Fumonisins alone or mixed with other fusariotoxins increase the C22-24:C16 sphingolipid ratios in chicken livers, while deoxynivalenol and zearalenone have no effect
In conclusion the C22-24:C16 ratio emerged as a specific biomarker, with variations dependent only on the presence of fumonisins.PMID:38615975 | DOI:10.1016/j.cbi.2024.111005 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - April 14, 2024 Category: Molecular Biology Authors: Philippe Guerre Elodie Lassallette Ugo Beaujardin-Daurian Angelique Travel Source Type: research
Fumonisins alone or mixed with other fusariotoxins increase the C22-24:C16 sphingolipid ratios in chicken livers, while deoxynivalenol and zearalenone have no effect
In conclusion the C22-24:C16 ratio emerged as a specific biomarker, with variations dependent only on the presence of fumonisins.PMID:38615975 | DOI:10.1016/j.cbi.2024.111005 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - April 14, 2024 Category: Molecular Biology Authors: Philippe Guerre Elodie Lassallette Ugo Beaujardin-Daurian Angelique Travel Source Type: research
Binding Profiles of Human and Mouse Complement Component 8 γ to Trisubstituted Organometallic Compounds
Chem Biol Interact. 2024 Apr 11:110998. doi: 10.1016/j.cbi.2024.110998. Online ahead of print.ABSTRACTComplement component 8gamma (C8γ), a member of the lipocalin protein family, is suggested to act as a carrier protein for various chemicals. Although C8γ has been identified in both humans and rodents for some time, our understanding of the species differences in its chemical binding properties remains limited. In the present study, with the aim to elucidate the potential role of C8γ as a carrier protein in both humans and mice, we conducted a radioligand binding assay to examine the chemical binding properties of human...
Source: Chemico-Biological Interactions - April 13, 2024 Category: Molecular Biology Authors: Katsuya Yamamoto Daisuke Matsumaru Keishi Ishida Satoshi Endo Youhei Hiromori Tsuyoshi Nakanishi Source Type: research
Cationic cycloamylose based nucleic acid nanocarriers
Chem Biol Interact. 2024 Apr 11:111000. doi: 10.1016/j.cbi.2024.111000. Online ahead of print.ABSTRACTNucleic acid delivery by viral and non-viral methods has been a cornerstone for the contemporary gene therapy aimed at correcting the defective genes, replacing of the missing genes, or downregulating the expression of anomalous genes is highly desirable for the management of various diseases. Ostensibly, it becomes paramount for the delivery vectors to intersect the biological barriers for accessing their destined site within the cellular environment. However, the lipophilic nature of biological membranes and their potent...
Source: Chemico-Biological Interactions - April 13, 2024 Category: Molecular Biology Authors: Parteek Prasher Mousmee Sharma Vipul Agarwal Sachin Kumar Singh Gaurav Gupta Harish Dureja Kamal Dua Source Type: research
The E-liquid Flavoring Vanillin Alters Energy and Autophagic Pathways in Human Proximal Tubule (HK-2) Epithelial Cells
In this study, acute exposure to the popular flavoring vanillin (VAN) was performed on human proximal tubule (HK-2) kidney cells. Cells were exposed to 0-1000 μM VAN for 24 or 48 h and cellular stress responses were determined. Mitochondrial viability using MTT assay showed a significant decrease between the control and 1000 μM group by 48 h. Seahorse XFp analysis showed significantly increased basal respiration, ATP production, and proton leak after 24 h exposure. By 48 h exposure, these parameters remained significantly increased in addition to non-mitochondrial respiration and maximal respiration. Glycolytic activity ...
Source: Chemico-Biological Interactions - April 12, 2024 Category: Molecular Biology Authors: Ashley Cox Kathleen C Brown Monica A Valentovic Source Type: research
Bruceantinol works as a CDK2/4/6 inhibitor to inhibit the growth of breast cancer cells
Chem Biol Interact. 2024 Apr 10:110999. doi: 10.1016/j.cbi.2024.110999. Online ahead of print.ABSTRACTBruceantinol (BOL), isolated from the dried fruit of the Brucea javanica (L.) Merr., exhibits cytotoxic effects on breast cancer cells. However, the underlying mechanism remains to be fully addressed. In this paper, the MCF-7 and MDA-MB-231 human breast cancer cell lines were used as experimental models to uncover how BOL inhibits breast cancer cell growth. The effects of BOL on cell growth, proliferation, the cell cycle, and apoptosis were investigated using the MTT assays, EdU incorporation assays, and flow cytometry, re...
