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260 records returned

Characterization of cold-adapted Atlantic cod (Gadus morhua) trypsin I -kinetic parameters, autolysis and thermal stability.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed at further characterization of this enzyme with respect to kinetic parameters, sites of autolysis and stability. For that purpose, trypsin I was purified by anion exchange chromatography. Its purity and identity was verified by SDS-PAGE analysis and mass spectrometry. Concomitantly, another cod trypsin isozyme, trypsin X, previously only described from its cDNA sequence was detected in a separate peak from the ion exchange chromatogram. There was a stepwise increase in the catalytic efficiency (k(cat)/K(m)) of cod trypsin I obtained with substrates containing one to three amino acid residues. As expected, ...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 11, 2009 Category: Biochemistry Authors: Stefansson B, Helgadóttir L, Olafsdottir S, Gudmundsdóttir A, Bjarnason JB Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Partial purification and characterization of Helicoverpa armigera (Lepidoptera: Noctuidae) active aminopeptidase secreted in midgut.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report the partial purification to apparent homogeneity of a soluble aminopeptidase (EC 3.4.11.1) from midgut of Helicoverpa armigera larvae, which preferentially degraded Leucine p-nitroanilide (LpNA). After midgut isolation, extraction and precipitation of soluble proteins with acetone, proteins were purified in two consecutive steps including gel filtration and ion exchange chromatographies. Aminopeptidase activity was increased 8.95 fold after gel filtration chromatography. The purified enzyme appeared as single band with molecular mass of approximately 112kDa in SDS-PAGE, with a pH optimum of 7.0. Zymogram analysis...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 10, 2009 Category: Biochemistry Authors: Lomate PR, Hivrale VK Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Cloning and functional characterisation of a fatty acyl elongase from southern bluefin tuna (Thunnus maccoyii).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The synthesis of long chain polyunsaturated fatty acids (LCPUFA), such as eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3), involves fatty acyl desaturase and elongase enzymes. The marine fish species southern bluefin tuna can accumulate a large quantity of omega-3 (n-3) LCPUFA in their flesh but their capacity to synthesise EPA and DHA is uncertain. A cDNA, sbtElovl5, encoding a putative fatty acyl elongase was amplified from SBT liver tissue. The cDNA included an open reading frame (ORF) encoding 294 amino acids. Sequence comparisons and phylogenetic analyses revealed a high level of seque...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 8, 2009 Category: Biochemistry Authors: Gregory MK, See VH, Gibson RA, Schuller KA Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

A lactose specific lectin from the sponge Cinachyrella apion: Purification, characterization, N-terminal sequences alignment and agglutinating activity on Leishmania promastigotes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Crude extract from the sponge Cinachyrella apion showed cross-reactivity with the polyclonal antibody IgG anti-CvL (Cliona varians lectin) and also a strong haemmaglutinating activity towards human erythrocytes of all ABO groups. Thus, it was submitted to acetone fractionation, IgG anti-deglicosilated CvL Sepharose affinity chromatography, and Fast Protein Liquid Chromatography (FPLC-AKTA Purifier) gel filtration on a Superose 6 10/300 column to purify a novel lectin. C. apion lectin (CaL) agglutinated all types of human erythrocytes with preference for papainized type A erythrocytes. The haemagglutinating activity is ...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 7, 2009 Category: Biochemistry Authors: Medeiros DS, Medeiros TL, Ribeiro JK, Monteiro NK, Migliolo L, Uchoa AF, Vasconcelos IM, Oliveira AS, de Sales MP, Santos EA Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Respiratory proteins in Sipunculus nudus - Implications for phylogeny and evolution of the hemerythrin family.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Three major classes of respiratory proteins are known, hemoglobin, molluscan and arthropod hemocyanin, and hemerythrin (Hr). Similar to hemoglobin, respiratory Hr is packed into erythrocytes floating in the coelomic fluid and is only known from sipunculids, brachiopods, and priapulids. Owing to this scattered distribution, the presence of Hr is generally assumed to be the plesiomorphic condition without phylogenetic importance. By sequencing 2,000 Expressed Sequence Tags (ESTs) from Sipunculus nudus, we found 75 Hr-coding ESTs assembled to 20 cDNA contigs classified as four distinct Hr isoforms: three polymeric Hrs (su...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 5, 2009 Category: Biochemistry Authors: Meyer A, Lieb B Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Molecular cloning and functional analysis of the zebrafish follicle-stimulating hormone (FSH)beta promoter.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study, we cloned and characterized a zebrafish follicle-stimulating hormone (zfFSH)beta promoter with deletion fragments transfected into a tilapia ovary (TO2) cell line, and demonstrated that the zfFSHbeta promoter responded to 6h of gonadotropin-releasing hormone (GnRH) treatment by activating calcium influx and protein kinase C (PKC), but after 24h, GnRH induction was generated by activation of extracellular-regulated kinase (ERK)1/2 and repression by PKC. Furthermore, to study the promoter-specific expression, we constructed a series of FSHbeta (4.0-, 3.0-, 2.0-, and 1.0-kb) promoter-driven green flu...