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435 records returned

Enzyme-mediated amperometric biosensors prepared via successive surface-initiated atom-transfer radical polymerization.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The development of enzyme-mediated amperometric biosensors on the indium-tin oxide (ITO) glass electrode via surface-initiated atom-transfer radical polymerization (ATRP) was investigated. A trichlorosilane coupling agent, containing the sulfonyl halide ATRP initiator, was immobilized initially on the ITO electrode surface for consecutive surface-initiated ATRP of ferrocenylmethyl methacrylate (FMMA) and glycidyl methacrylate (GMA). Glucose oxidase (GOD) was subsequently immobilized on the modified ITO electrode surface via coupling reactions between the epoxide groups of GMA and the amine groups of GOD. The surface co...
Source: Biosensors & Bioelectronics - October 1, 2009 Category: Biotechnology Authors: Zhang ZB, Yuan SJ, Zhu XL, Neoh KG, Kang ET Tags: Biosens Bioelectron Source Type: journals

Near-infrared luminescence quenching method for the detection of phenolic compounds using N-acetyl-l-cysteine-protected gold nanoparticles-tyrosinase hybrid material.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A rapid and simple near-infrared (NIR) luminescence quenching method for the detection of phenolic compounds based on combining the unique property of N-acetyl-l-cysteine-protected gold nanoparticles (NAC-AuNPs) and tyrosinase (Tyr) enzymatic reactions is described. This method relies on the luminescence quenching of NAC-AuNPs-tyrosinase (NAC-AuNPs-Tyr) hybrid material by phenolic compounds. The quinone intermediates produced from enzymatic catalytic oxidation of phenolic compounds were believed to play a major role in the luminescence quenching. Dynamic quenching mechanism was confirmed by using time-resolved luminesc...
Source: Biosensors & Bioelectronics - September 26, 2009 Category: Biotechnology Authors: Dong W, Dong C, Shuang S, Choi MM Tags: Biosens Bioelectron Source Type: journals

Sensing purine nucleoside phosphorylase activity by using silver nanoparticles.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this paper, an electrochemical biosensor to assay purine nucleoside phosphorylase (PNP) activity is reported. Due to the facilitation of sliver nanoparticles, which are modified on an electrode surface, to the electron transfer reactivity of guanosine and guanine, the electrochemical response of these species can clearly reveal the activity of the enzyme PNP. This electrochemical biosensor for the assay of PNP activity may have a broad linear range of 4-20unit/mL with a detection limit of 0.1unit/mL, which is good enough for clinical applications. Meanwhile, on the basis of the finding that guanosine and guanine can...
Source: Biosensors & Bioelectronics - September 22, 2009 Category: Biotechnology Authors: Cao Y, Wang J, Xu Y, Li G Tags: Biosens Bioelectron Source Type: journals

An enzyme-amplified amperometric DNA hybridisation assay using DNA immobilised in a carboxymethylated dextran film anchored to a graphite surface.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This report describes a simple methodology for preparation of an enzyme-amplified amperometric DNA hybridisation assay using solution-phase ferrocenemethanol mediation of glucose oxidase oxidation of glucose. The recognition layer consists of amine-terminated ssDNA (designed for binding of the sequence ssrA gene of Listeria monocytogenes) bound within a carboxymethylated dextran film that is anchored to a graphite electrode. Anchoring sites are provided by electrochemical grafting of an arylamine to the carbon surface following in situ diazotisation of p-phenylenediamine. Hybridisation between the immobilised probe ssDNA a...
Source: Biosensors & Bioelectronics - September 22, 2009 Category: Biotechnology Authors: Hajdukiewicz J, Boland S, Kavanagh P, Leech D Tags: Biosens Bioelectron Source Type: journals

Preparation and optimization of a bienzymic biosensor based on self-assembled monolayer modified gold electrode for alcohol and glucose detection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this project was to develop a bienzymic biosensor, which was based on co-immobilization of alcohol oxidase and glucose oxidase on the same electrode by formation of self-assembled monolayer (SAM) for selective determination of ethanol and glucose. In the biosensor construction the enzymes and the mediator, tetrathiafulvalene (TTF), were immobilized with cross-linking agents glutaraldehyde and cysteamine by forming a self-assembled monolayer (SAM) on a gold disc electrode. Amounts of ethanol and glucose were amperometrically detected by monitoring current values at reduction potential of TTF(+), 0.1V. Decreas...
Source: Biosensors & Bioelectronics - September 19, 2009 Category: Biotechnology Authors: Asav E, Akyilmaz E Tags: Biosens Bioelectron Source Type: journals

Ultrasensitive electrochemical sensor for mercury (II) based on target-induced structure-switching DNA.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A novel electrochemical sensor has been developed for sensitive and selective detection of mercury (II) based on target-induced structure-switching DNA. A 33-mer oligonucleotide 1 with five self-complementary base pairs separated by seven thymine-thymine mismatches was first immobilized on the electrode via self-assembly of the terminal thiol moiety and then hybridized with a ferrocene-tagged oligonucleotide 2, leading to a high redox current. In the presence of Hg(2+), mercury-mediated base pairs (T-Hg(2+)-T) induced the folding of the oligonucleotide 1 into a hairpin structure, resulting in the release of the ferroce...
Source: Biosensors & Bioelectronics - September 17, 2009 Category: Biotechnology Authors: Wu D, Zhang Q, Chu X, Wang H, Shen G, Yu R Tags: Biosens Bioelectron Source Type: journals

