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Correlation Between Flagellin A (flaA) Genotypes and Antimicrobial Susceptibility Patterns of Campylobacter jejuni Strains Isolated from Children with Gastroenteritis in Athens, GreeceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Molecular Diagnosis)
Source: Molecular Diagnosis - November 21, 2009 Category: Pathology Authors: Ioannidis, AnastassiosNicolaou, ChrysoulaChatzipanagiotou, Stylianos Tags: Short Communications Source Type: journals

[Growth characteristics of Campylobacter Jejuni on different culture media and their expression of outer membrane proteins.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSION: Improved Bull's medium yolk agar allows rapid growth of CJ with typical biological characteristics and enhanced expression of the OMPs with molecular weight of 28 -31 kD, and can be widely used in CJ subunit vaccine development, CJ epidemiological survey, CJ food safety examination, and CJ quarantine. PMID: 19923087 [PubMed - as supplied by publisher] (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - November 20, 2009 Category: Universities & Medical Training Authors: Feng SJ, Wu LD, Sun WB, Xiao Z, Mi N, Liu F, Chen Q Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: journals

Inflammatory Peripheral Neuropathies Prevalent in Poultry and Swine FarmersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Symptoms of Guillain-Barré syndrome resulting from infection with Campylobacter jejuni, a common pathogen of poultry and swine, are prevalent in farm workers with high exposure to these animals. Medscape Medical News (Source: Medscape Today Headlines)
Source: Medscape Today Headlines - November 18, 2009 Category: Consumer Health News Tags: Public Health & Prevention Source Type: news

Recrudescent Campylobacter jejuni infection in an immunocompetent adult following experimental infection with a well characterized organism.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present the complete clinical, microbiologic and immunologic evaluation of a closely-monitored healthy male with recrudescent C. jejuni infection following experimental infection with a well characterized strain and occurring in the absence of immunodeficiency. Post-antibiotics, initial infection was clinically cleared and microbiologically undetectable. Subsequently, two episodes of recrudescence occurred, with no change in in vitro antibiotic sensitivity. Immune responses were compared to other participants in the experimental infection: innate immune responses, including fecal cytokines and C-reactive protein were in...
Source: Clinical and Vaccine Immunology - November 18, 2009 Category: Allergy & Immunology Authors: Baqar S, Tribble DR, Carmolli M, Sadigh K, Poly F, Porter C, Larsson CJ, Pierce KK, Guerry P, , Darsley M, Kirkpatrick B Tags: Clin Vaccine Immunol Source Type: journals

Prevalence of Diarrhea and Enteropathogens in Racing Sled DogsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Diarrhea is highly prevalent in racing sled dogs, although the underlying causes are poorly understood. Clostridium perfringens enterotoxin (CPE) and Clostridium difficile Toxin A and B are associated with diarrhea in racing sled dogs. One hundred and thirty-five sled dogs. Freshly voided feces were obtained from 55 dogs before racing and from 80 dogs after 400 miles of racing. Samples were visually scored for diarrhea, mucus, blood, and melena. CPE and C. difficile Toxin A and B were detected by ELISA. Samples were cultured for C. perfringens, C. difficile, Campylobacter, Salmonella, and Escherichia coli 0157; Giardia and...
Source: Journal of Veterinary Internal Medicine - November 17, 2009 Category: Veterinary Research Authors: E. McKenzie, J. Riehl, H. Banse, P.H. Kass, S. Nelson, Jr, S.L. Marks Source Type: journals

NMR Spectroscopic Characterization of the Sialyltransferase CstII from Campylobacter jejuni: Histidine 188 Is the General BaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 6, 2009 Category: Biochemistry Tags: article Source Type: journals

Campylobacter concisus and other Campylobacter species in children with newly diagnosed Crohn's diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Campylobacter concisus and other members of the Campylobacter genus have recently been suggested as possible etiological agents of Crohn's disease (CD). To further investigate this issue we determined the prevalence of these organisms in pediatric patients newly diagnosed with CD.DNA was extracted from fecal specimens collected from 54 children with CD, 27 noninflammatory bowel disease (non-IBD), and 33 healthy controls and subjected to polymerase chain reaction (PCR) sequencing.Detection of C. concisus DNA using a newly developed PCR assay targeting the 16S rRNA gene of C. concisus showed that 65% (35/54) of fecal samples...
Source: Inflammatory Bowel Diseases - November 2, 2009 Category: Gastroenterology Authors: Si Ming Man, Li Zhang, Andrew S. Day, Steven T. Leach, Daniel A. Lemberg, Hazel Mitchell Source Type: journals

