Experimental DermatologyThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

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A simple assay for the study of human hair follicle damage induced by ionizing irradiationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: A simple assay for the study of human hair follicle damage induced by ionizing irradiation. Experimental Dermatology 2009.Abstract: Due to its rapidly proliferating matrix keratinocytes, the hair follicle is highly sensitive to ionizing irradiation (IR)-induced skin damage and thus an instructive and clinically relevant model organ for investigating the effects of IR on rapidly dividing epithelial-mesenchymal interaction systems. Here, we have assessed the impact of IR on organ-cultured human scalp hair follicles. We show that IR significantly inhibits the proliferation and induces apoptosis of h...
Source: Experimental Dermatology - November 19, 2009 Category: Dermatology Authors: Burkhard Poeggeler, Enikö Bodó, Roger Nadrowitz, Juergen Dunst, Ralf Paus Source Type: journals

The mineralocorticoid receptor as a novel player in skin biology: beyond the renal horizon?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: The mineralocorticoid receptor as a novel player in skin biology: beyond the renal horizon? Experimental Dermatology 2009.Abstract: The mineralocorticoid receptor (MR) and its ligand aldosterone regulate renal sodium reabsorption and blood pressure and much knowledge has been accumulated in MR physiopathology, cellular and molecular targets. In contrast, our understanding of this hormonal system in non-classical targets (heart, blood vessels, neurons, keratinocytes[hellip]) is limited, particularly in the mammalian skin. We review here the few available data that point on MR in the skin and that ...
Source: Experimental Dermatology - November 18, 2009 Category: Dermatology Authors: Nicolette Farman, Eve Maubec, Burkhard Poeggeler, Jennifer E. Klatte, Frederic Jaisser, Ralf Paus Source Type: journals

Can blood components with age-related changes influence the ageing of endothelial cells?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, our data suggest that blood components that exhibit age-related changes, such as alterations in cytoskeletal regulators and stress proteins, may be associated with endothelial cell ageing. (Source: Experimental Dermatology)
Source: Experimental Dermatology - November 18, 2009 Category: Dermatology Authors: Ji Yeon Noh, Sang Ho Oh, Ju Hee Lee, Yeon Sook Kwon, Dong Jin Ryu, Kwang Hoon Lee Source Type: journals

In vivo synthesis and secretion of erythropoietin by genetically modified primary human keratinocytes grafted onto immunocompromised miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Keratinocytes carry the potential to serve as a genetically modified biofactory synthesizing human EPO. In vivo gene selection does not allow to select for increased EPO secretion, most likely because of altered secretory activity of transduced KC in the stratified, differentiated epidermis. Thus, further studies are necessary to optimize the release of EPO by genetically modified KC. (Source: Experimental Dermatology)
Source: Experimental Dermatology - November 3, 2009 Category: Dermatology Authors: Frank Scheidemann, Jean-Philippe Therrien, Jon Vogel, Wolfgang Pfützner Source Type: journals

CD40 signalling induces IL-10-producing, tolerogenic dendritic cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to characterize the role of the CD40-CD40L pathway for differentiation and function of human DC. Therefore, iDC were stimulated through CD40-CD40L interaction by transduction of DC with adenoviral vectors encoding for CD40L (Ad-CD40L). Resulting DC (CD40L-DC) were analysed concerning their phenotype, cytokine profile and T cell stimulatory capacity. Transduction induced a DC phenotype comparable to stimulation with proinflammatory cytokines as revealed by upregulation of CD83 and the costimulatory molecules CD80 and CD86. Additionally, Ad-CD40L-induced strong production of IL-12p70 not observed in c...
Source: Experimental Dermatology - November 2, 2009 Category: Dermatology Authors: Andrea Tuettenberg, Sabine Fondel, Kerstin Steinbrink, Alexander H. Enk, Helmut Jonuleit Source Type: journals

Reflectance confocal microscopy for pigmentary disordersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This article reviewed the RCM applications in the characterization and management of pigmentary disorders. The application of RCM in pigmentary disorders has been expanded to describe hyper- and hypopigmentary disorders as well as pigmented skin tumors. The great advantages of non-invasive and repetitive examination of RCM may provide its usefulness not only in the diagnosis and management of pigmentary disorders, but also in researching pathogenesis of pigmentary disorders. (Source: Experimental Dermatology)
Source: Experimental Dermatology - November 2, 2009 Category: Dermatology Authors: Hee Young Kang, Philippe Bahadoran, Jean-Paul Ortonne Source Type: journals

