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Editorial Board: Biotechnology Journal 11/2009.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: no abstract. PMID: 19918779 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Biotec Visions 2009, November-December.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: Nobel Prizes 2009: Ribosomes - Telomeres and telomerasesEncyclopaedia of Life Sciences: SNP genotyping technologies - Molecular mimicrySpecial issues: Chinese microbial ecology - Advances in yeast proteomics - MALDI-TOF "Flip-flop" drug susceptibility testNews: Phytophthora infestans genome - Sequencing bacterial transcriptomes - Stem cells from fat - Selecting green clones - Endogenous mutagenic force - Green batteries - Tobacco-produced vaccine - O(2) transport in artificial liver - Endolysins instead of antibiotics - Quick switch key for mitochondria - Climate change shrinks algae - Bacteria degrade microc...
Source: Biotechnology Journal - November 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Special Focus: Nanotechnology and Biochips.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: IntroductionSome nanotech/biochip companiesBiochip product newsBiochips in our sister journals. PMID: 19918781 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Meeting Report: Brain matters - New directions in neuroethics.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
no abstract. PMID: 19918782 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 13, 2009 Category: Biotechnology Authors: Outram S Tags: Biotechnol J Source Type: journals

Editorial: Biochips and nanobiotechnology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
no abstract. PMID: 19918783 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 13, 2009 Category: Biotechnology Authors: Park HG, Lee SY Tags: Biotechnol J Source Type: journals

In this issue: Biotechnology Journal 11/2009.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: FORENSIC IDENTIFICATION ON CHIPS: Choi and Seo et al., Biotechnol. J. 2009, 4, 1530-1541Short tandem repeat (STR) analysis can be used for genetic fingerprinting of individuals as it is done for forensic human identification. However, the current state-of-the-art STR genotyping processes and instruments are labor intensive, expensive, time consuming, and lack portability. Micro-total-analysis systems or lab-on-a-chip platforms based on microfabrication technologies have the capability to miniaturize and integrate bioanalysis steps in a single format and have already been successfully applied for forensic STR ...
Source: Biotechnology Journal - November 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

A-ring ortho-specific monohydroxylation of daidzein by cytochrome P450s of Nocardia farcinica IFM10152.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The bioconversion of the isoflavonoid daidzein using whole cell Nocardia farcinica IFM10152 showed two kinds of major metabolic modifications, i.e. mono-hydroxylation and subsequent O-methylation. The major hydroxylated products of daidzein prior to the O-methylation reaction were 3',4',7-trihydroxyisoflavone (3'-ODI), 4',6,7-trihydroxyisoflavone (6-ODI) and 4',7,8-trihydroxyisoflavone (8-ODI), which are mono-hydroxylated at the ortho position of each hydroxyl group of daidzein. To identify monooxygenases playing a key role in the monohydroxylation of the A-ring of daidzein, all genes of 27 cytochrome P450s from N. far...
Source: Biotechnology Journal - November 13, 2009 Category: Biotechnology Authors: Choi KY, Kim TJ, Koh SK, Roh CH, Pandey BP, Lee N, Kim BG Tags: Biotechnol J Source Type: journals

Surface plasmon resonance for high-throughput ligand screening of membrane-bound proteins.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Technologies based on surface plasmon resonance (SPR) have allowed rapid, label-free characterization of protein-protein and protein-small molecule interactions. SPR has become the gold standard in industrial and academic settings, in which the interaction between a pair of soluble binding partners is characterized in detail or a library of molecules is screened for binding against a single soluble protein. In spite of these successes, SPR is only beginning to be adapted to the needs of membrane-bound proteins which are difficult to study in situ but represent promising targets for drug and biomarker development. Exist...
Source: Biotechnology Journal - November 13, 2009 Category: Biotechnology Authors: Maynard JA, Lindquist NC, Sutherland JN, Lesuffleur A, Warrington AE, Rodriguez M, Oh SH Tags: Biotechnol J Source Type: journals

Novel microfluidic platform for culturing neurons: Culturing and biochemical analysis of neuronal components.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Neurons, one of the most polarized types of cells, are typically composed of cell bodies (soma), dendrites, and axons. Many events such as electric signal transmission, axonal transport, and local protein synthesis occur in the axon, so that a method for isolating axons from somata and dendrites is required for systematically investigating these axonal events. Based on a previously developed neuron culture method for isolating and directing the growth of central nervous system axons without introducing neutrophins, we report three modified microfluidic platforms: (1) for performing biochemical analysis of the pure axon...
Source: Biotechnology Journal - November 13, 2009 Category: Biotechnology Authors: Park JW, Kim HJ, Byun JH, Ryu HR, Jeon NL Tags: Biotechnol J Source Type: journals

