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159 records returned

The immunogenicity of the liposome-associated outer membrane proteins (OMPs) of Moraxella catarrhalis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The outer membrane proteins (OMPs) are the most immunogenic and attractive of the Moraxella catarrhalis vaccine antigens that may induce the protective immune response. The aim of this study was to determine the effectiveness of two types of OMP-associated phosphatidylcholine (PC) liposomal formulations (OMPs-PC, PC-OMPs) and of Zwittergent-based proteomicelles (OMPs-Z) in potentiating an anti-OMP systemic immune response in mice. The immunogenicities of the above preparations were evaluated by assessing serum anti-OMP IgG and IgA reactivity in the post-immunized mouse antisera using ELISA and Western blotting. Additio...
Source: Cellular and Molecular Biology Letters - October 22, 2009 Category: Biochemistry Authors: Augustyniak D, Mleczko J, Gutowicz J Tags: Cell Mol Biol Lett Source Type: journals

Establishing and functional characterization of an HEK-293 cell line expressing autofluorescently tagged beta-actin (pEYFP-ACTIN) and the neurokinin type 1 receptor (NK1-R).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study focused on establishing and making a comprehensive functional characterization of an HEK-293-transfected cell line that would coexpress the enhanced yellow fluorescent protein-actin (pEYFP-actin) construct and the neurokinin type 1 receptor (NK1-R), which is a member of the seven transmembrane (7TM) receptor family. In the initial selection procedure, the cloning ring technique was used alone, but failed to yield clones with homogenous pEYFP-actin expression. Flow cytometry sorting (FCS) was subsequently used to enrich the pEYFP-actin-expressing subpopulation of cells. The enzyme-linked immunosorbent assay (ELIS...
Source: Cellular and Molecular Biology Letters - October 14, 2009 Category: Biochemistry Authors: Hrovat A, Zavec AB, Pogačnik A, Frangež R, Vrecl M Tags: Cell Mol Biol Lett Source Type: journals

The intrabody targeting of hTERT attenuates the immortality of cancer cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and pr...
Source: Cellular and Molecular Biology Letters - September 22, 2009 Category: Biochemistry Authors: Zhu X, Yang N, Cai J, Yang G, Liang S, Ren D Tags: Cell Mol Biol Lett Source Type: journals

Important residue (G46) in erythroid spectrin tetramer formation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we replaced the Gly residue with Ala, Arg or Glu residues in an erythroid alpha-spectrin model protein to give G46A, G46R or G46E, respectively. We found that their association affinities with a beta-spectrin model protein were quite different from each other. G46R exhibited a 10-fold increase and G46E exhibited a 16-fold decrease, whereas G46A showed little difference, when compared with the wild type. The thermal and urea denaturation experiments showed insignificant structural change in G46R. Thus, the differences in affinity were due to differences in local, specific interactions, rather than conformatio...
Source: Cellular and Molecular Biology Letters - September 7, 2009 Category: Biochemistry Authors: Kang J, Song Y, Sevinc A, Fung LW Tags: Cell Mol Biol Lett Source Type: journals

Treatment with TNF-alpha and IFN-gamma alters the activation of SER/THR protein kinases and the metabolic response to IGF-I in mouse c2c12 myogenic cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion: i) TNF-alpha and IFN-gamma, but not IL-1beta, if present in the extracellular environment during C2C12 myoblast differentiation, prevent the stimulatory action of IGF-I on protein synthesis. ii) TNF-alpha- and IFN-gamma-induced IGF-I resistance of protein synthesis could be associated with the decreased phosphorylation of PKB and p70(S6k). iii) The activation of glucose uptake in C2C12 myogenic cells treated with IFN-gamma is PKB independent. iv) The similar effects of TNF-alpha and IFN-gamma on the signalling and action of IGF-I on protein synthesis in myogenic cells could suggest the involvement of both of...
Source: Cellular and Molecular Biology Letters - August 13, 2009 Category: Biochemistry Authors: Grzelkowska-Kowalczyk K, Wieteska-Skrzeczyńska W Tags: Cell Mol Biol Lett Source Type: journals

The age-dependent induction of apoptosis-inducing factor (AIF) in the human semitendinosus skeletal muscle.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To assess the dependence on age of the expression of apoptosis regulatory proteins in the human semitendinosus muscle, we measured the expression levels of several apoptosis-related genes, including apoptosis-inducing factor (AIF), Bax, Bcl-2, caspase-3 and heat shock protein 70 (HSP70), using RT-PCR, immunohistochemistry and TUNEL assays. We found that the DNA fragmentation was proportional to the age of the tissues sample donors. The expression levels of AIF were significantly elevated (by 10 to 25%) in semitendinosus tissue samples from older individuals, but the Bax, Bcl-2, caspase-3 and HSP 70 levels remained almo...
Source: Cellular and Molecular Biology Letters - August 13, 2009 Category: Biochemistry Authors: Park SY, Kim HY, Lee JH, Yoon KH, Chang MS, Park SK Tags: Cell Mol Biol Lett Source Type: journals

