Autofluorescence is a biomarker of neural stem cell activation state
Cell Stem Cell. 2024 Mar 13:S1934-5909(24)00054-7. doi: 10.1016/j.stem.2024.02.011. Online ahead of print.ABSTRACTNeural stem cells (NSCs) must exit quiescence to produce neurons; however, our understanding of this process remains constrained by the technical limitations of current technologies. Fluorescence lifetime imaging (FLIM) of autofluorescent metabolic cofactors has been used in other cell types to study shifts in cell states driven by metabolic remodeling that change the optical properties of these endogenous fluorophores. Using this non-destructive, live-cell, and label-free strategy, we found that quiescent NSCs...
Source: Cell Stem Cell - March 23, 2024 Category: Stem Cells Authors: Christopher S Morrow Kelsey Tweed Sabina Farhadova Alex J Walsh Bo P Lear Avtar Roopra Ryan D Risgaard Payton C Klosa Zachary P Arndt Ella R Peterson Michelle M Chi Allison G Harris Melissa C Skala Darcie L Moore Source Type: research

Autofluorescence is a biomarker of neural stem cell activation state
Cell Stem Cell. 2024 Mar 13:S1934-5909(24)00054-7. doi: 10.1016/j.stem.2024.02.011. Online ahead of print.ABSTRACTNeural stem cells (NSCs) must exit quiescence to produce neurons; however, our understanding of this process remains constrained by the technical limitations of current technologies. Fluorescence lifetime imaging (FLIM) of autofluorescent metabolic cofactors has been used in other cell types to study shifts in cell states driven by metabolic remodeling that change the optical properties of these endogenous fluorophores. Using this non-destructive, live-cell, and label-free strategy, we found that quiescent NSCs...
Source: Cell Stem Cell - March 23, 2024 Category: Stem Cells Authors: Christopher S Morrow Kelsey Tweed Sabina Farhadova Alex J Walsh Bo P Lear Avtar Roopra Ryan D Risgaard Payton C Klosa Zachary P Arndt Ella R Peterson Michelle M Chi Allison G Harris Melissa C Skala Darcie L Moore Source Type: research

Autofluorescence is a biomarker of neural stem cell activation state
Cell Stem Cell. 2024 Mar 13:S1934-5909(24)00054-7. doi: 10.1016/j.stem.2024.02.011. Online ahead of print.ABSTRACTNeural stem cells (NSCs) must exit quiescence to produce neurons; however, our understanding of this process remains constrained by the technical limitations of current technologies. Fluorescence lifetime imaging (FLIM) of autofluorescent metabolic cofactors has been used in other cell types to study shifts in cell states driven by metabolic remodeling that change the optical properties of these endogenous fluorophores. Using this non-destructive, live-cell, and label-free strategy, we found that quiescent NSCs...
Source: Cell Stem Cell - March 23, 2024 Category: Stem Cells Authors: Christopher S Morrow Kelsey Tweed Sabina Farhadova Alex J Walsh Bo P Lear Avtar Roopra Ryan D Risgaard Payton C Klosa Zachary P Arndt Ella R Peterson Michelle M Chi Allison G Harris Melissa C Skala Darcie L Moore Source Type: research

Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Cell Stem Cell. 2024 Mar 12:S1934-5909(24)00053-5. doi: 10.1016/j.stem.2024.02.010. Online ahead of print.ABSTRACTFor precise genome editing via CRISPR/homology-directed repair (HDR), effective and safe editing of long-term engrafting hematopoietic stem cells (LT-HSCs) is required. The impact of HDR on true LT-HSC clonal dynamics in a relevant large animal model has not been studied. To track the output and clonality of HDR-edited cells and to provide a comparison to lentivirally transduced HSCs in vivo, we developed a competitive rhesus macaque (RM) autologous transplantation model, co-infusing HSCs transduced with a barc...
Source: Cell Stem Cell - March 20, 2024 Category: Stem Cells Authors: Byung-Chul Lee Ashley Gin Chuanfeng Wu Komudi Singh Max Grice Ryland Mortlock Diana Abraham Xing Fan Yifan Zhou Aisha AlJanahi Uimook Choi Suk See DeRavin Taehoon Shin Sogun Hong Cynthia E Dunbar Source Type: research

Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Cell Stem Cell. 2024 Mar 12:S1934-5909(24)00053-5. doi: 10.1016/j.stem.2024.02.010. Online ahead of print.ABSTRACTFor precise genome editing via CRISPR/homology-directed repair (HDR), effective and safe editing of long-term engrafting hematopoietic stem cells (LT-HSCs) is required. The impact of HDR on true LT-HSC clonal dynamics in a relevant large animal model has not been studied. To track the output and clonality of HDR-edited cells and to provide a comparison to lentivirally transduced HSCs in vivo, we developed a competitive rhesus macaque (RM) autologous transplantation model, co-infusing HSCs transduced with a barc...
Source: Cell Stem Cell - March 20, 2024 Category: Stem Cells Authors: Byung-Chul Lee Ashley Gin Chuanfeng Wu Komudi Singh Max Grice Ryland Mortlock Diana Abraham Xing Fan Yifan Zhou Aisha AlJanahi Uimook Choi Suk See DeRavin Taehoon Shin Sogun Hong Cynthia E Dunbar Source Type: research

Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Cell Stem Cell. 2024 Mar 12:S1934-5909(24)00053-5. doi: 10.1016/j.stem.2024.02.010. Online ahead of print.ABSTRACTFor precise genome editing via CRISPR/homology-directed repair (HDR), effective and safe editing of long-term engrafting hematopoietic stem cells (LT-HSCs) is required. The impact of HDR on true LT-HSC clonal dynamics in a relevant large animal model has not been studied. To track the output and clonality of HDR-edited cells and to provide a comparison to lentivirally transduced HSCs in vivo, we developed a competitive rhesus macaque (RM) autologous transplantation model, co-infusing HSCs transduced with a barc...
Source: Cell Stem Cell - March 20, 2024 Category: Stem Cells Authors: Byung-Chul Lee Ashley Gin Chuanfeng Wu Komudi Singh Max Grice Ryland Mortlock Diana Abraham Xing Fan Yifan Zhou Aisha AlJanahi Uimook Choi Suk See DeRavin Taehoon Shin Sogun Hong Cynthia E Dunbar Source Type: research

Lung repair and regeneration: Advanced models and insights into human disease
Cell Stem Cell. 2024 Mar 11:S1934-5909(24)00052-3. doi: 10.1016/j.stem.2024.02.009. Online ahead of print.ABSTRACTThe respiratory system acts as both the primary site of gas exchange and an important sensor and barrier to the external environment. The increase in incidences of respiratory disease over the past decades has highlighted the importance of developing improved therapeutic approaches. This review will summarize recent research on the cellular complexity of the mammalian respiratory system with a focus on gas exchange and immunological defense functions of the lung. Different models of repair and regeneration will...
Source: Cell Stem Cell - March 16, 2024 Category: Stem Cells Authors: Maria C Basil Konstantinos-Dionysios Alysandratos Darrell N Kotton Edward E Morrisey Source Type: research

Lung repair and regeneration: Advanced models and insights into human disease
Cell Stem Cell. 2024 Mar 11:S1934-5909(24)00052-3. doi: 10.1016/j.stem.2024.02.009. Online ahead of print.ABSTRACTThe respiratory system acts as both the primary site of gas exchange and an important sensor and barrier to the external environment. The increase in incidences of respiratory disease over the past decades has highlighted the importance of developing improved therapeutic approaches. This review will summarize recent research on the cellular complexity of the mammalian respiratory system with a focus on gas exchange and immunological defense functions of the lung. Different models of repair and regeneration will...
Source: Cell Stem Cell - March 16, 2024 Category: Stem Cells Authors: Maria C Basil Konstantinos-Dionysios Alysandratos Darrell N Kotton Edward E Morrisey Source Type: research

Lung repair and regeneration: Advanced models and insights into human disease
Cell Stem Cell. 2024 Mar 11:S1934-5909(24)00052-3. doi: 10.1016/j.stem.2024.02.009. Online ahead of print.ABSTRACTThe respiratory system acts as both the primary site of gas exchange and an important sensor and barrier to the external environment. The increase in incidences of respiratory disease over the past decades has highlighted the importance of developing improved therapeutic approaches. This review will summarize recent research on the cellular complexity of the mammalian respiratory system with a focus on gas exchange and immunological defense functions of the lung. Different models of repair and regeneration will...
Source: Cell Stem Cell - March 16, 2024 Category: Stem Cells Authors: Maria C Basil Konstantinos-Dionysios Alysandratos Darrell N Kotton Edward E Morrisey Source Type: research

