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Efficacy of different protocols of vaccination against porcine circovirus type 2 (PCV2) in a farm affected by postweaning multisystemic wasting syndrome (PMWS).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In order to control growing problems associated with porcine circovirus type 2 (PCV2) several vaccines for piglets or sows were introduced recently. An objective of the study was to compare an efficacy of three different vaccination protocols in the herd with acute postweaning multisystemic wasting syndrome (PMWS) outbreak affecting 3-month-old pigs. All of three applied protocols, namely vaccination of sows, piglets or sows and piglets with Circovac proved to be efficacious in controlling of PMWS. All production parameters significantly improved after vaccination. Obtained values were as good as before the outbreak or...
Source: Comparative immunology, microbiology and infectious diseases. - November 11, 2009 Category: Allergy & Immunology Authors: Pejsak Z, Podgórska K, Truszczyński M, Karbowiak P, Stadejek T Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Development of rapid flow-through-based dot-immunoassay for serodiagnosis of leptospirosis in dogs.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An IgG-ELISA used recombinant antigen and a rapid flow-through enzyme immunoassay were developed for rapid screening of leptospiral antibodies in dogs using recombinant LipL41, which is one of the conserved outer membrane proteins in pathogenic leptospires as the coating antigen. Results from this study were compared with the standard microscopic agglutination test and found that the sensitivity and specificity of the enzyme-linked immunosorbent assay were 75.46% and 93.29% and whereas that of flow-through-based dot-immunobinding assay were 87.73% and 89.63%, respectively. Relative merits of these tests were also asses...
Source: Comparative immunology, microbiology and infectious diseases. - November 9, 2009 Category: Allergy & Immunology Authors: Subathra M, Senthilkumar TM, Ramadass P, Dhinakar Raj G Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Evaluation of safety and efficacy of DNA vaccines against bovine herpesvirus-1 (BoHV-1) in calves.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Four DNA vaccines against BoHV-1 were evaluated for their efficacy in calves. Twelve animals were divided into four groups which were injected with four different DNA vaccines: pVAX-tgD (Vaccine A); pVAX-tgD co-immunised with pVAX-48CpG (Vaccine B); pVAX-UbiLacI-tgD-L (Vaccine C); pVAX-UbiLacI-tgD-L co-immunised with pVAX-48CpG (Vaccine D). Three additional calves were given the plasmid vector and served as controls. Ninety days after the first vaccination all calves were challenge infected with BoHV-1. All animals developed a severe form of infections bovine rhinotracheitis. Only the calves given the pVAX-tgD co-immun...
Source: Comparative immunology, microbiology and infectious diseases. - November 9, 2009 Category: Allergy & Immunology Authors: Petrini S, Ramadori G, Corradi A, Borghetti P, Lombardi G, Villa R, Bottarelli E, Guercio A, Amici A, Ferrari M Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Development, characterization, and technical applications of a fish lysozyme-specific monoclonal antibody (mAb M24-2).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, a monoclonal antibody (mAb) for detecting and quantifying lysozyme was developed from mummichog, Fundulus heteroclitus, myeloid cells that also recognizes hen egg lysozyme (HEL), then tested for cross-reactivity in different species of teleosts. A single protein of approximately 14-15kDa mass was identified by the mAb in fish cell lysates and plasma samples, as well as denatured HEL. Total circulating lysozyme protein was compared to lysozyme activity using standard ELISA procedures and was found to correlate with enzymatic activity. Using mAb M24-2, intracellular lysozyme protein was detected in formalin-fi...
Source: Comparative immunology, microbiology and infectious diseases. - November 7, 2009 Category: Allergy & Immunology Authors: Marsh MB, Rice CD Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Acute phase protein changes in calves during an outbreak of respiratory disease caused by bovine respiratory syncytial virus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, APPs (especially SAA and LBP) are sensitive markers of respiratory infection, and they may be useful to explore host response to the respiratory infections in clinical research. PMID: 19897247 [PubMed - as supplied by publisher] (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - November 6, 2009 Category: Allergy & Immunology Authors: Orro T, Pohjanvirta T, Rikula U, Huovilainen A, Alasuutari S, Sihvonen L, Pelkonen S, Soveri T Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Phylogenetic analysis of recent classical swine fever virus (CSFV) isolates from Assam, India.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Classical swine fever (CSF), a highly contagious viral disease of pigs, is endemic in India. As there is no information concerning the accurate genetic typing of classical swine fever virus (CSFV) isolates in India, 16 CSF viruses isolated during 2005-2007 from domestic pigs in different districts of Assam were typed in 5' UTR (150 nucleotides). To confirm the genetic typing results and to study the genetic variability, selected viruses were also analyzed in E2 (190nt) and NS5B gene (409nt) regions. Phylogenetic analysis revealed that all the 16 CSFV isolates analyzed belonged to group 1 and subgroup 1.1 in contrast to...
Source: Comparative immunology, microbiology and infectious diseases. - November 5, 2009 Category: Allergy & Immunology Authors: Sarma DK, Mishra N, Vilcek S, Rajukumar K, Behera SP, Nema RK, Dubey P, Dubey SC Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Effect of FMD vaccine antigen payload on protection, sub-clinical infection and persistence following needle challenge in sheep.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The relationship of Foot-and-Mouth Disease (FMD) virus antigen payload and single and double vaccinations in conferring protection against virus challenge in sheep was studied. Sheep vaccinated with half the cattle dose (1ml) containing 15 and 3.75mug of FMDV antigen with or without booster resisted virulent challenge on 21 days post vaccination or 7 days post booster. FMDV RNA could be detected in nasal secretions in 26% of vaccinated sheep (10(3.12) to 10(3.82) viral RNA copies) on day 35 post challenge. No live virus could be isolated after 5 days post challenge indicating that the risk of transmission of disease wa...