Increased expression of PAD2 after repeated intracerebroventricular infusions of soluble Abeta(25-35) in the Alzheimer's disease model rat brain: Effect of memantine.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we examined whether repeated intracerebroventricular infusions of soluble Abeta25-35 would affect the PAD2 expression in any regions of the rat brain. We also assessed the protective effect of memantine on Abeta-induced PAD2 alterations. The infusion of Abeta(25-35) increased the activity and protein level of PAD2 in the hippocampus, and co-treatment with memantine suppressed these changes. An immunohistochemical analysis showed that an increased level of PAD2 was coincident with GFAP-positive astrocytes and CD11b-positive microglia. In addition, immunofluoresecence staining revealed that citrullinepostive i...
Source: Cellular and Molecular Biology Letters - July 28, 2009 Category: Biochemistry Authors: Arif M, Kato T Tags: Cell Mol Biol Lett Source Type: journals

Molecular characterization of the niaD and pyrG genes from Penicillium camemberti, and their use as transformation markers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the nitrate reductase (niaD) and orotidine-5'-monophosphate decarboxylase (pyrG) genes from Penicillium camemberti were characterized, and their suitability as metabolic molecular markers for transformation was evaluated. The genes were amplified using PCR-related techniques, and sequenced. The niaD gene is flanked by the nitrite reductase (niiA) gene in a divergent arrangement, being part of the putative nitrate assimilation cluster in P. camemberti. pyrG presents several polymorphisms compared with a previously sequenced pyrG gene from another P. camemberti strain, but almost all are silent mutations. Sout...
Source: Cellular and Molecular Biology Letters - June 26, 2009 Category: Biochemistry Authors: Navarrete K, Roa A, Vaca I, Espinosa Y, Navarro C, Chávez R Tags: Cell Mol Biol Lett Source Type: journals

Overexpression of human osteopontin increases cell proliferation and migration in human embryo kidney-293 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Malignant tumors are characterized by dysregulated cell growth and the metastasis of secondary tumors. Numerous studies have documented that osteopontin (OPN) plays a key role in regulating tumor progression and metastasis. Here, we show that the overexpression of OPN in human embryo kidney-293 cells significantly increases both the level of cell proliferation, by provoking the G(1)/S transition, and the level of cell migration in vitro. These findings suggest that augmented OPN contributes to cell growth and motility. Inhibiting OPN or the pathway it stimulates may therefore represent a novel approach for the treatmen...
Source: Cellular and Molecular Biology Letters - June 26, 2009 Category: Biochemistry Authors: Liu YJ, Zhang DQ, Sui XM, Tian W Tags: Cell Mol Biol Lett Source Type: journals

The transcriptional regulation of Podocin (NPHS2) by Lmx1b and a promoter single nucleotide polymorphism.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Podocin (NPHS2) is a component of the glomerular slit membrane with major regulatory functions in the renal permeability of proteins. A loss of podocin and a decrease in its resynthesis can influence the outcome of renal diseases with nephrotic syndrome, such as minimal change glomerulonephritis, focal segmental glomerulosclerosis (FSGS) and membranous nephropathy. The transcriptional regulation of podocin may play a major role in these processes. We defined the transcriptional regulation of the human podocin gene and the influence of single nucleotide polymorphisms (SNPs) within its promoter region in the podocytes us...
Source: Cellular and Molecular Biology Letters - June 26, 2009 Category: Biochemistry Authors: Harendza S, Stahl RA, Schneider A Tags: Cell Mol Biol Lett Source Type: journals

Transcriptional profiles during the differentiation and maturation of monocyte-derived dendritic cells, analyzed using focused microarrays.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Dendritic cells (DC) are professional antigen-presenting cells capable of initiating primary immune responses. They have been intensively studied and are used in both basic immunology research and clinical immunotherapy. However, the genetic pathways leading to DC differentiation and maturation remain poorly understood. Using focused microarrays with oligonucletotide probes for 120 genes encoding co-stimulatory molecules, chemokines, chemokine receptors, cytokines, cytokine receptors, TLRs, and several other related molecules, we analyzed the kinetics of gene expression for the overall differentiation process of monocy...
Source: Cellular and Molecular Biology Letters - June 24, 2009 Category: Biochemistry Authors: Zhong W, Fei M, Zhu Y, Zhang X Tags: Cell Mol Biol Lett Source Type: journals

The influence of JNK and P38 MAPK inhibition on IL-12P40 and IL-23 production depending on IL12B promoter polymorphism.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The interleukin-12p40 gene (IL12B) encodes the p40 polypeptide chain, which, together with p19, composes IL-23. A bi-allelic promoter polymorphism (IL12Bpro) located at -2703 bp of the transcription initiation site has been reported to show associations with IL-12p40 production. To elucidate the dependence of IL-12p40 and IL-23 production on IL12Bpro polymorphism in relation to MAPK signal transduction pathways, we examined the effect of JNK and p38 inhibition on the secretion of these cytokines by stimulated peripheral blood mononuclear cells (PBMC) from healthy donors with 1.1 and 1.2/2.2 IL12Bpro genotypes. Stimulat...
Source: Cellular and Molecular Biology Letters - June 24, 2009 Category: Biochemistry Authors: Dobreva ZG, Stanilova SA, Miteva LD Tags: Cell Mol Biol Lett Source Type: journals