Increased alpha-melanocyte-stimulating hormone (α-MSH) levels and melanocortin receptors expression associated with pigmentation in an NC/Nga mouse model of atopic dermatitisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Increased alpha-melanocyte-stimulating hormone ([alpha]-MSH) levels and melanocortin receptors expression associated with pigmentation in an NC/Nga mouse model of atopic dermatitis. Experimental Dermatology 2009.Abstract: Patients with a specific subtype of atopic dermatitis (AD) display particular patterns of pigmentation, such as ripple pattern pigmentation on the neck, pigmented macules on the lip and diffuse pigmentation. However, the mechanism underlying these patterns has not been determined. The purpose of our research is to investigate the factors influencing this type of pigmentation in ...
Source: Experimental Dermatology - November 2, 2009 Category: Dermatology Authors: Keiichi Hiramoto, Hiromi Kobayashi, Masamitsu Ishii, Eisuke Sato, Masayasu Inoue Source Type: journals

Increased mast cell expression of PAR-2 in skin inflammatory diseases and release of IL-8 upon PAR-2 activationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, skin biopsies from both healthy-looking and lesional skin of patients with psoriasis and superficial spreading BCC were collected and the expression of PAR-2 immunoreactivity in tryptase-positive mast cells was analysed. PAR-2 expression was confirmed in vitro in different mast cell populations. Cord-blood derived mast cells (CBMC) were stimulated with a PAR-2 activating peptide, 2-furoyl-LIGRLO-NH2. Consequently, IL-8 and histamine production was analysed in the supernatants. We observed a significant increase in the percentage of mast cells expressing PAR-2 in the lesional skin of psoriasis and BCC patient...
Source: Experimental Dermatology - November 2, 2009 Category: Dermatology Authors: Ricardo Filipe da Silva Carvalho, Gunnar Nilsson, Ilkka Tapani Harvima Source Type: journals

Topical treatment with the vitamin D analogue calcipotriol enhances the upregulation of the antimicrobial protein hCAP18/LL-37 during wounding in human skin in vivoEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Topical treatment with the vitamin D analogue calcipotriol enhances the upregulation of the antimicrobial protein hCAP18/LL-37 during wounding in human skin in vivo. Experimental Dermatology 2009.Abstract: Cathelicidin antimicrobial protein, hCAP18, is the sole cathelin protein in human. Its C-terminal peptide, which is released enzymatically from the holoprotein, has broad antimicrobial activity but also has effects on eukaryotic cells. hCAP18 is present in leukocytes and is produced at epithelial interfaces as part of the innate immune system. In normal intact skin, there is low constitutive ex...
Source: Experimental Dermatology - October 29, 2009 Category: Dermatology Authors: Johan D. Heilborn, Günther Weber, Alvar Grönberg, Christine Dieterich, Mona Ståhle Source Type: journals

Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice. Experimental Dermatology 2009.Abstract: The diarylheptanoid, oregonin (ORE), which was isolated from the bark of Alnus japonica Steudel that grows natively in Korea, has been known to exert antioxidative, anti-inflammatory, anti-cancer and immune response inhibitory effects. The antioxidative effect of ORE was observed on the superoxide and 1,1-diphenyl-2-picrylhydrazyl radical, as well as on the expression of inducible nitric oxide synthase and cyclooxygenase-2 in lipopolysaccharide-treat...
Source: Experimental Dermatology - October 21, 2009 Category: Dermatology Authors: Sun Eun Choi, Mi Sook Jeong, Myung Joo Kang, Do Ik Lee, Seong Soo Joo, Chung Soo Lee, Hyoweon Bang, Mi-Kyung Lee, Soon-Chul Myung, Young Wook Choi, Kap-sok Lee, Seong Jun Seo, Min Won Lee Source Type: journals

Mouse models for melanoma: a personal perspectiveEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Mouse models for melanoma: a personal perspective. Experimental Dermatology 2009.Abstract: Complex biological processes often require in vivo analysis, and many important research advances have been made using mice as a model for the study of various biological systems. Cutaneous melanomas are tumors originating from skin melanocytes, which are present in hair follicles, and interfollicular epidermal and dermal layers. Until recently, mouse melanoma models were largely based on transplantation models, i.e. transplantation of either syngeneic or xenogeneic melanoma cells into wild type or genetica...
Source: Experimental Dermatology - October 21, 2009 Category: Dermatology Authors: Jürgen C. Becker, Roland Houben, David Schrama, Heike Voigt, Selma Ugurel, Ralph A. Reisfeld Source Type: journals

Influence of narrowband UVB phototherapy on vitamin D and folate statusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Low-dose nUVB treatment gives a significant increase (P < 0.001) of the vitamin D status in persons with low initial levels of 25(OH)D, but no effect on the folate level. (Source: Experimental Dermatology)
Source: Experimental Dermatology - October 21, 2009 Category: Dermatology Authors: Emanuela Cicarma, Cato Mørk, Alina Carmen Porojnicu, Asta Juzeniene, Tran Thi Thu Tam, Arne Dahlback, Johan Moan Source Type: journals

Dysregulation of CD36 upon TLR-2 stimulation in monocytes from patients with atopic dermatitis and the TLR2 R753Q polymorphismEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The differences in CD36 expression in TLR-2 polymorph AD patients compared to wild type AD patients and healthy controls may be associated with an enhanced susceptibility to skin infections with S. aureus. (Source: Experimental Dermatology)
Source: Experimental Dermatology - October 21, 2009 Category: Dermatology Authors: Margarete Niebuhr, Jens Langnickel, Stefanie Sigel, Thomas Werfel Source Type: journals