Label-free and time-resolved measurements of cell volume changes by surface plasmon resonance (SPR) spectroscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we demonstrate that surface plasmon resonance spectroscopy (SPR) is a powerful transducer device capable of reporting volume changes of cells that are directly grown on the SPR sensor surface. The approach is label-free, non-invasive and provides an outstanding time resolution. In proof-of-principle studies we recorded the volume change of confluent MDCK II cells induced by hypo- or hypertonic stimulation in a time-resolved manner. Comparison of the SPR-based experiments reported here with more recent studies using different approaches suggests a direct correlation between SPR signal shift and cell volume ch...
Source: Biosensors & Bioelectronics - September 16, 2009 Category: Biotechnology Authors: Robelek R, Wegener J Tags: Biosens Bioelectron Source Type: journals

An ultrasensitive signal-on electrochemical aptasensor via target-induced conjunction of split aptamer fragments.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this paper, we introduced a signal-on electrochemical sensor based on target-induced split aptamer fragments conjunction. To construct the aptasensor, the sequence of the 15-base anti-thrombin DNA aptamer was split into two fragments, one of which was attached to a gold electrode via thiol self-assembled monolayer chemistry and the second of which was modified with the redox moiety ferrocene. Thrombin-induced association of the two fragments thus increased the concentration of ferrocene at the electrode surface, which could be readily monitored via voltammetry. The sensitivity of the proposed electrochemical aptasen...
Source: Biosensors & Bioelectronics - September 16, 2009 Category: Biotechnology Authors: Chen J, Zhang J, Li J, Yang HH, Fu F, Chen G Tags: Biosens Bioelectron Source Type: journals

Detection of oligonucleotide systematic mismatches with a surface plasmon resonance sensor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The detection and parallel characterization of the hybridization event of 21-base, unlabeled DNA oligonucleotides with a monolayer of complementary DNA immobilized on a gold surface by surface plasmon resonance (SPR) is presented. A thiol modification on the probe DNA strand allowed for its attachment to the surface via self-assembly. For the hybridization of full match DNA a detection limit of 20pM was determined. The change in SPR signal was always larger for the full match compared to the one-mismatch target DNA oligonucleotides when the mismatch was in the middle or at the proximal end of the target DNA. Hybridizat...
Source: Biosensors & Bioelectronics - September 15, 2009 Category: Biotechnology Authors: Milkani E, Morais S, Lambert CR, McGimpsey WG Tags: Biosens Bioelectron Source Type: journals

Real-time, label-free monitoring of the cell cycle with a cellular impedance sensing chip.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The cell cycle plays a crucial role in many cellular physiological processes and has drawn an increasing interest in past decades. In the current study, we have developed a bioelectronic chip-based system capable of performing real-time dynamic analysis of the cell cycle in live cells via non-invasive cellular impedance sensing. The cells were cultured on the cellular impedance sensing chip comprising microfabricated interdigitated electrode structures. HeLa cells were synchronized with double thymidine block and the cellular impedances were monitored in a time resolution of minutes during the whole 5 days' experiment....
Source: Biosensors & Bioelectronics - September 15, 2009 Category: Biotechnology Authors: Wang L, Wang L, Yin H, Xing W, Yu Z, Guo M, Cheng J Tags: Biosens Bioelectron Source Type: journals

Quantum dot-based HIV capture and imaging in a microfluidic channel.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Globally, over 33.2 million people who mostly live in developing countries with limited access to the appropriate medical care suffer from the human immunodeficiency virus (HIV) infection. We developed an on-chip HIV capture and imaging method using quantum dots (Qdots) from fingerprick volume (10 microl) of unprocessed HIV-infected patient whole blood in anti-gp120 antibody-immobilized microfluidic chip. Two-color Qdots (Qdot525 and Qdot655 streptavidin conjugates) were used to identify the captured HIV by simultaneous labeling the envelope gp120 glycoprotein and its high-mannose glycans. This dual-stain imaging techn...
Source: Biosensors & Bioelectronics - August 12, 2009 Category: Biotechnology Authors: Kim YG, Moon S, Kuritzkes DR, Demirci U Tags: Biosens Bioelectron Source Type: journals

Cascade enzyme-linked immunosorbent assay (CELISA).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study represents the first example that incorporated an enzyme cascading step in an ELISA system, resulting in successful signal amplification with sensitive detection of pathogenic antigens in serum. PMID: 19665363 [PubMed - as supplied by publisher]
Source: Biosensors & Bioelectronics - July 18, 2009 Category: Biotechnology Authors: Lee YM, Jeong Y, Kang HJ, Chung SJ, Chung BH Tags: Biosens Bioelectron Source Type: journals

Singly labeled smart probes for real-time monitoring of the kinetics of dNTP misincorporation and single nucleotide extension in DNA intra-molecular polymerization.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this paper, a simple and rapid method was developed for real-time monitoring of the kinetics of dNTP misincorporation and single nucleotide extension in DNA intra-molecular polymerization by using singly labeled fluorophore-oligonucleotide smart probes. The probes are designed with a self-complementary 3'-end and a sequence of stacked cytosines at the 5'-end, to which a fluorescein (FAM) is attached. When the DNA polymerase is introduced, it will bind to the 3'-end of the probe and catalyze the extension reaction, resulting in the formation of stacked guanines, which will instantly quench the fluorescence of FAM thr...
Source: Biosensors & Bioelectronics - July 17, 2009 Category: Biotechnology Authors: Song C, Zhang C, Zhao M Tags: Biosens Bioelectron Source Type: journals

