Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research

Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research

Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research

Computer-aided exploration of multiobjective optimal temperature profiles in slow freezing for human induced pluripotent stem cells
This study was based on a model that calculates cell survival rates after thawing, and the model was extended to evaluate cell potentials until 24 h after seeding. To estimate parameter values for this extension, freezing experiments were performed using constant cooling rates. Using quality and productivity indicators, we evaluated 16,206 temperature profiles using our model, and a promising profile was obtained. Finally, an experimental investigation of the profile was undertaken, and the contribution of the temperature profile to both quality and productivity was confirmed.PMID:38513997 | DOI:10.1016/j.cryobiol.2024.104...
Source: Cryobiology - March 21, 2024 Category: Biology Authors: Yusuke Hayashi Yuki Uno Masahiro Kino-Oka Hirokazu Sugiyama Source Type: research

Cryopreserved leukapheresis material can Be transferred from controlled rate freezers to ultracold storage at warmer temperatures without affecting downstream CAR-T cell culture performance and in-vitro functionality
This study aims to determine the optimal temperature range for transferring cryopreserved leukapheresis materials from two distinct types of controlled rate freezing systems, Liquid Nitrogen (LN2)-based and LN2-free Conduction Cooling-based, to the ultracold LN2 storage freezer (≤-135 °C), and its impact on CAR T-cell production and functionality. Presented findings demonstrate that there is no significant influence on CAR T-cell expansion, differentiation, or downstream in-vitro function when employing a transfer temperature range spanning from -30 °C to -80 °C for the LN2-based controlled rate freezers as well as fo...
Source: Cryobiology - March 21, 2024 Category: Biology Authors: Jiaming Wei Katherine Chaney Woo Jin Shim Heyu Chen Grace Leonard Sean O'Brien Ziyan Liu Jinlin Jiang Robert Ulrey Source Type: research

Computer-aided exploration of multiobjective optimal temperature profiles in slow freezing for human induced pluripotent stem cells
This study was based on a model that calculates cell survival rates after thawing, and the model was extended to evaluate cell potentials until 24 h after seeding. To estimate parameter values for this extension, freezing experiments were performed using constant cooling rates. Using quality and productivity indicators, we evaluated 16,206 temperature profiles using our model, and a promising profile was obtained. Finally, an experimental investigation of the profile was undertaken, and the contribution of the temperature profile to both quality and productivity was confirmed.PMID:38513997 | DOI:10.1016/j.cryobiol.2024.104...
Source: Cryobiology - March 21, 2024 Category: Biology Authors: Yusuke Hayashi Yuki Uno Masahiro Kino-Oka Hirokazu Sugiyama Source Type: research

Cryopreserved leukapheresis material can Be transferred from controlled rate freezers to ultracold storage at warmer temperatures without affecting downstream CAR-T cell culture performance and in-vitro functionality
This study aims to determine the optimal temperature range for transferring cryopreserved leukapheresis materials from two distinct types of controlled rate freezing systems, Liquid Nitrogen (LN2)-based and LN2-free Conduction Cooling-based, to the ultracold LN2 storage freezer (≤-135 °C), and its impact on CAR T-cell production and functionality. Presented findings demonstrate that there is no significant influence on CAR T-cell expansion, differentiation, or downstream in-vitro function when employing a transfer temperature range spanning from -30 °C to -80 °C for the LN2-based controlled rate freezers as well as fo...
Source: Cryobiology - March 21, 2024 Category: Biology Authors: Jiaming Wei Katherine Chaney Woo Jin Shim Heyu Chen Grace Leonard Sean O'Brien Ziyan Liu Jinlin Jiang Robert Ulrey Source Type: research

Computer-aided exploration of multiobjective optimal temperature profiles in slow freezing for human induced pluripotent stem cells
This study was based on a model that calculates cell survival rates after thawing, and the model was extended to evaluate cell potentials until 24 h after seeding. To estimate parameter values for this extension, freezing experiments were performed using constant cooling rates. Using quality and productivity indicators, we evaluated 16,206 temperature profiles using our model, and a promising profile was obtained. Finally, an experimental investigation of the profile was undertaken, and the contribution of the temperature profile to both quality and productivity was confirmed.PMID:38513997 | DOI:10.1016/j.cryobiol.2024.104...
Source: Cryobiology - March 21, 2024 Category: Biology Authors: Yusuke Hayashi Yuki Uno Masahiro Kino-Oka Hirokazu Sugiyama Source Type: research

Cryopreserved leukapheresis material can Be transferred from controlled rate freezers to ultracold storage at warmer temperatures without affecting downstream CAR-T cell culture performance and in-vitro functionality
This study aims to determine the optimal temperature range for transferring cryopreserved leukapheresis materials from two distinct types of controlled rate freezing systems, Liquid Nitrogen (LN2)-based and LN2-free Conduction Cooling-based, to the ultracold LN2 storage freezer (≤-135 °C), and its impact on CAR T-cell production and functionality. Presented findings demonstrate that there is no significant influence on CAR T-cell expansion, differentiation, or downstream in-vitro function when employing a transfer temperature range spanning from -30 °C to -80 °C for the LN2-based controlled rate freezers as well as fo...
Source: Cryobiology - March 21, 2024 Category: Biology Authors: Jiaming Wei Katherine Chaney Woo Jin Shim Heyu Chen Grace Leonard Sean O'Brien Ziyan Liu Jinlin Jiang Robert Ulrey Source Type: research

