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99 records returned

Simulated diving after heat stress potentiates the induction of heat shock protein 70 and elevates glutathione in human endothelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Heat stress prior to diving has been shown to confer protection against endothelial damage due to decompression sickness. Several lines of evidence indicate a relation between such protection and the heat shock protein (HSP)70 and HSP90 and the major cellular red-ox determinant, glutathione (GSH). The present study has used human endothelial cells as a model system to investigate how heat stress and simulated diving affect these central cellular defense molecules. The results demonstrated for the first time that a simulated dive at 2.6 MPa (26 bar) had a potentiating effect on the heat-induced expression of HSP70, incr...
Source: Cell Stress and Chaperones - November 19, 2009 Category: Cytology Authors: Djurhuus R, Nossum V, Lundsett N, Hovin W, Svardal AM, Havnes MB, Fismen L, Hjelde A, Brubakk AO Tags: Cell Stress Chaperones Source Type: journals

Why proteins without an alpha-crystallin domain should not be included in the human small heat shock protein family HSPB.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The presence of an alpha-crystallin domain documents the evolutionary relatedness of the ubiquitous family of small heat shock proteins. Sequence and three-dimensional structure provide no evidence for the presence of such a domain in HSPC034, recently proposed as the 11th member of the human HSPB family. Also, phylogenetic analyses detect no relationship between HSPC034 and the human HSPB1-10 sequences. Arguments are provided as to why inclusion in the HSPB family of proteins like HSPC034, which resemble small heat shock proteins in being heat-inducible and having chaperone-like properties and a low monomeric mass, bu...
Source: Cell Stress and Chaperones - November 18, 2009 Category: Cytology Authors: Kappé G, Boelens WC, de Jong WW Tags: Cell Stress Chaperones Source Type: journals

Neutrophil Gelatinase-Associated Lipocalin induces the expression of heme oxygenase-1 and superoxide dismutase (1, 2).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this report we intended to test the effect of lipocalin-2 on the expression of heme oxygenase ((1, 2)) and superoxide dismutase ((1, 2)) which are two strong antioxidants. NGAL was cloned to pcDNA3.1 plasmid by using genetic engineering method. The recombinant vector was transfected to CHO and HEK293T to establish stable cell expressing NGAL and the expression of HO-1, 2 and SOD(1, 2) were compared with appropriate controls by RT-PCR and western blot. On the other hand, expression of NGAL was suppressed by siRNA transfection in order to study the effect of lipocalin-2 on mentioned genes/proteins. The results showed that...
Source: Cell Stress and Chaperones - November 11, 2009 Category: Cytology Authors: Bahmani P, Halabian R, Rouhbakhsh M, Roushandeh AM, Masroori N, Ebrahimi M, Samadikuchaksaraei A, Shokrgozar MA, Roudkenar MH Tags: Cell Stress Chaperones Source Type: journals

The anti-necrosis role of hypoxic preconditioning after acute anoxia is mediated by aldose reductase and sorbitol pathway in PC12 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we examined the effect of HP (10% O(2), 48 h) on necrosis induced by AA (0% O(2), 24 h) in PC12 cells. We found that HP delayed the regulatory volume decrease and reduced cell swelling after 24 h of exposure to AA. Since aldose reductase (AR) is involved in cell volume regulation, we detected AR mRNA expression with reverse transcription-polymerase chain reaction (RT-PCR) techniques. The AR mRNA level was dramatically elevated by HP. Furthermore, an HP-induced decrease in cell injury was reversed by berberine chloride (BB), the inhibitor of AR. In addition, sorbitol synthesized from glucose catalyzed by AR i...
Source: Cell Stress and Chaperones - November 10, 2009 Category: Cytology Authors: Wu LY, Ma ZM, Fan XL, Zhao T, Liu ZH, Huang X, Li MM, Xiong L, Zhang K, Zhu LL, Fan M Tags: Cell Stress Chaperones Source Type: journals

Plasma cell differentiation initiates a limited ER stress response by specifically suppressing the PERK-dependent branch of the unfolded protein response.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In response to terminal differentiation signals that enable B cells to produce vast quantities of antibodies, a dramatic expansion of the secretory pathway and a corresponding increase in the molecular chaperones and folding enzymes that aid and monitor immunoglobulin synthesis occurs. Recent studies reveal that the unfolded protein response (UPR), which is normally activated by endoplasmic reticulum (ER) stress, plays a critical role in this process. Although B cells activate all three branches of the UPR in response to pharmacological inducers of the pathway, plasma cell differentiation elicits only a partial UPR in ...
Source: Cell Stress and Chaperones - November 8, 2009 Category: Cytology Authors: Ma Y, Shimizu Y, Mann MJ, Jin Y, Hendershot LM Tags: Cell Stress Chaperones Source Type: journals

Serum heat shock protein 27 antigen and antibody levels appear to be related to the macrovascular complications associated with insulin resistance: a pilot study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, Hsp27 and its antibody concentrations appear to relate to the presence of cardiovascular complications in patients with GI. PMID: 19882236 [PubMed - as supplied by publisher] (Source: Cell Stress and Chaperones)
Source: Cell Stress and Chaperones - October 31, 2009 Category: Cytology Authors: Pengiran Burut DF, Borai A, Livingstone C, Ferns G Tags: Cell Stress Chaperones Source Type: journals