Selective recognition and removal of chlorophenols from aqueous solution using molecularly imprinted polymer prepared by reversible addition-fragmentation chain transfer polymerization.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Molecularly imprinted polymer (MIP) for selective recognition and removal of chlorophenols from contaminated water was prepared by reversible addition-fragmentation chain transfer (RAFT) polymerization using RAFT agent functionalized silica gel as the chain transfer agent. The prepared MIP nano-film hybrid silica gel (silica-MIP) was characterized using Fourier transform infrared spectrometer (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and BET adsorption isotherm analysis. The proper adsorption and selective recognition ability of the silica-MIP were studied by an equilibrium-adsorpti...
Source: Biosensors & Bioelectronics - July 17, 2009 Category: Biotechnology Authors: Li Y, Li X, Dong C, Li Y, Jin P, Qi J Tags: Biosens Bioelectron Source Type: journals

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1pg/mL to 1ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The corre...
Source: Biosensors & Bioelectronics - July 17, 2009 Category: Biotechnology Authors: Huang JC, Chang YF, Chen KH, Su LC, Lee CW, Chen CC, Chen YM, Chou C Tags: Biosens Bioelectron Source Type: journals

A DNA biosensor based on the detection of doxorubicin-conjugated Ag nanoparticle labels using solid-state voltammetry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This report describes an electrochemical biosensor for the detection of short DNA oligonucleotide of the avian flu virus H5N1 with sequence 5'-CCA AGC AAC AGA CTC AAA-3'. To fabricate this DNA biosensor, a gold (Au) electrode surface was modified with thiolated DNA probes with a sequence complementary to the target DNA. This modified Au electrode was incubated in a buffer solution containing the target DNA to form double-stranded DNA (ds-DNA) through hybridization. The ds-DNA on the electrode surface was then labeled with silver nanoparticles conjugated with a well-known DNA intercalator, doxorubicin. By performing cyclic ...
Source: Biosensors & Bioelectronics - July 17, 2009 Category: Biotechnology Authors: Ting BP, Zhang J, Gao Z, Ying JY Tags: Biosens Bioelectron Source Type: journals

Stripping voltammetric analysis of organophosphate pesticides using Ni/Al layered double hydroxides as solid-phase extraction.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study offers a new promising protocol for OPs analysis. PMID: 19647988 [PubMed - as supplied by publisher]
Source: Biosensors & Bioelectronics - July 16, 2009 Category: Biotechnology Authors: Gong J, Wang L, Song D, Zhu X, Zhang L Tags: Biosens Bioelectron Source Type: journals

A nano-Ni based ultrasensitive nonenzymatic electrochemical sensor for glucose: Enhancing sensitivity through a nanowire array strategy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Highly ordered Ni nanowire arrays (NiNWAs) were synthesized for the first time using a template-directed electropolymerization strategy with a nanopore polycarbonate (PC) membrane template, and their morphological characterization were examined by scanning electron microscopy (SEM) and transmission electron microscope (TEM). A NiNWAs based electrode shows very high electrochemical activity for electrocatalytic oxidation of glucose in alkaline medium, which has been utilized as the basis of the fabrication of a nonenzymatic biosensor for electrochemical detection of glucose. The biosensor can be applied to the quantific...
Source: Biosensors & Bioelectronics - July 7, 2009 Category: Biotechnology Authors: Lu LM, Zhang L, Qu FL, Lu HX, Zhang XB, Wu ZS, Huan SY, Wang QA, Shen GL, Yu RQ Tags: Biosens Bioelectron Source Type: journals

Optical biosensor-based characterization of anti-double-stranded DNA monoclonal antibodies as possible new standards for laboratory tests.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The serum determination of circulating anti-double-stranded (ds)DNA autoantibodies is a routine measure for the laboratory diagnosis of systemic lupus erythematosus. Since available assays differ substantially and no feasible calibrator is available, the aim of this study was to evaluate a recently introduced surface plasmon resonance (SPR) biosensor chip for binding studies between dsDNA and anti-dsDNA autoantibodies and to demonstrate its usefulness for the characterization of new monoclonal antibody (mAb) standards and standardization of assays. We characterized two human and one murine monoclonal anti-dsDNA antibod...
Source: Biosensors & Bioelectronics - July 3, 2009 Category: Biotechnology Authors: Buhl A, Page S, Heegaard NH, von Landenberg P, Luppa PB Tags: Biosens Bioelectron Source Type: journals

Monitoring the activation of neuronal nitric oxide synthase in brain tissue and cells with a potentiometric immunosensor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An all solid state potentiometric immunosensor (ASPI) has been developed to study the activation process of neuronal nitric oxide synthase (nNOS), the enzyme involved in the synthesis of nitric oxide generated under physiological conditions. At first, an all solid state H(+)-selective ISE was fabricated with the carboxylated poly(vinyl chloride) (PVC-COOH) film containing H(+) ionophore, antibody was then immobilized on the polymer layer. The immunocomplex formation was detected by monitoring pH change due to interaction between urease labeled secondary antibody and antigen. Experimental parameters such as the amount o...
Source: Biosensors & Bioelectronics - July 3, 2009 Category: Biotechnology Authors: Koh WC, Choe ES, Lee DK, Chang SC, Shim YB Tags: Biosens Bioelectron Source Type: journals

