Clinical Microbiology and Infection
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1028 records returned
Personalized management of patients with inoperable alveolar echinococcosis undergoing treatment with albendazole: usefulness of positron-emission-tomography combined with serological and computed tomography follow-up
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Clin Microbiol Infect The present study aimed to identify a sub-group of inoperable alveolar echinococcosis (AE) patients undergoing long-term treatment with benzimidazole (BZM) who presented with an evolution suggestive of a parasitocidal effect. An evolution compatible with parasite death was observed in five patients. (Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - November 14, 2009 Category: Microbiology Authors: J. Crouzet, F. Grenouillet, E. Delabrousse, O. Blagosklonov, T. Thevenot, V. Di Martino, R. Piarroux, G. A. Mantion, S. Bresson-Hadni Source Type: journals
Clinical, molecular and epidemiological description of a cluster of community-associated methicillin-resistant Staphylococcus aureus isolates from injecting drug users with bacteraemia
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Clin Microbiol Infect Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is an increasing problem, predominantly in previously healthy individuals including notable risk groups such as the homeless, those who play close-contact sports, military personnel, men who have sex with men (MSM) and injecting drug users (IDUs). Over a 5-month period, four IDUs were admitted to Addenbrooke's Hospital, Cambridge, UK, with MRSA bacteraemia. All four patients presented with complex clinical features, with more than one focus of infection, and were linked epidemiologically. The atypical antibiogram of the MRSA is...
Source: Clinical Microbiology and Infection - November 12, 2009 Category: Microbiology Authors: F. J. Cooke, E. Gkrania-Klotsas, J. C. Howard, M. Stone, A. M. Kearns, M. Ganner, A. J. Carmichael, N. M. Brown Source Type: journals
A variant of the Southern German clone of methicillin-resistant Staphylococcus aureus is predominant in Croatia
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Clin Microbiol Infect The aim of the present study was to investigate the antibiotic susceptibility patterns and molecular epidemiology of clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered in 24 hospitals in 20 cities in Croatia from October to December 2004. A total of 1815 consecutive S. aureus isolates were recovered, 248 of which were MRSA. The MRSA isolates were analysed using spa typing, multilocus sequence typing and SCCmec typing. Furthermore, the presence of Panton[ndash]Valentine leukocidin (PVL) genes was determined as a genetic marker for community-associated MRSA. The MRSA prevalen...
Source: Clinical Microbiology and Infection - November 11, 2009 Category: Microbiology Authors: A. Budimir, R. H. Deurenberg, Z. Bo[scaron]njak, E. E. Stobberingh, H. Cetkovic, S. Kalenic Source Type: journals
Validity of interferon-γ-release assays for the diagnosis of latent tuberculosis in haemodialysis patients
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In conclusion, the IGRAs can be useful for the diagnosis of latent TB infection in haemodialysis patients. (Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - November 10, 2009 Category: Microbiology Authors: W. K. Chung, Z. L. Zheng, J. Y. Sung, S. Kim, H. H. Lee, S. J. Choi, J. Yang Source Type: journals
Characterization of two novel gene cassettes, dfrA27 and aadA16, in a non-O1, non-O139 Vibrio cholerae isolate from China
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Clin Microbiol Infect Two novel integron-borne cassettes, dfrA27 (encoding trimethoprim resistance) and aadA16 (encoding streptomycin and spectinomycin resistance), located on a conjugative plasmid, have been found in a non-O1, non-O139 Vibrio cholera isolate. DfrA27 shares 75% amino acid identity with DfrA5, and AadA16 shares 88% identity with AdaA6. Cloning of the cassette region and expression analysis demonstrated that the aadA16 gene can be expressed from its own promoter sequence, which is present upstream of the aadA16 cassette. (Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - November 10, 2009 Category: Microbiology Authors: J. Sun, M. Zhou, Q. Wu, Y. Ni Source Type: journals
Tick-borne encephalitis (TBE) virus prevalence and virus genome characterization in field-collected ticks (Ixodes ricinus) from risk, non-risk and former risk areas of TBE, and in ticks removed from humans in Germany
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In this study, ticks were collected in Germany from two non-risk areas in the states of Saxony-Anhalt and Mecklenburg-Western Pomerania, where several single human TBE cases have occurred in recent years. Ticks were also collected from a region in Thuringia, known to be a former risk area for TBE virus (TBEV), where numerous human cases were reported between 1960 and 1975. Detection of TBEV RNA was conducted by real-time RT-PCR. No TBEV was detected in any field-collected ticks. However, ticks were also collected from volunteers living in Bavaria. Three of 239 ticks from this collection were positive for TBEV genome and tw...