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 4, 2009 Category: Biochemistry Authors: Chen JY, Chiou MJ, Chen LK, Wu JL Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Prospecting for cellulolytic activity in insect digestive fluids.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe quantitative determinations of the cellulolytic activity in gut or head-derived fluids from 68 phytophagous or xylophagous insect species belonging to eight different taxonomic orders. Enzymatic activity was determined for two different substrates, carboxymethyl cellulose (CMC) and microcrystalline cellulose (MCC), approximating endo-beta-1,4-glucanase and complete cellulolytic activity, respectively. Highest CMC gut fluid activities were found in Dictyoptera, Coleoptera, Isoptera, and Orthoptera, while highest MCC gut fluid activities were found in Coleoptera, Hymenoptera, Lepidoptera, and Orthoptera. In most ...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 3, 2009 Category: Biochemistry Authors: Oppert C, Klingeman WE, Willis JD, Oppert B, Jurat-Fuentes JL Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Regulation, expression and characterization of aromatase (cyp19b1) transcripts in ovary and testis of rainbow trout (Oncorhynchus mykiss).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cytochrome P450 aromatase is the key enzyme in the pathway that converts androgens to estrogens. The enzyme functions in the smooth endoplasmic reticulum in a complex with NADPH-cytochrome P450 reductase. In teleost fish, at least two separate loci, cyp19a and cyp19b, encode distinct aromatase isoforms. The activity of cyp19a and cyp19b are predominantly associated with the ovary and brain, respectively, although their expression is not confined solely to these tissues. We found that at least five cyp19b1 transcripts with different 5'-UTRs are generated in the ovary and testis of rainbow trout. Regulation for selection...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 3, 2009 Category: Biochemistry Authors: von Schalburg KR, Yasuike M, Davidson WS, Koop BF Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Comparison of fatty acid composition in nine organs of the sympatric Antarctic teleost fish species Notothenia coriiceps and Notothenia rossii (Perciformes: Nototheniidae).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Fatty acid (FA) composition of nine organs from two closely related Antarctic fish species, Notothenia coriiceps and Notothenia rossii, was determined through gas chromatography with flame ionization detection. A data set for each species was obtained using major FA profiles from specimens caught in the sea waters of Admiralty Bay during the summer season. The FA profile for both species are overall similar, but organ peculiarities have been found, which could reflect metabolic specificities and feeding habits between species. With the exception of liver, the most abundant FA in organs were the n-3 polyunsaturated FA. ...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 30, 2009 Category: Biochemistry Authors: Magalhães BS, Fiamoncini J, Deschamps FC, Curi R, Silva LP Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Isolation and characterization of two types of beta-1,3-glucanases from the common sea hare Aplysia kurodai.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Two types of beta-1,3-glucanases, AkLam36 and AkLam33 with the molecular masses of 36kDa and 33kDa, respectively, were isolated from the digestive fluid of the common sea hare Aplysia kurodai. AkLam36 was regarded as an endolytic enzyme (EC 3.2.1.6) degrading laminarin and laminarioligosaccharides to laminaritriose, laminaribiose, and glucose, while AkLam33 was regarded as an exolytic enzyme (EC 3.2.1.58) directly producing glucose from polymer laminarin. AkLam36 showed higher activity toward beta-1,3-glucans with a few beta-1,6-linked glucose branches such as Laminaria digitata laminarin (LLam) than highly branched be...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 30, 2009 Category: Biochemistry Authors: Kumagai Y, Ojima T Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Structure and stability of crustacean lipovitellin: Influence of lipid content and composition.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Lipovitellin (LV) is essential in crustacean eggs for embryo viability and development. Two LV were isolated from eggs of Macrobrachium borellii. corresponding to early (LVe ) and late (LVl) embryo developing stages. They differ in lipid composition but not in lipid/protein ratio or apoprotein composition. Structural information was obtained by fluorescence spectroscopy, far-UV circular dichroism, partial trypsinolysis and electron microscopy applied to LVe and LVl and two partially delipidated forms of LVe generated by phospholipase A2 (LVp) or triton X-100 (LVt) treatment. All LV forms contained two apoprotein subuni...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 28, 2009 Category: Biochemistry Authors: Garcia CF, Cunningham M, Soulages JL, Heras H, Garda HA Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

CBP Turns Fifty.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19878735 [PubMed - as supplied by publisher] (Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology.)