Effects of Mn(2+) on oligonucleotide-gold nanoparticle hybrids for colorimetric sensing of Hg(2+): Improving colorimetric sensitivity and accelerating color change.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this paper, we present a simple and rapid colorimetric assay - using the polythymine oligonucleotide T(33), citrate-capped gold nanoparticles (AuNPs), and phosphate-buffer saline (PBS) in the presence of Mn(2+) - for the highly selective and sensitive detection of Hg(2+) in an aqueous solution. Citrate-capped AuNPs adsorbed on randomly coiled T(33) were dispersed well in PBS because of strong electrostatic repulsion between DNA molecules. In the presence of Hg(2+), the formation of Hg(2+)-T(33) complexes enabled the removal of T(33) molecules from the NP surface, resulting in salt-induced NP aggregation. However, th...
Source: Biosensors & Bioelectronics - July 2, 2009 Category: Biotechnology Authors: Yu CJ, Cheng TL, Tseng WL Tags: Biosens Bioelectron Source Type: journals

AC-electrophoretic deposition of glucose oxidase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The electrophoretic deposition of glucose oxidase from water using asymmetrical alternating voltages is investigated. Using asymmetric voltages, glucose oxidase layers with a thickness of 7mum could be deposited on a platinum electrode in 20min time as verified with a microbalance, carbon analysis and scanning electron microscopy. In contrast, if a symmetrical alternating signal is used under the same conditions, a layer of 0.5mum is formed. We believe the deposition is due to two effects: the electrophoretic migration of the enzyme towards the deposition electrode and the pH induced precipitation of the enzyme near th...
Source: Biosensors & Bioelectronics - July 1, 2009 Category: Biotechnology Authors: Ammam M, Fransaer J Tags: Biosens Bioelectron Source Type: journals

Electrochemical detection of natural DNA damage induced by ferritin/ascobic acid/H(2)O(2) system and amplification of DNA damage by endonuclease Fpg.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Oppositely charged natural DNA and chitosan (CS) were assembled into (CS/DNA)(n) layer-by-layer films on electrode surface, and Ru(bpy)(3)(2+) (bpy=bipyridyl) in solution was used as electroactive catalyst to detect damage of DNA in the films after incubation of the films in ferritin/AA/H(2)O(2) solutions (AA=ascorbic acid). The mechanism of DNA damage caused by the ferritin/AA/H(2)O(2) system was similar to that of Fenton reaction, where the reaction of ferritin with AA would release some Fe(II) ions from ferritin and the following reaction between Fe(II) ions and H(2)O(2) would produce hydroxyl radical, which could i...
Source: Biosensors & Bioelectronics - July 1, 2009 Category: Biotechnology Authors: Liu Y, Hu N Tags: Biosens Bioelectron Source Type: journals

Electrogenerated chemiluminescence ethanol biosensor based on alcohol dehydrogenase functionalized Ru(bpy)(3)(2+) doped silica nanoparticles.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An ethanol biosensor, based on the electrogenerated chemiluminescence of Ru(bpy)(3)(2+)-doped silica nanoparticles (RuSiNPs), was investigated in this study. The biosensor was a modified glassy carbon electrode, where alcohol dehydrogenase was crosslinked to RuSiNPs, and then immobilized on the electrode surface using chitosan. The results indicated that the biosensor exhibited excellent performance during ethanol determination with a wide linear range (10(-7) to 10(-2)M), low detection limit (5.0x10(-8)M) and good stability. PMID: 19616932 [PubMed - as supplied by publisher]
Source: Biosensors & Bioelectronics - June 26, 2009 Category: Biotechnology Authors: Jia TT, Cai ZM, Chen XM, Lin ZJ, Huang XL, Chen X, Chen GN Tags: Biosens Bioelectron Source Type: journals

Quantification of DNA and protein adsorption by optical phase shift.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present a calibration method for phase shift measurements and apply it to surface-bound bovine serum albumin, immunoglobulin G, and single-stranded DNA. Biomolecules with known concentrations dissolved in salt-free water were spotted with precise volumes on the array surface and upon evaporation of the water, left a readily calculated mass. Using our label-free technique, the calculated mass of the biolayer was compared with the measured thickness, and we observed a linear dependence over 4 orders of magnitude. We determined that the widely accepted conversion of 1nm of thickness corresponds to approximately 1ng/mm(2) s...
Source: Biosensors & Bioelectronics - June 26, 2009 Category: Biotechnology Authors: Ozkumur E, Yalçın A, Cretich M, Lopez CA, Bergstein DA, Goldberg BB, Chiari M, Unlü MS Tags: Biosens Bioelectron Source Type: journals

Toward real-time continuous brain glucose and oxygen monitoring with a smart catheter.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Oxygen and glucose biosensors have been designed, fabricated, characterized and optimized for real-time continuous monitoring on a new smart catheter for use in patients with traumatic brain injury (TBI). Oxygen sensors with three-electrode configuration were designed to achieve zero net oxygen consumption. Glucose sensors were based on the use of platinum nanoparticle-enhanced electrodes that were modified with polycation and glucose oxidase immobilized by chitosan matrix. An iridium oxide electrode was developed to work as a biocompatible reference electrode with enhanced durability and stability in the biological so...
Source: Biosensors & Bioelectronics - June 26, 2009 Category: Biotechnology Authors: Li C, Ahn CH, Shutter LA, Narayan RK Tags: Biosens Bioelectron Source Type: journals

