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Transplantation of Mesenchymal Stem Cells exerts a greater long-term effect than Bone Marrow Mononuclear Cells in a chronic myocardial infarction model in rat.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Altogether, results show that MSC provides a long-term superior benefit than whole BM-MNC transplantation in a rat model of chronic MI. PMID: 19919732 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - November 16, 2009 Category: Cytology Authors: Mazo M, Gavira JJ, Abizanda G, Moreno C, Ecay M, Soriano M, Aranda P, Collantes M, Alegría E, Merino J, Peñuelas IN, Verdugo JM, Pelacho B, Prosper F Tags: Cell Transplant Source Type: journals

Immune tolerance induction by integrating innate and adaptive immune regulators.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A diversity of immune tolerance mechanisms have evolved to protect normal tissues from immune damage. Immune regulatory cells are critical contributors to peripheral tolerance. These regulatory cells, exemplified by the CD4Foxp3 regulatory T (Treg) cells and a recently identified population named myeloid-derived suppressor cells (MDSCs), regulate immune responses and limiting immune-mediated pathology. In a chronic inflammatory setting, such as allograft-directed immunity, there may be a dynamic "crosstalk" between the innate and adaptive immunomodulatory mechanisms for an integrated control of immune damage. CTLA4-B7-...
Source: Cell Transplantation - November 16, 2009 Category: Cytology Authors: Suzuki J, Ricordi C, Chen Z Tags: Cell Transplant Source Type: journals

Time-Dependent Migration of Systemically Delivered Bone Marrow Mesenchymal Stem Cells to the Infarcted Heart.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study the time course of homing and the body distribution of systemically delivered bone marrow mesenchymal stem cells (BM-MSCs) after myocardial infarction (MI) were evaluated. BM-MSCs were isolated from Wistar rats, expanded in vitro and their phenotypical characterization was performed by flow cytometer. Rats were randomly divided into three groups: control, sham MI, and MI. BM-MSCs (5x10) were labeled with mTc-HMPAO and injected through the tail vein 7 days after MI. Gamma camera imaging was performed at 5, 15, 30 and 60 min after cell inoculation. Due to the mTc short half-life, cell migration and location wer...
Source: Cell Transplantation - November 10, 2009 Category: Cytology Authors: Assis AC, Carvalho JL, Jacoby BA, Ferreira RL, Castanheira P, Diniz SO, Cardoso VN, Goes AM, Ferreira AJ Tags: Cell Transplant Source Type: journals

Hepatocyte Transplantation and the Differentiation Fate of Host Oval Cells in Acute Severe Hepatic Injury.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to determine the impacts of hepatocyte transplantation on the response and fate of oval cells that are activated to proliferate in acute severe hepatic injury. Retrorsine-plus- D-galactosamine (R+D-gal) treatment was used to induce acute hepatic injury and to elicit extensive activation of oval cells in male Dipeptidyl-peptidase-IV-deficient F344 rats. These rats were then randomized to receive wild-type hepatocyte transplantation or vehicle intraportally. The kinetics of oval cell response and their differentiation fate were analyzed. Results showed that oval cells were activated early and differentiated ...
Source: Cell Transplantation - November 10, 2009 Category: Cytology Authors: Yu CH, Chang MH, Chien CS, Chen YH, Chang MF, Chen HL Tags: Cell Transplant Source Type: journals

Transplantation of undifferentiated human mesenchymal stem cells protects against 6-hydroxydopamine neurotoxicity in the rat.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, our results suggest that grafted hMSCs exert neuroprotective effects against nigrostriatal degeneration induced by 6-OHDA. The mechanisms underlying this effect remain to be clarified, although it is likely that the acquisition of a glial phenotype by grafted hMSCs may lead to the release of pro-survival cytokines within the lesioned striatum. PMID: 19906332 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - November 10, 2009 Category: Cytology Authors: Blandini F, Cova L, Armentero MT, Zennaro E, Levandis G, Bossolasco P, Calzarossa C, Mellone M, Giuseppe B, Deliliers GL, Polli E, Nappi G, Silani V Tags: Cell Transplant Source Type: journals

International Placenta Stem Cell Society: Planting the Seed for Placenta Stem Cell Research.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
[no abstract]. PMID: 19906333 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - November 10, 2009 Category: Cytology Authors: Borlongan CV, Parolini O Tags: Cell Transplant Source Type: journals

Non-albumin Proteinuria in Islet Transplant Recipients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, high frequency of proteinuria was observed after ITx. However, it seems to be milder and have less impact on KF when albumin is not the major source of proteinuria. Prospective evaluation of proteinuria, including tubular function markers, should be performed to elucidate the mechanisms of kidney damage in this population. PMID: 19878623 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - October 29, 2009 Category: Cytology Authors: Leitão CB, Froud T, Cure P, Tharavanij T, Pileggi A, Ricordi C, Md RA Tags: Cell Transplant Source Type: journals