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 27, 2009 Category: Biochemistry Authors: Mommsen TP, Nilsson GE, Walsh PJ Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

A bombesin-like peptide from skin of Sanguirana varians.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bombesin-like peptides (BLPs) are a family of neuro-endocrinic peptides that mediates a variety of biological activities. A BLP named bombesin-SV from the skin secretions of the frog, Sanguirana varians, was purified and characterized. Its amino acid sequence is pEMIFGAPMWALGHLM-NH2 determined by Edman degradation and mass spectrometry. The cDNA encoding bombesin-SV precursor was cloned from skin cDNA library of S. varians. The precursor is composed of 67 amino acid residues including a copy of mature bombesin-SV. Two predicted enzymatic processing sites (-Lys-Arg-) are flanked at the mature bombesin-SV sequence. In th...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 23, 2009 Category: Biochemistry Authors: Miao Y, Li W, Duan L, Xiao Y Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we report an IRF-2 gene isolcated from orange-spotted grouper (Epinephelus coioides). The 1854bp full length cDNA sequence of EcIRF-2 has been cloned, encoding a putative peptide of 336 amino acids which is highly consistent with the feature of IRF family members. The genomic fragment of EcIRF-2 contains nine exons and eight introns, spanning over approximate 8.8 kb. The expression of EcIRF-2 gene was detected in various tissues of healthy orange-spotted grouper and in four tissues after being challenged with poly I:C or LPS. EcIRF-2 gene is ubiquitously expressed in various healthy fish tissues and is upreg...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 23, 2009 Category: Biochemistry Authors: Shi Y, Zhao Z, Yin JK, Zhu XP, Chen KC, Pan DB, Gui JF Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Isolation and biochemical characterisation of lipid rafts from Atlantic cod (Gadus morhua) intestinal enterocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Lipid rafts are glycosphingolipid/cholesterol-enriched membrane microdomains that have been extensively studied during the past two decades. Our aim was to isolate and perform biochemical characterization of lipid rafts from the intestinal brush border membrane (BBM) of Atlantic cod (Gadus morhua) to confirm their existence in a cold-water species and compare their characteristics with lipid rafts from other species in terms of lipid and protein content. To validate the isolation process, we assayed marker enzymes for subcellular organelles, including alkaline phosphatase (AP) and leucine aminopeptidase (LAP), both wel...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 22, 2009 Category: Biochemistry Authors: Gylfason GA, Knútsdóttir E, Asgeirsson B Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Molecular characterization of trypsinogens and development of trypsinogen gene expression and tryptic activities in grass carp (Ctenopharyngodon idellus) and topmouth culter (Culter alburnus).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study examined the gene structures and expression of trypsinogens, as well as the trypsin activities of the grass carp Ctenopharyngodon idellus (herbivorous) and the topmouth culter Culter alburnus (carnivorous), which are commercially important freshwater species of the family Cyprinidae in China. Isolated full-length trypsinogen cDNA clones were 869bp and 857bp. The deduced amino acid sequences were 242aa and 247aa long, both containing the highly conserved residues essential for serine protease catalytic and conformational maintenance. The results from isoelectric and phylogenetic analyses suggest that grass carp t...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 22, 2009 Category: Biochemistry Authors: Ruan GL, Li Y, Gao ZX, Wang HL, Wang WM Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Five novel antimicrobial peptides from skin secretions of the frog, Amolops loloensis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
While investigating antimicrobial peptide diversity of Amolops loloensis, five novel antimicrobial peptides belonging to two families were identified from skin secretions of this frog. The first family including two members is esculentin-2-AL (esculentin-2-ALa and -ALb); the second family including three members is temporin-AL (temporin-ALd to -ALf). The family of esculentin-2-AL is composed of 37 amino acid residues (aa); the family of temporin-AL is composed of 16, 13 and 10 aa, respectively. All of these antimicrobial peptides showed antimicrobial activities against tested microorganisms. cDNAs encoding precursors o...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 16, 2009 Category: Biochemistry Authors: Wang M, Wang Y, Wang A, Song Y, Ma D, Yang H, Ma Y, Lai R Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Partial purification and characterization of trypsin-like proteinases from insecticide-resistant and -susceptible strains of the maize weevil, Sitophilus zeamais.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Serine proteinases from three strains of Sitophilus zeamais (Coleoptera: Curculionidae), one susceptible and two resistant to insecticides - one exhibiting fitness cost (resistant cost strain) and the other lacking it (resistant no-cost strain), were partially purified using an aprotinin-agarose affinity column providing purification factors ranging from 36.5 to 51.2%, with yields between 10 and 15% and activity between 529 and 875microM/min/mg protein with the substrate N-alpha-benzoyl-L-Arg-p-nitroanilide (L-BApNA). SDS-PAGE of the purified fraction revealed a 56,000 Da molecular mass band in all strains and a 70,000...