Combining SARS-CoV-2 interferon-gamma release assay with humoral response assessment to define immune memory profiles
CONCLUSION: The discrepancy between humoral and cellular responses highlights the significance of assessing the overall adaptive immune response. This integrated approach allows the identification of vulnerable subjects and can be of interest to guide antiviral prophylaxis at an individual level.PMID:38627984 | DOI:10.1002/eji.202451035 (Source: European Journal of Immunology)
Source: European Journal of Immunology - April 17, 2024 Category: Allergy & Immunology Authors: William Mouton Guy Oriol Christelle Compagnon Carla Saade Kahina Saker Priscille Franc Bouchra Mokdad Aurore Fleurie Xavier Lacoux Soizic Daniel Franck Berthier C écile Barnel Bruno Pozzetto Jean-Baptiste Fassier Val érie Dubois Sophia Djebali Maxence D Source Type: research
Canonical and noncanonical functions of complement in systemic lupus erythematosus
Eur J Immunol. 2024 Apr 17:e2350918. doi: 10.1002/eji.202350918. Online ahead of print.ABSTRACTFor many years complement activation in systemic lupus erythematosus (SLE) was viewed as a major cause of tissue injury. However, human and murine studies showed that complement plays a protective as well as a proinflammatory role in tissue damage. A hierarchy is apparent with early classical pathway components, particularly C1q, exerting the greatest influence. Understanding the mechanisms underlying the protective function(s) of complement remains an important challenge for the future and has implications for the use of complem...
Source: European Journal of Immunology - April 17, 2024 Category: Allergy & Immunology Authors: Matthew C Pickering Marina Botto Source Type: research
Empowering γδ T-cell functionality with vitamin C
Eur J Immunol. 2024 Apr 15:e2451028. doi: 10.1002/eji.202451028. Online ahead of print.ABSTRACTVitamin C (ascorbic acid) is a potent antioxidant and a cofactor for various enzymes including histone demethylases and methylcytosine dioxygenases. Vitamin C also exerts direct cytotoxicity toward selected tumor cells including colorectal carcinoma. Moreover, vitamin C has been shown to impact immune cell differentiation at various levels including maturation and/or functionality of T cells and their progenitors, dendritic cells, B cells, and NK cells. γδ T cells have recently attracted great interest as effector cells for cel...
Source: European Journal of Immunology - April 15, 2024 Category: Allergy & Immunology Authors: Dieter Kabelitz Lea Cierna Claudia Juraske Michal Zarobkiewicz Wolfgang W Schamel Christian Peters Source Type: research
Empowering γδ T-cell functionality with vitamin C
Eur J Immunol. 2024 Apr 15:e2451028. doi: 10.1002/eji.202451028. Online ahead of print.ABSTRACTVitamin C (ascorbic acid) is a potent antioxidant and a cofactor for various enzymes including histone demethylases and methylcytosine dioxygenases. Vitamin C also exerts direct cytotoxicity toward selected tumor cells including colorectal carcinoma. Moreover, vitamin C has been shown to impact immune cell differentiation at various levels including maturation and/or functionality of T cells and their progenitors, dendritic cells, B cells, and NK cells. γδ T cells have recently attracted great interest as effector cells for cel...
Source: European Journal of Immunology - April 15, 2024 Category: Allergy & Immunology Authors: Dieter Kabelitz Lea Cierna Claudia Juraske Michal Zarobkiewicz Wolfgang W Schamel Christian Peters Source Type: research
Impressum
Eur J Immunol. 2024 Apr;54(4):e2470043. doi: 10.1002/eji.202470043.NO ABSTRACTPMID:38609690 | DOI:10.1002/eji.202470043 (Source: European Journal of Immunology)
Source: European Journal of Immunology - April 12, 2024 Category: Allergy & Immunology Source Type: research
Vitamin A-treated natural killer cells reduce interferon-gamma production and support regulatory T-cell differentiation
Eur J Immunol. 2024 Apr 9:e2250342. doi: 10.1002/eji.202250342. Online ahead of print.ABSTRACTNatural killer (NK) cells are innate cytotoxic lymphocytes that contribute to immune responses against stressed, transformed, or infected cells. NK cell effector functions are regulated by microenvironmental factors, including cytokines, metabolites, and nutrients. Vitamin A is an essential micronutrient that plays an indispensable role in embryogenesis and development, but was also reported to regulate immune responses. However, the role of vitamin A in regulating NK cell functions remains poorly understood. Here, we show that th...