Making Stem Cells Infarct Avid.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSION: The binding of C2A-ESCs to moribund cardiomyocytes demonstrates that the retention of transplanted cells may be improved by conferring these cells with the ability to bind infarcted tissue. Once established, this novel approach may be applicable to other types of transplanted therapeutic cells. PMID: 19878624 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - October 29, 2009 Category: Cytology Authors: Zhao M, Barron MR, Li Z, Koprowski S, Hall CL, Lough J Tags: Cell Transplant Source Type: journals

Utility of telomerase-pot1 fusion protein in vascular tissue engineering.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
While advances in regenerative medicine and vascular tissue engineering have been substantial in recent years, important stumbling blocks remain. In particular, the limited lifespan of differentiated cells that are harvested from elderly human donors is an important limitation in many areas of regenerative medicine. Recently, a mutant of the human telomerase reverse transcriptase enzyme (TERT) was described, which is highly processive and elongates telomeres more rapidly than conventional telomerase. This mutant, called pot1-TERT, is a chimeric fusion between the DNA binding protein pot1 and TERT. Because pot1-TERT is ...
Source: Cell Transplantation - October 29, 2009 Category: Cytology Authors: Petersen TH, Hitchcock T, Muto A, Calle EA, Zhao L, Gong Z, Gui L, Dardik A, Bowles DE, Counter CM, Niklason LE Tags: Cell Transplant Source Type: journals

Labeling Human Embryonic Stem Cell Derived Cardiomyocytes with Indocyanine Green for Noninvasive Tracking with Optical Imaging: An FDA Compatible Alternative to Firefly Luciferase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human embryonic stem cell derived cardiomyocytes (hESC-CMs) have demonstrated the ability to improve myocardial function following transplantation into an ischemic heart; however the functional benefits are transient possibly due to poor cell retention. A diagnostic technique that could visualize transplanted hESC-CMs could help to optimize stem cell delivery techniques. Thus, the purpose of this study was to develop a labeling technique for hESCs and hESC-CMs with the FDA-approved contrast agent Indocyanine Green (ICG) for optical imaging (OI). hESCs were labeled with 0.5, 1.0, 2.0 and 2.5 mg/ml of ICG for 30, 45 and ...
Source: Cell Transplantation - October 29, 2009 Category: Cytology Authors: Boddington SE, Md TD, Md PJ, Schlieve CR, Mandrussow L, Phd DD, Phd HS, Ritner C, Md DG, Phd YL, Phd SZ, Phd HE Tags: Cell Transplant Source Type: journals

Enhanced Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells in Low Oxygen Environment Micropellet Cultures.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chondrogenesis of mesenchymal stem cells (MSCs) is typically induced when they are condensed into a single aggregate and exposed to transforming growth factor beta (TGF-beta). Hypoxia, like aggregation and TGF-beta delivery, may be crucial for complete chondrogenesis. However, the pellet dimensions and associated self-induced oxygen gradients of current chondrogenic methods may limit the effectiveness of in vitro differentiation and subsequent therapeutic uses. Here we describe the use of embryoid body-forming technology to produce microscopic aggregates of human bone marrow MSCs (BM-MSCs) for chondrogenesis. The use o...
Source: Cell Transplantation - October 29, 2009 Category: Cytology Authors: Markway BD, Tan GK, Brooke G, Hudson JE, Cooper-White JJ, Doran MR Tags: Cell Transplant Source Type: journals

A first semi-manual device for clinical intramuscular repetitive cell injections.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Intramuscular cell transplantation in humans requires so far meticulous repetitive cell-injections. Performed percutaneously with syringes operated manually, the procedure is very time-consuming and requires a lot of concentration to deliver the cells exactly in the required region. This becomes impractical and inaccurate for large volumes of muscle. In order to accelerate this task, to render it more precise and to perform injections more reproducible in large volumes of muscle, we developed a specific semi-manual device for intramuscular repetitive cell injections. Our prototype delivers very small quantities of cell...
Source: Cell Transplantation - October 29, 2009 Category: Cytology Authors: Richard PL, Gosselin C, Laliberté T, Paradis M, Goulet M, Tremblay JP, Skuk D Tags: Cell Transplant Source Type: journals

