’Connor, D J Merriner, S J Smith, G M Gibbs, M K O’Bryan Source Type: journals

Interleukin-1{beta} increases gap junctional communication among synovial fibroblasts via the extracellular signal regulated kinase pathwayEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. These data show that interleukin-1β acts through the extracellular signal regulated kinase signaling cascade to alter the expression and function of connexin43 in synovial fibroblasts. (Source: Biology of the Cell)
Source: Biology of the Cell - August 5, 2009 Category: Cytology Authors: C Niger, F D. Howell, J P. Stains Source Type: journals

Stem/progenitor cells in liver injury repair and regeneration.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Morbidity and mortality from cirrhosis is increasing rapidly in the world. Currently, orthotopic liver transplantation is the only definitive therapeutic option. However, its clinical use is limited, because of poor long-term graft survival, donor organ shortage and high costs associated with the procedure. Stem cell replacement strategies are therefore being investigated as an attractive alternative approach to liver repair and regeneration. In this review we discuss recent preclinical and clinical investigations that explore the therapeutic potential of stem cells in repair of liver injuries. Several types of stem ce...
Source: Biology of the Cell - August 1, 2009 Category: Cytology Authors: Zhao Q, Ren H, Zhu D, Han Z Tags: Biol Cell Source Type: journals

Plasma membrane and nuclear envelope integrity during the blebbing stage of apoptosis: a time-lapse studyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. These results show that the functional integrity of the nuclear envelope and plasma membrane may be conserved until the end of the execution phase of apoptosis. (Source: Biology of the Cell)
Source: Biology of the Cell - July 26, 2009 Category: Cytology Authors: R Andrade, L Crisol, R Prado, M D. Boyano, J Arluzea, J Aréchaga Source Type: journals

Newly-formed E-cadherin contacts do not activate Cdc42 or induce filopodia protrusion in human keratinocytesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: We conclude that Cdc42 does not participate in the early events that initiate stable cadherin adhesion in keratinocytes. Yet, it is feasible that Cdc42 may be activated at later time points or by other receptors. Cdc42 can then participate in additional functions during polarization such as Golgi re-positioning or baso-lateral trafficking. (Source: Biology of the Cell)
Source: Biology of the Cell - July 6, 2009 Category: Cytology Authors: J Carr Erasmus, S Aresta, S Nola, E Caron, V M.M. Braga Source Type: journals

The p24 family and selective transport processes at the ER-Golgi interface.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The secretory pathway is of vital importance for eukaryotic cells and has a pivotal role in the synthesis, sorting, processing and secretion of a large variety of bioactive molecules involved in intercellular communication. One of the key processes in the secretory pathway concerns the transport of cargo proteins from the ER (endoplasmic reticulum) to the Golgi. Type-I transmembrane proteins of approximately 24 kDa are abundantly present in the membranes of the early secretory pathway, and bind the COPI and COPII coat complexes that cover vesicles travelling between the membranes. These p24 proteins are thought to play...
Source: Biology of the Cell - July 3, 2009 Category: Cytology Authors: Strating JR, Martens GJ Tags: Biol Cell Source Type: journals

Signal transducer and activator of transcription 3 regulates adipocyte differentiation via peroxisome proliferator-activated receptor {gamma}Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. These data indicate that STAT3 functions as a critical factor for adipogenesis via a mechanism involving the PPARγ activation pathway. (Source: Biology of the Cell)
Source: Biology of the Cell - June 29, 2009 Category: Cytology Authors: D Wang, Y Zhou, W Lei, K Zhang, J Shi, Y Hu, G Shu, J Song Source Type: journals

