Clinical and Laboratory HaematologyThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

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Mean cell volume of neutrophils and monocytes compared with C-reactive protein, interleukin-6 and white blood cell count for prediction of sepsis and nonsystemic bacterial infectionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinicians are in need of better diagnostic markers for rapid diagnosis of severe infections. Therefore, we studied the diagnostic significance of mean cell volume of neutrophils (MNV) and monocytes (MMV) compared with Interleukin-6 (IL-6), C-reactive protein (CRP) and white blood cell count for predicting systemic clinical infection (sepsis). MNV and MMV were obtained by volume conductivity scatter (VCS) technique of the Coulter LH 750 hematology analyzer during automated differential counts, and blood samples from patients with sepsis (n = 37), nonsystemic bacterial infections (n = 39) and controls (n = 48) were studied....
Source: Clinical and Laboratory Haematology - November 17, 2009 Category: Hematology Authors: D. MARDI, B. FWITY, R. LOBMANN, A. AMBROSCH Source Type: journals

Application of an expanded multiplex genotyping assay for the simultaneous detection of Hemoglobin Constant Spring and common deletional α-thalassemia mutationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hemoglobin Constant Spring (HbCS) is the most common nondeletional [alpha]-thalassemia variant causing HbH disease, making its detection crucial in populations at risk. Universal newborn screening for HbH is carried out in California. Identification of [alpha]-thalassemia genotypes responsible for HbH and HbH-CS requires rapid, accurate and cost-effective genotyping methods suitable for population screening. We incorporated the HbCS mutation into our existing seven-plex genotyping assay for common [alpha]-thalassemia deletions. To assess the feasibility and diagnostic utility of this expanded multiplex gap-PCR assay, we de...
Source: Clinical and Laboratory Haematology - November 16, 2009 Category: Hematology Authors: J. L. KIDD, M. AZIMI, B. LUBIN, E. VICHINSKY, C. HOPPE Source Type: journals

The utility of the Sysmex XE-2100 analyzer's NEUT-X and NEUT-Y parameters for detecting neutrophil dysplasia in myelodysplastic syndromesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The diagnosis of myelodysplastic syndromes (MDS) is based on morphological changes in the blood and bone marrow. The parameters NEUT-X and NEUT-Y of the Sysmex XE-2100 analyzer could help detect neutrophil dysplasia. A control group of 50 patients, along with 50 postpartum patients, 50 anemias, 50 leukopenias, 50 patients with microscopically visible hypergranulated neutrophils and 50 MDS patients were assessed. The NEUT-X and NEUT-Y values (mean ± SD) for the control group were 1346 ± 28.2 and 420 ± 19.3, respectively, with the anemia and leukopenia groups giving similar values. The postpartum and hypergranulated neutr...
Source: Clinical and Laboratory Haematology - November 10, 2009 Category: Hematology Authors: J. R. FURUNDARENA, M. ARAIZ, M. URANGA, M. R. SAINZ, A. AGIRRE, M. TRASSORRAS, N. URESANDI, M. C. MONTES, N. ARGOITIA Source Type: journals

Audit on the use of coagulation screen in the acute admission unit in a tertiary centreEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - November 2, 2009 Category: Hematology Authors: A. Wakil, J. M. Ng, R. Medlock, E. Masson Source Type: journals

Plasma thrombopoietin level and platelet indices in hemodialysis patients receiving recombinant human erythropoietinEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study included 25 HD patients undergoing treatment with rHuEPO at 9000 IU/week. These patients were divided into two groups by reference PLT of 130 × 109/l [eight patients with low PLT (L-PLT group) and 17 patients with normal PLT (N-PLT group)], and TPO level and platelet indices in each group were compared with those in nine HD patients not receiving rHuEPO. In HD patients, the mean value of MPV was slightly higher and the mean values of PLT, PCT, and MPC were significantly lower than those in healthy controls. TPO levels were significantly higher in patients receiving rHuEPO than in patients not receiving rHuEPO. ...
Source: Clinical and Laboratory Haematology - October 28, 2009 Category: Hematology Authors: M. ASANUMA, K. SEINO, T. MIZUNO, M. NASU, F. YAMAUCHI, M. FUJISHIMA Source Type: journals

Direct international normalized ratio determination using multicalibrators is more responsive than the conventional method for measuring prothrombin timeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Direct international normalized ratio (INR) determination using certified INR plasmas was shown to improve precision and accuracy. We evaluated the utility of a multicalibrator in determining INR. INR values were measured in 493 blood samples from patients subjected to anticoagulation therapy (320) and control subjects (173). Study was performed using CA-7000 coagulation analyzer (Sysmex, Japan) with Thromborel S (Dade Behring, Germany). Direct INR values were obtained using PT-Multi Calibrator (Dade Behring) composed of five lyophilized calibrant plasmas. Conventional INR values were calculated from mean normal prothrombi...
Source: Clinical and Laboratory Haematology - October 28, 2009 Category: Hematology Authors: C.-H. CHA, C.-J. PARK, D. H. KIM, M.-J. KIM, Y.-U. CHO, S. JANG, H.-S. CHI Source Type: journals