Upregulation of cathepsin S in psoriatic keratinocytesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Upregulation of cathepsin S in psoriatic keratinocytes. Experimental Dermatology 2009.Abstract: Cathepsin S (CATS) is a cysteine protease, well known for its role in MHC class II-mediated antigen presentation and extracellular matrix degradation. Disturbance of the expression or metabolism of this protease is a concomitant feature of several diseases. Given this importance we studied the localization and regulation of CATS expression in normal and pathological human/mouse skin. In normal human skin CATS-immunostaining is mainly present in the dermis and is localized in macrophages, Langerhans, T-...
Source: Experimental Dermatology - October 21, 2009 Category: Dermatology Authors: Alexander Schönefuß, Wiebke Wendt, Benjamin Schattling, Roxane Schulten, Klaus Hoffmann, Markus Stuecker, Christian Tigges, Hermann Lübbert, Christine Stichel Source Type: journals

Lipidome of narrow-band ultraviolet B irradiated keratinocytes shows apoptotic hallmarksEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: NB-UVB affected the cellular lipidome of keratinocytes in strictly apoptosis-specific manner. (Source: Experimental Dermatology)
Source: Experimental Dermatology - October 20, 2009 Category: Dermatology Authors: Adam Reich, Dominik Schwudke, Michael Meurer, Bodo Lehmann, Andrej Shevchenko Source Type: journals

Fluorescence induction of protoporphyrin IX by a new 5-aminolevulinic acid nanoemulsion used for photodynamic therapy in a full-thickness ex vivo skin modelEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Fluorescence induction of protoporphyrin IX by a new 5-aminolevulinic acid nanoemulsion used for photodynamic therapy in a full-thickness ex vivo skin model. Experimental Dermatology 2009.Abstract: An ex vivo porcine skin model was utilized to analyse the penetration of 5-aminolevulinic acid (5-ALA) contained in a nanoemulsion-based formulation BF-200 ALA (10% 5-ALA-hydrochloride) versus 16% aminolevulinate methyl ester-hydrochloride in a commercially cream (MAL cream) by fluorescence microscopy of their common metabolite protoporphyrin IX (PpIX) after 3, 5, 8 and 12 h. Fluorescence signals of Pp...
Source: Experimental Dermatology - October 20, 2009 Category: Dermatology Authors: Tim Maisch, Francesco Santarelli, Stephan Schreml, Philipp Babilas, Rolf-Markus Szeimies Source Type: journals

CorrigendumEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Experimental Dermatology)
Source: Experimental Dermatology - October 20, 2009 Category: Dermatology Source Type: journals

The NF-κB signalling pathway is involved in the LPS/IL-2-induced upregulation of FoxP3 expression in human CD4+CD25high regulatory T cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: The NF-[kappa]B signalling pathway is involved in the LPS/IL-2-induced upregulation of FoxP3 expression in human CD4+CD25high regulatory T cells. Experimental Dermatology 2009.Abstract: Regulatory T cells (Treg) have been found to be central for host defense regulation against microbial antigens, the prevention of allergic and autoimmune diseases and the suppression of effective tumor immune responses. However, the influence of the microenvironment and the mechanisms leading to their activation in the periphery still remain unclear. In vitro infection models revealed that survival and suppressive...
Source: Experimental Dermatology - October 20, 2009 Category: Dermatology Authors: Linda Milkova, Verena Voelcker, Inka Forstreuter, Ulrich Sack, Ulf Anderegg, Jan-C. Simon, Corinna Maier-Simon Source Type: journals

Nestin and SOX9 and SOX10 transcription factors are coexpressed in melanomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the expression levels of nestin, BRN2, SOX9 and SOX10 were analysed in tissues of melanoma (n = 78) and melanocytic nevi (n = 26) by immunohistochemistry. All proteins were highly expressed in primary and metastatic melanomas and, apart from BRN2, showed much lower levels in melanocytic nevi. Significant coexpression of nestin with SOX9 and SOX10 was found in primary melanoma confirming our in vitro data. Correlation analysis with clinicopathological data revealed that nestin was significantly associated with presence of ulceration in primary tumors and SOX9 with more advanced stage of disease. Our data reve...
Source: Experimental Dermatology - October 20, 2009 Category: Dermatology Authors: Renato M. Bakos, Tanja Maier, Robert Besch, Dominik S. Mestel, Thomas Ruzicka, Richard A. Sturm, Carola Berking Source Type: journals