Use of lectins to enrich mouse ES-derived retinal progenitor cells for the purpose of transplantation therapy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Using the mouse ES cell line with green fluorescent protein knocked-in at the Rx locus (Rx-KI ES cell), we previously showed that photoreceptors can be efficiently obtained in defined culture conditions by enriching Rx-positive retinal progenitor cells. We aimed to explore a protocol applicable for non-Rx-labeled stem cell lines for subsequent enrichment of retinal photoreceptor precursors for transplantation. The Rx-KI ES cell line was differentiated according to the serum-free suspension conditions with serum-free Suspension /Dkk1/LeftyA/serum/activin method (SFEB/DLFA) described previously. Enrichment efficacy by ne...
Source: Cell Transplantation - October 8, 2009 Category: Cytology Authors: Mandai M, Ikeda H, Jin ZB, Iseki K, Ishigami C, Takahashi M Tags: Cell Transplant Source Type: journals

Controlled release of neurotrophin-3 and platelet derived growth factor from fibrin scaffolds containing neural progenitor cells enhances survival and differentiation into neurons in a subacute model of SCI.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report here that the combination of the NT-3, PDGF and fibrin scaffold (with or without HBDS) enhanced the total number of ESNPCs present in the spinal cord lesion 2 weeks after injury. In addition, the inclusion of the HBDS with growth factor resulted in an increase in the number of ESNPC-derived NeuN positive neurons. These results demonstrate the ability of fibrin scaffolds and the controlled release of growth factors to enhance the survival and differentiation of neural progenitor cells following transplantation into a SCI model. PMID: 19818206 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - October 8, 2009 Category: Cytology Authors: Johnson PJ, Tatara A, Shiu A, Sakiyama-Elbert SE Tags: Cell Transplant Source Type: journals

Both CD133+ cells and monocytes provide significant improvement for hindlimb ischemia, although they do not transdifferentiate into endothelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Our study shows that both purified CD133+ stem cells and myeloid mononuclear cells, either IM- or IV-administered, have similar neoangiogenic ability. Nevertheless, transdifferentiation into endothelial cells is not the mechanism responsible for their beneficial effect. PMID: 19818207 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - October 8, 2009 Category: Cytology Authors: Sanchez-Guijo FM, Oterino E, Barbado MV, Carrancio S, Lopez-Holgado N, Muntion S, Hernandez-Campo P, Sanchez-Abarca LI, Perez-Simon JA, Miguel JF, Briñon JG, Cañizo MC Tags: Cell Transplant Source Type: journals

Large-Scale Comparison of Liberase HI and Collagenase NB1 Utilized for Human Islet Isolation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
For more than a decade Liberase HI was commonly used as the standard enzyme blend for clinical human islet isolation until enforced replacement by collagenase NB1 (NB1). This change resulted initially in a reduction in islet isolation outcome and transplant activities worldwide. This retrospective study was initiated to compare the efficiency of NB1 premium grade with Liberase in 197 human islet isolations. All pancreata were processed between January 2006 and June 2008 utilizing the same procedures for isolation and quality assessment except the administration of preselected lots of either Liberase (n = 101) or NB1 (n...
Source: Cell Transplantation - October 8, 2009 Category: Cytology Authors: Brandhorst H, Friberg A, Nilsson B, Andersson HH, Felldin M, Foss A, Salmela K, Tibell A, Tufveson G, Korsgren O, Brandhorst D Tags: Cell Transplant Source Type: journals

Quantitative Assessment of Islet Cell Products: Estimating the Accuracy of the Existing Protocol and Accounting for Islet Size Distribution.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The ability to consistently and reliably assess the total number and the size distribution of isolated pancreatic islet cells from a small sample is of crucial relevance for the adequate characterization of islet cell preparations used for research or transplantation purposes. Here, data from a large number of isolations were used to establish a continuous probability density function describing the size distribution of human pancreatic islets. This function was then used to generate a polymeric microsphere mixture with a composition resembling those of isolated islets, which, in turn, was used to quantitatively assess...
Source: Cell Transplantation - October 8, 2009 Category: Cytology Authors: Buchwald P, Wang X, Khan A, Bernal A, Fraker C, Inverardi L, Ricordi C Tags: Cell Transplant Source Type: journals

Reversal of dopaminergic degeneration in a parkinsonian rat following micrografting of human bone marrow-derived neural progenitors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Parkinson's disease (PD) is a common neurodegenerative disease characterized by theselective loss of dopaminergic (DA) neurons in the midbrain. Various types of stem cells that have potential to differentiate into DA neurons are being investigated as cellular therapies for PD. Stem cells also secrete growth factors and therefore may also have therapeutic effects in promoting the health of diseased DA neurons in the PD brain. To address this possibility in an experimental model of PD, bone marrow-derived neuroprogenitor-like cells were generated from bone marrow procured from healthy human adult volunteers and their pot...
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: Glavaski-Joksimovic A, Virag T, Chang QA, West NC, Mangatu TA, McGrogan MP, Dugich-Djordjevic M, Bohn MC Tags: Cell Transplant Source Type: journals