Structure-function relationships in telomerase genes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The TERT (telomerase reverse transcriptase) subunit of telomerase is an intensively studied macromolecule due to its key importance in maintaining genome integrity and role in cellular aging and cancer. In an effort to provide an up-to-date overview of the topic, we discuss the structure of TERT genes, their alternative splicing products and their functions. Nucleotide databases contain more than 90 full-length cDNA sequences of telomerase protein subunits. Numerous in silico, in vitro and in vivo experimental techniques have revealed a great deal of structural and functional data describing particular features of the ...
Source: Biology of the Cell - June 27, 2009 Category: Cytology Authors: Sýkorová E, Fajkus J Tags: Biol Cell Source Type: journals

Integrin alpha8beta1 regulates adhesion, migration and proliferation of human intestinal crypt cells via a predominant RhoA/ROCK dependent mechanism.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Integrin alpha8beta1 is expressed in epithelial cells. In intestinal crypt cells, alpha8beta1 is closely involved in the regulation of adhesion, migration and cell proliferation via a predominant RhoA/ROCK dependent mechanism. These results suggest an important role for this integrin in intestinal crypt cell homeostasis. PMID: 19527220 [PubMed - as supplied by publisher] (Source: Biology of the Cell)
Source: Biology of the Cell - June 14, 2009 Category: Cytology Authors: Benoit YD, Lussier C, Ducharme PA, Sivret S, Schnapp LM, Basora N, Beaulieu JF Tags: Biol Cell Source Type: journals

Integrin {alpha}8{beta}1 regulates adhesion, migration and proliferation of human intestinal crypt cells via a predominant RhoA/ROCK dependent mechanismEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Integrin α8β1 is expressed in epithelial cells. In intestinal crypt cells, α8β1 is closely involved in the regulation of adhesion, migration and cell proliferation via a predominant RhoA/ROCK dependent mechanism. These results suggest an important role for this integrin in intestinal crypt cell homeostasis. (Source: Biology of the Cell)
Source: Biology of the Cell - June 14, 2009 Category: Cytology Authors: Y D. Benoit, C Lussier, P Ducharme, S Sivret, L M. Schnapp, N Basora, J Beaulieu Source Type: journals

Inhibiting Crm1 causes the formation of excess acentriolar spindle poles containing NuMA and B23, but does not affect centrosome numbers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Inhibition of Crm1 in early metaphase favors the formation of supplementary acentriolar spindle poles. B23 and NUMA are present at these poles that ultimately focus around the centrosome. Inhibition of Crm1 at metaphase has no effect on the control of centrosome numbers. PMID: 19522705 [PubMed - as supplied by publisher] (Source: Biology of the Cell)
Source: Biology of the Cell - June 11, 2009 Category: Cytology Authors: Rousselet A Tags: Biol Cell Source Type: journals

Inhibiting Crm1 causes the formation of excess acentriolar spindle poles containing NuMA and B23, but does not affect centrosome numbersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Inhibition of Crm1 in early metaphase favors the formation of supplementary acentriolar spindle poles. B23 and NUMA are present at these poles that ultimately focus around the centrosome. Inhibition of Crm1 at metaphase has no effect on the control of centrosome numbers. (Source: Biology of the Cell)
Source: Biology of the Cell - June 11, 2009 Category: Cytology Authors: A Rousselet Source Type: journals

Altering PI3K-Akt signaling in zebrafish embryos affects Pten phosphorylation and gastrulation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. These data suggest that Ptena454 regulation is correlated to changes in phospho-Akt levels. We propose a model by which homeostasis in rapidly dividing and migrating embryonic cells depends on a counterbalance between pro-survival signaling employing CK2 and Gsk-3 and the pro-apoptotic activity of Ptena454. PMID: 19515017 [PubMed - as supplied by publisher] (Source: Biology of the Cell)
Source: Biology of the Cell - June 9, 2009 Category: Cytology Authors: Finkielsztein A, Kelly GM Tags: Biol Cell Source Type: journals