Effect of storage regime on the stability of DNA used as a calibration standard for real-time polymerase chain reaction.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This article looks at storage factors influencing the stability of potential DNA calibration standards for use in quantitative polymerase chain reaction (PCR). Target sequences from the bacteria Campylobacter jejuni were cloned into a plasmid vector. Samples of these potential calibration standards were stored at +4, -20, and -80 degrees C as aqueous and lyophilized samples and were prepared as both single-use aliquots and multiple-use preparations. Results showed that the samples stored as single-use aqueous solutions at +4 degrees C and lyophilized samples stored at +4 and -20 degrees C were the most stable. Samples stor...
Source: Analytical Biochemistry - November 1, 2009 Category: Biochemistry Authors: Podivinsky E, Love JL, van der Colff L, Samuel L Tags: Anal Biochem Source Type: journals

Bacterial Contamination of Hands Increases Risk of Cross-contamination among Low-income Puerto Rican Meal PreparersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions and Implications: Meal preparer's hands can be a vehicle of pathogen transmission during meal preparation. (Source: Journal of Nutrition Education and Behavior)
Source: Journal of Nutrition Education and Behavior - October 31, 2009 Category: Nutrition Authors: Jigna Morarji Dharod, Stefania Paciello, Angela Bermúdez-Millán, Kumar Venkitanarayanan, Grace Damio, Rafael Pérez-Escamilla Tags: Research Articles Source Type: journals

Metabolomics in GlycomicsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Metabolomics is essentially the study of all low molecular weight molecules in a biological system under defined conditions. In glycomics, there is much potential to gain insight into the biosynthesis of novel glycoconjugate structures by probing the metabolome for substrates that are suspected, or known, to be involved in the biosynthetic processes. Recently, we employed the use of hydrophilic interaction liquid chromatography–mass spectrometry (HILIC–MS) in a focused metabolomic study of sugar-nucleotides relevant to the biosynthesis of highly novel carbohydrate modifications on the flagellin of Campylobacter...
Source: Springer protocols feed by Biochemistry - October 30, 2009 Category: Biochemistry Source Type: info

Characterization of Lipid-Linked Oligosaccharides by Mass SpectrometryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
N- Glycosylation of proteins is recognized as one of the most common post-translational modifications. Until recently it was believed that N-glycosylation occurred exclusively in eukaryotes until the discovery of the general protein glycosylation pathway (Pgl) in Campylobacter jejuni. We have developed a new glycomics strategy based on lectin-affinity capture of lipid-linked oligosaccharides (LLOs) coupled to capillary electrophoresis mass spectrometry. The LLO intermediates of the C. jejuni Pgl pathway were used to validate the methodology and to better characterize the bacterial model system for protein N-glycosylation. ...
Source: Springer protocols feed by Biochemistry - October 30, 2009 Category: Biochemistry Source Type: info

A role for Campylobacter jejuni-induced enteritis in inflammatory bowel disease?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The inflammatory bowel diseases (IBD), Crohn's disease and ulcerative colitis, are T cell-mediated diseases that are characterized by chronic, relapsing inflammation of the intestinal tract. The pathogenesis of IBD involves the complex interaction between the intestinal microflora, host genetic and immune factors, and environmental stimuli. Epidemiological analyses have implicated acute bacterial enteritis as one of the factors that may incite or exacerbate IBD in susceptible individuals. In this review, we examine how interactions between the common enteric pathogen Campylobacter jejuni, the host intestinal epithelium...
Source: Am J Physiol Gastroi... - October 29, 2009 Category: Gastroenterology Authors: Kalischuk LD, Buret AG Tags: Am J Physiol Gastrointest Liver Physiol Source Type: journals

Cytomegalovirus retinitis associated with Good's syndrome.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. CMV retinitis can be associated with Good’s syndrome. PMID: 19882506 [PubMed - as supplied by publisher] (Source: European Journal of Ophthalmology)
Source: European Journal of Ophthalmology - October 28, 2009 Category: Opthalmology Authors: Mateo-Montoya A, Stanescu D, Wolff B, Sahel JA, Bonnel S Tags: Eur J Ophthalmol Source Type: journals

