Lysosomal Peptide Self-Assembly To Control Cell Behavior
Chembiochem. 2024 Apr 25:e202400232. doi: 10.1002/cbic.202400232. Online ahead of print.ABSTRACTLysosomes are membrane-enclosed organelles that play key roles in degrading and recycling cellular debris, cellular signaling, and energy metabolism processes. Confinement of amphiphilic peptides in the lysosome to construct functional nanostructures through noncovalent interactions is an emerging approach to tune the homeostasis of lysosome. After briefly introducing the importance of lysosome and its functions, we discuss the advantages of lysosomal nanostructure formation for disease therapy. We next discuss the strategy for ...
Source: Chembiochem - April 25, 2024 Category: Biochemistry Authors: Sangshuang Li Huaimin Wang Source Type: research
Endogenous Antimicrobial-Immunomodulatory Molecules: Networking Biomolecules of Innate Immunity
Chembiochem. 2024 Apr 24:e202400089. doi: 10.1002/cbic.202400089. Online ahead of print.ABSTRACTEndogenous antimicrobial-immunomodulatory molecules (EAIMs) are essential to immune-mediated human health and evolution. Conventionally, antimicrobial peptides (AMPs) have been regarded as the dominant endogenous antimicrobial molecule; however, AMPs are not sufficient to account for the full spectrum of antimicrobial-immunomodulatory duality occurring within the human body. The threat posed by pathogenic microbes is pervasive with the capacity for widespread impact across many organ systems and multiple biochemical pathways; ac...
Source: Chembiochem - April 24, 2024 Category: Biochemistry Authors: Donald F Weaver Source Type: research
Enterolyin S, a Polythiazole-containing Hemolytic Peptide from Enterococcus caccae
Chembiochem. 2024 Apr 22:e202400212. doi: 10.1002/cbic.202400212. Online ahead of print.ABSTRACTThe β-hemolytic factor streptolysin S (SLS) is an important linear azol(in)e-containing peptide (LAP) that contributes significantly to the virulence of Streptococcus pyogenes. Despite its discovery 85 years ago, SLS has evaded structural characterizing owing to its notoriously problematic physicochemical properties. Here, we report the discovery and characterization of a structurally analogous hemolytic peptide from Enterococcus caccae, termed enterolysin S (ELS). Through heterologous expression, site-directed mutagenesis, che...
Source: Chembiochem - April 22, 2024 Category: Biochemistry Authors: Chengyou Shi Varshal A Patel Douglas A Mitchell Huimin Zhao Source Type: research
Enterolyin S, a Polythiazole-containing Hemolytic Peptide from Enterococcus caccae
Chembiochem. 2024 Apr 22:e202400212. doi: 10.1002/cbic.202400212. Online ahead of print.ABSTRACTThe β-hemolytic factor streptolysin S (SLS) is an important linear azol(in)e-containing peptide (LAP) that contributes significantly to the virulence of Streptococcus pyogenes. Despite its discovery 85 years ago, SLS has evaded structural characterizing owing to its notoriously problematic physicochemical properties. Here, we report the discovery and characterization of a structurally analogous hemolytic peptide from Enterococcus caccae, termed enterolysin S (ELS). Through heterologous expression, site-directed mutagenesis, che...
Source: Chembiochem - April 22, 2024 Category: Biochemistry Authors: Chengyou Shi Varshal A Patel Douglas A Mitchell Huimin Zhao Source Type: research
Interactions of phenylalanine derivatives with human tyrosinase: lessons from experimental and theoretical studies
Chembiochem. 2024 Apr 20:e202400235. doi: 10.1002/cbic.202400235. Online ahead of print.ABSTRACTThe pigmentation of the skin, modulated by different actors in melanogenesis, is mainly due to the melanins (protective pigments). In humans, these pigments' precursors are synthetized by an enzyme known as tyrosinase (TyH). The regulation of the enzyme activity by specific modulators (inhibitors or activators) can offer a means to fight hypo- and hyper-pigmentations responsible for medical, psychological and societal handicaps. Herein, we report the investigation of phenylalanine derivatives as TyH modulators. Interacting with ...
