Cellular and Molecular ImmunologyThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

Get the very latest Swine Flu news via the MedWorm Swine Flu RSS news feed - updated hourly from thousands of authoritative health and news sources.

• Contents
• Discussions

Pages: 1  2  3  4  

Modulation of neuroimmune responses on glia in the central nervous system: implication in therapeutic intervention against neuroinflammation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It has long been known that the brain is an immunologically privileged site in normal conditions. Although the cascade of immune responses can occur as long as there is a neuronal injury or a potent immune stimulation, how the brain keeps glial cells in a quiescent state is still unclear. Increasing efforts have been made by several laboratories to elucidate how repression of immune responses is achieved in the neuronal environment. The suppression factors include neurotransmitters, neurohormones, neurotrophic factors, anti-inflammatory factors, and cell-cell contact via adhesion molecules or CD200 receptor. This revie...
Source: Cellular and Molecular Immunology - October 1, 2009 Category: Molecular Biology Authors: Chang RC, Chiu K, Ho YS, So KF Tags: Cell Mol Immunol Source Type: journals

NF-kappaB signaling pathway, inflammation and colorectal cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
There is growing evidence for a connection between inflammation and tumor development, and the nuclear factor kappa B (NF-kappaB), a proinflammatory transcription factor, is hypothesized to promote tumorigenesis. Although the genetic evidence for the hypothesis has been lacking, recent papers have lent credence to this hypothesis. It has been reported that constitutive NF-kappaB activation in inflammatory bowel diseases (IBDs) increases risk of colorectal cancer (CRC) in the patients with the number of years of active disease. NF-kappaB activation might induce cellular transformation, mediate cellular proliferation, pr...
Source: Cellular and Molecular Immunology - October 1, 2009 Category: Molecular Biology Authors: Wang S, Liu Z, Wang L, Zhang X Tags: Cell Mol Immunol Source Type: journals

Modulatory effects and action mechanisms of tryptanthrin on murine myeloid leukemia cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, tryptanthrin was shown to suppress the proliferation of the murine myeloid leukemia WEHI-3B JCS cells in a dose- and time-dependent manner. It also significantly reduced the growth of WEHI-3B JCS cells in vivo in syngeneic BALB/c mice. However, it exhibited no significant direct cytotoxicity on normal murine peritoneal macrophages. Flow cytometric analysis showed an obvious cell cycle arrest of the tryptanthrin-treated WEHI-3B JCS cells at the G0/G1 phase. The expression of cyclin D2, D3, Cdk 2, 4 and 6 genes in WEHI-3B JCS cells was found to be down-regulated at 24 h as measured by RT-PCR. Morphological and...
Source: Cellular and Molecular Immunology - October 1, 2009 Category: Molecular Biology Authors: Chan HL, Yip HY, Mak NK, Leung KN Tags: Cell Mol Immunol Source Type: journals

MicroRNA-155 regulates inflammatory cytokine production in tumor-associated macrophages via targeting C/EBPbeta.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Macrophages (Mphi) are prominent components of solid tumors and exhibit distinct phenotypes in different microenvironments. We have recently found that tumors can alter the normal developmental process of Mphi to trigger transient activation of monocytes, but the underlying regulatory mechanisms are incompletely understood. Here, we showed that the protein expression of transcription factor C/EBPbeta was markedly elevated in tumor-associated Mphi both in vitro and human tumors in situ. The expression of C/EBP protein correlated with cytokine production in tumor-activated monocytes. Moreover, we found that C/EBPbeta exp...
Source: Cellular and Molecular Immunology - October 1, 2009 Category: Molecular Biology Authors: He M, Xu Z, Ding T, Kuang DM, Zheng L Tags: Cell Mol Immunol Source Type: journals

Leptin signaling protects NK cells from apoptosis during development in mouse bone marrow.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we have characterized NK cell differentiation and maturation in the BM of leptin-receptor deficient db/db mice at a prediabetic stage. Although the BM cellularity was similar to the control value, the total number of NK cells was severely reduced in mutant mice. Flow cytometric analysis of db/db BM cells revealed significantly decreased frequencies of developing NK cells at various stages of differentiation. BM db/db NK cells displayed markedly increased apoptosis but maintained normal cell cycling status and proliferative capacity. Moreover, recombinant leptin could significantly enhance the survival of NK ...
Source: Cellular and Molecular Immunology - October 1, 2009 Category: Molecular Biology Authors: Lo CK, Lam QL, Yang M, Ko KH, Sun L, Ma R, Wang S, Xu H, Tam S, Wu CY, Zheng BJ, Lu L Tags: Cell Mol Immunol Source Type: journals

Elevated levels of serum IgA against Saccharomyces cerevisiae mannan in patients with rheumatoid arthritis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was undertaken to investigate whether levels of anti-Saccharomyces cerevisiae mannan antibodies (ASCMA) in human sera, a marker for several autoimmune diseases, correlate with rheumatoid arthritis (RA). ASCMA-IgA, -IgG and -IgM levels were measured with enzyme linked immunosorbent assays (ELISA) in patients with RA (n = 30) and 152 healthy adult controls. ASCMA-IgA prevalence was significantly higher in RA patients (40%) than in healthy subjects (5.3%). A strong correlation between levels of ASCMA-IgA and CRP (r = 0.695; p < 0.01) and ESR (r = 0.708; p < 0.01) in RA patients was observed. No significant di...
Source: Cellular and Molecular Immunology - October 1, 2009 Category: Molecular Biology Authors: Dai H, Li Z, Zhang Y, Lv P, Gao XM Tags: Cell Mol Immunol Source Type: journals