Source: European Journal of Immunology - April 9, 2024 Category: Allergy & Immunology Authors: Mingeum Jeong Francesco Cortopassi Jia-Xiang See Carolina De La Torre Adelheid Cerwenka Ana Stojanovic Source Type: research
Proinflammatory polarization strongly reduces human macrophage in vitro phagocytosis of tumor cells in response to CD47 blockade
Eur J Immunol. 2024 Apr 9:e2350824. doi: 10.1002/eji.202350824. Online ahead of print.ABSTRACTAntibody-based CD47 blockade aims to activate macrophage phagocytosis of tumor cells. However, macrophages possess a high degree of phenotype heterogeneity that likely influences phagocytic capacity. In murine models, proinflammatory (M1) activation increases macrophage phagocytosis of tumor cells, but in human models, results have been conflicting. Here, we investigated the effects of proinflammatory polarization on the phagocytic response of human monocyte-derived macrophages in an in vitro model. Using both flow cytometry-based...
Source: European Journal of Immunology - April 9, 2024 Category: Allergy & Immunology Authors: Kristian W Antonsen Anne G Jensen Mikkel Carstensen Lene N Nejsum Boe S Sorensen Anders Etzerodt S øren K Moestrup Holger J M øller Source Type: research
Predominance of M2 macrophages in organized thrombi in chronic thromboembolic pulmonary hypertension patients
Eur J Immunol. 2024 Apr 9:e2350670. doi: 10.1002/eji.202350670. Online ahead of print.ABSTRACTChronic thromboembolic pulmonary hypertension (CTEPH) is a debilitating disease characterized by thrombotic occlusion of pulmonary arteries and vasculopathy, leading to increased pulmonary vascular resistance and progressive right-sided heart failure. Thrombotic lesions in CTEPH contain CD68+ macrophages, and increasing evidence supports their role in disease pathogenesis. Macrophages are classically divided into pro-inflammatory M1 macrophages and anti-inflammatory M2 macrophages, which are involved in wound healing and tissue re...
Source: European Journal of Immunology - April 9, 2024 Category: Allergy & Immunology Authors: Thomas Koudstaal Thierry van den Bosch Ingrid Bergen Karishma Lila Paul Bresser Harm Jan Bogaard Karin Boomars Rudi Hendriks Jan von der Th üsen Source Type: research
Diversification of the VH3-53 immunoglobulin gene segment by somatic hypermutation results in neutralization of SARS-CoV-2 virus variants
Eur J Immunol. 2024 Apr 9:e2451056. doi: 10.1002/eji.202451056. Online ahead of print.ABSTRACTCOVID-19 induces re-circulating long-lived memory B cells (MBC) that, upon re-encounter with the pathogen, are induced to mount immunoglobulin responses. During convalescence, antibodies are subjected to affinity maturation, which enhances the antibody binding strength and generates new specificities that neutralize virus variants. Here, we performed a single-cell RNA sequencing analysis of spike-specific B cells from a SARS-CoV-2 convalescent subject. After COVID-19 vaccination, matured infection-induced MBC underwent recall and ...
Source: European Journal of Immunology - April 9, 2024 Category: Allergy & Immunology Authors: Matthias Bruhn Maureen Obara Abdus Salam Bibiana Costa Annett Ziegler Inken Waltl Andreas Pavlou Markus Hoffmann Theresa Graalmann Stefan P öhlmann Axel Schambach Ulrich Kalinke Source Type: research
Vitamin A-treated natural killer cells reduce interferon-gamma production and support regulatory T-cell differentiation
Eur J Immunol. 2024 Apr 9:e2250342. doi: 10.1002/eji.202250342. Online ahead of print.ABSTRACTNatural killer (NK) cells are innate cytotoxic lymphocytes that contribute to immune responses against stressed, transformed, or infected cells. NK cell effector functions are regulated by microenvironmental factors, including cytokines, metabolites, and nutrients. Vitamin A is an essential micronutrient that plays an indispensable role in embryogenesis and development, but was also reported to regulate immune responses. However, the role of vitamin A in regulating NK cell functions remains poorly understood. Here, we show that th...
Source: European Journal of Immunology - April 9, 2024 Category: Allergy & Immunology Authors: Mingeum Jeong Francesco Cortopassi Jia-Xiang See Carolina De La Torre Adelheid Cerwenka Ana Stojanovic Source Type: research
Proinflammatory polarization strongly reduces human macrophage in vitro phagocytosis of tumor cells in response to CD47 blockade
Eur J Immunol. 2024 Apr 9:e2350824. doi: 10.1002/eji.202350824. Online ahead of print.ABSTRACTAntibody-based CD47 blockade aims to activate macrophage phagocytosis of tumor cells. However, macrophages possess a high degree of phenotype heterogeneity that likely influences phagocytic capacity. In murine models, proinflammatory (M1) activation increases macrophage phagocytosis of tumor cells, but in human models, results have been conflicting. Here, we investigated the effects of proinflammatory polarization on the phagocytic response of human monocyte-derived macrophages in an in vitro model. Using both flow cytometry-based...
