Cell Biochemistry and FunctionThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

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The novel ruthenium - [gamma]-linolenic complex [Ru2(aGLA)4Cl] inhibits C6 rat glioma cell proliferation and induces changes in mitochondrial membrane potential, increased reactive oxygen species generation and apoptosis in vitroEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study reports the synthesis of a novel compound with the formula [Ru2(aGLA)4Cl] according to elemental analyses data, referred to as Ru2GLA. The electronic spectra of Ru2GLA is typical of a mixed valent diruthenium(II,III) carboxylate. Ru2GLA was synthesized with the aim of combining and possibly improving the anti-tumour properties of the two active components ruthenium and [gamma]-linolenic acid (GLA). The properties of Ru2GLA were tested in C6 rat glioma cells by analysing cell number, viability, lipid droplet formation, apoptosis, cell cycle distribution, mitochondrial membrane potential and reactive oxygen...
Source: Cell Biochemistry and Function - November 19, 2009 Category: Biochemistry Authors: Geise Ribeiro, Marcel Benadiba, Denise de Oliveira Silva, Alison Colquhoun Source Type: journals

Age-related effects of DHEA on peripheral markers of oxidative stressEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ageing is an inevitable biological process characterized by a general decline in various physiological functions. DHEA and DHEAS levels are maximal between the second and third life decades, then start to decline 2% per year, leaving a residual of 10-20% of the peak production by the eighth decade. Erythrocytes are exposed to frequent oxidative stress due to the oxygen radicals continuously generated by haemoglobin auto-oxidation. We investigated DHEA chronic (10 mg/kg, subcutaneously, for 5 weeks) effects over oxidative stress markers in erythrocytes of male Wistar rats of 3, 13 and 18 month-old. In the 13 month-old group...
Source: Cell Biochemistry and Function - November 18, 2009 Category: Biochemistry Authors: Maria Helena Vianna Metello Jacob, Daiane da R. Janner, Matheus Parmegiani Jahn, Luiz Carlos Kucharski, Adriane Belló-Klein, Maria Flavia Marques Ribeiro Source Type: journals

p63 and its isoforms as markers of rat oral mucosa epidermal stem cells in vitroEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Although techniques for purifying epidermal stem cells (ESCs) have been established, enriching a pure population of viable ESCs is still a challenging task. One approach toward enhancing the purity and viability of ESCs involves cell markers. While evidence suggests that p63 plays a role in maintaining the population of ESCs, whether p63 can function as a specific marker for ESCs is unclear. We isolated and cultured oral ESCs and illustrated the expression of p63 and its isoforms in rat oral mucosa tissues and stem cells before and after differentiation. Semi-reverse transcription PCR detected the TA, [Delta]N, [alpha] and...
Source: Cell Biochemistry and Function - November 16, 2009 Category: Biochemistry Authors: Qian Tao, Bin Qiao, Biao Lv, Chaoqun Zheng, Zhifeng Chen, Hongzhang Huang Source Type: journals

Differential responses to oxidative stress and calcium influx on expression of the transforming growth factor-[beta] family in myoblasts and myotubesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Changes in gene expression of TGF-[beta] family members and their receptors in response to treatment with H2O2 and a calcium ionophore, A23187, were examined in C2C12 myoblasts and myotubes. The expression of Myf5, an initial regulator of myogenesis, was increased by A23187, and H2O2 inhibited the up-regulation of Myf5. Treatment with H2O2 decreased the expression of MHC IIb, a protein component of the myofibrils, irrespective of the presence of A23187, suggesting an inhibitory role of oxidative stress for myogenesis. Expression of ligands and receptors for the TGF-[beta] family was modulated in response to H2O2 and A23187...
Source: Cell Biochemistry and Function - November 16, 2009 Category: Biochemistry Authors: Yuuma Furutani, Masaru Murakami, Masayuki Funaba Source Type: journals

Apoptosis-inducing effects of Morinda citrifolia L. and doxorubicin on the Ehrlich ascites tumor in Balb-c miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Morinda citrifolia L. (Noni) is a herbal remedy with promising anti-cancer properties. However, its effects on various cancers are to be investigated to make a firm conclusion before implementing it into the clinical practice. Therefore, we investigated the cytotoxic potential of noni on Ehrlich ascites tumor grown in female Balb-c mice and also combined it with a potent anti-cancer agent, doxorubicin. One group received noni only (n = 8), another one doxorubicin (n = 8), and the other one noni + doxorubicin (n = 8) for 14 days after the inoculation of cells. The control group (n = 7) received 0.9% NaCl only. We found that...
Source: Cell Biochemistry and Function - November 11, 2009 Category: Biochemistry Authors: Elif [Idot]lkay Ta[scedil]k[inodot]n, Kadriye Akgün-Dar, Ay[scedil]egül Kapucu, Esma Osanç, Hüsniye Do[gbreve]ruman, Hakan Eraltan, Engin Ulukaya Source Type: journals

Analysis of paraoxonase 1 (PON1) genetic polymorphisms and activities as risk factors for ischemic stroke in Turkish populationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we aimed to determine the importance of -107T/C, 192Q/R and 55L/M polymorphisms of PON1 gene and three PON1 activities (diazoxonase, paraoxonase, arylesterase) as risk factors for ischemic stroke.Study population was comprised of 172 unrelated adult Caucasian patients with acute hemispheric ischemic stroke and 105 symptom-free controls. Genotypes were attained by PCR followed by restriction enzyme digestion and phenotypes were determined by spectrophotometric assays.This is the first study analyzing diazoxonase activity as a risk factor for ischemic stroke. Nevertheless, diazoxonase, paraoxonase and aryleste...
Source: Cell Biochemistry and Function - November 10, 2009 Category: Biochemistry Authors: Birsen Can Demirdö[gbreve]en, [Scedil]eref Demirkaya, Aysun Türkano[gbreve]lu, Semai Bek, Emel Ar[inodot]nç, Orhan Adal[inodot] Source Type: journals