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 12, 2009 Category: Biochemistry Authors: Silva LB, Reis AP, Pereira EJ, Oliveira MG, Guedes RN Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Acute phase protein haptoglobin in blood plasma samples of harbour seals (Phoca vitulina) of the Wadden Sea and of the isle Helgoland.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Haptoglobin (Hp) which is synthesized in response to infection, inflammation, trauma or toxicological damage is known as major acute phase protein in numerous species. Quantification of the circulating concentration of this protein can provide an objective measure of the health status, but there is a lack of investigations on harbour seals. We investigated the Hp concentration in samples of 123 seals (Phoca vitulina) from the German and Danish Wadden Sea to study physiological ranges of Hp levels. Hp levels between 2002, the end of the phocine distemper virus epidemic (PDV), and 2007 were considered, and Hp concentrati...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 5, 2009 Category: Biochemistry Authors: Kakuschke A, Erbsloeh HB, Griesel S, Prange A Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Tissue distribution of lipase genes related to triglyceride metabolism in laying hens (Gallus gallus).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we have identified and characterized the expression profile of these genes in the chicken, Gallus gallus. Chicken LPL and ATGL have been cloned, and HTGL, EL, Lipase H, and CEL sequences were found in the chicken genome database. The deduced amino acid sequences of HTGL, EL, Lipase H, and CEL were 66, 75, 63, and 65% identical with their respective human genes, suggesting conservation of important enzymatic functions. In contrast, a homologue of the HSL gene was not identified in the chicken genome. We performed RT-PCR using chicken liver, muscle, abdominal adipose tissue, or pancreas mRNA as the template, a...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 5, 2009 Category: Biochemistry Authors: Sato K, Seol HS, Kamada T Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Molecular characterization of a phospholipid-hydroperoxide glutathione peroxidase from the bumblebee Bombus ignitus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Phospholipid-hydroperoxide glutathione peroxidase (PHGPx or GPx4; EC 1.11.1.12) is an antioxidant enzyme that reduces lipid hydroperoxides in biomembranes. Here, we cloned and characterized cys-PHGPx from the bumblebee Bombus ignitus (Bi-PHGPx). The Bi-PHGPx gene consists of 4 exons, encoding 168 amino acid residues with a canonical cys-codon at residue 45 and active site residues Gln(82) and Trp(134). Recombinant Bi-PHGPx, expressed as a 19 kDa protein in baculovirus-infected insect cells, exhibited enzymatic activity against PLPC-OOH and H(2)O(2) using glutathione as an electron donor. Tissue distribution analyses sh...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - October 2, 2009 Category: Biochemistry Authors: Hu Z, Lee KS, Choo YM, Yoon HJ, Kim I, Wei YD, Gui ZZ, Zhang GZ, Sohn HD, Jin BR Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Impacts of dietary antioxidants and flight training on post-exercise oxidative damage in adult parrots.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
After intense physical activity animals generally experience a rise in metabolic rate, which is associated with a proliferation of pro-oxidants. If unchecked, these pro-oxidants can cause damage to DNA and peroxidation of lipids in cell walls. Two factors are thought to ameliorate post-exercise oxidative damage, at least in mammals: dietary antioxidants and exercise training. So far it is unknown whether birds benefit similarly from exercise training, although a positive effect of dietary antioxidants on take-off flight has been indicated. In this experiment, we maintained captive wildtype budgerigars Melopsittacus und...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 29, 2009 Category: Biochemistry Authors: Larcombe SD, Coffey JS, Bann D, Alexander L, Arnold KE Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Adipokine gene transcription level in adipose tissue of runt piglets.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Runt piglets were used as a model for neonatal stress to test the hypothesis that stress during the pre-weaning period can alter adipokine gene transcription levels. Runts were selected by birth mass <1kg and compared to littermates (controls) of mean litter weight. Subcutaneous (SQ) and perirenal (PR) adipose tissues were collected at d1 (n=5), d7 (n=7) or d21 (n=9) of age. Real-time PCR was used to quantify mRNA abundance for: leptin, adiponectin, interleukin 1beta (IL1beta), IL6, IL8, IL10, IL15, tumor necrosis factor alpha, haptoglobin, macrophage migration inhibitory factor (MIF), monocyte chemotactic protein, ...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 25, 2009 Category: Biochemistry Authors: Ramsay TG, Stoll MJ, Caperna TJ Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress: Effects on hemolymph and biochemical parameters.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Changes in water temperature and salinity are responsible for a variety of physiological stress responses in aquatic organisms. Stress induced by these factors was recently associated with enhanced reactive oxygen species (ROS) generation, which caused oxidative damage. In the present study, we investigated the time-related effects of changes in water temperature and salinity on mRNA expression and the activities of antioxidant enzymes (SOD and CAT) and lipid peroxidation (LPO) in the gills and digestive glands of the ark shell, Scapharca broughtonii. To investigate physiological responses, hydrogen peroxide (H(2)O(2))...