The pivotal role of the beta7 strand in the intersubunit contacts of different human small heat shock proteins.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human alphaB-crystallin and small heat shock proteins HspB6 and HspB8 were mutated so that all endogenous Cys residues were replaced by Ser and the single Cys residue was inserted in a position homologous to that of Cys137 of human HspB1, i.e. in a position presumably located in the central part of beta7 strand of the alpha-crystallin domain. The secondary, tertiary, and quaternary structures of thus obtained Cys-mutants as well as their chaperone-like activity were similar to those of their wild-type counterparts. Mild oxidation of Cys-mutants leads to formation of disulfide bond crosslinking neighboring monomers thus...
Source: Cell Stress and Chaperones - October 24, 2009 Category: Cytology Authors: Mymrikov EV, Bukach OV, Seit-Nebi AS, Gusev NB Tags: Cell Stress Chaperones Source Type: journals

Polymorphisms in the HSP90AA1 5' flanking region are associated with scrapie incubation period in sheep.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Susceptibility to scrapie is mainly controlled by point mutations at the PRNP locus. However, additional quantitative trait loci (QTL) have been identified across the genome including a region in OAR18. The gene which encodes the inducible form of the cytoplasmic Hsp90 chaperone (HSP90AA1) maps within this region and seems to be associated with the resistance/susceptibility to scrapie in sheep. Here, we have analyzed several polymorphisms which were previously described in the ovine HSP90AA1 5' flanking region and in intron 10 in two naturally scrapie infected Romanov sheep populations. First, we have studied 58 ARQ/VR...
Source: Cell Stress and Chaperones - October 17, 2009 Category: Cytology Authors: Marcos-Carcavilla A, Moreno C, Serrano M, Laurent P, Cribiu EP, Andréoletti O, Ruesche J, Weisbecker JL, Calvo JH, Moazami-Goudarzi K Tags: Cell Stress Chaperones Source Type: journals

Proteasome inhibition induces hsp30 and hsp70 gene expression as well as the acquisition of thermotolerance in Xenopus laevis A6 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we report that proteasome inhibitors, lactacystin and carbobenzoxy-L: -leucyl-L: -leucyl-L: -leucinal (MG132), induced the accumulation of ubiquitinated proteins as well as a dose- and time-dependent increase in the relative levels of heat shock protein (HSP)30 and HSP70 and their respective mRNAs in Xenopus laevis A6 kidney epithelial cells. In A6 cells recovering from MG132 exposure, HSP30 and HSP70 levels were still elevated after 24 h but decreased substantially after 48 h. The activation of heat shock factor 1 (HSF1) may be involved in MG132-induced hsp gene expression in A6 cells since KNK437, a HSF1 i...
Source: Cell Stress and Chaperones - October 17, 2009 Category: Cytology Authors: Young JT, Heikkila JJ Tags: Cell Stress Chaperones Source Type: journals

Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this study is to investigate the expression and distribution of heat shock protein 70 (Hsp70) in the intestine of intrauterine growth retardation (IUGR) piglets. Samples from the duodenum, prejejunum, distal jejunum, ileum, and colon of IUGR and normal-body-weight (NBW) piglets were collected at birth. The results indicated that the body and intestine weight of IUGR piglets were significantly lower than NBW piglets. The villus height and villus/crypt ratio in jejunum and ileum of IUGR piglets were significantly reduced compared to NBW piglets. These results indicated that IUGR causes abnormal gastrointesti...
Source: Cell Stress and Chaperones - October 14, 2009 Category: Cytology Authors: Zhong X, Wang T, Zhang X, Li W Tags: Cell Stress Chaperones Source Type: journals

Circulating heat shock protein 70 (HSPA1A) in normal and pathological pregnancies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Heat shock proteins (Hsps) are ubiquitous and phylogenetically conserved molecules. They are usually considered to be intracellular proteins with molecular chaperone and cytoprotective functions. However, Hsp70 (HSPA1A) is present in the peripheral circulation of healthy nonpregnant and pregnant individuals. In normal pregnancy, circulating Hsp70 levels are decreased, and show a positive correlation with gestational age and an inverse correlation with maternal age. The capacity of extracellular Hsp70 to elicit innate and adaptive proinflammatory (Th1-type) immune responses might be harmful in pregnancy and may lead to ...
Source: Cell Stress and Chaperones - October 11, 2009 Category: Cytology Authors: Molvarec A, Tamási L, Losonczy G, Madách K, Prohászka Z, Rigó J Tags: Cell Stress Chaperones Source Type: journals

The topical antimicrobial zinc pyrithione is a heat shock response inducer that causes DNA damage and PARP-dependent energy crisis in human skin cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The differentiated epidermis of human skin serves as an essential barrier against environmental insults from physical, chemical, and biological sources. Zinc pyrithione (ZnPT) is an FDA-approved microbicidal agent used worldwide in clinical antiseptic products, over-the-counter topical antimicrobials, and cosmetic consumer products including antidandruff shampoos. Here we demonstrate for the first time that cultured primary human skin keratinocytes and melanocytes display an exquisite vulnerability to nanomolar concentrations of ZnPT resulting in pronounced induction of heat shock response gene expression and impaired ...
Source: Cell Stress and Chaperones - October 6, 2009 Category: Cytology Authors: Lamore SD, Cabello CM, Wondrak GT Tags: Cell Stress Chaperones Source Type: journals

