Fabrication and surface modification of poly lactic acid (PLA) scaffolds with epidermal growth factor for neural tissue engineering.
Authors: Haddad T, Noel S, Liberelle B, El Ayoubi R, Ajji A, De Crescenzo G
Abstract
In an effort to design biomaterials that may promote repair of the central nervous system, 3-dimensional scaffolds made of electrospun poly lactic acid nanofibers with interconnected pores were fabricated. These scaffolds were functionalized with polyallylamine to introduce amine groups by wet chemistry. Experimental conditions of the amination protocol were thoroughly studied and selected to introduce a high amount of amine group while preserving the mechanical and structural properties of the scaffold. Subsequent covalen...
Source: Biomatter - October 17, 2016 Category: Biotechnology Tags: Biomatter Source Type: research
Enhanced bioactivity of glass ionomer cement by incorporating calcium silicates.
Authors: Chen S, Cai Y, Engqvist H, Xia W
Abstract
Glass ionomer cements (GIC) are known as a non-bioactive dental cement. During setting the GIC have an acidic pH, driven by the acrylic acid component. It is a challenge to make GIC alkaline without disturbing its mechanical properties. One strategy was to add slowly reacting systems with an alkaline pH. The aim of the present study is to investigate the possibility of forming a bioactive dental material based on the combination of glass ionomer cement and calcium silicates. Two types of GIC were used as control. Wollastonite (CS also denoted β-CaSiO3) or...
Source: Biomatter - January 21, 2016 Category: Biotechnology Tags: Biomatter Source Type: research
The effect of oligo(trimethylene carbonate) addition on the stiffness of acrylic bone cement.
Authors: Persson C, López A, Fathali H, Hoess A, Rojas R, Ott MK, Hilborn J, Engqvist H
Abstract
With the increasing elderly population an increase in the number of bony fractures associated to age-related diseases such as osteoporosisalso follows. The relatively high stiffness of the acrylic bone cements used in these patients has been suggested to give raise to a suboptimal load distribution surrounding the cement in vivo, and hence contribute to clinical complications, such as additional fractures. The aim of this study was to develop a low-modulus bone cement, based on currently used, commercially ava...
Source: Biomatter - January 16, 2016 Category: Biotechnology Tags: Biomatter Source Type: research
Corrigendum.
Authors:
PMID: 26594851 [PubMed - in process] (Source: Biomatter)
Source: Biomatter - November 27, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
Knitted polylactide 96/4 L/D structures and scaffolds for tissue engineering: shelf life, in vitro and in vivo studies.
This study covers the whole production cycle, from biodegradable polymer processing to an in vivo tissue engineered construct. Six different biodegradable polylactide 96/4 L/D single jersey knits were manufactured using either four or eight multifilament fiber batches. The properties of those were studied in vitro for 42 weeks and in 0- to 3-year shelf life studies. Three types (Ø 12, 15 and 19 mm) of cylindrical scaffolds were manufactured from the knit, and the properties of those were studied in vitro for 48 weeks. For the Ø 15 mm scaffold type, mechanical properties were also studied in a one-year in vivo experiment....
Source: Biomatter - November 20, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
Synthesis and characterization of chitosan-polyvinyl alcohol-bioactive glass hybrid membranes.
Authors: Dias LL, Mansur HS, Donnici CL, Pereira MM
Abstract
The tissue engineering strategy is a new approach for the regeneration of cementum, which is essential for the regeneration of the periodontal tissue. This strategy involves the cell cultures present in this tissue, called cementoblasts, and located on an appropriate substrate for posterior implantation in the regeneration site. Prior studies from our research group have shown that the proliferation and viability of cementoblasts increase in the presence of the ionic dissolution products of bioactive glass particles. Therefore, one possible appro...
Source: Biomatter - November 20, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
Calcium orthophosphates: occurrence, properties, biomineralization, pathological calcification and biomimetic applications.
Authors: Dorozhkin SV
Abstract
The present overview is intended to point the readers' attention to the important subject of calcium orthophosphates. This type of materials is of special significance for human beings, because they represent the inorganic part of major normal (bones, teeth and antlers) and pathological (i.e., those appearing due to various diseases) calcified tissues of mammals. For example, atherosclerosis results in blood vessel blockage caused by a solid composite of cholesterol with calcium orthophosphates, while dental caries and osteoporosis mean a partial decalcification of teeth and ...
Source: Biomatter - November 20, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
Effects of a synthetic bioactive peptide on neurite growth and nerve growth factor release in chondroitin sulfate hydrogels.
Authors: Conovaloff AW, Beier BL, Irazoqui PP, Panitch A
Abstract
Previous work has revealed robust dorsal root ganglia neurite growth in hydrogels of chondroitin sulfate. In the current work, it was determined whether addition of a synthetic bioactive peptide could augment neurite growth in these matrices via enhanced binding and sequestering of growth factors. Fluorescence recovery after photobleaching studies revealed that addition of peptide slowed nerve growth factor diffusivity in chondroitin sulfate gels, but not in control gels of hyaluronic acid. Furthermore, cultures of chick dorsal root ganglia ...
