Experimental HematologyThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

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Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Experimental Hematology)
Source: Experimental Hematology - November 17, 2009 Category: Hematology Source Type: journals

HOZOTs, novel human regulatory T-cell lines, exhibit helper or suppressor activities depending on dendritic cell or anti-CD3 stimulationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: We demonstrated a new function of HOZOTs as helper T cells in addition to suppressor and cytotoxic activities, characterizing HOZOTs as multifunctional T cells. (Source: Experimental Hematology)
Source: Experimental Hematology - October 12, 2009 Category: Hematology Authors: Akira Sugimoto, Motoyuki Suzuki, Takeshi Otani, Ayumi Okochi, Makoto Takeuchi, Fumiyuki Yamasaki, Shuji Nakamura, Masayoshi Kibata Tags: Immunobiology Source Type: journals

Aldehyde dehydrogenase activity among primary leukemia cells is associated with stem cell features and correlates with adverse clinical outcomesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Objective: Animal models have provided evidence for the existence of leukemia stem cells (LSC). However, prospective isolation of human LSC from patients with acute myeloid leukemia (AML), as well as the assessment of their clinical significance, has remained a major challenge.Materials and Methods: We have studied the functional characteristics of a subset of leukemia cells that expressed CD34 and high aldehyde dehydrogenase activity (ALDHbr), which was freshly isolated from the mononuclear cells at the time of diagnosis from the marrow of 68 consecutive patients suffering from AML.Results: The percentage of ALDHbr cells ...
Source: Experimental Hematology - October 12, 2009 Category: Hematology Authors: Dan Ran, Mario Schubert, Larissa Pietsch, Isabel Taubert, Patrick Wuchter, Volker Eckstein, Thomas Bruckner, Margot Zoeller, Anthony D. Ho Tags: Hematological Malignancies Source Type: journals

Polycythemia vera erythroid precursors exhibit increased proliferation and apoptosis resistance associated with abnormal RAS and PI3K pathway activationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Objective: Polycythemia vera (PV) is characterized by erythrocytosis associated with the presence of the activating JAK2V617F mutation in a variable proportion of hematopoietic cells. JAK2V617F is detected in other myeloproliferative neoplasms, does not appear to be the PV-initiating event, and its specific role in deregulated erythropoiesis in PV is incompletely understood. We investigated the pathogenesis of PV to characterize abnormal proliferation and apoptosis responses and aberrant oncogenic pathway activation in primary PV erythroid precursors.Materials and Methods: Peripheral blood CD34+ cells isolated from PV pati...
Source: Experimental Hematology - October 7, 2009 Category: Hematology Authors: Jacob P. Laubach, Ping Fu, Xiaohong Jiang, Kelly H. Salter, Anil Potti, Murat O. Arcasoy Tags: Hematological Malignancies Source Type: journals

Hematopoietic stem cell origin of human fibroblasts: Cell culture studies of female recipients of gender-mismatched stem cell transplantation and patients with chronic myelogenous leukemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: These studies strongly suggest that human fibroblasts are derived from HSCs. In addition, the results suggest that fibrosis seen in patients with CML may be a part of the clonal process. (Source: Experimental Hematology)
Source: Experimental Hematology - September 28, 2009 Category: Hematology Authors: Keisuke Shirai, Yasuhiko Sera, William Bulkeley, Meenal Mehrotra, Omar Moussa, Amanda C. LaRue, Dennis K. Watson, Robert K. Stuart, John Lazarchick, Makio Ogawa Tags: Stem Cell Plasticity Source Type: journals

Dasatinib inhibits the secretion of TNF-α following TLR stimulation in vitro and in vivoEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: These findings further support the ability of dasatinib to modulate the host immune response and highlights scope for off-target applications of dasatinib for the control of TNF-α–mediated inflammatory disorders. (Source: Experimental Hematology)
Source: Experimental Hematology - September 28, 2009 Category: Hematology Authors: Cara K. Fraser, Erin L. Lousberg, Raman Kumar, Timothy P. Hughes, Kerrilyn R. Diener, John D. Hayball Tags: Immunobiology Source Type: journals