Could rapid particle gel immunoassay (ID-PaGIA) replace standard ELISA for laboratory detection of heparin/pf4 antibodies?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - October 28, 2009 Category: Hematology Authors: J. P. Antovic, E.-M. Norberg, M. Sten-Linder Source Type: journals

Hirudin-induced pseudothrombocytopenia in a patient with EDTA-dependent platelet aggregation: report of a new laboratory artefactEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - October 28, 2009 Category: Hematology Authors: C. Robier, M. Neubauer, H. Sternad, F. Rainer Source Type: journals

Expression patterns of specific promyelocytic/retinoic acid receptor-α transcripts in patients with acute promyelocytic leukemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Several additional promyelocytic/retinoic acid receptor-[alpha] (PML/RAR[alpha]) transcripts besides bcr1, bcr2, and bcr3 have been identified in patients with acute promyelocytic leukemia (APL). However, the expression levels of these specific isoforms and their clinical relevance have not been studied to date. The real-time quantitative polymerase chain reaction was established to detect each specific isoform of PML/RAR[alpha] transcripts (bcr1/2, P46R3, P4R3, bcr3, and P2R3) in 46 APL patients. Whereas P46R3 and P4R3 isoforms were concurrently expressed in both bcr1- and bcr2-positive patients, P2R3 isoform was expresse...
Source: Clinical and Laboratory Haematology - October 26, 2009 Category: Hematology Authors: J. LIN, L.-X. HAN, J. QIAN, Y.-L. WANG, D.-M. YAO, Z. QIAN, X.-F. YANG, X.-J. SHENG Source Type: journals

International Council for Standardization in Haematology technical report 1-2009: new reference material for haemiglobincyanide for use in standardization of blood haemoglobin measurementsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The International Council for Standardization in Haematology (ICSH) in conjunction with Eurotrol, B.V. has released a new lot of the haemiglobincyanide or haemoglobin standard. This technical report describes the purpose, methodology in manufacturing, summary of value assignment data and availability of this standard material used for the standardization and calibration of whole blood haemoglobin measurements on most haemoglobinometers and automated blood cell counters throughout the world. (Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - October 26, 2009 Category: Hematology Authors: BRUCE H. DAVIS, BART JUNGERIUS Source Type: journals

Utility of hematologic and volume, conductivity, and scatter parameters from umbilical cord blood in predicting chorioamnionitisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We evaluated the utility of routine hematologic parameters and volume, conductivity, and scatter (VCS) parameters from umbilical cord blood in predicting pathologically confirmed chorioamnionitis. Chorioamnionitis is strongly associated with early neonatal sepsis and is the major in utero source of infectious exposure. We prospectively identified mothers who had placenta submitted for pathology and subsequently identified corresponding neonate cord blood sent for routine blood typing (n = 99). Cord blood was then sent for routine complete blood count with differential (CBCD). Among the same neonates, we retrospectively ide...
Source: Clinical and Laboratory Haematology - September 27, 2009 Category: Hematology Authors: J. C. LEE, T. P. AHERN, F. P. CHAVES, K. QUILLEN Source Type: journals

DNA Index in childhood acute lymphoblastic leukaemia: a karyotypic method to validate the flow cytometric measurementEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The DNA index (DI) is a prognostic factor in childhood acute lymphoblastic leukemia (ALL). The accuracy of DI measurement is important for treatment stratification: hyperdiploidy with DI [ge] 1.16 is predictive of favorable prognosis whereas hypodiploidy is associated with poor prognosis. The aim of this study was to validate the accuracy of the DI measured by flow cytometry (FCM) by comparison with the karyotype. From samples of 112 childhood ALL, we created a formula to calculate a theoretical DNA index (tDI) based on the blast cell karyotype, taking into account the additional or missing chromosome material of the major...
Source: Clinical and Laboratory Haematology - September 27, 2009 Category: Hematology Authors: P. RACHIERU-SOURISSEAU, L. BARANGER, N. DASTUGUE, A. ROBERT, F. GENEVIÈVE, E. KUHLEIN, A. CHASSEVENT Source Type: journals

An evaluation of the SPIFE® 3000 semi-automated gel electrophoresis system for the identification of hemoglobin variants and comparison of relative electrophoretic mobilities with manual gel electrophoresis methodsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Laboratory identification of hemoglobin (Hb) variants can involve multiple techniques. The use of semi-automated instruments that perform gel electrophoresis and staining, such as the SPIFE® 3000 electrophoresis system, can greatly reduce the labor required for these commonly used techniques. We performed a comparison of the method involved in SPIFE® 3000 system with those of manual gel electrophoresis. A total of 22 540 samples were analyzed using the SPIFE® 3000, and compared with mobilities on cellulose acetate and citrate agar gels using standard manual methods. The results were compared using relative electrophoret...
Source: Clinical and Laboratory Haematology - September 16, 2009 Category: Hematology Authors: J. D. HOYER, K. M. MARKLEY, M. E. SAVEDRA, K. S. KUBIK, R. M. SCHEIDT Source Type: journals