Probing tumor and peritumoral tissues in superficial and nodular basal cell carcinoma using polarized Raman microspectroscopyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study reports the use of polarized Raman microspectroscopy on the nodular and superficial types to discriminate between healthy epidermis and tumor, and between normal and peritumoral stroma. This technique probes additional information than conventional Raman spectroscopy because it is sensitive to the molecular ordering of tissue components. Depolarization ratios and hierarchical cluster analysis demonstrate that polarized Raman microspectroscopy can better identify the tumor and the peritumoral dermis than conventional Raman microspectroscopy, and hence gives potential complementary data about their molecular chara...
Source: Experimental Dermatology - October 20, 2009 Category: Dermatology Authors: Elodie Ly, Anne Durlach, Frank Antonicelli, Philippe Bernard, Michel Manfait, Olivier Piot Source Type: journals

Absence of modulation of CD4+CD25high regulatory T cells in CTCL patients treated with bexaroteneEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Absence of modulation of CD4+CD25high regulatory T cells in CTCL patients treated with bexarotene. Experimental Dermatology 2009.Abstract: Cutaneous T-cell lymphoma (CTCL) are a heterogeneous group of lymphoproliferative disorders, characterized by the infiltration of the epidermis by mature and activated malignant CD4+ T-lymphocytes. Retinoids such as retinoic acid and synthetic analogues have long been used alone or in combination with other therapies for CTCL. Bexarotene, the first synthetic highly selective RXR retinoid, was approved for the treatment of all stages of CTCL in patients refract...
Source: Experimental Dermatology - October 20, 2009 Category: Dermatology Authors: Anne Chantal Knol, Gaëlle Quéreux, Anabelle Brocard, Fabienne Ballanger, Amir Khammari, Jean-Michel Nguyen, Brigitte Dréno Source Type: journals

Effect of honey and its major royal jelly protein 1 on cytokine and MMP-9 mRNA transcripts in human keratinocytesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Effect of honey and its major royal jelly protein 1 on cytokine and MMP-9 mRNA transcripts in human keratinocytes. Experimental Dermatology 2009: 00: 000[ndash]000.Abstract: Honey has been used since ancient times as a remedy in wound healing. However, even though the results from randomized clinical trials document that honey accelerates wound healing, no study dealing with its influence on human skin cells (epidermal keratinocytes and dermal fibroblast) has been performed. We demonstrate that keratinocytes, which are known to be involved in wound healing, are responsible for elevated production...
Source: Experimental Dermatology - October 20, 2009 Category: Dermatology Authors: Juraj Majtan, Pawan Kumar, Tomas Majtan, Andrew F. Walls, Jaroslav Klaudiny Source Type: journals

About the cutaneous targets of bexarotene in CTCL patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: About the cutaneous targets of bexarotene in CTCL patients. Experimental Dermatology 2009.Abstract: There are several approved therapies for cutaneous T-cell lymphoma (CTCL). The retinoids are one of the major biologic response modifiers used in CTCL, producing good response rates but few complete responses. Bexarotene has been demonstrated to act on malignant T-cells by inducing their apoptosis, but nothing is known about its role on keratinocytes and Langerhans cells. Immunohistochemical analysis using CD1a, HLA-DR, ICAM-1 (activation markers), CD95 and CD40 (apoptosis markers) was conducted on...
Source: Experimental Dermatology - October 20, 2009 Category: Dermatology Authors: Anne Chantal Knol, Gaëlle Quéreux, Anabelle Brocard, Fabienne Ballanger, Amir Khammari, Jean-Michel Nguyen, Brigitte Dréno Source Type: journals

The role of keratinocyte growth factor in melanogenesis: a possible mechanism for the initiation of solar lentiginesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: The role of keratinocyte growth factor in melanogenesis: a possible mechanism for the initiation of solar lentigines. Experimental Dermatology 2009.Abstract: Solar lentigines (SLs) are hyperpigmentary lesions presented on sun-exposed areas of the skin and associated with ageing. The molecular mechanism of SL initiation is not completely understood. Ultraviolet B (UVB) stimulates keratinocytes to produce interlukin-1 alpha (IL-1[alpha]), which then induces keratinocyte growth factor (KGF) secretion; therefore, we examined their possible roles in the induction of SLs. We found that KGF increases pi...
Source: Experimental Dermatology - September 23, 2009 Category: Dermatology Authors: Nannan Chen, Yaping Hu, Wen-Hwa Li, Magdalena Eisinger, Miri Seiberg, Connie B. Lin Source Type: journals

Hirseins inhibit melanogenesis by regulating the gene expressions of Mitf and melanogenesis enzymesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report for the first time that HA and HB can down-regulate melanogenesis by down-regulating Mitf gene expression, leading to reduced expressions of Tyr, Trp1 and Dct. The hirseins were also able to reduce the kinase activity, suggesting the possible involvement of PKC in the overall ability of the hirseins to down-regulate melanogenesis. (Source: Experimental Dermatology)
Source: Experimental Dermatology - September 16, 2009 Category: Dermatology Authors: Myra O. Villareal, Junkyu Han, Parida Yamada, Hideyuki Shigemori, Hiroko Isoda Source Type: journals