Source: Comparative immunology, microbiology and infectious diseases. - November 5, 2009 Category: Allergy & Immunology Authors: Madhanmohan M, Nagendrakumar SB, Narasu ML, Srinivasan VA Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Immunomodulation of caprine lentiviral infection by interleukin-16.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we determined the immunomodulatory effects of IL-16 in vitro using cells derived from CAEV infected and uninfected goats. Human recombinant IL-16 (rhIL-16) significantly increased chemotaxis of peripheral blood mononuclear cells (PBMCs) of both control and CAEV-infected goats. Pretreatment of PBMC with anti-goat CD4 monoclonal antibody inhibited IL-16-induced chemotaxis of PBMC of control and infected goats suggesting that IL-16 exerts its action in goats primarily by binding to CD4. The CAEV proviral DNA was less in caprine monocytes treated with rhIL-16 infected in vitro with CAEV. These data suggest inhib...
Source: Comparative immunology, microbiology and infectious diseases. - October 5, 2009 Category: Allergy & Immunology Authors: Nimmanapalli R, Sharmila C, Reddy PG Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Chlamydia trachomatis evokes a relative anti-inflammatory response in a free Ca(2+) dependent manner in human macrophages.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chlamydia trachomatis infections manifest as unique, chronic inflammatory diseases, indicating a relative compromise in the capacity of early immune responders such as macrophages to resolve the infection. We decided to investigate whether or not the chronic inflammatory manifestations are influenced by a disturbance in the pattern of inflammatory:anti-inflammatory cytokine elaboration early in the infection cycle in macrophages and assess the possible modulatory role of Ca(2+) signals in the process. Although the basal and functional levels of IL-12 and IL-10 are not identical in concentration, chlamydia initiated a s...
Source: Comparative immunology, microbiology and infectious diseases. - September 23, 2009 Category: Allergy & Immunology Authors: Azenabor AA, York J Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

DNA vaccine encoding avian influenza virus H5 and Esat-6 of Mycobacterium tuberculosis improved antibody responses against AIV in chickens.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The H5 gene of avian influenza virus (AIV) strain A/chicken/Malaysia/5744/2004(H5N1) was cloned into pcDNA3.1 vector, and Esat-6 gene of Mycobacterium tuberculosis was fused into downstream of the H5 gene as a genetic adjuvant for DNA vaccine candidates. The antibody level against AIV was measured using enzyme-linked immunosorbent assay (ELISA) and haemagglutination inhibition (HI) test. Sera obtained from specific-pathogen-free chickens immunized with pcDNA3.1/H5 and pcDNA3.1/H5/Esat-6 demonstrated antibody responses as early as 2 weeks after the first immunization. Furthermore, the overall HI antibody titer in chicke...
Source: Comparative immunology, microbiology and infectious diseases. - September 22, 2009 Category: Allergy & Immunology Authors: Oveissi S, Omar AR, Yusoff K, Jahanshiri F, Hassan SS Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Nitric oxide and lysozyme production as an impact to Clostridium perfringens mastitis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study is, to our knowledge, the first to suggest a possible link between NO and lysozyme and bovine mastitis caused by C. perfringens. The results raise the possibility that interfering with NO production during mastitis may help to prevent tissue damage. PMID: 19783303 [PubMed - as supplied by publisher] (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - September 21, 2009 Category: Allergy & Immunology Authors: Osman KM, El-Enbaawy MI, Ezzeldin NA, Hussein HM Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

The use of MPB70 and MPB83 to distinguish between bovine tuberculosis and paratuberculosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In order to demonstrate the potential to distinguish paratuberculosis (PTB) from bovine tuberculosis infection (TB), ELISAs with M. bovis-specific MPB70 or MPB83 as capture antigens were developed and tested on two groups of cattle: Group A comprised 23 animals positive for Mycobacterium avium paratuberculosis (Map) and TB free. Group B comprised 48 animals from a Map free herd during the previous 5 years, but confirmed as tuberculous by positive results on PPD testing and M. bovis culture. Results demonstrated a significant difference (p<0.01) between reactivity of sera from these groups, encouraging the study of p...
Source: Comparative immunology, microbiology and infectious diseases. - September 4, 2009 Category: Allergy & Immunology Authors: Marassi CD, McNair J, Pollock J, Ristow P, Fonseca LS, Oelemann WM, Lilenbaum W Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Detection of all Chlamydophila and Chlamydia spp. of veterinary interest using species-specific real-time PCR assays.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of the present study was to analyse the occurrence of chlamydiae in several mammalian host species. Clinical samples that previously tested positive in a Chlamydiaceae-specific real-time PCR were retested using six species-specific real-time PCR assays to identify the chlamydial species involved. Chlamydophila (Cp.) abortus was the agent most frequently found in cattle, sheep, horses, goats, and pigs. Detection in cattle of Cp. psittaci (11% of samples) and Chlamydia (C.) suis (9%), as well as Cp. psittaci in a goat sample was somewhat unexpected. DNA of two different chlamydiae was identified in 56 (12.7%) of ...