Liver cell transplantation: Basic investigations for safe application in infants and small children.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Liver cell transplantation (LCT) is a very promising method for the use in pediatric patients. It is significantly less invasive than whole organ transplantation, but has the potential to cure or at least to substantially improve severe disorders like inborn errors of metabolism or acute liver failure. Prior to a widespread use of the technique in children, some important issues regarding safety and efficacy must be addressed. We developed a mathematical model to estimate total hepatocyte counts in relation to bodyweight to make possible more appropriate dose calculations. Different liver cell suspensions were studied ...
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: Meyburg J, Alexandrova K, Barthold M, Kafert-Kasting S, Schneider AS, Attaran M, Hoerster F, Schmidt J, Hoffmann GF, Ott M Tags: Cell Transplant Source Type: journals

2007 Update on Allogeneic Islet Transplantation from the Collaborative Islet Transplant Registry (CITR).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
As of October 1, 2007, 25 North American medical institutions and one European islet transplant center reported detailed information to the Registry on 315 allograft recipients; of which 285 were islet alone (IA) and 30 were islet after kidney. Of the 114 IA recipients expected at four years after their last infusion, 12% were insulin independent, 16% were insulin dependent with detectable C-peptide, 40% had no detectable C-peptide, and 32% had missing C-peptide data or were lost to follow-up. Of the IA recipients, 72% achieved insulin independence at least once over three years and multiple infusions. Factors associat...
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: Group TC Tags: Cell Transplant Source Type: journals

Cell-cell interaction promotes rat marrow stromal cell differentiation into endothelial cell via activation of TACE/TNF-alpha signaling.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We demonstrated for the first time that direct MSCs-rBMECs interaction stimulated the endothelial differentiation of MSCs via TACE/TNF alpha signaling. PMID: 19796498 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: Xu J, Liu X, Chen J, Zacharek A, Cui X, Savant-Bhonsale S, Chopp M, Liu Z Tags: Cell Transplant Source Type: journals

Who's in favor of translational cell therapy for stroke: STEPS forward please?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A consortium of translational stem cell and stroke experts from multiple academic institutes and biotechnology companies, under the guidance of the government (FDA/NIH), is missing. Here, we build a case for the establishment of this consortium if cell therapy for stroke is to advance from the laboratory to the clinic. PMID: 19796499 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: Chopp M, Steinberg GK, Kondziolka D, Lu M, Bliss TM, Li Y, Hess DC, Borlongan CV Tags: Cell Transplant Source Type: journals

STEPS Toward the Right Direction.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cell therapy for stroke may benefit from translational research guidelines to aid its entry into the clinic as a safe and effective treatment. PMID: 19796500 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: Sladek JR, Sanberg PR Tags: Cell Transplant Source Type: journals

Tnf, pig cd86 and vcam-1 identified as potential targets for intervention in xenotransplantation of pig chondrocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
TNF, PIG CD86 AND VCAM-1 IDENTIFIED AS POTENTIAL TARGETS FOR INTERVENTION IN XENOTRANSPLANTATION OF PIG CHONDROCYTES. Cell Transplant. 2009 Sep 28; Authors: Sommaggio R, Máñez R, Costa C Xenotransplantation of genetically engineered porcine chondrocytes may benefit many patients who suffer cartilage defects. In this work, we sought to elucidate the molecular bases of the cellular response to xenogeneic cartilage. To this end, we isolated pig costal chondrocytes (PCC) and conducted a series of functional studies. First, we determined by flow cytometry the cell surface expression of multiple immunoregul...
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: Sommaggio R, Máñez R, Costa C Tags: Cell Transplant Source Type: journals

Functional characterization of hepatocytes for cell transplantation: customized cell preparation for each receptor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The first indication of hepatocyte transplantation is inborn liver-based metabolic disorders and among which, urea cycle disorders leading to the impairment to detoxify ammonia and Crigler-Najjar Syndrome type I, a deficiency in the hepatic UDP-glucuronosyltransferase 1A1, present the highest incidence. Metabolically qualified human hepatocytes are required for clinical infusion. We proposed fast and sensitive procedures to determine their suitability for transplantation. For this purpose, viability, attachment efficiency and metabolic functionality (ureogenic capability, cytochrome P450 and phase II activities) are as...
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: A BC, Mt D, A L, E P, J M, Jv C, Mj GL Tags: Cell Transplant Source Type: journals

Functional recovery after the transplantation of neurally differentiated mesenchymal stem cells derived from bone marrow in a rat model of spinal cord injury.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to investigate functional recovery after the transplantation of mesenchymal stem cells (MSCs) or neurally differentiated MSCs (NMSCs) derived from bone marrow in a rat model of spinal cord injury (SCI). Sprague-Dawley rats were subjected to incomplete SCI using an NYU impactor to create a free drop contusion at the T9 level. The SCI rats were then classified into three groups; MSCs, NMSCs and phosphated buffered saline (PBS)-treated groups. The cells or PBS were administrated 1 week after SCI. Basso-Beattie-Bresnahan (BBB) locomotor rating scores were measured at 1-week intervals for 9 weeks. Somato...
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: Cho SR, Kim YR, Kang HS, Yim SH, Park CI, Min YH, Lee BH, Shin JC, Lim JB Tags: Cell Transplant Source Type: journals