Source: Clinical Microbiology and Infection - November 10, 2009 Category: Microbiology Authors: C. Klaus, B. Hoffmann, U. Hering, B. Mielke, K. Sachse, M. Beer, J. Süss Source Type: journals
Pulmonary aspergillosis in patients with chronic obstructive pulmonary disease: incidence, risk factors, and outcome
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We describe a large series of patients with chronic obstructive pulmonary disease (COPD) and probable invasive pulmonary aspergillosis (IPA), and the risk factors and incidence of the disease in patients with isolation of Aspergillus from lower respiratory tract samples. From 2000 to 2007, we retrospectively studied all patients admitted with COPD and isolation of Aspergillus (239; 16.3/1000 admissions). Multivariate logistic regression and survival curves were used. Fifty-three patients had probable IPA (3.6 cases of IPA per 1000 COPD admissions). IPA affects at least 22.1% of patients with COPD and isolation of Aspergill...
Source: Clinical Microbiology and Infection - November 10, 2009 Category: Microbiology Authors: J. Guinea, M. Torres-Narbona, P. Gijón, P. Muñoz, F. Pozo, T. Peláez, J. de Miguel, E. Bouza Source Type: journals
Extracellular adherence protein (Eap) from Staphylococcus aureus does not function as a superantigen
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In this study, we focused on whether Eap fulfilled the definition criteria for a superantigen. We demonstrate that T-cell activation by Eap is dependent on both major histocompatibility complex class II and intercellular adhesion molecule type 1, that cellular processing is required for Eap to elicit T-cell proliferation, and that the kinetics of proliferation resemble the profile of a conventional antigen and not that of a superantigen. (Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - November 10, 2009 Category: Microbiology Authors: A. Haggar, J.-I. Flock, A. Norrby-Teglund Source Type: journals
The prevalence of middle ear pathogens in the outer ear canal and the nasopharyngeal cavity of healthy young adults
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Clin Microbiol Infect Culturing middle ear fluid samples from children with chronic otitis media with effusion (OME) using standard techniques results in the isolation of bacterial species in approximately 30[ndash]50% of the cases. Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis, the classic middle ear pathogens of acute otitis media, are involved but, recently, several studies suggested Alloiococcus otitidis as an additional pathogen. In the present study, we used species-specific PCRs to establish the prevalence, in both the nasopharyngeal cavity and the outer ear, of H. influenzae, M. catarrh...
Source: Clinical Microbiology and Infection - November 6, 2009 Category: Microbiology Authors: T. De Baere, M. Vaneechoutte, P. Deschaght, J. Huyghe, I. Dhooge Source Type: journals
Determinants of Moraxella catarrhalis colonization in healthy Dutch children during the first 14 months of life
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This study investigated a target cohort group of 1079 children forming part of the Generation R Study, a population-based prospective cohort study following children from fetal life until young adulthood, conducted in Rotterdam, The Netherlands. Nasopharyngeal swabs for M. catarrhalis culture were obtained at 1.5, 6 and 14 months of age, with all three swabs being available for analyses from 443 children. Data on risk factors possibly associated with M. catarrhalis colonization were obtained by questionnaire at 2, 6 and 12 months. M. catarrhalis colonization increased from 11.8% at age 1.5 months to 29.9% and 29.7% at 6 an...