Source: Comparative immunology, microbiology and infectious diseases. - September 3, 2009 Category: Allergy & Immunology Authors: Pantchev A, Sting R, Bauerfeind R, Tyczka J, Sachse K Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Effect of Momordica charantia L. in the resistance to aminoglycosides in methicilin-resistant Staphylococcus aureus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study the ethanol extract (EEMC) of Momordica charantia L. (Cucurbitaceae) was tested for its modifying antibiotic activity against a MRSA strain. The growth of an MRSA (SA358) in the absence and presence of aminoglycosides was evaluated. A potentiating effect between this extract and all aminoglycosides was demonstrated. Similarly, the same effect was shown by chlorpromazine on kanamycin, gentamicin and neomycin, indicating the involvement of an efflux system in the resistance to these aminoglycosides. Extracts from M. charantia could be used as a source of plant-derived natural products with resistance-modifying ...
Source: Comparative immunology, microbiology and infectious diseases. - September 1, 2009 Category: Allergy & Immunology Authors: Coutinho HD, Costa JG, Falcão-Silva VS, Siqueira-Júnior JP, Lima EO Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Innate immune responses of primary murine macrophage-lineage cells and RAW 264.7 cells to ligands of Toll-like receptors 2, 3, and 4.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Although studies have been performed to characterize responses of macrophages from individual anatomical sites (e.g., alveolar macrophages) or of murine-derived macrophage cell lines to microbial ligands, few studies compare these cell types in terms of phenotype and function. We directly compared the expression of cell surface markers and functional responses of primary cultures of three commonly used cells of monocyte-macrophage lineage (splenic macrophages, bone marrow-derived macrophages, and bone marrow-derived dendritic cells) with those of the murine-leukemic monocyte-macrophage cell line, RAW 264.7. We hypothes...
Source: Comparative immunology, microbiology and infectious diseases. - September 1, 2009 Category: Allergy & Immunology Authors: Berghaus LJ, Moore JN, Hurley DJ, Vandenplas ML, Fortes BP, Wolfert MA, Boons GJ Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

The role of Brucellacapt test for follow-up patients with brucellosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The dynamic of Brucellacapt titers was evaluated in 104 patients with brucellosis with favorable outcome and in 28 patients with persistent illness duration, during the follow-up period of 15 months. In patients with favorable outcome, a permanently decreasing tendency of Brucellacapt titers was evident. Titers</=1/320 were noted in 27% and 90% of the patients, at the end of the 4th and 15th month, respectively. In patients with persistent disease, persistence or slow titre regression during the entire follow-up period was evident. Four and 15 months of the treatment, titers of 1/320 were registered in 4%, and 14%, ...
Source: Comparative immunology, microbiology and infectious diseases. - July 19, 2009 Category: Allergy & Immunology Authors: Bosilkovski M, Katerina S, Zaklina S, Ivan V Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Highly pathogenic H5N1 avian influenza virus: cause of the next pandemic?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Since 1997, when human infections with a highly pathogenic (HP) avian influenza A virus (AIV) subtype H5N1 - previously infecting only birds - were identified in a Hong Kong outbreak, global attention has focused on the potential for this virus to cause the next pandemic. From December 2003, an unprecedented H5N1 epizootic in poultry and migrating wild birds has spread across Asia and into Europe, the Middle East, and Africa. Humans in close contact with sick poultry and on rare occasion with other infected humans, have become infected. As of early March 2007, 12 countries have reported 167 deaths among 277 laboratory-...
Source: Comparative immunology, microbiology and infectious diseases. - April 6, 2009 Category: Allergy & Immunology Authors: Pappaioanou M Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Synthetic peptide issued from Hap1/LipL32 for new early serodiagnosis of human leptospirosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Leptospirosis is a worldwide zoonosis. Today, serological diagnosis is generally assessed by MAT. We performed ELISA with a synthetic peptide derived from Hap1/lipL32 which is a protein expressed only by pathogenic Leptospira. Repeatability and thresholds were defined with 85 controls sera and 119 hospitalized leptospirosis. The PP-ELISA repeatability and IgM/IgG cut-off values were based on control sera. For these cut-off values, we observed the IgM-PP-ELISA specificity of 89%, whereas it was 100% for the IgG. Then, we compared PP-ELISA and standard MAT results for leptospirosis patients. The concordance rate for IgM-...
Source: Comparative immunology, microbiology and infectious diseases. - March 21, 2009 Category: Allergy & Immunology Authors: Aviat F, Rochereau-Roulet S, Branger C, Estavoyer JM, Chatrenet B, Orsonneau JL, Thorin C, Andre-Fontaine G Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Expression of biologically active recombinant equine interferon-gamma in Escherichia coli.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this report, the gene coding equine IFN-gamma (EIFN-gamma) mature protein was cloned into pET-28a (+) and the recombinant EIFN-gamma was expressed in Escherichia coli (E. coli). The antiviral activity of expressed recombinant EIFN-gamma was evaluated by using a recombinant Vesicular Stomatits Virus expressing green fluorescence protein (rVSV-GFP) system in the equine fetal kidney-78 cell line (EFK-78). The GFP expression in the EFK-78 cells dramatically decreased in the cells treated with EIFN-gamma in a dose-dependent manner, comparing with the mock-treated cells. The titer of antiviral activity was 1x10(3)AU/ml. These...
