European Journal of Clinical Microbiology & Infectious DiseasesThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

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Hantavirus nephropathy as a pseudo-import pathology from EcuadorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a case of hantavirus infection (nephropathia epidemica) diagnosed in a Belgian backpacker returning from a trekking expedition in Ecuador, after likely heavy exposure to rodents. Because of epidemiological inconsistency, molecular investigation was performed and revealed a Puumala infection acquired during very limited exposure in Belgium upon return. Content Type Journal ArticleCategory ArticleDOI 10.1007/s10096-009-0820-7Authors R. Demeester, Department of Internal Medicine University Hospital Saint-Pierre Rue Haute, 322 1000 Brussels BelgiumE. Bottieau, Institute of Tropical Medicine Department of Clini...
Source: European Journal of Clinical Microbiology & Infectious Diseases - October 11, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

The intracellular effects of manuka honey on Staphylococcus aureusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The purpose of this study was to investigate the effect of manuka honey on Staphylococcus aureus in order to identify the intracellular target site. The mode of inhibition of manuka honey against S. aureus NCTC 10017 was investigated by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and the effect of time on viability. Structural changes were observed by scanning (SEM) and transmission electron microscopy (TEM) of cells suspended for 4 h at 37°C in 0.05 mM Tris buffer containing 10% (w/v) manuka honey and were compared to cells in buffer alone o...
Source: European Journal of Clinical Microbiology & Infectious Diseases - October 8, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Molecular characteristics of pharyngeal and invasive emm3 Streptococcus pyogenes strains from Norway, 1988–2003Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract A major virulence factor of group A streptococci (GAS) is the M protein. Strains with the M3 type are more often associated with necrotizing fasciitis (NF) and streptococcal toxic shock syndrome, and have a higher case fatality rate than strains of other M types. To better understand the epidemiology of M3 GAS strains in Norway, we analyzed 59 invasive and 69 pharyngeal isolates with respect to prophage content, allelic variation in emm3, mtsR encoding the metal transporter of Streptococcus repressor (mtsR), and sclB coding for streptococcal collagen-like protein B. The Norwegian emm3 strains were ve...
Source: European Journal of Clinical Microbiology & Infectious Diseases - October 6, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Metabolic properties of lactobacilli in women experiencing recurring episodes of bacterial vaginosis with vaginal pH ≥ 5Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract While 60% of women experiencing recurring episodes of bacterial vaginosis (BV) with vaginal pH ≥ 5 are depleted of resident probiotic lactobacilli, the remainder carry one or more strains of lactobacilli. Their ability to make D-lactic acid is, however, low (3.94 ± 0.72 mM/L) compared to the D-lactic acid produced by strains from healthy vagina with vaginal pH ∼ 4 (8.04 ± 1.07 mM/L) culture supernatant of 0.5 McFarland concentration (P < 0.001). Content Type Journal ArticleCategory Brief ReportDOI 10.1007/s10096-009-0818-1Authors K. B. Garg, The Talwar Rese...
Source: European Journal of Clinical Microbiology & Infectious Diseases - October 5, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity. Content Type Journal ArticleCategory Brief ReportDOI 10.1007/s10096-009-0816-3Authors W. J. B. van Wamel, University Medical Centre Rotterdam ErasmusMC Rotterdam The NetherlandsS. Hansenová Maňásková, University Medical Cen...