Microbiologic changes in subgingival plaque after removal of fixed orthodontic appliances.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Periodontopathogens during orthodontic treatment were significantly reduced within 3 months of appliance removal. However, how long it takes to return to the preorthodontic composition of the subgingival microbiota and whether it happens at all remain to be seen. PMID: 19852608 [PubMed - in process] (Source: The Angle Orthodontist)
Source: The Angle Orthodontist - October 27, 2009 Category: Dentistry Authors: Choi DS, Cha BK, Jost-Brinkmann PG, Lee SY, Chang BS, Jang I, Song JS Tags: Angle Orthod Source Type: journals

Age‐Related Susceptibility to Infection with Diarrheagenic Escherichia coli among Infants from Periurban Areas in Lima, PeruEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Infectious Diseases, Volume 0, Issue 0, Page 000, Latest Articles. Background. Diarrheagenic Escherichia coli strains are being recognized as important pediatric enteropathogens worldwide. However, it is unclear whether there are differences in age‐related susceptibility to specific strains, especially among infants. Methods. We conducted a passive surveillance cohort study of diarrhea that involved 1034 children aged 2–12 months in Lima, Peru. Control stool samples were collected from randomly selected children without diarrhea. All samples were analyzed for common enteric pathogens and for diarrh...
Source: Clinical Infectious Diseases Latest Issue - October 27, 2009 Category: Infectious Diseases Tags: article MAJOR ARTICLE Source Type: journals

23S rRNA Mutation A2074C Conferring High-Level Macrolide Resistance and Fitness Cost in Campylobacter jejuniEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Microbial Drug Resistance Dec 2009, Vol. 15, No. 4: 239-244. (Source: Microbial Drug Resistance)
Source: Microbial Drug Resistance - October 26, 2009 Category: Microbiology Tags: article Source Type: journals

Antimicrobial Activity of Two Propolis Samples Against Human Campylobacter jejuniEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Journal of Medicinal Food Oct 2009, Vol. 12, No. 5: 1050-1056. (Source: Journal of Medicinal Food)
Source: Journal of Medicinal Food - October 26, 2009 Category: Nutrition Tags: article Source Type: journals

Toward the Use of Genomics to Study Microevolutionary Change in BacteriaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bacteria evolve rapidly in response to the environment they encounter. Some environmental changes are experienced numerous times by bacteria from the same population, providing an opportunity to dissect the genetic basis of adaptive evolution. Here I discuss two examples in which the patterns of rapid change provide insight into medically important bacterial phenotypes, namely immune escape by Neisseria meningitidis and host specificity of Campylobacter jejuni. Genomic analysis of populations of bacteria from these species holds great promise but requires appropriate concepts and statistical tools. (Source: PLoS Genetics)
Source: PLoS Genetics - October 26, 2009 Category: Genetics & Stem Cells Authors: Daniel Falush Source Type: journals

Comparison of basal broth media for the optimal laboratory recovery ofCampylobacter jejuni andCampylobacter coliEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion These studies support laboratory employment of either BHIYE or TSBYE for optimal recovery ofC. jejuni andC. coli and avoidance of nutrient broth. Content Type Journal ArticleCategory Original PaperDOI 10.1007/BF03167693Authors J. E. Moore, Belfast City Hospital Northern Ireland Public Health Laboratory, Department of Bacteriology BT9 7AD Belfast Northern Ireland Journal Irish Journal of Medical ScienceOnline ISSN 1863-4362Print ISSN 0021-1265 Journal Volume Volume 169 Journal Issue Volume 169, Number 3 / July, 2000 (Source: Irish Journal of Medical Science)
Source: Irish Journal of Medical Science - October 23, 2009 Category: Journals (General) Tags: Irish Journal of Medical Science Source Type: journals

Investigation of food and environmental exposures relating to epidemiology of Campylobacter coli in humans in North West England.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
DISCUSSION: there is little evidence to suggest that epidemiological differences between human cases of C. coli and C. jejuni are a result of different food or behavioural exposures alone. PMID: 19854914 [PubMed - as supplied by publisher] (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - October 23, 2009 Category: Microbiology Authors: Sopwith W, Birtles A, Matthews M, Fox A, Gee S, James S, Kempster J, Painter M, Edwards-Jones V, Osborn K, Regan M, Syed Q, Bolton E Tags: Appl Environ Microbiol Source Type: journals