Laser Capture Microdissection as a new tool to assess graft infiltrating lymphocytes gene profile in islet transplantation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Innovative tolerogenic protocols in transplantation would take advantage of the development of new tools capable of evaluating the impact of these treatments on immune system. These assays have potential for clinical application. Currently, many of these studies are based on the analysis of peripheral lymph nodes and blood-derived cells, where the percentage of alloantigen-specific cells can be low or even unpredictable. We combined a Laser Capture Microdissection (LCM) technique with Real-Time PCR (RT-PCR) to evaluate gene profile of islet infiltrating lymphocytes. Donor Lewis rats islets were transplanted under the k...
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: Vergani A, Clissi B, Sanvito F, Doglioni C, Fiorina P, Pardi R Tags: Cell Transplant Source Type: journals

Crosslinking enhances deposition of human endothelial progenitor cells in the rat heart after intracoronary transplantation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Transplantation of human endothelial progenitor cells (EPCs) may improve vascularization and left ventricular function after myocardial infarction. The scope of this study was to explore, whether crosslinking of EPCs may enhance the deposition of cells in the rat heart after clinical-like, intracoronary transplantation. To this end, (1)(1)(1)Indium-oxinate labelled human EPCs were infused by a minimally invasive technique into the coronary arteries of immuno-suppressed Wistar rats under control conditions and after ischemia-reperfusion. In a second set of experiments hEPCs were treated with phytohemagglutinin to create...
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: Burghoff S, Ding Z, Blaszczyk A, Wirrwar A, Buchholz D, Müller HW, Schrader J Tags: Cell Transplant Source Type: journals

Increased Apelin Following Bone Marrow Mononuclear Cell Transplantation Contributes to the Improvement of Cardiac Function in Patients with Severe Heart Failure.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We previously reported that intracoronary implantation of bone marrow mononuclear cells (BMMC) into ischemic hearts improved cardiac function after myocardial infarction. However, the mechanisms have not been elucidated. The present study investigates whether apelin, a newly described inotropic peptide with important cardiovascular regulatory properties, contributes to the functional improvement in patients with severe heart failure after cell transplantation. Forty consecutive patients with severe heart failure secondary to myocardial infarction were assigned to the BMMC therapy group or the standard medication group ...
Source: Cell Transplantation - September 27, 2009 Category: Cytology Authors: Gao LR, Xu RY, Zhang NK, Chen Y, Wang ZG, Zhu ZM, Fei YX, Cao Y, Xu HT, Yang AY Tags: Cell Transplant Source Type: journals

Spreading the wings of organ biology further.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19775507 [PubMed - in process] (Source: Cell Transplantation)
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Kobayashi N Tags: Cell Transplant Source Type: journals

Regenerative medicine for diabetes mellitus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In diabetes, a loss of pancreatic beta-cells causes insulin dependency. When insulin dependency is caused by type 1 diabetes or pancreatic diabetes, for example, pancreatic beta-cells need to be regenerated for definitive treatment. The methods for generating pancreatic beta-cells include a method of creating pancreatic beta-cells in vitro and implanting them into the body and a method of regenerating pancreatic beta-cells in the body via gene introduction or the administration of differential proliferation factors to the body. Moreover, the number of pancreatic beta-cells is also low in type 2 diabetes, caused by the ...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Kobayashi N, Yuasa T, Okitsu T Tags: Cell Transplant Source Type: journals

Pancreas preservation by the two-layer method: does it have a beneficial effect compared with simple preservation in University of Wisconsin solution?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A large number of reports have shown that the two-layer method (TLM), which employs oxygenated perfluorochemical (PFC) and University of Wisconsin (UW) solution, is superior to simple cold storage in UW in islet transplantation. However, two recent large-scale studies showed no beneficial effect of TLM compared with UW storage in human islet transplantation. We reevaluated the effect of TLM by following three groups: group 1: UW simple storage; group 2: TLM performed by multiorgan procurement teams (not specialists of islet isolation); and group 3: TLM performed by specialists of islet isolation (Noguchi and Matsumoto)...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Noguchi H, Levy MF, Kobayashi N, Matsumoto S Tags: Cell Transplant Source Type: journals