Bone marrow engraftment but limited expansion of hematopoietic cells from multipotent germline stem cells derived from neonatal mouse testisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: mGS cells produced multipotent hematopoietic progenitor cells with myeloid and lymphoid lineage potential in vitro and localized in the BM after intra-BM injection but, like ES cells, failed to expand or show stem cell repopulating ability in vivo. (Source: Experimental Hematology)
Source: Experimental Hematology - September 25, 2009 Category: Hematology Authors: Momoko Yoshimoto, Toshio Heike, Hsi Chang, Mito Kanatsu-Shinohara, Shiro Baba, Joseph T. Varnau, Takashi Shinohara, Mervin C. Yoder, Tatsutoshi Nakahata Tags: General Hematopoiesis Source Type: journals

Mesenchymal stem cell–educated macrophages: A novel type of alternatively activated macrophagesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We describe a novel type of human macrophage generated in vitro after coculture with MSCs that assumes an immunophenotype defined as IL-10–high, IL-12–low, IL-6–high, and TNF-α–low secreting cells. These MSC-educated macrophages may be a unique and novel type of alternatively activated macrophage with a potentially significant role in tissue repair. (Source: Experimental Hematology)
Source: Experimental Hematology - September 23, 2009 Category: Hematology Authors: Jaehyup Kim, Peiman Hematti Tags: Immunobiology Source Type: journals

Downregulation of GATA-2 and overexpression of adipogenic gene-PPARγ in mesenchymal stem cells from patients with aplastic anemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Aplastic anemia (AA) is characterized by a reduced number of hematopoietic stem cells and fatty replacement in the bone marrow. Transcriptional factor GATA-2 plays several important roles in both hematopoiesis and adipogenesis. Decreased levels of GATA-2 compromise the proliferation and survival of hematopoietic stem cells. GATA-2 suppresses adipocyte differentiation through direct inhibition of adipogenic factors, including peroxisome proliferator-activated receptor−γ (PPARγ). Previous studies have shown that expression of GATA-2 is decreased in marrow CD34-positive cells in AA. To elucidate the mechanisms of fatty ma...
Source: Experimental Hematology - September 23, 2009 Category: Hematology Authors: Yinyan Xu, Yoshiyuki Takahashi, Yue Wang, Asahito Hama, Nobuhiro Nishio, Hideki Muramatsu, Makito Tanaka, Nao Yoshida, Itzel Bustos Villalobos, Hiroshi Yagasaki, Seiji Kojima Tags: General Hematopoiesis Source Type: journals

Inducible transgenes under the control of the hCD68 promoter identifies mouse macrophages with a distribution that differs from the F4/80 - and CSF-1R–expressing populationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Our results show that the hCD68 promoter directs gene expression in a macrophage population distinct from that defined by classical monocyte-macrophage markers or promoters. Whether this population is functionally distinct remains to be established. (Source: Experimental Hematology)
Source: Experimental Hematology - September 23, 2009 Category: Hematology Authors: Manoj M. Pillai, Brian Hayes, Beverly Torok-Storb Tags: General Hematopoiesis Source Type: journals

A novel zebrafish jak2aV581F model shared features of human JAK2V617F polycythemia veraEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we generated a zebrafish ortholog of human JAK2V617F (referred herewith jak2aV581F) by site-directed mutagenesis and examined its relevance as a model of human PV.Materials and Methods: Zebrafish embryos at one-cell stage were injected with jak2aV581F mRNA (200pg/embryo). In some experiments, the embryos were treated with a specific JAK2 inhibitor, TG101209. The effects of jak2a stimulation on hematopoiesis, jak/stat signaling, and erythropoietin signaling were evaluated at 18-somites.Results: Injection with jak2aV581F mRNA significantly increased erythropoiesis, as enumerated by flow cytometry based on gfp+...
Source: Experimental Hematology - September 23, 2009 Category: Hematology Authors: Alvin C.H. Ma, August Fan, Alister C. Ward, Clifford Liongue, Rowena S. Lewis, Suk H. Cheng, P.K. Chan, Sze-Fai Yip, Raymond Liang, Anskar Y.H. Leung Tags: General Hematopoiesis Source Type: journals

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(Source: Experimental Hematology)
Source: Experimental Hematology - September 18, 2009 Category: Hematology Source Type: journals