Source: European Journal of Clinical Microbiology & Infectious Diseases - October 1, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

First report on methicillin-resistant Staphylococcus aureus of Spa type T037, Sequence type 239, SCCmec type III/IIIA in MalaysiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) from Malaysia were shown to possess staphylococcal cassette chromosome mec (SCCmec)-III and IIIA. Spa sequencing and multi-locus sequence typing (MLST) documented t037 and ST 239 (CC8) for 83.3% of the isolates. This confirms observations in several other Far Eastern countries and corroborates the epidemicity of this clone. Content Type Journal ArticleCategory Brief ReportDOI 10.1007/s10096-009-0813-6Authors V. Neela, Universiti Putra Malaysia Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences Serdang 4340...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 24, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Expression, purification, and characterization of the humoral immune response to recombinant MyfA protein of Yersinia enterocoliticaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Content Type Journal ArticleCategory Brief ReportDOI 10.1007/s10096-009-0812-7Authors W. Rastawicki, National Institute of Public Health— National Institute of Hygiene Department of Bacteriology Chocimska 24 00-791 Warsaw PolandR. Gierczyński, National Institute of Public Health— National Institute of Hygiene Department of Bacteriology Chocimska 24 00-791 Warsaw Poland Journal European Journal of Clinical Microbiology & Infectious DiseasesOnline ISSN 1435-4373Print ISSN 0934-9723
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 20, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Fosfomycin in a single dose versus a 7-day course of amoxicillin–clavulanate for the treatment of asymptomatic bacteriuria during pregnancyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The purpose of this paper was to compare the efficacy of a single dose of 3 g of fosfomycin to that of a 7-day regimen of amoxicillin–clavulanate in the treatment of asymptomatic bacteriuria during pregnancy. A randomised, prospective, interventional, analytical, longitudinal study was undertaken, in which the efficacy of two antibiotic regimens (one short and the other long) in the treatment of pregnant women with asymptomatic bacteriuria is compared. One hundred and nine patients were randomly assigned to two groups: 56 were treated with amoxicillin–clavulanate and 53 with fosfomycin. The...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 20, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Erratum to: Effect of meteorological variables on the incidence of lower urinary tract infectionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Content Type Journal ArticleCategory ErratumDOI 10.1007/s10096-009-0815-4Authors M. E. Falagas, Alfa Institute of Biomedical Sciences (AIBS) 9 Neapoleos Street, 151 23 Marousi Athens GreeceG. Peppas, Alfa Institute of Biomedical Sciences (AIBS) 9 Neapoleos Street, 151 23 Marousi Athens GreeceD. K. Matthaiou, Alfa Institute of Biomedical Sciences (AIBS) 9 Neapoleos Street, 151 23 Marousi Athens GreeceD. E. Karageorgopoulos, Alfa Institute of Biomedical Sciences (AIBS) 9 Neapoleos Street, 151 23 Marousi Athens GreeceN. Karalis, National Technical University of Athens Athens GreeceG. Theocharis, SOS Doctors Athens Greece ...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 18, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Genetic relatedness and virulence gene profiles of Escherichia coli strains isolated from septicaemic and uroseptic patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract We investigated the relationship between clonality and virulence factors (VFs) of a collection of Escherichia coli strains isolated from septicaemic and uroseptic patients with respect to their origin of translocation. Forty septicaemic and 30 uroseptic strains of E. coli were tested for their phylogenetic groupings, genetic relatedness using randomly amplified polymorphic DNA (RAPD), biochemical fingerprinting method (biochemical phenotypes [BPTs]), adherence to HT-29 cells and the presence of 56 E. coli VF genes. Strains belonging to phylogenetic groups B2 and D constituted 93% of all strains. Fifty...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 18, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Oral valganciclovir treatment in newborns with symptomatic congenital cytomegalovirus infectionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was performed to assess oral valganciclovir V-GCV (GCV pro-drug), 15 mg/kg bid for 6 weeks to 13 neonates with symptomatic congenital cytomegalovirus (CMV). We monitored plasma levels of GCV within 30 days of therapy: Ctrough, and C2h (before and the 2 hours after administration), we performed viral assessment in plasma and urine and tolerability at baseline, and every fortnight. Pharmacokinetics showed GCV stable and effective plasma concentrations: mean Ctrough = 0.51 ± 0.3 and C2h : 3.81 ± 1.37 μg/ml. No significant variability was seen neither intra-patient nor inter-patients...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 18, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

In vitro activities of moxifloxacin and tigecycline against bacterial isolates associated with intraabdominal infections at a medical center in Taiwan, 2001–2006Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study found that moxifloxacin and tigecycline exhibited good in vitro activity against bacterial isolates causing IAIs. Content Type Journal ArticleCategory ArticleDOI 10.1007/s10096-009-0801-xAuthors C.-Y. Liu, National Taiwan University College of Medicine Department of Internal Medicine, National Taiwan University Hospital Taipei TaiwanC.-L. Lu, National Taiwan University College of Medicine Department of Internal Medicine, National Taiwan University Hospital Taipei TaiwanY.-T. Huang, National Taiwan University College of Medicine Department of Laboratory Medicine, National Taiwan University Hospital Taipei T...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 18, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Clonal spread of CC17 vancomycin-resistant Enterococcus faecium with multilocus sequence type 78 (ST78) and a novel ST444 in TaiwanEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract From May 2007 to January 2008, 30 isolates of vancomycin-resistant enterococci (VRE), including 29 Enterococcus faecium (96.7%) and 1 E. faecalis (3.3%) were obtained from various clinical specimens of 30 patients treated at a university hospital in Taiwan. Among these patients, 27 had VRE infections, including urinary tract infection (n = 16), bacteremia (n = 5), wound infection (n = 5), and central nervous system infection (n = 1). Three patients had VRE colonization. All of these isolates belonged to the vanA genotype with vancomycin minimum inhibitory concentrations of 64≥128 ...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 16, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

