Gadolinium retention effect on macrophages — a potential cause of MRI contrast agent Dotarem toxicity
AbstractGadolinium is a component of the MRI contrast agent Dotarem. Although Dotarem is the least toxic among MRI contrasts used, gadolinium present in Dotarem accumulates for many years in various organs and tissues exerting toxic effects. We showed previously that gadolinium remains in macrophages for at least 7 days after exposure to Dotarem. However, very little is known about the effect of gadolinium retention on the immune cells such as macrophages. We studied the effect of 1-day and 7-day retention of gadolinium on various functions and molecular pathways of macrophages. Gadolinium retention for 7 days decrease...
Source: Cell and Tissue Research - April 16, 2024 Category: Cytology Source Type: research
CD34+ synovial fibroblasts exhibit high osteogenic potential in synovial chondromatosis
This study aimed to identify the abnormally differentiated progenitor cells and possible pathogenic signaling pathways. Loose bodies and synovium were obtained from patients with SC during knee arthroplasty. Single-cell RNA sequencing was used to identify cell subsets and their gene signatures in SC synovium. Cells derived from osteoarthritis (OA) synovium were used as controls. Multi-differentiation and colony-forming assays were used to identify progenitor cells. The roles of transcription factors and signaling pathways were investigated through computational analysis and experimental verification. We identified an incre...
Source: Cell and Tissue Research - April 11, 2024 Category: Cytology Source Type: research
Differential expression of neuropeptide F in the digestive organs of female freshwater prawn, Macrobrachium rosenbergii, during the ovarian cycle
AbstractNeuropeptide F is a key hormone that controls feeding in invertebrates, including decapod crustaceans. We investigated the differential expression ofMacrobrachium rosenbergii neuropeptide F (MrNPF) in the digestive organs of female prawns,M. rosenbergii, during the ovarian cycle. By using RT-qPCR, the expression of MrNPF mRNA in the esophagus (ESO), cardia (CD), and pylorus (PY) of the foregut (FG) gradually increased from stage II and peaked at stage III. In the midgut (MG), hindgut (HG), and hepatopancreas (HP), MrNPF mRNA increased from stage I, reaching a maximal level at stage II, and declined by about half at...
Source: Cell and Tissue Research - April 9, 2024 Category: Cytology Source Type: research
Involvement of ANO1 currents in pacemaking of PDGFR α-positive specialised smooth muscle cells in rat caudal epididymis
AbstractThe epididymal duct exhibits spontaneous phasic contractions (SPCs) to store and transport sperm. Here, we explored molecular identification of pacemaker cells driving SPCs in the caudal epididymal duct and also investigated properties of pacemaker currents underlying SPCs focusing on ANO1 Ca2+-activated Cl− channels (CaCCs). Immunohistochemistry was performed to visualise the distribution of platelet-derived growth factor receptor α (PDGFRα)- or ANO1-positive cells in the rat caudal epididymal duct. Perforated whole-cell patch clamp technique was applied to enzymatically isolated epididymal cells, while SPCs w...
Source: Cell and Tissue Research - April 8, 2024 Category: Cytology Source Type: research
Correction to: Induction of forkhead box M1 (FoxM1) by EGF through ERK signaling pathway promotes trophoblast cell invasion
(Source: Cell and Tissue Research)
Source: Cell and Tissue Research - April 5, 2024 Category: Cytology Source Type: research
Blood-testis barrier: a review on regulators in maintaining cell junction integrity between Sertoli cells
AbstractThe blood-testis barrier (BTB) is formed adjacent to the seminiferous basement membrane. It is a distinct ultrastructure, partitioning testicular seminiferous epithelium into apical (adluminal) and basal compartments. It plays a vital role in developing and maturing spermatocytes into spermatozoa via reorganizing its structure. This enables the transportation of preleptotene spermatocytes across the BTB, from basal to adluminal compartments in the seminiferous tubules. Several bioactive peptides and biomolecules secreted by testicular cells regulate the BTB function and support spermatogenesis. These peptides activ...
Source: Cell and Tissue Research - April 2, 2024 Category: Cytology Source Type: research
Telocytes: current methods of research, challenges and future perspectives
AbstractTelocytes (TCs) are CD34-positive interstitial cells that have long cytoplasmic projections, called telopodes; they have been identified in several organs and in various species. These cells establish a complex communication network between different stromal and epithelial cell types, and there is growing evidence that they play a key role in physiology and pathology. In many tissues, TC network impairment has been implicated in the onset and progression of pathological conditions, which makes the study of TCs of great interest for the development of novel therapies. In this review, we summarise the main methods in...