Meetings and Conferences: Biotechnology Journal 11/2009.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: no abstract. PMID: 19918788 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Relationship of protozoan biomass to phosphate and nitrate removal from activated sludge mixed liquor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The relationship between protozoan biomass concentration and phosphate and nitrate removal was investigated in mixed liquor using three different carbon sources as supplements. The study was carried out using three respective initial biomass concentrations in a shaking flask environment. Samples were taken every 24 h to determine phosphate, nitrate, dissolved oxygen and chemical oxygen demand. The results revealed a direct relationship between decreases in nutrient concentrations and increases in cell densities of the isolates. Between 24 and 96 h, the increases in the protozoan density corresponded to a phosphate decr...
Source: Biotechnology Journal - November 9, 2009 Category: Biotechnology Authors: Akpor OB, Momba MN Tags: Biotechnol J Source Type: journals

Recent trends and some applications of isothermal titration calorimetry in biotechnology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Isothermal titration calorimeters (ITCs) are thermodynamic instruments used for the determination of enthalpy changes in any physical/chemical reaction. This can be applied in various fields of biotechnology. This review explains ITC applications, especially in bioseparation, drug development and cell metabolism. In liquid chromatography, the separation/purification of specific proteins or polypeptides in a mixture is usually achieved by varying the adsorption affinities of the different proteins/polypeptides for the adsorbent under different mobile-phase conditions and temperatures. Using ITC analysis, the binding mec...
Source: Biotechnology Journal - November 9, 2009 Category: Biotechnology Authors: Roselin LS, Lin MS, Lin PH, Chang Y, Chen WY Tags: Biotechnol J Source Type: journals

A food safety control low mass-range proteomics platform for the detection of illicit treatments in veal calves by MALDI-TOF-MS serum profiling.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Performance enhancing agents (PEAs) are illegally used in cattle and other meat producing species to increase food conversion and lean meat production. Due to the very short breeding cycle, veal calves represent the meat producing bovine category mostly subjected to illicit treatments. These chemical agents are difficult to detect by conventional analytical approaches due to the employment of synergistic formulations at very low dosage and given the use of uncharacterized novel compounds. Such a scenario has fostered a strong interest in the discovery of functional molecular biomarkers for the detection of growth promo...
Source: Biotechnology Journal - October 19, 2009 Category: Biotechnology Authors: Della Donna L, Ronci M, Sacchetta P, Di Ilio C, Biolatti B, Federici G, Nebbia C, Urbani A Tags: Biotechnol J Source Type: journals

Barley as a green factory for the production of functional Flt3 ligand.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Biologically active recombinant human Flt3 ligand was expressed and isolated from transgenic barley seeds. Its expression is controlled by a tissue specific promoter that confines accumulation of the recombinant protein to the endosperm tissue of the seed. The recombinant Flt3 ligand variant expressed in the seeds contains an HQ-tag for affinity purification on immobilized metal ion affinity chromatography (IMAC) resin. The tagged protein was purified from seed extracts to near homogeneity using sequential chromatography on IMAC affinity resin and cation exchange resin. We also show that the recombinant Flt3 ligand pro...
Source: Biotechnology Journal - October 19, 2009 Category: Biotechnology Authors: Erlendsson LS, Muench MO, Hellman U, Hrafnkelsdóttir SM, Jonsson A, Balmer Y, Mäntylä E, Orvar BL Tags: Biotechnol J Source Type: journals

Insect-symbiont systems: From complex relationships to biotechnological applications.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Microbial symbiosis is a ubiquitous aspect of life and was a major element in the ability of insects to explore several adverse environments. To date, the study of symbiosis in insects has been impaired by the unculturability of most symbionts. However, some molecular methods represent powerful tools to help understand insect-microorganism associations and to disclose new symbiont-host systems. Beyond playing an essential role in nutrition and development of the insects, symbionts can produce bioactive compounds that protect the host against adverse environmental conditions, predators and/or direct competitors. Since t...
Source: Biotechnology Journal - October 19, 2009 Category: Biotechnology Authors: Chaves S, Neto M, Tenreiro R Tags: Biotechnol J Source Type: journals