High stability of a new Granada medium agar that allows rapid and accurate detection of colonization with group B streptococci in pregnant womenEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Content Type Journal ArticleCategory Brief ReportDOI 10.1007/s10096-009-0811-8Authors T. Regnath, Laboratory Prof. G. Enders & Partners Rosenbergstrasse 85 70193 Stuttgart GermanyR. Ignatius, Charité Institute of Microbiology and Hygiene Spandauer Damm 130 14050 Berlin Germany Journal European Journal of Clinical Microbiology & Infectious DiseasesOnline ISSN 1435-4373Print ISSN 0934-9723
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 16, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Performance of Oxoid Brilliance™ MRSA medium for detection of methicillin-resistant Staphylococcus aureus: an in vitro studyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Oxoid Brilliance™ MRSA was evaluated for its ability to identify methicillin-resistant Staphylococcus aureus. A well-defined collection of staphylococci was used (n = 788). After 20 h incubation, the sensitivity was 99.6% and the specificity was 97.3%. This new medium is a highly sensitive method of screening for MRSA. Content Type Journal ArticleCategory ArticleDOI 10.1007/s10096-009-0802-9Authors E. Verkade, Amphia Hospital Laboratory for Microbiology and Infection Control P.O. Box 90158 4800 RK Breda The NetherlandsS. Elberts, Amphia Hospital Laboratory for Microbiology and Infection...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 16, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Clinical characteristics and outcomes of diabetic patients with Staphylococcus aureus bacteremia and endocarditisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The complications from S. aureus bacteremia (SAB) and infective endocarditis (SAIE) are higher in patients with diabetes. We summarize the characteristics and outcome of diabetic patients enrolled in a multicenter trial of daptomycin vs. standard therapy for SAB and SAIE. Adult patients with SAB were randomized to daptomycin 6 mg/kg/day or standard therapy (vancomycin 1 g every 12 h or antistaphylococcal penicillin 2 g every 4 h, both with gentamicin 1 mg/kg every 8 h for 4 days). Clinical success was defined as survival, resolution of S. aureus infection, and...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 16, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Development of a real-time PCR for the specific detection of Waddlia chondrophila in clinical samplesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Waddlia chondrophila is considered as an emerging human pathogen likely involved in miscarriage and lower respiratory tract infections. Given the low sensitivity of cell culture to recover such an obligate intracellular bacteria, molecular-based diagnostic approaches are warranted. We thus developed a real-time PCR that amplifies Waddlia chondrophila DNA. Specific primers and probe were selected to target the 16S rRNA gene. The PCR specifically amplified W. chondrophila but did not amplify other related-bacteria such as Parachlamydia acanthamoebae, Simkania negevensis and Chlamydia pneumoniae. The P...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 3, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Diagnostic value of procalcitonin in acutely hospitalized elderly patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The aim of this study was to evaluate procalcitonin as an adjunct to diagnose bacterial infections in older patients. One hundred seventy-two patients admitted to an acute-care geriatric unit during a 6-month period were prospectively included, 39 of them with an invasive bacterial infection. The best cut-off value to rule in a bacterial infection was 0.51 µg/l with sensitivity 64% and specificity 94%. The best cut-off value to rule out a bacterial infection was 0.08 µg/l with sensitivity 97% and specificity 20%. Procalcitonin was inconclusive (between 0.08 and 0.51 µg/l) for 112 admissions...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 3, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Antimicrobial stewardship program directed at broad-spectrum intravenous antibiotics prescription in a tertiary hospitalEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The antimicrobial stewardship program (ASP) is a major strategy to combat antimicrobial resistance and to limit its expenditure. We have improved on our existing ASP to implement a sustainable and cost-effective two-stage immediate concurrent feedback (ICF) model, in which the antimicrobial prescription is audited by two part-time infection control nurses at the first stage, followed by “physician ICF” at the second stage. In January 2005, an ASP focused on broad-spectrum intravenous antibiotics was implemented. All in-patients, except from the intensive care, bone marrow transplantation, liver ...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 3, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Adult community-acquired bacterial meningitis requiring ICU admission: epidemiological data, prognosis factors and adherence to IDSA guidelinesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Numerous guidelines are available to guide empirical antimicrobial therapy (EAT) in acute bacterial meningitis (ABM) patients. We analysed prognosis factors and compliance to the Infectious Diseases Society of America (IDSA) guidelines in ABM patients requiring stay in an intensive care unit (ICU). A 10-year retrospective study, using prospectively collected data, in 82 ABM patients admitted to a 16-bed university-affiliated French ICU was undertaken. Seventeen patients (20.7%) died during ICU stay. Multivariate analysis isolated four factors associated with in-ICU death: alcoholism (P = 0.007),...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 3, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Hepatitis C therapy—the future looks brightEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This article provides an overview of some of the small-molecule inhibitors that have shown promise so far in clinical trials and which could reach the clinic within the next three years. Content Type Journal ArticleCategory ReviewDOI 10.1007/s10096-009-0798-1Authors Sohail A. Qureshi, The Aga Khan University Hospital Department of Biological & Biomedical Sciences Stadium Road Karachi 74800 PakistanHumaira Qureshi, The Aga Khan University Hospital Department of Biological & Biomedical Sciences Stadium Road Karachi 74800 PakistanAnam Hameed, The Aga Khan University Hospital Medical College Stadium Road Karachi 74800 Pa...