Mechanisms for the formation of membranous nanostructures in cell-to-cell communication.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cells interact by exchanging material and information. Two methods of cell-to-cell communication are by means of microvesicles and by means of nanotubes. Both microvesicles and nanotubes derive from the cell membrane and are able to transport the contents of the inner solution. In this review, we describe two physical mechanisms involved in the formation of microvesicles and nanotubes: curvature-mediated lateral redistribution of membrane components with the formation of membrane nanodomains; and plasmamediated attractive forces between membranes. These mechanisms are clinically relevant since they can be affected by d...
Source: Cellular and Molecular Biology Letters - June 24, 2009 Category: Biochemistry Authors: Schara K, Janša V, Suštar V, Dolinar D, Pavlič JI, Lokar M, Kralj-Iglič V, Veranič P, Iglič A Tags: Cell Mol Biol Lett Source Type: journals

The immunosuppressive activities of newly synthesized azaphenothiazines in human and mouse models.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we evaluated the activities of new types of azaphenothiazines in the following immunological assays: the proliferative response of human peripheral blood mononuclear cells induced by phytohemagglutin A or anti-CD3 antibodies; lipopolysaccharide-induced cytokine production by human PBMC; the secondary, humoral immune response in mice to sheep erythrocytes (in vitro); and delayed-type hypersensitivity in mice to ovalbumin (in vivo). In some tests, chlorpromazine served as a reference drug. The compounds exhibited differential inhibitory activities in the proliferation tests, with 10H-2,7-diazaphenothiazine (co...
Source: Cellular and Molecular Biology Letters - June 24, 2009 Category: Biochemistry Authors: Zimecki M, Artym J, Kocięba M, Pluta K, Morak-Młodawska B, Jeleń M Tags: Cell Mol Biol Lett Source Type: journals

Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we found that SFRS9 is a target for PRMT1-mediated arginine methylation in vitro, and that it is immunoprecipitated from HEK-293 lysates by antibodies that recognize both mono- and dimethylated arginines. We further observed that upon treatment with the methylation inhibitor Adox, the fluorescent EGFP-SFRS9 re-localizes to dot-like structures in the cell nucleus. In subsequent confocal analyses, we found that EGFP-SFRS9 localizes to nucleoli in Adox-treated cells. Our findings indicate the importance of arginine methylation for the subnuclear localization of SFRS9. PMID: 19557313 [PubMed - as supplied by...
Source: Cellular and Molecular Biology Letters - June 24, 2009 Category: Biochemistry Authors: Bressan GC, Moraes EC, Manfiolli AO, Kuniyoshi TM, Passos DO, Gomes MD, Kobarg J Tags: Cell Mol Biol Lett Source Type: journals

The anti-apoptotic activity of albumin for endothelium is inhibited by advanced glycation end products restricting intramolecular movement.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to determine if this inhibits the anti-apoptotic activity of HSA, and if such inactivation could be reversed by CNBr fragmentation. HSA-AGE was prepared by incubating HSA with glucose, and assessed using the fructosamine assay, mass spectrometry, SDS-PAGE and fluorometry. Low levels of AGE in the HSA had little effect upon its anti-apoptotic activity, but when the levels of AGE were high and the intra-molecular movement was reduced, endothelial cell survival was also found to be reduced to levels equivalent to those in cultures without HSA or serum (p > 0.001). Survival was restored by the inclusion of ...
Source: Cellular and Molecular Biology Letters - May 29, 2009 Category: Biochemistry Authors: Zoellner H, Siddiqui S, Kelly E, Medbury H Tags: Cell Mol Biol Lett Source Type: journals

The transcriptional regulation and cell-specific expression of the MAPK-activated protein kinase MK5.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the transcription pattern of MK5 in different tissues, and studied the kinetics of MK5 expression at the transcriptional and/or translation level in PC12 cells exposed to arsenite, forskolin, KCl, lipopolysaccharide, spermine NONOate, retinoic acid, serum, phorbol ester, temperature shock, and vanadate. Cells exposed to forskolin display a transient increase in MK5 mRNA, despite their unaltered MK5 protein levels. The MK5 promoters of human, mouse and rat contain a cAMP-responsive element that binds the cAMPresponsive element-binding protein (CREB) in vitro. Luciferase reporter constructs containing an 850-base...
Source: Cellular and Molecular Biology Letters - May 29, 2009 Category: Biochemistry Authors: Gerits N, Shiryaev A, Kostenko S, Klenow H, Shiryaeva O, Johannessen M, Moens U Tags: Cell Mol Biol Lett Source Type: journals

The functional antagonist Met-RANTES: A modified agonist that induces differential CCR5 trafficking.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CC chemokine receptor 5 (CCR5) is a pro-inflammatory chemokine receptor that is expressed on cells of the immune system, and specializes in cell migration in response to inflammation and tissue damage. Due to its key role in cell communication and migration, this receptor is involved in various inflammatory and autoimmune diseases, in addition to HIV infection. Met-RANTES is a modified CCR5 ligand that has previously been shown to antagonize CCR5 activation and function in response to its natural ligands in vitro. In vivo, Met-RANTES is able to reduce inflammation in models of induced inflammatory and autoimmune diseas...
Source: Cellular and Molecular Biology Letters - May 13, 2009 Category: Biochemistry Authors: Kiss DL, Longden J, Fechner GA, Avery VM Tags: Cell Mol Biol Lett Source Type: journals