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 25, 2009 Category: Biochemistry Authors: An MI, An KW, Choi CY Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Characterization of an ovary-specific glutathione peroxidase from the shrimp Metapenaeus ensis and its role in crustacean reproduction.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In vertebrates, both reactive oxygen species (ROS) and the corresponding scavenging system components especially glutathione peroxidase (GPx) are indispensible for normal development of the gonads. To investigate the function of GPx in crustaceans, we cloned and characterized a full length GPx (MeGPx) transcript in the penaeid shrimp Metapenaeus ensis. Phylogenetic analysis showed that MeGPx clustered with the GPx from mollusks and nematodes and shared much higher similarity with vertebrate GPx3 and GPx5 than with GPx1 or GPx2. Multiple sequence alignment further demonstrated that MeGPx is evolutionarily conserved amon...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 25, 2009 Category: Biochemistry Authors: Wu LT, Chu KH Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

IGF-I mediated inhibition of leptin receptor expression in porcine hepatocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A study was conducted to elucidate hormonal control of leptin receptor gene expression in primary cultures of porcine hepatocytes. Hepatocytes were isolated from swine and seeded into T-25 flasks. Cultures were established in medium containing fetal bovine serum for one day and switched to serum-free medium (William's E medium and 1ng/mL insulin) for the remainder of the three d culture period. For the final 24h, medium was supplemented with porcine growth hormone (GH, 100 or 500ng/mL), insulin-like growth factor 1 (IGF-1, 50 to 250ng/mL) or triiodothyronine (T3, 100ng/mL). RNA was extracted and relative quantitative R...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 24, 2009 Category: Biochemistry Authors: Ramsay TG, Richards MP, Li CJ, Caperna TJ Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Purification and partial characterization of a midgut membrane-bound alpha-glucosidase from Quesada gigas (Hemiptera: Cicadidae).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Adults of Quesada gigas (Hemiptera: Cicadidae) have a major alpha-glucosidase bound to the perimicrovillar membranes, which are lipoprotein membranes that surround the midgut cell microvilli in Hemiptera and Thysanoptera. Determination of the spatial distribution of alpha-glucosidases in Q. gigas midgut showed that this activity is not equally distributed between soluble and membrane-bound isoforms. The major membrane-bound enzyme was solubilized in the detergent Triton X-100 and purified to homogeneity by means of gel filtration on Sephacryl S-100, and ion-exchange on High Q and Mono Q columns. The purified alpha-gluc...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 16, 2009 Category: Biochemistry Authors: Fonseca FV, Silva JR, Samuels RI, Damatta RA, Terra WR, Silva CP Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

A Comparative Study of Ryanodine Receptor (RyR) Gene Expression Levels in a Basal Ray-Finned Fish, Bichir (Polypterus ornatipinnis) and the Derived Euteleost Zebrafish (Danio rerio).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ryanodine receptors (RyRs) are large homotetrameric protein complexes that mediate the release of intracellular stores of calcium. Mammals possess three gene copies, RyR1, RyR2, and RyR3 that are expressed in a variety of tissue types. Teleost fish express RyR1a and RyR1b genes that are expressed in slow twitch skeletal muscle and fast twitch skeletal muscles respectively. Here we report the results of a survey of the genome of bichir (Polypterus ornatipinnis), considered the most basal ray-finned fish, for its RyR genes. The bichir genome encodes four RyR genes, RyR1a, RyR1b, RyR2, and RyR3 that phylogenetically clust...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 11, 2009 Category: Biochemistry Authors: Darbandi S, Franck JP Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Effect of Monoclonal Antibody on Expression of Lipid Metabolism Related Genes in Porcine Adipocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In order to study the mechanism of monoclonal antibody (McAb) against a porcine 40-kDa adipocyte-specific plasma membrane protein in reducing fat deposition, porcine primary adipocytes were treated with the McAb during the process of adipocyte differentiation; its effect on expression of lipid metabolism related genes was investigated. Adipocytes were treated with 1-methyl-3-isobutylmethylxanthine (IDX) plus 10mug/mL of the McAb or without McAb. The mRNA levels of adipocyte differentiation related genes (PPARgamma and C/EBPalpha), lipid metabolism related genes (FAS, HSL, CPT-1B, DGAT and A-FABP) and adiponectin gene (...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 11, 2009 Category: Biochemistry Authors: Zhao SM, Wan QH, Cheng ML, Huang Y, Li WZ, Zhang YY, Gao SZ Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Stress-induced expression of protein disulfide isomerase associated 3 (PDIA3) in Atlantic salmon (Salmo salar L.).