Altering PI3K-Akt signaling in zebrafish embryos affects Pten phosphorylation and gastrulation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. These data suggest that Ptena454 regulation is correlated to changes in phospho-Akt levels. We propose a model by which homeostasis in rapidly dividing and migrating embryonic cells depends on a counterbalance between pro-survival signaling employing CK2 and Gsk-3 and the pro-apoptotic activity of Ptena454. (Source: Biology of the Cell)
Source: Biology of the Cell - June 9, 2009 Category: Cytology Authors: A Finkielsztein, G M Kelly Source Type: journals

Wnt5 is required for notochord cell intercalation in the ascidian Halocynthia roretziEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion. The present study highlights the role of Wnt5α signal in notochord convergent extension movements in ascidian embryos. Our results raise the novel possibility that Wnt5α functions in a cell-autonomous manner in activation of the Wnt/PCP pathway to polarize the protrusive activity that drives convergent extension. (Source: Biology of the Cell)
Source: Biology of the Cell - June 8, 2009 Category: Cytology Authors: T Niwano, N Takatori, G Kumano, H Nishida Source Type: journals

Wnt5 is required for notochord cell intercalation in the ascidian Halocynthia roretzi.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion. The present study highlights the role of Wnt5alpha signal in notochord convergent extension movements in ascidian embryos. Our results raise the novel possibility that Wnt5alpha functions in a cell-autonomous manner in activation of the Wnt/PCP pathway to polarize the protrusive activity that drives convergent extension. PMID: 19505288 [PubMed - as supplied by publisher] (Source: Biology of the Cell)
Source: Biology of the Cell - June 7, 2009 Category: Cytology Authors: Niwano T, Takatori N, Kumano G, Nishida H Tags: Biol Cell Source Type: journals

The tumour suppressor Pdcd4: recent advances in the elucidation of function and regulation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pdcd4 (programmed cell death 4) has been known as a tumour suppressor gene and potential target for anticancer therapies for several years. Initially, Pdcd4 was identified as a gene that is up-regulated during apoptosis, but its precise role still remains to be defined. However, there is increasing evidence that Pdcd4 levels influence transcription, as well as translation, modulate different signal transduction pathways and might act as a tumour suppressor. Interestingly, recent data suggest that Pdcd4 function may depend on cell type and/or genetic background. This review summarizes the current knowledge regarding the...
Source: Biology of the Cell - May 31, 2009 Category: Cytology Authors: Lankat-Buttgereit B, Göke R Tags: Biol Cell Source Type: journals

Production of reactive oxygen species, impairment of photosynthetic function and dynamic changes of mitochondria are early events in cadmium induced cell death in Arabidopsis thalianaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The effects of Cd on the morphology and function of the chloroplast and mitochondria, as well as on the production and localization of reactive oxygen species (ROS) were studied at the single-cell level in Arabidopsis. Results demonstrated that the morphology of chloroplast changed after Cd treatment, and photochemical efficiency dramatically declined prior to obvious morphological distortion in the chloroplast. A quick burst of ROS was detected after Cd treatment. The ROS appeared first in the mitochondria and subsequently in the chloroplast. Simultaneously, the mitochondria clumped irregularly around the chloroplasts or ...
Source: Biology of the Cell - May 19, 2009 Category: Cytology Authors: Y Bi, W Chen, W Zhang, Q Zhou, L Yuan, D Xing Source Type: journals

Production of reactive oxygen species, impairment of photosynthetic function and dynamic changes of mitochondria are early events in cadmium induced cell death in Arabidopsis thaliana.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The effects of Cd on the morphology and function of the chloroplast and mitochondria, as well as on the production and localization of reactive oxygen species (ROS) were studied at the single-cell level in Arabidopsis. Results demonstrated that the morphology of chloroplast changed after Cd treatment, and photochemical efficiency dramatically declined prior to obvious morphological distortion in the chloroplast. A quick burst of ROS was detected after Cd treatment. The ROS appeared first in the mitochondria and subsequently in the chloroplast. Simultaneously, the mitochondria clumped irregularly around the chloroplasts...
Source: Biology of the Cell - May 18, 2009 Category: Cytology Authors: Bi Y, Chen W, Zhang W, Zhou Q, Yuan L, Xing D Tags: Biol Cell Source Type: journals