Source: Comparative immunology, microbiology and infectious diseases. - March 9, 2009 Category: Allergy & Immunology Authors: Bai Y, Tong T, Liu G, Chen W, Zhang W, Wang Q, Yang T, Bu Z, Wu D Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Virological and antigenic characterization of two Peste des Petits Ruminants (PPR) vaccine viruses of Indian origin.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Virological and antigenic characteristics of two Indian Peste des Petits Ruminants (PPR) vaccine viruses namely "PPRV-Sungri/96" and "PPRV-AR/87" were investigated. This investigation included, type of cytopathic effect (CPE) produced, one-step growth curve, antigenic reactivity of viral antigens using a panel of 18 monoclonal antibodies (MAbs) and ability of viruses for neutralization using a monoclonal antibody directed to haemagglutinin (H) protein. Findings surprisingly indicated that the PPRV-AR/87 is a fast growing virus with an entirely different pattern of cytopathic effect in Vero cell system. This virus has a...
Source: Comparative immunology, microbiology and infectious diseases. - February 4, 2009 Category: Allergy & Immunology Authors: Singh RP, De UK, Pandey KD Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Prevalence of virulence-associated genes and cytolethal distending toxin production in Campylobacter spp. isolated in Italy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The prevalence of virulence and cytolethal distending toxin (CDT) genes and the cytotoxic activity in Vero and HEp-2 cells was estimated in 29 Campylobacter jejuni and 36 Campylobacter coli from foods, animals and humans isolates. All C. jejuni showed flaA, cadF, cdtA, cdtB, cdtC and cdt cluster genes fragments, except for ceuE (86.2%) and cdt genes (93.1%). Amongst C. coli strains, a lower prevalence of ceuE gene (83.3%) was detected than that for cdtA, cdtB, cdtC genes (97.2%), cdt gene cluster (94.4%) and cdt genes (86.1%); whereas flaA and cadF genes were amplified in all isolates. Despite the high prevalence of CD...
Source: Comparative immunology, microbiology and infectious diseases. - February 3, 2009 Category: Allergy & Immunology Authors: Ripabelli G, Tamburro M, Minelli F, Leone A, Sammarco ML Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Genetic characterization of H5N1 influenza virus that caused new outbreak in Israel at the beginning of 2008.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Our aim was to characterize the A/ck/Israeli/1055/2008 (H5N1) avian influenza virus that was isolated at the beginning of 2008, and to establish the phylogenetic relationship of this isolate to other H5N1 viruses that were recently isolated in adjacent countries. In light of a study of complete nucleotide sequences of all the genes we found that the isolate (year 2008) was closely related to the H5N1 viruses isolated in Egypt, Israel and Gaza in 2006. The Israeli isolate had the hemagglutinin-connecting peptide with a polybasic amino acid insertion. The most host-restriction sites of the 2008 isolate were typical of av...
Source: Comparative immunology, microbiology and infectious diseases. - February 2, 2009 Category: Allergy & Immunology Authors: Shimon P, Golender N, Lapin E, Pokamunski S, Berman E, Tendler Y, Bellaiche M, Panshin A, Davidson I Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Comparative analysis of four lipoproteins from Mycoplasma mycoides subsp. mycoides Small Colony identifies LppA as a major T-cell antigen.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Control of contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), remains an important goal in Africa. Subunit vaccines triggering B and T-cell responses could represent a promising approach. To this aim, the T-cell immunogenicity of four MmmSC lipoproteins (LppA, LppB, LppC and LppQ), present in African strains and able to elicit humoral response, was evaluated. In vitro assays revealed that only LppA was recognized by lymph node lymphocytes taken from three cattle, 3 weeks after MmmSC exposure. Maintenance of the LppA-specific response, relying on CD4 T-cells and...
Source: Comparative immunology, microbiology and infectious diseases. - January 31, 2009 Category: Allergy & Immunology Authors: Dedieu L, Totte P, Rodrigues V, Vilei EM, Frey J Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Down-regulatory effect of alpha(1)-acid glycoprotein on bovine neutrophil degranulation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this paper, the possible involvement of the acute phase protein alpha(1)-acid glycoprotein (AGP) in the local immunomodulation of inflammation was investigated. The dose response of bovine neutrophils to AGP as to mobilization of primary and secondary granules was studied. It was found that AGP fulfils a protective role against spontaneous exocytosis of secondary, but not primary, granules. This downregulatory effect is much more evident when degranulation is challenged with Zymosan activated serum (ZAS). AGP activity is dose-dependent, the acute phase concentration being more active than the physiological one. Carb...
Source: Comparative immunology, microbiology and infectious diseases. - January 22, 2009 Category: Allergy & Immunology Authors: Miranda-Ribera A, Lecchi C, Bronzo V, Scaccabarozzi L, Sartorelli P, Franciosi F, Ceciliani F Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Acute-phase protein response in pigs experimentally infected with Haemophilus parasuis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, APP response can reflect Glässer-disease ongoing, showing a correlation between the severity and duration of the clinical signs and lesions and the magnitude of changes in the APP levels. PMID: 19117607 [PubMed - as supplied by publisher] (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - December 29, 2008 Category: Allergy & Immunology Authors: Martín de la Fuente AJ, Carpintero R, Rodríguez Ferri EF, Alava MA, Lampreave F, Gutiérrez Martín CB Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Prevalence of Q fever in domestic animals with reproductive disorders.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The occurrence of Coxiella burnetii in animals with reproductive disorders was studied. A total of 920 samples (genital and faecal swabs, milk, urine and serum) were collected from cows (88), buffaloes (33), sheep (43) and goats (53) with a history of reproductive disorders and screened for C. burnetii by a PCR assay targeting the repetitive transposon-like region of C. burnetii (trans-PCR), real-time PCR, indirect immunofluorescence assay (IFA), enzyme-linked immunosorbent assay (ELISA) and isolation method. The overall prevalence of Q fever in animals with the history of reproductive disorders turned out to be 13.82%...