The differentiation of human placenta-derived mesenchymal stem cells into dopaminergic cells in vitro.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mesenchymal stem cells (MSCs) constitute an interesting cellular source to promote brain regeneration after Parkinson's disease. MSCs have significant advantages over other stem cell types, and greater potential for immediate clinical application. The aim of this study was to investigate whether MSCs from the human placenta could be induced to differentiate into dopaminergic cells. MSCs from the human placenta were isolated by digestion and density gradient fractionation, and their cell surface glycoproteins were analyzed by flow cytometry. These MSCs were cultured under conditions promoting differetiation into adipocy...
Source: Cellular and Molecular Biology Letters - April 29, 2009 Category: Biochemistry Authors: Chen L, He DM, Zhang Y Tags: Cell Mol Biol Lett Source Type: journals

The expression of inducible nitric oxide synthase (iNOS) in the testis and epididymis of rats with a dihydrotestosterone (DHT) deficiency.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, our data clearly indicates that a finasterideinduced DHT deficiency intensifies the constitutive expression of iNOS in most rat testicular and epididymal cells, so it can be expected that the expression of inducible nitric oxide synthase (iNOS) could be regulated by DHT. On the other hand, the profile of the circulating DHT and T levels strongly suggests that the regulation of constitutive iNOS expression is complex and needs more detailed study. PMID: 19404589 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology Letters)
Source: Cellular and Molecular Biology Letters - April 28, 2009 Category: Biochemistry Authors: Kolasa A, Marchlewicz M, Kurzawa R, Głąbowski W, Trybek G, Wenda-Różewicka L, Wiszniewska B Tags: Cell Mol Biol Lett Source Type: journals

Capsaicin induces apoptosis by generating reactive oxygen species and disrupting mitochondrial transmembrane potential in human colon cancer cell lines.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, our results clearly showed that capsaicin induced apoptosis in colon cancer cells. Although the actual mechanisms of capsaicin-induced apoptosis remain uncertain, it may be a beneficial agent for colon cancer treatment and chemoprevention. PMID: 19381455 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology Letters)
Source: Cellular and Molecular Biology Letters - April 15, 2009 Category: Biochemistry Authors: Yang KM, Pyo JO, Kim GY, Yu R, Han IS, Ju SA, Kim WH, Kim BS Tags: Cell Mol Biol Lett Source Type: journals

The transcriptional cascade associated with creatine kinase down-regulation and mitochondrial biogenesis in mice sarcoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed at investigating the transcriptional cascade of CK down-regulation in carcinogen-induced sarcoma in mouse muscle. Both CK deficiency and enhanced nitric oxide synthase (NOS) were known to augment mitochondrial biogenesis, so we also explored the activation of the transcriptional cascade of mitochondrial biogenesis in this cancer. We observed the activation of the TNF-alpha-mediated nitric oxide production pathway with NFkappaB activation and concomitant degradation of MyoD and myogenin mRNA. Exploration of mitochondrial biogenesis revealed high cytochrome c oxidase activity and mitochondrial DNA content in...
Source: Cellular and Molecular Biology Letters - April 2, 2009 Category: Biochemistry Authors: Bera S, Ray M Tags: Cell Mol Biol Lett Source Type: journals

RNA interference against Biot2, a novel mouse testis - specific gene, inhibits the growth of tumor cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Biot2 is a novel murine testis-specific gene that was first identified using the SEREX technique, and named by our laboratory. Using conventional RT-PCR and real time RT-PCR, we tested the expression profile of Biot2 in normal tissues and various murine tumor cell lines. Using RNA interference, we studied the biological function of Biot2 in tumorigenesis. We applied various types of growth assay, such as the in vitro MTT, colony-forming and BrdU incorporation assays, along with in vivo tumorigenicity assays, to reveal its inhibition of tumor cell proliferation. The results revealed that the Biot2 transcript was detecte...
Source: Cellular and Molecular Biology Letters - March 10, 2009 Category: Biochemistry Authors: Wang CT, Zhang P, Wang YS, Ruan XZ, Li ZY, Peng F, Yang HS, Wei YQ Tags: Cell Mol Biol Lett Source Type: journals

Chronic increases in sphingosine kinase-1 activity induce a pro-inflammatory, pro-angiogenic phenotype in endothelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Sphingosine kinase-1 (SK1) promotes the formation of sphingosine-1-phosphate (S1P), which has potent pro-inflammatory and pro-angiogenic effects. We investigated the effects of raised SK1 levels on endothelial cell function and the possibility that this signaling pathway is activated in rheumatoid arthritis. Human umbilical vein endothelial cells with 3- to 5-fold SK1 (EC(SK)) overexpression were generated by adenoviral and retroviralmediated gene delivery. The activation state of these cells and their ability to undergo angiogenesis was determined. S1P was measured in synovial fluid from patients with RA and OA. EC(SK...
Source: Cellular and Molecular Biology Letters - February 23, 2009 Category: Biochemistry Authors: Limaye V, Xia P, Hahn C, Smith M, Vadas MA, Pitson SM, Gamble JR Tags: Cell Mol Biol Lett Source Type: journals