Characterization of the shsp genes in Drosophila buzzatii and association between the frequency of Valine mutations in hsp23 and climatic variables along a longitudinal gradient in Australia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The small heat shock gene (shsp) cluster of Drosophila buzzatii was sequenced and the gene order and DNA sequence were compared with those of the shsps in Drosophila melanogaster. The D. buzzatii shsp cluster contains an inversion and a duplication of hsp26. A phylogenetic tree was constructed based on hsp26 genes from several Drosophila species of the Sophophora and Drosophila subgenera. The tree shows first a separation of the Sophophora and the Drosophila subgenera and then the Drosophila subgenus is divided into the Hawaiian Drosophila and the repleta/virilis groups. Only the latter contain a duplicated hsp26. Comp...
Source: Cell Stress and Chaperones - October 5, 2009 Category: Cytology Authors: Frydenberg J, Barker JS, Loeschcke V Tags: Cell Stress Chaperones Source Type: journals

Increased circulating heat shock protein 70 levels in pregnant asthmatics.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, this study proves an elevation of circulating Hsp70 levels during asthmatic pregnancy compared to healthy pregnant women. However, further studies are warranted to determine the role of circulating Hsp70 in the pathogenesis of maternal and perinatal complications of asthma in pregnancy. PMID: 19777374 [PubMed - as supplied by publisher] (Source: Cell Stress and Chaperones)
Source: Cell Stress and Chaperones - September 23, 2009 Category: Cytology Authors: Tamási L, Bohács A, Tamási V, Stenczer B, Prohászka Z, Rigó J, Losonczy G, Molvarec A Tags: Cell Stress Chaperones Source Type: journals

Versatility of the small heat shock protein HSPB6 (Hsp20).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The recently published review by Dreiza et al. (Cell Stress and Chaperones DOI 10.1007/s12192-0090127-8 ) dealing with the functional role of HSPB6 in muscle regulation is critically analyzed. Published data indicate that the chaperone-like activity of HSPB6 is comparable with that of HSPB5 and that phosphorylation of HSPB6 does not affect its oligomeric structure. Different hypotheses concerning the molecular mechanisms of HSPB6 action on smooth muscle contraction and on the reorganization of the cytoskeleton are compared, and it is concluded that although HSPB6 is not a genuine actin-binding protein, it can affect th...
Source: Cell Stress and Chaperones - September 23, 2009 Category: Cytology Authors: Seit-Nebi AS, Gusev NB Tags: Cell Stress Chaperones Source Type: journals

First cellular approach of the effects of global warming on groundwater organisms: a study of the HSP70 gene expression.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Whereas the consequences of global warming at population or community levels are well documented, studies at the cellular level are still scarce. The study of the physiological or metabolic effects of such small increases in temperature (between +2 degrees C and +6 degrees C) is difficult because they are below the amplitude of the daily or seasonal thermal variations occurring in most environments. In contrast, subterranean biotopes are highly thermally buffered (+/-1 degrees C within a year), and underground water organisms could thus be particularly well suited to characterise cellular responses of global warming. T...
Source: Cell Stress and Chaperones - September 23, 2009 Category: Cytology Authors: Colson-Proch C, Morales A, Hervant F, Konecny L, Moulin C, Douady CJ Tags: Cell Stress Chaperones Source Type: journals

PEST sequences mediate heat shock factor 2 turnover by interacting with the Cul3 subunit of the Cul3-RING ubiquitin ligase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cullin-RING ubiquitin ligases promote the polyubiquitination and degradation of many important cellular proteins, which previous studies indicated can be targeted for degradation via interaction with BTB domain-containing subunits of this E3 ligase complex. PEST domains are known to promote the degradation of proteins that contain them. However, the molecular mechanism by which PEST sequences promote degradation of these proteins is not understood. Here we show that the PEST sequences of a short-lived protein called HSF2 interact with Cullin3, a subunit of a Cullin-RING E3 ubiquitin ligase, and that this interaction me...
Source: Cell Stress and Chaperones - September 18, 2009 Category: Cytology Authors: Xing H, Hong Y, Sarge KD Tags: Cell Stress Chaperones Source Type: journals

Nucleolin/C23 is a negative regulator of hydrogen peroxide-induced apoptosis in HUVECs.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nucleolin plays important roles in chromatin structure, rDNA transcription, rRNA maturation, nucleocytoplasmic transport, and ribosome assembly. Although it has been shown to be anti-apoptotic, the underlying mechanisms remain unclear. In the current study, we first examined endogenous nucleolin expression in response to oxidative stress-induced apoptosis in human umbilical vascular endothelial cells (HUVECs). Flow cytometry and caspase activity assays showed that H(2)O(2) treatment caused apoptosis of the cells; reverse-transcription polymerase chain reaction and Western blotting revealed the downregulation of nucleol...
Source: Cell Stress and Chaperones - September 12, 2009 Category: Cytology Authors: Zhang B, Wang H, Jiang B, Liang P, Liu M, Deng G, Xiao X Tags: Cell Stress Chaperones Source Type: journals

Caught with their PAMPs down? The extracellular signalling actions of molecular chaperones are not due to microbial contaminants.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In recent years, it has been hypothesised that a new signalling system may exist in vertebrates in which secreted molecular chaperones form a dynamic continuum between the cellular stress response and corresponding homeostatic physiological mechanisms. This hypothesis seems to be supported by the finding that many molecular chaperones are released from cells and act as extracellular signals for a range of cells. However, this nascent field of biological research seems to suffer from an excessive criticism that the biological activities of molecular chaperones are due to undefined components of the microbial expression ...
Source: Cell Stress and Chaperones - September 3, 2009 Category: Cytology Authors: Henderson B, Calderwood SK, Coates AR, Cohen I, van Eden W, Lehner T, Pockley AG Tags: Cell Stress Chaperones Source Type: journals