Source: Comparative immunology, microbiology and infectious diseases. - December 18, 2008 Category: Allergy & Immunology Authors: Vaidya VM, Malik SV, Bhilegaonkar KN, Rathore RS, Kaur S, Barbuddhe SB Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Identification of full-length proviral DNA of porcine endogenous retrovirus from Chinese Wuzhishan miniature pigs inbred.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We described here the cloning and characterization of full-length proviral DNA of PERV from Chinese Wuzhishan miniature pigs inbred (WZSP). Full-length nucleotide sequences of PERV-WZSP and other PERVs were aligned and phylogenetic tree was constructed from deduced amino-acid sequences of env. The results demonstrated that the full-length proviral DNA of PERV-WZSP belongs to gammaretrovirus and shares high similarity with other PERVs. Sequence analysis also suggested that different patterns of LTR existed in the same porcine germ line and partial PERV-C sequence may recombine with PERV-A sequence in LTR. PMID: 19070900...
Source: Comparative immunology, microbiology and infectious diseases. - December 12, 2008 Category: Allergy & Immunology Authors: Ma Y, Lv M, Xu S, Wu J, Tian K, Zhang J Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

High seroprevalence of bluetongue virus infection in sheep flocks in West Azerbaijan, Iran.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bluetongue (BT) is an important disease of ruminant livestock that is currently emerging in previously unaffected regions, most notably extensive portions of Europe. The epidemiology of BT virus (BTV) infection is poorly defined in much of the world, including extensive portions of Asia and the Middle East. Thus, the objective of this study was to describe the distribution and seroprevalence of BTV infection of sheep in West Azerbaijan Province of Iran, and to identify demographic and climatological factors associated with exposure of these sheep to BTV infection. BTV infection was widespread in the province as 400 of ...
Source: Comparative immunology, microbiology and infectious diseases. - December 5, 2008 Category: Allergy & Immunology Authors: Shoorijeh SJ, Ramin AG, Maclachlan NJ, Osburn BI, Tamadon A, Behzadi MA, Mahdavi M, Araskhani A, Samani D, Rezajou N, Amin-Pour A Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Multi-host pathogens and carnivore management in southern Africa.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A retrospective serosurvey of multi-host feline and canine viruses among carnivore species in southern Africa (n=1018) identified widespread pathogen exposure even in remote protected areas. In contrast to morality experienced in East African predators, canine distemper virus (CDV) infection among African wild dogs (Lycaon pictus) in Botswana was not associated with identifiable change in pup survivorship or disease related mortality of adults. A disease outbreak of unknown aetiology occurred in the same population over 4 weeks in 1996. Outbreak boundaries coincided with ecotones, not the spatial distribution of contig...
Source: Comparative immunology, microbiology and infectious diseases. - November 25, 2008 Category: Allergy & Immunology Authors: Alexander KA, McNutt JW, Briggs MB, Standers PE, Funston P, Hemson G, Keet D, van Vuuren M Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Host-virus specificity of morbilliviruses predicted by structural modeling of the marine mammal SLAM, a receptor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Signaling lymphocyte activation molecule (SLAM) is thought to be a major cellular receptor for high-host specificity morbilliviruses, which cause devastating and highly infectious diseases in mammals. We determined the sequences of SLAM cDNA from five species of marine mammal, including two cetaceans, two pinnipeds and one sirenian, and generated three-dimensional models to understand the receptor-virus interaction. Twenty-one amino acid residues in the immunoglobulin-like V domains of the SLAMs were shown to bind the viral protein. Notably, the sequences from pinnipeds and dogs were highly homologous, which is consist...
Source: Comparative immunology, microbiology and infectious diseases. - November 21, 2008 Category: Allergy & Immunology Authors: Ohishi K, Ando A, Suzuki R, Takishita K, Kawato M, Katsumata E, Ohtsu D, Okutsu K, Tokutake K, Miyahara H, Nakamura H, Murayama T, Maruyama T Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Differences between Pseudomonas aeruginosa in a clinical sample and in a colony isolated from it: Comparison of virulence capacity and susceptibility of biofilm to inhibitors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We study the differences between Pseudomonas aeruginosa from an infected wound (clinical strain) and a colony isolated from it. We assessed the in vitro inhibition of these P. aeruginosa biofilms by DNase and filtrate of Lactobacillus plantarum cultures (acid=AF and neutralize=NF) with crystal violet technique. Inhibition by AF was greatest than DNase for clinical and isolated strain (p<0.001) and greatest than NF for clinical (p<0.05) and isolated strain (p<0.001). Using a burn model in mice, we compared the infection producing by clinical and isolated strains in planktonic and biofilm form. Deaths were quant...
Source: Comparative immunology, microbiology and infectious diseases. - November 21, 2008 Category: Allergy & Immunology Authors: Ramos AN, Peral MC, Valdez JC Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Immune response against adhesins of enteroaggregative Escherichia coli immunized by three different vaccination strategies (DNA/DNA, Protein/Protein, and DNA/Protein) in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Enteroaggregative Escherichia coli (EAEC) are an increasingly recognized enteric pathogen. It is a cause of both acute and persistent diarrhoea among children, adults and HIV-infected persons, in both developing and developed countries. The aggregative adherence of EAEC is due to the presence of aggregative adherence fimbriaes (AAFs). To elucidate the possible protective role of these adhesins in diarrheagenic E. coli with DNA immunization approach, Balb/c mice were immunized with three different modes of vaccination, i.e. DNA/DNA, DNA/Protein, or Protein/Protein of Aggregative Adherence Factors, AAF/I or AAF/II of ent...