Source: Chemico-Biological Interactions - April 12, 2024 Category: Molecular Biology Authors: Li Sun Yumeng Wang Jia Li Shiqing Xu Shuang Xu Jun Li Source Type: research
The E-liquid Flavoring Vanillin Alters Energy and Autophagic Pathways in Human Proximal Tubule (HK-2) Epithelial Cells
In this study, acute exposure to the popular flavoring vanillin (VAN) was performed on human proximal tubule (HK-2) kidney cells. Cells were exposed to 0-1000 μM VAN for 24 or 48 h and cellular stress responses were determined. Mitochondrial viability using MTT assay showed a significant decrease between the control and 1000 μM group by 48 h. Seahorse XFp analysis showed significantly increased basal respiration, ATP production, and proton leak after 24 h exposure. By 48 h exposure, these parameters remained significantly increased in addition to non-mitochondrial respiration and maximal respiration. Glycolytic activity ...
Source: Chemico-Biological Interactions - April 12, 2024 Category: Molecular Biology Authors: Ashley Cox Kathleen C Brown Monica A Valentovic Source Type: research
Bruceantinol works as a CDK2/4/6 inhibitor to inhibit the growth of breast cancer cells
Chem Biol Interact. 2024 Apr 10:110999. doi: 10.1016/j.cbi.2024.110999. Online ahead of print.ABSTRACTBruceantinol (BOL), isolated from the dried fruit of the Brucea javanica (L.) Merr., exhibits cytotoxic effects on breast cancer cells. However, the underlying mechanism remains to be fully addressed. In this paper, the MCF-7 and MDA-MB-231 human breast cancer cell lines were used as experimental models to uncover how BOL inhibits breast cancer cell growth. The effects of BOL on cell growth, proliferation, the cell cycle, and apoptosis were investigated using the MTT assays, EdU incorporation assays, and flow cytometry, re...
Source: Chemico-Biological Interactions - April 12, 2024 Category: Molecular Biology Authors: Li Sun Yumeng Wang Jia Li Shiqing Xu Shuang Xu Jun Li Source Type: research
Biochemical, structural, and computational analyses of two new clinically identified missense mutations of ALDH7A1
We report a biochemical, structural, and computational study of two novel ALDH7A1 missense mutations that were identified in a child with rare recurrent seizures from the third month of life. The mutations affect two residues in the oligomer interfaces of ALDH7A1, Arg134 and Arg441 (Arg162 and Arg469 in the HGVS nomenclature). The corresponding enzyme variants R134S and R441C (p.Arg162Ser and p.Arg469Cys in the HGVS nomenclature) were expressed in Escherichia coli and purified. R134S and R441C have 10,000- and 50-fold lower catalytic efficiency than wild-type ALDH7A1, respectively. Sedimentation velocity analytical ultrace...
Source: Chemico-Biological Interactions - April 11, 2024 Category: Molecular Biology Authors: David A Korasick David P Buckley Alessandra Palpacelli Ida Cursio Elisabetta Cesaroni Jianlin Cheng John J Tanner Source Type: research
The Impact of Formaldehyde Exposure on Lung Inflammatory Disorders: Insights into Asthma, Bronchitis, and Pulmonary Fibrosis
Chem Biol Interact. 2024 Apr 9:111002. doi: 10.1016/j.cbi.2024.111002. Online ahead of print.ABSTRACTLung inflammatory disorders are a major global health burden, impacting millions of people and raising rates of morbidity and death across many demographic groups. An industrial chemical and common environmental contaminant, formaldehyde (FA) presents serious health concerns to the respiratory system, including the onset and aggravation of lung inflammatory disorders. Epidemiological studies have shown significant associations between FA exposure levels and the incidence and severity of several respiratory diseases. FA caus...
Source: Chemico-Biological Interactions - April 11, 2024 Category: Molecular Biology Authors: Asif Ahmad Bhat Muhammad Afzal Ahsas Goyal Gaurav Gupta Riya Thapa Waleed Hassan Almalki Imran Kazmi Sami I Alzarea Moyad Shahwan Keshav Raj Paudel Haider Ali Deepak Sahu Parteek Prasher Sachin Kumar Singh Kamal Dua Source Type: research
Biochemical, structural, and computational analyses of two new clinically identified missense mutations of ALDH7A1
We report a biochemical, structural, and computational study of two novel ALDH7A1 missense mutations that were identified in a child with rare recurrent seizures from the third month of life. The mutations affect two residues in the oligomer interfaces of ALDH7A1, Arg134 and Arg441 (Arg162 and Arg469 in the HGVS nomenclature). The corresponding enzyme variants R134S and R441C (p.Arg162Ser and p.Arg469Cys in the HGVS nomenclature) were expressed in Escherichia coli and purified. R134S and R441C have 10,000- and 50-fold lower catalytic efficiency than wild-type ALDH7A1, respectively. Sedimentation velocity analytical ultrace...