An integrated microdevice for high-performance short tandem repeat genotyping.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Short tandem repeat (STR) analysis provides genetic fingerprinting of individuals, and is considered as a powerful and indispensable technique for forensic human identification. However, the current state-of-the-art STR genotyping processes and instruments are labor intensive, expensive, time consuming, and lack portability. Micro-total-analysis systems or lab-on-a-chip platforms based on microfabrication technologies have the capability to miniaturize and integrate bioanalysis steps in a single format. Recent progress in microsystems has demonstrated their successful performance for the forensic STR typing with a redu...
Source: Biotechnology Journal - October 19, 2009 Category: Biotechnology Authors: Choi JY, Seo TS Tags: Biotechnol J Source Type: journals

Industrial biotechnology: Tools and applications.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Industrial biotechnology involves the use of enzymes and microorganisms to produce value-added chemicals from renewable sources. Because of its association with reduced energy consumption, greenhouse gas emissions, and waste generation, industrial biotechnology is a rapidly growing field. Here we highlight a variety of important tools for industrial biotechnology, including protein engineering, metabolic engineering, synthetic biology, systems biology, and downstream processing. In addition, we show how these tools have been successfully applied in several case studies, including the production of 1, 3-propanediol, lac...
Source: Biotechnology Journal - October 19, 2009 Category: Biotechnology Authors: Tang WL, Zhao H Tags: Biotechnol J Source Type: journals

Biosynthesis of Sb(2)O(3) nanoparticles: A low-cost green approach.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A low-cost green and reproducible microbe (Lactobacillus sp.)-mediated biosynthesis of Sb(2)O(3) nanoparticles is reported. The synthesis was performed at around room temperature. X-ray and transmission electron microscopy analyses were performed to ascertain the formation of Sb(2)O(3) nanoparticles. X-ray analysis indicated that Sb(2)O(3) nanoparticles had a face-centered cubic unit cell structure. Individual nanoparticles as well as a few aggregates of 3-12 nm were found. A possible mechanism for the synthesis of nano Sb(2)O(3) is proposed. PMID: 19844916 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 19, 2009 Category: Biotechnology Authors: Jha AK, Prasad K, Prasad K Tags: Biotechnol J Source Type: journals

Statistical aspects of design and validation of microtitre-plate-based linear and non-linear parallel in vitro bioassays.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models. PMID: 1...
Source: Biotechnology Journal - October 19, 2009 Category: Biotechnology Authors: Zimmermann H, Gerhard D, Dingermann T, Hothorn LA Tags: Biotechnol J Source Type: journals

Recent trends in non-viral vector-mediated gene delivery.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nucleic acids-based next generation biopharmaceuticals (i.e., pDNA, oligonucleotides, short interfering RNA) are potential pioneering materials to cope with various incurable diseases. However, several biological barriers present a challenge for efficient gene delivery. On the other hand, developments in nanotechnology now offer numerous non-viral vectors that have been fabricated and found capable of transmitting the biopharmaceuticals into the cell and even into specific subcellular compartments like mitochondria. This overview illustrates cellular barriers and current status of non-viral gene vectors, i.e., lipoplex...
Source: Biotechnology Journal - October 19, 2009 Category: Biotechnology Authors: Pathak A, Patnaik S, Gupta KC Tags: Biotechnol J Source Type: journals

Chemical strategies for immobilization of oligonucleotides.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The development of oligonucleotide-based microarrays (biochips) is a major thrust area in the rapidly growing biotechnology industry, which encompasses a diverse range of research areas including genomics, proteomics, computational biology, and pharmaceuticals, among other activities. Microarray experiments have proved to be unique in offering cost-effective and efficient analysis at the genomic level. In the last few years, biochips have gained increasing acceptance in the study of genetic and cellular processes. As the increase in experimental throughput has posed many challenges to the research community, considerab...
Source: Biotechnology Journal - October 19, 2009 Category: Biotechnology Authors: Sethi D, Gandhi RP, Kuma P, Gupta KC Tags: Biotechnol J Source Type: journals

Focus: Synthetic Biology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: Cover illustrationFocus on synthetic biology. This special issue is edited by Kristala Jones Prather and Alfonso Jaramillo and focuses on computational methods, modeling and applications of synthetic biology. Bottom right: Penicillum simplicissimum in solid-state fermentation producing lipases (2000x magnification by Dr. Ulysses Lins, Microbiology Institute-UFRJ, Brazil), see article by Gutarra et al. on p. 1450 of this issue (http://dx.doi.org/10.1002/biot.200800298). Other images: circuit board and gears (both (c) Getty images). PMID: 19830707 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Editorial: Focus on synthetic biology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
no abstract. PMID: 19830708 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Authors: Prather KL, Jaramillo A Tags: Biotechnol J Source Type: journals