Source: Comparative immunology, microbiology and infectious diseases. - November 18, 2008 Category: Allergy & Immunology Authors: Bouzari S, Dashti A, Jafari A, Oloomi M Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Toll-like receptor expressions in porcine alveolar macrophages and dendritic cells in responding to poly IC stimulation and porcine reproductive and respiratory syndrome virus (PRRSV) infection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Antigen-presenting cells play critical roles in recognizing, presenting and processing antigens and consequently induce adequate immune response for defending infections. The immature DCs (imDCs) and mature DCs (mDCs) were obtained from in vitro differentiation of bone marrow haematopoietic cells. Results showed that poly IC stimulation down-regulated the expressions of TLR7 and TLR8 in alveolar macrophages (AMs) and imDCs. The release of IL-12 was inhibited from imDCs and mDCs in response to poly IC. Porcine reproductive and respiratory syndrome virus (PRRSV)-infection inhibited TLR3 and TLR7 expressions in AMs and im...
Source: Comparative immunology, microbiology and infectious diseases. - November 11, 2008 Category: Allergy & Immunology Authors: Chaung HC, Chen CW, Hsieh BL, Chung WB Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Inhibition of host protein synthesis in B95a cells infected with the HL strain of measles virus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this report, we showed that the HL strain of measles virus (MeV-HL) as well as other field isolates, which were isolated from human blood lymphocytes using B95a cells, induce the shut-off in B95a cells. Since the Edmonston strain of MeV failed to induce the shut-off in B95a cells, the ability to induce the shut-off was considered to be dependent on virus strains. Although, the modification of eukaryotic translation initiation factors (eIF) including eIF4G, eIF4E, and 4E-BP1 was reported for shut-off by various viruses, the involvement of these eIFs was not observed in MeV-HL-infected B95a cells. Instead, the accumulatio...
Source: Comparative immunology, microbiology and infectious diseases. - October 17, 2008 Category: Allergy & Immunology Authors: Inoue Y, Tsukiyama-Kohara K, Yoneda M, Sato H, Kai C Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Mannan-binding lectin (MBL) in two chicken breeds and the correlation with experimental Pasteurella multocida infection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study is the first demonstration of an association of the genetic serum Mannan-binding lectin (MBL) concentration with bacterial infections in chickens. The genetic serum MBL concentration was determined in two chicken breeds, and the association with the specific Pasteurella multocida humoral immune response during an experimental infection was examined. Furthermore, we examined the association of the genetic serum MBL concentration with systemic infection. The chickens with systemic infection had a statistically significant lower mean serum MBL concentration than the rest of the chickens, suggesting that ...
Source: Comparative immunology, microbiology and infectious diseases. - October 13, 2008 Category: Allergy & Immunology Authors: Schou TW, Permin A, Christensen JP, Cu HP, Juul-Madsen HR Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Seroprevalence of Q fever (coxiellosis) in sheep from the Southern Marmara Region, Turkey.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed at investigating the seroprevalence in sheep flocks from three provinces (Bursa, Balikesir and Canakkale). Serosurvey was undertaken on 42 flocks, which were categorised by sizes. Sera were collected randomly from specific age groups within the young population. CHEKIT Q-fever ELISA kit was used to identify the infection in sheep. The results showed that 20% (n=151) of sheep were seropositive. A total of 34 flocks (81%) revealed at least one seropositive animal. Higher seroprevalence was observed in Balikesir region. Larger flocks resulted more infected than medium and small flocks. An association was foun...
Source: Comparative immunology, microbiology and infectious diseases. - October 8, 2008 Category: Allergy & Immunology Authors: Kennerman E, Rousset E, Gölcü E, Dufour P Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

The impact of staphylococcal mastitis on the level of milk IL-6, lysozyme and nitric oxide.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mammary gland secretions derived from secretory cows infected with coagulase +ve Staphylococcus spp. was examined for the expression of IL-6, production of lysozyme and NO(x). The examined cows reflected 25 cases of subclinical mastitis and 15 cases of clinically mastitic animals. The IL-6 concentration in the subclinical animals was significantly higher (30.8ng/ml) than the clinically manifested animals (18.0ng/ml) and the normal cows (5.2ng/ml). On the other hand the level of lysozyme although significantly higher than the normal cows (6.9mug/ml) yet its level in the subclinical animals (11.2mug/ml) was lower than th...
Source: Comparative immunology, microbiology and infectious diseases. - September 30, 2008 Category: Allergy & Immunology Authors: Osman KM, Hassan HM, Ibrahim IM, Mikhail MM Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Immune system and thiols: Some peculiarities of thiol exchange.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We show that for the system of SH-containing compounds of the organism (cysteine, in particular) there are conditions leading to immunosuppression, which occurs when the level of amino acid cysteine in blood serum increases. The arising "overload" of free sulfhydryl groups inactivates antibodies of the IgM class of any specificity restoring disulphide intramolecular bonds and then the number of immune antibody-producing cells, mitotic activity of T- and B-lymphocytes and interleukin-2 synthesis are lowered and the mobility of surface immunoglobulin B lymphocytes receptors is damaged. Determination of total blood sulfhy...