Source: European Journal of Immunology - April 9, 2024 Category: Allergy & Immunology Authors: Kristian W Antonsen Anne G Jensen Mikkel Carstensen Lene N Nejsum Boe S Sorensen Anders Etzerodt S øren K Moestrup Holger J M øller Source Type: research
Predominance of M2 macrophages in organized thrombi in chronic thromboembolic pulmonary hypertension patients
Eur J Immunol. 2024 Apr 9:e2350670. doi: 10.1002/eji.202350670. Online ahead of print.ABSTRACTChronic thromboembolic pulmonary hypertension (CTEPH) is a debilitating disease characterized by thrombotic occlusion of pulmonary arteries and vasculopathy, leading to increased pulmonary vascular resistance and progressive right-sided heart failure. Thrombotic lesions in CTEPH contain CD68+ macrophages, and increasing evidence supports their role in disease pathogenesis. Macrophages are classically divided into pro-inflammatory M1 macrophages and anti-inflammatory M2 macrophages, which are involved in wound healing and tissue re...
Source: European Journal of Immunology - April 9, 2024 Category: Allergy & Immunology Authors: Thomas Koudstaal Thierry van den Bosch Ingrid Bergen Karishma Lila Paul Bresser Harm Jan Bogaard Karin Boomars Rudi Hendriks Jan von der Th üsen Source Type: research
Diversification of the VH3-53 immunoglobulin gene segment by somatic hypermutation results in neutralization of SARS-CoV-2 virus variants
Eur J Immunol. 2024 Apr 9:e2451056. doi: 10.1002/eji.202451056. Online ahead of print.ABSTRACTCOVID-19 induces re-circulating long-lived memory B cells (MBC) that, upon re-encounter with the pathogen, are induced to mount immunoglobulin responses. During convalescence, antibodies are subjected to affinity maturation, which enhances the antibody binding strength and generates new specificities that neutralize virus variants. Here, we performed a single-cell RNA sequencing analysis of spike-specific B cells from a SARS-CoV-2 convalescent subject. After COVID-19 vaccination, matured infection-induced MBC underwent recall and ...
Source: European Journal of Immunology - April 9, 2024 Category: Allergy & Immunology Authors: Matthias Bruhn Maureen Obara Abdus Salam Bibiana Costa Annett Ziegler Inken Waltl Andreas Pavlou Markus Hoffmann Theresa Graalmann Stefan P öhlmann Axel Schambach Ulrich Kalinke Source Type: research
Vitamin A-treated natural killer cells reduce interferon-gamma production and support regulatory T-cell differentiation
Eur J Immunol. 2024 Apr 9:e2250342. doi: 10.1002/eji.202250342. Online ahead of print.ABSTRACTNatural killer (NK) cells are innate cytotoxic lymphocytes that contribute to immune responses against stressed, transformed, or infected cells. NK cell effector functions are regulated by microenvironmental factors, including cytokines, metabolites, and nutrients. Vitamin A is an essential micronutrient that plays an indispensable role in embryogenesis and development, but was also reported to regulate immune responses. However, the role of vitamin A in regulating NK cell functions remains poorly understood. Here, we show that th...
Source: European Journal of Immunology - April 9, 2024 Category: Allergy & Immunology Authors: Mingeum Jeong Francesco Cortopassi Jia-Xiang See Carolina De La Torre Adelheid Cerwenka Ana Stojanovic Source Type: research
Proinflammatory polarization strongly reduces human macrophage in vitro phagocytosis of tumor cells in response to CD47 blockade
Eur J Immunol. 2024 Apr 9:e2350824. doi: 10.1002/eji.202350824. Online ahead of print.ABSTRACTAntibody-based CD47 blockade aims to activate macrophage phagocytosis of tumor cells. However, macrophages possess a high degree of phenotype heterogeneity that likely influences phagocytic capacity. In murine models, proinflammatory (M1) activation increases macrophage phagocytosis of tumor cells, but in human models, results have been conflicting. Here, we investigated the effects of proinflammatory polarization on the phagocytic response of human monocyte-derived macrophages in an in vitro model. Using both flow cytometry-based...
Source: European Journal of Immunology - April 9, 2024 Category: Allergy & Immunology Authors: Kristian W Antonsen Anne G Jensen Mikkel Carstensen Lene N Nejsum Boe S Sorensen Anders Etzerodt S øren K Moestrup Holger J M øller Source Type: research