Source: Chemico-Biological Interactions - April 11, 2024 Category: Molecular Biology Authors: David A Korasick David P Buckley Alessandra Palpacelli Ida Cursio Elisabetta Cesaroni Jianlin Cheng John J Tanner Source Type: research
The Impact of Formaldehyde Exposure on Lung Inflammatory Disorders: Insights into Asthma, Bronchitis, and Pulmonary Fibrosis
Chem Biol Interact. 2024 Apr 9:111002. doi: 10.1016/j.cbi.2024.111002. Online ahead of print.ABSTRACTLung inflammatory disorders are a major global health burden, impacting millions of people and raising rates of morbidity and death across many demographic groups. An industrial chemical and common environmental contaminant, formaldehyde (FA) presents serious health concerns to the respiratory system, including the onset and aggravation of lung inflammatory disorders. Epidemiological studies have shown significant associations between FA exposure levels and the incidence and severity of several respiratory diseases. FA caus...
Source: Chemico-Biological Interactions - April 11, 2024 Category: Molecular Biology Authors: Asif Ahmad Bhat Muhammad Afzal Ahsas Goyal Gaurav Gupta Riya Thapa Waleed Hassan Almalki Imran Kazmi Sami I Alzarea Moyad Shahwan Keshav Raj Paudel Haider Ali Deepak Sahu Parteek Prasher Sachin Kumar Singh Kamal Dua Source Type: research
Reduced light exposure mitigates streptozotocin-induced vascular changes and gliosis in diabetic retina by an anti-inflammatory effect and increased retinal cholesterol turnover
Chem Biol Interact. 2024 Apr 7:110996. doi: 10.1016/j.cbi.2024.110996. Online ahead of print.ABSTRACTDiabetic retinopathy is not cured efficiently and changes of lifestyle measures may delay early retinal injury in diabetes. The aim of our study was to investigate the effects of reduced daily light exposure on retinal vascular changes in streptozotocin (STZ)-induced model of DM with emphasis on inflammation, Aqp4 expression, visual cycle and cholesterol metabolism-related gene expression in rat retina and RPE. Male Wistar rats were divided into the following groups: 1. control; 2. diabetic group (DM) treated with streptozo...
Source: Chemico-Biological Interactions - April 9, 2024 Category: Molecular Biology Authors: Dolika D Vasovi ć Sanja Ivkovi ć Ana Živanović Tamara Major Jelena M Mila šin Na đa S Nikolić Jelena M Simonovi ć Nikola Šutulović Dragan Hrn čić Olivera Stanojlovi ć Milena Veskovi ć Dejan M Ra šić Du šan Mladenović Source Type: research
Reduced light exposure mitigates streptozotocin-induced vascular changes and gliosis in diabetic retina by an anti-inflammatory effect and increased retinal cholesterol turnover
Chem Biol Interact. 2024 Apr 7:110996. doi: 10.1016/j.cbi.2024.110996. Online ahead of print.ABSTRACTDiabetic retinopathy is not cured efficiently and changes of lifestyle measures may delay early retinal injury in diabetes. The aim of our study was to investigate the effects of reduced daily light exposure on retinal vascular changes in streptozotocin (STZ)-induced model of DM with emphasis on inflammation, Aqp4 expression, visual cycle and cholesterol metabolism-related gene expression in rat retina and RPE. Male Wistar rats were divided into the following groups: 1. control; 2. diabetic group (DM) treated with streptozo...
Source: Chemico-Biological Interactions - April 9, 2024 Category: Molecular Biology Authors: Dolika D Vasovi ć Sanja Ivkovi ć Ana Živanović Tamara Major Jelena M Mila šin Na đa S Nikolić Jelena M Simonovi ć Nikola Šutulović Dragan Hrn čić Olivera Stanojlovi ć Milena Veskovi ć Dejan M Ra šić Du šan Mladenović Source Type: research
Accumulated LPS induced by colitis altered the activities of vitamin D-metabolizing hydroxylases and decreased the generation of 25-hydroxyvitamin D
Chem Biol Interact. 2024 Apr 6:110997. doi: 10.1016/j.cbi.2024.110997. Online ahead of print.ABSTRACTIt is generally accepted that low vitamin D (VD) levels are associated with a high prevalence factor for Inflammatory bowel disease(IBD). IBD patients have observed higher levels of lipopolysaccharide(LPS), ALT, and AST than healthy people. Gut-derived LPS causes inflammatory injury in the liver and kidney. The VD-metabolizing mechanism is involved in the liver and kidney, which means IBD might impact VD metabolism. However, whether IBD affects VD metabolism has not been studied. In vitro LPS resulted in decreased CYP2R1 in...
Source: Chemico-Biological Interactions - April 8, 2024 Category: Molecular Biology Authors: Yingyu Lu Hao Chen Yonger Chen Liang Zhao Shaozhen Hou Source Type: research