Amplified electrochemical aptasensor for thrombin based on bio-barcode method.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study, an electrochemical aptasensor for highly sensitive detection of thrombin was developed based on bio-barcode amplification assay. For this proposed aptasensor, capture DNA aptamerI was immobilized on the Au electrode. The functional Au nanoparticles (DNA-AuNPs) are loaded with barcode binding DNA and aptamerII. Through the specific recognition for thrombin, a sandwich format of Au/aptamerI/thrombin/DNA-AuNPs was fabricated. After hybridization with the PbSNPs-labeled barcode DNA, the assembled sensor was obtained. The concentration of thrombin was monitored based on the concentration of lead ions d...
Source: Biosensors & Bioelectronics - June 24, 2009 Category: Biotechnology Authors: Zhang X, Qi B, Li Y, Zhang S Tags: Biosens Bioelectron Source Type: journals

Cold plasma functionalized TeraHertz BioMEMS for enzyme reaction analysis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this paper, we describe the development, functionalization and functionality testing of a TeraHertz (THz) Bio-MicroElectroMechanical System (BioMEMS) dedicated to enzyme reaction analysis. The microdevice was fabricated by mixing clean room microfabrication with cold plasma deposition. The first is used to build the microfluidic circuits and the THz sensor, while the later serves for the polymerization of allylamine using a homemade glow discharge plasma reactor for a subsequent immobilization of enzymatic biocatalysts. Thermal stability of the deposited plasma polymer has been investigated by infrared spectroscopy....
Source: Biosensors & Bioelectronics - June 24, 2009 Category: Biotechnology Authors: Abbas A, Treizebre A, Supiot P, Bourzgui NE, Guillochon D, Vercaigne-Marko D, Bocquet B Tags: Biosens Bioelectron Source Type: journals

Intervention of cardiomyocyte death based on real-time monitoring of cell adhesion through impedance sensing.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cardiomyocyte death caused by proinflammatory cytokines, such as Tumor necrosis factor alpha (TNF-alpha), is one of the hot topics in cardiovascular research. TNF-alpha can induce multiple cell processes that are dependent on the treatment time although the long-term treatment definitely leads to cell death. The ability to intervene in cell death will be invaluable to reveal the effects of short-term TNF-alpha treatment to cardiomyocytes. However, a real-time monitoring technique is needed to guide the intervention of cell responses. In this work, we employed the impedance-sensing technique to real-time monitor the equ...
Source: Biosensors & Bioelectronics - June 24, 2009 Category: Biotechnology Authors: Qiu Y, Liao R, Zhang X Tags: Biosens Bioelectron Source Type: journals

Novel application of surface plasmon resonance biosensor chips for measurement of advanced glycation end products in serum of Zucker diabetic fatty rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Advanced glycation end products (AGEs) have been implicated in diabetic complications. To measure AGEs, especially N(varepsilon)-(carboxymethyl)lysine (CML), in sera from Zucker diabetic fatty rats (ZDF) and Zucker lean rats (ZL), we used a novel method of protein chip and surface plasmon resonance imaging (SPRI). Serum samples were obtained from male ZDF and ZL rats at 20 weeks of age. Antibodies to AGEs or CML were immobilized on a gold surface, which was modified by cysteine-tagged, protein-G constructs. The gold chip upon which the serum was spotted was optically coupled with a prism coupler. The reflected images f...
Source: Biosensors & Bioelectronics - June 18, 2009 Category: Biotechnology Authors: Kim YS, Yi SY, Kim J, Kim M, Kim CS, Chung BH, Kim JS Tags: Biosens Bioelectron Source Type: journals

A disposable and cost efficient microfluidic device for the rapid chip-based electrical detection of DNA.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Requirements for a point-of-care device are an easy and robust read-out and - above all - a simple handling. We integrated an established robust electrical read-out for DNA-chips into a microfluidic device, thereby creating an automated analysis system that combines the necessary steps for a chip-based analysis. It is based on the electrical detection of biotin-labeled DNA in a gap between two microstructured electrodes on the surface of a DNA-chip. The biotin serves as binding molecule for streptavidin-conjugated horseradish peroxidase. A following enzyme-induced silver deposition bridges the gap by a conductive layer...
Source: Biosensors & Bioelectronics - June 18, 2009 Category: Biotechnology Authors: Schüler T, Kretschmer R, Jessing S, Urban M, Fritzsche W, Möller R, Popp J Tags: Biosens Bioelectron Source Type: journals

Design and application of a flexible and implantable sensor for detecting uterine musculature contraction.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It is very important to obtain the parameters of deformation size and contraction frequency of women's uterine musculature in medical research. This paper proposes a type of sensor for measuring these parameters and analyzes its force situation. The flexibility of the sensor makes it easy for doctors to let the sensor pass through woman's narrow cervix and get to her uterine cavity. The experiment shows that the sensitivity of three coils in the sample sensor achieves 22.38nH/mm(2) both for left and right coils, 22.84nH/mm(2) for top coil, which can meet the requirements of sensitivity for testing the contraction situa...
Source: Biosensors & Bioelectronics - June 18, 2009 Category: Biotechnology Authors: Zhao G, Qin L, Yang Q, Wu S, He P Tags: Biosens Bioelectron Source Type: journals