Accelerated cellular senescence in myelodysplastic syndromeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: These results of our present study suggested an accelerated cellular senescence occurred in MDS, and the cellular senescence may be involved in the progression and prognosis of MDS. (Source: Experimental Hematology)
Source: Experimental Hematology - September 13, 2009 Category: Hematology Authors: Yuan-yuan Wang, Jian-nong Cen, Jun He, Hong-jie Shen, Dan-dan Liu, Yao Li, Xiao-fei Qi, Zi-xing Chen Tags: Hematological Malignancies Source Type: journals

Ex vivo expansion of human hematopoietic stem cells by a small-molecule agonist of c-MPLEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This is the first time that a small-molecule c-MPL agonist has been demonstrated to promote net expansion of HSCs. NR-101 is more efficient in ex vivo expansion of HSCs than TPO. NR-101 could be a useful tool for the therapeutic manipulation of human HSCs. (Source: Experimental Hematology)
Source: Experimental Hematology - September 8, 2009 Category: Hematology Authors: Taito Nishino, Katsuaki Miyaji, Norihisa Ishiwata, Kazutaka Arai, Makiko Yui, Yasuyuki Asai, Hiromitsu Nakauchi, Atsushi Iwama Tags: Stem Cell Biology Source Type: journals

Antiplatelet antibodies in WASP(−) mice correlate with evidence of increased in vivo platelet consumptionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: These findings suggest that platelet WASP deficiency results in an increase in platelet clearance rates by two mechanisms: an antibody-independent mechanism that largely requires WASP deficiency in trans, and an antibody-dependent mechanism that does not. Both an increased incidence of antiplatelet antibodies and an increased susceptibility to their effects contribute to antibody-dependent clearance of WASP(−) platelets. (Source: Experimental Hematology)
Source: Experimental Hematology - September 3, 2009 Category: Hematology Authors: Bindumadhav M. Marathe, Amanda Prislovsky, Alexander Astrakhan, David J. Rawlings, Jim Y. Wan, Ted S. Strom Tags: Megakaryocytopoiesis Source Type: journals

Neurotrophin signaling through tropomyosin receptor kinases contributes to survival and proliferation of non-Hodgkin lymphomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Thus, Trk receptors represent a novel therapeutic target for the treatment of NHL. (Source: Experimental Hematology)
Source: Experimental Hematology - August 31, 2009 Category: Hematology Authors: Lynn F. Sniderhan, Tatiana M. Garcia-Bates, Michael Burgart, Steven H. Bernstein, Richard P. Phipps, Sanjay B. Maggirwar Tags: Hematological Malignancies Source Type: journals

Metabolic fingerprinting of fresh lymphoma samples used to discriminate between follicular and diffuse large B-cell lymphomasEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We have proved that it is possible to differentiate between FL and DLBCL based on their NMR metabolic profiles. This approach may potentially be applicable as a noninvasive tool for diagnostic and treatment follow-up in the clinical setting using conventional magnetic resonance systems. (Source: Experimental Hematology)
Source: Experimental Hematology - August 31, 2009 Category: Hematology Authors: Ignasi Barba, Carolina Sanz, Angels Barbera, Gustavo Tapia, José-Luis Mate, David Garcia-Dorado, Josep-Maria Ribera, Albert Oriol Tags: Hematological Malignancies Source Type: journals

Mechanistic studies on the effects of nicotinamide on megakaryocytic polyploidization and the roles of NAD+ levels and SIRT inhibitionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: NIC greatly increases Mk ploidy under a wide range of conditions without altering Mk morphology. Inhibition of SIRT1 and/or SIRT2 is primarily responsible for NIC effects on Mk maturation. (Source: Experimental Hematology)
Source: Experimental Hematology - August 26, 2009 Category: Hematology Authors: Lisa M. Giammona, Swapna Panuganti, Jan M. Kemper, Pani A. Apostolidis, Stephan Lindsey, Eleftherios T. Papoutsakis, William M. Miller Tags: Megakaryocytopoiesis Source Type: journals

Development of monoclonal antibodies for analyzing immune and hematopoietic systems of common marmosetEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Marmoset immune system was basically similar to human and mouse systems. (Source: Experimental Hematology)
Source: Experimental Hematology - August 26, 2009 Category: Hematology Authors: Yoshie Kametani, Daisuke Suzuki, Kazuyoshi Kohu, Masanobu Satake, Hiroshi Suemizu, Erika Sasaki, Toshio Ito, Norikazu Tamaoki, Tomoko Mizushima, Manabu Ozawa, Kenzaburo Tani, Mitsuaki Kito, Hideo Arai, Akemi Koyanagi, Hideo Yagita, Sonoko Habu Tags: Immunobiology Source Type: journals

