Kinetic Study of the Esterase-like Activity of Albumin following Condensation by Macromolecular Crowding
This article investigates the kinetic profile of para-nitrophenol acetate hydrolysis by BSA in its condensed state with poly(ethylene) glycol (PEG) as the crowding agent. Furthermore, the initial BSA concentration was varied between 0.25 and 1 mM which allowed us to modify the size distribution, the volume fraction, and the partition coefficient (varying from 136 to 180). Hence, the amount of BSA originally added was a simple way to modulate the size and density of the condensates. Compared with dilute BSA, the initial velocity (vi) with condensates was dramatically reduced. From the Michaelis-Menten fits, the extracted Mi...
Source: Biomacromolecules - April 17, 2024 Category: Biochemistry Authors: Honorine Lamy Erika Bullier-Marchandin Cl éo Pointel Aline Echalard Guy Daniel Ladam Ga ëtan Lutzweiler Source Type: research
Modular Synthesis of PEG-Dendritic Block Copolymers by Thermal Azide-Alkyne Cycloaddition with Internal Alkynes and Evaluation of their Self-Assembly for Drug Delivery Applications
Biomacromolecules. 2024 Apr 13. doi: 10.1021/acs.biomac.3c01429. Online ahead of print.ABSTRACTLinear-dendritic block copolymers assemble in solution due to differences in the solubility or charge properties of the blocks. The monodispersity and multivalency of the dendritic block provide unparalleled control for the design of drug delivery systems when incorporating poly(ethylene glycol) (PEG) as a linear block. An accelerated synthesis of PEG-dendritic block copolymers based on the click and green chemistry pillars is described. The tandem composed of the thermal azide-alkyne cycloaddition with internal alkynes and azide...
Source: Biomacromolecules - April 13, 2024 Category: Biochemistry Authors: Samuel Parcero-Bouzas Juan Correa Celia Jimenez-Lopez Bruno Delgado Gonzalez Eduardo Fernandez-Megia Source Type: research
Modular Synthesis of PEG-Dendritic Block Copolymers by Thermal Azide-Alkyne Cycloaddition with Internal Alkynes and Evaluation of their Self-Assembly for Drug Delivery Applications
Biomacromolecules. 2024 Apr 13. doi: 10.1021/acs.biomac.3c01429. Online ahead of print.ABSTRACTLinear-dendritic block copolymers assemble in solution due to differences in the solubility or charge properties of the blocks. The monodispersity and multivalency of the dendritic block provide unparalleled control for the design of drug delivery systems when incorporating poly(ethylene glycol) (PEG) as a linear block. An accelerated synthesis of PEG-dendritic block copolymers based on the click and green chemistry pillars is described. The tandem composed of the thermal azide-alkyne cycloaddition with internal alkynes and azide...
Source: Biomacromolecules - April 13, 2024 Category: Biochemistry Authors: Samuel Parcero-Bouzas Juan Correa Celia Jimenez-Lopez Bruno Delgado Gonzalez Eduardo Fernandez-Megia Source Type: research
Modular Synthesis of PEG-Dendritic Block Copolymers by Thermal Azide-Alkyne Cycloaddition with Internal Alkynes and Evaluation of their Self-Assembly for Drug Delivery Applications
Biomacromolecules. 2024 Apr 13. doi: 10.1021/acs.biomac.3c01429. Online ahead of print.ABSTRACTLinear-dendritic block copolymers assemble in solution due to differences in the solubility or charge properties of the blocks. The monodispersity and multivalency of the dendritic block provide unparalleled control for the design of drug delivery systems when incorporating poly(ethylene glycol) (PEG) as a linear block. An accelerated synthesis of PEG-dendritic block copolymers based on the click and green chemistry pillars is described. The tandem composed of the thermal azide-alkyne cycloaddition with internal alkynes and azide...