Effects of supplementation with free glutamine and the dipeptide alanyl-glutamine on parameters of muscle damage and inflammation in rats submitted to prolonged exerciseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we investigated the effect of the supplementation with the dipeptide L-alanyl-L-glutamine (DIP) and a solution containing L-glutamine and L-alanine on plasma levels markers of muscle damage and levels of pro-inflammatory cytokines and glutamine metabolism in rats submitted to prolonged exercise. Rats were submitted to sessions of swim training for 6 weeks. Twenty-one days prior to euthanasia, the animals were supplemented with DIP (n = 8) (1.5 g.kg-1), a solution of free L-glutamine (1 g.kg-1) and free L-alanine (0.61 g.kg-1) (G&A, n = 8) or water (control (CON), n = 8). Animals were killed at rest before (R...
Source: Cell Biochemistry and Function - November 3, 2009 Category: Biochemistry Authors: Vinicius Fernandes Cruzat, Marcelo Macedo Rogero, Julio Tirapegui Source Type: journals

Transcriptional regulation of IER3IP1 gene by tumor necrosis factor-[alpha] and Sp family proteinsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this report, we investigated the molecular mechanism by which the expression of IER3IP1 gene is regulated by cloning the 5[prime] flanking region of the human IER3IP1 gene for various promoter studies. Deletion analysis was used to identify the basal promoter activity retained at -298/-59 region and mutation analysis proved that Sp1 is a transcriptional activator of this gene expression. As an early response gene, IER3IP1 showed an increase in transcription in response to tumor necrosis factor [alpha] (TNF-[alpha]) in a time- and dose-dependent manner. This inducible response to TNF-[alpha] is mediated by the demonstrat...
Source: Cell Biochemistry and Function - November 2, 2009 Category: Biochemistry Authors: Wai Han Yiu, Tsz Lun Yeung, Jodie Wing Ming Poon, Stephen Kwok Wing Tsui, Kwok Pui Fung, Mary Miu Yee Waye Source Type: journals

Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid ratsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The purpose of this study was to determine whether decreased oxidative stress would increase the resistance to cardiac contracture induced by H2O2 in hypothyroid rats. Male Wistar rats were divided into two groups: control and hypothyroid. Hypothyroidism was induced via thyroidectomy. Four weeks post surgery, blood samples were collected to perform thyroid hormone assessments, and excised hearts were perfused at a constant flow with or without H2O2 (1 mmol/L), being divided into two sub-groups: control, hypothyroid, control + H2O2, hypothyroid + H2O2. Lipid peroxidation (LPO) was evaluated by chemiluminescence (CL) and thi...
Source: Cell Biochemistry and Function - November 2, 2009 Category: Biochemistry Authors: Alex Sander da Rosa Araujo, Madalena Freitas Silva de Miranda, Ubirajara Oliveira de Oliveira, Tânia Fernandes, Susana Llesuy, Luiz Carlos Rios Kucharski, Neelam Khaper, Adriane Belló-Klein Source Type: journals

Development and optimization of a cell-based assay for the selection of synthetic compounds that potentiate bone morphogenetic protein-2 activityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we describe the development and optimization of a cell-based assay that is sensitive, reproducible, and reliable in identifying reagents that potentiate the effects of BMP-2 in inducing transdifferentiation of C2C12 myoblasts into the osteoblastic phenotype. The assay is based on a BMP-responsive Smad1-driven luciferase reporter gene. LIM mineralization protein-1 (LMP-1) is a novel intracellular LIM domain protein that has been shown by our group to enhance cellular responsiveness to BMP-2. Our previous report elucidated that the binding of LMP-1 with the WW2 domain in Smad ubiquitin regulatory factor-1 (Smu...
Source: Cell Biochemistry and Function - October 27, 2009 Category: Biochemistry Authors: Motohiro Okada, Sreedhara Sangadala, Yunshan Liu, Munehito Yoshida, Boojala Vijay B. Reddy, Louisa Titus, Scott D. Boden Source Type: journals

Ceramide induces endothelial cell senescenceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ceramide has been proposed to be a mediator of replicative senescence. Our aim was to determine whether ceramide induces senescence in vascular endothelial cells. Human umbilical vein endothelial cells were cultured to different population doubling levels and ceramide levels were quantitated. The endogenous levels of ceramide increased 2.4-fold with senescence onset. Low passage cells were chronically treated with exogenous C6-ceramide. This treatment induced a senescent phenotype as measured by an inhibition of cell proliferation and DNA replication while increasing senescence-associated [beta]-galactosidase expression. T...
Source: Cell Biochemistry and Function - October 19, 2009 Category: Biochemistry Authors: Mark E. Venable, Xinhe Yin Source Type: journals

Integrating the cell stress response: a new view of molecular chaperones as immunological and physiological homeostatic regulatorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The response of cells to stress was first documented in the 1960s and 1970s and the molecular nature of the families of proteins that subserve this vital response, the molecular chaperones, were identified and subjected to critical study in the period from the late 1980s. This resulted in the rapidly advancing new field of protein folding and its role in cellular function. Emerging at the same time, but initially largely ignored, were reports that molecular chaperones could be released by cells and exist on the outer plasma membrane or in the body fluids. These secreted molecular chaperones were found to have intercellular...
Source: Cell Biochemistry and Function - October 14, 2009 Category: Biochemistry Authors: Brian Henderson Source Type: journals

