DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice
Dev Cell. 2024 Apr 22:S1534-5807(24)00227-2. doi: 10.1016/j.devcel.2024.04.002. Online ahead of print.ABSTRACTRNA-binding proteins (RBPs), as key regulators of mRNA fate, are abundantly expressed in the testis. However, RBPs associated with human male infertility remain largely unknown. Through bioinformatic analyses, we identified 62 such RBPs, including an evolutionarily conserved RBP, DEAD-box helicase 20 (DDX20). Male germ-cell-specific inactivation of Ddx20 at E15.5 caused T1-propsermatogonia (T1-ProSG) to fail to reenter cell cycle during the first week of testicular development in mice. Consequently, neither the fou...
Source: Developmental Cell - April 24, 2024 Category: Cytology Authors: Dingfeng Zou Kai Li Luying Su Jun Liu Yan Lu Rong Huang Mengzhen Li Xinyu Mang Qi Geng Pengyu Li Jielin Tang Zhixin Yu Zexuan Zhang Dingyao Chen Shiying Miao Jia Yu Wei Yan Wei Song Source Type: research
Unlocking trophectoderm mysteries: In vivo and in vitro perspectives on human and mouse trophoblast fate induction
Dev Cell. 2024 Apr 22;59(8):941-960. doi: 10.1016/j.devcel.2024.03.029.ABSTRACTIn recent years, the pursuit of inducing the trophoblast stem cell (TSC) state has gained prominence as a compelling research objective, illuminating the establishment of the trophoblast lineage and unlocking insights into early embryogenesis. In this review, we examine how advancements in diverse technologies, including in vivo time course transcriptomics, cellular reprogramming to TSC state, chemical induction of totipotent stem-cell-like state, and stem-cell-based embryo-like structures, have enriched our insights into the intricate molecular...
Source: Developmental Cell - April 23, 2024 Category: Cytology Authors: Meir Azagury Yosef Buganim Source Type: research
Antagonistic control of rice immunity against distinct pathogens by the two transcription modules via salicylic acid and jasmonic acid pathways
Dev Cell. 2024 Apr 10:S1534-5807(24)00201-6. doi: 10.1016/j.devcel.2024.03.033. Online ahead of print.ABSTRACTAlthough the antagonistic effects of host resistance against biotrophic and necrotrophic pathogens have been documented in various plants, the underlying mechanisms are unknown. Here, we investigated the antagonistic resistance mediated by the transcription factor ETHYLENE-INSENSITIVE3-LIKE 3 (OsEIL3) in rice. The Oseil3 mutant confers enhanced resistance to the necrotroph Rhizoctonia solani but greater susceptibility to the hemibiotroph Magnaporthe oryzae and biotroph Xanthomonas oryzae pv. oryzae. OsEIL3 directly...
Source: Developmental Cell - April 19, 2024 Category: Cytology Authors: Xiaoying Zhu Yudan Zhao Cheng-Min Shi Guojuan Xu Nana Wang Shimin Zuo Yuese Ning Houxiang Kang Wende Liu Ruyi Wang Shuangyong Yan Guo-Liang Wang Xuli Wang Source Type: research
Pten, PI3K, and PtdIns(3,4,5)P < sub > 3 < /sub > dynamics control pulsatile actin branching in Drosophila retina morphogenesis
Dev Cell. 2024 Apr 17:S1534-5807(24)00203-X. doi: 10.1016/j.devcel.2024.03.035. Online ahead of print.ABSTRACTEpithelial remodeling of the Drosophila retina depends on the pulsatile contraction and expansion of apical contacts between the cells that form its hexagonal lattice. Phosphoinositide PI(3,4,5)P3 (PIP3) accumulates around tricellular adherens junctions (tAJs) during contact expansion and dissipates during contraction, but with unknown function. Here, we found that manipulations of Pten or PI3-kinase (PI3K) that either decreased or increased PIP3 resulted in shortened contacts and a disordered lattice, indicating a...