Source: Biomacromolecules - April 13, 2024 Category: Biochemistry Authors: Samuel Parcero-Bouzas Juan Correa Celia Jimenez-Lopez Bruno Delgado Gonzalez Eduardo Fernandez-Megia Source Type: research
Mussel-Mimetic Hydrogel Coating with Anticoagulant and Antiinflammatory Properties on a Poly(lactic acid) Vascular Stent
In this study, we developed a multicross-linking hydrogel on the polylactic acid substrates by dip coating that boasts impressive antithrombotic ability, antibacterial capability, mechanical stability, and self-healing ability. Gelatin methacryloyl, carboxymethyl chitosan, and oxidized sodium alginate construct a double-cross-linking hydrogel through the dynamic Schiff base chemical and in situ blue initiation reaction. Inspired by the adhesion mechanism employed by mussels, a triple-cross-linked hydrogel is formed with the addition of tannic acid to increase the adhesion and antibiofouling properties. The strength and hyd...
Source: Biomacromolecules - April 12, 2024 Category: Biochemistry Authors: Biwei Qiu Qianqian Cheng Rukun Chen Chunling Liu Jinchao Qin Qixia Jiang Source Type: research
Advanced Smart Biomaterials for Regenerative Medicine and Drug Delivery Based on Phosphoramidite Chemistry: From Oligonucleotides to Precision Polymers
Biomacromolecules. 2024 Apr 12. doi: 10.1021/acs.biomac.4c00259. Online ahead of print.ABSTRACTOver decades of development, while phosphoramidite chemistry has been known as the leading method in commercial synthesis of oligonucleotides, it has also revolutionized the fabrication of sequence-defined polymers (SDPs), offering novel functional materials in polymer science and clinical medicine. This review has introduced the evolution of phosphoramidite chemistry, emphasizing its development from the synthesis of oligonucleotides to the creation of universal SDPs, which have unlocked the potential for designing programmable ...
Source: Biomacromolecules - April 12, 2024 Category: Biochemistry Authors: Bo Yang Ting Cui Liang Guo Lianqiang Dong Jun Wu Yongzheng Xing Yun Xu Jian Chen Yufei Wang Zhonghui Cui Yuanchen Dong Source Type: research
Mussel-Mimetic Hydrogel Coating with Anticoagulant and Antiinflammatory Properties on a Poly(lactic acid) Vascular Stent
In this study, we developed a multicross-linking hydrogel on the polylactic acid substrates by dip coating that boasts impressive antithrombotic ability, antibacterial capability, mechanical stability, and self-healing ability. Gelatin methacryloyl, carboxymethyl chitosan, and oxidized sodium alginate construct a double-cross-linking hydrogel through the dynamic Schiff base chemical and in situ blue initiation reaction. Inspired by the adhesion mechanism employed by mussels, a triple-cross-linked hydrogel is formed with the addition of tannic acid to increase the adhesion and antibiofouling properties. The strength and hyd...
Source: Biomacromolecules - April 12, 2024 Category: Biochemistry Authors: Biwei Qiu Qianqian Cheng Rukun Chen Chunling Liu Jinchao Qin Qixia Jiang Source Type: research
Advanced Smart Biomaterials for Regenerative Medicine and Drug Delivery Based on Phosphoramidite Chemistry: From Oligonucleotides to Precision Polymers
Biomacromolecules. 2024 Apr 12. doi: 10.1021/acs.biomac.4c00259. Online ahead of print.ABSTRACTOver decades of development, while phosphoramidite chemistry has been known as the leading method in commercial synthesis of oligonucleotides, it has also revolutionized the fabrication of sequence-defined polymers (SDPs), offering novel functional materials in polymer science and clinical medicine. This review has introduced the evolution of phosphoramidite chemistry, emphasizing its development from the synthesis of oligonucleotides to the creation of universal SDPs, which have unlocked the potential for designing programmable ...