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In mammals, disulfide isomerase associated 3, PDIA3, is a member of the endoplasmic reticulum (ER) stress proteins, which can be induced by oxidative stress; however, its role in relation to stress regulation is still unknown in fish. Here, we report the cloning of a coding region of PDIA3 from the Atlantic salmon. PDIA3 mRNA expression was evaluated in the liver of Atlantic salmon exposed to environmental hyperoxia stress and toxic perfluorooctane sulfonate (PFOS) exposure stress. The PDIA3 sequence contained two PDI-typical thioredoxin active sites of WCGHC and shared approximately 70% identity with mammalian PDIA3, ...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 8, 2009 Category: Biochemistry Authors: Huang TS, Olsvik PA, Krøvel A, Tung HS, Torstensen BE Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Fasting suppresses T cell-mediated immunity in female Mongolian gerbils (Meriones unguiculatus).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Immune defense is important for organisms' survival and fitness. Small mammals in temperate zone often face seasonal food shortages. Generally fasting can suppress immune function in laboratory rodents and little information is available for wild rodents. The present study tested the hypothesis that Mongolian gerbils (Meriones unguiculatus) could inhibit T cell-mediated immunity to adapt to acute fasting. Forty-two females were divided into the fed and fasted groups, which the latter were deprived of food for 3days. After 66h fasting, half gerbils in each group were injected with phosphate buffered saline or phytohaema...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 6, 2009 Category: Biochemistry Authors: Xu DL, Wang DH Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Quercetin-metabolizing CYP6AS enzymes of the pollinator Apis mellifera (Hymenoptera: Apidae).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, quercetin was initially suggested as a shared substrate for CYP6AS1, CYP6AS3, and CYP6AS4, by its presence in honey, extracts of which induce transcription of these three genes, and by in silico substrate predictions based on a molecular model of CYP6AS3. Biochemical assays with heterologously expressed CYP6AS1, CYP6AS3, CYP6AS4 and CYP6AS10 enzymes subsequently confirmed their activity toward this substrate. CYP6AS1, CYP6AS3, CYP6AS4 and CYP6AS10 metabolize quercetin at rates of 0.47+/-0.10, 0.52+/-0.11, 0.20+/-0.04, and 0.22+/-0.10pmol quercetin/ pmol P450/ min, respectively. Substrate dockings and sequenc...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 4, 2009 Category: Biochemistry Authors: Mao W, Rupasinghe SG, Johnson RM, Zangerl AR, A Schuler M, Berenbaum MR Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

A novel tachykinin-like peptide from the skin of Xenopus laevis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Only one precursor of amphibian tachykinin has been characterized from the frog Odorrana grahami, although more than 30 tachykinins have been isolated from amphibians since 1964. The current result reports a second precursor of an amphibian tachykinin. A novel tachykinin-like peptide named tachykinin-Xl was identified from the skin of Xenopus laevis. The amino acid sequence was determined as PRPDQFYGLM-NH(2) with a conserved FXGLM-NH(2) C-terminal consensus motif. By cDNA cloning, the precursor of tachykinin-Xl was screened from the cDNA library of X. laevis skin, which was composed of 93 amino acid residues. The seque...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 31, 2009 Category: Biochemistry Authors: Yu H, Kong Y, Jia P, Zhang Z, Yan M, Zhang L, Liu J Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Cloning, expression, and pharmacological characterization of the GPR120 free fatty acid receptor from cynomolgus monkey: Comparison with human GPR120 splice variants.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Activation of the GPCR GPR120 by free fatty acids has been reported to cause GLP-1 release in rodent intestine. One genetic sequence was reported for rodents, while two sequences were reported for human GPR120, BC101175 and NM_181745. A 1086 base pair sequence cloned from cynomolgus monkey colon cDNA has 85.1% and 83.4% homology with the mouse and rat GPR120 sequences, and 97.5% homology with the human BC101175 sequence. No splice variants of the cynomolgus monkey GPR120 receptor were found. Eight non-synonymous cSNPs were discovered with frequencies less than 4% in monkey samples tested. Real-time PCR demonstrated tha...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 28, 2009 Category: Biochemistry Authors: Moore K, Zhang Q, Murgolo N, Hosted T, Duffy R Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Characterization of photoreceptor cell types in the little brown bat Myotis lucifugus (Vespertilionidae).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study provides important insights into the visual capacity of a nocturnal microchiropteran species. PMID: 19720154 [PubMed - as supplied by publisher] (Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology.)
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 27, 2009 Category: Biochemistry Authors: Feller KD, Lagerholm S, Clubwala R, Silver MT, Haughey D, Ryan JM, Loew ER, Deutschlander ME, Kenyon KL Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Tissue-specific transcriptional regulation of monocarboxylate transporters (MCTs) during short-term hypoxia in zebrafish (Danio rerio).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study has shown that fish utilize the tissue-specific transcriptional regulation of MCTs along with other metabolic genes during hypoxia. PMID: 19709642 [PubMed - as supplied by publisher] (Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology.)
