Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli
Biotechnol Adv. 2024 Apr 7;73:108353. doi: 10.1016/j.biotechadv.2024.108353. Online ahead of print.ABSTRACTL-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with Escherichia coli being extensively studied as the preferred strain. This preference is due to its ability to grow rapidly in cost-effective media, its robustness for industrial processes, the well-charact...
Source: Biotechnology Advances - April 9, 2024 Category: Biotechnology Authors: Lijuan Wang Yingying Guo Yizhou Shen Kun Yang Xue Cai Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli
Biotechnol Adv. 2024 Apr 7:108353. doi: 10.1016/j.biotechadv.2024.108353. Online ahead of print.ABSTRACTL-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with Escherichia coli being extensively studied as the preferred strain. This preference is due to its ability to grow rapidly in cost-effective media, its robustness for industrial processes, the well-characteri...
Source: Biotechnology Advances - April 9, 2024 Category: Biotechnology Authors: Lijuan Wang Yingying Guo Yizhou Shen Kun Yang Xue Cai Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli
Biotechnol Adv. 2024 Apr 7:108353. doi: 10.1016/j.biotechadv.2024.108353. Online ahead of print.ABSTRACTL-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with Escherichia coli being extensively studied as the preferred strain. This preference is due to its ability to grow rapidly in cost-effective media, its robustness for industrial processes, the well-characteri...
Source: Biotechnology Advances - April 9, 2024 Category: Biotechnology Authors: Lijuan Wang Yingying Guo Yizhou Shen Kun Yang Xue Cai Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli
Biotechnol Adv. 2024 Apr 7:108353. doi: 10.1016/j.biotechadv.2024.108353. Online ahead of print.ABSTRACTL-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with Escherichia coli being extensively studied as the preferred strain. This preference is due to its ability to grow rapidly in cost-effective media, its robustness for industrial processes, the well-characteri...
Source: Biotechnology Advances - April 9, 2024 Category: Biotechnology Authors: Lijuan Wang Yingying Guo Yizhou Shen Kun Yang Xue Cai Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids
Biotechnol Adv. 2024 Apr 6:108354. doi: 10.1016/j.biotechadv.2024.108354. Online ahead of print.ABSTRACTThraustochytrids are marine microorganisms known for their fast growth and ability to store lipids, making them useful for producing polyunsaturated fatty acids (PUFAs), biodiesel, squalene, and carotenoids. However, the high cost of production, mainly due to expensive fermentation components, limits their wider use. A significant challenge in this context is the need to balance production costs with the value of the end products. This review focuses on integrating the efficient utilization of waste with Thraustochytrids...
Source: Biotechnology Advances - April 8, 2024 Category: Biotechnology Authors: Zi-Xu Zhang Ying-Shuang Xu Zi-Jia Li Lu-Wei Xu Wang Ma Ying-Feng Li Dong-Sheng Guo Xiao-Man Sun He Huang Source Type: research
Recent advances in design and application of synthetic membraneless organelles
Biotechnol Adv. 2024 Apr 6:108355. doi: 10.1016/j.biotechadv.2024.108355. Online ahead of print.ABSTRACTMembraneless organelles (MLOs) formed by liquid-liquid phase separation (LLPS) have been extensively studied due to their spatiotemporal control of biochemical and cellular processes in living cells. These findings have provided valuable insights into the physicochemical principles underlying the formation and functionalization of biomolecular condensates, which paves the way for the development of versatile phase-separating systems capable of addressing a variety of application scenarios. Here, we highlight the potentia...
Source: Biotechnology Advances - April 8, 2024 Category: Biotechnology Authors: Li Wan Yingying Zhu Wenli Zhang Wanmeng Mu Source Type: research
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids
Biotechnol Adv. 2024 Apr 6:108354. doi: 10.1016/j.biotechadv.2024.108354. Online ahead of print.ABSTRACTThraustochytrids are marine microorganisms known for their fast growth and ability to store lipids, making them useful for producing polyunsaturated fatty acids (PUFAs), biodiesel, squalene, and carotenoids. However, the high cost of production, mainly due to expensive fermentation components, limits their wider use. A significant challenge in this context is the need to balance production costs with the value of the end products. This review focuses on integrating the efficient utilization of waste with Thraustochytrids...
Source: Biotechnology Advances - April 8, 2024 Category: Biotechnology Authors: Zi-Xu Zhang Ying-Shuang Xu Zi-Jia Li Lu-Wei Xu Wang Ma Ying-Feng Li Dong-Sheng Guo Xiao-Man Sun He Huang Source Type: research
Recent advances in design and application of synthetic membraneless organelles
Biotechnol Adv. 2024 Apr 6:108355. doi: 10.1016/j.biotechadv.2024.108355. Online ahead of print.ABSTRACTMembraneless organelles (MLOs) formed by liquid-liquid phase separation (LLPS) have been extensively studied due to their spatiotemporal control of biochemical and cellular processes in living cells. These findings have provided valuable insights into the physicochemical principles underlying the formation and functionalization of biomolecular condensates, which paves the way for the development of versatile phase-separating systems capable of addressing a variety of application scenarios. Here, we highlight the potentia...