Sideroblastic changes of the bone marrow can be predicted by the erythrogram of peripheral bloodEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we analyzed the value of a specific erythrogram pattern from peripheral blood, produced by the ADVIA®120 cell counter, to predict sideroblastic changes in the bone marrow. In a two step-design study, we first showed that 32/38 consecutive patients reporting [ge]15% RS had such a pattern in the erythrogram. In the second step, we prospectively identified over a period of 32 months 21 patients with this typical erythrogram; 20/21 had [ge]15% RS in the bone marrow. Hence, by this validation, we confirm that the erythrogram is highly predictive of RS in the bone marrow. The interpretation of the erythrogram sho...
Source: Clinical and Laboratory Haematology - August 25, 2009 Category: Hematology Authors: A. ROVÓ, G. STÜSSI, S. MEYER-MONARD, G. FAVRE, D. TSAKIRIS, D. HEIM, J. HALTER, C. ARBER, J. PASSWEG, A. GRATWOHL, A. TICHELLI Source Type: journals

Relationships of serum free thyroxine and erythrocyte measures in euthyroid HFE C282Y homozygotes and control subjects: the HEIRS StudyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hemoglobin (Hb) levels and mean corpuscular volume (MCV) are abnormal in some persons with hemochromatosis or thyroid disorders. We sought to determine whether serum free thyroxine (T4) affects erythrocyte measures in euthyroid adults with or without C282Y homozygosity. We evaluated 488 white HFE C282Y homozygotes and controls (no HFE C282Y or H63D; normal serum iron measures) identified in screening; we excluded those with thyroid disorders, anemia, erythrocytosis, or serum ferritin (SF) (Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - August 24, 2009 Category: Hematology Authors: J. C. BARTON, C. LEIENDECKER-FOSTER, D. M. REBOUSSIN, P. C. ADAMS, R. T. ACTON, J. H. ECKFELDT Source Type: journals

Detection of circulating antierythropoietin antibodies in patients with end stage renal disease on regular hemodialysisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recombinant human erythropoietin (rHuEPO) has been successfully and safely used to treat anemia in patients with end stage renal disease (ESRD). The safety profile of rHuEPO had been considered to be excellent with possible exception of hypertension and increased risk of dialysis access thrombosis. Recently, antibody-mediated pure red cell aplasia associated with administration of rHuEPO has been identified as a cause of major concern; we aimed to detect and evaluate the presence of anti-EPO antibodies in patients with ESRD on regular dialysis who are using rHuEPO. Serum anti-EPO antibodies were detected by enzyme-linked i...
Source: Clinical and Laboratory Haematology - August 23, 2009 Category: Hematology Authors: M. M. EL-DIN, F. M. ATTIA, S. M. LABIB, W. OMAR Source Type: journals

Immature platelet fraction measurement in patients with chronic liver disease: a convenient marker for evaluating cirrhotic changeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined whether the measurement of the immature platelet fraction (IPF) in thrombocytopenic patients with liver dysfunction is useful as a rapid and noninvasive method for the differential diagnosis of chronic liver diseases. We examined 20 liver cirrhosis patients, 56 patients with chronic hepatitis, 9 patients with fatty liver, and 86 patients without liver disease. The percentage value of IPF (IPF%) was measured using an XE-2100 multiparameter automatic hematology analyzer. Using a receiver operating characteristic curve, we found diagnostic significance of the absolute platelet count and the absolute number of the ...
Source: Clinical and Laboratory Haematology - August 19, 2009 Category: Hematology Authors: T. NOMURA, Y. KUBOTA, A. KITANAKA, K. KUROKOUCHI, T. INAGE, K. SAIGO, K. ISEKI, N. BABA, G. YAMAOKA, T. ARAI, T. TAMINATO Source Type: journals

Letter to the EditorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - August 18, 2009 Category: Hematology Authors: G. GIBBS Source Type: journals

Cotransplantation of HLA-identical mesenchymal stem cells and hematopoietic stem cells in Chinese patients with hematologic diseasesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mesenchymal stem cells (MSCs) may be employed to support hematopoietic reconstitution and mitigate graft-vs.-host disease (GVHD) in transplantation of hematopoietic stem cells (HSCs). The aim of this study was to explore the feasibility and safety of cotransplantation culture-expanded MSCs and HSCs from the same human leukocyte antigen (HLA)-identical sibling donor in Chinese patients with hematologic diseases. Bone marrow mononuclear cells from healthy donors were cultured and expanded ex vivo. Immunophenotype, adipogenic and osteogenic differentiation potential, and karyotype of the harvested MSCs were detected on those ...
Source: Clinical and Laboratory Haematology - July 27, 2009 Category: Hematology Authors: X. ZHANG, J.-Y LI, K. CAO, H. LU, M. HONG, S. QIAN, H. WU, P. LIU, W. XU Source Type: journals