Lung repair and regeneration: Advanced models and insights into human disease
Cell Stem Cell. 2024 Mar 11:S1934-5909(24)00052-3. doi: 10.1016/j.stem.2024.02.009. Online ahead of print.ABSTRACTThe respiratory system acts as both the primary site of gas exchange and an important sensor and barrier to the external environment. The increase in incidences of respiratory disease over the past decades has highlighted the importance of developing improved therapeutic approaches. This review will summarize recent research on the cellular complexity of the mammalian respiratory system with a focus on gas exchange and immunological defense functions of the lung. Different models of repair and regeneration will...
Source: Cell Stem Cell - March 16, 2024 Category: Stem Cells Authors: Maria C Basil Konstantinos-Dionysios Alysandratos Darrell N Kotton Edward E Morrisey Source Type: research

Promises and challenges of organoids: From humanized to human derived
Cell Stem Cell. 2024 Mar 7;31(3):281-282. doi: 10.1016/j.stem.2024.02.002.ABSTRACTKastenschmidt et al.1 present a groundbreaking organoid culture model for follicular lymphoma, which is capable of maintaining stable compositions of B and T cells. This model is utilized in testing bispecific antibodies in effective killing of tumor B cells with the activation of T cells.PMID:38458173 | DOI:10.1016/j.stem.2024.02.002 (Source: Cell Stem Cell)
Source: Cell Stem Cell - March 8, 2024 Category: Stem Cells Authors: Wenyan Wang Yang-Xin Fu Source Type: research

Development has the answer: Unraveling psychiatric disorders via thalamocortical organoids
Cell Stem Cell. 2024 Mar 7;31(3):283-284. doi: 10.1016/j.stem.2024.02.008.ABSTRACTDissecting the role of the thalamus in neuropsychiatric disorders requires new models to analyze complex genetic interactions. In this issue of Cell Stem Cell, Shin et al. use patient-derived thalamocortical organoids to investigate 22q11.2 microdeletion impact on thalamic development, revealing significant transcriptional dysregulation linked to psychiatric disorders.PMID:38458174 | DOI:10.1016/j.stem.2024.02.008 (Source: Cell Stem Cell)
Source: Cell Stem Cell - March 8, 2024 Category: Stem Cells Authors: Eduardo Leyva-D íaz Emily S Wilson Guillermina L ópez-Bendito Source Type: research

From suppressor to enhancer: IL-10's alternative role in CAR-T cell therapies against solid tumors
Cell Stem Cell. 2024 Mar 7;31(3):285-287. doi: 10.1016/j.stem.2024.02.003.ABSTRACTAiming to improve the effector function of CAR-T cells, Zhao et al.1 report that IL-10 metabolically reprograms CAR-T cells, and this promotes their effectiveness against solid tumors and challenges IL-10's perceived role as merely immunosuppressive. This simple but promising strategy fosters durable immune memory and eagerly awaits validation in clinical trials.PMID:38458175 | DOI:10.1016/j.stem.2024.02.003 (Source: Cell Stem Cell)
Source: Cell Stem Cell - March 8, 2024 Category: Stem Cells Authors: Yuwei Huang Haopeng Wang Source Type: research

KOLF2.1J iPSCs carry CNVs associated with neurodevelopmental disorders
Cell Stem Cell. 2024 Mar 7;31(3):288-289. doi: 10.1016/j.stem.2024.02.007.NO ABSTRACTPMID:38458176 | DOI:10.1016/j.stem.2024.02.007 (Source: Cell Stem Cell)
Source: Cell Stem Cell - March 8, 2024 Category: Stem Cells Authors: Carolina Gracia-Diaz Jonathan E Perdomo Munir E Khan Thomas Roule Brianna L Disanza Gregory G Cajka Sunyimeng Lei Alyssa L Gagne Jean Ann Maguire Ophir Shalem Elizabeth J Bhoj Rebecca C Ahrens-Nicklas Deborah L French Ethan M Goldberg Kai Wang Joseph T Gl Source Type: research

Large structural variants in KOLF2.1J are unlikely to compromise neurological disease modeling
Cell Stem Cell. 2024 Mar 7;31(3):290-291. doi: 10.1016/j.stem.2024.02.006.NO ABSTRACTPMID:38458177 | DOI:10.1016/j.stem.2024.02.006 (Source: Cell Stem Cell)
Source: Cell Stem Cell - March 8, 2024 Category: Stem Cells Authors: Mallory Ryan Justin A McDonough Michael E Ward Mark R Cookson William C Skarnes Florian T Merkle Source Type: research