Source: Biomatter - November 20, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
Development of affinity-based delivery of NGF from a chondroitin sulfate biomaterial.
We describe the use of both chondroitin sulfate and a novel chondroitin sulfate-binding peptide to control the release of nerve growth factor. Interestingly, the novel chondroitin sulfate-binding peptide enhances the controlled release properties of the chondroitin sulfate gels. While introduction of chondroitin sulfate into a scaffold inhibits primary cortical outgrowth, the combination of chondroitin sulfate, chondroitin sulfate-binding peptide and nerve growth factor promotes primary cortical neurite outgrowth in chondroitin sulfate gels.
PMID: 23507746 [PubMed - indexed for MEDLINE] (Source: Biomatter)
Source: Biomatter - November 20, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
Factorial design-based development of measlamine microspheres for colonic delivery.
Authors: Jain V, Prasad D, Jain D, Mishra SK, Singh R
Abstract
For treating colonic diseases, conventional oral drug delivery systems are not effective, as they fail to reach the appropriate site of action. Thus, there is a need to develop effective and safe therapy for the treatment of colonic disorders. The aim of the present study was to design a colon-specific delivery system for an anti-inflammatory drug, mesalamine, with minimal degradation and optimum delivery of the drug with relatively higher local concentration, which may provide more effective therapy for inflammatory bowel disease including Cro...
Source: Biomatter - November 20, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
Designing polysaccharide-based antibacterial biomaterials for wound healing applications.
In this study, the development and characterization of novel polymer blends based on chitosan-poly (vinyl alcohol) and physically cross-linked by freeze-thaw method for possible use in a variety of biomedical application is reported. The present investigation deals with designing savlon-loaded blend hydrogels (coined as cryogels) of poly (vinyl alcohol) (PVA) and chitosan by repeated freeze-thaw method and their characterization by SEM and FTIR techniques. The FTIR spectra clearly reveal that savlon-loaded chitosan and PVA blends are bonded together through hydrogen bonding. The SEM analysis suggests that cryogels show a w...
Source: Biomatter - November 20, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
Regenerative therapy and tissue engineering for the treatment of end-stage cardiac failure: new developments and challenges.
Authors: Finosh GT, Jayabalan M
Abstract
Regeneration of myocardium through regenerative therapy and tissue engineering is appearing as a prospective treatment modality for patients with end-stage heart failure. Focusing on this area, this review highlights the new developments and challenges in the regeneration of myocardial tissue. The role of various cell sources, calcium ion and cytokine on the functional performance of regenerative therapy is discussed. The evolution of tissue engineering and the role of tissue matrix/scaffold, cell adhesion and vascularisation on tissue engineering of cardiac tissue ...
Source: Biomatter - November 20, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
Resolving the CaP-bone interface: a review of discoveries with light and electron microscopy.
Authors: Grandfield K, Palmquist A, Engqvist H, Thomsen P
Abstract
It has long been known that the interfacial relationship between synthetic materials and tissue is influential in the success of implant materials. Instability at the implant interface has been shown, in some cases, to lead to complete implant failure. Bioceramics, and in particular calcium phosphates, form a large fraction of the implantable devices on the market today due to the biocompatibility they exhibit in contact with bone and tooth-like tissues. The characterization of such bioceramic-tissue interfaces has played a crucial role in ...
Source: Biomatter - November 20, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
Maintenance of α(1)-antitrypsin activity by means of co-application of hypochlorous acid-scavengers in vitro and in the supernatant of polymorphonuclear leukocytes: as a basis for a new drug delivery approach.
Authors: Schönberg M, Reibetanz U, Rathmann S, Lessig J
Abstract
Tissue destruction, pain and loss of function in chronically inflamed tissues can result from noxious agents released from myeloperoxidase (MPO) and its highly reactive product hypochlorous acid (HOCl) or proteases such as neutrophil elastase (NE). Currently there exists a high demand for medications that provide gentle treatments, free from side effects inherent in those prescribed today. One method to circumvent side effects is through the use of locally applied drug delivery. In contrast to systemic therapy, the main advantages of transpo...
Source: Biomatter - November 20, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
Realization of a poro-elastic ultrasound replica of pulmonary tissue.
Authors: Spinelli A, Vinci B, Tirella A, Matteucci M, Gargani L, Ahluwalia A, Domenici C, Picano E, Chiarelli P
Abstract
In this work we describe the fabrication of a biocompatible hydrophilic scaffold composed of cross-linked gelatin that mimics the porous three-dimensional structure of pulmonary tissue as well as its water content and mechanical properties. The lung replica also reproduces the characteristic sonographic signs of pulmonary interstitial syndrome, the B-lines or ultrasound lung comets.
PMID: 23507784 [PubMed - indexed for MEDLINE] (Source: Biomatter)
Source: Biomatter - November 20, 2015 Category: Biotechnology Tags: Biomatter Source Type: research
