A new look at the fluorescent protein-based approach for identifying optimal coding sequence for recombinant protein expression in E. coli
Biotechnol J. 2024 Apr;19(4):e2300343. doi: 10.1002/biot.202300343.ABSTRACTDue to the degeneracy of the genetic code, most amino acids are encoded by several codons. The choice among synonymous codons at the N-terminus of genes has a profound effect on protein expression in Escherichia coli. This is often explained by the different contributions of synonymous codons to mRNA secondary structure formation. Strong secondary structures at the 5'-end of mRNA interfere with ribosome binding and affect the process of translation initiation. In silico optimization of the gene 5'-end can significantly increase the level of protein ...
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Artur I Zabolotskii Natalia S Riabkova Source Type: research
Rainbow screening: Chromoproteins enable visualized molecular cloning
Biotechnol J. 2024 Apr;19(4):e2400114. doi: 10.1002/biot.202400114.ABSTRACTMolecular cloning facilitates the assembly of heterologous DNA fragments with vectors, resulting in the generation of plasmids that can steadily replicate in host cells. To efficiently and accurately screen out the expected plasmid candidates, various methods, such as blue-white screening, have been developed for visualization. However, these methods typically require additional genetic manipulations and costs. To simplify the process of visualized molecular cloning, here we report Rainbow Screening, a method that combines Gibson Assembly with chrom...
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Fang Ba Yufei Zhang Wan-Qiu Liu Jian Li Source Type: research
Fluid shear force and hydrostatic pressure jointly promote osteogenic differentiation of BMSCs by activating YAP1 and NFAT2
This study investigated the impact of FSS and HP on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and examined the distinctions and interactions between the two mechanisms. The results demonstrated that both FSS and HP individually enhanced the osteogenic differentiation of BMSCs, with a more pronounced effect observed through their combined application. BMSCs responded to external FSS and HP stimulation through the integrin-cytoskeleton and Piezo1 ion channel respectively. This led to the activation of downstream biochemical signals, resulting in the dephosphorylation and nuclear translocati...
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Yi Zhou Pan Guo Ziyang Jin Miaomiao Chai Shuhong Zhang Xianwei Wang Wen-Song Tan Yan Zhou Source Type: research
Recent advances in the synthesis of single-stranded DNA in vitro
Biotechnol J. 2024 Apr;19(4):e2400026. doi: 10.1002/biot.202400026.ABSTRACTSingle-stranded DNA (ssDNA) is the foundation of modern biology, with wide applications in gene editing, sequencing, DNA information storage, and materials science. However, synthesizing ssDNA with high efficiency, high throughput, and low error rate in vitro remains a major challenge. Various methods have been developed for ssDNA synthesis, and some significant results have been achieved. In this review, six main methods were introduced, including solid-phase oligonucleotide synthesis, terminal deoxynucleotidyl transferase-based ssDNA synthesis, re...
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Shuai Li Wei Tan Xuemei Jia Qing Miao Ying Liu Dayong Yang Source Type: research
Depolymerization of the polyester-polyurethane by amidase GatA250 and enhancing the production of 4,4'-methylenedianiline with cutinase LCC
This study provides valuable insights into PU pollution control and also proposes applicable solutions to manage PU wastes through bio-recycling.PMID:38622797 | DOI:10.1002/biot.202300723 (Source: Biotechnology Journal)
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Kaiyuan Xin Jianqi Lu Qingyi Zeng Tianyang Zhang Jiawei Liu Jie Zhou Weiliang Dong Min Jiang Source Type: research
Visual screening of CRISPR/Cas9 editing efficiency based on micropattern arrays for editing porcine cells
Biotechnol J. 2024 Apr;19(4):e2300691. doi: 10.1002/biot.202300691.ABSTRACTCRISPR/Cas9 technology, combined with somatic cell nuclear transplantation (SCNT), represents the primary approach to generating gene-edited pigs. The inefficiency in acquiring gene-edited nuclear donors is attributed to low editing and delivery efficiency, both closely linked to the selection of CRISPR/Cas9 forms. However, there is currently no direct method to evaluate the efficiency of CRISPR/Cas9 editing in porcine genomes. A platform based on fluorescence reporting signals and micropattern arrays was developed in this study, to visually assess ...
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Wanliu Peng Mengyu Gao Xinglong Zhu Xinmei Liu Guang Yang Shun Li Yong Liu Lang Bai Jiayin Yang Ji Bao Source Type: research
A new look at the fluorescent protein-based approach for identifying optimal coding sequence for recombinant protein expression in E. coli
Biotechnol J. 2024 Apr;19(4):e2300343. doi: 10.1002/biot.202300343.ABSTRACTDue to the degeneracy of the genetic code, most amino acids are encoded by several codons. The choice among synonymous codons at the N-terminus of genes has a profound effect on protein expression in Escherichia coli. This is often explained by the different contributions of synonymous codons to mRNA secondary structure formation. Strong secondary structures at the 5'-end of mRNA interfere with ribosome binding and affect the process of translation initiation. In silico optimization of the gene 5'-end can significantly increase the level of protein ...