Source: European Journal of Clinical Microbiology & Infectious Diseases - September 3, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Anti-NMDA receptor encephalitis: report of ten cases and comparison with viral encephalitisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study compares the presentation of such patients to those with viral encephalitis, so that infectious disease clinicians may identify individuals with this treatable disorder. Patients were physician-referred, and standardized forms were used to gather demographic, clinical, and laboratory data. Features of anti-NMDAR+ patients were compared with the viral encephalitides of enteroviral (EV), rabies, and herpes simplex-1 (HSV-1) origins. Sixteen cases with confirmed viral etiologies were all negative on NMDAR antibody testing. Ten anti-NMDAR+ patients were profiled with a median age of 18.5 years (range 11–...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 29, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Change in blood test after irradiation with high doses for inactivation of Lassa virusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we investigated if plasma and serum parameters are changed by irradiation with 5 kGy, 10 kGy, 15 kGy, 20 kGy or 40 kGy of 10 MeV electrons. For the 22 serum parameters measured there was no influence of radiation up to 20 kGy. Only at 40 kGy was a significant decrease noted. For the six plasma parameters the values were significantly dose dependent. To correct this a mathematical function was defined. It is possible to inactivate Lassa virus with high doses of radiation. Most of the measured blood values don’t change. For those which were influenced it was possibl...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 25, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Evaluation of antibiotic therapy following valve replacement for native valve endocarditisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective was to determine if outcomes differed based on whether they had received an antibiotic regimen recommended for native valve endocarditis or one for prosthetic valve endocarditis. Fifty-two patients had streptococcal and 53 had staphylococcal infections. Patients with each type of infection were divided into two groups: the first received postoperative monotherapy (with a beta-lactam or vancomycin), and the second received combination therapy (with an aminoglycoside for streptococcal infection, and gentamicin and/or rifampin for staphylococcal infection). The duration and types of antibiotics given pre- a...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 24, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Simultaneous detection of human bocavirus and adenovirus by multiplex real-time PCR in a Belgian paediatric populationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Since the discovery of human bocavirus (hBoV), the virus has been detected worldwide in respiratory tract samples from young children by various polymerase chain reaction (PCR) assays and real-time PCRs (Q-PCR). Until now, no data have been reported on the presence of hBoV in Belgium and the detection of hBoV in a multiplex Q-PCR setting has not been described. The aim of this study was to develop a fast and reliable multiplex Q-PCR for the simultaneous detection of hBoV DNA and adenovirus (AdV) DNA. During the winter of 2004–2005, 445 nasopharyngeal aspirates (NPAs) were analysed from 404 Belgian...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 24, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Prevalence and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) among pigs on German farms and import of livestock-related MRSA into hospitalsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The aim of this study was to evaluate the prevalence and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) among pigs and estimate the impact of this animal reservoir on human healthcare. Nasal swabs were derived from 1,600 pigs at 40 German farms. The MRSA were characterized using S. aureus protein A (spa) typing, multilocus sequence typing (MLST) and detection of toxin genes. In a retrospective case control study, we compared risk factors for the carriage of MRSA between patients carrying spa types found among regional pigs and patients with other MRSA molecular types. ...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 24, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Self-administered outpatient parenteral antimicrobial therapy: a report of three years experience in the Irish healthcare settingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Outpatient parenteral antibiotic therapy (OPAT) was first reported in 1972. OPAT programmes are not well established in Ireland, with no reported outcomes in the literature. An OPAT programme was established at St. James Hospital in 2006. Demographics, diagnoses and outcomes of the first 60 courses are reported. A retrospective analysis of prospectively recorded data was performed on patients treated from March 2006 to February 2009. The data was analysed using SPSS v.17. Sixty OPAT courses were administered to 56 patients, 57 percent of which were male. The median age was 50 years, the median ...