EBV-induced human CD8(+) NKT cells synergise CD4(+) NKT cells suppressing EBV-associated tumours upon induction of Th1-bias.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we have established a human-thymus-SCID chimaera model to further investigate the underlying mechanism of EBV-induced CD8(+) NKT cells in suppressing EBV-associated malignancies. In the human-thymus-SCID chimera, EBV-induced CD8(+) NKT cells suppress EBV-associated malignancies in a manner dependent on the Th1-bias response and syngeneic CD3(+) T cells. However, adoptive transfer with CD4(+) NKT cells alone inhibits T cell immunity. Interestingly, CD4(+) NKT cells themselves secrete high levels of IL-2, enhancing the persistence of adoptively transferred CD8(+) NKT cells and T cells, thereby leading to a mor...
Source: Cellular and Molecular Immunology - October 1, 2009 Category: Molecular Biology Authors: Xiao W, Li L, Zhou R, Xiao R, Wang Y, Ji X, Wu M, Wang L, Huang W, Zheng X, Tan X, Chen L, Xiong T, Xiong J, Jin Y, Tan J, He Y Tags: Cell Mol Immunol Source Type: journals

Inhibition of NF-kappaB expression and allergen-induced airway inflammation in a mouse allergic asthma model by andrographolide.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Andrographolide from traditional Chinese herbal medicines previously showed it possesses a strong anti-inflammatory activity. In present study, we investigated whether Andrographolide could inhibit allergen-induced airway inflammation and airways hyper-responsiveness and explored the mechanism of Andrographolide on allergen-induced airway inflammation and airways hyper-responsiveness. After sensitized and challenged by ovalbumin, the BALB/c mice were administered intraperitoneally with Andrographolide. Hyper-responsiveness was recorded. The lung tissues were assessed by histological examinations. NF-kappaB in lung was ...
Source: Cellular and Molecular Immunology - October 1, 2009 Category: Molecular Biology Authors: Li J, Luo L, Wang X, Liao B, Li G Tags: Cell Mol Immunol Source Type: journals

A critical role of activin A in maturation of mouse peritoneal macrophages in vitro and in vivo.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Activin A, a multifunctional factor of the transforming growth factor-beta (TGF-beta) superfamily, is mainly produced by microglia and macrophages, and its anti-inflammatory and pro-inflammatory activities are both related to macrophage functions. However the direct effect of activin A on the rest macrophages in vivo remains unclear. In the present study, the results showed that activin A not only increased NO and IL-1beta release, but also promoted phagocytic abilities of mouse peritoneal macrophages in vitro and in vivo, whereas it did not influence MHC I and MHC II expression. Moreover, we found that activin A signi...
Source: Cellular and Molecular Immunology - October 1, 2009 Category: Molecular Biology Authors: Wang Y, Cui X, Tai G, Ge J, Li N, Chen F, Yu F, Liu Z Tags: Cell Mol Immunol Source Type: journals

Specifically binding of L-ficolin to N-glycans of HCV envelope glycoproteins E1 and E2 leads to complement activation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
L-ficolin, one of lectin families, is a recently identified complement factor that initiates lectin pathway of complement. Little is known about its role in viral hepatitis. In the present study, we found that L-ficolin in serum from 103 patients with hepatitis C virus (HCV), were significantly higher than that in 150 healthy controls. We further found that L-ficolin expressions were significantly increased in vitro study by HCV JFH-1 infected human hepatocyte cell line Huh7.5.1. Investigation of the mechanisms of the L-ficolin action on HCV demonstrated that L-ficolin protein could recognize and bind to envelope glyco...
Source: Cellular and Molecular Immunology - July 31, 2009 Category: Molecular Biology Authors: Liu J, Ali MA, Shi Y, Zhao Y, Luo F, Yu J, Xiang T, Tang J, Li D, Hu Q, Ho W, Zhang X Tags: Cell Mol Immunol Source Type: journals

Purification and molecular identification of an antifungal peptide from the hemolymph of Musca domestica (housefly).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study the anterior tip of the larvae of housefly was cut off with a pair of fine scissors and hemolymph was collected and exuded in an ice-cold test tube. From the hemolymph an antifungal substance was isolated by solid-phase extraction combined with reverse phase-high performance liquid chromotography (RP-HPLC) and named as Musca domestica antifungal peptide-1 (MAF-1). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed its molecular weight was 17 kDa. UV absorption spectra revealed that this antifungal substance possessed the characteristics of protein peptides. Analysis by fingerprint-id...
Source: Cellular and Molecular Immunology - July 31, 2009 Category: Molecular Biology Authors: Fu P, Wu J, Guo G Tags: Cell Mol Immunol Source Type: journals

Inefficiency of C3H/HeN mice to control chlamydial lung infection correlates with downregulation of neutrophil activation during the late stage of infection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We previously reported that massive infiltration of neutrophils in C3H/HeN (C3H) mice could not efficiently control Chlamydia muridarum (Cm) infection and might contribute to the high susceptibility of these mice to lung infection. To further define the nature of neutrophil responses in C3H mice during chlamydial infection, we examine the expression of adhesion molecules and CD11b related to neutrophils infiltration and activation, respectively, following intranasal Cm infection. The results showed that the expression of selectins (E-selectin, P-selectin and L-selectin), and intercellular cell adhesion molecule-1 (ICAM...
Source: Cellular and Molecular Immunology - July 31, 2009 Category: Molecular Biology Authors: Tang X, Bu X, Zhang N, Li X, Huang H, Bai H, Yang X Tags: Cell Mol Immunol Source Type: journals