Source: Clinical Microbiology and Infection - November 6, 2009 Category: Microbiology Authors: S. J. C. Verhaegh, A. Lebon, J. A. Saarloos, H. A. Verbrugh, V. W. V. Jaddoe, A. Hofman, J. P. Hays, H. A. Moll, A. van Belkum Source Type: journals
Haemophilus influenzae serotype b conjugate vaccine failure in twelve countries with established national childhood immunization programmes
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Clin Microbiol Infect The present study describes the clinical and immunological features of children with Hib vaccine failure, who were identified through national surveillance between 1996 and 2001 in Europe, Israel and Australia. True vaccine failure was defined as invasive Hib disease occurring [ge]2 weeks after one dose, given after the first birthday, or [ge]1 week after [ge]2 doses, given at (Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - November 5, 2009 Category: Microbiology Authors: S. Ladhani, P. T. Heath, M. P. E. Slack, P. B. McIntyre, J. Diez-Domingo, J. Campos, R. Dagan, M. E. Ramsay Source Type: journals
MRSA screening: can one swab be used for both culture and rapid testing?An evaluation of chromogenic culture and subsequent Hain GenoQuick PCR amplification/detection
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Clin Microbiol Infect The use of a single swab for both MRSA culture and for rapid testing by PCR was evaluated, using the Hain GenoQuick (GQM) methicillin resistant Staphylococcus aureus (MRSA) assay for the rapid detection of MRSA, as a single swab would be the preferred option for routine diagnostic testing. GQM detected current prevalent Irish MRSA strains incorporating all known SSCmec types, including Panton[ndash]Valentine leukocidin-positive strains. Using the GQM method, all methicillin-resistant coagulase-negative staphylococci tested were confirmed to be negative, although three of seven gentamicin-resistant met...
Source: Clinical Microbiology and Infection - November 4, 2009 Category: Microbiology Authors: O. Sherlock, A. Dolan, H. Humphreys Source Type: journals
Who are the carriers of MRSA in the community? A prospective study in the Pays de la Loire region of France
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Clin Microbiol Infect The aim of this study was to determine the demographic characteristics of methicillin-resistant Staphylococcus aureus (MRSA) carriers in the community, to assess their risk factors and possible past hospitalization history and to describe the different resistance phenotypes of community isolates of S. aureus. Data were collected over the course of 16 months (from June 2005 to September 2006) in the Pays de la Loire region of France by MedQual, a network of private biological analysis laboratories. This work was based solely on the analysis of strains isolated in the community as opposed to isolates fr...
Source: Clinical Microbiology and Infection - November 4, 2009 Category: Microbiology Authors: S. Thibaut, J. Caillon, D. Lepelletier, P. Lombrail, G. Potel, F. Ballereau Source Type: journals
Effect of surface roughness and sterilization on bacterial adherence to ultra-high molecular weight polyethylene
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Clin Microbiol Infect Sterilization with ethylene oxide (EO) and gas plasma (GP) are well-known methods applied to ultra-high molecular weight polyethylene (UHMWPE) surfaces in the belief that they prevent major material changes caused by gamma irradiation. However, the influence of these surface sterilization methods on bacterial adherence to UHMWPE is unknown. UHMWPE samples with various degrees of roughness (0.3, 0.8 and 2.0 [mu]m) were sterilized with either GP or EO. The variations in hydrophobicity, surface free energy and surface functional groups were investigated before and after sterilization. Sterilized samples ...
Source: Clinical Microbiology and Infection - November 4, 2009 Category: Microbiology Authors: T. J. Kinnari, J. Esteban, N. Zamora, R. Fernandez, C. López-Santos, F. Yubero, D. Mariscal, J. A. Puertolas, E. Gomez-Barrena Source Type: journals
A possible novel Francisella genomic species isolated from blood and urine of a patient with severe illness
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Clin Microbiol Infect Two identical isolates were recovered in pure culture from the blood and urine of a patient suffering from severe septicaemia associated with obstructive pyelonephritis secondary to lithotripsy. Preliminary phenotypic and genotypic characterizations based on serological, biochemical and sequence analyses following PCR amplification of selected gene regions indicate that this organism represents a potential new Francisella genomic species. (Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - October 30, 2009 Category: Microbiology Authors: R. Escudero, M. Elía, J. A. Sáez-Nieto, V. Menéndez, A. Toledo, G. Royo, M. Rodríguez-Vargas, M. J. Whipp, H. Gil, I. Jado, P. Anda Source Type: journals
Plasmid-mediated QnrS2 determinant in an Aeromonas caviae isolate recovered from a patient with diarrhoea
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Clin Microbiol Infect A qnrS2 gene was identified in an Aeromonas caviae isolate (MICs of ciprofloxacin, norfloxacin and ofloxacin >32 mg/L) from a stool sample collected from a patient with gastroenteritis. The analysis of the gyrA and parC genes revealed amino acid substitutions Ser83-Ile and Ser80-Thr, respectively. In addition, five out of 41 nalidixic acid-resistant Aeromonas isolates studied (26 identified as Aeromonas veronii bv sobria and 15 identified as A. caviae) showed ciprofloxacin resistance. The identification of plasmid-mediated qnr genes outside of the Enterobacteriaceae underlines a possible diffusion of ...
