Metabolome-microbiome insights into therapeutic impact of 8-O-acetylharpagide against breast cancer in a murine model
In this study, 4 T1 breast cancer mice model was first successfully established. Then the anti-breast cancer effect of 8-O-acetylharpagide was systematically investigated. Feces were collected for metabolomics and 16S rRNA analysis to assess the potential mechanism. The results showed that 8-O-acetylharpagide was effective in reducing 4 T1 mouse tumor volume and weight compared with the model group. Metabolome analysis revealed 12 potential metabolite biomarkers in feces, mainly involved in primary bile acid biosynthesis and arachidonic acid metabolism. The 16S rRNA sequencing results demonstrated that 8-O-acetylharpagide ...
Source: Biomedical Chromatography : BMC - April 18, 2024 Category: Biomedical Science Authors: Jiahui Qian Xinyu Zhao Siyuan Yuan Sijia Su Chang Chen Junfeng Gao Xu Tang Siye Men Binyu Wen Source Type: research
A fast and efficient liquid chromatography-tandem mass spectrometry method for measuring l- and d-amino acids in the urine of patients with immunoglobulin A nephropathy
This study introduces a nonderivative LC-tandem mass spectrometry approach, offering efficient separation outcomes within 15 min for identifying chiral amino acids in human urine samples. Subsequently, using this method, the analysis of l- and d-amino acids in the urine of both patients with IgAN and healthy individuals was conducted. Fourteen d-amino acids and 20 l-amino acids were identified in the urine samples obtained from 17 patients with IgAN and 21 healthy individuals. The results indicated notable variances in the concentrations of both l- and d-amino acids between the IgAN and healthy control groups. In contrast ...
Source: Biomedical Chromatography : BMC - April 15, 2024 Category: Biomedical Science Authors: Zhijian Zha Ruihua Wang Qian Wang Fahui Chen Ziyang Ye Yafeng Li Source Type: research
Rapid identification of potential nonsteroidal anti-inflammatory drug overdose-induced liver toxicity and prediction of follow-up exposure: Integrating bioanalytical and population pharmacokinetic assay
Biomed Chromatogr. 2024 Apr 15:e5877. doi: 10.1002/bmc.5877. Online ahead of print.ABSTRACTNonsteroidal anti-inflammatory drugs (NSAIDs) are among the most frequently used drugs that can cause liver toxicity. The aim of this study was to integrate bioanalytical and population pharmacokinetic (PopPK) assay to rapidly screen and quantify the concentrations of NSAIDs in plasma and monitor clinical safety. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous quantification of acetaminophen (APAP), flurbiprofen (FLB), aspirin (ASP), and ibuprofen (IBP), four commonly used NSAIDs....
Source: Biomedical Chromatography : BMC - April 15, 2024 Category: Biomedical Science Authors: Sufeng Zhou Tangping Zhao Jie Wang Luming Wang Zimo Zhang Jinying Zhu Yuqing Zhao Ying Zhu Hao Sun Feng Shao Source Type: research
Investigation of the mechanism of nephrotoxicity of nux-vomica by PTGS2/CYP2C9-mediated arachidonic acid pathway and Jian Pi Tong Luo compound's protective effect
This study establishes a scientific foundation for the clinical use of nux-vomica and lays groundwork for further research and safety assessment of toxic Chinese herbal medicines.PMID:38618996 | DOI:10.1002/bmc.5859 (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - April 15, 2024 Category: Biomedical Science Authors: Na Zhang Baisong An Liangyou Zhao Dapeng Zhao Bochuan Lv Shumin Liu Source Type: research
A fast and efficient liquid chromatography-tandem mass spectrometry method for measuring l- and d-amino acids in the urine of patients with immunoglobulin A nephropathy
This study introduces a nonderivative LC-tandem mass spectrometry approach, offering efficient separation outcomes within 15 min for identifying chiral amino acids in human urine samples. Subsequently, using this method, the analysis of l- and d-amino acids in the urine of both patients with IgAN and healthy individuals was conducted. Fourteen d-amino acids and 20 l-amino acids were identified in the urine samples obtained from 17 patients with IgAN and 21 healthy individuals. The results indicated notable variances in the concentrations of both l- and d-amino acids between the IgAN and healthy control groups. In contrast ...