Large scale study of epidermal recovery after stratum corneum removal: dynamics of genomic responseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Large scale study of epidermal recovery after stratum corneum removal: dynamics of genomic response. Experimental Dermatology 2009.Abstract: The stratum corneum (SC) is a superficial skin compartment that protects the body from the outside environment. Any disturbance of this function induces cascading steps of molecular and cellular repair in the whole epidermis. The aim of this study was to investigate epidermal gene expression following SC removal by tape stripping. Twenty-nine healthy male volunteers were included (27 ± 4 years old). Tape stripping was processed on one inner forearm, the oth...
Source: Experimental Dermatology - September 16, 2009 Category: Dermatology Authors: Peggy Sextius, Claire Marionnet, Francois-Xavier Bon, Albane Lamy de La Chapelle, Charlotte Tacheau, Morad Lahfa, Alain Mauviel, Bruno A. Bernard, Jacques Leclaire, Francoise Bernerd, Louis Dubertret Source Type: journals

Intracellular delivery of major histocompatibility complex class I-binding epitopes: dendritic cells loaded and matured with cationic peptide/poly(I:C) complexes efficiently activate T cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Based on their role for the induction of T-cell responses, dendritic cells (DCs) are popular candidates in cancer vaccine development. We established a novel single-step intracellular delivery of peptide/poly(I:C) complexes for antigen loading and Toll-like receptor-3 (TLR3)-mediated maturation of human DCs using a cell-penetrating peptide (tat49[ndash]57: RKKRRQRRR) as delivery vector. Towards this end, a cationic tat-sequence was fused with an antigenic, major histocompatibility complex (MHC) class I-binding melanoma epitope (Melan-A/Mart-1 sequence: ELAGIGILTV) and then mixed with negatively charged poly(I:C) ...
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Holger A. Haenssle, Petra Riedl, Timo Buhl, Anke Schardt, Albert Rosenberger, Michael P. Schön, Reinhold Schirmbeck Source Type: journals

Topical calcitriol protects from UV-induced genetic damage but suppresses cutaneous immunity in humansEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Calcitriol, the biologically active form of vitamin D, has been reported to cause both suppressive and protective immune effects in mice. Its immune effects in vivo in humans are unclear. We investigated the in vivo effects of topical calcitriol on minimal erythema dose and skin immune responses in healthy volunteers. We found that calcitriol did not protect from ultraviolet (UV)-induced erythema (sunburn) when applied either 24 h before or immediately after irradiation, although it decreased the density of sunburn cells and thymine dimers seen on biopsy when applied 24 h before and again immediately after irradi...
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Diona L. Damian, Young Jin Kim, Katie M. Dixon, Gary M. Halliday, Arash Javeri, Rebecca S. Mason Source Type: journals

Efficacy of quantifying melanosome transfer with flow cytometry in a human melanocyte–HaCaT keratinocyte co-culture system in vitroEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we describe a simple, specific, reproducible and quantitative assay system to assess melanosome transfer. We first established a co-culture model of normal human epidermal melanocytes and HaCaT keratinocytes. The cells were co-cultured for 72 h in a serum-free keratinocyte growth media and double labelled with Fluorescein isothiocyanate (FITC)-conjugated antibody against the melanosome-specific protein gp100, and with Phycoerythrin (PE)-conjugated antibody against the keratinocyte-specific marker cytokeratin. Then, the cells were examined using co-focal microscope and flow cytometry. The increased melanosome...
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Hui-Jun Ma, Guang Zhao, Shao-Xia Zi, Dong-Guang Li, Wen Liu, Qing-Qi Yang Source Type: journals

Activation of toll-like receptors 2, 3 or 5 induces matrix metalloproteinase-1 and -9 expression with the involvement of MAPKs and NF-κB in human epidermal keratinocytesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we demonstrate that the mRNA and protein expressions of MMP-1 and MMP-9 in human epidermal keratinocytes are induced by ligands for TLR2, TLR3 and TLR5 [Pam3CSK4, Poly(I:C) and flagellin, respectively] in a dose-dependent manner. We also found that the ligands for TLR2, TLR3 and TLR5 activate the MAP kinases, JNK and p38 MAPK, but not ERK1/2. Furthermore, treatment with the ligands for TLR2, TLR3 and TLR5 also induced the degradation of I[kappa]B-[alpha] and activated the nuclear translocation of NF-[kappa]B. MMP-1 induction by the ligands for TLR2, TLR3 and TLR5 was inhibited by pretreatment with BAY11-7082...
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Youngae Lee, Hyunjung Kim, Sangmin Kim, Kyu Han Kim, Jin Ho Chung Source Type: journals