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 21, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Intra-strain variability of methicillin-resistant Staphylococcus aureus strains ST228-MRSA-I and ST5-MRSA-IIEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Isolates belonging to two major epidemic strains of methicillin-resistant Staphylococcus aureus (MRSA) from clonal complex 5 were characterised using diagnostic microarrays in order to detect and analyse intra-strain variability. Isolates were sampled from hospitals scattered all over Germany. The study included 56 isolates of ST228-MRSA-I, which is also known as the South German Epidemic Strain, and 40 isolates of ST5-MRSA-II (UK-EMRSA-3, Rhine-Hesse Epidemic Strain, New York/Japan Clone), as well as, for comparison, some control strains and overseas isolates of ST5-MRSA-II. Both strains showed a r...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 20, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

A Chryseobacterium meningosepticum colonization outbreak in a neonatal intensive care unitEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion This study suggests that C. meningosepticum colonization in neonates does not necessarily lead to infection and that such colonization outbreaks may be controlled with emphasis on the standard precautions. Content Type Journal ArticleCategory ArticleDOI 10.1007/s10096-009-0797-2Authors S. Maraki, University Hospital of Heraklion Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine P.O. Box 1352 71110 Heraklion Crete GreeceE. Scoulica, University Hospital of Heraklion Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine P.O. B...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 15, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Hemolytic uremic syndrome (HUS)—incidence and etiologies at a regional Children’s Hospital in 2001–2006Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study investigated the incidence of HUS at a regional Children’s Hospital between 2001 and 2006 by retrospective review. Cases of HUS were investigated for outcomes based on stool culture and an association of acute pancreatitis. A total of 44 cases were identified, of which 57% were female and 43% were male, with an age distribution of 13 months to 17 years and a median age of 3.44 years. Data revealed 13 cases in 2006 compared to two cases in 2001, with 84% of all illnesses occurring in the summer and fall seasons. The median duration of thrombocytopenia was eight days and 50% of all cases requ...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 15, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Real-time PCR strategy and detection of bacterial agents of lymphadenitisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The aim of this study was to compare 16 S rRNA gene amplification and sequencing with a systematic real-time PCR assay screening strategy that includes all common known pathogens recovered from lymph node biopsy specimens. Lymph node biopsy samples sent to our laboratory from January 2007 to December 2008 were tested in the study. Lymph nodes were screened for the presence of any bacteria by PCR amplification and sequencing targeting the 16 S rRNA gene and also by a specific real-time PCR strategy that includes Bartonella henselae, mycobacteria, Francisella tularensis, and Tropheryma whipp...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 14, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Late infectious complications after cord blood stem cell transplantationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, survival beyond day 100 following cord bloodstem cell transplantation is associated with a considerable risk of infections in our single center experience. Content Type Journal ArticleCategory Brief ReportDOI 10.1007/s10096-009-0789-2Authors N. G. Almyroudis, Roswell Park Cancer Institute Department of Medicine Elm and Carlton Streets Buffalo NY 14263 USAJ. Fabian, Roswell Park Cancer Institute Department of Medicine Elm and Carlton Streets Buffalo NY 14263 USAT. Hahn, Roswell Park Cancer Institute Department of Medicine Elm and Carlton Streets Buffalo NY 14263 USAB. H. Segal, Roswell Park Cancer Insti...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 11, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

A 1-minute hand wash does not impair the efficacy of a propanol-based hand rub in two consecutive surgical hand disinfection proceduresEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract We studied the effect of a 1-min hand wash on the bacterial hand flora in two consecutive surgical hand disinfection procedures. A propanol-based hand rub (PBHR; Sterillium®) and n-propanol (60%, v/v) were tested in a Latin-square design according to EN 12791 in four variations. The reference alcohol was always applied for 3 min after a 1-min hand wash (variation 1). The PBHR was applied for 1.5 min (first application) or 0.5 min (second application). Variation 2 included a 1-min hand wash before both applications, variation 3 included the hand wash before application 1, in variation...