Beneficial storage effects of epigallocatechin-3-o-gallate on the articular cartilage of rabbit osteochondral allografts.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A fresh osteochondral allograft is one of the most effective treatments for cartilage defects of the knee. Despite the clinical success, fresh osteochondral allografts have great limitations in relation to the short storage time that cartilage tissues can be well-preserved. Fresh osteochondral grafts are generally stored in culture medium at 4 degrees C. While the viability of articular cartilage stored in culture medium is significantly diminished within 1 week, appropriate serology testing to minimize the chances for the disease transmission requires a minimum of 2 weeks. (-)-Epigallocatechin-3-O-gallate (EGCG) has d...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Bae JY, Matsumura K, Wakitani S, Kawaguchi A, Tsutsumi S, Hyon SH Tags: Cell Transplant Source Type: journals

Long-term preservation of rat skin tissue by epigallocatechin-3-o-gallate.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study examined the effects of EGCG on skin cryopreservation. Skin sample biopsy specimens from GFP rats were previously treated with/without EGCG then moved to -196 degrees C. Skin samples were transplanted to nude mice after 2, 8, and 24 weeks of preservation. Glucose consumption was measured after thawing to assess the metabolic activity. Two weeks later the transplanted skin grafts were excised and histologically analyzed. Histological examinations revealed the degeneration of the epidermal and dermal layers in all groups. In the EGCG groups, the grafts showed higher integrity in the epidermal layer and dermal matr...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Kim H, Kawazoe T, Matsumura K, Suzuki S, Hyon SH Tags: Cell Transplant Source Type: journals

Preservation of platelets by adding epigallocatechin-3-o-gallate to platelet concentrates.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The effect of epigallocatechin-3-O-gallate (EGCG), a major component of green tea, on platelet preservation was evaluated. Single donor platelets (N = 10) were collected and preserved by the standard method. EGCG was added to the platelet concentrates before preservation and then the functional and biochemical parameters were monitored throughout the storage period. After 6 days of preservation, the aggregability of the platelets was significantly maintained by addition of 50 and 100 microg/ml of EGCG. Platelet prothrombinase activity was also significantly retained by the addition of EGCG. The accumulation of P-select...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Matsumura K, Takayama H, Bae JY, Kurihara M, Tsutsumi S, Hyon SH Tags: Cell Transplant Source Type: journals

A study on the perfusion preservation, resuscitation, and transplantation of a rat heart isolated for 96 hours.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Krebs-Henseleit (KH) solution was used to fill the heart chamber of an isolated rat heart before it was immersed in perfluorocarbon (PFC), which is an inert fluid. A gas mixture (PCO(2) = 150 hPa and PO(2) = 850 hPa) was then aerated at a constant rate into the PFC solution, and the isolated heart was thereafter preserved for 96 h with KH solution perfused continuously at a rate of 0.1 ml/h from the aorta of the isolated heart through a cannula. After preservation, the preserved heart was heterotopically transplanted into the neck of a recipient rat and then it was resuscitated. Using this method for preserving mammali...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Hatayama N, Yoshida Y, Seki K Tags: Cell Transplant Source Type: journals

Study on the preservation with CO (PCO = 200-2,000 hPa), resuscitation, and heterotopic transplantation of an isolated rat heart.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This report demonstrates that significant reproducibility has been verified. The heart was removed from the refrigerator 24 h later and heterotopic heart transplantation was performed in the right neck of a recipient rat and the pulsating of the transplanted heart was detected by an electrocardiogram. PMID: 19775514 [PubMed - in process] (Source: Cell Transplantation)
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Yoshida Y, Hatayama N, Seki K Tags: Cell Transplant Source Type: journals

Comparison of trypsin inhibitors in preservation solution for islet isolation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we compared ulinastatin with another trypsin inhibitor, Pefabloc, in preservation solution for islet isolation. Islet yield before purification was higher in the MK/PFC group compared with the ET-Kyoto with Pefabloc (PK)/PFC group. The stimulation index was higher for the MK/PFC group than for the PK/PFC group. These data suggest that ET-Kyoto with ulinastatin was the better combination for pancreas preservation than ET-Kyoto with Pefabloc. Based on these data, we now use ET-Kyoto solution with ulinastatin for clinical islet transplantation. PMID: 19775515 [PubMed - in process] (Source: Cell Transplantation)
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Noguchi H, Ueda M, Hayashi S, Kobayashi N, Okitsu T, Iwanaga Y, Nagata H, Liu X, Kamiya H, Levy MF, Matsumoto S Tags: Cell Transplant Source Type: journals