Establishment of a reference interval for natural killer cell activity through flow cytometry and its clinical application in the diagnosis of hemophagocytic lymphohistiocytosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recently, the Histiocyte Society revised the diagnostic criteria for hemophagocytic lymphohistiocytosis (HLH) to include low or absent natural killer (NK) cell activity, according to local laboratory reference. The aim of this study was to establish reference interval for functional NK-cell activity in 63 healthy Korean individuals using a flow-cytometric assay. We used peripheral blood mononuclear cells (PBMCs) as effector cells and Fluorescein isothiocyanate-labeled K562 cells as target cells. NK-cell activity was calculated using the following equation: NK-cell activity (%) = (test lysis [minus] spontaneous lysis) × 10...
Source: Clinical and Laboratory Haematology - July 14, 2009 Category: Hematology Authors: H. J. CHUNG, C. J. PARK, J. H. LIM, S. JANG, H. S. CHI, H. J. IM, J. J. SEO Source Type: journals

Telomerase activity and telomere length in acute leukemia: correlations with disease progression, subtypes and overall survivalEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The progressive shortening of telomeres and the activation of telomerase are considered to be one of the important mechanisms in cellular immortalization and disease progression. Bone marrow samples were collected from 148 patients with acute leukemia (AL). Based on the stage of the disease, patients were divided into the newly diagnosed group, the relapsed group and the complete remission (CR) group. telomerase activity (TA) was examined by PCR-ELISA, and telomere length (TL) was examined by Southern blot analyses. TA and TL were analyzed in relation to AL stage and subtype. Five-year survival was analyzed using Kaplan[nd...
Source: Clinical and Laboratory Haematology - July 14, 2009 Category: Hematology Authors: Y. WANG, M. FANG, X. SUN, J. SUN Source Type: journals

An automated anti-IIa assay to measure UFH levels in plasma – a method without exogenous antithrombinEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - July 13, 2009 Category: Hematology Authors: R. G. Summerhayes, F. Newall, P. Monagle, V. Ignjatovic Source Type: journals

Usefulness of measuring reticulocyte hemoglobin equivalent in the management of haemodialysis patients with iron deficiencyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, to clarify the accuracy of RET-He in diagnosing iron deficiency in dialysis patients, we initially compared RET-He with such iron parameters as serum ferritin levels, transferrin saturation and content of reticulocyte hemoglobin (CHr) which has been established as indicators of functional iron deficiency. Secondly, we investigated the changes in RET-He during iron supplementation for iron-deficient patients to determine whether this marker is a prospective and reliable indicator of iron sufficiency. The participants in this study were 217 haemodialysis patients. Iron deficiency was defined as havsing a trans...
Source: Clinical and Laboratory Haematology - July 13, 2009 Category: Hematology Authors: N. MIWA, T. AKIBA, N. KIMATA, Y. HAMAGUCHI, Y. ARAKAWA, T. TAMURA, K. NITTA, K. TSUCHIYA Source Type: journals

Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropeniaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Our results suggest that diagnosis of immune Np in the laboratory may be improved by focusing on patient's PMN together with the assessment of cellular markers. (Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - July 5, 2009 Category: Hematology Authors: N. E. RIERA, M. ROSSO SALTÓ, V. GALÁN, K. CANALEJO, M. KHOURY, M. AIXALÁ, G. L. KANTOR, M. VERMEULEN, R. BENGIÓ, M. M. E. DE BRACCO Source Type: journals

Myeloablative hematopoietic stem cell transplantation for myelodysplastic syndrome in patients younger than 55 years: impact of comorbidity and disease burden on the long-term outcomeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We retrospectively investigated 31 myelodysplastic syndrome (MDS) patients receiving myeloablative hematopoietic stem cell transplantation (HCT) and focused on prognostic factors affecting the long-term outcome. Patients were classified according to the French-American-British classification and the HCT-comorbidity index was determined. Cytosine arabinoside or thiotepa combined with cyclophosphamide and total body irradiation was used as myeloablative conditioning in eight and 23 patients respectively. After a follow-up period of 0.8[ndash]14.2 years from transplantation (median: 6.4 years), 23 patients were alive in compl...
Source: Clinical and Laboratory Haematology - July 4, 2009 Category: Hematology Authors: H. KANAMORI, M. ENAKA, S. ITO, K. MOTOHASHI, M. HAGIHARA, R. OSHIMA, R. SAKAI, S. FUJISAWA, M. TANAKA, K. FUJIMAKI, H. FUJITA, Y. ISHIGATSUBO, A. MARUTA Source Type: journals

RBC-Y/MCV as a discriminant function for differentiating carriers of thalassaemia and HbE from iron deficiencyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Individuals with [alpha]-thalassaemia (ATT), [beta]-thalassaemia (BTT) and HbE trait (HET) are often initially identified based on haematological parameters. However, the values of these parameters usually overlap with iron deficiency anaemia (IDA) and anaemia of chronic disease (ACD). We evaluated the use of RBC-Y in 156 normal individuals and 332 patients; ATT (n = 37), BTT (n = 61), HET (n = 25), HbH disease (n = 5), ACD (n = 67), IDA (n = 83) and ACD with IDA (n = 54). Diagnostic efficiency was analysed by receiver operating characteristics (ROC). MCH was better compared with RBC-Y in discriminating normal from abnorma...
Source: Clinical and Laboratory Haematology - June 28, 2009 Category: Hematology Authors: V. S. NADARAJAN, P. STHANESHWAR, S. JAYARANEE Source Type: journals