Source: Cell and Tissue Research - March 28, 2024 Category: Cytology Source Type: research
Correction to: Nescient helix-loop-helix 1 (Nhlh1) is a novel activating transcription factor 5 (ATF5) target gene in olfactory and vomeronasal sensory neurons in mice
(Source: Cell and Tissue Research)
Source: Cell and Tissue Research - March 26, 2024 Category: Cytology Source Type: research
Challenges of mesenchymal stem cells in the clinical treatment of COVID-19
AbstractThe 2019 coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has brought an enormous public health burden to the global society. The duration of the epidemic, the number of infected people, and the widespread of the epidemic are extremely rare in modern society. In the initial stage of infection, people generally show fever, cough, and dyspnea, which can lead to pneumonia, acute respiratory syndrome, kidney failure, and even death in severe cases. The strong infectivity and pathogenicity of SARS-CoV-2 make it more urgent to find an effective treatment. Mesenchymal s...
Source: Cell and Tissue Research - March 21, 2024 Category: Cytology Source Type: research
Joubert syndrome-derived induced pluripotent stem cells show altered neuronal differentiation in vitro
AbstractJoubert syndrome (JS) is a recessively inherited congenital ataxia characterized by hypotonia, psychomotor delay, abnormal ocular movements, intellectual disability, and a peculiar cerebellar and brainstem malformation, the “molar tooth sign.” Over 40 causative genes have been reported, all encoding for proteins implicated in the structure or functioning of the primary cilium, a subcellular organelle widely present in embryonic and adult tissues. In this paper, we developed anin vitro neuronal differentiation model using patient-derived induced pluripotent stem cells (iPSCs), to evaluate possible neurodevelopme...
Source: Cell and Tissue Research - March 19, 2024 Category: Cytology Source Type: research
CoQ10 targeted hippocampal ferroptosis in a status epilepticus rat model
AbstractStatus epilepticus (SE), the most severe form of epilepsy, leads to brain damage. Uncertainty persists about the mechanisms that lead to the pathophysiology of epilepsy and the death of neurons. Overloading of intracellular iron ions has recently been identified as the cause of a newly recognized form of controlled cell death called ferroptosis. Inhibiting ferroptosis has shown promise as a treatment for epilepsy, according to recent studies. So, the current study aimed to assess the possible antiepileptic impact of CoQ10 either alone or with the standard antiepileptic drug sodium valproate (SVP) and to evaluate th...
Source: Cell and Tissue Research - March 19, 2024 Category: Cytology Source Type: research
Correction to: BMP2 induces chondrogenic differentiation, osteogenic differentiation and endochondral ossification in stem cells
(Source: Cell and Tissue Research)
Source: Cell and Tissue Research - March 19, 2024 Category: Cytology Source Type: research
Characteristics of A-type voltage-gated K+ currents expressed on sour-sensing type III taste receptor cells in mice
AbstractSour taste is detected by type III taste receptor cells that generate membrane depolarization with action potentials in response to HCl applied to the apical membranes. The shape of action potentials in type III cells exhibits larger afterhyperpolarization due to activation of transient A-type voltage-gated K+ currents. Although action potentials play an important role in neurotransmitter release, the electrophysiological features of A-type K+ currents in taste buds remain unclear. Here, we examined the electrophysiological properties of A-type K+ currents in mouse fungiform taste bud cells using in-situ whole-cell...
Source: Cell and Tissue Research - March 16, 2024 Category: Cytology Source Type: research
The role of vitamin D receptor in predentin mineralization and dental repair after injury
AbstractDentin is a permeable and complex tubular composite formed by the mineralization of predentin that mineralization and repair are of considerable clinical interest during dentin homeostasis. The role ofVdr, a receptor of vitamin D, in dentin homeostasis remains unexplored. The aim of the present study was to assess the impact ofVdr on predentin mineralization and dental repair.Vdr-knockout (Vdr−/−) mice models were constructed; histology and immunohistochemistry analyses were conducted for bothWT andVdr−/− mice. The finding revealed a thicker predentin inVdr−/− mice, characterized by higher expression of...
Source: Cell and Tissue Research - March 16, 2024 Category: Cytology Source Type: research
Regional differences in the ultrastructure of mucosal macrophages in the rat large intestine
In conclusion, the present pilot study suggested that the quantity of some organelles (lysosomes and vacuoles) in macrophages differed between the cecum and the descending colon and that there were some region-specific subsets of macrophages like nerve-associated macrophages in the cecum. (Source: Cell and Tissue Research)
Source: Cell and Tissue Research - March 15, 2024 Category: Cytology Source Type: research