Source: Clinical Microbiology and Infection - October 28, 2009 Category: Microbiology Authors: A. Arias, C. Seral, F. Navarro, E. Miró, P. Coll, F. J. Castillo Source Type: journals
Clinical and microbiological characteristics of Nocardiosis including those caused by emerging Nocardia species in Taiwan, 1998–2008
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In conclusion, the clinical and microbiological manifestations of Nocardiosis vary with the different Nocardia species. Accurate identification of the species is crucial to make the diagnosis. (Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - October 22, 2009 Category: Microbiology Authors: C.-K. Tan, C.-C. Lai, S.-H. Lin, C.-H. Liao, C.-H. Chou, H.-L. Hsu, Y.-T. Huang, P.-R. Hsueh Source Type: journals
Diminished in vitro antibacterial activity of oxacillin against clinical isolates of borderline oxacillin-resistant Staphylococcus aureus
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In conclusion, oxacillin was non-effective in the eradication of either (i) BORSA with oxacillin MICs [ge]4 mg/L or (ii) [beta]-lactamase-hyperproducing BORSA (MICs [le]2 mg/L). Further investigation into [beta]-lactam dosing strategies against different BORSA strains is warranted in order to avoid possible therapy failure. (Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - October 21, 2009 Category: Microbiology Authors: S. Croes, P. S. Beisser, P. H. Terporten, C. Neef, R. H. Deurenberg, E. E. Stobberingh Source Type: journals
Intrathecal colistin for drug-resistant Acinetobacter baumannii central nervous system infection: a case series and systematic review
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Clin Microbiol Infect Treatment limitations exist for drug-resistant Acinetobacter baumannii central nervous system (CNS) infection. We conducted a retrospective study and systematic literature review to identify patients with drug-resistant A. baumannii CNS infection who received primary or adjunct intrathecal or intraventricular (IT/IVT) colistin. In a case series of seven Thai patients and 17 patients identified in the literature, clinical and microbiological cure rates with IT/IVT colistin therapy were 83% and 92%, respectively. Three patients (13%) developed chemical ventriculitis and one (4%) experienced treatment-as...
Source: Clinical Microbiology and Infection - October 20, 2009 Category: Microbiology Authors: T. Khawcharoenporn, A. Apisarnthanarak, L. M. Mundy Source Type: journals
Emergence of Crimean–Congo haemorrhagic fever in Greece
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Clin Microbiol Infect In the summer of 2008, the first case of Crimean[ndash]Congo haemorrhagic fever (CCHF) was observed in Greece. The laboratory diagnosis was established using nested RT-PCR and quantitative real-time RT-PCR. A high viral load and increased levels of cytokines were detected on the third day of illness and the patient died 7 days after the onset of symptoms. Nucleotide sequence analysis revealed that the Greek CCHF virus strain had high sequence identity with other Balkan CCHF virus strains. (Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - October 19, 2009 Category: Microbiology Authors: A. Papa, V. Dalla, E. Papadimitriou, G. N. Kartalis, A. Antoniadis Source Type: journals
Voriconazole concentrations and outcome of invasive fungal infections
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Clin Microbiol Infect Twenty-five patients with proven or probable invasive fungal infections (IFIs) who experienced two or more episodes of voriconazole therapeutic drug monitoring (TDM) at a tertiary referral hospital were reviewed to explore the association between serum trough concentrations and outcomes of IFI. Microbiological and/or clinical success, in addition to IFI-related mortality, was assessed. We performed two separate analyses, one based on the initial trough voriconazole concentration at steady state, and the other on the median trough voriconazole concentration (derived from repeated TDM episodes) for each...
Source: Clinical Microbiology and Infection - October 19, 2009 Category: Microbiology Authors: S. Miyakis, S. J. van Hal, J. Ray, D. Marriott Source Type: journals
Disseminated disease caused by Mycobacterium simiae in AIDS patients: a report of three cases
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We report on three AIDS patients who were found to suffer from mycobacteraemia caused by M. simiae in a rural tertiary-care hospital in central India. (Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - October 15, 2009 Category: Microbiology Authors: R. Narang, P. Narang, A. P. Jain, D. K. Mendiratta, R. Joshi, M. Lavania, R. Das, V. M. Katoch Source Type: journals
Comparison of three real-time PCR methods with blood smears and rapid diagnostic test in Plasmodium sp. infection
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In this study three different quantitative, real-time PCR methods were compared with routine methods used for malaria diagnosis. A comparative study was conducted prospectively in the laboratories of Montpellier and Nîmes University Hospitals. The methods used for routine diagnostic malaria testing consisted of microscopic examination of Giemsa-stained blood smears and rapid diagnostic tests. Three quantitative real-time PCR methods (qRT-PCR) were tested: qRT-PCR1 amplified a specific sequence on the P. falciparum Cox1 gene, qRT-PCR2 amplified a species-specific region of the multicopy 18S rDNA, and qRT-PCR3 amplified a m...