The hrcA and hspR regulons of Campylobacter jejuni.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The human pathogen Campylobacter jejuni has a classic heat shock response, showing induction of chaperones and proteases plus several unidentified proteins in response to small increase in growth temperature. The genome contains two homologues to known heat shock response regulators, HrcA and HspR. Previous work has shown that HspR controls several heat shock genes, but the hrcA regulon has not been defined. We have constructed single and double deletions of C. jejuni hrcA and hspR and analysed gene expression using microarrays. Only a small number of genes are controlled by these two regulators, and the two regulons o...
Source: Microbiology - October 22, 2009 Category: Microbiology Authors: Holmes CW, Penn CW, Lund PA Tags: Microbiology Source Type: journals

Inhibition by Lactobacillus sakei of other species in the flora of vacuum packaged raw meats during prolonged storage.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The abilities of five Lactobacillus sakei strains and one Lactococcus lactis strain to retain inhibitory activity against several target organisms in the flora of product during 12 weeks storage of vacuum-packaged lamb and beef was investigated. L. sakei strains were generally found capable of developing dominant populations on both beef and lamb. L. lactis 75 grew poorly on lamb did not inhibit co-inoculated Brochothrix thermosphacta. Lamb inoculated with the Sakacin-A producer L. sakei Lb706 had lower Listeria monocytogenes populations than lamb inoculated with a bacteriocin-negative variant. In beef packs inoculated...
Source: Food Microbiology - October 21, 2009 Category: Food Science Authors: Jones RJ, Zagorec M, Brightwell G, Tagg JR Tags: Food Microbiol Source Type: journals

Resistance of Listeria monocytogenes, Escherichia coli O157:H7 and Campylobacter jejuni after exposure to repetitive cycles of mild bactericidal treatments.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
While maintaining nutritional and sensorial attributes of fresh foods mild processing technologies generally deliver microbiologically perishable food products. Currently little information exists on possible increase in the resistance of pathogens after repetitive exposure to mild (sub-lethal) treatments. Multiple strain-cocktails of Listeria monocytogenes, Escherichia coli O157:H7 and Campylobacter jejuni were exposed to 20 consecutive cycles of sub-lethal inactivation by three different techniques. Used techniques comprised inactivation with lactic acid (LA), chlorine dioxide (ClO(2)) and intense light pulses (ILP)....
Source: Food Microbiology - October 21, 2009 Category: Food Science Authors: Rajkovic A, Smigic N, Uyttendaele M, Medic H, de Zutter L, Devlieghere F Tags: Food Microbiol Source Type: journals

Campylobacter jejuni Strain CG8421: A Refined Model for the Study of Campylobacteriosis and Evaluation of Campylobacter Vaccines in Human SubjectsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. The C. jejuni CG8421 challenge model provides a safe and effective tool, without the risk of Guillain‐Barré syndrome. The model demonstrates high attack rates after lower doses of challenge inoculum, provides further understanding of immunologic responses, and permits future investigation of candidate Campylobacter vaccines. (Source: Clinical Infectious Diseases Latest Issue)
Source: Clinical Infectious Diseases Latest Issue - October 20, 2009 Category: Infectious Diseases Tags: article MAJOR ARTICLE Source Type: journals

Campylobacter Excreted into the Environment by Animal Sources: Prevalence, Concentration Shed, and Host AssociationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Foodborne Pathogens and Disease , Vol. 0, No. 0. (Source: Foodborne Pathogens and Disease)
Source: Foodborne Pathogens and Disease - October 20, 2009 Category: Food Science Tags: article Source Type: journals

Characterization of the oxidative stress stimulon and PerR regulon of Campylobacter jejuniEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This study reveals an interplay between PerR, Fur, iron metabolism and oxidative stress defense, and highlights the role of these elements in C. jejuni colonization of the chick cecum and/or subsequent survival. (Source: BMC Genomics - Latest articles)
Source: BMC Genomics - Latest articles - October 17, 2009 Category: Genetics & Stem Cells Authors: Kiran PalyadaYi-Qian SunAnnika FlintJames ButcherHemant NaikareAlain Stintzi Source Type: journals

Microbial induction of inflammatory bowel disease associated gene TL1A (TNFSF15) in antigen presenting cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we report that multiple bacteria, including gram negative organisms (E. coli, E. coli Nissle 1917, Salmonella typhimurium), gram positive organisms (Listeria monocytogenes, Staphylococcus epidermidis), partial anaerobes (Campylobacter jejuni), and obligate anaerobes (Bacteroides thetaiotaomicron, Bifidobacterium breve, Clostridium A4) activate TL1A expression in human APC, including monocytes and monocyte-derived DC. Bacterially induced TL1A mRNA expression correlates with the detection of TL1A protein levels. TL1A induced by bacteria is mediated in part by the TLR signaling pathway and inhibited by downstre...
Source: European Journal of Immunology - October 15, 2009 Category: Allergy & Immunology Authors: David Q. Shih, Lola Y. Kwan, Valerie Chavez, Offer Cohavy, Rivkah Gonsky, Elmer Y. Chang, Christopher Chang, Charles O. Elson, Stephan R. Targan Source Type: journals