Recombinant expression of a novel human transcriptional repressor HMBOX1 and preparation of anti-HMBOX1 monoclonal antibody.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
HMBOX1 was a novel transcription factor possibly involving in function of pancreas and cytotoxicity of NK cells. For function determination, recombinant human HMBOX1 protein was obtained and purified, and the monoclonal antibodies against HMBOX1 were prepared. The full-length cDNA fragment encoding HMBOX1 was amplified from NK-92 cells and inserted into prokaryotic expression vector pET22b. The pET22b-HMBOX1-6his vector was then transformed into E. coli Rosetta (DE3) and induced by 1 mM IPTG for 4 h at 37 degrees Celsius. The fusion HMBOX1 protein was mainly expressed in inclusion bodies, which was purified and refolde...
Source: Cellular and Molecular Immunology - July 31, 2009 Category: Molecular Biology Authors: Dai J, Wu L, Zhang C, Zheng X, Tian Z, Zhang J Tags: Cell Mol Immunol Source Type: journals

EBV LMP2A-specific T cell immune responses elicited by dendritic cells loaded with LMP2A protein.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study provides a rationale for the DC-based immunotherapy against EBV-LMP2A expressing malignancies. PMID: 19728928 [PubMed - in process] (Source: Cellular and Molecular Immunology)
Source: Cellular and Molecular Immunology - July 31, 2009 Category: Molecular Biology Authors: Chen Y, Sun H, Liu G, Wang B, Wang F, Sun B, Yao K Tags: Cell Mol Immunol Source Type: journals

Effects of transdermal estrogen therapy on expressions of estrogen receptors and T-lymphocyte apoptosis in surgically menopausal women.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Studies have demonstrated estrogen replacement therapy can improve the life quality of surgically menopausal women. However, the mechanisms in this process remain poorly defined. Here we show the effect of transdermal estrogen therapy on expressions of estrogen receptors and T-lymphocyte apoptosis in surgically postmenopausal women. Fifteen surgically menopausal women, 15 naturally menopausal women and 15 young women were chosen in our studies. Peripheral vein blood was collected and serum E2 and FSH levels were assessed using ACCESS. T-lymphocyte apoptosis and the expressions of Fas, FasL and ER subtypes and were dete...
Source: Cellular and Molecular Immunology - July 31, 2009 Category: Molecular Biology Authors: Zhang J, Chen X, Zhang S, Zhou G, Xia X, Lu L Tags: Cell Mol Immunol Source Type: journals

RGD-FasL induces apoptosis in hepatocellular carcinoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, RGD-FasL appears to be a low-toxicity selective inducer of tumor cell death, which merits further investigation in preclinical and clinical studies. Furthermore, this approach offers a versatile technology for complexing target ligands with therapeutic recombinant proteins. To distinguish the anti-tumor effects of FasL in vivo, tumor and liver tissues were harvested to examine for evidence of necrotic cells, tumor cells, or apoptotic cells by Hematoxylin and eosin (HE) staining. PMID: 19728930 [PubMed - in process] (Source: Cellular and Molecular Immunology)
Source: Cellular and Molecular Immunology - July 31, 2009 Category: Molecular Biology Authors: Liu Z, Wang J, Yin P, Qiu J, Liu R, Li W, Fan X, Cheng X, Chen C, Zhang J, Zhuang G Tags: Cell Mol Immunol Source Type: journals

Expression, purification and characterization of C-FADD.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, mouse FADD (80-205) containing DD domain and C-terminal region, designated as C-FADD, was expressed in E. coli with His-tag at the N-terminus and purified by Ni2+ affinity chromatography. The purified protein existed as a homogenous monomer in glutaraldehyde cross-linking analysis and exhibited a typical alpha-helix spectrum in CD (circular dichroism) assay. In vitro His-tag pull-down assay demonstrated that the purified C-FADD possessed the CK Ialpha-binding activity which was important for its non-apoptotic function. PMID: 19728931 [PubMed - in process] (Source: Cellular and Molecular Immunology)
Source: Cellular and Molecular Immunology - July 31, 2009 Category: Molecular Biology Authors: Chen Y, Ma D, Huang QL, Zheng W, Zhang J, Shen Y, Li J, Dong W, Lu M, Wang J, Hua ZC Tags: Cell Mol Immunol Source Type: journals

HLA B27 as predisposition factor to suffer age related macular degeneration.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To research whether specific alleles HLA class I (HLA-A and HLA-B) and class II (HLA-DR) are risk factors for the development of exudative type of Age Related Macular Degeneration (ARMD), HLA antigens are expressed both in normal and affected eyes with ARMD. We designed a prospective case-controlled study. We recruited 75 patients with choroidal neovascularization predominantly classic or occult, secondary to ARMD, and treated with photodynamic therapy. Two hundred and fifty patients over 55 years old, without ophthalmologic pathology who went to hospital for an analytical routine check were used as control. The analys...
Source: Cellular and Molecular Immunology - July 31, 2009 Category: Molecular Biology Authors: Becerril EV, Fernández RG, Torres LP, Lacomba MS, Galera JM Tags: Cell Mol Immunol Source Type: journals

Expression of redox factor-1 in early injury period after liver transplantation in rat model.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aims of this study were to observe the relationship between injury of graft and expression of redox factor-1 (Ref-1) in early period (24 h) after liver transplantation in rat model. One hundred and fifty adult male Wister rats were randomly divided into three groups including liver transplant group, sham surgery group and untreated control group. After liver transplantation, animals were sacrificed at different time points, and the changes and significance of the expression of Ref-1 were then explored by immunohistochemistry, serology and histopathology. As compared with sham surgery group and untreated control gro...
Source: Cellular and Molecular Immunology - July 31, 2009 Category: Molecular Biology Authors: Zhang P, Du X, Sun Z, Xu L Tags: Cell Mol Immunol Source Type: journals