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 11, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Cost-effectiveness of rapid MRSA screening in surgical patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study investigates the effectiveness of a same-day polymerase chain reaction (PCR) test for the rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) in a general screening of patients admitted to the trauma surgery and heart surgery department in a German university hospital. A total of 442 patients were screened over a 4-month period by using a PCR assay, compared to culture methods, for specimens from the nose and throat. The MRSA carriage rate on admission was 3.85% during the study period. The PCR results of 1,680 swabs showed a sensitivity of 85% and a specificity of 99.39% for swabs from the...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 9, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Statins are associated with improved outcomes of bloodstream infection in solid-organ transplant recipientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, appropriate antibiotic therapy and statin use are associated with lower risk of mortality from BSIs in this patient population. Content Type Journal ArticleCategory ArticleDOI 10.1007/s10096-009-0787-4Authors J. Hsu, University of Wisconsin Section of Infectious Diseases, Department of Medicine Room H4/572 CSC, 600 Highland Avenue Madison WI 53792 USAD. R. Andes, University of Wisconsin Section of Infectious Diseases, Department of Medicine Room H4/572 CSC, 600 Highland Avenue Madison WI 53792 USAV. Knasinski, University of Wisconsin Division of Transplantation, Department of Surgery Madison WI USAJ. P...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 9, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Epidemiology of invasive neonatal Cronobacter (Enterobacter sakazakii) infectionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract About 120–150 neonatal Cronobacter spp. (Enterobacter sakazakii) infections have been described. An analysis of current case numbers, epidemiological measures and risk factors is warranted. Data of microbiologically confirmed cases, published between 2000 and 2008, have been analysed statistically. More than 100 neonatal Cronobacter infections have been reported in this period. The overall lethality of the 67 invasive infections was 26.9%. The lethality of Cronobacter meningitis, bacteraemia and necrotising enterocolitis (NEC) was calculated to be 41.9% (P < 0.0001), <10% and 19.0% (P &...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 7, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Sexually transmitted infections in tribal populations of central IndiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract This community-based cross-sectional study was carried out in 17 tribal villages of the Kundam block of the Jabalpur district of India. Individuals with sexually transmitted disease (STD) syndromes were enumerated and the specimens were collected for the laboratory diagnosis of sexually transmitted infections (STIs). Trichomoniasis, gonorrhoea, bacterial vaginosis and syphilis sero-reactivity were diagnosed by standard microbiological techniques. Chlamydia infection was detected by using polymerase chain reaction (PCR). A definite laboratory diagnosis of STIs could be established in 36.5% individual...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 5, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

A recombinant hybrid peptide composed of AAF adhesin of enteroaggregative Escherichia coli and Shiga toxin B subunit elicits protective immune response in miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Shiga toxin producing Escherichia coli (STEC) are a group of diarrheagenic Escherichia coli (E. coli) whereby Shiga toxin is the main virulence factor. It is composed of an A subunit, which mediates toxicity, and a B subunit (StxB), which is a nontoxic homopentameric protein responsible for toxin binding and internalization into target cells by interacting with the glycolipid, globotriaosylceramide (Gb3). Enteroaggregative Escherichia coli (EAEC) are a group of E. coli with aggregative adherence to epithelial cells, which play an important role in its pathogenesis. EAEC are the cause of diarrhea in d...
Source: European Journal of Clinical Microbiology & Infectious Diseases - August 4, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

The effect of Bacillus subtilis mouth rinsing in patients with periodontitisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Bacillus subtilis is an effective probiotic product for prevention of enteric infections both in humans and animals. We hypothesized that a mouth rinse containing Bacillus subtilis should adhere to and colonize part of the oral bacteria on periodontal tissue. The rinsing ability of Extraction 300E (containing Bacillus subtilis: E-300 ) was compared with that of a mouth wash liquid , Neosteline Green (benzethonium chloride; NG) that is commonly used in Japan. Compared with NG rinsing, E-300 rinsing resulted in a marked change in the BANA-score. The mean BANA values (score ± SD) over the course of ...