Graphitized macroporous carbon microarray with hierarchical mesopores as host for the fabrication of electrochemical biosensor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A novel graphitized ordered macroporous carbon (GMC, pore size approximately 380nm) with hierarchical mesopores (2-30nm) and high graphitization degree was prepared by nickel-catalyzed graphitization of polystyrene arrays. The obtained GMC possessed high specific surface area, large pore volume, and good electrical conductivity, which was explored for the enzyme entrapment and biosensor fabrication by a facile method. With advantages of novel nanostructure and good electrical conductivity, direct electrochemistry of hemoglobin (a model protein) was observed on the GMC-based biocomposite with a formal potential of -0.36...
Source: Biosensors & Bioelectronics - June 17, 2009 Category: Biotechnology Authors: Lu X, Xiao Y, Lei Z, Chen J Tags: Biosens Bioelectron Source Type: journals

Rapid and sensitive detection of microcystin by immunosensor based on nuclear magnetic resonance.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A stable and sensitive toxin residues immunosensor based on the relaxation of magnetic nanoparticles was developed. The method was performed in one reaction and offered sensitive, fast detection of target toxin residues in water. The target analyte, microcystin-LR (MC-LR) in Tai lake water, competed with the antigens on the surface of the magnetic nanoparticles and then influenced the formation of aggregates of the magnetic nanoparticles. Accordingly, the magnetic relaxation time of the magnetic nanoparticles was changed under the effect of the target analyte. The calibration curve was deduced at different concentratio...
Source: Biosensors & Bioelectronics - June 17, 2009 Category: Biotechnology Authors: Ma W, Chen W, Qiao R, Liu C, Yang C, Li Z, Xu D, Peng C, Jin Z, Xu C, Zhu S, Wang L Tags: Biosens Bioelectron Source Type: journals

A microfluidic biosensor based on competitive protein adsorption for thyroglobulin detection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a microfluidic sensing platform for the detection of thyroglobulin (Tg) using competitive protein adsorption. Serum Tg is a highly specific biomarker for residual thyroid tissue, recurrence and metastases after treatment for differentiated thyroid cancer (DTC). Conventional Tg detection techniques require complicated immobilization of antibodies and need to form a sandwich assay using additional secondary antibodies to enhance the sensitivity. We present a fundamentally different sensing technique without using antibody immobilization on a microfluidic platform. We engineer two surfaces covered by two known prote...
Source: Biosensors & Bioelectronics - June 17, 2009 Category: Biotechnology Authors: Choi S, Chae J Tags: Biosens Bioelectron Source Type: journals

Homogeneous noncompetitive assay of protein via Förster-resonance-energy-transfer with tryptophan residue(s) as intrinsic donor(s) and fluorescent ligand as acceptor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Homogeneous noncompetitive assay of protein via Förster-resonance-energy-transfer with tryptophan residue(s) as intrinsic donor(s) and fluorescent ligand as acceptor. Biosens Bioelectron. 2009 Jun 17; Authors: Liao F, Xie Y, Yang X, Deng P, Chen Y, Xie G, Zhu S, Liu B, Yuan H, Liao J, Zhao Y, Yu M Homogeneous noncompetitive assay of a protein in biological samples based on Förster-resonance-energy-transfer (FRET) was proposed by using its tryptophan residues as intrinsic donors and its specific fluorescent ligand as the FRET acceptor that was defined as an analytical FRET probe. Conjugate of a suitabl...
Source: Biosensors & Bioelectronics - June 17, 2009 Category: Biotechnology Authors: Liao F, Xie Y, Yang X, Deng P, Chen Y, Xie G, Zhu S, Liu B, Yuan H, Liao J, Zhao Y, Yu M Tags: Biosens Bioelectron Source Type: journals

"Biosens Bioelectron"[ta]; +113 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
113 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biosens Bioelectron"[ta] These pubmed results were generated on 2009/06/12PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources.
Source: Biosensors & Bioelectronics - June 13, 2009 Category: Biotechnology Tags: Report Source Type: journals

Development of redox-labeled electrochemical immunoassay for polycyclic aromatic hydrocarbons with controlled surface modification and catalytic voltammetric detection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A redox-labeled direct competitive electrochemical immunoassay for polycyclic aromatic hydrocarbons (PAHs) was developed. A ruthenium tris(bipyridine)-pyrenebutyric acid conjugate was synthesized as the redox-labeled tracer. Its recognition by an anti-PAH monoclonal antibody was confirmed by surface plasmon resonance. In the immunoassay, the antibody was immobilized on (3-glycidoxypropyl)-trimethoxysilane (GPTMS)-modified indium tin oxide (ITO) electrodes. The assay was quantified by measuring the electro-catalytic current of the redox label in an oxalate-containing electrolyte which served as a sacrificial electron do...
Source: Biosensors & Bioelectronics - March 9, 2009 Category: Biotechnology Authors: Wei MY, Wen SD, Yang XQ, Guo LH Tags: Biosens Bioelectron Source Type: journals