Phenotype and function of monocyte-derived dendritic cells from chinese rhesus macaques.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, we have characterized the phenotype and ultimate function of MDDCs from CRM for the first time. PMID: 19567198 [PubMed - in process] (Source: Cellular and Molecular Immunology)
Source: Cellular and Molecular Immunology - May 31, 2009 Category: Molecular Biology Authors: Xia H, Liu H, Zhang G, Zheng Y Tags: Cell Mol Immunol Source Type: journals

Combination of human Fas (CD95/Apo-1) ligand with adriamycin significantly enhances the efficacy of antitumor response.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The prognosis of hepatocellular carcinoma (HCC) is poor, even with the combined treatment of curative resection and adjuvant chemoradiotherapy. To solve this problem, many biologic therapies have been investigated. Fas ligand (FasL, CD95L) is mainly expressed in activated T lymphocytes and natural killer (NK) cells, and plays a central role in both cell-mediated immunity and immune downregulation. Several studies have shown that FasL is expressed in HCC. In the present report, we prepared recombinant human pET-22b(+)/FasL protein and investigated the effect of FasL on HCC cells in vitro and on tumor growth in a murine ...
Source: Cellular and Molecular Immunology - May 31, 2009 Category: Molecular Biology Authors: Liu Z, Liu R, Qiu J, Yin P, Luo F, Su J, Li W, Chen C, Fan X, Zhang J, Zhuang G Tags: Cell Mol Immunol Source Type: journals

PI3K/AKT mediated p53 down-regulation participates in CpG DNA inhibition of spontaneous B cell apoptosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we showed B cell apoptosis was significantly inhibited by addition of CpG DNA. Treatment of CpG DNA could reduce the expression of caspase 3, increase IAP and Bcl-xL expressions, and inhibit p53 protein expression which level was increased in B cell spontaneous apoptosis at 24 h. AKT kinase activity was increased with the incubation of CpG DNA. The wortmannin and Ly294002 could abrogate the protection of B cell from apoptosis by CpG DNA. The up-regulations of Bcl-xL and IAP by CpG DNA were not inhibited when blocking PI3K by specific inhibitor Ly294002, while the inhibition of p53 by CpG DNA could be blocked...
Source: Cellular and Molecular Immunology - May 31, 2009 Category: Molecular Biology Authors: Zhou Y, Zhen H, Mei Y, Wang Y, Feng J, Xu S, Fu X Tags: Cell Mol Immunol Source Type: journals

The mechanism of chemokine receptor 9 internalization triggered by interleukin 2 and interleukin 4.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we preliminarily investigated the mechanism of internalization of CCR9 on MOLT4 cell model (a human leukemia T-cell line, naturally expresses CCR9) and found that IL-2 upregulated the cell surface expression of IL-4Ralpha (CD124) greatly, whereas IL-4 had no significant influence on alpha (CD25) and beta subunits (CD122) of IL-2R. Moreover, specific inhibitors, such as staurosporine, H89 and heparin, inhibited internalization of CCR9, which indicated a role of protein kinase C (PKC) and G protein-coupled kinase 2 (GRK2), respectively. Furthermore, GRK2 was upregulated and translocated to cell membrane in IL-...
Source: Cellular and Molecular Immunology - May 31, 2009 Category: Molecular Biology Authors: Tong X, Zhang L, Zhang L, Hu M, Leng J, Yu B, Zhou B, Hu Y, Zhang Q Tags: Cell Mol Immunol Source Type: journals

H. pylori escape host immunoreaction through inhibiting ILK expression by VacA.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Helicobacter pylori (H. pylori) persistently colonizes the gastric mucosa despite a vigorous immune response. Vacuolating cytotoxin secreted by H. pylori has turned out to be a potent immunomodulatory toxin, but the signal transduction pathways involved has not been studied in macrophages. We observed in this study that vacA-deficient H. pylori induced significantly higher expression of integrin-linked kinase (ILK) and endothelial nitric oxygen synthase (eNOS), and significantly more production of reactive oxygen species (ROS) in monocyte/macrophage-like U937 cells, as compared with isogenic vacA+ H. pylori. The expres...
Source: Cellular and Molecular Immunology - May 31, 2009 Category: Molecular Biology Authors: Yuan J, Li P, Tao J, Shi X, Hu B, Chen H, Guo X Tags: Cell Mol Immunol Source Type: journals

Down-regulation of TLR9 expression affects the maturation and function of murine bone marrow-derived dendritic cells induced by CpG.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Toll-like receptor 9 (TLR9) is expressed intracellularly by dendritic cells (DCs) and specifically recognizes unmethylated CpG motif. Recognition of TLR9 to CpG DNA can induce DC maturation followed by the subsequent immune responses. Here, RNA interference (RNAi) was used to identify the effect of CpG DNA signaling on DC function. The results showed that transfection of DCs with siRNA specific for TLR9 gene significantly down-regulated TLR9 expression. Immature DCs transfected with TLR9 siRNA did not differentiate into mature DCs with exposure to CpG. TLR9 siRNA-treated DCs expressed low levels of MHC II and CD40 with...
Source: Cellular and Molecular Immunology - May 31, 2009 Category: Molecular Biology Authors: Ma L, Zhao G, Hua C, Li X, Zhao X, Sun L, Hou Y Tags: Cell Mol Immunol Source Type: journals

Expression of IL-10 and TNF-alpha in rats with cerebral infarction after transplantation with mesenchymal stem cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study strongly suggested transplantation with MSCs could reduce neuronal injury post focal cerebral ischemia in rats partly by regulating the expressions of IL-10 and TNF-alpha in the brain. PMID: 19567204 [PubMed - in process] (Source: Cellular and Molecular Immunology)
Source: Cellular and Molecular Immunology - May 31, 2009 Category: Molecular Biology Authors: Liu N, Chen R, Du H, Wang J, Zhang Y, Wen J Tags: Cell Mol Immunol Source Type: journals