Sperm characteristics of cryopreserved Prochilodus lineatus semen after adding cholesterol-loaded cyclodextrin
Cryobiology. 2024 Mar 18:104888. doi: 10.1016/j.cryobiol.2024.104888. Online ahead of print.ABSTRACTThe experiment evaluated the effect of adding cholesterol-loaded cyclodextrin (CLC) to Prochilodus lineatus fish (Curimata) semen on post-thaw sperm quality. Twelve adult fish were used for sperm collection after induced spermiation with carp pituitary gland. The semen was diluted and treated with CLC in concentrations of 0 (control), 0.5, 1.0, 2.0, 3.0, and 4.0 mg for 120 × 106 spermatozoa/ml, loaded in 0.5 ml straws, packaged and placed in dry vapor vessel cylinders for 24 h before being submerged in liquid nitrogen for s...
Source: Cryobiology - March 20, 2024 Category: Biology Authors: K V Antunes J J S Santos I C S Carvalho E M S Moreira G R Moreira L D S Murgas E A Moraes Source Type: research

Sperm characteristics of cryopreserved Prochilodus lineatus semen after adding cholesterol-loaded cyclodextrin
Cryobiology. 2024 Mar 18:104888. doi: 10.1016/j.cryobiol.2024.104888. Online ahead of print.ABSTRACTThe experiment evaluated the effect of adding cholesterol-loaded cyclodextrin (CLC) to Prochilodus lineatus fish (Curimata) semen on post-thaw sperm quality. Twelve adult fish were used for sperm collection after induced spermiation with carp pituitary gland. The semen was diluted and treated with CLC in concentrations of 0 (control), 0.5, 1.0, 2.0, 3.0, and 4.0 mg for 120 × 106 spermatozoa/ml, loaded in 0.5 ml straws, packaged and placed in dry vapor vessel cylinders for 24 h before being submerged in liquid nitrogen for s...
Source: Cryobiology - March 20, 2024 Category: Biology Authors: K V Antunes J J S Santos I C S Carvalho E M S Moreira G R Moreira L D S Murgas E A Moraes Source Type: research

A droplet vitrification cryopreservation protocol for conservation of hops (Humulus lupulus) genetic resources
Cryobiology. 2024 Mar 15:104887. doi: 10.1016/j.cryobiol.2024.104887. Online ahead of print.ABSTRACTHops (Humulus lupulus L.) is essentially used in the brewing industry as it contributes to flavor, and aroma of beer. However, the genetic diversity of hops is increasingly threatened by diseases, environmental changes, and urbanization. Cryopreservation has emerged as a pivotal strategy for safeguarding and maintaining the genetic diversity of hops. The present work presents a comprehensive study on the cryopreservation of hops, focusing on the development and optimization of a droplet vitrification based cryopreservation p...
Source: Cryobiology - March 17, 2024 Category: Biology Authors: Era Vaidya Malhotra Suresh Chand Mali Shreya Sharma Sangita Bansal Source Type: research

A droplet vitrification cryopreservation protocol for conservation of hops (Humulus lupulus) genetic resources
Cryobiology. 2024 Mar 15:104887. doi: 10.1016/j.cryobiol.2024.104887. Online ahead of print.ABSTRACTHops (Humulus lupulus L.) is essentially used in the brewing industry as it contributes to flavor, and aroma of beer. However, the genetic diversity of hops is increasingly threatened by diseases, environmental changes, and urbanization. Cryopreservation has emerged as a pivotal strategy for safeguarding and maintaining the genetic diversity of hops. The present work presents a comprehensive study on the cryopreservation of hops, focusing on the development and optimization of a droplet vitrification based cryopreservation p...
Source: Cryobiology - March 17, 2024 Category: Biology Authors: Era Vaidya Malhotra Suresh Chand Mali Shreya Sharma Sangita Bansal Source Type: research

A droplet vitrification cryopreservation protocol for conservation of hops (Humulus lupulus) genetic resources
Cryobiology. 2024 Mar 15:104887. doi: 10.1016/j.cryobiol.2024.104887. Online ahead of print.ABSTRACTHops (Humulus lupulus L.) is essentially used in the brewing industry as it contributes to flavor, and aroma of beer. However, the genetic diversity of hops is increasingly threatened by diseases, environmental changes, and urbanization. Cryopreservation has emerged as a pivotal strategy for safeguarding and maintaining the genetic diversity of hops. The present work presents a comprehensive study on the cryopreservation of hops, focusing on the development and optimization of a droplet vitrification based cryopreservation p...
Source: Cryobiology - March 17, 2024 Category: Biology Authors: Era Vaidya Malhotra Suresh Chand Mali Shreya Sharma Sangita Bansal Source Type: research

l-carnitine enhances the kinematics and protects the sperm membranes of chilled and frozen-thawed Peruvian Paso horse spermatozoa
In conclusion, supplementation of l-carnitine to skimmed milk-based extenders enhanced kinematic variables and protected the membrane integrity in chilled and frozen-thawed Peruvian Paso horse spermatozoa.PMID:38460835 | DOI:10.1016/j.cryobiol.2024.104884 (Source: Cryobiology)
Source: Cryobiology - March 9, 2024 Category: Biology Authors: Paula Palacios Gabriela Pel áez Manuel Soria Silvana M éndez Luis Galarza- Álvarez Jes ús Dorado Juli án Santiago-Moreno Diego A Galarza Source Type: research