The ESCRT-III protein CeVPS-32 is enriched in domains distinct from CeVPS-27 and CeVPS-23 at the endosomal membrane of epithelial cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Within the endocytic pathway, the Endosomal Sorting Complex Required for Transport (ESCRT) machinery is essential for the biogenesis of MultiVesicular Bodies (MVB). In yeast, ESCRTs are recruited at the endosomal membrane and involved in cargo sorting into intralumenal vesicles of the MVB. Here, we characterize the ESCRT-III protein CeVPS-32 in the nematode C. elegans and its interactions with CeVPS-27, CeVPS-23 and CeVPS-4. In contrast to other CevpsE genes, depletion of Cevps-32 is embryonic lethal with severe defects in the remodelling of epithelial cell shape during organogenesis. Furthermore, Cevps-32 animals display ...
Source: Biology of the Cell - May 12, 2009 Category: Cytology Authors: X Michelet, A Alberti, L Benkemoun, N Roudier, C Lefebvre, R Legouis Source Type: journals

The ESCRT-III protein CeVPS-32 is enriched in domains distinct from CeVPS-27 and CeVPS-23 at the endosomal membrane of epithelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Within the endocytic pathway, the Endosomal Sorting Complex Required for Transport (ESCRT) machinery is essential for the biogenesis of MultiVesicular Bodies (MVB). In yeast, ESCRTs are recruited at the endosomal membrane and involved in cargo sorting into intralumenal vesicles of the MVB. Here, we characterize the ESCRT-III protein CeVPS-32 in the nematode C. elegans and its interactions with CeVPS-27, CeVPS-23 and CeVPS-4. In contrast to other CevpsE genes, depletion of Cevps-32 is embryonic lethal with severe defects in the remodelling of epithelial cell shape during organogenesis. Furthermore, Cevps-32 animals disp...
Source: Biology of the Cell - May 11, 2009 Category: Cytology Authors: Michelet X, Alberti A, Benkemoun L, Roudier N, Lefebvre C, Legouis R Tags: Biol Cell Source Type: journals

Cell cycle analysis of fetal germ cells during sex differentiation in miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This study has provided a detailed analysis of cell cycle gene expression during fetal germ cell development and identified candidate factors warranting further investigation in order to understand cases of aberrant cell cycle control in these specialised cells. (Source: Biology of the Cell)
Source: Biology of the Cell - May 6, 2009 Category: Cytology Authors: C Spiller, D Wilhelm, P Koopman Source Type: journals

Cell cycle analysis of fetal germ cells during sex differentiation in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This study has provided a detailed analysis of cell cycle gene expression during fetal germ cell development and identified candidate factors warranting further investigation in order to understand cases of aberrant cell cycle control in these specialised cells. PMID: 19419345 [PubMed - as supplied by publisher] (Source: Biology of the Cell)
Source: Biology of the Cell - May 5, 2009 Category: Cytology Authors: Spiller C, Wilhelm D, Koopman P Tags: Biol Cell Source Type: journals

Distribution, pharmacological characterization and function of the translocator protein (18kDa) in rat small intestineEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The functional differences in subpopulations of TSPO in different regions of the intestine could be related to structural organization of mitochondrial protein complexes that mediate the ability of TSPO to modulate either chloride secretion or absorption in the duodenum and ileum, respectively. (Source: Biology of the Cell)
Source: Biology of the Cell - April 24, 2009 Category: Cytology Authors: M Anibal Ostuni, G Péranzi, R A. Ducroc, M Fasseu, B Vidic, J Dumont, V Papadopoulos, J Lacapere Source Type: journals