Source: Clinical Microbiology and Infection - October 14, 2009 Category: Microbiology Authors: N. Bourgeois, A. Boutet, P-J. Bousquet, D. Basset, C. Douard-Enault, S. Charachon, L. Lachaud Source Type: journals
New molecular method for the detection of human papillomavirus type 16 integration
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Clin Microbiol Infect Human papillomavirus (HPV) infection is the cause of cervical cancer. Integration of HPV-16 DNA in cervical cells is considered to be a key event in the progression towards invasive cancer, but little is known about this event in anal carcinogenesis. The integration could be a useful biomarker for cancer progression. Optimized assays are needed to determine the value of real-time detection of HPV integration in longitudinal studies, and this approach is only possible with a high-throughput assay. The aim of this study was to develop a new multiplex real-time PCR assay based on simultaneous amplificati...
Source: Clinical Microbiology and Infection - October 14, 2009 Category: Microbiology Authors: M. P. Cañadas, L. Darwich, G. Sirera, V. Cirigliano, M. Bofill, B. Clotet, S. Videla Source Type: journals
Comparison of Rickettsia conorii growth within different cell lines by real-time quantitative PCR
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - October 14, 2009 Category: Microbiology Authors: P. Balraj, G. Vestris, D. Raoult, P. Renesto Source Type: journals
Spotted fever rickettsioses in children in Sri Lanka
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 28, 2009 Category: Microbiology Authors: K. Nagalingam, J.M. Rolain, V. Thevanesam, F. LakKumar, G. Gunawardana, D. Raoult Source Type: journals
Rickettsiae in ticks from wild ungulates of Sierra Nevada and Doñana national parks (Spain)
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: F. J. Márquez Source Type: journals
Emergence of Rickettsia slovaca infection in Brittany, France
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: F. Méchaï, M. Revest, F. Lanternier, J. M. Rolain, J. P. Viard, O. Lortholary, M. Lecuit Source Type: journals
Seroprevalence of Q-fever, brucellosis and leptospirosis in farmers and agricultural workers in Bari, southern Italy
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: R. Monno, L. Fumarola, P. Trerotoli, D. Cavone, T. Massaro, L. Spinelli, C. Rizzo, M. Musti Source Type: journals
Pathogen prevalence and blood meal identification in Amblyomma ticks as a means of reservoir host determination for ehrlichial pathogens
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: K. A. Pierce, C. D. Paddock, J. W. Sumner, W. L. Nicholson Source Type: journals
Canine ehrlichiosis in the Ivory Coast and Gabon: alteration of biochemical blood parameters based on Ehrlichia canis serology
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: C. Roqueplo, V. Cheminel, O. Bourry, J. Gomez, J. -M. Prévosto, D. Parzy, B. Davoust Source Type: journals
Molecular detection of Bartonella quintana in human body lice from Mexico City
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: V. Alcantara, J.-M. Rolain, A. G. Eduardo, M. J. Raul, D. Raoult Source Type: journals
Anaplasma phagocytophilum and Ehrlichia muris induce cytopenias and global defects in hematopoiesis
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: D. Borjesson, K. MacNamara, J. Johns, G. Winslow Source Type: journals
Comparison of the performances of MLVA vs. the main other typing techniques for Bartonella henselae
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: R. Bouchouicha, H.-J. Boulouis, M. Berrich, M. Monteil, B. Chomel, N. Haddad Source Type: journals
Prevalence of Coxiella burnetii and Bartonella species as cases of infective endocarditis in Marseilles (1994–2007)
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: J.-P. Casalta, F. Gouriet, H. Richet, F. Thuny, G. Habib, D. Raoult Source Type: journals
Detection of Bartonella henselae – DNA in macronodular hepatic lesions of an immunocompetent woman
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: S. Mastrandrea, M. Simonetta Taras, P. Capitta, S. Tola, V. Marras, G. Strusi, G. Masala Source Type: journals
Seroprevalence survey of equine anaplasmosis in France and in sub-Saharan Africa
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: L. Maurizi, J.-L. Marié, C. Courtin, S. Gorsane, D. Chal, B. Davoust Source Type: journals