In vivo and in vitro evidence for epidermal H2O2-mediated oxidative stress in piebaldismEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as:In vivo and in vitro evidence for epidermal H2O2-mediated oxidative stress in piebaldism. Experimental Dermatology 2009.Abstract: Piebaldism is characterised by the absence of pigment in patches on the skin, usually present at birth. Mutations in the kit gene are documented. Clinically this disorder can mimic vitiligo. Here, we show for the first time the presence of oxidised pteridine-induced fluorescence in association with H2O2-mediated stress in piebald patches employing Wood's light and in vivo FT-Raman spectroscopy. In situ immunofluorescence data revealed low catalase and methionine sulphox...
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Tayyebeh Vafaee, Hartmut Rokos, Mohamed M. A. E. L. Salem, Karin U. Schallreuter Source Type: journals

Long-term efficacy of etanercept in hidradenitis suppurativa: results from an open-label phase II prospective trialEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The first treatment course achieved long-term disease remission in almost one-third of patients. The remaining needed a second treatment course but even in that case, their disease severity at restart was significantly lower compared with baseline. (Source: Experimental Dermatology)
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Aimilia Pelekanou, Theodora Kanni, Athina Savva, Maria Mouktaroudi, Maria Raftogiannis, Antigone Kotsaki, Evangelos J. Giamarellos-Bourboulis Source Type: journals

IL-6 Stimulates but is not essential for stratum corneum formation and permeability barrier development during gestationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, exogenous IL-6 accelerates epidermal ontogenesis, but it is not essential for normal epidermal maturation. (Source: Experimental Dermatology)
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Yan J. Jiang, Biao Lu, Debbie Crumrine, Peter M. Elias, Kenneth R. Feingold Source Type: journals

Human mast cells express androgen receptors but treatment with testosterone exerts no influence on IgE-independent mast cell degranulation elicited by neuromuscular blocking agentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Human mast cells express androgen receptors but treatment with testosterone exerts no influence on IgE-independent mast cell degranulation elicited by neuromuscular blocking agents. Experimental Dermatology 2009; x: xx[ndash]xx.Abstract: Women predominate in the anaphylactic reactions to neuromuscular blocking agents (NMBA). The expression of oestrogen receptors has been demonstrated in mast cells and oestrogen treatment can enhance mast cell degranulation, but the influence of androgens remains largely unclear. Our immunocytochemical study showed the expression of androgen receptor (AR) in mast ...
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: WenChieh Chen, Isabelle Beck, Wolfgang Schober, Knut Brockow, Renate Effner, Jeroen T. M. Buters, Heidrun Behrendt, Johannes Ring Source Type: journals

Increased subcutaneous adipose tissue impairs dermal function in diet-induced obese miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined this issue in HR-1 hairless mice given a high-fat diet (HFD). After having been fed with HFD for 12 weeks, the mice became obese and the subcutaneous adipose tissue layer was significantly thickened, while the dermal layer became significantly thinner than that of control mice fed normal diet. However, the thickness of the dermal layer was not changed in the ear pinna, which lacks a subcutaneous adipose layer, suggesting that increase of subcutaneous adipose tissue may induce dermal changes. The number of dermal fibroblasts in the dermis was significantly reduced in obese mice, although there was no change in g...
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Tomonobu Ezure, Satoshi Amano Source Type: journals

CC chemokines as potential immunologic markers correlated with clinical improvement of atopic dermatitis patients by immunotherapyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: CC chemokines as potential immunologic markers correlated with clinical improvement of atopic dermatitis patients by immunotherapy. Experimental Dermatology 2009.Abstract: Although immunotherapy is not accepted as a curative treatment for atopic dermatitis (AD), most studies have shown positive effects of immunotherapy on AD patients. The serum levels of CC chemokine ligand 17 (CCL17), CCL22 and CCL18 have been reported to be highly correlated with disease severity, which suggests important roles for CC chemokines in the pathogenesis of AD.Objective: The purpose of this study was to investigate t...
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Yeon Sook Kwon, Sang Ho Oh, Wen Hao Wu, Byung Gi Bae, Hee Jung Lee, Min-Geol Lee, Kwang Hoon Lee Source Type: journals

Signal transducer and activator of transcription 3 upregulates interleukin-8 expression at the level of transcription in human melanoma cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Signal transducer and activator of transcription 3 upregulates interleukin-8 expression at the level of transcription in human melanoma cells. Experimental Dermatology 2009.Abstract: Many melanoma cells continuously produce interleukin-8 (IL-8). The involvement of signal transducer and activator of transcription 3 (STAT3) in the constant production of IL-8 in melanoma cells was examined. The level of IL-8 production correlated well with that of the phosphorylated (activated) STAT3 in six human melanoma cell lines. Introduction of the constitutively activated form of STAT3 (STAT3-C) into WM35 mela...
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Masahiro Oka, Masanobu Sakaguchi, Taro Okada, Hiroshi Nagai, Michitaka Ozaki, Toyo Yoshioka, Hiroshi Inoue, Naofumi Mukaida, Ushio Kikkawa, Chikako Nishigori Source Type: journals