Source: European Journal of Clinical Microbiology & Infectious Diseases - July 31, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Prevalence of antibodies against Ascaris suum and its association with allergic manifestations in 4-year-old children in the Netherlands: the PIAMA birth cohort studyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aims at determining the prevalence of antibodies against A. suum and its association with allergic symptoms and sensitisation in a population of 4-year-old children living in the Netherlands. Blood samples from 629 children from the prospective birth cohort Prevention and Incidence of Asthma and Mite Allergy (PIAMA) study were examined for Ascaris antibodies. Data on allergic symptoms and sensitisation were collected using questionnaires and radioallergosorbent tests (RAST). A total of 45 out of 629 (7%) were found to be Ascaris-seropositive. In addition, a positive association between Ascaris seropositivity ...
Source: European Journal of Clinical Microbiology & Infectious Diseases - July 30, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Antimicrobial non-susceptibility of cervico-vaginal and rectal Escherichia coli isolates is associated with phylogeny and plasmid carriageEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Uropathogenic Escherichia coli (UPEC) is the primary cause of urinary tract infections (UTIs) in adult women, which are increasingly refractory to antimicrobial treatment. UPEC colonizes the vagina prior to causing a UTI. Our hypothesis was that the vaginal flora would be enriched in UPEC and therefore have a greater prevalence of non-susceptibility relative to the rectal flora. We used disk diffusion to determine the antimicrobial susceptibilities of 100 cervico-vaginal E. coli (CVEC) and 100 rectal E. coli (REC) isolates from 200 different patients. Phylogeny, plasmid replicons, and antimicrobial r...
Source: European Journal of Clinical Microbiology & Infectious Diseases - July 29, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Detection of Helicobacter pylori, Enterococcus faecalis, and Pseudomonas aeruginosa in the subgingival biofilm of HIV-infected subjects undergoing HAART with chronic periodontitisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study compared the frequency of Helicobacter pylori, Enterococcus faecalis, and Pseudomonas aeruginosa in the subgingival microbiota of HIV-seropositive and HIV-seronegative subjects with periodontitis or clinically healthy periodontal tissues. Fifty-four subjects were distributed into two HIV-seropositive groups (chronic periodontitis [HCP = 13] and periodontal health [HH = 10]) and two HIV-seronegative groups (chronic periodontitis [CP = 17] and periodontal health [H = 14]). The detection of bacterial species was carried out by polymerase chain reaction (PCR). CP patients showed significantly more periodontal de...
Source: European Journal of Clinical Microbiology & Infectious Diseases - July 28, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Outbreak of multiresistant Acinetobacter baumannii in a polyvalent intensive care unit: clinical, epidemiological analysis and PFGE-printing evolutionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract An outbreak of multidrug-resistant Acinetobacter baumannii (MRAB) occurred over the course of a 27-week period in our adult polyvalent intensive care unit (ICU). Twenty-one patients were affected, and 72 strains were identified from different clinical samples. The strains were resistant to all antibiotics except for colistin and ampicillin/sulbactam. Forty-nine MRAB strains collected from 18 patients were analysed by pulsed-field gel electrophoresis (PFGE). This analysis revealed four highly-related PFGE types (genetic similarity index >90%) termed 1, 2, 3 and 4, that were isolated in 13, seven, ...
Source: European Journal of Clinical Microbiology & Infectious Diseases - July 28, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Evaluation of a commercial MRSA assay when multiple MRSA strains are causing epidemicsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Rapid and reliable diagnostic methods are needed to control methicillin-resistant Staphylococcus aureus (MRSA) transmission. We studied the BD GeneOhm™ MRSA Assay which is based on one specific amplification product at the junction of the right extremity sequence of the staphylococcal cassette chromosome mec (SCCmec) and the chromosomal sequence of orfX of S. aureus. The test was applied on 95 clinical isolates in Finland: 83% were positive. The isolates giving negative results represented several pulsed-field gel electrophoresis (PFGE) types and harboured SCCmec types IV, V, VI or were new types wi...
Source: European Journal of Clinical Microbiology & Infectious Diseases - July 26, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Feasibility of collecting self-sampled vaginal swabs by mail: quantity and quality of genomic DNAEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, the large majority of mailed self-sampled vaginal swabs resulted in DNA of adequate purity and concentration for further research. Content Type Journal ArticleCategory ArticleDOI 10.1007/s10096-009-0776-7Authors M. F. D. Baay, University of Antwerp (CDE, T3) Laboratory for Cancer Research and Clinical Oncology Universiteitsplein 1 2610 Wilrijk BelgiumV. Verhoeven, University of Antwerp Centre for General Practice Wilrijk BelgiumH. A. J. Lambrechts, University of Antwerp (CDE, T3) Laboratory for Cancer Research and Clinical Oncology Universiteitsplein 1 2610 Wilrijk BelgiumG. G. O. Pattyn, University of...