Seroprevalence of Q-fever in Algeria
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: A. Lacheheb, D. Raoult Source Type: journals
Evidence of horizontal gene transfer between amoeba and bacteria
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: C. Moliner, D. Raoult, P.-E. Fournier Source Type: journals
Efficiency of a phase 1 vaccine for the reduction of vaginal Coxiella burnetii shedding in a clinically affected goat herd
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: E. Rousset, B. Durand, J. L. Champion, M. Prigent, P. Dufour, C. Forfait, M. Marois, T. Gasnier, V. Duquesne, R. Thiéry, M. F. Aubert Source Type: journals
Characterisation of the Omsk collection of rickettsial strains
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: N. V. Rudakov, S. N. Schpynov, I. E. Samoylenko, P.-E. Fournier, T. A. Reschetnikova, L. V. Kumpan, D. Raoult Source Type: journals
Characterisation of Orientia tsutsugamushi genotypes from wild rodents and chigger mites in Korea
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: S.-K. Shim, E.-N. Choi, K.-O. Yu, H.-J. Park, C.-M. Kim, K.-H. Lee, J.-K. Park, P.-H. Park, M.-H. Yoon, S.-H. Park, Y.-S. Choi, K.-J. Hwang, M.-Y. Park Source Type: journals
Prevalence of infection with Rickettsia helvetica in feeding ticks and their hosts in western Poland
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(Source: Clinical Microbiology and Infection)
Source: Clinical Microbiology and Infection - September 27, 2009 Category: Microbiology Authors: J. Stańczak, M. Racewicz, J. Michalik, S. Cieniuch, B. Sikora, M. Skoracki Source Type: journals
Comparison of antifungal MICs for yeasts obtained using the EUCAST method in a reference laboratory and the Etest in nine different hospital laboratories
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Clin Microbiol Infect In routine laboratory practice, the determination of MICs of antifungals for yeasts often relies on the Etest, because of a good correlation with reference methods. However, this correlation was established through predesigned studies, rather than prospective testing. The surveillance programme of fungaemia (YEASTS programme), implemented since 2003, facilitated our comparison of the Etest and the EUCAST results, obtained on a routine basis in nine different hospitals and in a reference laboratory, respectively. The analysis included 690 isolates recovered from blood culture (362 Candida albicans, 113...
Source: Clinical Microbiology and Infection - September 23, 2009 Category: Microbiology Authors: E. Dannaoui, A. Paugam, M. Develoux, C. Chochillon, J. Matheron, A. Datry, C. Bouges-Michel, C. Bonnal, F. Dromer, S. Bretagne Source Type: journals
A serosurvey of Orientia tsutsugamushi from patients with scrub typhus
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Clin Microbiol Infect Many countries where scrub typhus is endemic use their own cutoff values for antibody titres to differentiate between cured cases and current infections. To establish an antibody titre cutoff value, one needs to investigate the seroprevalence in endemic areas, and the duration of the increase in titre after complete cure. We conducted a follow-up study of anti-Orientia tsutsugamushi antibody titres using indirect immunofluorescence assays (IFA) and passive haemagglutination assays (PHA) in patients with scrub typhus. After the onset of symptoms, IgM antibody titres increased gradually over 2[ndash]3 w...
Source: Clinical Microbiology and Infection - September 22, 2009 Category: Microbiology Authors: D. M. Kim, Y.-M. Lee, J.-H. Back, T. Y. Yang, J. H. Lee, H.-J. Song, S.-K. Shim, K.-J. Hwang, M.-Y. Park Source Type: journals
Pregnancy and Crimean-Congo haemorrhagic fever
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Clin Microbiol Infect Crimean-Congo Hemorrhagic fever (CCHF) is a potentially fatal viral infection with reported case fatality rates of 5[ndash]30%. Humans become infected through tick bites, by contact with a patient with CCHF during the acute phase of infection, or by contact with blood or tissues from viraemic livestock. In this first report in the literature, we present the characteristics of three pregnant women with CCHF infection and the outcome of their babies. Transmission of the CCHF infection could be either intrauterine or perinatal. In endemic regions, CCHF infection should be considered in the differential d...