Expression of IL-1alpha, IL-6, TGF-beta, FasL and ZNF265 during sertoli cell infection by ureaplasma urealyticum.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To investigate immunoregulatory mechanisms of Sertoli cells in the testis in vitro and in vivo, we utilized our well-characterized Ureaplasma Urealyticum (UU)-induced model. We investigated the expressions of IL-1alpha, IL-6, TGF-beta, FasL and ZNF265 at the first, second and third weeks post-infection. During recovery from inflammation and with the help of negative regulators TGF-beta and FasL, the high levels of IL-1alpha and IL-6 expressions were observed in the early stages of the infection, and decreased gradually in the later weeks both in vitro and in vivo. The trend of varied expression of ZNF265 was similar to...
Source: Cellular and Molecular Immunology - May 31, 2009 Category: Molecular Biology Authors: Li R, Xi Y, Liu X, Chen G, Wang B, Jiang L, Li W Tags: Cell Mol Immunol Source Type: journals

Differential responses between monocytes and monocyte-derived macrophages for lipopolysaccharide stimulation of calves.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this experiment Toll-like receptor expression pattern in monocytes and monocyte-derived macrophages by lipopolysaccharide (LPS) stimulation was examined. Jugular venous blood was collected from four Japanese calves, and the peripheral blood mononuclear cells (PBMCs) were isolated. The cells were directly used for collecting monocytes by magnetic cell sorting or cultured for 7 days to collect monocyte-derived macrophages in Repcell. Then we analyzed the mRNA expression pattern of TLRs and cytokines in monocytes and monocyte-derived macrophages after LPS stimulation for 24 h. LPS stimulation of both monocytes and mono...
Source: Cellular and Molecular Immunology - May 31, 2009 Category: Molecular Biology Authors: Guo Y, Zhao G, Tanaka S, Yamaguchi T Tags: Cell Mol Immunol Source Type: journals

Chronic obstructive pulmonary disease: evidence for an autoimmune component.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chronic obstructive pulmonary disease (COPD) is characterized by an irreversible limitation on pulmonary airflow associated with chronic inflammation and mucous hypersecretion (chronic bronchitis) and/or the pathological destruction of alveolar airspaces leading to emphysema. COPD, predominantly as a result of tobacco smoke exposure, represents the fourth leading cause of mortality worldwide and its prevalence is increasing. Despite this, much of the basic mechanisms which contribute to disease progression remain to be elucidated and current therapeutic approaches are, for the most part, based upon alleviating patient ...
Source: Cellular and Molecular Immunology - March 31, 2009 Category: Molecular Biology Authors: Stefanska AM, Walsh PT Tags: Cell Mol Immunol Source Type: journals

Dendritic cells transduced with SOCS1 gene exhibit regulatory DC properties and prolong allograft survival.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we constructed adenoviral vector coding SOCS1 (Ad-SOCS1) that can efficiently increase SOCS1 gene expression in bone marrow-derived dendritic cells. DCs transduced with Ad-SOCS1 (DC-SOCS1) expressed low levels of costimulatory and MHC molecules, were resistant to maturation and activation stimulation, induced allogeneic T-cell hyporesponsiveness, and promoted the generation of regulatory-like T cells in vitro. DC-SOCS1 pretreatment significantly prolonged the survival of allografts and led to a substantial increase in the generation of regulatory T cells. Our data suggest that SOCS1 inhibits DC maturation an...
Source: Cellular and Molecular Immunology - March 31, 2009 Category: Molecular Biology Authors: Fu H, Song S, Liu F, Ni Z, Tang Y, Shen X, Xiao L, Ding G, Wang Q Tags: Cell Mol Immunol Source Type: journals

Computational prediction and identification of Epstein-Barr virus latent membrane protein 2A antigen-specific CD8+ T-cell epitopes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we predicted six HLA-A2 restricted CTL candidate epitopes of LMP2A by SYFPEITHI, NetMHC and MHCPred methods combined with the polynomial method. Subsequently, biological functions of these peptides were tested by experiments in vitro. In ELISPOT assay, the positive response of the LMP2A specific CTL stimulated by three (LMP2A(264-272), LMP2A(426-434) and LMP2A(356-364)) of six peptides respectively showed that the numbers of spots forming cells (SFC) ranged from 55.7 to 80.6 SFC/50,000 CD8(+) T cells and the responding index (RI) ranged from 5.4 to 7. These three epitope-specific CTLs could effectively kill ...
Source: Cellular and Molecular Immunology - March 31, 2009 Category: Molecular Biology Authors: Wang B, Yao K, Liu G, Xie F, Zhou F, Chen Y Tags: Cell Mol Immunol Source Type: journals

Analysis of the conservation of T cell receptor alpha and beta chain variable regions gene in pp65 peptide-specific HLA-A*0201-restricted CD8+ T cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we further analyzed the restrictive usage of TCR V alpha and V beta gene families and investigated the CDR3 gene sequence of pp65 peptide-specific CD8(+) T cells. Analysis of CDR3 spectratypes suggested a restricted usage of TCR alpha chain AV8, AV12, AV21, AV31 families and TCR beta chain BV3, BV14, BV21, BV23, BV11 families in donor CD8(+) T cells stimulated by pp65 peptide. The sequences of these T cells involved similar sequence (TX) G (X) A in CDR3 region of TCR alpha chain and L (XT) G (X) A in TCR beta chain. PMID: 19403059 [PubMed - in process] (Source: Cellular and Molecular Immunology)
Source: Cellular and Molecular Immunology - March 31, 2009 Category: Molecular Biology Authors: Luo W, Ma L, Wen Q, Zhou M, Wang X Tags: Cell Mol Immunol Source Type: journals