Source: European Journal of Clinical Microbiology & Infectious Diseases - July 23, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Costs and benefits of the MRSA Search and Destroy policy in a Dutch hospitalEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, application of the MRSA Search and Destroy policy in a hospital in a country with a low endemic MRSA incidence saves money and lives. Content Type Journal ArticleCategory ArticleDOI 10.1007/s10096-009-0775-8Authors M. M. L. van Rijen, Amphia Hospital Breda Laboratory for Microbiology and Infection Control Langendijk 75 P.O. Box 90157 4800 RL Breda The NetherlandsJ. A. J. W. Kluytmans, Amphia Hospital Breda Laboratory for Microbiology and Infection Control P.O. Box 90158 4800 RK Breda The Netherlands Journal European Journal of Clinical Microbiology & Infectious DiseasesOnline ISSN 1435-4373Print ISSN 0934-9723
Source: European Journal of Clinical Microbiology & Infectious Diseases - July 22, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Combination antibiotic susceptibility of biofilm-grown Burkholderia cepacia and Pseudomonas aeruginosa isolated from patients with pulmonary exacerbations of cystic fibrosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study identified potentially effective antibiotic combinations to guide the empirical treatment of CF pulmonary exacerbations. Content Type Journal ArticleCategory Brief ReportDOI 10.1007/s10096-009-0774-9Authors L. Dales, University of Ottawa The Ottawa Health Research Institute Ottawa ON CanadaW. Ferris, Children’s Hospital of Eastern Ontario Ottawa ON CanadaK. Vandemheen, University of Ottawa The Ottawa Health Research Institute Ottawa ON CanadaS. D. Aaron, University of Ottawa The Ottawa Health Research Institute Ottawa ON Canada Journal European Journal of Clinical Microbiology & Infectious Disease...
Source: European Journal of Clinical Microbiology & Infectious Diseases - July 6, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Distribution of pathogenic Yersinia enterocolitica in ChinaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Yersinia enterocolitica (1,295 strains) was isolated from diarrhea patients, livestock, poultry, wild animals, insect vectors, food, and the environment. They were studied for epidemiology distribution using bacterial biochemical metabolism tests, their virulence genes, and pulsed-field gel electrophoresis (PFGE) sub-typing. The data showed that 416 of the 1,295 strains were pathogenic, where the pathogenic Chinese isolates were of serotypes O:3 and O:9. These two serotypes were found in livestock and poultry, with swine serving as the major reservoir. The geographic distribution of pathogenic iso...
Source: European Journal of Clinical Microbiology & Infectious Diseases - July 6, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Retrospective analysis of the genetic diversity of Klebsiella oxytoca isolated in Poland over a 50-year periodEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, we found that the ITS-PCR and PCR MP methods are useful for the phylogenetic delineation of genetic groups in K. oxytoca. Content Type Journal ArticleCategory Brief ReportDOI 10.1007/s10096-009-0768-7Authors K. Stojowska, Gdańsk University of Technology Department of Microbiology ul. G. Narutowicza 11/12 80-233 Gdańsk PolandB. Krawczyk, Gdańsk University of Technology Department of Microbiology ul. G. Narutowicza 11/12 80-233 Gdańsk PolandS. Kałużewski, National Institute of Public Health, National Institute of Hygiene Department of Bacteriology ul. Chocimska 24 00-791 Warszawa PolandJ. Kur, Gda�...
Source: European Journal of Clinical Microbiology & Infectious Diseases - June 29, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

Comparison of two PCR-based methods and conventional culture for the detection of nasal carriage of Staphylococcus aureus in pre-operative patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Nasal carriage is an important risk factor for the development of post-operative infections with Staphylococcus aureus and pre-operative treatment with mupirocin in carriers reduces the post-operative infection rate. Therefore, it is important to identify nasal carriage rapidly. Two polymerase chain reaction (PCR) techniques were compared to conventional culture in surgical patients. In 404 consecutive patients, nasal swabs were taken for pre-operative screening for the nasal carriage of S. aureus. The performance of the Roche Staphylococcus Kit on Lightcycler (Roche; RSA) and the Becton Dickinson (S...
Source: European Journal of Clinical Microbiology & Infectious Diseases - June 29, 2009 Category: Microbiology Tags: European Journal of Clinical Microbiology & Infectious Diseases Source Type: journals

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