Source: Comparative immunology, microbiology and infectious diseases. - September 24, 2008 Category: Allergy & Immunology Authors: Rodosskaia NK, Chernousova GM Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Effect of polymyxin B and environmental conditions on isolation of Brucella species and the vaccine strain RB51.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Brucella are resistant to polymyxin B (PB), but their relative susceptibility to PB and its derivative, colistin (COL) has not been rigorously or systematically studied. Comparative susceptibility of Brucella reference strains, vaccine strain RB51, and Brucella isolates from marine mammals to these two cationic peptides were determined by Etest. Vast differences among Brucella species were found in susceptibility to both PB and COL. Brucella demonstrated similar pattern of relative susceptibility to PB as that of COL, but they were less susceptible to COL. Both B. melitensis and B. suis were the least susceptible to po...
Source: Comparative immunology, microbiology and infectious diseases. - September 22, 2008 Category: Allergy & Immunology Authors: Jensen AE, Halling SM Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Molecular typing and antimicrobial resistance of Salmonella enterica subspecies enterica serovar Choleraesuis isolates from diseased pigs in Japan.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Salmonella enterica serovar Choleraesuis (S. Choleraesuis) isolates derived from diseased pigs in Japan during 2001 and 2005 were analyzed for biotype, based on H(2)S production and dulcitol fermentation, pulsed-field gel electrophoresis (PFGE) profile, and antimicrobial resistance profile. S. Choleraesuis biotype Choleraesuis (biotype Choleraesuis) was classified into one genotype, while varietas Kunzendorf (var. Kunzendorf) was classified into two genotypes. The isolates of var. Kunzendorf belonging to one genotype were isolated in a limited area of Japan. Variation in the antimicrobial resistance pattern was observe...
Source: Comparative immunology, microbiology and infectious diseases. - September 20, 2008 Category: Allergy & Immunology Authors: Asai T, Namimatsu T, Osumi T, Kojima A, Harada K, Aoki H, Sameshima T, Takahashi T Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Anaplasma phagocytophilum in horses and ticks: A preliminary survey of Central Italy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, affects several species of wild and domesticated mammals, including horses. In this work we compared direct and indirect methods to evaluate A. phagocytophilum presence in Central Italy: 135 sera were screened by IFA for A. phagocytophilum and other haemopathogens (Theileria equi and Babesia caballi). Each horse was also tested for A. phagocytophilum 16S rRNA with a nested-PCR technique. In order to examine the risk of A. phagocytophilum transmission, 114 ticks were examined for the presence of A. phagocytophilum by PCR targeting the 16S rRNA....
Source: Comparative immunology, microbiology and infectious diseases. - September 19, 2008 Category: Allergy & Immunology Authors: Passamonti F, Fabrizia V, Katia C, Stefano C, Giacomo C, Luisa MM, Daniela PF, Andrea VS, Mauro C Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Wildlife (Boselaphus tragocamelus)-small ruminant (goat and sheep) interface in the transmission of 'Bison type' genotype of Mycobacterium avium subspecies paratuberculosis in India.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Information on Mycobacterium avium subspecies paratuberculosis (MAP) genotypes infecting different animal species in India is limited. Presence of MAP was investigated in free ranging antelopes (locally known as Nilgai/blue bulls/Boselaphus tragocamelus) using direct microscopy, culture, IS900 PCR and IS1311 PCR-REA. IS900 elements of MAP from Nilgai and previously isolated from goats were sequenced and compared to establish inter-species transmission between free ranging Nilgai and closed farm herds and flocks of goats and sheep sharing common grazing and water resources. Fecal samples were collected from two geograph...
Source: Comparative immunology, microbiology and infectious diseases. - September 19, 2008 Category: Allergy & Immunology Authors: Kumar S, Singh SV, Singh AV, Singh PK, Sohal JS, Maitra A Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Production of monoclonal antibodies specific to major outer membrane protein of Edwardsiella tarda.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Edwardsiella tarda is an important cause for hemorrhagic septicemia in fish and gastro and extra-intestinal infections in humans. Monoclonal antibodies (MAbs) were produced against outer membrane proteins (OMPs) of E. tarda ET-7, isolated from diseased snakehead (Ophiocephalus punctatus). Two stable hybridoma clones, designated as 3F10 and 2C3 MAbs were found to be potentially specific for E. tarda by indirect enzyme linked immunosorbent assay (ELISA). These MAbs recognized major immunogenic OMP band at 44kDa in Western blotting. Both MAbs belonged to the IgG1 isotype and recognized different epitopes of OMP as seen by...
Source: Comparative immunology, microbiology and infectious diseases. - September 18, 2008 Category: Allergy & Immunology Authors: Kumar G, Rathore G, Sengupta U, Kapoor D, Lakra WS Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Innate immune activation of swine intestinal epithelial cell lines (IPEC-J2 and IPI-2I) in response to LPS from Salmonella typhimurium.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the porcine intestinal innate immune response was evaluated by analysing the expression of TLRs, cytokines and chemokines in two porcine epithelial cell lines from different regions: IPEC-J2 (jejunum) and IPI-2I (ileum). Both cells lines were stimulated with 1mug of LPS from Salmonella typhimurium. RNA was collected at 30min, 1, 2, 3 and 4h after treatment. Expression of TLR-1, -2, -3, -4, -6, -8, -9, -10, TNF-alpha, IL-1beta, -8 and MCP-1 was quantified relative to the quantity of Cyclophilin-A mRNA using real-time quantitative PCR (RTQ-PCR). The results obtained show up differences in the gene expression b...