Myelodysplastic syndromes: the role of flow cytometry in diagnosis and prognosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Flow cytometric immunophenotypic analysis is a well-recognized and widely accepted adjuvant test to increase the sensitivity and specificity of diagnosis in potential myelodysplastic syndrome (MDS) cases. In addition, flow cytometric analysis provides prognostic information in MDS that is not available from other sources. The flow cytometric findings indicative of MDS and its value is a diagnostic adjunct are discussed. (Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - June 25, 2009 Category: Hematology Authors: M. STETLER-STEVENSON, C. M. YUAN Source Type: journals

Evaluating glutathione S-Transferase (GST) null genotypes (GSTT1 and GSTM1) as a potential biomarker of predisposition for developing leukopeniaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Glutathione S-transferase (GST) enzymes protect cells against xenobiotics and oxidative stress products through an electrophilic conjugation process. We investigated the theta (GSTT1) and mu (GSTM1) null genotypes in a group of leukopenic subjects and normal subjects from Northeast Brazil, evaluating their use as biomarkers of susceptibility for developing leukopenia. In a sample-based case[ndash]control study, we analysed white blood cell (WBC) counts and GSTT1 and GSTM1 genotypes. A total of 278 subjects were analysed: 91 with leukopenia and 187 controls. GSTT1 null genotype conferred a 5.92-fold risk for occurrence of l...
Source: Clinical and Laboratory Haematology - June 24, 2009 Category: Hematology Authors: M. S. GONCALVES, J. P. MOURA NETO, C. L. SOUZA, P. MELO, M. G. REIS Source Type: journals

BAALC and ERG expression in acute myeloid leukemia with normal karyotype: impact on prognosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cytogenetic aberrations are important prognostic factors in acute myeloid leukemia (AML). About 45% of de novo AML lack cytogenetic abnormalities, so identification of predictive molecular markers might improve therapy. We studied the prognostic impact of brain and acute leukemia, cytoplasmic (BAALC) and ETS-related gene (ERG) expression in AML with normal karyotype. Pretreatment bone marrow samples from 30 cytogenetically normal AML patients were analysed for BAALC and ERG expression using real time RT-PCR. The patients were dichotomized at BAALC and ERG mean expression into low and high expression. BAALC showed high expr...
Source: Clinical and Laboratory Haematology - June 22, 2009 Category: Hematology Authors: M. A. EID, M. ATTIA, S. ABDOU, S. F. EL-SHAZLY, L. ELAHWAL, W. FARRAG, L. MAHMOUD Source Type: journals

Photochemical decomposition of dipyridamole in aqueous solution and the utilization of citrate–theophylline–adenosine–dipyridamole anticoagulant for monitoring of heparinEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - June 21, 2009 Category: Hematology Authors: A. M. H. P. van den Besselaar Source Type: journals

Quantitation of whole blood Epstein-Barr virus DNA is useful for assessing treatment response in patients with non-Hodgkin's lymphomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion. These results suggest that whole blood EBV-DNA quantitation might be of value as a convenient biomarker for therapeutic responsiveness of NHL. (Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - June 16, 2009 Category: Hematology Authors: S. A. JO, S.-H. HWANG, S. Y. KIM, H.-J. SHIN, J. S. CHUNG, M. Y. SOL, C. L. CHANG, E. Y. LEE Source Type: journals

International Normalized Ratio (INR), coagulation factor activities and calibrated automated thrombin generation – influence of 24 h storage at ambient temperatureEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
International Normalized Ratio (INR) measurements are used to monitor oral anticoagulation therapy with coumarins. Single coagulation factor activities and calibrated automated thrombin (CAT) generation are considered as more advanced methods for evaluating overall haemostatic capacity. The aims were to assess the variability of INR, coagulation factor activities, and CAT, during 24 h of storage of blood samples at ambient temperature. A total of 24 patients on stable coumarin treatment were followed prospectively for 6 weeks. INR was analyzed at 0, 6 and 24 h after blood sampling and 1-stage clotting activity of coagulati...
Source: Clinical and Laboratory Haematology - May 27, 2009 Category: Hematology Authors: T. D. CHRISTENSEN, C. JENSEN, T. B. LARSEN, M. MAEGAARD, K. CHRISTIANSEN, B. SØRENSEN Source Type: journals

Genomic analysis of acute leukemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Acute leukemia is the commonest childhood cancer and a major cause of morbidity from hematologic malignancies in adults. Acute lymphoblastic leukemia (ALL) is commonest in children, and acute myeloid leukemia (AML) is more frequent in adults. Apart from childhood ALL, the prognosis of acute leukemia is suboptimal, with many patients experiencing relapse, which carries a poor prognosis, or toxicities from nonspecific therapies. Recent years have witnessed great interest in the application of high-resolution, genome wide approaches to the study of acute leukemia. These studies have identified multiple novel genetic alteratio...
Source: Clinical and Laboratory Haematology - May 19, 2009 Category: Hematology Authors: C. G. MULLIGHAN Source Type: journals