The effects of markedly raised intracellular sphingosine kinase-1 activity in endothelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The enzyme sphingosine kinase-1 (SK1) promotes the formation of sphingosine-1-phosphate (S1P), which is an important survival factor for endothelial cells (EC). Modest increases in intracellular SK1 activity in the EC are known to confer a survival advantage upon the cells. Here, we investigated the effects of more dramatic increases in intracellular SK1 in the EC. We found that these cells show reduced cell survival under conditions of stress, enhanced caspase-3 activity, cell cycle inhibition, and cell-cell junction disruption. We propose that alterations in the phosphorylation state of the enzyme may explain the dif...
Source: Cellular and Molecular Biology Letters - February 23, 2009 Category: Biochemistry Authors: Limaye V, Vadas MA, Pitson SM, Gamble JR Tags: Cell Mol Biol Lett Source Type: journals

The expression of protein kinase B in gastric cancer cell apoptosis induced by 12-O-tetradecanoylphorbol-1, 3-acetate.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Protein kinase B (PKB/Akt) is a serine-threonine kinase functioning downstream of phosphatidylinositol 3-kinase (PI-3 kinase) in response to mitogen or growth factor stimulation. In several cell types, it plays an important anti-apoptotic role. TPA is a potent regulator of the growth of many different cell types. Here, we detected that TPA could induce cell apoptosis in the gastric cancer cell line, BGC-823. We also found that TPA inhibited the expression of PKB/Akt in a TPA concentration- and time-dependent manner. Furthermore, TPA inhibited the phosphorylation of PKB at Ser473, but did not affect the phosphorylation ...
Source: Cellular and Molecular Biology Letters - February 23, 2009 Category: Biochemistry Authors: Zhang B, Xia C Tags: Cell Mol Biol Lett Source Type: journals

The effects of disodium pamidronate on human polymorphonuclear leukocytes and platelets: An in vitro study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, we hypothesized that treatment with pamidronate could stimulate NO-production by cells present near the bone compartment, thus constituting a protective mechanism against bone resorption occurring during inflammation. In addition, PMN- and platelet-derived NO could act as a negative feed-back signal to restrict the inflammatory processes. PMID: 19238333 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology Letters)
Source: Cellular and Molecular Biology Letters - February 23, 2009 Category: Biochemistry Authors: Salvolini E, Orciani M, Vignini A, Di Primio R, Mazzanti L Tags: Cell Mol Biol Lett Source Type: journals

The expression of COX-2, hTERT, MDM2, LATS2 and S100A2 in different types of non-small cell lung cancer (NSCLC).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Several studies have reported different expression levels of certain genes in NSCLC, mostly related to the stage and advancement of the tumours. We investigated 65 stage I-III NSCLC tumours: 32 adenocarcinomas (ADC), 26 squamous cell carcinomas (SCC) and 7 large cell carcinomas (LCC). Using the real-time reverse transcription polymerase chain reaction (RT-PCR), we analysed the expression of the COX-2, hTERT, MDM2, LATS2 and S100A2 genes and researched the relationships between the NSCLC types and the differences in expression levels. The differences in the expression levels of the LATS2, S100A2 and hTERT genes in diffe...
Source: Cellular and Molecular Biology Letters - February 23, 2009 Category: Biochemistry Authors: Stražišar M, Mlakar V, Glavač D Tags: Cell Mol Biol Lett Source Type: journals

Interactions between canthaxanthin and lipid membranes - possible mechanisms of canthaxanthin toxicity.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Canthaxanthin (beta, beta-carotene 4, 4' dione) is used widely as a drug or as a food and cosmetic colorant, but it may have some undesirable effects on human health, mainly caused by the formation of crystals in the macula lutea membranes of the retina. This condition is called canthaxanthin retinopathy. It has been shown that this type of dysfunction of the eye is strongly connected with damage to the blood vessels around the place of crystal deposition. This paper is a review of the experimental data supporting the hypothesis that the interactions of canthaxanthin with the lipid membranes and the aggregation of this...
Source: Cellular and Molecular Biology Letters - February 12, 2009 Category: Biochemistry Authors: Sujak A Tags: Cell Mol Biol Lett Source Type: journals

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A protocol for the induction of regeneration from leaves of Helichrysum italicum was established. Calli were found to form on the basal medium only when it was supplemented with thidiazuron (TDZ) alone or in combination with naphthalene acetic acid (NAA), with a percentage ranking of at least 80%. The hormone-free medium showed the highest percentage of shoot regeneration (62%) even though no callus formed. AFLP markers were employed to verify tissue culture-induced variation in the regenerated plantlets obtained by direct shoot regeneration or the indirect shoot regeneration process (callus formation). Seven out of th...
Source: Cellular and Molecular Biology Letters - February 4, 2009 Category: Biochemistry Authors: Perrini R, Alba V, Ruta C, Morone-Fortunato I, Blanco A, Montemurro C Tags: Cell Mol Biol Lett Source Type: journals