Source: Developmental Cell - April 19, 2024 Category: Cytology Authors: Jacob Malin Christian Rosa-Birriel Victor Hatini Source Type: research
Sustained ERK signaling promotes G2 cell cycle exit and primes cells for whole-genome duplication
Dev Cell. 2024 Apr 11:S1534-5807(24)00200-4. doi: 10.1016/j.devcel.2024.03.032. Online ahead of print.ABSTRACTWhole-genome duplication (WGD) is a frequent event in cancer evolution that fuels chromosomal instability. WGD can result from mitotic errors or endoreduplication, yet the molecular mechanisms that drive WGD remain unclear. Here, we use live single-cell analysis to characterize cell-cycle dynamics upon aberrant Ras-ERK signaling. We find that sustained ERK signaling in human cells leads to reactivation of the APC/C in G2, resulting in tetraploid G0-like cells that are primed for WGD. This process is independent of ...
Source: Developmental Cell - April 19, 2024 Category: Cytology Authors: Adler Guerrero Zuniga Timothy J Aikin Connor McKenney Yovel Lendner Alain Phung Paul W Hook Amy Meltzer Winston Timp Sergi Regot Source Type: research
Antagonistic control of rice immunity against distinct pathogens by the two transcription modules via salicylic acid and jasmonic acid pathways
Dev Cell. 2024 Apr 10:S1534-5807(24)00201-6. doi: 10.1016/j.devcel.2024.03.033. Online ahead of print.ABSTRACTAlthough the antagonistic effects of host resistance against biotrophic and necrotrophic pathogens have been documented in various plants, the underlying mechanisms are unknown. Here, we investigated the antagonistic resistance mediated by the transcription factor ETHYLENE-INSENSITIVE3-LIKE 3 (OsEIL3) in rice. The Oseil3 mutant confers enhanced resistance to the necrotroph Rhizoctonia solani but greater susceptibility to the hemibiotroph Magnaporthe oryzae and biotroph Xanthomonas oryzae pv. oryzae. OsEIL3 directly...
Source: Developmental Cell - April 19, 2024 Category: Cytology Authors: Xiaoying Zhu Yudan Zhao Cheng-Min Shi Guojuan Xu Nana Wang Shimin Zuo Yuese Ning Houxiang Kang Wende Liu Ruyi Wang Shuangyong Yan Guo-Liang Wang Xuli Wang Source Type: research
Pten, PI3K, and PtdIns(3,4,5)P < sub > 3 < /sub > dynamics control pulsatile actin branching in Drosophila retina morphogenesis
Dev Cell. 2024 Apr 17:S1534-5807(24)00203-X. doi: 10.1016/j.devcel.2024.03.035. Online ahead of print.ABSTRACTEpithelial remodeling of the Drosophila retina depends on the pulsatile contraction and expansion of apical contacts between the cells that form its hexagonal lattice. Phosphoinositide PI(3,4,5)P3 (PIP3) accumulates around tricellular adherens junctions (tAJs) during contact expansion and dissipates during contraction, but with unknown function. Here, we found that manipulations of Pten or PI3-kinase (PI3K) that either decreased or increased PIP3 resulted in shortened contacts and a disordered lattice, indicating a...
Source: Developmental Cell - April 19, 2024 Category: Cytology Authors: Jacob Malin Christian Rosa-Birriel Victor Hatini Source Type: research
Sustained ERK signaling promotes G2 cell cycle exit and primes cells for whole-genome duplication
Dev Cell. 2024 Apr 11:S1534-5807(24)00200-4. doi: 10.1016/j.devcel.2024.03.032. Online ahead of print.ABSTRACTWhole-genome duplication (WGD) is a frequent event in cancer evolution that fuels chromosomal instability. WGD can result from mitotic errors or endoreduplication, yet the molecular mechanisms that drive WGD remain unclear. Here, we use live single-cell analysis to characterize cell-cycle dynamics upon aberrant Ras-ERK signaling. We find that sustained ERK signaling in human cells leads to reactivation of the APC/C in G2, resulting in tetraploid G0-like cells that are primed for WGD. This process is independent of ...