Outer membrane vesicle-mediated release of cytolethal distending toxin (CDT) from Campylobacter jejuniEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Our results strongly suggest that the release of outer membrane vesicles is functioning as a route of C. jejuni to deliver all the subunits of CDT toxin (CdtA, CdtB, and CdtC) to the surrounding environment, including infected host tissue. (Source: BioMed Central)
Source: BioMed Central - October 15, 2009 Category: Journals (General) Authors: Barbro LindmarkPramod RompikuntalKarolis VaitkeviciusTianyan SongYoshimitsu MizunoeBernt Eric UhlinPatricia GuerrySun Nyunt Wai Source Type: journals

Polyphosphate Kinase 1 is Important for VBNC Formation, Natural Transformation and Antimicrobial Resistance in Campylobacter jejuni.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Campylobacter jejuni, a Gram-negative, microaerophilic bacterium, is a predominant cause of bacterial gastroenteritis in humans. Although considered fragile, fastidious and lacking many classical stress response mechanisms, C. jejuni exhibits a remarkable capacity for survival and adaptation, successfully infecting humans and persisting in the environment. Consequently, understanding the physiological and genetic properties that allow C. jejuni to survive and adapt to various stress conditions is crucial for therapeutic interventions. Of importance is polyphosphate kinase 1 (PPK1), which is a key enzyme mediating the s...
Source: Applied and Environmental Microbiology - October 15, 2009 Category: Microbiology Authors: Gangaiah D, Kassem II, Liu Z, Rajashekara G Tags: Appl Environ Microbiol Source Type: journals

Microbial induction of inflammatory bowel disease associated gene TL1A (TNFSF15) in antigen presenting cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we report that multiple bacteria, including gram negative organisms (E. coli, E. coli Nissle 1917, Salmonella typhimurium), gram positive organisms (Listeria monocytogenes, Staphylococcus epidermidis), partial anaerobes (Campylobacter jejuni), and obligate anaerobes (Bacteroides thetaiotaomicron, Bifidobacterium breve, Clostridium A4) activate TL1A expression in human APC, including monocytes and monocyte-derived DC. Bacterially induced TL1A mRNA expression correlates with the detection of TL1A protein levels. TL1A induced by bacteria is mediated in part by the TLR signaling pathway and inhibited by downstre...
Source: European Journal of Immunology - October 15, 2009 Category: Allergy & Immunology Authors: Shih DQ, Kwan LY, Chavez V, Cohavy O, Gonsky R, Chang EY, Chang C, Elson CO, Targan SR Tags: Eur J Immunol Source Type: journals

Molybdenum and tungsten in Campylobacter jejuni: their physiological role and identification of separate transporters regulated by a single ModE-like proteinEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Campylobacter jejuni is an important human pathogen that causes millions of cases of food-borne enteritis each year. The C. jejuni respiratory chain is highly branched and contains at least four enzymes predicted to contain a metal binding pterin (MPT), with the metal being either molybdenum or tungsten. Also predicted are two separate transport systems, one for molybdenum encoded by modABC and a second for tungsten encoded by tupABC. Both transport systems were mutated and the activities of the four predicted MPT-containing enzymes were assayed in the presence of molybdenum and tungsten in wild-type and mod and tup backgr...
Source: Molecular Microbiology - October 14, 2009 Category: Microbiology Authors: Michael E. Taveirne, Michael L. Sikes, Jonathan W. Olson Source Type: journals

Development and Evaluation of Internal Amplification Controls (IACs) for use in a real time duplex PCR assay for detection of Campylobacter coli and C. jejuni.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A common problem of both conventional and real time PCR assays is failure of DNA amplification due to the presence of inhibitory substances in samples. In view of this, our aim was to develop and evaluate Internal Amplification Controls (IACs) for use with an existing duplex real time PCR assay for Campylobacter coli and C. jejuni. Both competitive and non-competitive IACs were developed and evaluated. The competitive approach involved a DNA fragment of the coding region of the viral fish haemorrhagic septicaemia virus, flanked by the mapA PCR primers, whilst the non-competitive approach utilised an extra set of univer...
Source: Journal of Medical Microbiology - October 14, 2009 Category: Microbiology Authors: Randall L, Leema F, Rodgers J, Vidal A, Clifton-Hadley F Tags: J Med Microbiol Source Type: journals