In this issue: Biotechnology Journal 10/2009.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: SYNTHETIC BIOLOGY - COMPUTATIONAL METHODS: Kaznessis et al., Biotechnol. J. 2009, 4, 1392-1405Professor Yiannis Kaznessis from Minnesota (MN, USA) discusses how theoretical synthetic biology may liberate empiricism in biological sciences beyond the unaided human brain. Because synthetic biological systems are relatively small and largely independent of evolutionary contexts, they can be represented with mathematical models strongly founded on first principles of molecular biology and laws of statistical thermodynamics. This may then be plausibly used to explain in unambiguous, quantitative terms how biologica...
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Editorial Board: Biotechnology Journal 10/2009.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: no abstract. PMID: 19830710 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Special focus: Synthetic biology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: Special focus: Synthetic biologyWhat is synthetic biology?SynBERC - The Synthetic Biology Engineering Research CenterArs SyntheticaiGEM - The International Genetically Engineered Machine competitionSome synthetic biology companiesPaper watch: Synthetic biologyBuilding blocks for novel functionsKnowledge-making distinctions in synthetic biologyScaffold design and manufacturing: From concept to clinicPeptidomimetics - a versatile route to biologically active compoundsMetabolic engineering of E. coliE. coli needs safety valvesSystems-level metabolic engineeringMammalian synthetic biologyChemical aspects of synth...
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Biotech round the world: Kenya.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: Facts and figuresAgriculture and green biotechnology in KenyaJomo Kenyatta University of Agriculture and TechnologyRed biotechnology in Kenya. PMID: 19830712 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

(Re-)construction, characterization and modeling of systems for synthetic biology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
no abstract. PMID: 19830713 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Authors: Diez MS, Lam CM, Leprince A, Martins Dos Santos VA Tags: Biotechnol J Source Type: journals

Computational methods in synthetic biology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We discuss how a theoretical synthetic biology research programme may liberate empiricism in biological sciences beyond the unaided human brain. Because synthetic biological systems are relatively small and largely independent of evolutionary contexts, they can be represented with mathematical models strongly founded on first principles of molecular biology and laws of statistical thermodynamics. A universal mathematical formalism for describing synthetic constructs may then be plausibly used to explain in unambiguous, quantitative terms how biological phenotypic complexity emerges as a result of well-defined biomolecu...
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Authors: Kaznessis YN Tags: Biotechnol J Source Type: journals

Practical application of synthetic biology principles.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Synthetic biology can be defined as the "repurposing and redesign of biological systems for novel purposes or applications, " and the field lies at the interface of several biological research areas. This broad definition can be taken to include a variety of investigative endeavors, and successful design of new biological paradigms requires integration of many scientific disciplines including (but not limited to) protein engineering, metabolic engineering, genomics, structural biology, chemical biology, systems biology, and bioinformatics. This review focuses on recent applications of synthetic biology principles in th...
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Authors: Marner WD Tags: Biotechnol J Source Type: journals

Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To improve butanol selectivity, Clostridium acetobutylicum M5(pIMP1E1AB) was constructed by adhE1-ctfAB complementation of C. acetobutylicum M5, a derivative strain of C. acetobutylicum ATCC 824, which does not produce solvents due to the lack of megaplasmid pSOL1. The gene products of adhE1-ctfAB catalyze the formation of acetoacetate and ethanol/butanol with acid re-assimilation in solventogenesis. Effects of the adhE1-ctfAB complementation of M5 were studied by batch fermentations under various pH and glucose concentrations, and by flux balance analysis using a genome-scale metabolic model for this organism. The met...
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Authors: Lee JY, Jang YS, Lee J, Papoutsakis ET, Lee SY Tags: Biotechnol J Source Type: journals

Electroporation optimization to deliver plasmid DNA into dental follicle cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Electroporation is a simple and versatile approach for DNA transfer but needs to be optimized for specific cells. We conducted square wave electroporation experiments for rat dental follicle cells under various conditions. These experiments indicated that the optimal electroporation electric field strength was 375 V/cm, and that plasmid concentrations greater than 0.18 mug/muL were required to achieve high transfection efficiency. BSA or fetal bovine serum in the pulsing buffer significantly improved cell survival and increased the number of transfected cells. The optimal pulsing duration was in the range of 45-120 ms ...
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Authors: Yao S, Rana S, Liu D, Wise GE Tags: Biotechnol J Source Type: journals