Changes in the mRNA expression of osteoblast-related genes in response to [beta]3-adrenergic agonist in UMR106 cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, [beta]3-AR activation in rat osteoblasts induced alteration in the expression of osteoblast-related transcription factor genes as well as genes required for bone formation and resorption. The present results also suggest that, besides [beta]2-AR, [beta]3-AR is another AR subtype responsible for the sympathetic nervous system-induced bone remodeling. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - October 13, 2009 Category: Biochemistry Authors: Amporn Nuntapornsak, Kannikar Wongdee, Jirawan Thongbunchoo, Nateetip Krishnamra, Narattaphol Charoenphandhu Source Type: journals

Organ preservation solutions attenuate accumulation and nuclear translocation of hypoxia-inducible factor-1[alpha] in the hepatoma cell line HepG2Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, preservation solutions attenuate accumulation and nuclear translocation of the transcription factor HIF-1[alpha], and this property is seemingly related to their chemical composition (L-arginine, [alpha]-ketoglutarate). Thus, it appears feasible to design preservation solution specifically to modify HIF-1[alpha] accumulation and nuclear translocation. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - October 8, 2009 Category: Biochemistry Authors: Renate Paddenberg, Nele Howold, Christiane Hoger, Hermann Janßen, Veronika Grau, Wolfgang Kummer Source Type: journals

The effect of 5 days of aspartate and asparagine supplementation on glucose transport activity in rat muscleEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The consumption of protein supplements containing amino acids is increasing around the world. Aspartate (Asp) and asparagine (Asn) are amino acids metabolized by skeletal muscle. This metabolism involves biochemical pathways that are involved in increasing Krebs cycle activity via anaplerotic reactions, resulting in higher glutamine concentrations. A connection between amino acid supplementation, glycogen concentration, and glucose uptake has been previously demonstrated. The purpose of this study was to evaluate the effect of Asp and Asn supplementation on glucose uptake in rats using three different glycogen concentratio...
Source: Cell Biochemistry and Function - October 8, 2009 Category: Biochemistry Authors: Antonio H. Lancha Jr, Jacques R. Poortmans, Luciana O. Pereira Source Type: journals

Rate of T alleles and TT genotype at MTHFR 677C->T locus or C alleles and CC genotype at MTHFR 1298A->C locus among healthy subjects in Turkey: impact on homocysteine and folic acid status and reference intervalsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to determine the rate of MTHFR 677C->T and 1298A->C mutations, and their influence on serum folate, Hcy and vitamin B12 status and the reference intervals in 402 healthy Turkish adults. The rate of MTHFR 677C->T or 1298A->C mutations was 50.7% or 54.7%, respectively. The MTHFR 677C->T mutation-specific reference intervals for serum folate and tHcy were characterized by marked shifts in their upper limits. In homozygote subjects for MTHFR 677C->T serum folate concentration was lower and serum tHcy concentration was higher than those in the wild genotype; all subjects had lower serum folate and 54% of...
Source: Cell Biochemistry and Function - September 17, 2009 Category: Biochemistry Authors: Yesim Ozarda, Derya Kaynak Sucu, Banu Hizli, Diler Aslan Source Type: journals

TNF-[alpha], a potent lipid metabolism regulatorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
As a multifunctional cytokine, tumor necrosis factor alpha (TNF-[alpha]) exerts a series of biological actions in different cells, tissues, organs, and species and has been demonstrated to regulate and interfere with energy metabolism, especially lipid homeostasis. A large body of researches suggested that the effects of TNF-[alpha] on lipid metabolism mainly include five aspects: (1) suppresses free fatty acid (FFA) uptake and promotes lipogenesis; (2) induces lipolysis; (3) inhibits lipid-metabolism-related enzymes activity; (4) regulates cholesterol metabolism; (5) regulates other adipocyte-derived adipokines. The molec...
Source: Cell Biochemistry and Function - September 14, 2009 Category: Biochemistry Authors: Xiuping Chen, Keli Xun, Lidian Chen, Yitao Wang Source Type: journals

Effect of stress and stress hormones on the hormone (insulin) binding of TetrahymenaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The unicellular Tetrahymena pyriformis GL produce, store and secrete vertebrate-like hormones. In earlier experiments the effect of different stressors on the hormone levels of Tetrahymena was studied and an elevation of these was found. In the present experiments the hormone binding was investigated, using flow cytometric method. FITC-insulin binding was elevated after concentrated (5, 10, or 20 mg ml-1) NaCl or 0.01%, 0.1%, or 0.05% formaldehyde treatment, or after thermal stress (37°C). Serotonin given together with NaCl increased and together with formaldehyde decreased the binding. Histamine always decreased the bind...
Source: Cell Biochemistry and Function - September 8, 2009 Category: Biochemistry Authors: György Csaba, Éva Pállinger Source Type: journals