Pim-1 expression and monoclonal antibody targeting in human leukemia cell linesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Pim-1 is variably expressed in leukemia cell lines and associated with drug resistance. Targeting Pim-1 with monoclonal antibody could be explored for the treatment of leukemia and may represent a novel strategy to overcome drug resistance. (Source: Experimental Hematology)
Source: Experimental Hematology - August 24, 2009 Category: Hematology Authors: Jie Li, Xiu Feng Hu, Bruce E. Loveland, Pei Xiang Xing Tags: Hematological Malignancies Source Type: journals

Activation of SHIP via a small molecule agonist kills multiple myeloma cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: These results provide the basis for the further study of small molecule SHIP activators to improve MM patient outcomes. (Source: Experimental Hematology)
Source: Experimental Hematology - August 24, 2009 Category: Hematology Authors: Michael Kennah, Tien Yin Yau, Matt Nodwell, Gerald Krystal, Raymond J. Andersen, Christopher J. Ong, Alice L-F Mui Tags: Hematological Malignancies Source Type: journals

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(Source: Experimental Hematology)
Source: Experimental Hematology - August 13, 2009 Category: Hematology Source Type: journals

Characterization and transplantation of induced megakaryocytes from hematopoietic stem cells for rapid platelet recovery by a two-step serum-free procedureEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: In conclusion, we have developed a serum-free megakaryocyte induction medium, and the combination of serum-free megakaryocyte and serum-free hematopoietic stem cell media can generate a large amount of functional megakaryocytes efficiently. Our method represents a promising source of megakaryocytes and platelets for future cell therapy. (Source: Experimental Hematology)
Source: Experimental Hematology - August 5, 2009 Category: Hematology Authors: Te-Wei Chen, Shiaw-Min Hwang, I-Ming Chu, Shu-Ching Hsu, Tzu-Bou Hsieh, Chao-Ling Yao Tags: Megakaryocytopoiesis Source Type: journals

Efficacious but insidious: A retrospective analysis of fludarabine-induced myelotoxicity using long-term culture–initiating cells in 100 follicular lymphoma patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Fludarabine and procarbazine have a dramatic influence, especially on the most immature hematopoietic cells, mirrored in reduced numbers of LTC-IC. This finding is consistent with clinical observations (poor mobilization after fludarabine) and offers an insight into the mechanism of fludarabine-induced myelotoxicity. (Source: Experimental Hematology)
Source: Experimental Hematology - August 2, 2009 Category: Hematology Authors: Andrea Janikova, Zdenek Koristek, Jaroslava Vinklarkova, Tomas Pavlik, Michal Sticha, Milan Navratil, Zdenek Kral, Ingrid Vasova, Jiri Mayer Tags: Hematological Malignancies Source Type: journals

Differential gene expression signatures of adult peripheral blood vs cord blood monocyte-derived immature and mature dendritic cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Taken together, these results suggest that specific genetic signatures might be responsible for differential DC differentiation and maturation between APB vs CB, and may provide insight into molecular mechanisms regulating differential immune responses between neonates and adults. (Source: Experimental Hematology)
Source: Experimental Hematology - August 2, 2009 Category: Hematology Authors: Hong Jiang, Carmella van de Ven, Laxmi Baxi, Prakash Satwani, Mitchell S. Cairo Tags: Immunobiology Source Type: journals

Cotransplantation with MSCs improves engraftment of HSCs after autologous intra-bone marrow transplantation in nonhuman primatesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Cotransplantation with MSCs would improve the efficacy of transplantation of gene-modified HSCs in primates, with enhanced engraftment in BM as well as increased chimerism in peripheral blood through migration and homing. (Source: Experimental Hematology)
Source: Experimental Hematology - July 26, 2009 Category: Hematology Authors: Shigeo Masuda, Naohide Ageyama, Hiroaki Shibata, Yoko Obara, Tamako Ikeda, Kengo Takeuchi, Yasuji Ueda, Keiya Ozawa, Yutaka Hanazono Tags: Stem Cell Transplantation Source Type: journals