Real-time PCR quantification of haematopoietic chimerism after transplantation: a comparison between TaqMan and hybridization probes technologiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to compare the sensitivity and accuracy of two methods of quantitative real-time polymerase chain reaction (qrt-PCR), in order to determine haematopoietic chimerism (CH): single nucleotide polymorphisms using TaqMan (TM) probes and insertion/deletion polymorphisms using Hybridization (Hyb) probes. A total of 106 samples from 20 patients who underwent allogenic stem cell transplantation (n = 14) or live-donor liver transplantation (n = 6) were studied. The mean level of chimerism was 8.37% for the TM method and 7.73% in the Hyb method, which was not significantly different (P = 0.69). The Pearson correlatio...
Source: Clinical and Laboratory Haematology - May 15, 2009 Category: Hematology Authors: J. MARTINEZ-LOPEZ, A. CROOKE, S. GRANDE, R. AYALA, A. JIMÉNEZ-VELASCO, S. GAMARRA, J. C. MENEU, F. GILSANZ Source Type: journals

Investigation of the distribution of lymphocyte subsets and zinc levels in multitransfused β-thalassemia major patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to utilize flow cytometric immunophenotyping to characterize effects of regular blood transfusion, and high serum ferritin levels because of irregular use of iron chelation therapy on T lymphocytes (CD2, CD3, CD4 and CD8), B lymphocytes (CD19) and natural killer cells (CD56) and zinc levels in the blood of patients with thalassemia major (n = 49) and healthy normal controls (n = 60) in Kuwait. None of the patients had active infections. T-cell markers' percentage levels were comparable between patients and controls (P > 0.05), while B cell marker (CD19) was significantly higher in patients (P = 0.00...
Source: Clinical and Laboratory Haematology - April 24, 2009 Category: Hematology Authors: A. M. AL-AWADHI, S. M. AlFADHLI, D. AL-KHALDI, M. BORHAMA, M. BORUSLY Source Type: journals

A novel hemoglobin variant beta135(H13) Ala > Asp identified in an asymptomatic Korean family by direct sequencing: suggesting a new insight into Hb Beckman mutationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This article describes the clinical observation of a novel hemoglobin (Hb) variant found during the course of routine blood testing on a 61-year-old subject. The Hb variant was observed during HbA1c testing by ion-exchange high-performance liquid chromatography. Alkaline electrophoresis and DNA sequencing confirmed the presence of a new Hb variant, HBB:c.407C > A (p.Ala136Asp). This mutation has been reported to induce Hb Beckman variant in the Globin Gene Server. However, it was different from the only experimental report for Hb Beckman by Rahbar, Lee & Asmeron (p.Ala136Glu; Hb Beckman alpha2 beta2 135(H13) ala-to-glu: a ...
Source: Clinical and Laboratory Haematology - April 22, 2009 Category: Hematology Authors: S. Y. KIM, G. Y. J. KIM, S. A. JO, E. H. LEE, E. H. CHO, S. H. HWANG, E. Y. LEE Source Type: journals

Multiple myeloma presenting with an acute bacterial infectionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Increased susceptibility to bacterial infections is a common manifestation of multiple myeloma (MM), arising mainly from a defect in humoral immunity and is associated with major morbidity and mortality. The propensity to infection is increased in the first months after the initial diagnosis and in patients with renal dysfunction. Gram-positive infections, mainly pneumonia from Streptococcus pneumoniae, occur more frequently in patients with untreated disease while Gram-negative infections, mainly of the urinary tract, are more common after chemotherapy. However, an acute bacterial infection is rarely reported as the first...
Source: Clinical and Laboratory Haematology - April 20, 2009 Category: Hematology Authors: G. N. KALAMBOKIS, L. CHRISTOU, E. V. TSIANOS Source Type: journals

Evaluating five dedicated automatic devices for haemoglobinopathy diagnostics in multi-ethnic populationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We have tested five haemoglobin (Hb) separation apparatuses, dedicated to haemoglobinopathy diagnostics. These are the four high performance liquid chromatography devices: VARIANT II[trade], HA 8160, G7, Ultra2 and the Capillary Electrophoresis apparatus from Sebia. In the first place, we focussed on the capacity of all apparatuses to detect the most common structural variants relevant for public health, these being HbS, HbC, HbE, HbD-Punjab and HbO-Arab. We then compared how the high HbA2[beta]-thalassaemia carriers were identified. All apparatuses were able to identify carriers of these traits with the expected sensitivi...
Source: Clinical and Laboratory Haematology - April 20, 2009 Category: Hematology Authors: P. VAN DELFT, E. LENTERS, M. BAKKER-VERWEIJ, M. de KORTE, U. BAYLAN, C. L. HARTEVELD, P. C. GIORDANO Source Type: journals