Emerin-prelamin A interplay in human fibroblastsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion. These results indicate that emerin-prelamin A interplay influences nuclear organization. This finding may be relevant to the understanding of laminopathies. (Source: Biology of the Cell)
Source: Biology of the Cell - March 26, 2009 Category: Cytology Authors: C Capanni, R Del Coco, E Mattioli, D Camozzi, M Columbaro, E Schena, L Merlini, S Squarzoni, N Mario Maraldi, G Lattanzi Source Type: journals

Role of 5'- and 3'-untranslated regions of mRNAs in human diseases.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Protein synthesis is often regulated at the level of initiation of translation, making it a critical step. This regulation occurs by both the cis-regulatory elements, which are located in the 5'- and 3'-UTRs (untranslated regions), and trans-acting factors. A breakdown in this regulation machinery can perturb cellular metabolism, leading to various physiological abnormalities. The highly structured UTRs, along with features such as GC-richness, upstream open reading frames and internal ribosome entry sites, significantly influence the rate of translation of mRNAs. In this review, we discuss how changes in the cis-regul...
Source: Biology of the Cell - March 14, 2009 Category: Cytology Authors: Chatterjee S, Pal JK Tags: Biol Cell Source Type: journals

Cross-talk between the VEGF-A and HGF signalling pathways in endothelial cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. Under angiogenic conditions, combining VEGF-A with HGF can promote neovascularisation by enhancing intracellular signalling and allowing more finely regulated control of the signalling molecules involved in the regulation of the cytoskeleton and cellular migration and morphogenesis. (Source: Biology of the Cell)
Source: Biology of the Cell - March 12, 2009 Category: Cytology Authors: E Sulpice, S Ding, B Muscatelli-Groux, M Bergé, Z Chao Han, J Plouët, G Tobelem, T Merkulova-Rainon Source Type: journals

Two new miR-16 targets: Caprin-1 and HMGA1, proteins implicated in cell proliferationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Taken together, our data demonstrated that miR-16 can negatively regulate the two new targets HMGA1 and caprin-1 involved in cell proliferation. In addition, we also showed that the inhibition of cyclin E expression was due, at least in part, to a decrease in its mRNA stability. (Source: Biology of the Cell)
Source: Biology of the Cell - February 27, 2009 Category: Cytology Authors: T Kaddar, J Rouault, W Chien, A Chebel, M Gadoux, G Salles, M Ffrench, J Magaud Source Type: journals

Ins(1,4,5)P3 receptor type 1 associates with AKAP9 (AKAP450 variant) and protein kinase A type II{beta} in the Golgi apparatus in cerebellar granule cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. Taken together these results suggest that InsP3R1 forms a complex with AKAP450 and PKAIIβ localised in the Golgi apparatus of cerebellar granule cells. By contrast, the association of InsP3R1 with PKA in Purkinje cells would require a different macromolecular complex. (Source: Biology of the Cell)
Source: Biology of the Cell - February 24, 2009 Category: Cytology Authors: M Collado-Hilly, J Coquil Source Type: journals

Progesterone induces nano-scale molecular modifications on endometrial epithelial cell surfacesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions – Progesterone treatment induces changes to the cell surface as a result of nanoscale molecular modifications in response to external hormonal treatments. AFM provides the basis for the identification, visualisation and quantification of these cell surface nanoscale changes. Together these findings demonstrate the utility of AFM for use in reproductive science and cancer biology where it could be applied in both in vitro analysis of protein structure-function relationships and clinical diagnosis. (Source: Biology of the Cell)
Source: Biology of the Cell - February 24, 2009 Category: Cytology Authors: L W Francis, P D Lewis, D Gonzalez, T Ryder, G Webb, L A Joels, J O White, C W Wright, R S Conlan Source Type: journals