Source: Biomacromolecules - April 12, 2024 Category: Biochemistry Authors: Bo Yang Ting Cui Liang Guo Lianqiang Dong Jun Wu Yongzheng Xing Yun Xu Jian Chen Yufei Wang Zhonghui Cui Yuanchen Dong Source Type: research
Enhanced Hydrogen Bonding by Urea Functionalization Tunes the Stability and Biological Properties of Peptide Amphiphiles
Biomacromolecules. 2024 Apr 11. doi: 10.1021/acs.biomac.3c01463. Online ahead of print.ABSTRACTSelf-assembled nanostructures such as those formed by peptide amphiphiles (PAs) are of great interest in biological and pharmacological applications. Herein, a simple and widely applicable chemical modification, a urea motif, was included in the PA's molecular structure to stabilize the nanostructures by virtue of intermolecular hydrogen bonds. Since the amino acid residue nearest to the lipid tail is the most relevant for stability, we decided to include the urea modification at that position. We prepared four groups of molecule...
Source: Biomacromolecules - April 11, 2024 Category: Biochemistry Authors: Huihua Xing Caleb Wigham Sieun Ruth Lee Aramis J Pereira Luana J de Campos Agust ín S Picco Cristi án Huck-Iriart Carlos Escudero Laura Perez-Chirinos Sandun Gajaweera Jeffrey Comer Ivan R Sasselli Samuel I Stupp R Helen Zha Martin Conda-Sheridan Source Type: research
Enzymatic Synthesis of Copolyesters with the Heteroaromatic Diol 3,4-Bis(hydroxymethyl)furan and Isomeric Dimethyl Furandicarboxylate Substitutions
This study explores the Candida antarctica lipase B (CALB)-catalyzed copolymerization of 3,4-BHMF with furan dicarboxylate isomers and aliphatic diols. The furanic copolyesters (co-FPEs) with higher polymerization degrees are obtained using 2,4-isomer, indicating CALB's preference. Material analysis revealed semicrystalline properties in all synthesized 2,5-FDCA-based co-FPEs, with multiple melting temperatures (Tm) from 53 to 124 °C and a glass-transition temperature (Tg) of 9-10 °C. 2,4-FDCA-based co-FPEs showed multiple Tm from 43 to 61 °C and Tg of -14 to 12 °C; one of them was amorphous. In addition, all co-FPEs s...
Source: Biomacromolecules - April 11, 2024 Category: Biochemistry Authors: Fitrilia Silvianti Dina Maniar Beatriz Agostinho Tijn C de Leeuw Albert Jan Jacob Woortman Jur van Dijken Shanmugam Thiyagarajan Andreia F Sousa Katja Loos Source Type: research
Enhanced Hydrogen Bonding by Urea Functionalization Tunes the Stability and Biological Properties of Peptide Amphiphiles
Biomacromolecules. 2024 Apr 11. doi: 10.1021/acs.biomac.3c01463. Online ahead of print.ABSTRACTSelf-assembled nanostructures such as those formed by peptide amphiphiles (PAs) are of great interest in biological and pharmacological applications. Herein, a simple and widely applicable chemical modification, a urea motif, was included in the PA's molecular structure to stabilize the nanostructures by virtue of intermolecular hydrogen bonds. Since the amino acid residue nearest to the lipid tail is the most relevant for stability, we decided to include the urea modification at that position. We prepared four groups of molecule...