Mechanism of cell death by 5-aminolevulinic acid-based photodynamic action and its enhancement by ferrochelatase inhibitors in human histiocytic lymphoma cell line U937Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Photodynamic therapy (PDT) for tumors is based on the tumor-selective accumulation of a photosensitizer, protoporphyrin IX (PpIX), followed by irradiation with visible light. However, the molecular mechanism of cell death caused by PDT has not been fully elucidated. The 5-aminolevulinic acid (ALA)-based photodynamic action (PDA) was dependent on the accumulation of PpIX, the level of which decreased rapidly by eliminating ALA from the incubation medium in human histiocytic lymphoma U937 cells. PDA induced apoptosis characterized by lipid peroxidation, increase in Bak and Bax/Bcl-xL, decrease in Bid, membrane depolarization...
Source: Cell Biochemistry and Function - September 3, 2009 Category: Biochemistry Authors: Takashi Amo, Noriaki Kawanishi, Masataka Uchida, Hirofumi Fujita, Eri Oyanagi, Toshihiko Utsumi, Tetsuya Ogino, Keiji Inoue, Taro Shuin, Kozo Utsumi, Junzo Sasaki Source Type: journals

Effects of insulin replacement on cardiac apoptotic and survival pathways in streptozotocin-induced diabetic ratsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Increased myocyte apoptosis in diabetic hearts has been previously reported. Therefore, the purpose of this study was to evaluate the effects of insulin on cardiac apoptotic, hypertrophic, and survival pathways in streptozotocin (STZ)-induced diabetic rats. Forty-eight male Wistar rats at 8 weeks of age were randomly divided into control group (Control), STZ-induced (65 mg/kg STZ i.v.) Type 1-like diabetic rats (DM), and DM rats with 4 IU insulin replacement (DI) for 4 and 8 weeks, respectively. The levels of protein involved in cardiac apoptotic, hypertrophic, and survival pathways were measured by Western blotting. Cardi...
Source: Cell Biochemistry and Function - August 27, 2009 Category: Biochemistry Authors: Wei-Wen Kuo, Li-Ching Chung, Cheng-Tzu Liu, Shih-Ping Wu, Chia-Hua Kuo, Fuu-Jen Tsai, Chang-Hai Tsai, Min-Chi Lu, Chih-Yang Huang, Shin-Da Lee Source Type: journals

IgLON cell adhesion molecules regulate synaptogenesis in hippocampal neuronsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
IgLON cell adhesion molecules (CAMs) belonging to the immunoglobulin superfamily comprise of LAMP, neurotrimin (Ntm), OBCAM, and Kilon. In the present study, we performed the single and double transfection of IgLON gene constructs into hippocampal neurons in vitro and evaluated synaptic number. The quantitative analysis showed that the single over-expression of LAMP or OBCAM increased synaptic number, while the over-expression of Kilon reduced synaptic number and Ntm had no effects. The double over-expression of Kilon-Ntm, Kilon-OBCAM, LAMP-Ntm, and Ntm-OBCAM decreased synaptic number and that of Kilon-LAMP and LAMP-OBCAM ...
Source: Cell Biochemistry and Function - August 26, 2009 Category: Biochemistry Authors: Takashi Hashimoto, Shohei Maekawa, Seiji Miyata Source Type: journals

Interaction of diphtheria toxin (fragment A) with actinEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It was shown by gel filtration and viscosity measurements that N-terminal fragment (FA) of diphtheria toxin (DT) can interact with both G- and F-actin (filamentous actin). Elution profiles on Sephadex G-100 indicated the formation of a binary complex of fragment A (FA) with globular actin monomer (G-actin), which was inhibited by gelsolin. Deoxyribonuclease I (DNase I) in turn appeared to interact with this complex. Tritiated FA was found to bind to F-actin stoichiometrically. This binding was inhibited again by gelsolin and G-actin, but not by DNase I. The binding of FA inhibited polymerization of G-actin and induced a ti...
Source: Cell Biochemistry and Function - August 25, 2009 Category: Biochemistry Authors: Muhammet Bekta[scedil], Ba[scedil]ak Varol, Rüstem Nurten, Engin Bermek Source Type: journals

A JNK inhibitor SP600125 induces defective cytokinesis and enlargement in P19 embryonal carcinoma cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
While analyzing the role of c-Jun NH2-terminal kinase (JNK) in neurogenesis in P19 embryonal carcinoma cells, we noticed that treatment with SP600125, a JNK inhibitor, increased the cell size markedly. SP600125-induced enlargement of P19 cells was time- and dose-dependent. The increased cell size in response to SP600125 was also detected in B6mt-1 embryonic stem cells. SP600125 treatment inhibited cell growth and increased DNA contents, indicating the inhibition of cell proliferation resulting from endoreduplication. Concurrently, the gene expression of p21, a regulator of G2/M arrest as well as G1 arrest, was increased in...
Source: Cell Biochemistry and Function - August 25, 2009 Category: Biochemistry Authors: Kohei Nakaya, Ryo Ooishi, Masayuki Funaba, Masaru Murakami Source Type: journals

Polymorphisms of DNA base-excision repair genes APE/Ref-1 and XRCC1 are not associated with the risk for Graves' diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Oxidative stress has been implicated in etiopathogenesis of Graves' disease (GD). Increased lipid peroxidation and oxidative DNA damage have been found in GD patients. Oxidative DNA damage is mainly repaired by the base-excision repair (BER) pathway. Polymorphisms in DNA-repair genes have been associated with the increased risk of various diseases and could also be related to the etiology of GD. Therefore, we conducted a study including 197 patients with GD and age- and sex-matched 303 healthy subjects to examine the role of single-nucleotide polymorphisms of BER genes, APE/Ref-1 (codon 148) and XRCC1 (codons 194 and 399) ...
Source: Cell Biochemistry and Function - August 25, 2009 Category: Biochemistry Authors: Semra Do[gbreve]ru-Abbaso[gbreve]lu, Sevda Tanr[inodot]kulu, Evin Ademo[gbreve]lu, Ye[scedil]im Erbil, Ay[scedil]enur Özderya, Berrin Karada[gbreve], Müjdat Uysal Source Type: journals