Estimation of donor usability for islet transplantation in the United States with the kyoto islet isolation method.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we applied the KIIM for brain-dead donors and reevaluated donor usability based on the Japanese islet donor criteria. Islets were isolated with the Ricordi method using pancreata stored in University of Wisconsin (UW) solution (UW group) or by the two-layer method (TLM group) or the TLM combined with ductal injection (DI group). We implemented the KIIM (KIIM group) to confirm the effect of the KIIM on brain-dead donors. Donor charts in Texas from 2005 to 2006 were reviewed. If pancreata were not used clinically, the reason was reviewed and donors were reevaluated based on Japanese criteria. There were no sig...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Matsumoto S, Noguchi H, Hatanaka N, Shimoda M, Kobayashi N, Jackson A, Onaca N, Naziruddin B, Levy MF Tags: Cell Transplant Source Type: journals

SUITO index for evaluation of efficacy of single donor islet transplantation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we evaluated the SUITO index for the prediction of clinical outcome after single islet transplantation. Single islet transplantations were performed into six type 1 diabetic patients. Isolated islets were quantitatively assessed at the time of transplantation. The SUITO index was calculated as follows: fasting C-peptide (ng/dl)/[fasting blood glucose (mg/dl) - 63] x 1500. Islet yield/recipient's body weight and SUITO index were evaluated, along with HbA(1C), relative insulin dose (insulin dose posttransplant/pretransplant), and M-values. HbA(1C) improved in all cases, irrespective of the SUITO index score or...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Matsumoto S, Noguchi H, Hatanaka N, Shimoda M, Kobayashi N, Jackson A, Onaca N, Naziruddin B, Levy MF Tags: Cell Transplant Source Type: journals

Establishment of mouse pancreatic stem cell line.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we established a mouse pancreatic stem cell line without genetic manipulation. The duct-rich population after islet isolation was inoculated into 96-well plates in limiting dilution. From over 200 clones, 15 clones were able to be cultured for over 3 months. The HN#13 cells, which had the highest expression of insulin mRNA after induction, expressed PDX-1 transcription factor, glucagon-like peptide-1 (GLP-1) receptor, and cytokeratin-19 (duct-like cells). These cells continue to divide actively beyond the population doubling level (PDL) of 300. Exendin-4 treatment and transduction of PDX-1 and NeuroD protein...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Noguchi H, Oishi K, Ueda M, Yukawa H, Hayashi S, Kobayashi N, Levy MF, Matusmoto S Tags: Cell Transplant Source Type: journals

Recombinant sendai virus-mediated gene transfer to mouse pancreatic stem cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we investigated the in vitro gene transfer efficiency of Sendai virus (SeV) vectors, a paramyxovirus vector that can efficiently introduce foreign genes without toxicity into several cell types, including pancreatic stem cells. The dose-dependent GFP expression of pancreatic stem cells transfected with SeV vectors after 48 h of culture at 37 degrees C was observed. The transfection of pancreatic stem cells with SeV vectors and AdV vectors results in equal expression of the transgene (GFP expression) in the cells after 48 h of culture at 37 degrees C. Although the transfection of pancreatic stem cells with Ad...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Oishi K, Noguchi H, Yukawa H, Inoue M, Takagi S, Iwata H, Hasegawa M, Hayashi S Tags: Cell Transplant Source Type: journals

Differential ability of somatic stem cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we investigated the transdifferentiation potential of somatic stem cells using adipose tissue-derived stem/progenitor cells (ASCs; mesodermal stem cells) and pancreatic stem cells (endodermal stem cells). Previous reports from other groups describe the protocol that has been used to differentiate ASCs or mesenchymal stem cells (MSCs) in bone marrow into insulin-producing cells. Induction 1: ASCs were cultured for 3 days in ultra-low attachment plates under serum-free conditions. Induction 2: ASCs were cultured for 24 h with L-DMEM, and reinduced with serum-free H-DMEM for another 10 h. Unlike previous report...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Oishi K, Noguchi H, Yukawa H, Hayashi S Tags: Cell Transplant Source Type: journals

Quantum dots for labeling adipose tissue-derived stem cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, negatively charged QDs655 were applied for ASCs labeling, with the cationic liposome, Lipofectamine. The cytotoxicity of QDs655-Lipofectamine was assessed for ASCs. Although some cytotoxicity was observed in ASCs transfected with more than 2.0 nM of QDs655, none was observed with less than 0.8 nM. To evaluate the time dependency, the fluorescent intensity with QDs655 was observed until 24 h after transfection. The fluorescent intensity gradually increased until 2 h at the concentrations of 0.2 and 0.4 nM, while the intensity increased until 4 h at 0.8 nM. The ASCs were differentiated into both adipogenic and...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Yukawa H, Mizufune S, Mamori C, Kagami Y, Oishi K, Kaji N, Okamoto Y, Takeshi M, Noguchi H, Baba Y, Hamaguchi M, Hamajima N, Hayashi S Tags: Cell Transplant Source Type: journals