A role for tungsten in the biology of Campylobacter jejuni: tungstate stimulates formate dehydrogenase activity and is transported via an ultra-high affinity ABC system distinct from the molybdate transporterEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The food-borne pathogen Campylobacter jejuni possesses no known tungstoenzymes, yet encodes two ABC transporters (Cj0300[ndash]0303 and Cj1538[ndash]1540) homologous to bacterial molybdate (ModABC) uptake systems and the tungstate transporter (TupABC) of Eubacterium acidaminophilum respectively. The actual substrates and physiological role of these transporters were investigated. Tryptophan fluorescence spectroscopy and isothermal titration calorimetry of the purified periplasmic binding proteins of each system revealed that while Cj0303 is unable to discriminate between molybdate and tungstate (KD values for both ligands ...
Source: Molecular Microbiology - October 12, 2009 Category: Microbiology Authors: Jonathan P. Smart, Matthew J. Cliff, David J. Kelly Source Type: journals

Study of Cytolethal Distending Toxin (cdt) in Campylobacter coli Using a Multiplex Polymerase Chain Reaction Assay and its Distribution Among Clinical and Food StrainsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Foodborne Pathogens and Disease , Vol. 0, No. 0. (Source: Foodborne Pathogens and Disease)
Source: Foodborne Pathogens and Disease - October 12, 2009 Category: Food Science Tags: article Source Type: journals

AI-2 does not Function as a Quorum Sensing Molecule in Campylobacter jejuni during exponential growth in vitroEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Taken together these results led us to conclude that there is no evidence for the role of AI-2 in cell-to-cell communication in C. jejuni under the growth conditions used, and that the effects of the luxS mutation on the transcriptome are related to the consequential loss of function in the activated methyl cycle. (Source: BMC Microbiology - Latest articles)
Source: BMC Microbiology - Latest articles - October 7, 2009 Category: Microbiology Authors: Kathryn HolmesTimothy TavenderKlaus WinzerJerry WellsKim Hardie Source Type: journals

Food poisoning bug in chickenEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A government food survey released yesterday has found that most raw chicken sold in the UK contains campylobacter, a bacterium that can cause food poisoning. The survey, by the Food Standards Agency (FSA), checked for the presence of campylobacter in over 3,000 samples of fresh chicken, and found that 65% were contaminated with traces of the bacteria. Campylobacter is estimated to cause 300,000 cases of food poisoning annually. While a number of newspaper reports today described it as a “deadly bug”, only around 70 of these cases are fatal each year . However, the risk of food poisoning can be avoided by cooking meat ...
Source: NHS News Feed - October 7, 2009 Category: Consumer Health News Tags: QA articles Food/diet Source Type: news

Three-quarters of fresh chicken bought on High Street 'contains bug that kills 80 Britons a year'Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Three-quarters of fresh British chicken is contaminated with the potentially lethal food-poisoning bug campylobacter. (Source: the Mail online | Health)
Source: the Mail online | Health - October 7, 2009 Category: Consumer Health News Source Type: news

Effects of Esculentoside A on Autoimmune Syndrome Induced by Campylobacter Jejuni in Mice and its Modulation on T-lymphocyte Proliferation and Apoptosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Esculentoside A (EsA), a saponin isolated from the root of Phytolacca esculenta, has been reported to exert anti-inflammatory effects in several animal models of acute and chronic inflammation by inhibiting the production and activity of pro-inflammatory cytokines in macrophages and epithelial cells. However, little is known about its modulation on T cells. In the present study, we further investigated its potential in treatment of autoimmune disease and its modulation on T cells, using an experimental autoimmune model established through immunizing mice with campylobacter jejuni strain CJ-S(131) in Freund s complete a...
Source: International Immunopharmacology - October 5, 2009 Category: Allergy & Immunology Authors: Hu Z, Qiu L, Xiao Z, Wang J, Yu Q, Li J, Feng H, Guo C, Zhang J Tags: Int Immunopharmacol Source Type: journals