Source: Clinical Microbiology and Infection - September 22, 2009 Category: Microbiology Authors: O. Ergonul, A. Celikbas, U. Yildirim, A. Zenciroglu, D. Erdogan, I. Ziraman, F. Saracoglu, N. Demirel, O. Cakmak, B. Dokuzoguz Source Type: journals
Performance of VITEK-2 Compact and overnight MicroScan panels for direct identification and susceptibility testing of Gram-negative bacilli from positive FAN BacT/ALERT blood culture bottles
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We describe the reliability of the VITEK-2 Compact and overnight MicroScan panels for direct identification and susceptibility testing from the BacT/ALERT blood culture system when using FAN (FA and FN) bottles. A simple procedure, in two centrifugation steps, was designed to remove the charcoal particles present in FA and FN bottles. A total of 113 positive blood cultures showing Gram-negative rods were investigated. Enterobacteriaceae were isolated in 104 cases, and Pseudomonas aeruginosa in nine. The MicroScan system correctly identified 106 (93.8%) of the 113 isolates. The seven identificaction errors included P. aerug...
Source: Clinical Microbiology and Infection - September 22, 2009 Category: Microbiology Authors: M. D. Quesada, M. Giménez, S. Molinos, G. Fernández, M. D. Sánchez, R. Rivelo, A. Ramírez, G. Banqué, V. Ausina Source Type: journals
Leptospirosis pulmonary haemorrhage syndrome is associated with linear deposition of immunoglobulin and complement on the alveolar surface
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Clin Microbiol Infect Leptospirosis is a zoonotic infection associated with severe diseases such as leptospirosis pulmonary haemorrhage syndrome (LPHS). The cause of pulmonary haemorrhage is unclear. Understanding which mechanisms and processes are involved in LPHS will be important in treatment regimens under development for this life-threatening syndrome. In the present study, we evaluated 30 lung specimens from LPHS patients and seven controls using histology and immunohistochemistry (detection of IgM, IgG, IgA and C3) in order to describe the pathological features associated with this syndrome. Immunoglobulin deposits ...
Source: Clinical Microbiology and Infection - September 22, 2009 Category: Microbiology Authors: J. Croda, A. N. D. Neto, R. A. Brasil, C. Pagliari, A. C. Nicodemo, M. I. S. Duarte Source Type: journals
Easily available adjustment criteria for the comparison of antibiotic consumption in a hospital setting: experience in France
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This study investigated the relationship between AbC expressed as defined daily doses per 1000 patient-days (DDD/1000 PDs) or per 100 admissions (DDD/100 admissions) and the number of venous central lines, the number of episodes of bacteraemia and various hospital characteristics. The relationship was tested using multiple linear analyses. The median total AbC in public hospitals was 395 DDD/1000 PDs (range, 196[ndash]737) and 341 DDD/100 admissions (range, 180[ndash]792). In private hospitals this was 422 DDD/1000 PDs (range, 113[ndash]717) and 212 DDD/100 admissions (range, 38[ndash]510). The best model for public hospit...
Source: Clinical Microbiology and Infection - September 22, 2009 Category: Microbiology Authors: B. Amadeo, C. Dumartin, P. Robinson, A. G. Venier, P. Parneix, J. P. Gachie, A. Fourrier-Réglat, A. M. Rogues Source Type: journals
Circulation of international clones of levofloxacin non-susceptible Streptococcus pneumoniae in Taiwan
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Clin Microbiol Infect Levofloxacin susceptibility testing was carried out for a total of 2539 Streptococcus pneumoniae isolates obtained from January 2001 to February 2008 at the National Taiwan University Hospital (NTUH) and a further 228 pneumococcal isolates obtained from January 2004 to December 2006 at three other hospitals in different geographical areas in Taiwan. Levofloxacin non-susceptible S. pneumoniae isolates were subsequently analysed for serotype and molecular epidemiology. Rates of levofloxacin non-susceptibility of S. pneumoniae increased significantly from 1.2% in 2001 to 4.2% in 2007 at NTUH. A total of ...
Source: Clinical Microbiology and Infection - September 22, 2009 Category: Microbiology Authors: Y-C. Hsieh, L-Y. Chang, Y-C. Huang, H-C. Lin, L-M. Huang, P-R. Hsueh Source Type: journals