Source: Biomacromolecules - April 11, 2024 Category: Biochemistry Authors: Huihua Xing Caleb Wigham Sieun Ruth Lee Aramis J Pereira Luana J de Campos Agust ín S Picco Cristi án Huck-Iriart Carlos Escudero Laura Perez-Chirinos Sandun Gajaweera Jeffrey Comer Ivan R Sasselli Samuel I Stupp R Helen Zha Martin Conda-Sheridan Source Type: research
Enzymatic Synthesis of Copolyesters with the Heteroaromatic Diol 3,4-Bis(hydroxymethyl)furan and Isomeric Dimethyl Furandicarboxylate Substitutions
This study explores the Candida antarctica lipase B (CALB)-catalyzed copolymerization of 3,4-BHMF with furan dicarboxylate isomers and aliphatic diols. The furanic copolyesters (co-FPEs) with higher polymerization degrees are obtained using 2,4-isomer, indicating CALB's preference. Material analysis revealed semicrystalline properties in all synthesized 2,5-FDCA-based co-FPEs, with multiple melting temperatures (Tm) from 53 to 124 °C and a glass-transition temperature (Tg) of 9-10 °C. 2,4-FDCA-based co-FPEs showed multiple Tm from 43 to 61 °C and Tg of -14 to 12 °C; one of them was amorphous. In addition, all co-FPEs s...
Source: Biomacromolecules - April 11, 2024 Category: Biochemistry Authors: Fitrilia Silvianti Dina Maniar Beatriz Agostinho Tijn C de Leeuw Albert Jan Jacob Woortman Jur van Dijken Shanmugam Thiyagarajan Andreia F Sousa Katja Loos Source Type: research
Enhancing Protein Solubility via Glycosylation: From Chemical Synthesis to Machine Learning Predictions
This study aimed to bridge this gap by investigating the solubility of a model glycoprotein molecule, the carbohydrate-binding module (CBM), through a two-stage process. In the first stage, an approach involving chemical synthesis, comparative analysis, and molecular dynamics simulations of a library of glycoforms was employed to elucidate the effect of different glycosylation patterns on solubility and the key factors responsible for the effect. In the second stage, a predictive mathematical formula, innovatively harnessing machine learning algorithms, was derived to relate solubility to the identified key factors and acc...
Source: Biomacromolecules - April 10, 2024 Category: Biochemistry Authors: Bo Ma Hedi Chen Jinyuan Gong Wenqiang Liu Xiuli Wei Yajing Zhang Xin Li Meng Li Yani Wang Shiying Shang Boxue Tian Yaohao Li Ruihan Wang Zhongping Tan Source Type: research
In Situ Synthesis and Self-Assembly of Peptide-PEG Conjugates: A Facile Method for the Construction of Fibrous Hydrogels
In this study, we present the synthesis of novel peptide-PEG conjugates and explore their comprehensive hydrogel properties. The hydrogel comprises double networks, with the first network formed through the self-assembly of peptides to create a β-sheet secondary structure. The second network is established through covalent bond formation via N-hydroxysuccinimide chemistry between peptides and a 4-arm PEG to form a covalently linked network. Importantly, our findings reveal that this hydrogel formation method can be applied to other peptides containing lysine-rich sequences. Upon encapsulation of the hydrogel with antimicr...
Source: Biomacromolecules - April 10, 2024 Category: Biochemistry Authors: Haritha Asokan-Sheeja Kamal Awad Jiazhu Xu Myan Le Jenny N Nguyen Na Nguyen Tam P Nguyen Kytai T Nguyen Yi Hong Venu G Varanasi Xiaohua Liu He Dong Source Type: research
Enhancing Protein Solubility via Glycosylation: From Chemical Synthesis to Machine Learning Predictions
This study aimed to bridge this gap by investigating the solubility of a model glycoprotein molecule, the carbohydrate-binding module (CBM), through a two-stage process. In the first stage, an approach involving chemical synthesis, comparative analysis, and molecular dynamics simulations of a library of glycoforms was employed to elucidate the effect of different glycosylation patterns on solubility and the key factors responsible for the effect. In the second stage, a predictive mathematical formula, innovatively harnessing machine learning algorithms, was derived to relate solubility to the identified key factors and acc...
Source: Biomacromolecules - April 10, 2024 Category: Biochemistry Authors: Bo Ma Hedi Chen Jinyuan Gong Wenqiang Liu Xiuli Wei Yajing Zhang Xin Li Meng Li Yani Wang Shiying Shang Boxue Tian Yaohao Li Ruihan Wang Zhongping Tan Source Type: research