Berend Houwen Memorial Lecture: ISLH Las Vegas May 2009Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Thrombotic microangiopathies are a relatively rare group of congenital and inherited disorders caused by defects in processing the ultra large forms of von Willibrand factor which pathologically give rise to platelet rich microthrombi in the micro arterial circulation leading to end organ damage particularly in the brain, heart and kidneys. Identification of the ADAMTS 13 gene has led to the definition of congenital deficiency of its activity or failure of activity due to the development of an inhibitory IgG antibody. The idiopathic autoimmune form of the disease is the most common. There are various subgroups of acquired ...
Source: Clinical and Laboratory Haematology - April 20, 2009 Category: Hematology Authors: M. A. SCULLY, S. J. MACHIN Source Type: journals

Quality counts: new parameters in blood cell countingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recently several parameters have been introduced to the complete blood count such as nucleated red blood cells, immature granulocytes; immature reticulocyte fraction, immature platelet fraction and red cell fragments as well as new parameters for detection of functional iron deficiency. Leucocyte positional parameters, which may diagnose specific diseases (e.g. differentiate between abnormal lymphocytes in leukaemia and viral conditions and may also detect malarial infection) are now available. At this time they are only used for research; however, generally such parameters later become reportable. One manufacturer's routi...
Source: Clinical and Laboratory Haematology - April 17, 2009 Category: Hematology Authors: C. BRIGGS, I. MELLORS, A. RODERICK, A. WARD, C. O'MALLEY, J. BARKER, B. DE LA SALLE, P. McTAGGART, K. HYDE, S. J. MACHIN Source Type: journals

Clinical and haematological features in a compound heterozygote (HBB:c.92 + 5G > C/HBB:c.93-2A > C) case of thalassaemia majorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This report confirms the presence of HBB:c.93-2A > C in the Indian subcontinent and has important implications for screening and prenatal diagnosis of beta thalassaemia. This report also supports inclusion of this mutation in the beta globin gene mutation database. (Source: Clinical and Laboratory Haematology)
Source: Clinical and Laboratory Haematology - April 13, 2009 Category: Hematology Authors: S. AGARWAL, P. M. TAMHANKAR, R. KUMAR, A. DALAL Source Type: journals

Clinicovirologic analysis of hepatitis C infection in transfusion-dependent β-thalassemia major childrenEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Regular blood transfusion puts [beta]-thalassemia major patients at a higher risk of developing hepatic iron overload and hepatitis C virus (HCV) infection. The association between several transfusion-related factors and an increased risk of developing HCV viremia has been reported. The effect of HCV infection on liver damage in transfusion-dependent thalassemia patients has been poorly described. A sample of 100 Egyptian transfusion-dependent [beta]-thalassemia major children were studied. Individual patients underwent full history taking, clinical examination and a panel of laboratory tests including HCV ribonucleic acid...
Source: Clinical and Laboratory Haematology - April 7, 2009 Category: Hematology Authors: L. RAGAB, S. HELAL, N. ZAGHLOUL, M. EL-RAZIKY, R. AFIFI, K. M. MUSALLAM, A. T. TAHER Source Type: journals

British Society for Haematology, slide session, annual scientific meeting, Glasgow, 2008Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A morphology session is held each year at the Annual Scientific Meeting of the British Society of Haematology. Prior to the meeting this year, eight morphology cases were made available to BSH members as glass slides and also digitally as 'virtual slides'. A panel of invited commentators who had no prior knowledge of the diagnosis discussed the eight cases. An initial limited history and blood count are given with representative images from the case material; this is followed by the discussants' comments and suggested diagnosis. The actual clinical diagnosis is then given with other relevant information. (Source: Clinical ...
Source: Clinical and Laboratory Haematology - April 2, 2009 Category: Hematology Authors: C. V. HUTCHINSON, J. BURTHEM, M. BISLAND, P. CAREY, G. CROTTY, V. DEVALIA, B. JANDA, W. GORDON, C. N. HARRISON, J. MURRAY, A. SHLEBAK, A. THOMAS, B. WILKINS, M. F. McMULLIN Source Type: journals

Can Thromboelastography performed on kaolin-activated citrated samples from critically ill patients provide stable and consistent parameters?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Thromboelastography (TEG®) is a potentially useful tool but analysis within 4-6 min of collection imposes limitations on its use and access. The use of citrate blood tubes potentially increases the time frame for processing specimens. There is, however, limited research on the stability of citrate specimens, timing of processing and the accuracy of TEG® results. The purpose of this study was to examine the effects of early and delayed processing on TEG® parameters using kaolin-activated citrated blood samples in the intensive care population. TEG® analysis was performed on 61 patients. Blood was collected into two 3.2%...
Source: Clinical and Laboratory Haematology - March 20, 2009 Category: Hematology Authors: H. WHITE, C. ZOLLINGER, M. JONES, R. BIRD Source Type: journals

Expression profile of Notch-related genes in multidrug resistant K562/A02 cells compared with parental K562 cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Drug resistance is the major setback of acute myeloid leukemia (AML) therapy. Notch proteins have demonstrated functional regulation in cell proliferation, differentiation, and apoptosis and thus may affect drug resistance. Our study aimed to identify the Notch-related gene profile in drug-resistant AML cells and provide potential strategies for resistant AML therapy. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted to detect the cytotoxicity of adriamycin toward K562 and drug-resistant K562/A02. Intracellular mean fluorescence intensity was monitored to reflect the intake of adriamycin by c...
Source: Clinical and Laboratory Haematology - March 17, 2009 Category: Hematology Authors: S. YAN, D. MA, M. JI, D. GUO, J. DAI, P. ZHAO, C. JI Source Type: journals