Acute morphine administration reduces cell-mediated immunity and induces reactivation of latent herpes simplex virus type 1 in BALB/c mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the effect of acute morphine administration on the reactivation of latent herpes was investigated in a mouse model. Because of the important role of cytolytic T lymphocyte (CTL) activity in the inhibition of herpes simplex virus type 1 (HSV-1) reactivation, the effect of acute morphine administration on CTL responses was also evaluated. Furthermore, lymphocyte proliferation and IFN-gamma production were evaluated for their roles in the induction of the CTL response. The findings showed that acute morphine administration significantly reduced CTL responses, lymphocyte proliferation, and IFN-gamma production. ...
Source: Cellular and Molecular Immunology - March 31, 2009 Category: Molecular Biology Authors: Mojadadi S, Jamali A, Khansarinejad B, Soleimanjahi H, Bamdad T Tags: Cell Mol Immunol Source Type: journals

Construction of soluble Mamu-b*1703, a class I major histocompatibility complex of Chinese rhesus macaques, monomer and tetramer loaded with a simian immunodeficiency virus peptide.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We reported in this study the procedure for preparation of a soluble Mamu-B*1703 (a MHC class I molecule of Chinese macaques) monomer and tetramer loaded with a dominant simian immunodeficiency virus (SIV) epitope IW9 (IRYPKTFGW) that was identified to be Mamu-B*1701-restricted in Indian macaques. The DNA fragment encoding the Mamu-B*1703 extracellular domain fused with a BirA substrate peptide (BSP) was amplified from a previously cloned cDNA and inserted into a prokaratic expression vector. In the presence of the antigenic peptide IW9 and light chain beta2-microglobulin, the expressed heavy chain was refolded into a solu...
Source: Cellular and Molecular Immunology - March 31, 2009 Category: Molecular Biology Authors: Ouyang D, Wang X, He X, Xu L, Shi H, Gao Q, Guo H Tags: Cell Mol Immunol Source Type: journals

CTLA4 silencing with siRNA promotes deviation of Th1/Th2 in chronic hepatitis B patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To determine whether RNA interference (RNAi) could block cytotoxic T-lymphocyte antigen 4 (CTLA4) in human lymphocytes in vitro and promote IFN-gamma and IL-2 secretions, three small interfering RNAs (siRNAs) were selected based on target specificity sequences of human CTLA4 and transfected into human lymphocytes of chronic HBV patients. As a result, the expression of human CTLA4 mRNA was efficiently suppressed by all the three siRNAs. Compared with negative control (siRNA-co), siRNA-1 inhibited the expression of CTLA4 most efficiently and was used in the further study. The expressions of IFN-gamma and IL-2 were upregu...
Source: Cellular and Molecular Immunology - March 31, 2009 Category: Molecular Biology Authors: Yu Y, Wu H, Tang Z, Zang G Tags: Cell Mol Immunol Source Type: journals

Direct effects of activin A on the activation of mouse macrophage RAW264.7 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we reported a direct effect of activin A as a pro-inflammatory factor on mouse macrophage cell line RAW264.7 cells. Our data revealed that activin A could not only increase IL-1 beta and IL-6 production from RAW264.7 cells, but also promote pinocytic and phagocytic activities of RAW264.7 cells. In addition, activin A obviously up-regulated MHC II expression on the surface of RAW264.7 cells, whereas did not influence MHC I expression. Activin A also enhanced CD80 expression, which is a marker of activated macrophages, but did not influence RAW264.7 cell proliferation. These data suggest that activin A may reg...
Source: Cellular and Molecular Immunology - March 31, 2009 Category: Molecular Biology Authors: Ge J, Wang Y, Feng Y, Liu H, Cui X, Chen F, Tai G, Liu Z Tags: Cell Mol Immunol Source Type: journals

Computational prediction of the specificities of proteasome interaction with antigen protein.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the processing and presentation of antigenic peptides bound by the major histocompatibility complex (MHC) class I molecule, the ubiquitin-proteasome system of the eukaryotic cells plays an important role in proteolysis and degradation. The ubiquitinated protein substrate is delivered into the 26S proteasome to be digested and degraded. The proteasome degrading substrate is actually protein-protein interactions. Some researches of predicting proteasome cleave site rarely gave the information of the proteasome interacting with its substrate, and so the accuracy and reliability of these proteasome cleavage predictive m...
Source: Cellular and Molecular Immunology - March 31, 2009 Category: Molecular Biology Authors: Liu T, Liu W, Song Z, Jiao C, Zhu M, Wang X Tags: Cell Mol Immunol Source Type: journals

Plasma levels of monocyte chemotactic protein 3 and beta-nerve growth factor increase with amnestic mild cognitive impairment.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we used multiplex cytokine assay to assess the plasma levels of 22 cytokines in patients with MCI subtyped as amnestic and non-amnestic, according to cognitive features. When comparing the levels of plasma growth factors, chemokines and cytokines, plasma levels of monocyte chemotactic protein 3 (MCP-3), and beta-nerve growth factor (beta-NGF) in these two groups, they were found to be significantly higher in amnestic MCI patients than in non-amnestic MCI patients, after adjusting for age and gender. This suggests that plasma MCP-3 and beta-NGF may be useful in differentiating subtypes of MCI. PMID: 19403...
Source: Cellular and Molecular Immunology - March 31, 2009 Category: Molecular Biology Authors: Lee KS, Chung JH, Lee KH, Shin MJ, Oh BH, Lee SH, Hong CH Tags: Cell Mol Immunol Source Type: journals