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 22, 2009 Category: Biochemistry Authors: Ngan AK, Wang YS Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Main features of the oxidative metabolism in gills and liver of Odontesthes nigricans Richardson (Pisces, Atherinopsidae).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this work was to study comparatively oxidative metabolism in gills and liver of a silverside, Odontesthes nigricans, in their natural environment, the Beagle Channel. Oxidative damage to lipids was evaluated by assessing TBARS and lipid radical content, in gills and liver. Gills showed a significantly higher degree of damage than liver. The content of alpha-tocopherol, beta-carotene and catalase activity showed significantly higher values in the liver than in the gills. The ascorbyl radical (A(*)) content showed no significant differences between gills and liver. The ascorbate (AH(-)) content was 12+/-2 and ...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 21, 2009 Category: Biochemistry Authors: Lattuca ME, Malanga G, Hurtado CA, Pérez AF, Calvo J, Puntarulo S Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Transcriptional analysis of antioxidant and immune defense genes in disk abalone (Haliotis discus discus) during thermal, low-salinity and hypoxic stress.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study describes the transcriptional analysis of antioxidant and immune defense genes in gills tissue of disk abalone exposed to thermal, salinity and hypoxia-related stress, using quantitative real time PCR. Results showed that manganese superoxide dismutase (MnSOD), copper zinc superoxide dismutase (CuZnSOD), catalase (CAT), thioredoxin peroxidase (TPx), selenium dependant glutathione peroxidase (SeGPx), and thioredoxin-2 (TRx-2) transcripts were expressed differently in gills, and that they respond collectively as a classical enzymatic antioxidant defense system in abalone. Comparative analysis of expression profile...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 18, 2009 Category: Biochemistry Authors: De Zoysa M, Whang I, Lee Y, Lee JS, Lee J Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Volatile secretions and epicuticular hydrocarbons of the beetle Ulomoides dermestoides.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we characterized the volatile organic compounds (VOC) of the beetle Ulomoides dermestoides (Coleoptera: Tenebrionidae). Solid Phase Microextraction (SPME) coupled to Capillary Gas Chromatography - Mass Spectrometry (CGC-MS) analysis was used to identify methyl-1,4-benzoquinone (MBQ), ethyl-1,4-benzoquinone (EBQ), 1-tridecene (C(13:1)), and 1-pentadecene (C(15:1)), representing more than 90% of the volatile blend. We also used CGC-MS to analyze the epicuticular hydrocarbons of U. dermestoides. Saturated, unsaturated, and branched structures with chain lengths ranging from 13 to 43 carbons were detected. n-pent...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 14, 2009 Category: Biochemistry Authors: Villaverde ML, Girotti JR, Mijailovsky SJ, Pedrini N, Juárez MP Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Quantitative expression analysis of nacreous shell matrix protein genes in the process of pearl biogenesis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study examined the expression profiles of six genes encoding nacreous shell matrix proteins (NSMPs) in the pearl sac of aquaculture pearl oyster Pinctada fucata by real-time PCR. The comparative analysis of NSMP gene expression in the pearl sac and reference mantle tissues revealed that only a few NSMP genes maintained high transcript levels in the pearl sac. The impaired transcript levels of the nacrein gene refreshed the previous hypothesis, suggesting that CaCO(3) required for pearl secretion was not from pearl sac cells. Among the examined genes, only the N19 gene attained high expression levels in the pearl sac. ...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 13, 2009 Category: Biochemistry Authors: Wang N, Kinoshita S, Riho C, Maeyama K, Nagai K, Watabe S Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Differential ability of carotenoid C4-oxygenation in yellow and red bishop species (Euplectes spp.).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Male breeding plumages of African widowbirds and bishops (Euplectes spp.) show striking variation in carotenoid-based plumage coloration, with saturated yellow or orange-red patches of different size. Yet, from observations and experiments, agonistic signaling appears to have been a generalized sexual selection pressure for redness in the genus. Recent results show that yellow and red widowbird colors derive from distinctly different pigment profiles, and suggest that species vary in their ability to metabolize ingested carotenoids. We used reflectance spectrometry and High Performance Liquid Chromatography (HPLC) to d...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 13, 2009 Category: Biochemistry Authors: Prager M, Johansson EI, Andersson S Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Molluscan Shell Evolution with review of shell calcification hypothesis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study reconsiders the above three components with adding some biochemnical data of aculiferans. The presence of chitin in polyplacophorans sclerites was confirmed by IR and pyrolysis GC/MS. DMMB staining data inferred that sulphated groups present in aplacophoran cuticle but not in polyplacophorans cuticle. These insight suggested importance of comparison between acuriferans and conchiferans. PMID: 19665573 [PubMed - as supplied by publisher] (Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology.)
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 5, 2009 Category: Biochemistry Authors: Furuhashi T, Schwarzinger C, Miksik I, Smrz M, Beran A Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Dipeptidyl Peptidase IV Activity in the Blood of the American Alligator (Alligator mississippiensis).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Dipeptidyl peptidase IV (DPP4) enzyme activity, associated with cell surface and soluble CD26, was measured in alligator plasma and whole blood. DPP4 activity was higher in whole blood than in the plasma, presumably due to the inclusion of both membrane-bound CD26 on the surface of T-cells and sCD26 in the plasma. The plasma DPP4 activity was measured within 5 minutes after the addition of the substrate, and maximal accumulation of product was reached at 120 minutes. Alligator whole blood DPP4 activity peaked at 120 minutes. In addition, the DPP4 activity in both plasma and whole blood was temperature dependent, with m...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - August 5, 2009 Category: Biochemistry Authors: Merchant M, Monroe C, Falconi R Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Inhibition of spicule elongation in sea urchin embryos by the acetylcholinesterase inhibitor eserine.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this report, changes in activity and in the molecular differentiation of AchE were investigated. AchE activity increased slightly during gastrulation and rose sharply thereafter, and was dependent on new RNA synthesis. No activity of butyrylcholinesterase was found. Morphogenesis in sea urchin embryos was inhibited by the AchE inhibitor eserine, which specifically inhibited arm rod formation but not body rod formation. Spicule formation and enzyme activity in cultured micromeres were inhibited by eserine in a dose-dependent manner. During gastrulation, two molecular forms of AchE were detected with polyacrylamide gel el...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - June 28, 2009 Category: Biochemistry Authors: Ohta K, Takahashi C, Tosuji H Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Two metallothionein genes from mud loach Misgurnus mizolepis (Teleostei; Cypriniformes): gene structure, genomic organization, and mRNA expression analysis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Two metallothionein genes, MLMT-IA and MLMT-IB, were isolated and characterized from the mud loach Misgurnus mizolepis (Teleostei; Cypriniformes). For these MTs, we determined a tandem "tail-to-head" genomic organizational pattern, identified conserved genomic features, showed high sequence identities in the coding regions, and examined the closest phylogenetic affiliation, suggesting their divergence by a recent gene duplication event. However, the 5'-flanking upstream regions in MLMT-IA and MLMT-IB exposed large differences in the composition and distribution patterns of various transcription factor binding motifs, e...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - June 28, 2009 Category: Biochemistry Authors: Cho YS, Lee SY, Kim KY, Nam YK Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

The effect of feed restriction on expression of hepatic lipogenic genes in broiler chickens and the function of SREBP1.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study has helped us understand more about the function of SREBP1 and the physiology of the broiler chickens. PMID: 19383549 [PubMed - indexed for MEDLINE] (Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology.)