Characterization of natural killer and natural killer–like T cells derived from ex vivo expanded and activated cord blood mononuclear cells: Implications for adoptive cellular immunotherapyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We reported the ability to ex vivo expand (EvE) CB mononuclear cells (MNC) after short-term incubation with anti-CD3, interleukin (IL)-2, IL-7, and IL-12 (antibody/cytokine [AB/CY]) into subpopulations of CD3−/56+ natural killer (NK) cells with enhanced in vitro and in vivo tumor cytotoxicity.Materials and Methods: We compared 2- vs 7-day EvE of rethawed CB MNCs in AB/CY and activation of NK and NK-like T (NKT) cell (CD3+/56+) subsets expressing specific NK-cell receptors along with IL-15, IL-18, and interferon-γ production.Results: Nonadherent total cell number were significantly increased at day 7 (p (Source: Experimental Hematology)
Source: Experimental Hematology - July 26, 2009 Category: Hematology Authors: Janet Ayello, Carmella van de Ven, Evan Cairo, Jessica Hochberg, Laxmi Baxi, Prakash Satwani, Mitchell S. Cairo Tags: Immunotherapy Source Type: journals

Comparative analyses of the small molecule thrombopoietin receptor agonist eltrombopag and thrombopoietin on in vitro platelet functionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: These results demonstrate that the nonpeptidyl TPOR agonist eltrombopag stimulates platelet signal transduction with little or no effect on overall platelet function, in contrast to TPO, which significantly primes platelet activation. These data demonstrate that effects of TPOR ligands on platelet function can vary depending on the specific mechanism utilized to stimulate the TPOR. (Source: Experimental Hematology)
Source: Experimental Hematology - July 23, 2009 Category: Hematology Authors: Joseph A. Erhardt, Connie L. Erickson-Miller, Manuel Aivado, Melanie Abboud, Kodandaram Pillarisetti, John R. Toomey Tags: General Hematopoiesis Source Type: journals

Critical role for ERK1/2 in bone marrow and fetal liver–derived primary megakaryocyte differentiation, motility, and proplatelet formationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This report demonstrates a critical role of MEK-ERK1/2 pathway in MK differentiation, motility, and proplatelet formation. This study highlights several differences between BM- and FL-derived MKs, which are discussed. (Source: Experimental Hematology)
Source: Experimental Hematology - July 20, 2009 Category: Hematology Authors: Alexandra Mazharian, Steve P. Watson, Sonia Séverin Tags: Megakaryocytopoiesis Source Type: journals

JAK2V617F allele burden discriminates essential thrombocythemia from a subset of prefibrotic-stage primary myelofibrosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, histopathological classification of 490 MPN cases was correlated with the allelic burden of JAK2V617F and MPLW515L.Results: Ph− MPN entities largely overlap with regard to JAK2V617F and MPLW515L allele burden, but ET displayed mutant allele burden 40% JAK2V617F alleles (median, 24% JAK2V617F alleles; n=90; p (Source: Experimental Hematology)
Source: Experimental Hematology - July 20, 2009 Category: Hematology Authors: Kais Hussein, Oliver Bock, Katharina Theophile, Nils von Neuhoff, Thomas Buhr, Jerome Schlué, Guntram Büsche, Hans Kreipe Tags: Hematological Malignancies Source Type: journals

Transmission and expansion of HOXB4-induced leukemia in two immunosuppressed dogs: Implications for a new canine leukemia modelEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The engraftment and expansion of these leukemia cells in dogs will allow studies into the biology of leukemia and development and evaluation of novel antileukemia therapies in a clinically relevant large animal model. (Source: Experimental Hematology)
Source: Experimental Hematology - July 20, 2009 Category: Hematology Authors: Monica S. Thakar, Xiao-Bing Zhang, Brian C. Beard, George E. Sale, Erlinda B. Santos, Laura Peterson, Hans-Peter Kiem, Brenda M. Sandmaier Tags: Gene Therapy Source Type: journals