Reductions of escherichia coli, coliforms, aerobic plate counts and campylobacter jejuni by a small-scale, high-pressure system devised to clean a miniaturized poultry giblets transport systemEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The efficacy of using direct high-pressure hot water (60C, 140F) and a quaternary ammonium compound to clean the inside of stainless steel pipe used to transport chicken giblets was evaluated. The giblets were collected from a commercial processing plant and were inoculated with Campylobacter jejuni. The cleaning system was effective in reducing the numbers of inoculated C. jejuni and naturally occurring mesotrophic bacteria (aerobic plate counts) on the inside surface of the stainless steel pipe used to transport the giblets. However, the decreases in naturally occurring Escherichia coli and coliforms were not significant...
Source: Journal of Food Safety - October 4, 2009 Category: Food Science Authors: OMAR A. OYARZABAL, JEFFREY W. FERGUS, ROBERT S. MILLER, WILLIAM F. GALE, DONALD E. CONNER Source Type: journals

Comparative assessment of standard culture and real-time polymerase chain reaction to detect campylobacter jejuni in retail chicken samplesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Contamination of poultry by Campylobacter is a significant source of human diarrheal illness. The purpose of this study was to compare standard culture-based methods and real-time polymerase chain reaction (RT-PCR) for detection of Campylobacter jejuni from retail chicken samples. Culture methods were compared with RT-PCR (without enrichment) for detection of C. jejuni in naturally contaminated chicken samples. Purchased chicken samples (n = 43) were collected from four supermarkets. C. jejuni was detected by direct plating to selective agar (DPSA; 5/43, 11.6%), RT-PCR (15/43, 34.9%) and Bolton's enrichment (BE; 8/43, 41.9...
Source: Journal of Food Safety - October 4, 2009 Category: Food Science Authors: ARADOM DEBRETSION, TSEGAYE HABTEMARIAM, SAUL WILSON, BERHANU TAMERU, IRENE V. WESLEY, TESHOME YEHUALAESHET Source Type: journals

PATHOGEN DETECTION IN FOOD MICROBIOLOGY LABORATORIES: AN ANALYSIS OF QUALITATIVE PROFICIENCY TEST DATA, 1999–2007Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this study was to assess laboratories' ability to detect or rule out the presence of four common food pathogens: Escherichia coli O157:H7, Salmonella spp., Listeria monocytogenes and Campylobacter spp. To do this, qualitative proficiency test data provided by one proficiency test provider from 1999 to 2007 were examined. The annual and cumulative 9-year percentages of false-negative and false-positive responses were calculated. The cumulative 9-year false-negative rates were 7.8% for E. coli O157:H7, 5.9% for Salmonella spp., 7.2% for L. monocytogenes and 13.6% for Campylobacter spp. Atypical strains and l...
Source: Journal of Food Safety - October 4, 2009 Category: Food Science Authors: DANIEL C. EDSON, SUE EMPSON, LAURA D. MASSEY Source Type: journals

Reclassification of Bacteroides ureolyticus as Campylobacter ureolyticus comb. nov.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The protein profiles, genomic amplified fragment length polymorphism patterns, 16S rRNA and cpn60 gene sequences of a diverse collection of 26 Bacteroides ureolyticus strains, along with published data on its DNA base, respiratory quinone and cellular fatty acid compositions, were used to reassess the taxonomy of this bacterium. The results demonstrate that this organism is most appropriately allocated into the genus Campylobacter. The presence of much higher amounts of 18:1omega7c in its cellular fatty acid profile and its ability to digest gelatine and casein are the characteristics that differentiate it from present...
Source: International Journal of Systematic and Evolutionary Microbiology - October 1, 2009 Category: Microbiology Authors: Vandamme P, Debruyne L, De Brandt E, Falsen E Tags: Int J Syst Evol Microbiol Source Type: journals

Long term survival of Campylobacter jejuni at low temperature is dependent on polynucleotide phosphorylase activity.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we demonstrate the role of PNPase at low temperature survival of C. jejuni and therefore attribute a novel biological function to PNPase directly related to human health. PMID: 19801468 [PubMed - as supplied by publisher] (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - October 1, 2009 Category: Microbiology Authors: Haddad N, Burns CM, Bolla JM, Prévost H, Fédérighi M, Drider D, Cappelier JM Tags: Appl Environ Microbiol Source Type: journals