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - June 28, 2009 Category: Biochemistry Authors: Wang PH, Ko YH, Chin HJ, Hsu C, Ding ST, Chen CY Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Identification, mRNA expression and characterization of a novel ANK-like gene from Chinese mitten crab Eriocheir japonica sinensis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ankyrins are a family of adapter molecules mediating linkages between integral membrane and cytoskeletal proteins. Ankyrin repeat is one of the most frequently observed amino acid motifs in protein databases. A novel ANK-like gene of Chinese mitten crab Eriocheir japonica sinensis (denoted as EjsANK) was identified and cloned by expressed sequence tag and rapid amplification of cDNA end approaches. The full-length cDNA of EjsANK is 4375 bp and contains an open reading frame of 1095 bp which encodes a 364 amino acids polypeptide (40.23 kD) bearing seven ankyrin repeats. EjsANK cDNA has a 3073 bp uniquely long 3' untrans...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - June 28, 2009 Category: Biochemistry Authors: Li P, Zha J, Huang H, Zhang Z, Sun H, Song D, Zhou K Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Molecular cloning and characterization of porcine sirtuin genes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The sirtuin family of class III histone deacetylases (HDACs) is named after their homology to yeast silent information regulator 2 (SIR2). SIR2 and its mammalian derivatives (SIRT1-7) play a central role in gene silencing, cell cycle, aging and metabolism. Here we reported cDNA cloning, chromosome mapping,expression and evolutional analysis of sirtuin genes in Sus scrofa (Tongcheng pig). Sequence analysis showed that porcine sirtuin genes contain 7 members designated SIRT1-7. Tissue distribution analysis indicated porcine sirtuin genes ubiquitously expressed but with the highest abundance in brain, spinal cord and geni...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - June 28, 2009 Category: Biochemistry Authors: Jin D, Tan HJ, Lei T, Gan L, Chen XD, Long QQ, Feng B, Yang ZQ Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Functional differential immune responses of Mytilus galloprovincialis to bacterial challenge.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bivalves are filter-feeders that can accumulate large numbers of bacteria, in particular Vibrio species; these can persist within bivalve tissues largely depending on their sensitivity to the hemolymph bactericidal activity. In this work, functional parameters of the hemolymph of Mytilus galloprovincialis were evaluated in response to in vivo challenge with different bacteria (Gram(-) Vibrio anguillarum and V. splendidus, Gram+ Micrococcus lysodeikticus). Mussels were injected with heat-killed bacteria or PBS-NaCl (controls) and hemolymph sampled from 3 to 48 h post-injection (p.i.). In hemocytes, all bacteria induced ...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - June 28, 2009 Category: Biochemistry Authors: Ciacci C, Citterio B, Betti M, Canonico B, Roch P, Canesi L Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals

Bio-Mos: an effective inducer of dicentracin gene expression in European sea bass (Dicentrarchus labrax).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Concern over the use of dietary antibiotics in aquaculture has encouraged the industry to search for alternatives that both enhance performance and afford protection from disease. Bio-Mos, derived from the outer cell wall of a specific strain of yeast Saccharomyces cerevisiae (Alltech Inc, USA) is a product that fits these criteria. Here, we present data on the impact of a Bio-Mos supplemented diet on the mRNA copy number of the antimicrobial peptide dicentracin, whose transcript regulation has not yet been explored in fish.We analyzed Bio-Mos-induced changes in the expression of sea bass (Dicentrarchus labrax) dicentr...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - June 28, 2009 Category: Biochemistry Authors: Terova G, Forchino A, Rimoldi S, Brambilla F, Antonini M, Saroglia M Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: journals