Comparison of sendai virus-mediated gene transfer efficiency to adhesive and floating adipose tissue-derived stem cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study assessed the gene transfer efficiency to floating ASCs with SeV vectors. Although a slight cytotoxicity was observed, the efficiency of gene transfer to cells in the floating state was much higher at all times and all concentrations at MOIs of 2, 10, and 20 than in the adhesion state. Moreover, ASCs transfected with SeV vectors in floating state have the same potential for their differentiation into specific tissues, such as adipocytes and osteocytes, as untransfected ASCs. These data suggest that SeV transfection to ASCs in the floating state could therefore be useful for gene transfer technology. PMID: 197...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Yukawa H, Noguchi H, Oishi K, Inoue M, Hasegawa M, Hamaguchi M, Hamajima N, Hayashi S Tags: Cell Transplant Source Type: journals

Cell transplantation of adipose tissue-derived stem cells in combination with heparin attenuated acute liver failure in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, the transplantation of ASCs in combination with heparin was thus found to effectively treat acute liver failure. PMID: 19775523 [PubMed - in process] (Source: Cell Transplantation)
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Yukawa H, Noguchi H, Oishi K, Takagi S, Hamaguchi M, Hamajima N, Hayashi S Tags: Cell Transplant Source Type: journals

Cryopreservation in situ of cell monolayers on collagen vitrigel membrane culture substrata: ready-to-use preparation of primary hepatocytes and ES cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cryopreservation is generally performed on cells in suspension. In the case of adherent cells such as hepatocytes, a loss of their ability to attach is a more serious problem than a decreased viability after cryopreservation. We herein report a novel technology of direct in situ cryopreservation of cells cultured on collagen vitrigel membranes, which have excellent mechanical strength and can be easily handled by tweezers even when coated with cultured cells. Rat primary hepatocytes, mitomycin C-treated mouse fibroblasts (feeder cells for ES cells), and mouse ES cells on the feeder cells were cultured on collagen vitri...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Miyamoto Y, Enosawa S, Takeuchi T, Takezawa T Tags: Cell Transplant Source Type: journals

Superagonist CD28 antibody preferentially expanded Foxp3-expressing nTreg cells and prevented graft-versus-host diseases.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Regulatory lymphocytes play a pivotal role in preventing organ-specific autoimmune disease and in induction and maintenance of tolerance in various experimental transplantation models. The enhancement of the number and activity of peripheral CD4(+)CD25(+) Treg cells is an obvious goal for the treatment of autoimmunity and for the suppression of alloreactions. The present study demonstrates that naturally occurring CD4(+)CD25(+) Treg (nTreg) cells preferentially proliferate to a fourfold increase within 3 days in response to the administration of a single superagonistic CD28-specific monoclonal antibody (supCD28 mAb). T...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Kitazawa Y, Fujino M, Li XK, Xie L, Ichimaru N, Okumi M, Nonomura N, Tsujimura A, Isaka Y, Kimura H, Hünig T, Takahara S Tags: Cell Transplant Source Type: journals

Comparative study of the cellular pharmacodynamics of calcineurin inhibitors between patients with chronic renal failure awaiting renal transplantation and cirrhosis patients awaiting liver transplantation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The in vitro response of peripheral blood mononuclear cells (PBMCs) to the suppressive effects of calcineurin inhibitors is known to correlate with the clinical efficacy of drugs used in renal transplantations. The present study was conducted to examine the differences of PBMC responses to calcineurin inhibitors between chronic renal failure (CRF) patients awaiting renal transplantation and cirrhosis patients awaiting liver transplantation. The study included 99 CRF patients awaiting renal transplantation and 27 cirrhosis patients awaiting liver transplantation. Twenty milliliters of venous blood was taken 1-7 days bef...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Kihara Y, Matsuno N, Mijiti A, Nagao T, Takeuchi H, Unezaki S, Hirano T Tags: Cell Transplant Source Type: journals

Global expression profiles in 1-hour biopsy specimens of human kidney transplantation from donors after cardiac death.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Because of the worldwide shortage of renal grafts, kidney transplantation (KTx) from donors after cardiac death (DCD) is an alternative way to obtain KTx from brain-dead donors. Although the prognosis of DCD KTx is gradually improving, the graft often undergoes delayed graft function (DGF), rendering the control of DGF essential for post-KTx patient care. In an attempt to characterize etiology of DGF, genome-wide gene expression profiling was performed using renal biopsy samples performed at 1 h after KTx from DCD and the data were compared with those of KTx from living donors (LD). A total of 526 genes were differenti...
Source: Cell Transplantation - September 25, 2009 Category: Cytology Authors: Kusaka M, Kuroyanagi Y, Mori T, Nagaoka K, Sasaki H, Maruyama T, Hayakawa K, Shiroki R, Kurahashi H, Hoshinaga K Tags: Cell Transplant Source Type: journals