Potassium currents in human myogenic cells from healthy and congenital myotonic dystrophy foetuses.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The whole-cell patch clamp technique was used to record potassium currents in in vitro differentiating myoblasts isolated from healthy and myotonic dystrophy type 1 (DM1) foetuses carrying 2000 CTG repeats. The fusion of the DM1 myoblasts was reduced in comparison to that of the control cells. The dystrophic muscle cells expressed less voltage-activated K(+) (delayed rectifier and non-inactivating delayed rectifier) and inward rectifier channels than the age-matched control cells. However, the resting membrane potential was not significantly different between the control and the DM1 cells. After four days in a differen...
Source: Cellular and Molecular Biology Letters - February 4, 2009 Category: Biochemistry Authors: Nurowska E, Constanti A, Dworakowska B, Mouly V, Furling D, Lorenzon P, Pietrangelo T, Dołowy K, Ruzzier F Tags: Cell Mol Biol Lett Source Type: journals

Targeted cationic poly(D,L-lactic-co-glycolic acid) nanoparticles for gene delivery to cultured cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We developed a new targeted cationic nanoparticulate system composed of poly(D,L-lactic-co-glycolic acid) (PLGA), 1,2-dioleoyl-3-(trimethylammonium) propane (DOTAP) and asialofetuin (AF), and found it to be a highly effective formulation for gene delivery to liver tumor cells. The nanoparticles (NP) were prepared by a modified solvent evaporation process that used two protocols in order to encapsulate (NP1 particles) or adsorb (NP2 particles) plasmid DNA. The final particles are in the nanoscale range. pDNA loaded in PLGA/DOTAP/AF particles with high loading efficiency showed a positive surface charge. Targeted asialof...
Source: Cellular and Molecular Biology Letters - February 4, 2009 Category: Biochemistry Authors: Díez S, Miguéliz I, Tros de Ilarduya C Tags: Cell Mol Biol Lett Source Type: journals

Acheron, an novel la antigen family member, binds to cask and forms a complex with id transcription factors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19183863 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology Letters)
Source: Cellular and Molecular Biology Letters - January 28, 2009 Category: Biochemistry Authors: Weng H, Kim C, Valavanis C, Wang Z, Schwartz LM Tags: Cell Mol Biol Lett Source Type: journals

Activation of the heat shock response in a primary cellular model of motoneuron neurodegeneration-evidence for neuroprotective and neurotoxic effects.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pharmacological up-regulation of heat shock proteins (hsps) rescues motoneurons from cell death in a mouse model of amyotrophic lateral sclerosis. However, the relationship between increased hsp expression and neuronal survival is not straightforward. Here we examined the effects of two pharmacological agents that induce the heat shock response via activation of HSF-1, on stressed primary motoneurons in culture. Although both arimoclomol and celastrol induced the expression of Hsp70, their effects on primary motoneurons in culture were significantly different. Whereas arimoclomol had survival-promoting effects, rescuin...
Source: Cellular and Molecular Biology Letters - January 28, 2009 Category: Biochemistry Authors: Kalmar B, Greensmith L Tags: Cell Mol Biol Lett Source Type: journals

The knockdown of c-myc expression by RNAi inhibits cell proliferation in human colon cancer HT-29 cells in vitro and in vivo.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We investigated the effects of RNA interference-mediated silencing of the c-myc gene on celluar proliferation and apoptosis in human colon cancer HT-29 cells in vitro and in vivo. A small interfering RNA (siRNA) targeting c-myc was designed, the DNA template was synthesized, and the siRNA was obtained by in vitro transcription. After siRNA transfection into HT-29 and human neuroblastoma IMR-32 cells with Lipofectamine 2000trade mark, the proliferation of the HT-29 and IMR-32 cells was assessed via 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) colorimetry, and Hoechst 33258 staining was used to ...
Source: Cellular and Molecular Biology Letters - January 28, 2009 Category: Biochemistry Authors: Zhang X, Ge YL, Tian RH Tags: Cell Mol Biol Lett Source Type: journals

A transcriptionally active copia-like retroelement in Citrus limon.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The plant nuclear genome is largely composed of mobile DNA, which can rearrange genomes and other individual gene structure and also affect gene regulation through various promoted activities: transposition, insertion, excision, chromosome breakage, and ectopic recombination. Ty1-copia-like retrotransposon is a widespread class of transposable elements in the plant kingdom, representing a large part of the total DNA content. Here, a novel retrotransposon-like sequence was isolated and identified as the Ty1-copia-like reverse transcriptase domain (named here CLCoy1), based on the homology of known elements. Fluorescence...
Source: Cellular and Molecular Biology Letters - December 24, 2008 Category: Biochemistry Authors: De Felice B, Wilson RR, Argenziano C, Kafantaris I, Conicella C Tags: Cell Mol Biol Lett Source Type: journals

Toll-like receptors and their role in carcinogenesis and anti-tumor treatment.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Toll-like receptors (TLRs) have been described as major components of the innate immune system, recognizing the conserved molecular structures found in the large groups of pathogens called pathogen-associated molecular patterns (PAMPs). TLR expression is ubiquitous, from epithelial to immunocompetent cells. TLR ligation triggers several adapter proteins and downstream kinases, leading to the induction of key pro-inflammatory mediators but also anti-inflammatory and anti-tumor cytokines. The result of this activation goes beyond innate immunity to shape the adaptive responses against pathogens and tumor cells, and maint...
Source: Cellular and Molecular Biology Letters - December 18, 2008 Category: Biochemistry Authors: Wolska A, Lech-Marańda E, Robak T Tags: Cell Mol Biol Lett Source Type: journals