Protective effect of carbon monoxide pre-conditioning on LPS-induced endothelial cell stress.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, CO treatment seems to provide a good pre-conditioning for the prevention of LPS-induced endothelial injury. PMID: 19693705 [PubMed - as supplied by publisher] (Source: Cell Stress and Chaperones)
Source: Cell Stress and Chaperones - August 19, 2009 Category: Cytology Authors: Bernardini C, Zannoni A, Bacci ML, Forni M Tags: Cell Stress Chaperones Source Type: journals

KLF4 is a novel regulator of the constitutively expressed HSP90.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Krüppel-like factor 4 (KLF4) is a zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, and differentiation. But little is known about the regulation of KLF4 on the expression of HSP90 (HSP84 and HSP86). In the current study, overexpression of KLF4 was firstly identified to promote the basal expression of HSP90 (HSP84 and HSP86) but not the inducible expression in the C2C12 cells and RAW264.7 cells. Conversely, KLF4 inhibition by antisense oligonucleotides markedly decreased the constitutive expression of HSP90 (HSP84 and HSP86). Here, we also presented data t...
Source: Cell Stress and Chaperones - August 10, 2009 Category: Cytology Authors: Liu Y, Liu M, Liu J, Zhang H, Tu Z, Xiao X Tags: Cell Stress Chaperones Source Type: journals

A protocol comparison for the analysis of heat shock protein A1B +A1538G SNP.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Heat shock proteins act as molecular chaperones, assist in peptide maturation, and transport nascent peptides across membranes. One commonly studied single nucleotide polymorphism (SNP) for one of the proteins is HSPA1B (+A1538G). However, several studies of this polymorphism have failed to achieve Hardy-Weinberg equilibrium (HWE) for their sample. We compared various published procedures for analyzing the HSPA1B +A1538G SNP and report reasons for HWE discrepancies. Samples from 141 apparently healthy, physically active, volunteers (99 men and 42 women) were analyzed. The first protocol, initially described by Schr...
Source: Cell Stress and Chaperones - August 5, 2009 Category: Cytology Authors: Contreras-Sesvold CL, Sambuughin N, Blokhin A, Deuster PA Tags: Cell Stress Chaperones Source Type: journals

Stress response in the ascidian Ciona intestinalis: transcriptional profiling of genes for the heat shock protein 70 chaperone system under heat stress and endoplasmic reticulum stress.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The genome of Ciona intestinalis contains eight genes for HSP70 superfamily proteins, 36 genes for J-proteins, a gene for a J-like protein, and three genes for BAG family proteins. To understand the stress responses of genes in the HSP70 chaperone system comprehensively, the transcriptional profiles of these 48 genes under heat stress and endoplasmic reticulum (ER) stress were studied using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Heat stress treatment increased the messenger RNA (mRNA) levels of six HSP70 superfamily genes, eight J-protein family genes, and two BAG family genes. In ...
Source: Cell Stress and Chaperones - July 22, 2009 Category: Cytology Authors: Fujikawa T, Munakata T, Kondo SI, Satoh N, Wada S Tags: Cell Stress Chaperones Source Type: journals

Participation of HSP27 in the antiapoptotic action of 17beta-estradiol in skeletal muscle cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Exposure to 17beta-estradiol prior to induction of apoptosis protects skeletal muscle cells against damage. The mechanism involved in this protective action of the hormone is poorly understood. In the present study, using the murine muscle cell line C2C12, evidence was obtained that inhibition of H(2)O(2)-induced apoptosis by the estrogen requires the participation of heat shock protein 27 (HSP27). Reverse transcriptase polymerase chain reaction, Western blot, and immunocytochemistry assays showed that 17beta-estradiol induces a time-dependent (5-60 min) increase in the expression of HSP27. In addition, in presence of ...
Source: Cell Stress and Chaperones - July 20, 2009 Category: Cytology Authors: Vasconsuelo A, Milanesi L, Boland R Tags: Cell Stress Chaperones Source Type: journals

Transcription of the Neurospora crassa 70-kDa class heat shock protein genes is modulated in response to extracellular pH changes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we show that the N. crassa hsp70-1 (NCU09602.3) and hsp70-2 (NCU08693.3) genes are preferentially expressed in an acidic milieu after 15 h of cell growth in sufficient phosphate at 30 degrees C. No significant accumulation of these transcripts was detected at alkaline pH values. Both genes accumulated to a high level in mycelia that were incubated for 1 h at 45 degrees C, regardless of the phosphate concentration and extracellular pH changes. Transcription of the hsp70-1 and hsp70-2 genes was dependent on the pacC (+) background in mycelia cultured under optimal growth conditions or at 45 degrees C. The pacC...
Source: Cell Stress and Chaperones - July 17, 2009 Category: Cytology Authors: Squina FM, Leal J, Cipriano VT, Martinez-Rossi NM, Rossi A Tags: Cell Stress Chaperones Source Type: journals

Thiolutin inhibits endothelial cell adhesion by perturbing Hsp27 interactions with components of the actin and intermediate filament cytoskeleton.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Thiolutin is a dithiole synthesized by Streptomyces sp. that inhibits endothelial cell adhesion and tumor growth. We show here that thiolutin potently inhibits developmental angiogenesis in zebrafish and vascular outgrowth from tissue explants in 3D cultures. Thiolutin is a potent and selective inhibitor of endothelial cell adhesion accompanied by rapid induction of HSPB1 (Hsp27) phosphorylation. The inhibitory effects of thiolutin on endothelial cell adhesion are transient, potentially due to a compensatory increase in Hsp27 protein levels. Accordingly, heat shock induction of Hsp27 limits the anti-adhesive activity o...
Source: Cell Stress and Chaperones - July 4, 2009 Category: Cytology Authors: Jia Y, Wu SL, Isenberg JS, Dai S, Sipes JM, Field L, Zeng B, Bandle RW, Ridnour LA, Wink DA, Ramchandran R, Karger BL, Roberts DD Tags: Cell Stress Chaperones Source Type: journals