Apoptosis inducing and differentiation enhancement effect of oridonin on the all-trans-retinoic acid-sensitive and -resistant acute promyelocytic leukemia cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We investigated the effects of oridonin (Ori), a diterpenoid isolated from Rabdosia rubescens, on apoptosis and differentiation of all-trans-retinoic acid (ATRA)-sensitive (NB4) and ATRA-resistant (NB4-R1) cells. The results showed that reactive oxygen species initiates Ori-induced apoptosis. In addition, we found that neither Ori nor ATRA (10 nm) alone induced marked cell differentiation, while co-treatment of these two compounds can induce differentiation of NB4 and NB4-R1 cells which was accompanied by increased RAR[alpha], C/EBP[epsilon] or C/EBP[beta]. This is the first report to show that RAR[alpha] could be accumula...
Source: Clinical and Laboratory Haematology - March 14, 2009 Category: Hematology Authors: F. GAO, Q. TANG, P. YANG, Y. FANG, W. LI, Y. WU Source Type: journals

Fresh-frozen, optimal cutting temperature (OCT) compound-embedded bone marrow aspirates: a reliable resource for morphological, immunohistochemical and molecular examinationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The usefulness of fresh-frozen, optimal cutting temperature (OCT) compound-embedded (FFOE) bone marrow (BM) aspirates was evaluated as a reliable resource for morphological, immunohistochemical and molecular examinations. One hundred BM aspirates were collected in polypropylene tubes and immediately frozen for 2 h in a deep freezer. Frozen BM was transferred to a cryomold filled with OCT compound and the prepared samples were stored in a deep freezer. Histological examination and immunohistochemical staining, polymerase chain reaction (PCR), sequencing and reverse transcription (RT)-PCR were performed to evaluate the quali...
Source: Clinical and Laboratory Haematology - March 9, 2009 Category: Hematology Authors: J. LIM, Y. KIM, W. LEE, M. KIM, E. J. LEE, C. S. KANG, K. HAN Source Type: journals

Enhanced expression of CD71, transferrin receptor, on immature reticulocytes in patients with paroxysmal nocturnal hemoglobinuriaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Erythroid cells in the marrow express CD71, transferrin receptor, and reticulocytes released from the marrow lose their expression during maturation. The immature reticulocyte fraction, the proportion of reticulocytes with the highest content of RNA, has been determined by hematology analysis. In the present study, we examined CD71 expression on immature reticulocytes by flow cytometry (FCM) in paroxysmal nocturnal hemoglobinuria (PNH) patients with reticulocytosis. We modified 'reticulocyte-gated FCM' to multi-color FCM, i.e. RNA/CD71, RNA-CD59 or CD59/CD71/CD45. In PNH, in addition to the increased number of immature ret...
Source: Clinical and Laboratory Haematology - March 6, 2009 Category: Hematology Authors: S. SATO, Y. KOZUMA, Y. HASEGAWA, H. KOJIMA, S. CHIBA, H. NINOMIYA Source Type: journals

Detecting methylation patterns of p16, MGMT, DAPK and E-cadherin genes in multiple myeloma patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the methylation status of four genes; p16, O6-methyl guanine DNA methyl transferase (MGMT), death-associated protein kinase (DAPK) and E-cadherin (ECAD); at the time of diagnosis was investigated using methylation-specific polymerase chain reaction (MS-PCR). In the 20 cases studied; methylation of the promoter regions of p16, MGMT, DAPK and ECAD genes was detected in 10%, 40%, 10% and 45% of the cases, respectively. In 65% (13/20) of cases, at least one of the genes studied had promoter methylation; while 35% of cases (7/20) had methylated promoters of more than one gene. There was a significant correlation ...
Source: Clinical and Laboratory Haematology - March 4, 2009 Category: Hematology Authors: O. OZALP YUREGIR, E. YURTCU, E. KIZILKILIC, N. E. KOCER, H. OZDOGU, F. I. SAHIN Source Type: journals

Clinical and hematological study for Parvovirus b19 infection in children with acute leukemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to detect parvovirus B19 DNA together with its antibodies in the sera of children with recent acute leukemia and those with acute leukemia receiving chemotherapy to clarify the contribution of this infection to changes observed in hematological and clinical presentations in these populations. Two groups were included: Group I comprised 45 children with acute leukemia receiving chemotherapy and Group II comprised 40 children with recently diagnosed acute leukemia. Serum parvovirus B19 IgG and IgM were investigated by enzyme-linked immunosorbant assay and the virus DNA was sought by polymerase chain reaction...
Source: Clinical and Laboratory Haematology - February 28, 2009 Category: Hematology Authors: M. E. S. ZAKI, R. E. ASHRAY Source Type: journals