Studies on the specific degranulation of mast cell sensitized by several allergens in vitro.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, an approach was taken to study the characteristics of sensitized mast cell degranulation in vitro, which associated with the study of mast cells and animal models. BALB/c mice were immunized respectively by several food allergens, then blood and peritoneal mast cells were collected at different time points. A dynamic determination was carried out between mast cells and serumal IgE. Comparative analysis on sequential time points showed that there was a close coincidence between mast cell degranulation and IgE antibody titers in sensitized BALB/c mice. Furthermore, it is interesting that sensitized mast cells ...
Source: Cellular and Molecular Immunology - March 31, 2009 Category: Molecular Biology Authors: Guo Y, Li Z, Lin H, Samee H, Khalid J Tags: Cell Mol Immunol Source Type: journals

Dynamic behavior and function of Foxp3+ regulatory T cells in tumor bearing host.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Regulatory T cells (Tregs) expressing forkhead/winged-helix transcription factor Foxp3 represent a distinct lineage of lymphocytes which play a central role in protecting the host from autoimmune diseases. However, Tregs also pose a major problem to anti-tumor immunity. Growing body of evidence from both laboratory and clinical investigations has demonstrated that expansion and accumulation of these immunosuppressive cells correlates with advanced tumor growth and predicts poor disease prognosis. How tumor development subverts normal self-tolerance function of Tregs thereby thwarts host anti-tumor immunity remains elus...
Source: Cellular and Molecular Immunology - February 1, 2009 Category: Molecular Biology Authors: Qin FX Tags: Cell Mol Immunol Source Type: journals

Blockade of Tim-3 pathway ameliorates interferon-gamma production from hepatic CD8+ T cells in a mouse model of hepatitis B virus infection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
T cell immunoglobulin- and mucin-domain-containing molecule-3 (Tim-3) has been reported to participate in the pathogenesis of inflammatory diseases. However, whether Tim-3 is involved in hepatitis B virus (HBV) infection remains unknown. Here, we studied the expression and function of Tim-3 in a hydrodynamics-based mouse model of HBV infection. A significant increase of Tim-3 expression on hepatic T lymphocytes, especially on CD8+ T cells, was demonstrated in HBV model mice from day 7 to day 18. After Tim-3 knockdown by specific shRNAs, significantly increased IFN-gamma production from hepatic CD8+ T cells in HBV model...
Source: Cellular and Molecular Immunology - February 1, 2009 Category: Molecular Biology Authors: Ju Y, Hou N, Zhang XN, Zhao D, Liu Y, Wang JJ, Luan F, Shi W, Zhu FL, Sun WS, Zhang LN, Gao CJ, Gao LF, Liang XH, Ma CH Tags: Cell Mol Immunol Source Type: journals

Dynamic behavior and function of foxp3(+) regulatory T cells in tumor bearing host.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Regulatory T cells (Tregs) expressing forkhead/winged-helix transcription factor Foxp3 represent a distinct lineage of lymphocytes which play a central role in protecting the host from autoimmune diseases. However, Tregs also pose a major problem to anti-tumor immunity. Growing body of evidence from both laboratory and clinical investigations has demonstrated that expansion and accumulation of these immunosuppressive cells correlates with advanced tumor growth and predicts poor disease prognosis. How tumor development subverts normal self-tolerance function of Tregs thereby thwarts host anti-tumor immunity remains elus...
Source: Cellular and Molecular Immunology - February 1, 2009 Category: Molecular Biology Authors: Qin FX Tags: Cell Mol Immunol Source Type: journals

Cell death mechanisms induced by cytotoxic lymphocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
One of the functions of the immune system is to recognize and destroy abnormal or infected cells to maintain homeostasis. This is accomplished by cytotoxic lymphocytes. Cytotoxicity is a highly organized multifactor process. Here, we reviewed the apoptosis pathways induced by the two main cytotoxic lymphocyte subsets, natural killer (NK) cells and CD8(+) T cells. In base to recent experimental evidence, we reviewed NK receptors involved in recognition of target-cell, as well as lytic molecules such as perforin, granzymes-A and -B, and granulysin. In addition, we reviewed the Fas-FasL intercellular linkage mediated path...
Source: Cellular and Molecular Immunology - February 1, 2009 Category: Molecular Biology Authors: Chavez-Galan L, Arenas-Del Angel MC, Zenteno E, Chavez R, Lascurain R Tags: Cell Mol Immunol Source Type: journals

Cloning and Characterization of DULP, a Novel Ubiquitin-Like Molecule from Human Dendritic Cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We identified a novel ubiquitin-like molecule DULP from human dendritic cells. DULP contains a domain that shares 26% identity and 34% similarity with ubiquitin, and it possesses the corresponding Ile-44 hydrophobic patch used by mono- or poly-ubiquitin to interact with a ubiquitin-interaction motif (UIM) or ubiquitin-associated domain (UBA). Lysine residue corresponding to 6 of ubiquitin, which is involved in the formation of a multi-ubiquitin chain that can bind proteasomal subunit Rpn10/S5a, is also conserved in its ubiquitin-homology domain. However, DULP does not possess the highly conserved C-terminus Gly-Gly req...
Source: Cellular and Molecular Immunology - February 1, 2009 Category: Molecular Biology Authors: Liu GY, Liu SX, Li P, Tang L, Han YM, An HZ, Li JY, Dai XK, Li N, Cao XT, Yu YZ Tags: Cell Mol Immunol Source Type: journals