Serum level of soluble 70-kD heat shock protein is associated with high mortality in patients with colorectal cancer without distant metastasis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Many findings indicate that measuring the serum concentration of soluble 70-kD heat shock protein (soluble HSP70) may provide important information in cardiovascular, inflammatory, and pregnancy-related diseases; however, only scarce data are available in cancer. Therefore, using a commercial ELISA kit, we measured soluble HSP70 concentration in the sera of 179 patients with colorectal cancer. We investigated the relationship between soluble HSP70 concentration and mortality, during 33.0 (24.4-44.0) months long follow-up. High (>1.65 pg/ml, median concentration) soluble HSP70 level was a significant (hazard ratio: 1...
Source: Cell Stress and Chaperones - July 3, 2009 Category: Cytology Authors: Kocsis J, Madaras B, Tóth EK, Füst G, Prohászka Z Tags: Cell Stress Chaperones Source Type: journals

The small heat shock protein, HSPB6, in muscle function and disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The small heat shock protein, HSPB6, is a 17-kDa protein that belongs to the small heat shock protein family. HSPB6 was identified in the mid-1990s when it was recognized as a by-product of the purification of HSPB1 and HSPB5. HSPB6 is highly and constitutively expressed in smooth, cardiac, and skeletal muscle and plays a role in muscle function. This review will focus on the physiologic and biochemical properties of HSPB6 in smooth, cardiac, and skeletal muscle; the putative mechanisms of action; and therapeutic implications. PMID: 19568960 [PubMed - as supplied by publisher] (Source: Cell Stress and Chaperones)
Source: Cell Stress and Chaperones - June 30, 2009 Category: Cytology Authors: Dreiza CM, Komalavilas P, Furnish EJ, Flynn CR, Sheller MR, Smoke CC, Lopes LB, Brophy CM Tags: Cell Stress Chaperones Source Type: journals

The role of p38alpha mitogen-activated protein kinase gene in the HELLP syndrome.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mitogen-activated protein kinase (MAPK) p38alpha was shown to be implicated in the organogenesis of the placenta, and such placental alteration is crucial for the development of hemolysis, elevated liver enzymes, and low platelets (HELLP) syndrome. We aimed to analyze for the first time human placental expression of MAPK p38alpha in pregnancies complicated by HELLP. The placental expression of MAPK p38alpha was investigated by semiquantitative polymerase chain reaction using cDNA extracted from placental tissue of 15 pregnancies with HELLP syndrome and 15 gestational age-matched controls. Seven patients with HELLP also...
Source: Cell Stress and Chaperones - June 29, 2009 Category: Cytology Authors: Corradetti A, Saccucci F, Emanuelli M, Vagnoni G, Cecati M, Sartini D, Giannubilo SR, Tranquilli AL Tags: Cell Stress Chaperones Source Type: journals

The mechanism whereby heat shock induces apoptosis depends on the innate sensitivity of cells to stress.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The cellular response to heat shock (HS) is a paradigm for many human diseases collectively known as "protein conformation diseases" in which the accumulation of misfolded proteins induces cell death. Here, we analyzed how cells having a different apoptotic threshold die subsequent to a treatment with HS. Cells with a low apoptotic threshold mainly induced apoptosis through activation of conventional stress kinase signaling pathways. By contrast, cells with a high apoptotic threshold also died by apoptosis but likely after the accumulation of heat-aggregated proteins as revealed by the formation of aggresomes in these ...
Source: Cell Stress and Chaperones - June 25, 2009 Category: Cytology Authors: Bellmann K, Charette SJ, Nadeau PJ, Poirier DJ, Loranger A, Landry J Tags: Cell Stress Chaperones Source Type: journals

LPS-induced cytokine levels are repressed by elevated expression of HSP70 in rats: possible role of NF-kappaB.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Heat shock protein (HSP)70 provides a spectrum of protection against any of a variety of stresses, preventing damage measured at the level of molecules, cells, as well as whole organism. We have previously reported that lipopolysaccharide (LPS)-induced lethality in rats is prevented by a previous exposure to a mild thermal stress and that a thermal stress sufficient to induce HSP70 expression in the liver is accompanied by an inhibition of endotoxin-mediated cytokines and modulation of febrile response. However, the effect of HSP70 upregulation on cytokine expression in animals is unknown. The aim of the present study ...
Source: Cell Stress and Chaperones - June 23, 2009 Category: Cytology Authors: Dokladny K, Lobb R, Wharton W, Ma TY, Moseley PL Tags: Cell Stress Chaperones Source Type: journals

Identification of a novel inducible cytosolic Hsp70 gene in Chinese shrimp Fenneropenaeus chinensis and comparison of its expression with the cognate Hsc70 under different stresses.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the full-length complementary DNA (cDNA) of a novel inducible cytosolic Hsp70 family member (FcHsp70) was cloned from Fenneropenaeus chinensis. FcHsp70 full-length cDNA consists of 2,511 bp with a 1,890-bp open reading frame encoding 629 amino acids. Three Hsp70 protein family signatures, IDLGTTYS, IIDLGGGTFDVSIL, and IVLVGGSTRIPKVQK, were found in the predicted FcHsp70 amino acid sequence. Phylogenetic analysis showed that FcHsp70 was categorized together with the inducible HSP70s reported in other crustaceans. Compared to the previously identified cognate Hsp70 (FcHsc70) in F. chinensis, the expression of ...
Source: Cell Stress and Chaperones - June 3, 2009 Category: Cytology Authors: Luan W, Li F, Zhang J, Wen R, Li Y, Xiang J Tags: Cell Stress Chaperones Source Type: journals