Source: Biotechnology Advances - April 8, 2024 Category: Biotechnology Authors: Li Wan Yingying Zhu Wenli Zhang Wanmeng Mu Source Type: research
Systematic review on carrageenolytic enzymes: From metabolic pathways to applications in biotechnology
Biotechnol Adv. 2024 Apr 4:108351. doi: 10.1016/j.biotechadv.2024.108351. Online ahead of print.ABSTRACTCarrageenan, the major carbohydrate component of some red algae, is an important renewable bioresource with very large annual outputs. Different types of carrageenolytic enzymes in the carrageenan metabolic pathway are potentially valuable for the production of carrageenan oligosaccharides, biofuel, and other chemicals obtained from carrageenan. However, these enzymes are not well-developed for oligosaccharide or biofuel production. For further application, comprehensive knowledge of carrageenolytic enzymes is essential....
Source: Biotechnology Advances - April 6, 2024 Category: Biotechnology Authors: Chengcheng Jiang Yuqi Ma Wei Wang Jingjing Sun Jianhua Hao Xiangzhao Mao Source Type: research
Systematic review on carrageenolytic enzymes: From metabolic pathways to applications in biotechnology
Biotechnol Adv. 2024 Apr 4:108351. doi: 10.1016/j.biotechadv.2024.108351. Online ahead of print.ABSTRACTCarrageenan, the major carbohydrate component of some red algae, is an important renewable bioresource with very large annual outputs. Different types of carrageenolytic enzymes in the carrageenan metabolic pathway are potentially valuable for the production of carrageenan oligosaccharides, biofuel, and other chemicals obtained from carrageenan. However, these enzymes are not well-developed for oligosaccharide or biofuel production. For further application, comprehensive knowledge of carrageenolytic enzymes is essential....
Source: Biotechnology Advances - April 6, 2024 Category: Biotechnology Authors: Chengcheng Jiang Yuqi Ma Wei Wang Jingjing Sun Jianhua Hao Xiangzhao Mao Source Type: research
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications
Biotechnol Adv. 2024 Apr 3;72:108352. doi: 10.1016/j.biotechadv.2024.108352. Online ahead of print.ABSTRACTNitrilases represent a distinct class of enzymes that play a pivotal role in catalyzing the hydrolysis of nitrile compounds, leading to the formation of corresponding carboxylic acids. These enzymatic entities have garnered significant attention across a spectrum of industries, encompassing pharmaceuticals, agrochemicals, and fine chemicals. Moreover, their significance has been accentuated by mounting environmental pressures, propelling them into the forefront of biodegradation and bioremediation endeavors. Neverthel...
Source: Biotechnology Advances - April 4, 2024 Category: Biotechnology Authors: Shi-Peng Zhou Ya-Ping Xue Yu-Guo Zheng Source Type: research
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications
Biotechnol Adv. 2024 Apr 2:108352. doi: 10.1016/j.biotechadv.2024.108352. Online ahead of print.ABSTRACTNitrilases represent a distinct class of enzymes that play a pivotal role in catalyzing the hydrolysis of nitrile compounds, leading to the formation of corresponding carboxylic acids. These enzymatic entities have garnered significant attention across a spectrum of industries, encompassing pharmaceuticals, agrochemicals, and fine chemicals. Moreover, their significance has been accentuated by mounting environmental pressures, propelling them into the forefront of biodegradation and bioremediation endeavors. Nevertheless...
Source: Biotechnology Advances - April 4, 2024 Category: Biotechnology Authors: Shi-Peng Zhou Ya-Ping Xue Yu-Guo Zheng Source Type: research
Inteins: A Swiss army knife for synthetic biology
Biotechnol Adv. 2024 Mar 27:108349. doi: 10.1016/j.biotechadv.2024.108349. Online ahead of print.ABSTRACTInteins are proteins found in nature that execute protein splicing. Among them, split inteins stand out for their versatility and adaptability, presenting creative solutions for addressing intricate challenges in various biological applications. Their exquisite attributes, including compactness, reliability, orthogonality, low toxicity, and irreversibility, make them of interest to various fields including synthetic biology, biotechnology and biomedicine. In this review, we delve into the inherent challenges of using in...
Source: Biotechnology Advances - March 29, 2024 Category: Biotechnology Authors: Stanislav Anastassov Maurice Filo Mustafa Khammash Source Type: research
Inteins: A Swiss army knife for synthetic biology
Biotechnol Adv. 2024 Mar 27:108349. doi: 10.1016/j.biotechadv.2024.108349. Online ahead of print.ABSTRACTInteins are proteins found in nature that execute protein splicing. Among them, split inteins stand out for their versatility and adaptability, presenting creative solutions for addressing intricate challenges in various biological applications. Their exquisite attributes, including compactness, reliability, orthogonality, low toxicity, and irreversibility, make them of interest to various fields including synthetic biology, biotechnology and biomedicine. In this review, we delve into the inherent challenges of using in...
Source: Biotechnology Advances - March 29, 2024 Category: Biotechnology Authors: Stanislav Anastassov Maurice Filo Mustafa Khammash Source Type: research
Inteins: A Swiss army knife for synthetic biology
Biotechnol Adv. 2024 Mar 27:108349. doi: 10.1016/j.biotechadv.2024.108349. Online ahead of print.ABSTRACTInteins are proteins found in nature that execute protein splicing. Among them, split inteins stand out for their versatility and adaptability, presenting creative solutions for addressing intricate challenges in various biological applications. Their exquisite attributes, including compactness, reliability, orthogonality, low toxicity, and irreversibility, make them of interest to various fields including synthetic biology, biotechnology and biomedicine. In this review, we delve into the inherent challenges of using in...
Source: Biotechnology Advances - March 29, 2024 Category: Biotechnology Authors: Stanislav Anastassov Maurice Filo Mustafa Khammash Source Type: research