Changes of glycogen content in liver, skeletal muscle, and heart from fasted ratsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Glycogen content of white and red skeletal muscles, cardiac muscle, and liver was investigated in conditions where changes in plasma levels of non-esterified fatty acids (NEFA) occur. The experiments were performed in fed and 12 and 48 h-fasted rats. The animals were also submitted to swimming for 10 and 30 min. Glycogen content was also investigated in both pharmacologically induced low plasma NEFA levels fasted rats and pharmacologically induced high plasma NEFA levels fed rats. The participation of Akt and glycogen synthase kinase-3 (GSK-3) in the changes observed was investigated. Plasma levels of NEFA, glucose, and in...
Source: Cell Biochemistry and Function - August 25, 2009 Category: Biochemistry Authors: Eduardo Kokubun, Sandro M. Hirabara, Jarlei Fiamoncini, Rui Curi, Horst Haebisch Source Type: journals

Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissuesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Different fat depots contribute differently to disease and function. These differences may be due to the regional variation in cell types and inherent properties of fat cell progenitors. To address the differences of cell types in the adipose tissue from different depots, the phenotypes of freshly isolated adipose tissue-derived cells (ATDCs) from subcutaneous (SC) and omental (OM) adipose tissues were compared using flow cytometry. Our results showed that CD31-CD34+CD45-CD90-CD105-CD146+ population, containing vascular smooth muscle cells and pericytes, was specifically defined in the SC adipose tissue while no such popul...
Source: Cell Biochemistry and Function - August 18, 2009 Category: Biochemistry Authors: Mito Toyoda, Yoshinori Matsubara, Konghua Lin, Keizou Sugimachi, Masutaka Furue Source Type: journals

Chronic exercise decreases cytokine production in healthy rat skeletal muscleEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, exercise training induced decreased cytokine protein expression in the skeletal muscle. These data show that in healthy rats, 8-week moderate-intensity aerobic training down regulates skeletal muscle production of cytokines involved in the onset, maintenance and regulation of inflammation, and that the response is heterogeneous according to fibre composition. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 13, 2009 Category: Biochemistry Authors: Fábio S. Lira, Cristiane H. Koyama, Alex S. Yamashita, José C. Rosa, Nelo E. Zanchi, Miguel L. Batista Jr., Marília C. Seelaender Source Type: journals

Biological markers in cerebrospinal fluid (CSF) and evaluation of in vitro effect of CSF on PC12 cell line viability in Alzheimer's diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The definite diagnosis of Alzheimer's disease (AD) is based on post mortem pathological examination. To date, there is no laboratory test that can discriminate AD patients from healthy individuals. In the perspective of recent knowledge, there are three cerebrospinal fluid (CSF) markers which have the highest sensitivity and specificity: A[beta]1-40, A[beta]1-42, and p-tau. In the present study, 15 'Probable Alzheimer's Disease' (PAD) patients and 15 control subjects were included. PAD patients were selected from the patients of Dokuz Eylül University Neurology Department Dementia outpatient clinic and control subjects we...
Source: Cell Biochemistry and Function - July 27, 2009 Category: Biochemistry Authors: Erdem Yaka, Mehtap Yuksel Egrilmez, Pembe Keskinoglu, Zahide Cavdar, Sermin Genc, Kursad Genc, Leyla [Idot]yilikci, Görsev G. Yener Source Type: journals

Up-regulation of the tight-junction protein ZO-1 by substance P and IGF-1 in A431 cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The formation of a barrier by tight junctions is important in epithelia of various tissues. Substance P (SP) and insulin-like growth factor (IGF)-1 synergistically promote barrier function in the corneal epithelium. We have now examined the effects of SP and IGF-1 on expression of the tight-junction protein zonula occludens (ZO)-1 in A431 human epidermoid carcinoma cells. Reverse transcription-polymerase chain reaction (RT-PCR) and immunoblot analyses revealed that SP and IGF-1 increased the amounts of ZO-1 mRNA and protein in these cells in a concentration-dependent manner, with neither SP nor IGF-1 alone having such an e...
Source: Cell Biochemistry and Function - July 27, 2009 Category: Biochemistry Authors: Ji-Ae Ko, Shizuka Murata, Teruo Nishida Source Type: journals

Vitamin C and E combination modulates oxidative stress induced by X-ray in blood of smoker and nonsmoker radiology techniciansEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
X-ray radiation is detrimental to human cells and may lead to development of life-threatening diseases. Cigarette smoke contains about 500 chemicals that include organic and oxidant compounds whereas vitamin C and E (VCE) have scavenger effects on the compounds. We investigated effects of VCE administration on X-ray-induced oxidative toxicity in blood of smoker and nonsmoker X-ray technicians.Twenty technicians and 30 healthy age-matched subjects control were used in the study. Ten of the X-ray technicians and 15 of the control were smokers. Blood samples were taken from the control. Oral vitamin C (500 mg) and vitamin E (...
Source: Cell Biochemistry and Function - July 26, 2009 Category: Biochemistry Authors: Mustafa Kayan, Mustafa Naz[inodot]ro[gbreve]lu, Ömer Çelik, Kadir Yalman, Halis Köylü Source Type: journals