Improved method for impedance measurements of mammalian cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study describes the use of impedance spectroscopy as a non-invasive tool to monitor cell proliferation and morphology and to obtain better measurements of cell kinetics. The goal was to determine test frequencies at which the cell behaviour towards different chemicals and toxins can be monitored with good time resolution. For optimisation of the test frequency it is in the first instance necessary to measure an impedance spectrum of the confluent cell layer. Secondly, the frequency of the highest peak in the spectrum will be determined and further experiments will be carried out at this fixed test frequency. In contra...
Source: Biosensors & Bioelectronics - March 9, 2009 Category: Biotechnology Authors: Rümenapp C, Remm M, Wolf B, Gleich B Tags: Biosens Bioelectron Source Type: journals

Electrodeposition of chitosan-ionic liquid-glucose oxidase biocomposite onto nano-gold electrode for amperometric glucose sensing.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A sensitive glucose biosensor was fabricated by electrodepositing chitosan-ionic liquid-glucose oxidase biocomposite onto nano-gold electrode. First, nano-gold electrode was constructed by electrochemically depositing gold nanoparticles onto a flat gold electrode surface. Then the nano-gold electrode was immersed in the bath containing p-benzoquinone (BQ), chitosan (CS), glucose oxidase (GOD) and ionic liquid (IL) for electrodeposition of enzymatic electrode. The proton consumption during electroreduction of BQ increased the local solution pH near the electrode surface and led to the deposition of CS hydrogel on the el...
Source: Biosensors & Bioelectronics - March 6, 2009 Category: Biotechnology Authors: Zeng X, Li X, Xing L, Liu X, Luo S, Wei W, Kong B, Li Y Tags: Biosens Bioelectron Source Type: journals

Selective capture of a specific cell type from mixed leucocytes in an electrode-integrated microfluidic device.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a microfluidic method to selectively capture a type of leucocytes (neutrophils or eosinophils) from a suspension of mixed leucocytes in a specific region of a microchannel whose upper wall is equipped with an array of microband electrodes. One of the microelectrodes is used for locally electrogenerating hypobromous acid (HBrO) that renders the opposite face of the channel protein-adsorptive. Since the patterned layer of poly(ethylene glycol)dimethacrylate (PEGDM), impervious to HBrO, is previously introduced at the channel bottom, the region of protein adsorption can be precisely defined even in the presence of...
Source: Biosensors & Bioelectronics - March 6, 2009 Category: Biotechnology Authors: Hashimoto M, Kaji H, Nishizawa M Tags: Biosens Bioelectron Source Type: journals

Highly ordered mesoporous carbons-based glucose/O(2) biofuel cell.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study demonstrates a novel compartment-less glucose/O(2) biofuel cell (BFC) based on highly ordered mesoporous carbons (OMCs) with three-dimensionally (3D) interconnected and ordered pore structures. OMCs are used as supports for both stably confining the electrocatalyst (i.e., meldola's blue, MDB) for NADH oxidation and the anodic biocatalyst (i.e., NAD(+)-dependent glucose dehydrogenase, GDH) for glucose oxidation, and for facilitating direct electrochemistry of the cathodic biocatalyst (i.e., laccase, LAC) for O(2) electroreduction. In 0.10M pH 6.0 PBS containing 20mM NAD(+) and 60mM glucose under the air-saturated...
Source: Biosensors & Bioelectronics - March 6, 2009 Category: Biotechnology Authors: Zhou M, Deng L, Wen D, Shang L, Jin L, Dong S Tags: Biosens Bioelectron Source Type: journals

Enzyme-enhanced fluorescence detection of DNA on etched optical fibers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A novel DNA biosensor based on enzyme-enhanced fluorescence detection on etched optical fibers was developed. The hybridization complex of DNA probe and biotinylated target was formed on the etched optical fiber, and was then bound with streptavidin labeled horseradish peroxidase (streptavidin-HRP). The target DNA was quantified through the fluorescent detection of bi-p,p'-4-hydroxyphenylacetic acid (DBDA) generated from the substrate 4-hydroxyphenylacetic acid (p-HPA) under the catalysis of HRP, with a detection limit of 1pM and a linear range from 1.69pM to 169pM. It is facile to regenerate this sensor through surfac...
Source: Biosensors & Bioelectronics - March 3, 2009 Category: Biotechnology Authors: Niu SY, Li QY, Ren R, Zhang SS Tags: Biosens Bioelectron Source Type: journals

Production of surface plasmon resonance based assay kit for hepatitis diagnosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hepatitis B surface antibody (HBsAb) imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-L-tyrosine methyl ester) (PHEMAT) film on the surface plasmon resonance (SPR) sensor chip was prepared for diagnosis of HBsAb in human serum. Gold SPR chip surface was modified with allyl mercaptane and, then, HBsAb-imprinted PHEMAT film was formed on the chip surface. Surface characterization of the non-modified, allyl mercaptane modified and HBsAb-imprinted PHEMAT SPR chips were investigated with contact angle, atomic force microscopy (AFM). Kinetic studies were performed using HBsAb positive human serum. In order to determin...
Source: Biosensors & Bioelectronics - March 3, 2009 Category: Biotechnology Authors: Uzun L, Say R, Unal S, Denizli A Tags: Biosens Bioelectron Source Type: journals