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Artur I Zabolotskii Natalia S Riabkova Source Type: research
Rainbow screening: Chromoproteins enable visualized molecular cloning
Biotechnol J. 2024 Apr;19(4):e2400114. doi: 10.1002/biot.202400114.ABSTRACTMolecular cloning facilitates the assembly of heterologous DNA fragments with vectors, resulting in the generation of plasmids that can steadily replicate in host cells. To efficiently and accurately screen out the expected plasmid candidates, various methods, such as blue-white screening, have been developed for visualization. However, these methods typically require additional genetic manipulations and costs. To simplify the process of visualized molecular cloning, here we report Rainbow Screening, a method that combines Gibson Assembly with chrom...
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Fang Ba Yufei Zhang Wan-Qiu Liu Jian Li Source Type: research
Fluid shear force and hydrostatic pressure jointly promote osteogenic differentiation of BMSCs by activating YAP1 and NFAT2
This study investigated the impact of FSS and HP on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and examined the distinctions and interactions between the two mechanisms. The results demonstrated that both FSS and HP individually enhanced the osteogenic differentiation of BMSCs, with a more pronounced effect observed through their combined application. BMSCs responded to external FSS and HP stimulation through the integrin-cytoskeleton and Piezo1 ion channel respectively. This led to the activation of downstream biochemical signals, resulting in the dephosphorylation and nuclear translocati...
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Yi Zhou Pan Guo Ziyang Jin Miaomiao Chai Shuhong Zhang Xianwei Wang Wen-Song Tan Yan Zhou Source Type: research
Recent advances in the synthesis of single-stranded DNA in vitro
Biotechnol J. 2024 Apr;19(4):e2400026. doi: 10.1002/biot.202400026.ABSTRACTSingle-stranded DNA (ssDNA) is the foundation of modern biology, with wide applications in gene editing, sequencing, DNA information storage, and materials science. However, synthesizing ssDNA with high efficiency, high throughput, and low error rate in vitro remains a major challenge. Various methods have been developed for ssDNA synthesis, and some significant results have been achieved. In this review, six main methods were introduced, including solid-phase oligonucleotide synthesis, terminal deoxynucleotidyl transferase-based ssDNA synthesis, re...
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Shuai Li Wei Tan Xuemei Jia Qing Miao Ying Liu Dayong Yang Source Type: research
Depolymerization of the polyester-polyurethane by amidase GatA250 and enhancing the production of 4,4'-methylenedianiline with cutinase LCC
This study provides valuable insights into PU pollution control and also proposes applicable solutions to manage PU wastes through bio-recycling.PMID:38622797 | DOI:10.1002/biot.202300723 (Source: Biotechnology Journal)
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Kaiyuan Xin Jianqi Lu Qingyi Zeng Tianyang Zhang Jiawei Liu Jie Zhou Weiliang Dong Min Jiang Source Type: research
Visual screening of CRISPR/Cas9 editing efficiency based on micropattern arrays for editing porcine cells
Biotechnol J. 2024 Apr;19(4):e2300691. doi: 10.1002/biot.202300691.ABSTRACTCRISPR/Cas9 technology, combined with somatic cell nuclear transplantation (SCNT), represents the primary approach to generating gene-edited pigs. The inefficiency in acquiring gene-edited nuclear donors is attributed to low editing and delivery efficiency, both closely linked to the selection of CRISPR/Cas9 forms. However, there is currently no direct method to evaluate the efficiency of CRISPR/Cas9 editing in porcine genomes. A platform based on fluorescence reporting signals and micropattern arrays was developed in this study, to visually assess ...
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Wanliu Peng Mengyu Gao Xinglong Zhu Xinmei Liu Guang Yang Shun Li Yong Liu Lang Bai Jiayin Yang Ji Bao Source Type: research
Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases
Biotechnol J. 2024 Apr;19(4):e2400053. doi: 10.1002/biot.202400053. Epub 2024 Apr 9.ABSTRACTThe rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering re...
Source: Biotechnology Journal - April 9, 2024 Category: Biotechnology Authors: Konstantinos Makryniotis Efstratios Nikolaivits George Taxeidis Jasmina Nikodinovic-Runic Evangelos Topakas Source Type: research
Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases
Biotechnol J. 2024 Apr 9:e2400053. doi: 10.1002/biot.202400053. Online ahead of print.ABSTRACTThe rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering ...
Source: Biotechnology Journal - April 9, 2024 Category: Biotechnology Authors: Konstantinos Makryniotis Efstratios Nikolaivits George Taxeidis Jasmina Nikodinovic-Runic Evangelos Topakas Source Type: research
Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases
Biotechnol J. 2024 Apr 9:e2400053. doi: 10.1002/biot.202400053. Online ahead of print.ABSTRACTThe rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering ...
Source: Biotechnology Journal - April 9, 2024 Category: Biotechnology Authors: Konstantinos Makryniotis Efstratios Nikolaivits George Taxeidis Jasmina Nikodinovic-Runic Evangelos Topakas Source Type: research