Effects of [beta]-carotene on antioxidant status in rats with chronic alcohol consumptionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study examined the effects of [beta]-carotene on antioxidant status in rats with chronic alcohol consumption. At the beginning of experiment (week 0), according to both the plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, rats (n = 24) were divided into 3 groups and fed with a standard diet (group C), a diet containing ethanol (group E), or a diet containing ethanol and [beta]-carotene (group E+B). After 10 weeks, plasma AST and ALT, fat accumulation in the liver, antioxidant enzyme activities in erythrocytes and the liver, malondialdehyde (MDA), and [alpha]-tocopherol and retinol...
Source: Cell Biochemistry and Function - July 26, 2009 Category: Biochemistry Authors: Wan-Teng Lin, Chi-Chang Huang, Tien-Jen Lin, Jiun-Rong Chen, Ming-Jer Shieh, Hsiang-Chi Peng, Suh-Ching Yang, Chih-Yang Huang Source Type: journals

Ketogenesis evaluation in perfused liver of diabetic rats submitted to short-term insulin-induced hypoglycemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ketogenesis, inferred by the production of acetoacetate plus ß-hydroxybutyrate, in isolated perfused livers from 24-h fasted diabetic rats submitted to short-term insulin-induced hypoglycemia (IIH) was investigated. For this purpose, alloxan-diabetic rats that received intraperitoneal regular insulin (IIH group) or saline (COG group) injection were compared. An additional group of diabetic rats which received oral glucose (gavage) (100 mg kg-1) 15 min after insulin administration (IIH + glucose group) was included. The studies were performed 30 min after insulin (1.0 U kg-1) or saline injection. The ketogenesis before oct...
Source: Cell Biochemistry and Function - July 21, 2009 Category: Biochemistry Authors: Helenton Cristhian Barrena, Vilma Aparecida Ferreira Godoi Gazola, Maria Montserrat Diaz Pedrosa Furlan, Rosângela Fernandes Garcia, Helenir Medri de Souza, Roberto Barbosa Bazotte Source Type: journals

HERC1 polymorphisms: population-specific variations in haplotype compositionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human HERC1 is one of six HERC proteins and may play an important role in intracellular membrane trafficking. The human HERC1 gene is suggested to have been affected by local positive selection. To assess the global frequency distributions of coding and non-coding single nucleotide polymorphisms (SNPs) in the HERC1 gene, we developed a new simultaneous genotyping method for four SNPs, and applied this method to investigate 1213 individuals from 12 global populations. The results confirmed remarked differences in the allele and haplotype frequencies between East Asian and non-East Asian populations. One of the three common ...
Source: Cell Biochemistry and Function - July 18, 2009 Category: Biochemistry Authors: Isao Yuasa, Kazuo Umetsu, Hiroaki Nishimukai, Yasuo Fukumori, Shinji Harihara, Naruya Saitou, Feng Jin, Prasanta K. Chattopadhyay, Lotte Henke, Jürgen Henke Source Type: journals

Human insulin A-chain peptide analog(s) with in vitro biological activityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In a previous study, we showed that a synthetic human insulin 1-chain analog, named analog (3) was capable of mimicking in vitro effects of native insulin, including stimulation of cell proliferation, glucose uptake and glycogen synthesis. Here, we have synthesized three new analogs (6, 9, 12) of the human A-chain, bearing or not their N- or C-terminal residue, to determine the structural features which are responsible for their biological properties. In vitro experiments clearly demonstrated that the N-terminal part of the peptides is required for the biological activity of the molecules, suggesting its crucial role in th...
Source: Cell Biochemistry and Function - July 16, 2009 Category: Biochemistry Authors: Guillaume Le Flem, Julien Pecher, Valerie Le Flem-Bonhomme, François-Yves Dupradeau, Jacques Rochette, Jean-Pierre Pujol, Patrick Bogdanowicz Source Type: journals

Pulmonary antioxidant defences and protein damage during the ageing process of both sexesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The free radical theory holds that the senescence is caused by oxidative damage that results from an imbalance between reactive oxygen and nitrogen species (RONS) and antioxidant defences. Hence, it plays an important role in the field of gerontology. We evaluated, in male and female rats, the activities of the antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx), and total superoxide dismutase (tSOD), as well as oxidative protein damage in pulmonary tissue at 3, 6, 12, and 20 months of age. The results show an increase in the activities of all antioxidant enzymes at 12 months of age in female rats, suggesting ...
Source: Cell Biochemistry and Function - July 8, 2009 Category: Biochemistry Authors: Fernanda Schäfer Hackenhaar, Tiago Boeira Salomon, Paulo V. Gil Alabarse, Guilherme Ehrenbrink, Mara Silveira Benfato Source Type: journals

Determination of the lactate threshold and maximal blood lactate steady state intensity in aged ratsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The reliability of the lactate threshold (LT) determined in aged rats and its validity to identify an exercise intensity corresponding to the maximal blood lactate steady state (MLSS) were analyzed. Eighteen male aged Wistar rats ([sim]365 days) were submitted to two incremental swimming tests until exhaustion, consisting of an initial load corresponding to 1% of body mass (BM) and increments of 1% BM at each 3-min with blood lactate ([lac]) measurements. The LT was determined by visual inspection (LTV) as well by applying a polynomial function on the [lac]/workload ratio (LTP) by considering the vertices of the curve. For...
Source: Cell Biochemistry and Function - July 7, 2009 Category: Biochemistry Authors: Rafael Rodrigues Cunha, Verusca Najara de Carvalho Cunha, Paulo Russo Segundo, Sérgio Rodrigues Moreira, Eduardo Kokubun, Carmen Sílvia Grubert Campbell, Ricardo Jacó de Oliveira, Herbert Gustavo Simões Source Type: journals