Source: Developmental Cell - April 19, 2024 Category: Cytology Authors: Adler Guerrero Zuniga Timothy J Aikin Connor McKenney Yovel Lendner Alain Phung Paul W Hook Amy Meltzer Winston Timp Sergi Regot Source Type: research
Mannose controls mesoderm specification and symmetry breaking in mouse gastruloids
Dev Cell. 2024 Apr 9:S1534-5807(24)00198-9. doi: 10.1016/j.devcel.2024.03.031. Online ahead of print.ABSTRACTPatterning and growth are fundamental features of embryonic development that must be tightly coordinated. To understand how metabolism impacts early mesoderm development, we used mouse embryonic stem-cell-derived gastruloids, that co-expressed glucose transporters with the mesodermal marker T/Bra. We found that the glucose mimic, 2-deoxy-D-glucose (2-DG), blocked T/Bra expression and abolished axial elongation in gastruloids. However, glucose removal did not phenocopy 2-DG treatment despite a decline in glycolytic i...
Source: Developmental Cell - April 18, 2024 Category: Cytology Authors: Chaitanya Dingare Dominica Cao Jenny Jingni Yang Berna Sozen Benjamin Steventon Source Type: research
Identification of signaling pathways that specify a subset of migrating enteric neural crest cells at the wavefront in mouse embryos
Dev Cell. 2024 Apr 15:S1534-5807(24)00202-8. doi: 10.1016/j.devcel.2024.03.034. Online ahead of print.ABSTRACTDuring enteric nervous system (ENS) development, pioneering wavefront enteric neural crest cells (ENCCs) initiate gut colonization. However, the molecular mechanisms guiding their specification and niche interaction are not fully understood. We used single-cell RNA sequencing and spatial transcriptomics to map the spatiotemporal dynamics and molecular landscape of wavefront ENCCs in mouse embryos. Our analysis shows a progressive decline in wavefront ENCC potency during migration and identifies transcription factor...
Source: Developmental Cell - April 18, 2024 Category: Cytology Authors: Bingyan Zhou Chenzhao Feng Song Sun Xuyong Chen Didi Zhuansun Di Wang Xiaosi Yu Xinyao Meng Jun Xiao Luyao Wu Jing Wang Jing Wang Ke Chen Zejian Li Jingyi You Handan Mao Shimin Yang Jiaxin Zhang Chunlei Jiao Zhi Li Donghai Yu Xiaojuan Wu Tianqi Zhu Jixin Source Type: research
Mannose controls mesoderm specification and symmetry breaking in mouse gastruloids
Dev Cell. 2024 Apr 9:S1534-5807(24)00198-9. doi: 10.1016/j.devcel.2024.03.031. Online ahead of print.ABSTRACTPatterning and growth are fundamental features of embryonic development that must be tightly coordinated. To understand how metabolism impacts early mesoderm development, we used mouse embryonic stem-cell-derived gastruloids, that co-expressed glucose transporters with the mesodermal marker T/Bra. We found that the glucose mimic, 2-deoxy-D-glucose (2-DG), blocked T/Bra expression and abolished axial elongation in gastruloids. However, glucose removal did not phenocopy 2-DG treatment despite a decline in glycolytic i...
Source: Developmental Cell - April 18, 2024 Category: Cytology Authors: Chaitanya Dingare Dominica Cao Jenny Jingni Yang Berna Sozen Benjamin Steventon Source Type: research
Identification of signaling pathways that specify a subset of migrating enteric neural crest cells at the wavefront in mouse embryos
Dev Cell. 2024 Apr 15:S1534-5807(24)00202-8. doi: 10.1016/j.devcel.2024.03.034. Online ahead of print.ABSTRACTDuring enteric nervous system (ENS) development, pioneering wavefront enteric neural crest cells (ENCCs) initiate gut colonization. However, the molecular mechanisms guiding their specification and niche interaction are not fully understood. We used single-cell RNA sequencing and spatial transcriptomics to map the spatiotemporal dynamics and molecular landscape of wavefront ENCCs in mouse embryos. Our analysis shows a progressive decline in wavefront ENCC potency during migration and identifies transcription factor...