Clonal analyses define the relationships between chromosomal abnormalities and JAK2V617F in patients with Ph-negative myeloproliferative neoplasms∗Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This study indicates that MPN hematopoietic progenitor cells do not necessarily always acquire genetic events in the same sequence. (Chromosomally abnormal progenitor cells are closely associated with JAK2V617F homozygosity; p=0.0001.). Chromosomal abnormalities such as +8, +9 can occasionally precede acquisition of JAK2V617F. These findings support the existence of earlier genetic events that precede JAK2V617F or cytogenetic abnormalities in MPN hematopoietic progenitor cells. (Source: Experimental Hematology)
Source: Experimental Hematology - July 16, 2009 Category: Hematology Authors: Xiaoli Wang, Amanda LeBlanc, Steven Gruenstein, Mingjiang Xu, John Mascarenhas, Brenda Panzera, Nathaniel Wisch, Charles Parker, Judith D. Goldberg, Josef Prchal, Ronald Hoffman, Vesna Najfeld Tags: Hematological Malignancies Source Type: journals

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(Source: Experimental Hematology)
Source: Experimental Hematology - July 16, 2009 Category: Hematology Source Type: journals

Mechanistic insight into WEB-2170-induced apoptosis in human acute myelogenous leukemia cells: The crucial role of PTENEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: We demonstrated that WEB-2170 is a powerful antileukemic agent with interesting translational opportunities to treat AML and described mechanisms of drug-induced intrinsic and extrinsic apoptosis both in AML cell lines and blasts from AML patients by addressing PTEN as the master regulator of the whole process. (Source: Experimental Hematology)
Source: Experimental Hematology - July 15, 2009 Category: Hematology Authors: Cristina Cellai, Anna Laurenzana, Elisa Bianchi, Sara Sdelci, Rossella Manfredini, Alessandro M. Vannucchi, Roberto Caporale, Manjola Balliu, Francesco Mannelli, Sergio Ferrari, Alberto Bosi, Debora Miniati, Pier L. Cocco, Steeve Veronneau, Jana Stankova, Tags: Hematological Malignancies Source Type: journals

Dynamics of α-globin locus chromatin structure and gene expression during erythroid differentiation of human CD34+ cells in cultureEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Our results indicate that remodeling of the upstream elements may be the primary event in activation of α-globin gene expression. Activation of α-globin genes upon EPO treatment involves initial binding of Pol II, downregulation of pre-existing factors like NF-E2, removal of CTCF from the locus, then rebinding of CTCF in an altered pattern, and concurrent or subsequent binding of transcription factors like GATA-1. (Source: Experimental Hematology)
Source: Experimental Hematology - July 14, 2009 Category: Hematology Authors: Milind C. Mahajan, Subhradip Karmakar, Peter E. Newburger, Diane S. Krause, Sherman M. Weissman Tags: Erythropoiesis Source Type: journals

Analysis of NK cell/DC interaction in NK-type lymphoproliferative disease of granular lymphocytes (LDGL): role of DNAM-1 and NKp30Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Besides expanding knowledge of the molecular basis of the NK/DC cross-talk, our study demonstrates that NK cells from NK-LDGL patients are impaired in their ability to interact with Mo-DC. The possible relationship between such abnormal NK cell/DC interactions and chronic NK cell proliferation are discussed. (Source: Experimental Hematology)
Source: Experimental Hematology - July 5, 2009 Category: Hematology Authors: Mirna Balsamo, Renato Zambello, Antonella Teramo, Marco Pedrazzi, Bianca Sparatore, Francesca Scordamaglia, Daniela Pende, Maria Cristina Mingari, Lorenzo Moretta, Alessandro Moretta, Gianpietro Semenzato, Massimo Vitale Tags: Hematological Malignancies Source Type: journals

Stimulated stromal cells induce γ-globin gene expression in erythroid cells via nitric oxide productionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: These results demonstrate an arrangement by which NO and fetal hemoglobin inducers may stimulate globin genes in erythroid cells via the common paracrine effect of bone marrow stromal cells. (Source: Experimental Hematology)
Source: Experimental Hematology - July 2, 2009 Category: Hematology Authors: Vladan P. Čokić, Bojana B. Beleslin-Čokić, Reginald D. Smith, Antaeus P. Economou, Larry M. Wahl, Constance T. Noguchi, Alan N. Schechter Tags: Microenvironment Source Type: journals

