The presence of CpGs in AAV gene therapy vectors induces a plasmacytoid dendritic cell-like population very early after administration
Cell Immunol. 2024 Mar 19;399-400:104823. doi: 10.1016/j.cellimm.2024.104823. Online ahead of print.ABSTRACTAAV-mediated gene transfer is a promising platform still plagued by potential host-derived, antagonistic immune responses to therapeutic components. CpG-mediated TLR9 stimulation activates innate immune cells and leads to cognate T cell activation and suppression of transgene expression. Here, we demonstrate that CpG depletion increased expression of an antibody transgene product by 2-3-fold as early as 24 h post-vector administration in mice. No significant differences were noted in anti-transgene product/ anti-AAV ...
Source: Cellular Immunology - March 23, 2024 Category: Allergy & Immunology Authors: Justin D Glenn Henos Negash William Henry Randolph Qian Ye Liu Olivier Danos Joseph T Bruder Subha Karumuthil-Melethil Source Type: research

The presence of CpGs in AAV gene therapy vectors induces a plasmacytoid dendritic cell-like population very early after administration
Cell Immunol. 2024 Mar 19;399-400:104823. doi: 10.1016/j.cellimm.2024.104823. Online ahead of print.ABSTRACTAAV-mediated gene transfer is a promising platform still plagued by potential host-derived, antagonistic immune responses to therapeutic components. CpG-mediated TLR9 stimulation activates innate immune cells and leads to cognate T cell activation and suppression of transgene expression. Here, we demonstrate that CpG depletion increased expression of an antibody transgene product by 2-3-fold as early as 24 h post-vector administration in mice. No significant differences were noted in anti-transgene product/ anti-AAV ...
Source: Cellular Immunology - March 23, 2024 Category: Allergy & Immunology Authors: Justin D Glenn Henos Negash William Henry Randolph Qian Ye Liu Olivier Danos Joseph T Bruder Subha Karumuthil-Melethil Source Type: research

The presence of CpGs in AAV gene therapy vectors induces a plasmacytoid dendritic cell-like population very early after administration
Cell Immunol. 2024 Mar 19;399-400:104823. doi: 10.1016/j.cellimm.2024.104823. Online ahead of print.ABSTRACTAAV-mediated gene transfer is a promising platform still plagued by potential host-derived, antagonistic immune responses to therapeutic components. CpG-mediated TLR9 stimulation activates innate immune cells and leads to cognate T cell activation and suppression of transgene expression. Here, we demonstrate that CpG depletion increased expression of an antibody transgene product by 2-3-fold as early as 24 h post-vector administration in mice. No significant differences were noted in anti-transgene product/ anti-AAV ...
Source: Cellular Immunology - March 23, 2024 Category: Allergy & Immunology Authors: Justin D Glenn Henos Negash William Henry Randolph Qian Ye Liu Olivier Danos Joseph T Bruder Subha Karumuthil-Melethil Source Type: research

Helicobacter pylori secretary Proteins-Induced oxidative stress and its role in NLRP3 inflammasome activation
This study evaluates the role of H. pylori secreted concentrated proteins (HPSCP) related oxidative stress role in NLRP3 inflammasome activation and macrophage differentiation. To perceive the role of ROS/RNS, THP-1 and AGS cells were treated with 10 μM diphenyleneiodonium (DPI), 50 μM salicyl hydroxamic acid (SHX), 5 μM Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (FCCP), which are specific inhibitors of NADPH oxidase (NOX), Myeloperoxidase (MPO), and mitochondrial oxidative phosphorylation respectively. Cells were also treated with 10 μM of NOS2 inhibitor l-NMMA and 10 μM of N-acetyl cysteine (NAC), a free ...
Source: Cellular Immunology - March 22, 2024 Category: Allergy & Immunology Authors: Sandeep Kumar Monisha Dhiman Source Type: research

Helicobacter pylori secretary Proteins-Induced oxidative stress and its role in NLRP3 inflammasome activation
This study evaluates the role of H. pylori secreted concentrated proteins (HPSCP) related oxidative stress role in NLRP3 inflammasome activation and macrophage differentiation. To perceive the role of ROS/RNS, THP-1 and AGS cells were treated with 10 μM diphenyleneiodonium (DPI), 50 μM salicyl hydroxamic acid (SHX), 5 μM Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (FCCP), which are specific inhibitors of NADPH oxidase (NOX), Myeloperoxidase (MPO), and mitochondrial oxidative phosphorylation respectively. Cells were also treated with 10 μM of NOS2 inhibitor l-NMMA and 10 μM of N-acetyl cysteine (NAC), a free ...
Source: Cellular Immunology - March 22, 2024 Category: Allergy & Immunology Authors: Sandeep Kumar Monisha Dhiman Source Type: research

HLA-class-I expression loss, tumor microenvironment and breast cancer prognosis
We examined the expression patterns of HLA-class-I molecules in a series of 175 patients with operable breast cancer (BCa). Extensive loss of BCa cell HLA-class-I expression was noted 76.6 % of patients (27.5 % complete loss). A significant association of HLA-preservation with high TIL-density (p = 0.001) was documented. Preservation of HLA was evident only in BCa carcinomas with low HIF1α expression and high TIL-density. Cell line experiments (MCF7 and T47D) showed that induction of HLAs in cancer cells following incubation with lymphocytes or IFNγ, was abrogated under hypoxic conditions. HLA-preservation was linked wit...
Source: Cellular Immunology - March 20, 2024 Category: Allergy & Immunology Authors: Alexandra Giatromanolaki Georgios D Michos Erasmia Xanthopoulou Michael I Koukourakis Source Type: research