Meetings and Conferences: Biotechnology Journal 10/2009.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: no abstract. PMID: 19830718 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

In vitro liver model using microfabricated scaffolds in a modular bioreactor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hepatocyte function on 3-D microfabricated polymer scaffolds realised with the pressure-activated microsyringe was tested under static and dynamic conditions. The dynamic cell culture was obtained using the multicompartment modular bioreactor system. Hepatocyte cell density, glucose consumption, and albumin secretion rate were measured daily over a week. Cells seeded on scaffolds showed an increase in cell density compared with monolayer controls. Moreover, in dynamic culture, cell metabolic function increased three times in comparison with static monolayer cultures. These results suggest that cell density and cell-cel...
Source: Biotechnology Journal - October 11, 2009 Category: Biotechnology Authors: Vinci B, Cavallone D, Vozzi G, Mazzei D, Domenici C, Brunetto M, Ahluwalia A Tags: Biotechnol J Source Type: journals

Rabies virus glycoprotein expression in Drosophila S2 cells: Influence of re-selection on protein expression.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study was to achieve expression of recombinant rabies virus glycoprotein (rRVGP) in Drosophila S2 cells. For this, a cDNA coding for the selection hygromycin antibiotic and the cDNA encoding the RVGP protein under the control of the constitutive actin promoter (Ac) were cloned in an expression plasmid, which was transfected into S2 cells. S2 cell populations (S2AcRVGPHy) showed rRVGP expression in cell lysates, attaining concentrations up to 1.5 mug/10(7) cells (705 mug/L). Of the transfected cells, 20% were shown to express the rRVGP. Cell subpopulations selected by limiting dilution expressed higher r...
Source: Biotechnology Journal - October 11, 2009 Category: Biotechnology Authors: Dos Santos AS, Lemos MA, Pereira CA, Jorge SA Tags: Biotechnol J Source Type: journals

Sampling methods for NMR-based metabolomics of Staphylococcus aureus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To select an appropriate sampling method for comparison of metabolite profiles between planktonic and biofilm Staphylococcus aureus using NMR techniques, we evaluated three methods: quenching-centrifugation (QC), filtration-quenching (FQ) and filtration-quenching-lyophilization (FQL). We found differences in metabolite loss, yield, reproducibility and metabolite profile. QC caused severe metabolite leakage and possible decomposition of nucleotides. FQ achieved high yields and reproducibility, although it had the disadvantages of long filtration and rinse times before quenching. FQL resulted in a loss of a few metabolit...
Source: Biotechnology Journal - October 11, 2009 Category: Biotechnology Authors: Wu XH, Yu HL, Ba ZY, Chen JY, Sun HG, Han BZ Tags: Biotechnol J Source Type: journals

Editorial Board: Biotechnology Journal 9/2009.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: no abstract. PMID: 19777475 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - September 22, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Biotec Visions September 2009.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: News: Fighting iron deficiency - Measuring cellular forces - Better photobioreactor design for hydrogen production - Artificial nerve cell - After GFP: Infrared IFP - Predictable transfection - RNAi for improved protein production - 3D protein map - Self-irrigating rhubarb - Cool plasma to fight biofilms - Pus elasticity - Plants surviving the frostSpecial Issues: Imaging host-pathogen interactions - Plant in vitro technologies - BiodieselWIREs Nanomedicine and Nanobiotechnology: Drug-eluting stent coatings - Particle fabrication for diagnostic imaging and drug deliveryEncyclopaedia of Life Sciences: Antiprot...
Source: Biotechnology Journal - September 22, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Methods and Advances.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: Cover illustration: Methods and Advances in Biotech. Cover images depict tools for metabolite analysis in biocatalytic synthesis (see p. 1253). Direct infusion mass spectrometry and LC-MS (bottom) have been particularly useful for the rapid analysis of complex metabolites, derivatives and homologues. The complementary separation power of capillary electrophoresis (middle) combined with sensitive detection is very useful for the analysis of charged metabolites. Chromatographic techniques like GC, LC and TLC are the true workhorses for all classes of metabolites and advanced separations using high-performance l...
Source: Biotechnology Journal - September 22, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Editorial: Methods and Advances in Biotech.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
no abstract. PMID: 19777478 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - September 22, 2009 Category: Biotechnology Authors: Lee SY Tags: Biotechnol J Source Type: journals