A SNP in the HSP90AA1 gene 5' flanking region is associated with the adaptation to differential thermal conditions in the ovine species.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, several polymorphisms in the gene encoding the inducible form of the cytoplasmic Hsp90 (HSP90AA1) were addressed in 24 sheep breeds reared in different climatic regions of Europe, Africa, and Asia. Significant differences in the genotype frequencies for a C/G single nucleotide polymorphism (SNP) located at position -660 in the HSP90AA1 5'flanking region were found between the different breeds. Regression analyses reflected significant correlations (from 0.41 to 0.62) between the alternative genotypes of this polymorphism and several climatic and geographic variables characteristic of the regions where these ...
Source: Cell Stress and Chaperones - June 3, 2009 Category: Cytology Authors: Marcos-Carcavilla A, Mutikainen M, González C, Calvo JH, Kantanen J, Sanz A, Marzanov NS, Pérez-Guzmán MD, Serrano M Tags: Cell Stress Chaperones Source Type: journals

Core temperature correlates with expression of selected stress and immunomodulatory genes in febrile patients with sepsis and noninfectious SIRS.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Environmental hyperthermia and exercise produce extensive changes in gene expression in human blood cells, but it is unknown whether this also happens during febrile-range hyperthermia. We tested the hypothesis that heat shock protein (HSP) and immunomodulatory stress gene expression correlate with fever in intensive care unit patients. Whole blood messenger RNA was obtained over consecutive days from 100 hospitalized patients suffering from sepsis or noninfectious systemic inflammatory response syndrome (SIRS) as defined by conventional criteria. The most abnormal body temperature in the preceding 24 h was recorded fo...
Source: Cell Stress and Chaperones - June 3, 2009 Category: Cytology Authors: Sonna LA, Hawkins L, Lissauer ME, Maldeis P, Towns M, Johnson SB, Moore R, Singh IS, Cowan MJ, Hasday JD Tags: Cell Stress Chaperones Source Type: journals

Extracellular chaperones modulate the effects of Alzheimer's patient cerebrospinal fluid on Abeta(1-42) toxicity and uptake.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Alzheimer's disease is characterised by the inappropriate death of brain cells and accumulation of the Abeta peptide in the brain. Thus, it is possible that there are fundamental differences between Alzheimer's disease patients and healthy individuals in their abilities to clear Abeta from brain fluid and to protect neurons from Abeta toxicity. In the present study, we examined (1) the cytotoxicity of Alzheimer's disease cerebrospinal fluid (CSF) compared to control CSF, (2) the ability of Alzheimer's disease and control CSF to protect cells from Abeta toxicity and to promote cell-mediated clearance of Abeta and lastly...
Source: Cell Stress and Chaperones - May 26, 2009 Category: Cytology Authors: Yerbury JJ, Wilson MR Tags: Cell Stress Chaperones Source Type: journals

Hsp70 expression and induction as a readout for detection of immune modulatory components in food.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Stress proteins such as heat shock proteins (Hsps) are up-regulated in cells in response to various forms of stress, like thermal and oxidative stress and inflammation. Hsps prevent cellular damage and increase immunoregulation by the activation of anti-inflammatory T-cells. Decreased capacity for stress-induced Hsp expression is associated with immune disorders. Thus, therapeutic boosting Hsp expression might restore or enhance cellular stress resistance and immunoregulation. Especially food- or herb-derived phytonutrients may be attractive compounds to restore optimal Hsp expression in response to stress. In the pres...
Source: Cell Stress and Chaperones - May 26, 2009 Category: Cytology Authors: Wieten L, van der Zee R, Goedemans R, Sijtsma J, Serafini M, Lubsen NH, van Eden W, Broere F Tags: Cell Stress Chaperones Source Type: journals

Experimental testing of a mathematical model relevant to the extrinsic pathway of apoptosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Apoptosis is a programmed cell death process, whose complexity led researchers to build mathematical models that could help to identify its crucial steps. In previous works, we theoretically analyzed and numerically simulated a model that describes a pathway from an external stimulus to caspase-3 activation. Here, the results of experiments performed on populations of synchronized cells treated with the inducer Apo2L/TRAIL are reported and are compared with model predictions. In particular, we have compared in vitro and in silico results relevant to the time evolutions of caspase-3 and caspase-8 activities, as well as ...
Source: Cell Stress and Chaperones - May 19, 2009 Category: Cytology Authors: Pace V, Bellizzi D, Giordano F, Panno ML, De Benedictis G Tags: Cell Stress Chaperones Source Type: journals

Monoclonal antibody to novel cell surface epitope on Hsc70 promotes morphogenesis of bile ducts in newborn rat liver.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We previously described a cell surface reactive monoclonal antibody, MAb OC.10, which recognizes an epitope shared by rat fetal liver ductal cells, hepatic progenitor cells, mature cholangiocytes, and hepatocellular carcinomas (HCC). Here, intrasplenic injection of MAb OC.10 into newborn rats was shown by immunofluorescence microscopy to strongly label intrahepatic bile ducts. Furthermore, the in situ labeling of intrahepatic cholangiocytes by injecting MAb OC.10 increased the number of intraportal and intralobular bile ducts with well-defined lumens when compared to IgM-injected control animals. The antigen for MAb OC...
Source: Cell Stress and Chaperones - May 4, 2009 Category: Cytology Authors: Mills DR, Haskell MD, Callanan HM, Flanagan DL, Brilliant KE, Yang D, Hixson DC Tags: Cell Stress Chaperones Source Type: journals