Transcriptional activation of the γ-globin gene in baboons treated with decitabine and in cultured erythroid progenitor cells involves different mechanismsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The mechanism responsible for increased γ-globin expression in cultured EPC was unexpectedly not associated with increased DNA hypomethylation of the γ-globin gene promoter compared to normal BM erythroid cells, in contrast to BM erythroid cells of decitabine-treated baboons. Rather, increased fetal hemoglobin in EPC cultures was associated with a fetal Iγ/Vγ chain ratio and a difference in the size of the BCL11A protein compared to normal BM erythroid cells. (Source: Experimental Hematology)
Source: Experimental Hematology - July 2, 2009 Category: Hematology Authors: Janet Chin, Mahipal Singh, Virryan Banzon, Kestis Vaitkus, Vinzon Ibanez, Tatiana Kouznetsova, Nadim Mahmud, Joseph DeSimone, Donald Lavelle Tags: Erythropoiesis Source Type: journals

Hematopoietic stem cell origin of adipocytesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Together, these studies clarify the current controversy regarding the ability of HSCs to give rise to adipocytes. Furthermore, our primary culture method that generates adipocytes from uncommitted hematopoietic cells should contribute to the studies of the mechanisms of early adipocytic differentiation and may lead to development of therapeutic solutions for many general obesity issues. (Source: Experimental Hematology)
Source: Experimental Hematology - July 2, 2009 Category: Hematology Authors: Yasuhiko Sera, Amanda C. LaRue, Omar Moussa, Meenal Mehrotra, James D. Duncan, Christopher R. Williams, Eishi Nishimoto, Bradley A. Schulte, Patricia M. Watson, Dennis K. Watson, Makio Ogawa Tags: Stem Cell Plasticity Source Type: journals

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(Source: Experimental Hematology)
Source: Experimental Hematology - June 25, 2009 Category: Hematology Source Type: journals

JAK2V617F allele burden and thrombosis: A direct comparison in essential thrombocythemia and polycythemia veraEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: These findings support the concept of a continuum between ET JAK2 mutated and PV, not only in reference to the hematological phenotype, but also in terms of vascular events. (Source: Experimental Hematology)
Source: Experimental Hematology - June 24, 2009 Category: Hematology Authors: Alessandra Carobbio, Guido Finazzi, Elisabetta Antonioli, Paola Guglielmelli, Alessandro M. Vannucchi, Chiara M. Dellacasa, Silvia Salmoiraghi, Federica Delaini, Alessandro Rambaldi, Tiziano Barbui Tags: Clinical Investigation Source Type: journals

A proof of concept study: Human C48-placenta immunoregulatory factor is an effective, single therapeutic agent enabling allogeneic, nonmanipulated murine bone marrow transplantationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Findings of this study provide the proof of concept that C48—a novel, single, bifunctional therapeutic modality enabled successful allogeneic, unmanipulated bone marrow transplantation without GVHD, and with lasting specific tolerance. (Source: Experimental Hematology)
Source: Experimental Hematology - June 17, 2009 Category: Hematology Authors: Chaya Moroz, Leonid Traub, Esther Rabizadeh, Muayad A. Zahalka Tags: Stem Cell Transplantation Source Type: journals

Identification of small Sca-1+, Lin−, CD45− multipotential cells in the neonatal murine retinaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The neonatal retina is an abundant postnatal source of multipotential VSEL-like cells that can differentiate in culture into a variety of lineages. (Source: Experimental Hematology)
Source: Experimental Hematology - June 17, 2009 Category: Hematology Authors: Yongqing Liu, Ling Gao, Ewa K. Zuba-Surma, Xiaoyan Peng, Magdalena Kucia, Mariusz Z. Ratajczak, Wei Wang, Volker Enzman, Henry J. Kaplan, Douglas C. Dean Tags: Stem Cell Plasticity Source Type: journals

Glycosaminoglycan mimetics–induced mobilization of hematopoietic progenitors and stem cells into mouse peripheral blood: Structure/function insights∗Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This study demonstrates that GAG mimetics induce efficient mobilization of HSPCs, associated with an activation of pro−MMP-9 and a modification in the SDF-1 concentration gradient between bone marrow and peripheral blood. We suggest that structural features of GAGs can modify the nature of mobilized cells. (Source: Experimental Hematology)
Source: Experimental Hematology - June 17, 2009 Category: Hematology Authors: Patricia Albanese, Danielle Caruelle, Guilhem Frescaline, Jean Delbé, Laurence Petit-Cocault, Eric Huet, Nathalie Charnaux, Georges Uzan, Dulce Papy-Garcia, José Courty Tags: Stem Cell Biology Source Type: journals