Source: Developmental Cell - April 18, 2024 Category: Cytology Authors: Bingyan Zhou Chenzhao Feng Song Sun Xuyong Chen Didi Zhuansun Di Wang Xiaosi Yu Xinyao Meng Jun Xiao Luyao Wu Jing Wang Jing Wang Ke Chen Zejian Li Jingyi You Handan Mao Shimin Yang Jiaxin Zhang Chunlei Jiao Zhi Li Donghai Yu Xiaojuan Wu Tianqi Zhu Jixin Source Type: research
Accumulation of APP C-terminal fragments causes endolysosomal dysfunction through the dysregulation of late endosome to lysosome-ER contact sites
Dev Cell. 2024 Apr 12:S1534-5807(24)00199-0. doi: 10.1016/j.devcel.2024.03.030. Online ahead of print.ABSTRACTNeuronal endosomal and lysosomal abnormalities are among the early changes observed in Alzheimer's disease (AD) before plaques appear. However, it is unclear whether distinct endolysosomal defects are temporally organized and how altered γ-secretase function or amyloid precursor protein (APP) metabolism contribute to these changes. Inhibiting γ-secretase chronically, in mouse embryonic fibroblast and hippocampal neurons, led to a gradual endolysosomal collapse initiated by decreased lysosomal calcium and increase...
Source: Developmental Cell - April 16, 2024 Category: Cytology Authors: Marine Bretou Ragna Sannerud Abril Escamilla-Ayala Tom Leroy C éline Vrancx Zo ë P Van Acker Anika Perdok Wendy Vermeire Inge Vorsters Sophie Van Keymolen Michelle Maxson Benjamin Pavie Keimpe Wierda Eeva-Liisa Eskelinen Wim Annaert Source Type: research
Accumulation of APP C-terminal fragments causes endolysosomal dysfunction through the dysregulation of late endosome to lysosome-ER contact sites
Dev Cell. 2024 Apr 12:S1534-5807(24)00199-0. doi: 10.1016/j.devcel.2024.03.030. Online ahead of print.ABSTRACTNeuronal endosomal and lysosomal abnormalities are among the early changes observed in Alzheimer's disease (AD) before plaques appear. However, it is unclear whether distinct endolysosomal defects are temporally organized and how altered γ-secretase function or amyloid precursor protein (APP) metabolism contribute to these changes. Inhibiting γ-secretase chronically, in mouse embryonic fibroblast and hippocampal neurons, led to a gradual endolysosomal collapse initiated by decreased lysosomal calcium and increase...
Source: Developmental Cell - April 16, 2024 Category: Cytology Authors: Marine Bretou Ragna Sannerud Abril Escamilla-Ayala Tom Leroy C éline Vrancx Zo ë P Van Acker Anika Perdok Wendy Vermeire Inge Vorsters Sophie Van Keymolen Michelle Maxson Benjamin Pavie Keimpe Wierda Eeva-Liisa Eskelinen Wim Annaert Source Type: research
No need to "sugar coat": Removing glycocalyx on apoptotic blebs promotes phagocytosis
Dev Cell. 2024 Apr 8;59(7):827-829. doi: 10.1016/j.devcel.2024.02.014.ABSTRACTThe viscous glycocalyx of mammalian cells, composed of glucosaminoglycans, glycolipids, and glycoproteins, "sugar coat" the outer plasma membrane. In this issue of Developmental Cell, Le et al. (2024) show that the glycocalyx is removed from apoptotic blebs via disassembly of the cortical cytoskeleton, exposing the "eat-me" signals necessary for efferocytosis.PMID:38593785 | DOI:10.1016/j.devcel.2024.02.014 (Source: Developmental Cell)
Source: Developmental Cell - April 9, 2024 Category: Cytology Authors: Shannon M Kelley Kodi S Ravichandran Source Type: research