HLA-class-I expression loss, tumor microenvironment and breast cancer prognosis
We examined the expression patterns of HLA-class-I molecules in a series of 175 patients with operable breast cancer (BCa). Extensive loss of BCa cell HLA-class-I expression was noted 76.6 % of patients (27.5 % complete loss). A significant association of HLA-preservation with high TIL-density (p = 0.001) was documented. Preservation of HLA was evident only in BCa carcinomas with low HIF1α expression and high TIL-density. Cell line experiments (MCF7 and T47D) showed that induction of HLAs in cancer cells following incubation with lymphocytes or IFNγ, was abrogated under hypoxic conditions. HLA-preservation was linked wit...
Source: Cellular Immunology - March 20, 2024 Category: Allergy & Immunology Authors: Alexandra Giatromanolaki Georgios D Michos Erasmia Xanthopoulou Michael I Koukourakis Source Type: research

Identification of the major immune differences in severe asthmatic children according to their atopic dermatitis status
In conclusion, this study shows the major immune differences between cwSA with and without AD in BAL and blood suggesting that distinct endotypes may be implicated in the inflammatory responses observed in these pediatric patients.PMID:38428350 | DOI:10.1016/j.cellimm.2024.104815 (Source: Cellular Immunology)
Source: Cellular Immunology - March 1, 2024 Category: Allergy & Immunology Authors: Guillaume Lezmi Cl ément Poirault Marta Grauso C éline Dietrich Karine Adel-Patient Maria Leite-de-Moraes Source Type: research

Identification of the major immune differences in severe asthmatic children according to their atopic dermatitis status
In conclusion, this study shows the major immune differences between cwSA with and without AD in BAL and blood suggesting that distinct endotypes may be implicated in the inflammatory responses observed in these pediatric patients.PMID:38428350 | DOI:10.1016/j.cellimm.2024.104815 (Source: Cellular Immunology)
Source: Cellular Immunology - March 1, 2024 Category: Allergy & Immunology Authors: Guillaume Lezmi Cl ément Poirault Marta Grauso C éline Dietrich Karine Adel-Patient Maria Leite-de-Moraes Source Type: research

Identification of the major immune differences in severe asthmatic children according to their atopic dermatitis status
In conclusion, this study shows the major immune differences between cwSA with and without AD in BAL and blood suggesting that distinct endotypes may be implicated in the inflammatory responses observed in these pediatric patients.PMID:38428350 | DOI:10.1016/j.cellimm.2024.104815 (Source: Cellular Immunology)
Source: Cellular Immunology - March 1, 2024 Category: Allergy & Immunology Authors: Guillaume Lezmi Cl ément Poirault Marta Grauso C éline Dietrich Karine Adel-Patient Maria Leite-de-Moraes Source Type: research

Identification of the major immune differences in severe asthmatic children according to their atopic dermatitis status
In conclusion, this study shows the major immune differences between cwSA with and without AD in BAL and blood suggesting that distinct endotypes may be implicated in the inflammatory responses observed in these pediatric patients.PMID:38428350 | DOI:10.1016/j.cellimm.2024.104815 (Source: Cellular Immunology)
Source: Cellular Immunology - March 1, 2024 Category: Allergy & Immunology Authors: Guillaume Lezmi Cl ément Poirault Marta Grauso C éline Dietrich Karine Adel-Patient Maria Leite-de-Moraes Source Type: research

Identification of the major immune differences in severe asthmatic children according to their atopic dermatitis status
In conclusion, this study shows the major immune differences between cwSA with and without AD in BAL and blood suggesting that distinct endotypes may be implicated in the inflammatory responses observed in these pediatric patients.PMID:38428350 | DOI:10.1016/j.cellimm.2024.104815 (Source: Cellular Immunology)
Source: Cellular Immunology - March 1, 2024 Category: Allergy & Immunology Authors: Guillaume Lezmi Cl ément Poirault Marta Grauso C éline Dietrich Karine Adel-Patient Maria Leite-de-Moraes Source Type: research

Identification of the major immune differences in severe asthmatic children according to their atopic dermatitis status
In conclusion, this study shows the major immune differences between cwSA with and without AD in BAL and blood suggesting that distinct endotypes may be implicated in the inflammatory responses observed in these pediatric patients.PMID:38428350 | DOI:10.1016/j.cellimm.2024.104815 (Source: Cellular Immunology)
Source: Cellular Immunology - March 1, 2024 Category: Allergy & Immunology Authors: Guillaume Lezmi Cl ément Poirault Marta Grauso C éline Dietrich Karine Adel-Patient Maria Leite-de-Moraes Source Type: research

Identification of the major immune differences in severe asthmatic children according to their atopic dermatitis status
In conclusion, this study shows the major immune differences between cwSA with and without AD in BAL and blood suggesting that distinct endotypes may be implicated in the inflammatory responses observed in these pediatric patients.PMID:38428350 | DOI:10.1016/j.cellimm.2024.104815 (Source: Cellular Immunology)
Source: Cellular Immunology - March 1, 2024 Category: Allergy & Immunology Authors: Guillaume Lezmi Cl ément Poirault Marta Grauso C éline Dietrich Karine Adel-Patient Maria Leite-de-Moraes Source Type: research

Identification of the major immune differences in severe asthmatic children according to their atopic dermatitis status
In conclusion, this study shows the major immune differences between cwSA with and without AD in BAL and blood suggesting that distinct endotypes may be implicated in the inflammatory responses observed in these pediatric patients.PMID:38428350 | DOI:10.1016/j.cellimm.2024.104815 (Source: Cellular Immunology)
Source: Cellular Immunology - March 1, 2024 Category: Allergy & Immunology Authors: Guillaume Lezmi Cl ément Poirault Marta Grauso C éline Dietrich Karine Adel-Patient Maria Leite-de-Moraes Source Type: research