Splenic stromal niches support hematopoiesis of dendritic-like cells from precursors in bone marrow and spleenEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The outcome has been development of a coculture system that supports hematopoiesis of immature myeloid dendritic-like cells in vitro. Although production of monocytes can occur in the presence of stroma-conditioned medium alone, production of DC is dependent on stromal cell interaction. Results presented here raise questions about the role of spleen as a site for DC hematopoiesis from endogenous precursors. (Source: Experimental Hematology)
Source: Experimental Hematology - June 17, 2009 Category: Hematology Authors: Pravin Periasamy, Jonathan K.H. Tan, Kristin L. Griffiths, Helen C. O'Neill Tags: General Hematopoiesis Source Type: journals

Elevated endothelial progenitor cells during painful sickle cell crisisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Objective: Circulating endothelial progenitor cells (EPCs) counts were determined in patients with sickle cell disease (SCD) to elucidate their role in SCD-related ischemia-induced angiogenesis and reendothelialization.Materials and Methods: Circulating EPC counts (KDR+/CD34+/Cd45dim cells) and their relation to serum levels of EPC mobilizing growth factors erythropoietin, vascular endothelial growth factor, and interleukin-8 were investigated in SCD patients during asymptomatic state (n=66) and painful crisis (n=36) and compared to healthy controls (n=13).Results: EPC counts were comparable between controls (0; range, 0�...
Source: Experimental Hematology - June 17, 2009 Category: Hematology Authors: Rachel T. van Beem, Erfan Nur, Jaap Jan Zwaginga, Precious P. Landburg, Eduard J. van Beers, Ashley J. Duits, Dees P. Brandjes, Ingrid Lommerse, Hetty C. de Boer, C. Ellen van der Schoot, John-John B. Schnog, Bart J. Biemond, CURAMA Study Group Tags: General Hematopoiesis Source Type: journals

Polymorphisms in Toll-like receptor genes and susceptibility to infections in allogeneic stem cell transplantationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Thus, fungal colonization may not predict susceptibility to infection in the presence of these single nucleotide polymorphisms. The finding that defective viral but not fungal sensing may predict susceptibility to infection highlights the divergent function of TLRs in the pathogenesis of opportunistic infections. (Source: Experimental Hematology)
Source: Experimental Hematology - June 17, 2009 Category: Hematology Authors: Agostinho Carvalho, Cristina Cunha, Alessandra Carotti, Teresa Aloisi, Ornella Guarrera, Mauro Di Ianni, Franca Falzetti, Francesco Bistoni, Franco Aversa, Lucia Pitzurra, Fernando Rodrigues, Luigina Romani Tags: Clinical Investigation Source Type: journals

Nanon effects on mammalian cell long-term cultures: A caveat for experimental hematologists dealing with in vitro long-term culture assaysEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This letter aims to make cell culture investigators using human or animal long-term cell cultures aware of potential contamination by nanobacteria-like particles , recently proposed as nanons . (Source: Experimental Hematology)
Source: Experimental Hematology - June 14, 2009 Category: Hematology Authors: Peppino Mirabelli, Francesca D'Alessio, Elisabetta Mariotti, Maria Romano, Giuliana Fortunato, Marica Gemei, Rosa Di Noto, Luigi Del Vecchio Tags: Letter to the Editor Source Type: journals

Cord blood stem cell expansion is permissive to epigenetic regulation and environmental cuesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Our studies indicate there are interactions between intrinsic factors influenced by epigenetic mechanisms and external environmental signals in the regulation of HSC expansion. Epigenetic influences on HSC can be accentuated by environmental factors. Regulation of the rate of divisions may be a critical determinant for the maintenance of HSC functional potency during ex vivo expansion. (Source: Experimental Hematology)
Source: Experimental Hematology - June 7, 2009 Category: Hematology Authors: Hiroto Araki, Sudhakar Baluchamy, Kazumi Yoshinaga, Benjamin Petro, Sakina Petiwala, Ritesh Parajuli, Mohammed Milhem, Donald Lavelle, Joseph DeSimone, Nadim Mahmud Tags: Stem Cell Biology Source Type: journals