Source: Comparative immunology, microbiology and infectious diseases. - September 15, 2008 Category: Allergy & Immunology Authors: Arce C, Ramírez-Boo M, Lucena C, Garrido JJ Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Molecular cloning, expression and first antigenic characterization of human astrovirus VP26 structural protein and a C-terminal deleted form.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we describe for the first time the cloning and expression in Escherichia coli (E. coli) of a recombinant VP26 (rVP26) protein and a VP26 C-terminal truncated form (VP26DeltaC), followed by purification by NTA-Ni(2+) agarose affinity chromatography. Protein expression and purification were evaluated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot (WB). Then, the purified proteins were evaluated for antigenic properties in enzyme linked immunosorbent assay (ELISA) using a polyclonal antibody (PAb) and a neutralizing monoclonal antibody (nMAb) named PL2, both of them di...
Source: Comparative immunology, microbiology and infectious diseases. - September 12, 2008 Category: Allergy & Immunology Authors: Royuela E, Sánchez-Fauquier A Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Epizootology and experimental infection of Yokose virus in bats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To reveal whether bats serve as an amplifying host for Yokose virus (YOKV), we conducted a serological survey and experimentally infected fruit bats with YOKV isolated from microbats in Japan. YOKV belongs to the Entebbe bat virus group of vector unknown group within the genus Flavivirus and family Flaviviridae. To detect antibodies against YOKV, we developed an enzyme-linked immunosorbent assay (ELISA) using biotinylated anti-bat IgG rabbit sera. Serological surveillance was conducted with samples collected in the Philippines and the sera supplied from Malaysia. One of the 36 samples from the Philippines (2.7%) and 5 ...
Source: Comparative immunology, microbiology and infectious diseases. - September 10, 2008 Category: Allergy & Immunology Authors: Watanabe S, Omatsu T, Miranda ME, Masangkay JS, Ueda N, Endo M, Kato K, Tohya Y, Yoshikawa Y, Akashi H Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Origin of Clostridium perfringens isolates determines the ability to induce necrotic enteritis in broilers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Since the ban on growth-promoting antibiotics in animal feed in the European Union, necrotic enteritis has become a major cause of mortality in broiler chickens. Despite the importance of the disease, the pathogenesis is still not completely understood. In the current study, Clostridium perfringens strains isolated from healthy flocks and isolates from outbreaks of necrotic enteritis were evaluated for the ability to cause gut necrosis in an intestinal loop model in laying hens and in an experimental infection model in broilers. High, intermediate and low alpha toxin producing strains were chosen from each isolation so...
Source: Comparative immunology, microbiology and infectious diseases. - September 8, 2008 Category: Allergy & Immunology Authors: Timbermont L, Lanckriet A, Gholamiandehkordi AR, Pasmans F, Martel A, Haesebrouck F, Ducatelle R, Van Immerseel F Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Pathological and phylogenetic features of prevalent canine distemper viruses in wild masked palm civets in Japan.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ten wild masked palm civets infected with canine distemper virus (CDV), captured in Japan from 2005 to 2007, were histopathologically and phylogenetically analyzed. Phylogenetic analysis based on the amino acid sequences of the H protein of two CDV isolates from masked palm civets revealed that the two isolates were classified into the clade of recent isolates in Japan. Histopathologically marked lesions of virus encephalitis were present in the brain, whereas gastrointestinal lesions were absent or at a mild degree. The distribution of the lesions resembles that of recent CDV cases in dogs. Therefore, recent CDV infec...
Source: Comparative immunology, microbiology and infectious diseases. - September 5, 2008 Category: Allergy & Immunology Authors: Takayama I, Kubo M, Takenaka A, Fujita K, Sugiyama T, Arai T, Yoneda M, Sato H, Yanai T, Kai C Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Intraspecific variation in 16S rRNA gene of Mycoplasma synoviae determined by DNA sequencing.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mycoplasma synoviae (MS) is an important avian pathogen may cause both respiratory disease and joint inflammation synovitis in poultry, causing economic losses to the Brazilian poultry industry. The genotypic variation in 16S rRNA gene is unknown. Partial sequences of 16S rRNA gene of 19 strains of M. synoviae were sequenced and analyzed in order to obtain molecular characterization and evaluation of the genetic variability of strains from distinct Brazilian areas of poultry production. Different polymorphic patterns were observed. The number of polymorphic alterations in the studied strains ranged from 0 to 6. The nuc...
Source: Comparative immunology, microbiology and infectious diseases. - September 3, 2008 Category: Allergy & Immunology Authors: Buim MR, Buzinhani M, Yamaguti M, Oliveira RC, Mettifogo E, Timenetsky J, Ferreira AJ Tags: Comp Immunol Microbiol Infect Dis Source Type: journals

Proteomic profiling of ovine serum by SELDI-TOF MS: Optimisation, reproducibility and feasibility of biomarker discovery using routinely collected samples.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The diagnosis of infectious diseases in animals may be enhanced by study of the serum proteome in which myriad components are influenced by physiological and pathological processes. Surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-TOF MS) has the capacity to detect known and unknown immunologically relevant molecules in the serum proteome. Optimum combinations of ProteinChip((R)) array surfaces, energy absorbing molecules, sample dilutions and instrument settings were determined for spectral generation from whole ovine sera. The coefficient of variation for within and between chip ma...
Source: Comparative immunology, microbiology and infectious diseases. - September 3, 2008 Category: Allergy & Immunology Authors: Zhong L, Taylor DL, Whittington RJ Tags: Comp Immunol Microbiol Infect Dis Source Type: journals