Source: Biomedical Chromatography : BMC - April 15, 2024 Category: Biomedical Science Authors: Zhijian Zha Ruihua Wang Qian Wang Fahui Chen Ziyang Ye Yafeng Li Source Type: research
Rapid identification of potential nonsteroidal anti-inflammatory drug overdose-induced liver toxicity and prediction of follow-up exposure: Integrating bioanalytical and population pharmacokinetic assay
Biomed Chromatogr. 2024 Apr 15:e5877. doi: 10.1002/bmc.5877. Online ahead of print.ABSTRACTNonsteroidal anti-inflammatory drugs (NSAIDs) are among the most frequently used drugs that can cause liver toxicity. The aim of this study was to integrate bioanalytical and population pharmacokinetic (PopPK) assay to rapidly screen and quantify the concentrations of NSAIDs in plasma and monitor clinical safety. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous quantification of acetaminophen (APAP), flurbiprofen (FLB), aspirin (ASP), and ibuprofen (IBP), four commonly used NSAIDs....
Source: Biomedical Chromatography : BMC - April 15, 2024 Category: Biomedical Science Authors: Sufeng Zhou Tangping Zhao Jie Wang Luming Wang Zimo Zhang Jinying Zhu Yuqing Zhao Ying Zhu Hao Sun Feng Shao Source Type: research
Investigation of the mechanism of nephrotoxicity of nux-vomica by PTGS2/CYP2C9-mediated arachidonic acid pathway and Jian Pi Tong Luo compound's protective effect
This study establishes a scientific foundation for the clinical use of nux-vomica and lays groundwork for further research and safety assessment of toxic Chinese herbal medicines.PMID:38618996 | DOI:10.1002/bmc.5859 (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - April 15, 2024 Category: Biomedical Science Authors: Na Zhang Baisong An Liangyou Zhao Dapeng Zhao Bochuan Lv Shumin Liu Source Type: research
Method development and validation for the estimation of gedatolisib in mouse plasma by tandem mass spectrometry and its application to pharmacokinetics studies
Biomed Chromatogr. 2024 Apr 10:e5878. doi: 10.1002/bmc.5878. Online ahead of print.ABSTRACTA simple and a sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the quantification of gedatolisib in mouse plasma. The extraction technique involved a simple precipitation method to extract gedatolisib and idelalisib (internal standard) from mouse plasma. A clean chromatographic separation of gedatolisib and the internal standard was achieved on an Atlantis dC18 column using an isocratic mobile phase (10 mm ammonium formate and acetonitrile; 30:70% v/v, both supplemented with 0.1% formic...
Source: Biomedical Chromatography : BMC - April 11, 2024 Category: Biomedical Science Authors: Vinod A B Arunava Das Source Type: research
Online preconcentration and determination of five alkaloids in Yangxinshi tablet by large-volume sample stacking and micelle to solvent stacking in cyclodextrin-modified electrokinetic chromatography
Biomed Chromatogr. 2024 Apr 10:e5876. doi: 10.1002/bmc.5876. Online ahead of print.ABSTRACTThe two-step preconcentration technique consisting of large-volume sample stacking (LVSS) and micelle to solvent stacking (MSS) in cyclodextrin-modified electrokinetic chromatography (CDEKC) was developed for the analysis of five cationic alkaloids in complex Chinese herbal prescriptions. Relevant parameters affecting separation and stacking performance were optimized separately. Under the optimal LVSS-MSS-CDEKC conditions, less analysis time and organic solvent were required, and the enhancement factors of analytes ranged from 12 to...
Source: Biomedical Chromatography : BMC - April 11, 2024 Category: Biomedical Science Authors: Rui Zhou Wenping Liu Jin Li Kunze Du Jun He Yaqi Yao Yanxu Chang Source Type: research
Method development and validation for the estimation of gedatolisib in mouse plasma by tandem mass spectrometry and its application to pharmacokinetics studies
Biomed Chromatogr. 2024 Apr 10:e5878. doi: 10.1002/bmc.5878. Online ahead of print.ABSTRACTA simple and a sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the quantification of gedatolisib in mouse plasma. The extraction technique involved a simple precipitation method to extract gedatolisib and idelalisib (internal standard) from mouse plasma. A clean chromatographic separation of gedatolisib and the internal standard was achieved on an Atlantis dC18 column using an isocratic mobile phase (10 mm ammonium formate and acetonitrile; 30:70% v/v, both supplemented with 0.1% formic...