TpMRK regulates cell division of Tetrahymena in response to oxidative stressEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
TpMRK was identified as a stress-responsive mitogen activated protein kinase (MAPK)-related kinase and has been shown to play a critical role in the stress signalling in Tetrahymena cells. Here, we found that the mRNA expression of TpMRK was correlated with cell division of Tetrahymena with decreased expression occurring in cells prior to entering synchronous cell division induced by heat treatment. Notably, cell division was delayed with a lower division index of 40% after exposure to hydrogen peroxide while 85% of cells underwent cell division synchronously at 75 min after heat treatment without the oxidative exposure. F...
Source: Cell Biochemistry and Function - July 6, 2009 Category: Biochemistry Authors: Wenzhou Li, Siwei Zhang, Osamu Numata, Yoshinori Nozawa, Shulin Wang Source Type: journals

The relationship between cell membrane damage and lipid peroxidation under the condition of hypoxia-reoxygenation: analysis of the mechanism using antioxidants and electron transport inhibitorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We consecutively observed lipid peroxidation and cell membrane damage under the condition of hypoxia-reoxygenation (H/R) in cells and analyzed their mechanisms by using electron transport inhibitors and an antioxidant. In H/R experiments, lipid peroxidation and cell membrane damage were observed during the hypoxia phase. In the reoxygenation phase, lipid peroxidation stopped, while cell membrane damage did not. An antioxidant, n-acetylcystein (NAC), and potassium cyanide (KCN) inhibited lipid peroxidation and cell membrane damage, while rotenone did not inhibit either of them. Although antimycin A did not inhibit lipid per...
Source: Cell Biochemistry and Function - June 29, 2009 Category: Biochemistry Authors: Daisuke Yajima, Hisako Motani, Mutsumi Hayakawa, Yayoi Sato, Kaoru Sato, Hirotaro Iwase Source Type: journals

ICAT as a potential enhancer of monocytic differentiation: implications from the comparative proteome analysis of the HL60 cell line stimulated by all-trans retinoic acid and NSC67657Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A novel sterol mesylate compound (NSC67657) was recently identified and reported by National Cancer Institute that could efficiently induce the differentiation of HL60 cells into monocytes in vitro and in vivo. The expression of many proteins would have been changed during the differentiation process, and some proteins may have played key roles in the differentiation of HL60 cell line induced by this drug. Therefore, we treated HL60 cells with NSC67657 and all-trans retinoic acid (ATRA) to identify the differentially expressed proteins and determine their functions in cellular differentiation. Of the 45 differentially expr...
Source: Cell Biochemistry and Function - June 29, 2009 Category: Biochemistry Authors: Weijia Wang, Xiuming Zhang, Kaiying Deng, Shifeng Huang, Xiaoqin Mao, Yurong Fu, Zhengjun Yi, Yurong Yan, Zongyin Qiu Source Type: journals

Effect of Terminalia chebula aqueous extract on oxidative stress and antioxidant status in the liver and kidney of young and aged ratsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We evaluated the preventive effects of Terminalia chebula (T. chebula) aqueous extract on oxidative and antioxidative status in liver and kidney of aged rats compared to young albino rats. The concentrations of malondialdehyde (MDA), lipofuscin (LF), protein carbonyls (PCO), activities of xantione oxidase (XO), manganese-superoxide dismutase (MnSOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S-transferase (GST), and glucose-6-phosphate dehydrogenase (G6PDH), levels of glutathione (GSH), vitamin C and vitamin E were used as biomarkers. In the liver and kidney of aged animals, enha...
Source: Cell Biochemistry and Function - June 24, 2009 Category: Biochemistry Authors: Ramalingam Mahesh, Shanmugham Bhuvana, Vava Mohaideen Hazeena Begum Source Type: journals

Pulsatilla decoction and its active ingredients inhibit secretion of NO, ET-1, TNF-[alpha], and IL-1[alpha] in LPS-induced rat intestinal microvascular endothelial cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To investigate the pharmacological mechanism of the traditional Chinese medicine, Pulsatilla decoction (PD), the levels of nitric oxide (NO), endothelin-1 (ET-1), tumor necrosis factor-[alpha] (TNF-[alpha]), and interleukin-1[alpha] (IL-1[alpha]) secreted by cultured rat intestinal microvascular endothelial cells (RIMECs) were determined after treatment with PD and its seven active ingredients, namely anemoside B4, anemonin, berberine, jatrorrhizine, palmatine, aesculin, and esculetin. RIMECs were challenged with lipopolysaccharide (LPS) at 1 µg ml-1 for 3 h and then treated with PD at 1, 5, and 10 mg ml-1 and its seven i...
Source: Cell Biochemistry and Function - May 28, 2009 Category: Biochemistry Authors: Yiyi Hu, Xi Chen, Huiqin Duan, Yuanliang Hu, Xiang Mu Source Type: journals

Antioxidant enzymes and melatonin levels in patients with bronchial asthma and chronic obstructive pulmonary disease during stable and exacerbation periodsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, we observed that oxidative stress in the exacerbation period of patients with BA and COPD increased whereas the antioxidant enzymes and melatonin values reduced. The episodes of BA or COPD might be associated with elevated levels of oxidative stress. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - May 27, 2009 Category: Biochemistry Authors: Nurhan Gumral, Mustafa Naz[inodot]roglu, Kurtulus Ongel, Emine Dasdibi Beydilli, Fehmi Ozguner, Recep Sutcu, Sadettin Cal[inodot]skan, Ahmet Akkaya Source Type: journals