Source: Chembiochem - April 20, 2024 Category: Biochemistry Authors: Clarisse Faure Yi Ming Ng Catherine Belle Montserrat Soler-Lopez Lyna Khettabi Melissa Saidi Nathalie Berthet Marc Maresca Christian Philouze Walid Rachidi Marius Reglier Amaury du Moullinet d'Hardemare H élène Jamet Source Type: research
A Platinum-Aluminum Bimetallic Salen Complex for Pro-senescence Cancer Therapy
Chembiochem. 2024 Apr 19:e202400105. doi: 10.1002/cbic.202400105. Online ahead of print.ABSTRACTCell senescence is defined as irreversible cell cycle arrest, which can be triggered by telomere shortening or by various types of genotoxic stress. Induction of senescence is emerging as a new strategy for the treatment of cancer, especially when sequentially combined with a second senolytic drug capable of killing the resulting senescent cells, however severely suffering from the undesired off-target side effects from the senolytic drugs. Here, we prepare a bimetalic platinum-aluminum salen complex (Alumiplatin) for cancer the...
Source: Chembiochem - April 19, 2024 Category: Biochemistry Authors: Juan Tang Yahui Yang Hao-Yan Yin Bin Ma Mengliang Zhu Zi-Shu Yang Xin-Xin Peng Feifei Jia Yao Zhao Fuyi Wang Tianfeng Chen Jun-Long Zhang Source Type: research
Proteomic Profiling of Antimalarial Plasmodione Using 3-Benz(o)ylmenadione Affinity-Based Probes
Chembiochem. 2024 Apr 19:e202400187. doi: 10.1002/cbic.202400187. Online ahead of print.ABSTRACTUnderstanding the mechanisms of drug action in malarial parasites is crucial for the development of new drugs to combat infection and to counteract drug resistance. Proteomics is a widely used approach to study host-pathogen systems and to identify drug protein targets. Plasmodione is an antiplasmodial early-lead drug exerting potent activities against young asexual and sexual blood stages in vitro with low toxicity to host cells. To elucidate its molecular mechanisms, an affinity-based protein profiling (AfBPP) approach was app...
Source: Chembiochem - April 19, 2024 Category: Biochemistry Authors: Ilaria Iacobucci Vittoria Monaco Agn ès Hovasse Baptiste Dupouy Rodrigue Keumoe Bogdan Cichocki Mourad Elhabiri Brigitte Meunier Jean-Marc Strub Maria Monti Sarah Cianf érani St éphanie A Blandin Christine Schaeffer-Reiss Elisabeth Davioud-Charvet Source Type: research
Click, Compute, Create: A Review of Web-based Tools for Enzyme Engineering
Chembiochem. 2024 Apr 18:e202400092. doi: 10.1002/cbic.202400092. Online ahead of print.ABSTRACTEnzyme engineering, though pivotal across various biotechnological domains, is often plagued by its time-consuming and labor-intensive nature. This review aims to offer an overview of supportive in silico methodologies for this demanding endeavor. Starting from methods to predict protein structures, to classification of their activity and even the discovery of new enzymes we continue with describing tools used to increase thermostability and production yields of selected targets. Subsequently, we discuss computational methods to...
Source: Chembiochem - April 18, 2024 Category: Biochemistry Authors: Adrian Tripp Markus Braun Florian Wieser Gustav Oberdorfer Horst Lechner Source Type: research
Click, Compute, Create: A Review of Web-based Tools for Enzyme Engineering
Chembiochem. 2024 Apr 18:e202400092. doi: 10.1002/cbic.202400092. Online ahead of print.ABSTRACTEnzyme engineering, though pivotal across various biotechnological domains, is often plagued by its time-consuming and labor-intensive nature. This review aims to offer an overview of supportive in silico methodologies for this demanding endeavor. Starting from methods to predict protein structures, to classification of their activity and even the discovery of new enzymes we continue with describing tools used to increase thermostability and production yields of selected targets. Subsequently, we discuss computational methods to...
Source: Chembiochem - April 18, 2024 Category: Biochemistry Authors: Adrian Tripp Markus Braun Florian Wieser Gustav Oberdorfer Horst Lechner Source Type: research
Co-Immobilization of ADH and GDH on Metal-organic-framework: An Effective Biocatalyst for Asymmetric Reduction of Ketones
Chembiochem. 2024 Apr 17:e202400147. doi: 10.1002/cbic.202400147. Online ahead of print.ABSTRACTChiral alcohols are not only important building blocks of various bioactive natural compounds and pharmaceuticals, but can serve as synthetic precursors for other valuable organic chemicals, thus the synthesis of these products is of great importance. Bio-catalysis represents one effective way to obtain these molecules, however, the weak stability and high cost of enzymes often hinder its broad application. In this work, we designed a biological nanoreactor by embedding alcohol dehydrogenase (ADH) and glucose dehydrogenase (GDH)...