Tumor necrosis factor-α processing inhibitor-1 inhibits skin fibrosis in a bleomycin-induced murine model of sclerodermaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we found that sTNFRp55 also is increased in the bleomycin (BLM)-induced murine model of SSc. Therefore, we examined the effect of tumor necrosis factor-[alpha] processing inhibitor-1 (TAPI-1), the inhibitor of TNFRp55 sheddase, in this model. TAPI-1 was administered weekly to mice with skin fibrosis induced by daily BLM injections. TAPI-1 significantly suppressed BLM-induced skin thickness and the number of myofibroblasts. It also inhibited the increase of serum sTNFRp55 after 3 weeks of BLM injections. The mRNA expression of collagen type I [alpha]1, transforming growth factor-[beta]1 and alpha smooth muscl...
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Mika Terao, Hiroyuki Murota, Shun Kitaba, Ichiro Katayama Source Type: journals

Tyrosine kinase 2 and interferon regulatory factor 5 polymorphisms are associated with discoid and subacute cutaneous lupus erythematosusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Tyrosine kinase 2 and interferon regulatory factor 5 polymorphisms are associated with discoid and subacute cutaneous lupus erythematosus. Experimental Dermatology 2009.Abstract: Lupus erythematosus (LE) is a heterogeneous disease ranging from skin-restricted manifestations to a progressive multisystem disease. The specific skin lesions include chronic cutaneous, subacute cutaneous and acute cutaneous LE. Both genetic and environmental factors are involved in the development of LE. However, reports on the genetic background of cutaneous lupus erythematosus (CLE) forms, namely discoid (DLE) and su...
Source: Experimental Dermatology - September 15, 2009 Category: Dermatology Authors: Tiina M. Järvinen, Anna Hellquist, Sari Koskenmies, Elisabet Einarsdottir, Lotta L. E. Koskinen, Leila Jeskanen, Linda Berglind, Jaana Panelius, Taina Hasan, Annamari Ranki, Juha Kere, Ulpu Saarialho-Kere Source Type: journals

Serum levels of IL-18 and sIL-2R in patients with alopecia areata receiving combined therapy with oral cyclosporine and steroidsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, increased serum sIL-2R level and lower IL-18 level at baseline was associated with a poor prognosis in patients with AA. (Source: Experimental Dermatology)
Source: Experimental Dermatology - September 14, 2009 Category: Dermatology Authors: Deborah Lee, Soon-Kwon Hong, Sung-Wook Park, Dae-Young Hur, Ji-Hong Shon, Jae-Gook Shin, Seon-Wook Hwang, Ho-Suk Sung Source Type: journals

Application of stereology to dermatological researchEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Application of stereology to dermatological research. Experimental Dermatology 2009.Abstract: Stereology is a set of mathematical and statistical tools to estimate three-dimensional (3-D) characteristics of objects from regular two-dimensional (2-D) sections. In medicine and biology, it can be used to estimate features such as cell volume, cell membrane surface area, total length of blood vessels per volume tissue and total number of cells. The unbiased quantification of these 3-D features allows for a better understanding of morphology in vivo compared with 2-D methods. This review provides an i...
Source: Experimental Dermatology - September 14, 2009 Category: Dermatology Authors: Søren Kamp, Gregor Borut Ernst Jemec, Kåre Kemp, Cecilia Rosada Kjeldsen, Karin Stenderup, Bente Pakkenberg, Tomas Norman Dam Source Type: journals

Enhanced T-cell activation by immature dendritic cells loaded with HSP70-expressing heat-killed melanoma cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, HSP70-expressing melanoma cells could be generated by heat-killing. Loading iDCs with heat-killed melanoma cells resulted in a superior priming of autologous T cells in vitro. (Source: Experimental Dermatology)
Source: Experimental Dermatology - September 14, 2009 Category: Dermatology Authors: Susanne Knudsen, Anke Schardt, Timo Buhl, Lars Boeckmann, Michael P. Schön, Christine Neumann, Holger A. Haenssle Source Type: journals

The potential role of c-Jun activation in patients with cutaneous lichen planusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: The potential role of c-Jun activation in patients with cutaneous lichen planus. Experimental Dermatology 2009.Abstract: c-Jun, a component of the activating protein-1 transcription factor family, has been known to play an important role in the control of cell proliferation. It is also suspected to be a critical mediator of tumor promotion. However, investigations of c-Jun activation patterns in inflammatory and inflammatory transforming skin diseases have not been described so far. In this work, we show the c-Jun activation pattern in skin samples of patients with cutaneous lichen planus (LP), s...
Source: Experimental Dermatology - September 14, 2009 Category: Dermatology Authors: Zuzana Marčeková, Michael J. Flaig, Maja Keku[scaron], Thomas Ruzicka, Rudolf A. Rupec Source Type: journals

Isolation of human eosinophils: microbead method has no impact on IL-5 sustained viabilityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Multi-antibody eosinophil isolation represents a substantial advantage over anti-CD-16 microbeads when isolating large numbers of eosinophils from concentrated leucocyte preparations. No differential survival was observed. While appropriate consideration of methods is always crucial, multi-antibody eosinophil isolation should not be abandoned completely. (Source: Experimental Dermatology)
Source: Experimental Dermatology - September 14, 2009 Category: Dermatology Authors: Caroline M. Percopo, Kimberly D. Dyer, Kristin E. Killoran, Helene F. Rosenberg Source Type: journals