In this issue: Biotechnology Journal 9/2009.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: USING PLANT HORMONES IN BIOTECHNOLOGY: Csukasi et al., Biotechnol. J. 2009, 4, 1293-130Plant hormones have determinant roles in the final value of agricultural products since plant domestication. Therefore, advances in deciphering their structure, synthesis, perception and signaling pathways have been a main focus for research. Application of basic information on plant hormones in food quality and production has established some landmarks in plant biotechnology, e.g. from the dramatic increase in rice yield to long life tomatoes. The review presented by Victoriano Valpuesta and colleagues from Málaga, Sp...
Source: Biotechnology Journal - September 22, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Biotech news and views.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: Biotech news and views:Up-scaled vaccine productionBiodiesel synthesis by biotechnological methodsBiological particles in ice cloudsMicroorganisms producing nanoparticlesNanotechnology boost: MIT-INLBook highlight:Systems biology and biotechnology of Escherichia coliMost accessed articles in BTJ:Bacteria biosensorsTomography of malaria parasitesNew scaffold for artificial skinNew on the market:Millipore's disposable bioreactorOlympus' all-in-one microscope familyEngineering in Life Sciences:Increasing your yieldsBiosensing ethylene dibromideModeling cellulose formationBioEssays highlights:SNPing the RNAi conn...
Source: Biotechnology Journal - September 22, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Meeting preview: APBioChEC'09.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: no abstract. PMID: 19777481 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - September 22, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Biotech round the world: Australia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: Facts and FiguresRegulation of research in AustraliaGreen biotechnologySome Australian biotech companies. PMID: 19777482 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - September 22, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Tools and ingredients for the biocatalytic synthesis of metabolites.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Metabolic networks have been an interesting starting point not only for the design of synthetic routes in a similar sequence of reactions, e.g., in biomimetic syntheses, but also for assembling a number of biocatalytic steps by preparing the required enzymes and auxiliary reagents. Retrosynthetic analysis involving multiple biocatalytic reactions steps therefore needs to consider the practically realized biocatalytic single steps. The opportunities for route selection are enlarged if novel synthetic reactions connecting easily available starting materials and products are found, and/or both biocatalytic and classical r...
Source: Biotechnology Journal - September 22, 2009 Category: Biotechnology Authors: Wohlgemuth R Tags: Biotechnol J Source Type: journals

Single cell analysis: Technologies and applications. By Dario Anselmetti (Ed.).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
no abstract. PMID: 19777484 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - September 22, 2009 Category: Biotechnology Authors: Kasper C Tags: Biotechnol J Source Type: journals

Meetings and Conferences: Biotechnology Journal 9/2009.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: no abstract. PMID: 19777485 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - September 22, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals

Biocatalytic asymmetric amination of carbonyl functional groups - a synthetic biology approach to organic chemistry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Transaminases catalyze amino transfer reactions from amino donors such as amino acids or amines to keto acids or ketones to give chiral amino acid or amines in optically pure form. alpha-Amino acid dehydrogenases catalyze the asymmetric reductive amination of alpha-keto acids using ammonia as amino donor to furnish L-amino acids. The distinct features and synthetic application of these two enzymes are reviewed in an effort to illustrate their promising and challenging aspects in serving as approaches to the direct asymmetric synthesis of optically pure amines from the corresponding keto compounds, a formidable problem ...
Source: Biotechnology Journal - September 15, 2009 Category: Biotechnology Authors: Zhu D, Hua L Tags: Biotechnol J Source Type: journals

A GFP-based method facilitates clonal selection of transfected CHO cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The identification of highly expressing clones is a crucial step in the development of cell lines for production of recombinant proteins. Here we present a method based on the co-expression of enhanced green fluorescent protein (EGFP) that allows clonal selection in standard 96-well cell culture plates. The genes encoding the EGFP protein and the protein of interest are linked by an internal ribosome entry site and thus are transcribed into the same mRNA but are translated independently. Since both proteins arise from a common mRNA, the EGFP expression level correlates with the expression level of the therapeutic prote...
Source: Biotechnology Journal - September 1, 2009 Category: Biotechnology Authors: Freimark D, Jèrôme V, Freitag R Tags: Biotechnol J Source Type: journals

Biotech in the financial crisis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: Cover illustration: Biotech in the financial crisis. This special issue of Biotechnology Journal covers how the current financial turmoil creates problems but also chances for biotech. Forum articles and biotech highlights ask whether biotech can rescue economy and environment (green growth). Image (c) PhotoDisc, Inc. PMID: 19685455 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - August 13, 2009 Category: Biotechnology Tags: Biotechnol J Source Type: journals