Mechanically ventilated broiler sheds - a source of aerosolized Salmonella, Campylobacter, and Escherichia coli?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study assessed the levels of two key pathogens, Salmonella and Campylobacter, along with the indicator organism E. coli in aerosols within and outside poultry sheds. The study ranged over a three year period on four poultry farms and consisted of 6 trials across the boiler production cycle of around 55 days. Weekly testing of litter and aerosols was carried out through the cycle. A key point that emerged is that the levels of airborne bacteria are linked to the levels of these bacteria in litter. This hypothesis was demonstrated by E. coli. The typical levels of E. coli in litter were around 10(8)cfu g(-1) and as a co...
Source: Applied and Environmental Microbiology - October 1, 2009 Category: Microbiology Authors: Chinivasagam HN, Tran T, Maddock L, Gale A, Blackall PJ Tags: Appl Environ Microbiol Source Type: journals

Pathogenicity of an emergent, ovine abortifacient Campylobacter jejuni clone orally inoculated into pregnant guinea pigs.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions and Clinical Relevance-This study revealed that C jejuni IA 3902 was a unique, highly abortifacient strain with the ability to colonize the intestines, induce systemic infection, and cause abortion because of its affinity for the fetoplacental unit. Guinea pigs could be effectively used in the study of septic abortion after oral inoculation with this Campylobacter strain. PMID: 19795942 [PubMed - in process] (Source: American Journal of Veterinary Research)
Source: American Journal of Veterinary Research - September 30, 2009 Category: Veterinary Research Authors: Burrough ER, Sahin O, Plummer PJ, Zhang Q, Yaeger MJ Tags: Am J Vet Res Source Type: journals

A modified Weibull model for describing the survival of Campylobacter jejuni in minced chicken meat.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Campylobacter is one of the leading causes of foodborne bacterial enteritis. Since chicken meat may be an important source of C. jejuni, the aims of this study were (i) to evaluate the survival/inactivation of C. jejuni strain 49/7R and its antimicrobial resistant variants (49/7RAT and 49/7RATCIP32) in minced chicken meat during extended storage at temperatures ranging from -20 degrees C to 25 degrees C and (ii) to test the suitability of the Weibull model for predicting the inactivation of C. jejuni in minced chicken meat in a wide range of temperatures. Minced chicken meat samples were inoculated with C. jejuni and l...
Source: International Journal of Food Microbiology - September 29, 2009 Category: Food Science Authors: González M, Skandamis PN, Hänninen ML Tags: Int J Food Microbiol Source Type: journals

Contribution of the Multidrug Efflux Transporter CmeABC to Antibiotic Resistance in Different Campylobacter SpeciesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Foodborne Pathogens and Disease , Vol. 0, No. 0. (Source: Foodborne Pathogens and Disease)
Source: Foodborne Pathogens and Disease - September 28, 2009 Category: Food Science Tags: article Source Type: journals

Extremely low-birthweight neonate with prenatal Campylobacter infectionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Pediatrics International)
Source: Pediatrics International - September 27, 2009 Category: Pediatrics Authors: Tatsuo Nagashima, Masahisa Kobayashi, Satoshi Teramoto, Erika Okano, Takayuki Yokoi, Yoshikatsu Eto Tags: PATIENT REPORTS Source Type: journals

Distribution of periodontopathic bacterial species in Japanese children with developmental disabilitiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We found that approximately one-fourth of the present subjects with disabilities who possessed at least one of T. denticola, T. forsythia, and C. rectus were at possible risk for periodontitis. Follow-up examinations as well as preventive approaches should be utilized for such individuals. (Source: BMC Oral Health - Latest articles)
Source: BMC Oral Health - Latest articles - September 22, 2009 Category: Dentistry Authors: Shuhei NakaAki YamanaKazuhiko NakanoRena OkawaKazuyo FujitaAyuchi KojimaHirotoshi NemotoRyota NomuraMichiyo MatsumotoTakashi Ooshima Source Type: journals

Survival mechanisms and culturability of Campylobacter jejuni under stress conditions.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Culture-based isolation and enumeration of bacterial human pathogens from environmental and human food samples has significant limitations. Many pathogens enter a viable but non-culturable (VBNC) state in response to stress, and cannot be detected via culturing methods. Favourable growth conditions with a source of energy and an ideal stoichiometric ratio of carbon to inorganic elements can reverse this VBNC state. This review will focus on the bacterium Campylobacter jejuni which is a leading cause of food borne illness in the developed world. C. jejuni can enter a VBNC state in response to extremes in: pH, moisture c...
Source: Antonie van Leeuwenhoek - September 22, 2009 Category: Microbiology Authors: Jackson DN, Davis B, Tirado SM, Duggal M, van Frankenhuyzen JK, Deaville D, Wijesinghe MA, Tessaro M, Trevors JT Tags: Antonie Van Leeuwenhoek Source Type: journals