Genotyping of five single nucleotide polymorphisms in the OCA2 and HERC2 genes associated with blue-brown eye color in the Japanese populationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human eye color is a polymorphic phenotype influenced by multiple genes. It has recently been reported that three single nucleotide polymorphisms (SNPs) within intron 1 of the OCA2 gene (rs7495174, rs4778241, rs4778138) and two SNPs in intron 86 (rs12913832) and the 3[prime] UTR region (rs1129038) of the HERC2 gene - located in the upstream of the OCA2 locus - have a high statistical association with human eye color. The present study is the first to examine in detail the genotype and haplotype frequencies for these five SNPs in an Asian (Japanese) population (n = 523) comprising solely brown-eyed individuals. Comparison o...
Source: Cell Biochemistry and Function - May 26, 2009 Category: Biochemistry Authors: Reiko Iida, Misuzu Ueki, Haruo Takeshita, Junko Fujihara, Tamiko Nakajima, Yoshihiko Kominato, Masataka Nagao, Toshihiro Yasuda Source Type: journals

Age associated high level of major vault protein is p53 dependentEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, designed to elucidate the regulation of MVP in young and senescent HDFs, we examined the levels of transcriptional factors for the MVP gene, which revealed that among the putative transcriptional factors, p53 decreased only in young HDFs, but not in senescent HDFs in response to H2O2 treatment in the same mode as the expression of MVP. Moreover, the phosphorylation status of p53 increased only in senescent HDFs but not in young HDFs in response to H2O2 treatment. Therefore, we tested the possibility of MVP regulation by p53 status. MVP is upregulated in p53 over-expressing young HDFs, while MVP is downregula...
Source: Cell Biochemistry and Function - May 26, 2009 Category: Biochemistry Authors: Hong-Joo An, Sung-Jin Ryu, Sung-Young Kim, Hae-Ri Choi, Jun-Ho Chung, Sang-Chul Park Source Type: journals

TSH induces co-localization of TSH receptor and Na/K-ATPase in human erythrocytesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion our results are compatible with the existence, in erythrocytes, of a functional TSHR that interacts with Na/K-ATPase after TSH treatment, thus suggesting a novel cell signalling pathway, potentially active in local circulatory control. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - May 22, 2009 Category: Biochemistry Authors: Silvana Balzan, Renata Del Carratore, Giuseppina Nicolini, Francesca Forini, Valter Lubrano, Marcella Simili, Pier Alberto Benedetti, Giorgio Iervasi Source Type: journals

Are nociceptin(1-13)NH2 and its structural analogue [ORN9]nociceptin(1-13)NH2 able to affect brain antioxidant status in control and kainic acid-treated rats?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In-vivo effects of nociceptin (N/OFQ(1-13)NH2) on the levels of lipid peroxidation and cell enzyme (superoxide dismutase, glutathione peroxidase and glutathione reductase) and non-enzyme (glutathione) antioxidants in brain of control and kainic acid-treated rats were studied. N/OFQ(1-13)NH2 effects were compared with those of its structural analogue [Orn9]N/OFQ(1-13)NH2. Kainic acid (25 µg, i.c.v) increased the lipid peroxidation (4 and 24 h after kainic acid treatment) and decreased the glutathione level (1 h after kainic acid injection). We failed to find, any changes in antioxidant enzyme activities, independently of t...
Source: Cell Biochemistry and Function - May 8, 2009 Category: Biochemistry Authors: Elina Tzvetanova, Almira Pavlova, Albena Alexandrova, Galina Nenkova, Lubomir Petrov, Margarita Kirkova, Radoslav Girchev, Emilia Naydenova Source Type: journals

Association of a new intronic polymorphism of the SOD2 gene (G1677T) with cancerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
There is growing evidence of the correlation between cancer and reactive oxygen species (ROS), especially superoxide. Low expression levels of the Mn-superoxide dismutase (SOD2) enzyme have been reported in cancer patients. Genetic variation in the regulatory regions of the SOD2 gene may increase the risk of cancer. We identified a genetic variation (G1677T, rs2Y758Y339) in the vicinity of the enhancer region located in intron 2 of the SOD2 gene that creates a potential glucocorticoid responsive element, and developed an assay to screen DNA samples of 220 individuals (73 control, 59 prostate cancer survival individuals and...
Source: Cell Biochemistry and Function - April 30, 2009 Category: Biochemistry Authors: Daniel Hernandez-Saavedra, Joe M. McCord Source Type: journals

Characterization of pharmacogenetically relevant CYP2D6 and ABCB1 gene polymorphisms in a Portuguese population sampleEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study the frequency of the pharmacologically relevant CYP2D6*3, *4, *5, *6 allelic variants and gene duplication, and ABCB1 C1236T and C3435T gene polymorphisms and their haplotypes was determined in a population sample of 100 Portuguese healthy subjects. CYP2D6 allele frequencies were 1.4% (*3), 13.3% (*4), 2.8% (*5), 1.8% (*6) and 6.1% (gene duplication), with 5% of the individuals classified as PM and 8.4% as UM. The frequencies obtained for the non-functional alleles and for the CYP2D6 gene duplication are in agreement with other South European populations, and reinforce the previously suggested south/north gra...
Source: Cell Biochemistry and Function - April 29, 2009 Category: Biochemistry Authors: C. Correia, P. Santos, A. M. Coutinho, A. M. Vicente Source Type: journals

RetractionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
No Abstract. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - April 24, 2009 Category: Biochemistry Source Type: journals

Note from the EditorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
No Abstract. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - April 24, 2009 Category: Biochemistry Authors: Nigel Loveridge Source Type: journals