Histone deacetylases: salesmen and customers in the post-translational modification market.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
HDACs (histone deacetylases) are enzymes that remove the acetyl moiety from N-epsilon-acetylated lysine residues in histones and non-histone proteins. In recent years, it has turned out that HDACs themselves are also subject to post-translational modification. Such structural alterations can determine the stability, localization, activity and protein-protein interactions of HDACs. This subsequently affects the modification of their substrates and the co-ordination of cellular signalling networks. Intriguingly, physiologically relevant non-histone proteins are increasingly found to be deacetylated by HDACs, and aberrant...
Source: Biology of the Cell - February 13, 2009 Category: Cytology Authors: Brandl A, Heinzel T, Krämer OH Tags: Biol Cell Source Type: journals

Spatiotemporal activation of caspase-dependent and -independent pathways in staurosporine-induced apoptosis of p53wt and p53mt human cervical carcinoma cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. Our data highlight that staurosporine engages the intrinsic mitochondrial apoptotic pathway via caspase-8 or caspase-9 signalling cascades and via caspase-independent cell death, as well as through p53 activity. (Source: Biology of the Cell)
Source: Biology of the Cell - February 13, 2009 Category: Cytology Authors: M Nicolier, A Decrion-Barthod, S Launay, J Prétet, C Mougin Source Type: journals

The ribonome: a dominant force in co-ordinating gene expression.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The ribonome is the total cellular complement of RNAs and their regulatory factors functioning dynamically in time and space within ribonucleoprotein complexes. We theorize that the ribonome is an ancient central co-ordinator that has evolved to communicate on multiple levels to the proteome on the one hand (feed-forward), and the transcriptome and RNA processing machinery on the other (feed-back). Furthermore, the ribonome can potentially communicate to other cells horizontally with implications for biological information transfer and for the evolution of both RNA and DNA operating systems. The post-transcriptional RN...
Source: Biology of the Cell - January 22, 2009 Category: Cytology Authors: Mansfield KD, Keene JD Tags: Biol Cell Source Type: journals

The function of ROCO proteins in health and disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The ROCO family of multidomain proteins extends across the eukaryotes, and has been implicated in numerous cellular processes. Following the description of mutations causing PD (Parkinson's disease) in a human representative of the ROCO family, LRRK2 (leucine-rich repeat kinase 2), a great deal of research has been carried out into these proteins. This review examines the published data regarding the roles the ROCO proteins are thought to play in cell processes, and how the structure and domain organization of these proteins relates to their function. PMID: 19152505 [PubMed - in process] (Source: Biology of the Cell)
Source: Biology of the Cell - January 22, 2009 Category: Cytology Authors: Lewis PA Tags: Biol Cell Source Type: journals

Evidence for cross-reactivity of JAM-C antibodies: Implications for cellular localization studiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. These results demonstrate that a majority of available anti-human JAM-C antibodies cross-react with phosphorylated K8 and suggest that cellular localization studies using these reagents should be interpreted with caution. Of the JAM-C antibodies tested, only mAb PACA4 is monospecific for human JAM-C. Analyses using PACA4 reveal that JAM-C expression is variable in different epithelial cell lines with co-localization at TJs. (Source: Biology of the Cell)
Source: Biology of the Cell - January 14, 2009 Category: Cytology Authors: A Betanzos, M Schnoor, E A Severson, T W Liang, C A Parkos Source Type: journals

GFP-targeting allows visualisation of the apicoplast throughout the life cycle of live malaria parasitesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we have generated P. berghei parasites in which GFP is targeted to the apicoplast using the specific targeting sequence of acyl carrier protein (ACP), which has allowed us to visualise this organelle in live Plasmodium parasites. During each phase of asexual reproduction, the apicoplast becomes highly branched, but remains as a single organelle until the completion of nuclear division, whereupon it divides and is rapidly segregated into newly forming daughter cells. We have shown that the antimicrobial agents azithromycin, clindamycin and doxycycline block development of the apicoplast during exo-erythrocyti...
Source: Biology of the Cell - January 14, 2009 Category: Cytology Authors: R R Stanway, T Witt, B Zobiak, M Aepfelbacher, V T. Heussler Source Type: journals