Source: Chembiochem - April 17, 2024 Category: Biochemistry Authors: Lu Ran Guorong Su Zhenyan Yang Shuke Wu Huailong Teng Source Type: research
Design, Synthesis and Catalytic Activity of Protein Containing Thiotyrosine as an Active Site Residue
Chembiochem. 2024 Apr 17:e202400148. doi: 10.1002/cbic.202400148. Online ahead of print.ABSTRACTNative chemical ligation is a key reaction in the toolbox of chemical methods for the synthesis of native and modified proteins. The catalysis of ligation is commonly performed by using small aryl-thiol molecules added at high concentrations. In this work, we incorporated thiotyrosine, a non-canonical amino acid containing an aryl-thiol moiety, into a designed cyclic protein « sans queue ni tête ». Importantly, the protein environment reduced the pKa of the thiol group to 5.8-5.9, which is significantly lower than the previou...
Source: Chembiochem - April 17, 2024 Category: Biochemistry Authors: Thomas Bachelart Shailesh Kumar Alexis Jouin Mo'ath Yousef Bruno Kieffer Vladimir Torbeev Source Type: research
Using f Örster resonance energy transfer (fret) to understand the ubiquitination landscape
Chembiochem. 2024 Apr 17:e202400193. doi: 10.1002/cbic.202400193. Online ahead of print.ABSTRACTFörster resonance energy transfer (FRET) is a fluorescence technique that allows quantitative measurement of protein interactions, kinetics and dynamics. This review covers the use of FRET to study the structures and mechanisms of ubiquitination and related proteins. We survey FRET assays that have been developed where donor and acceptor fluorophores are placed on E1, E2 or E3 enzymes and ubiquitin (Ub) to monitor steady-state and real-time transfer of Ub through the ubiquitination cascade. Specialized FRET probes placed on Ub ...
Source: Chembiochem - April 17, 2024 Category: Biochemistry Authors: Jashanjot Kaur Gill Gary Shaw Source Type: research
Co-Immobilization of ADH and GDH on Metal-organic-framework: An Effective Biocatalyst for Asymmetric Reduction of Ketones
Chembiochem. 2024 Apr 17:e202400147. doi: 10.1002/cbic.202400147. Online ahead of print.ABSTRACTChiral alcohols are not only important building blocks of various bioactive natural compounds and pharmaceuticals, but can serve as synthetic precursors for other valuable organic chemicals, thus the synthesis of these products is of great importance. Bio-catalysis represents one effective way to obtain these molecules, however, the weak stability and high cost of enzymes often hinder its broad application. In this work, we designed a biological nanoreactor by embedding alcohol dehydrogenase (ADH) and glucose dehydrogenase (GDH)...
Source: Chembiochem - April 17, 2024 Category: Biochemistry Authors: Lu Ran Guorong Su Zhenyan Yang Shuke Wu Huailong Teng Source Type: research
Monomeric far-red and near-infrared fluorescent biliproteins of ultrahigh brightness
We report that fluorescent phycobiliproteins originating from the phycobilisome core subunit ApcF2 can covalently bind biliverdin, named BDFPs. To further improve BDFPs, we conducted a series of studies. Firstly, we mutated K53Q and T144A of BDFPs to increase their effective brightness up to 190% in vivo. Secondly, by homochromatic tandem fusion of high-brightness BDFPs to achieve monomerization, which increases the effective brightness by up to 180% in vivo, and can effectively improve the labeling effect. By combining the above two approaches, the brightness of the tandem BDFPs was much improved compared with that of the...
Source: Chembiochem - April 16, 2024 Category: Biochemistry Authors: Xiang-Xiang Jiang Ya-Nan Hou Li-Wen Lu Kai-Hong Zhao Source Type: research
Gold Nanoparticle Delivery of Glut1 siRNA Facilitates Glucose Starvation Therapy in Lung Cancer
Chembiochem. 2024 Apr 16:e202400239. doi: 10.1002/cbic.202400239. Online ahead of print.ABSTRACTGlucose transporter protein-1 (Glut1), is highly expressed in many cancer types and plays a crucial role in cancer progression through enhanced glucose transport. Its overexpression is associated with aggressive tumor behavior and poor prognosis. Herein, the nucleic acids modified gold nanoparticles (AuNPs) was synthesized to deliver small interfering RNA (siRNA) against Glut1 by microRNA 21 (miR-21) triggers toehold-mediated strand displacement reaction for lung cancer starvation therapy. Overexpression of miR-21 triggers toeho...
Source: Chembiochem - April 16, 2024 Category: Biochemistry Authors: Jiaqi Li Jingyuan Yu Qi Fang Yan Du Xiaojun Zhang Source Type: research