Blockade of Tim-3 Pathway Ameliorates Interferon-gamma Production from Hepatic CD8(+) T Cells in a Mouse Model of Hepatitis B Virus Infection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
T cell immunoglobulin- and mucin-domain-containing molecule-3 (Tim-3) has been reported to participate in the pathogenesis of inflammatory diseases. However, whether Tim-3 is involved in hepatitis B virus (HBV) infection remains unknown. Here, we studied the expression and function of Tim-3 in a hydrodynamics-based mouse model of HBV infection. A significant increase of Tim-3 expression on hepatic T lymphocytes, especially on CD8(+) T cells, was demonstrated in HBV model mice from day 7 to day 18. After Tim-3 knockdown by specific shRNAs, significantly increased IFN-gamma production from hepatic CD8(+) T cells in HBV m...
Source: Cellular and Molecular Immunology - February 1, 2009 Category: Molecular Biology Authors: Ju Y, Hou N, Zhang XN, Zhao D, Liu Y, Wang JJ, Luan F, Shi W, Zhu FL, Sun WS, Zhang LN, Gao CJ, Gao LF, Liang XH, Ma CH Tags: Cell Mol Immunol Source Type: journals

Induction of Th1-Type Immune Response by Chitosan Nanoparticles Containing Plasmid DNA Encoding House Dust Mite Allergen Der p 2 for Oral Vaccination in Mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was to prepare the chitosan-pDer p 2 nanoparticles and to investigate the effect of chitosan-DNA nanoparticles on immune response in mice by oral delivery of chitosan-DNA nanoparticles. The nanoparticles were synthesized by complexing chitosan with plasmid DNA. The DNA was fully complexed into chitosan-DNA nanoparticles, suggesting a 100% encapsulation efficiency. Chitosan-DNA complex renders a significant protection of the plasmid. No effect on cell viability was observed in both cell types and average cell viability over 100% was obtained. Oral gene delivery with chitosan-DNA nanoparticles can generate a highe...
Source: Cellular and Molecular Immunology - February 1, 2009 Category: Molecular Biology Authors: Li GP, Liu ZG, Liao B, Zhong NS Tags: Cell Mol Immunol Source Type: journals

The Effects of Aerosolized STAT1 Antisense Oligodeoxynucleotides on Rat Pulmonary Fibrosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study investigated the effect of aerosolized STAT1 ASON on the expressions of inflammatory mediators, hydroxyproline and type I and type III collagen mRNA in BLM-induced rat pulmonary fibrosis. The results showed that STAT1 ASON applied by aerosolization could ameliorate alveolitis and fibrosis, inhibit the expressions of inflammatory mediators, decrease the content of hydroxyproline, and suppress the expressions of type I and type III collagen mRNA in lung tissue in BLM-induced rat pulmonary fibrosis. These results suggest that aerosolized STAT1 ASON might be considered as a promising new strategy in the treatment of...
Source: Cellular and Molecular Immunology - February 1, 2009 Category: Molecular Biology Authors: Wang WJ, Liao B, Zeng M, Zhu C, Fan XM Tags: Cell Mol Immunol Source Type: journals

CD59 Silencing via Retrovirus-Mediated RNA Interference Enhanced Complement-Mediated Cell Damage in Ovary Cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CD59, belonging to membrane complement regulatory proteins (mCRPs), inhibits the cytolytic activity of complement and is over-expressed in solid cancers, including ovary cancer. The aim of the present study was to construct recombinant retrovirus encoding shRNA targeted human CD59 and infect A2780 cells in order to investigate the relationship between decreased CD59 expression and tumorigenesis of ovary cancer. siCD59 and siCD59-C were successfully constructed and identified by PCR, restriction endonuclease analyses and DNA sequencing, respectively. The siCD59 was able to efficiently infect A2780 cells, which was confi...
Source: Cellular and Molecular Immunology - February 1, 2009 Category: Molecular Biology Authors: Shi XX, Zhang B, Zang JL, Wang GY, Gao MH Tags: Cell Mol Immunol Source Type: journals

Immunotherapeutical potential of mycobacterium vaccae on m. Tuberculosis infection in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Tuberculosis remains the worldwide infectious disease. To identify the therapeutic potential of M. vaccae in treating tuberculosis, M. vaccae was injected into Mycobacterium tuberculosis (M. tuberculosis) infected mice. The optimal dose of M. vaccae (22.5 mug/mouse) treated mice showed lower pathological change index, spleen weight index, lung weight index and vital M. tuberculosis count than those of the untreated group. Treatment with M. vaccae enhanced the percentages of CD3(+) and CD4(+) T cells, IFN-gamma(+)CD4(+) T cells, innate immune cells including NK cells, NK1.1(+) T cells and gammadeltaT cells, and reduced ...
Source: Cellular and Molecular Immunology - February 1, 2009 Category: Molecular Biology Authors: Xu LJ, Wang YY, Zheng XD, Gui XD, Tao LF, Wei HM Tags: Cell Mol Immunol Source Type: journals

Similar Ability of FbaA with M Protein to Elicit Protective Immunity Against Group A Streptococcus Challenge in Mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Group A streptococcus (GAS), an important human pathogen, can cause various kinds of infections including superficial infections and potentially lethal infections, and the search for an effective vaccine to prevent GAS infections has been ongoing for many years. This paper compares the immunogenicity and immunoprotection of FbaA (an Fn-binding protein expressed on the surface of GAS) with that of M protein, the best immunogen of GAS. Assay for immune response showed that FbaA, similar to M protein, could induce protein-specific high IgG titer in BALB/c mice. Furthermore, following GAS challenge, the mice immunized with...
Source: Cellular and Molecular Immunology - February 1, 2009 Category: Molecular Biology Authors: Ma CQ, Li CH, Wang XR, Zeng RH, Yin XL, Feng HD, Wei L Tags: Cell Mol Immunol Source Type: journals