Fetuin-A promotes primary keratinocyte migration: independent of epidermal growth factor receptor signallingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we found that in human newborn foreskin, a high level of fetuin-A protein is detected throughout the dermis. However, in adult skin a low level of fetuin-A is observed throughout the epidermal and dermal layers, except at regions surrounding hair follicles and at the epidermal-dermal junction where the level of fetuin-A is relatively high. Fetuin-A significantly induces actin-rich protrusions in human primary keratinocytes. Interestingly, blockade of epidermal growth factor (EGF) receptor signalling has a limited effect on fetuin-A promoted 'wound closure' on primary human keratinocytes, but significantly in...
Source: Experimental Dermatology - September 14, 2009 Category: Dermatology Authors: Xue-Qing Wang, Betsy S. Hung, Margit Kempf, Pei-Yun Liu, Andrew J. Dalley, Nicholas A. Saunders, Roy M. Kimble Source Type: journals

Comparative in situ topoproteome analysis reveals differences in patch test-induced eczema: cytotoxicity-dominated nickel versus pleiotrope pollen reactionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Comparative in situ topoproteome analysis reveals differences in patch test-induced eczema: cytotoxicity-dominated nickel versus pleiotrope pollen reaction. Experimental Dermatology 2009.Abstract: A subgroup of patients with atopic eczema develops acute eczematous reactions to type I allergy-inducing agents such as pollen that clinically resemble type IV allergies induced by haptens like metal ions. To clarify the underlying immunologic mechanisms, this study was designed to map the inflammatory in situ topoproteome of eczematous responses to grass/birch pollen and nickel by using atopy patch tes...
Source: Experimental Dermatology - September 14, 2009 Category: Dermatology Authors: Kilian Eyerich, Raik Böckelmann, Ansgar J. Pommer, Stefanie Foerster, Henning Hofmeister, Johannes Huss-Marp, Andrea Cavani, Heidrun Behrendt, Johannes Ring, Harald Gollnick, Bernd Bonnekoh, Claudia Traidl-Hoffmann Source Type: journals

ErratumEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Experimental Dermatology)
Source: Experimental Dermatology - September 14, 2009 Category: Dermatology Source Type: journals

Comment on: Tattooing of skin results in transportation and light-induced decomposition of tattoo pigmentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Comment on: Tattooing of skin results in transportation and light-induced decomposition of tattoo pigments. Experimental Dermatology 2009.Abstract: Decorative tattoos have become a common feature of many societies. Their popularity appears mainly driven by fashion, and scant attention has been paid to any associated risk. The risks can be associated either with the tattooees' proclivity for risk taking in general, or with the substances used in the tattoos. It is well established that tattoo pigments wander widely in the body after they have been injected, and research now suggests that azo pigme...
Source: Experimental Dermatology - September 8, 2009 Category: Dermatology Authors: Gregor B. E. Jemec Source Type: journals

Efficient and selective tumor cell lysis and induction of apoptosis in melanoma cells by a conditional replication-competent CD95L adenovirusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Efficient and selective tumor cell lysis and induction of apoptosis in melanoma cells by a conditional replication-competent CD95L adenovirus. Experimental Dermatology 2009.Abstract: The high mortality of melanoma demands the development of new strategies, and gene therapy may be considered provided improvements in efficacy and selectivity. Overexpression of the death ligand CD95L/FasL has been shown in previous studies as highly effective for apoptosis induction in melanoma cells. For efficient and selective targeting of melanoma, a conditional replication-competent adenoviral vector was constru...
Source: Experimental Dermatology - September 2, 2009 Category: Dermatology Authors: Lothar F. Fecker, Magdalena Schmude, Stefanie Jost, Amir M. Hossini, Almudena Hurtado Picó, Xiaomin Wang, Constanze Schwarz, Henry Fechner, Jürgen Eberle Source Type: journals

Methods in hair research: how to objectively distinguish between anagen and catagen in human hair follicle organ cultureEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Please cite this paper as: Methods in hair research: how to objectively distinguish between anagen and catagen in human hair follicle organ culture. Experimental Dermatology 2009.Abstract: The organ culture of human scalp hair follicles (HFs) is the best currently available assay for hair research in the human system. In order to determine the hair growth-modulatory effects of agents in this assay, one critical read-out parameter is the assessment of whether the test agent has prolonged anagen duration or induced catagen in vitro. However, objective criteria to distinguish between anagen VI HFs and early catagen in human H...
Source: Experimental Dermatology - August 31, 2009 Category: Dermatology Authors: Jennifer Elisabeth Kloepper, Koji Sugawara, Yusur Al-Nuaimi, Erzsébet Gáspár, Nina van Beek, Ralf Paus Source Type: journals