NGFI-B targets mitochondria and induces cardiomyocyte apoptosis in restraint-stressed rats by mediating energy metabolism disorder.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
NGFI-B/Nur77/TR3, originally identified as an immediate-early gene rapidly induced by serum and growth factors, is a member of the steroid hormone nuclear receptor superfamily with no identified endogenous ligand. NGFI-B induces apoptosis in a number of cell lineages exposed to proapoptotic stimuli by directly targeting the mitochondria, inducing cytochrome c release. The present study was designed to determine the role of NGFI-B in cardiomyocytes of restraint-stressed rats. The NGFI-B content was increased in mitochondria and reduced in plasma as apoptosis increased. Analysis showed that NGFI-B induces cardiomyocyte a...
Source: Cell Stress and Chaperones - May 2, 2009 Category: Cytology Authors: Wang X, Liu X, Kong R, Zhan R, Wang X, Leng X, Gong J, Duan M, Wang L, Wu L, Qian L Tags: Cell Stress Chaperones Source Type: journals

Activation of Hsp90/NOS and increased NO generation does not impair mitochondrial respiratory chain by competitive binding at cytochrome C Oxidase in low oxygen concentrations.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, by using bovine aortic endothelial cells, we demonstrate that the inhibitory effect of endogenously generated NO by nitric oxide synthase (NOS) activation, by either NOS stimulators or association with heat shock protein 90 (Hsp90), is significant only at high prevailing pO(2) through nitrosation of mitochondrial ETC complexes, but it does not inhibit the respiration by competitive binding at CcO at very low pO(2). ETC complexes activity measurements confirmed that significant reduction in complex IV activity was noticed at higher pO(2), but it was unaffected at low pO(2) in these cells. This was further ext...
Source: Cell Stress and Chaperones - May 1, 2009 Category: Cytology Authors: Presley T, Vedam K, Liu X, Zweier JL, Ilangovan G Tags: Cell Stress Chaperones Source Type: journals

Triggers of the HSP70 stress response: environmental responses and laboratory manipulation in an Antarctic marine invertebrate (Nacella concinna).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The Antarctic limpet, Nacella concinna, exhibits the classical heat shock response, with up-regulation of duplicated forms of the inducible heat shock protein 70 (HSP70) gene in response to experimental manipulation of seawater temperatures. However, this response only occurs in the laboratory at temperatures well in excess of any experienced in the field. Subsequent environmental sampling of inter-tidal animals also showed up-regulation of these genes, but at temperature thresholds much lower than those required to elicit a response in the laboratory. It was hypothesised that this was a reflection of the complexity of...
Source: Cell Stress and Chaperones - April 29, 2009 Category: Cytology Authors: Clark MS, Peck LS Tags: Cell Stress Chaperones Source Type: journals

Novel Hsp90 partners discovered using complementary proteomic approaches.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hsp90 is an essential eukaryotic molecular chaperone that stabilizes a large set of client proteins, many of which are involved in various cellular signaling pathways. The current list of Hsp90 interactors comprises about 200 proteins and this number is growing steadily. In this paper, we report on the application of three complementary proteomic approaches directed towards identification of novel proteins that interact with Hsp90. These methods are coimmunoprecipitation, pull down with biotinylated geldanamycin, and immobilization of Hsp90beta on sepharose. In all, this study led to the identification of 42 proteins, ...
Source: Cell Stress and Chaperones - April 25, 2009 Category: Cytology Authors: Tsaytler PA, Krijgsveld J, Goerdayal SS, Rüdiger S, Egmond MR Tags: Cell Stress Chaperones Source Type: journals

Ingestion of bacteria overproducing DnaK attenuates Vibrio infection of Artemia franciscana larvae.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Feeding of bacterially encapsulated heat shock proteins (Hsps) to invertebrates is a novel way to limit Vibrio infection. As an example, ingestion of Escherichia coli overproducing prokaryotic Hsps significantly improves survival of gnotobiotically cultured Artemia larvae upon challenge with pathogenic Vibrio campbellii. The relationship between Hsp accumulation and enhanced resistance to infection may involve DnaK, the prokaryotic equivalent to Hsp70, a major molecular chaperone in eukaryotic cells. In support of this proposal, heat-stressed bacterial strains LVS 2 (Bacillus sp.), LVS 3 (Aeromonas hydrophila), LVS 8 (...
Source: Cell Stress and Chaperones - April 16, 2009 Category: Cytology Authors: Sung YY, Dhaene T, Defoirdt T, Boon N, Macrae TH, Sorgeloos P, Bossier P Tags: Cell Stress Chaperones Source Type: journals

The atheroprotective properties of Hsp70: a role for Hsp70-endothelial interactions?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Although heat shock (stress) proteins are typically regarded as being exclusively intracellular molecules, it is now apparent that they can be released from cells in the absence of cellular necrosis. We and others have reported the presence of Hsp60 (HSPD1) and Hsp70 (HSPA1A) in the circulation of normal individuals and our finding that increases in carotid intima-media thicknesses (a measure of atherosclerosis) in subjects with hypertension at a 4-year follow-up are less prevalent in those having high serum Hsp70 (HSPA1A) levels at baseline suggests that circulating Hsp70 (HSPA1A) has atheroprotective effects. Given t...
Source: Cell Stress and Chaperones - April 8, 2009 Category: Cytology Authors: Pockley AG, Calderwood SK, Multhoff G Tags: Cell Stress Chaperones Source Type: journals