Acheron, an novel la antigen family member, binds to cask and forms a complex with id transcription factors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Acheron, a Lupus antigen ortholog, was identified as a novel death-associated transcript from the intersegmental muscles of the mothManduca sexta. Acheron is phylogenetically-conserved and represents a new sub-family of Lupus antigen proteins. Acheron is expressed predominantly in neurons and muscle in vertebrates, and regulates several developmental events including myogenesis, neurogenesis and possibly metastasis. Using Acheron as bait, we performed a yeast two-hybrid screen with a mouse embryo cDNA library and identified CASK-C, a novel CASK/Lin-2 isoform, as an Acheron binding partner. Acheron and CASK-C bind via t...
Source: Cellular and Molecular Biology Letters - December 18, 2008 Category: Biochemistry Authors: Weng H, Kim C, Valavanis C, Wang Z, Schwartz LM Tags: Cell Mol Biol Lett Source Type: journals

The signaling pathways of Epstein-Barr virus-encoded latent membrane protein 2A (LMP2A) in latency and cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Epstein-Barr virus (EBV) is a ubiquitous virus with infections commonly resulting in a latency carrier state. Although the exact role of EBV in cancer pathogenesis remains not entirely clear, it is highly probable that it causes several lymphoid and epithelial malignancies, such as Hodgkin's lymphoma, NK-T cell lymphoma, Burkitt's lymphoma, and nasopharyngeal carcinoma. EBV-associated malignancies are associated with a latent form of infection, and several of these EBV-encoded latent proteins are known to mediate cellular transformation. These include six nuclear antigens and three latent membrane proteins. Studies hav...
Source: Cellular and Molecular Biology Letters - December 13, 2008 Category: Biochemistry Authors: Pang MF, Lin KW, Peh SC Tags: Cell Mol Biol Lett Source Type: journals

Perturbation of the lipid phase of a membrane is not involved in the modulation of MRP1 transport activity by flavonoids.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The expression of transmembrane transporter multidrug resistance-associated protein 1 (MRP1) confers the multidrug-resistant phenotype (MDR) on cancer cells. Since the activity of the other MDR transporter, P-glycoprotein, is sensitive to membrane perturbation, we aimed to check whether the changes in lipid bilayer properties induced by flavones (apigenin, acacetin) and flavonols (morin, myricetin) were related to their MRP1 inhibitory activity. All the flavonoids inhibited the efflux of MRP1 fluorescent substrate from human erythrocytes and breast cancer cells. Morin was also found to stimulate the ATPase activity of ...
Source: Cellular and Molecular Biology Letters - November 19, 2008 Category: Biochemistry Authors: Wesołowska O, Hendrich AB, Laniapietrzak B, Wiśniewski J, Molnar J, Ocsovszki I, Michalak K Tags: Cell Mol Biol Lett Source Type: journals

Oral cyclosporine A - the current picture of its liposomal and other delivery systems.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The discovery of cyclosporine A was a milestone in organ transplantation and the treatment of autoimmune diseases. However, developing an efficient oral delivery system for this drug is complicated by its poor biopharmaceutical characteristics (low solubility and permeability) and the need to carefully monitor its levels in the blood. Current research is exploring various approaches, including those based on emulsions, microspheres, nanoparticles, and liposomes. Although progress has been made, none of the formulations is flawless. This review is a brief description of the main pharmaceutical systems and devices that h...
Source: Cellular and Molecular Biology Letters - November 12, 2008 Category: Biochemistry Authors: Czogalla A Tags: Cell Mol Biol Lett Source Type: journals

The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [(3)H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C(14), C(18) or C(18...
Source: Cellular and Molecular Biology Letters - October 31, 2008 Category: Biochemistry Authors: Panasiewicz M, Domek H, Hoser G, Fedoryszak N, Kawalec M, Pacuszka T Tags: Cell Mol Biol Lett Source Type: journals

A consensus map of chromosome 6R in rye (Secale cereale L.).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Four F(2) mapping populations derived from crosses between rye inbred lines DS2xRXL10, 541xOt1-3, S120xS76 and 544xOt0-20 were used to develop a consensus map of chromosome 6R. Thirteen marker loci that were polymorphic in more than one mapping population constituted the basis for the alignment of the four maps using the JoinMap v. 3.0 software package. The consensus map consists of 104 molecular marker loci including RFLPs, RAPDs, AFLPs, SSRs, ISSRs, SCARs, STSs and isozymes. The average distance between the marker loci is 1.3 cM, and the total map length is 135.5 cM. This consensus map may be used as a source of mole...
Source: Cellular and Molecular Biology Letters - October 31, 2008 Category: Biochemistry Authors: Stojałowski S, Myśków B, Milczarski P, Masojć P Tags: Cell Mol Biol Lett Source Type: journals