Structural properties and sensing performance of high-k Nd(2)TiO(5) thin layer-based electrolyte-insulator-semiconductor for pH detection and urea biosensing.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
For high sensitive pH sensing, an electrolyte-insulator-semiconductor (EIS) device with Nd(2)TiO(5) thin layers fabricated on Si substrates by means of reactive sputtering and the subsequent post-deposition annealing (PDA) treatment was proposed. In this work, the effect of thermal annealing (600, 700, 800, and 900 degrees C) on the structural characteristics of Nd(2)TiO(5) thin layer was investigated by X-ray diffraction, X-ray photoelectron spectroscopy, and atomic force microscopy. The observed structural properties were then correlated with the resulting pH sensing performances. For enzymatic field-effect-transisto...
Source: Biosensors & Bioelectronics - February 27, 2009 Category: Biotechnology Authors: Pan TM, Lin JC, Wu MH, Lai CS Tags: Biosens Bioelectron Source Type: journals

Nanohole arrays of mixed designs and microwriting for simultaneous and multiple protein binding studies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We demonstrate using nanohole arrays of mixed designs and a microwriting process based on dip-pen nanolithography to monitor multiple, different protein binding events simultaneously in real-time based on the intensity of Extraordinary Optical Transmission of nanohole arrays. The microwriting process and small footprint of the individual nanohole arrays enabled us to observe different binding events located only 16mum apart, achieving high spatial resolution. We also present a novel concept that incorporates nanohole arrays of different designs to improve confidence and accuracy of binding studies. For proof of concept...
Source: Biosensors & Bioelectronics - February 27, 2009 Category: Biotechnology Authors: Ji J, Yang JC, Larson DN Tags: Biosens Bioelectron Source Type: journals

Automated and ultrasensitive detection of methyl-3-quinoxaline-2-carboxylic acid by using gold nanoparticles probes SIA-rt-PCR.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An ultrasensitive and rapid sequential injection analysis (SIA) based on real-time PCR (SIA-rt-PCR) assay was developed by using different nanoparticles for the detection of small molecule chemicals residues. Gold (Au) nanoparticle, conjugated with goat anti-rabbit IgG and duplex strand DNA (dsDNA), was used as a substitute for chemiluminescent probes in an SIA system. By indirect competitive immunoreactions in the SIA system, the gold nanoparticles were attached to antigens which were immobilized by superparamagnetic nanoparticles (SMNPs). The dsDNA on the gold nanoparticles was dehybridized and then the single-strand...
Source: Biosensors & Bioelectronics - February 27, 2009 Category: Biotechnology Authors: Chen W, Jiang Y, Ji B, Zhu C, Liu L, Peng C, Jin MK, Qiao R, Jin Z, Wang L, Zhu S, Xu C Tags: Biosens Bioelectron Source Type: journals

Fast picomolar selective detection of bisphenol A in water using a carbon nanotube field effect transistor functionalized with estrogen receptor-alpha.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this paper we report a biosensor for the fast, ultrasensitive and selective determination of bisphenol A in water. It is based on a field effect transistor (FET) in which a network of single-walled carbon nanotubes (SWCNTs) acts as the conductor channel. SWCNTs are functionalized for the first time with a nuclear receptor, the estrogen receptor alpha (ER-alpha), which is adsorbed onto the SWCNTs and acts as the sensing part of the biosensor. SWCTNs are subsequently protected to prevent the non-specific binding of interferences. With this biosensor we can detect picomolar concentrations of BPA in only 2min of analysi...
Source: Biosensors & Bioelectronics - February 27, 2009 Category: Biotechnology Authors: Sánchez-Acevedo ZC, Riu J, Rius FX Tags: Biosens Bioelectron Source Type: journals

A systematic investigation into the electrical properties of single HeLa cells via impedance measurements and COMSOL simulations.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The electrical properties of single cells provide fundamental insights into their pathological condition and are therefore of immense interest to medical practitioners. Accordingly, this study captures single HeLa cells using a microfluidic device and then measures their impedance properties using a commercial impedance spectroscopy system. The experimental system is modeled by an equivalent electrical circuit and COMSOL simulations are then performed to establish the conductivity, permittivity and impedance of single HeLa cells under various operational frequencies and voltages. At an operational voltage of 0.2V, the ...
Source: Biosensors & Bioelectronics - February 21, 2009 Category: Biotechnology Authors: Wang MH, Jang LS Tags: Biosens Bioelectron Source Type: journals

Electrochemiluminescence from hydrophilic thin film Ru(bpy)(3)(2+)-modified electrode prepared using natural halloysite nanotubes and polyacrylamide gel.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Because Ru(bpy)(3)(2+)-modified electrodes are often used in aqueous condition, the development of a hydrophilic modified electrode is of critical importance. Herein a hydrophilic, thin film Ru(bpy)(3)(2+)-modified electrode is successfully developed using polyacrylamide gel to embed halloysite nanotubes on the electrode surface which is used to adsorb Ru(bpy)(3)(2+) by cation-exchange. X-ray photoelectron spectroscopy demonstrates the formation of the film on an electrode and the high adsorbing capacity of the halloysite nanotubes toward Ru(bpy)(3)(2+). The different electrochemiluminescence (ECL) behaviors of the ele...
Source: Biosensors & Bioelectronics - February 21, 2009 Category: Biotechnology Authors: Xing B, Yin XB Tags: Biosens Bioelectron Source Type: journals

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