Reinvestigation of the effect of carbenoxolone on the induction of heat shock proteins.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we reinvestigated the effect of CBX on the induction of HSPs in HeLa and human neuroblastoma (A-172) cells. CBX clearly induced not only Hsp70 but also Hsp90 (HSPC1), Hsp40 (DNAJB1), and Hsp27 (HSPB1) at concentrations of 10 to 800 muM for 16 h incubation. At higher concentrations (more than 400 muM), however, CBX appeared to be toxic. Treatment of cells with CBX resulted in enhanced phosphorylation and acquisition of DNA-binding ability of heat shock transcription factor 1 (HSF1). Furthermore, characteristic HSF1 granules were formed in the nucleus, suggesting that the induction of HSPs by CBX is mediated b...
Source: Cell Stress and Chaperones - March 31, 2009 Category: Cytology Authors: Kawashima D, Asai M, Katagiri K, Takeuchi R, Ohtsuka K Tags: Cell Stress Chaperones Source Type: journals

The ambiguous role of the Na(+)-H (+) exchanger isoform 1 (NHE1) in leptin-induced oxidative stress in human monocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Leptin, a 16-kDa cytokine produced mainly by the adipose tissue, is known to increase energy expenditure while at the same time lowering food intake by acting directly on the hypothalamus. ObRb, the leptin receptor mostly involved in intracellular signaling, is expressed in a wide range of tissues, thus allowing leptin to affect a much broader diversity of biological processes. High concentrations of leptin are encountered in patients with hyperleptinemia, a condition which very often accompanies obesity and which is a direct result of leptin resistance. In the present study, moderate and high concentrations of leptin ...
Source: Cell Stress and Chaperones - March 20, 2009 Category: Cytology Authors: Konstantinidis D, Paletas K, Koliakos G, Kaloyianni M Tags: Cell Stress Chaperones Source Type: journals

Protective effect of betaine on changes in the levels of lysosomal enzyme activities in heart tissue in isoprenaline-induced myocardial infarction in Wistar rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Myocardial infarction is one of the most common manifestations of cardiovascular disease. In the present study, we investigated the protective effect of betaine, a potent lipotropic molecule, on changes in the levels of lysosomal enzymes and lipid peroxidation in isoprenaline-induced myocardial infarction in Wistar rats, an animal model of myocardial infarction in man. Male albino Wistar rats were pretreated with betaine (250 mg/kg body weight) daily for a period of 30 days. After the treatment period, isoprenaline (11 mg/100 g body weight) was intraperitoneally administered to rats at intervals of 24 h for 2 days. The...
Source: Cell Stress and Chaperones - March 18, 2009 Category: Cytology Authors: Ganesan B, Anandan R Tags: Cell Stress Chaperones Source Type: journals

Heat stress upregulates chaperone heat shock protein 70 and antioxidant manganese superoxide dismutase through reactive oxygen species (ROS), p38MAPK, and Akt.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chinese hamster lung fibroblasts V79 cells were treated with heat stress for 4 weeks with short duration (15 min) heat shock every alternate day in culture. It was observed that Hsp 70 and the antioxidant enzyme MnSOD became overexpressed during the chronic heat stress period. Both p38 MAPK and Akt became phosphorylated by chronic heat stress exposure. Simultaneous exposure to SB203580, a potent and specific p38MAPK inhibitor drastically inhibited the phosphorylation of p38MAPK and Akt. Furthermore, exposure to SB203580 also blocked the increase in Hsp70 and MnSOD levels and the elevated SOD activity brought about by c...
Source: Cell Stress and Chaperones - March 17, 2009 Category: Cytology Authors: Banerjee Mustafi S, Chakraborty PK, Dey RS, Raha S Tags: Cell Stress Chaperones Source Type: journals

Protein synthesis rates in Drosophila associate with levels of the hsr-omega nuclear transcript.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Transcripts of the Drosophila hsr-omega gene are known to interact with RNA processing factors and ribosomes and are postulated to aid in co-ordinating nuclear and cytoplasmic activities particularly in stressed cells. However, the significance of these interactions for physiological processes and in turn for whole-organism fitness remains an open question. Because hsr-omega's cellular expression characteristics suggest it may influence protein synthesis, and because both genotypic and expression variation of hsr-omega have been associated with thermotolerance, we characterised 30 lines for variation in the rates of pr...
Source: Cell Stress and Chaperones - March 12, 2009 Category: Cytology Authors: Johnson TK, Carrington LB, Hallas RJ, McKechnie SW Tags: Cell Stress Chaperones Source Type: journals

Cloning, characterization, and functional studies of a human 40-kDa catecholamine-regulated protein: implications in central nervous system disorders.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, these results show that we have cloned a splice variant of mortalin with a novel catecholamine binding function and that this chaperone-like protein may be neuroprotective in dopamine-related central nervous system disorders. PMID: 19280369 [PubMed - as supplied by publisher] (Source: Cell Stress and Chaperones)
Source: Cell Stress and Chaperones - March 12, 2009 Category: Cytology Authors: Gabriele J, Pontoriero GF, Thomas N, Thomson CA, Skoblenick K, Pristupa ZB, Mishra RK Tags: Cell Stress Chaperones Source Type: journals