Regulator of G-protein signalling expression and function in ovarian cancer cell lines.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Regulator of G-protein signalling (RGS)(2) proteins critically regulate signalling cascades initiated by G-protein coupled receptors (GPCRs) by accelerating the deactivation of heterotrimeric G-proteins. Lysophosphatidic acid (LPA) is the predominant growth factor that drives the progression of ovarian cancer by activating specific GPCRs and G-proteins expressed in ovarian cancer cells. We have recently reported that RGS proteins endogenously expressed in SKOV-3 ovarian cancer cells dramatically attenuate LPA stimulated cell signalling. The goal of this study was twofold: first, to identify candidate RGS proteins expre...
Source: Cellular and Molecular Biology Letters - October 31, 2008 Category: Biochemistry Authors: Hurst JH, Mendpara N, Hooks SB Tags: Cell Mol Biol Lett Source Type: journals

THE GTPase domain of Galphao contributes to the functional interaction of Galphao with the promyelocytic leukemia zinc finger protein.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we determined that the GTPase domain of Galphao contributes to Galphao:PLZF interaction. We also showed that the Galphao GTPase domain is important in modulating the function of PLZF. This data indicates that the GTPase domain of Galphao may be necessary for the functional interaction of Galphao with PLZF. PMID: 18953495 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology Letters)
Source: Cellular and Molecular Biology Letters - October 16, 2008 Category: Biochemistry Authors: Won JH, Ghil SH Tags: Cell Mol Biol Lett Source Type: journals

Galectin-1 expression in innervated and denervated skeletal muscle.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we compared the expression of galectin-1 mRNA and immunoreactivity in innervated and denervated mouse and rat hind-limb and hemidiaphragm muscles. The results show that galectin-1 mRNA expression and immunoreactivity are up-regulated following denervation. The galectin-1 mRNA is expressed in the extrasynaptic and perisynaptic regions of the muscle, and its immunoreactivity can be detected in both regions by Western blot analysis. The results are compatible with a role for galectin-1 in facilitating reinnervation of denervated skeletal muscle. PMID: 18850073 [PubMed - as supplied by publisher] (Source: Ce...
Source: Cellular and Molecular Biology Letters - October 10, 2008 Category: Biochemistry Authors: Svensson A, Tågerud S Tags: Cell Mol Biol Lett Source Type: journals

Doxorubicin-transferrin conjugate selectively overcomes multidrug resistance in leukaemia cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, DOX-TRF was able to overcome MDR in the leukaemia cell lines while having only a very limited effect on normal tissue cells. PMID: 18850074 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology Letters)
Source: Cellular and Molecular Biology Letters - October 10, 2008 Category: Biochemistry Authors: Lubgan D, Jóźwiak Z, Grabenbauer GG, Distel LV Tags: Cell Mol Biol Lett Source Type: journals

Homing of annexin-labeled stem cells to apoptotic cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report here on an original method that employs PS-ANXA5 conjugation to target stem cells to apoptotic cells. Mesenchymal stem cells (MSCs) from GFP-positive transgenic rats were biotinylated on membrane surfaces with sulfosuccinimidyl-6-(biotinamido) hexanoate (sulfo-NHS-LC-biot) and then bound to avidin. The avidin-biotinylated MSCs were labeled with biotin conjugated ANXA5. Bovine aortic endothelial cells (BAE-1 cells) were exposed to UVC to induce caspasedependent apoptosis. Finally, we tested the ability of ANXA5-labeled MSCs to bind BAE-1 apoptotic cells: suspended ANXA5-labeled MSCs were seeded for 1 hour on a mon...
Source: Cellular and Molecular Biology Letters - October 6, 2008 Category: Biochemistry Authors: Gerasimou A, Ramella R, Brero A, Boero O, Sheiban I, Levi R, Gallo MP Tags: Cell Mol Biol Lett Source Type: journals

The carnitine acetyltransferase gene (CRAT): A characterization of porcine transcripts with insights into the 5'-end variants of mammalian transcripts and their possible sub-cellular localization.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Carnitine acetyltransferase (CRAT) is an important enzyme for energy homeostasis and fat metabolism. We characterized the predicted full length cDNA sequence of the porcine CRAT gene. Its structure is very similar to that in humans with respect to the size and organization of the 14 exons. We demonstrated the existence of a porcine alternative transcript resulting from a partial intron-retention at the 5' end of exon 2. To perform a comparison of the 5' end variants of the mammalian CRAT gene, we analyzed the Genbank data, and here we propose a new 5' variant for dog, rat and mouse. In contrast to other mammals where t...
Source: Cellular and Molecular Biology Letters - October 6, 2008 Category: Biochemistry Authors: Robic A, Faraut T, Liaubet L, Milan D Tags: Cell Mol Biol Lett Source Type: journals

The interaction between L1-type proteins and ankyrins - a master switch for L1-type CAM function.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
L1-type cell adhesion molecules (CAMs) are important mediators of neural differentiation, including axonal outgrowth and pathfinding and also of synapse formation and maintenance. In addition, their interactions with cytoskeletal components are highly conserved and regulated. How these different aspects of CAM functionality relate to each other is not well understood. Based on results from our and other laboratories we propose that ankyrin-binding to L1-type CAMs provides a master switch. The interaction with ankyrins directs L1-type adhesive proteins into different functional contexts, either ankyrin-independent funct...
Source: Cellular and Molecular Biology Letters - October 6, 2008 Category: Biochemistry Authors: Hortsch M, Nagaraj K, Godenschwege TA Tags: Cell Mol Biol Lett Source Type: journals