Source: Biomedical Chromatography : BMC - April 11, 2024 Category: Biomedical Science Authors: Vinod A B Arunava Das Source Type: research
Online preconcentration and determination of five alkaloids in Yangxinshi tablet by large-volume sample stacking and micelle to solvent stacking in cyclodextrin-modified electrokinetic chromatography
Biomed Chromatogr. 2024 Apr 10:e5876. doi: 10.1002/bmc.5876. Online ahead of print.ABSTRACTThe two-step preconcentration technique consisting of large-volume sample stacking (LVSS) and micelle to solvent stacking (MSS) in cyclodextrin-modified electrokinetic chromatography (CDEKC) was developed for the analysis of five cationic alkaloids in complex Chinese herbal prescriptions. Relevant parameters affecting separation and stacking performance were optimized separately. Under the optimal LVSS-MSS-CDEKC conditions, less analysis time and organic solvent were required, and the enhancement factors of analytes ranged from 12 to...
Source: Biomedical Chromatography : BMC - April 11, 2024 Category: Biomedical Science Authors: Rui Zhou Wenping Liu Jin Li Kunze Du Jun He Yaqi Yao Yanxu Chang Source Type: research
Method development and validation for the estimation of gedatolisib in mouse plasma by tandem mass spectrometry and its application to pharmacokinetics studies
Biomed Chromatogr. 2024 Apr 10:e5878. doi: 10.1002/bmc.5878. Online ahead of print.ABSTRACTA simple and a sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the quantification of gedatolisib in mouse plasma. The extraction technique involved a simple precipitation method to extract gedatolisib and idelalisib (internal standard) from mouse plasma. A clean chromatographic separation of gedatolisib and the internal standard was achieved on an Atlantis dC18 column using an isocratic mobile phase (10 mm ammonium formate and acetonitrile; 30:70% v/v, both supplemented with 0.1% formic...
Source: Biomedical Chromatography : BMC - April 11, 2024 Category: Biomedical Science Authors: Vinod A B Arunava Das Source Type: research
Online preconcentration and determination of five alkaloids in Yangxinshi tablet by large-volume sample stacking and micelle to solvent stacking in cyclodextrin-modified electrokinetic chromatography
Biomed Chromatogr. 2024 Apr 10:e5876. doi: 10.1002/bmc.5876. Online ahead of print.ABSTRACTThe two-step preconcentration technique consisting of large-volume sample stacking (LVSS) and micelle to solvent stacking (MSS) in cyclodextrin-modified electrokinetic chromatography (CDEKC) was developed for the analysis of five cationic alkaloids in complex Chinese herbal prescriptions. Relevant parameters affecting separation and stacking performance were optimized separately. Under the optimal LVSS-MSS-CDEKC conditions, less analysis time and organic solvent were required, and the enhancement factors of analytes ranged from 12 to...
Source: Biomedical Chromatography : BMC - April 11, 2024 Category: Biomedical Science Authors: Rui Zhou Wenping Liu Jin Li Kunze Du Jun He Yaqi Yao Yanxu Chang Source Type: research
Method development and validation for the estimation of gedatolisib in mouse plasma by tandem mass spectrometry and its application to pharmacokinetics studies
Biomed Chromatogr. 2024 Apr 10:e5878. doi: 10.1002/bmc.5878. Online ahead of print.ABSTRACTA simple and a sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the quantification of gedatolisib in mouse plasma. The extraction technique involved a simple precipitation method to extract gedatolisib and idelalisib (internal standard) from mouse plasma. A clean chromatographic separation of gedatolisib and the internal standard was achieved on an Atlantis dC18 column using an isocratic mobile phase (10 mm ammonium formate and acetonitrile; 30:70% v/v, both supplemented with 0.1% formic...
Source: Biomedical Chromatography : BMC - April 11, 2024 Category: Biomedical Science Authors: Vinod A B Arunava Das Source Type: research
Online preconcentration and determination of five alkaloids in Yangxinshi tablet by large-volume sample stacking and micelle to solvent stacking in cyclodextrin-modified electrokinetic chromatography
Biomed Chromatogr. 2024 Apr 10:e5876. doi: 10.1002/bmc.5876. Online ahead of print.ABSTRACTThe two-step preconcentration technique consisting of large-volume sample stacking (LVSS) and micelle to solvent stacking (MSS) in cyclodextrin-modified electrokinetic chromatography (CDEKC) was developed for the analysis of five cationic alkaloids in complex Chinese herbal prescriptions. Relevant parameters affecting separation and stacking performance were optimized separately. Under the optimal LVSS-MSS-CDEKC conditions, less analysis time and organic solvent were required, and the enhancement factors of analytes ranged from 12 to...
Source: Biomedical Chromatography : BMC - April 11, 2024 Category: Biomedical Science Authors: Rui Zhou Wenping Liu Jin Li Kunze Du Jun He Yaqi Yao Yanxu Chang Source Type: research
