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Oxidative Damage: The Biochemical Mechanism of Cellular Injury and Necrosis in Choline Deficiency.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Oxidative stress and damage are characterized by decreased tissue antioxidant levels, consumption of tissue alpha-tocopherol and increased lipid peroxidation. These processes occur earlier than necrosis in the liver, heart, kidney and brain of weanling rats fed a choline deficient (CD) diet. In tissues, water-soluble antioxidants were analyzed as total reactive antioxidant potential (TRAP), alpha-tocopherol content was estimated from homogenate chemiluminescence (homogenate-CL), and lipid peroxidation was evaluated by thiobarbituric acid reactive substances (TBARS). Histopathology showed hepatic steatosis at days 1-7, ...
Source: Experimental and Molecular Pathology - November 11, 2009 Category: Pathology Authors: Repetto MG, Ossani G, Monserrat AJ, Boveris A Tags: Exp Mol Pathol Source Type: journals

The study of ISO induced heart failure rat model.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSION: 85/340 mg/kg ISO induced HF rat model was superior to the other two dose ISO induced HF rat models. Pro-inflammatory cytokines might contribute to HF and myocardial fibrosis through promotion of MMPs expression in cardiac fibroblasts. PMID: 19909738 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - November 10, 2009 Category: Pathology Authors: Feng W, Li W Tags: Exp Mol Pathol Source Type: journals

Epigenetic modifications induced by RGC-32 in colon cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
First described as a cell cycle activator, RGC-32 is both an activator and a substrate for CDC2. Deregulation of RGC-32 expression has been detected in a wide variety of human cancers. We have now shown that RGC-32 is expressed in precancerous states, and its expression is significantly higher in adenomas than in normal colon tissue. The expression of RGC-32 was higher in advanced stages of colon cancer than in precancerous states or the initial stages of colon cancer. In order to identify the genes that are regulated by RGC-32, we used gene array analysis to investigate the effect of RGC-32 knockdown on gene expressio...
Source: Experimental and Molecular Pathology - October 30, 2009 Category: Pathology Authors: Vlaicu SI, Tegla CA, Cudrici CD, Fosbrink M, Nguyen V, Azimzadeh P, Rus V, Chen H, Mircea PA, Shamsuddin A, Rus H Tags: Exp Mol Pathol Source Type: journals

Correlation of CpG island methylator phenotype with poor prognosis in hepatocellular carcinoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the promoter methylation status of ten genes in 60 cases of hepatocellular carcinoma (HCC), 60 cases of paired non-tumor tissues, and 6 cases of normal tissues by methylation-specific PCR. The average methylated-gene numbers were significantly different between HCC and nontumor tissues (p < 0.001). We found metastasis, gamma-glutamyl transpeptidase (GGT) and tumor node metastasis (TNM) stage were significantly different among patients with different CIMP status. Patients with high frequency CIMP tumors had significantly worse survival than patients with intermediate frequency or no CIMP tumors (p < 0.01 a...
Source: Experimental and Molecular Pathology - October 28, 2009 Category: Pathology Authors: Cheng Y, Zhang C, Zhao J, Wang C, Xu Y, Han Z, Jiang G, Guo X, Li R, Bu X, Wu M, Wei L Tags: Exp Mol Pathol Source Type: journals

Cytokine-dependent Activation of Small Hepatocyte-like Progenitor Cells in Retrorsine-induced Rat Liver Injury.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the possibility that SHPCs require cytokine priming prior to becoming growth factor responsive in this model of liver injury and regeneration. Male Fischer 344 rats were treated with retrorsine (30 mg/kg ip) at 6 and 8 weeks of age. Retrorsine-exposed and age-matched control rats were randomized into dexamethasone-treated and no DEX groups. DEX-treated animals were either given a single dose of DEX (2 mg/kg ip) at the time of PH or multiple DEX treatments (2 mg/kg ip each) at 24h and 1h before PH and 1, 2, and 3 days post-PH. A subset of rats received ten micrograms of recombinant IL6 protein, administered intr...
Source: Experimental and Molecular Pathology - October 26, 2009 Category: Pathology Authors: Best DH, Butz GM, Coleman WB Tags: Exp Mol Pathol Source Type: journals

The relationship between transforming growth factor beta1 expression and cold ischemia injury of rat donor kidney.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion : There was the significant correlation between the expression of TGF-beta1 and the degree of cold ischemia injury. The results suggest that TGF-beta1 might play the key role in regeneration and reparation of renal tubular epithelial cell injury. The overexpression of TGF-beta1 might be one of the mechanisms that initiate chronic allograft nephropathy. PMID: 19874817 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 26, 2009 Category: Pathology Authors: He W, Liu K, Ding H Tags: Exp Mol Pathol Source Type: journals

Disruption of actin cytoskeleton mediates loss of tensile stress induced early phenotypic modulation of vascular smooth muscle cells in organ culture.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study suggests that disrupting actin cytoskeleton integrity may contribute to the pathogenesis of vascular diseases. PMID: 19874818 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 26, 2009 Category: Pathology Authors: Zheng JP, Ju D, Shen J, Yang M, Li L Tags: Exp Mol Pathol Source Type: journals

mRNA and microRNA Expression Analysis in Laser Capture Microdissected-Prostate Biopsies: Valuable tool for risk assessment and prevention trials.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this study was to determine the feasibility of mRNA and miRNA expression analysis in laser-capture microdissected (LCM) formalin-fixed paraffin-embedded archived prostate biopsies compared to the gold standard of frozen tissue. We analyzed the expression of compartment-specific and PCa-related genes in epithelial and stromal tissues collected from paired sets of archived prostate biopsies and frozen radical prostatectomy specimens from three patients. Our results showed appropriate compartment-specific and PC-related expression with good within patient agreement between the FFPE-biopsies and the frozen tis...
Source: Experimental and Molecular Pathology - October 26, 2009 Category: Pathology Authors: Nonn L, Vaishnav A, Gallagher L, Gann PH Tags: Exp Mol Pathol Source Type: journals

A Validation Study of a New Molecular Diagnostic Assay: The Dartmouth-Hitchcock Medical Center Experience with the GeneSearch BLN Assay in Breast Sentinel Lymph Nodes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Discussion: The sensitivity of the assay sampling from the outer node tissue was high (88.9%) and identical to that validated in the large registration study in which half of the node was assessed as alternate slices (87.6%). Our protocol uses this assay as an adjunct to traditional histologic evaluation, to reduce and standardize the number of tissue sections needed for thorough SLN evaluation, to enhance our ability to bank RNA. PMID: 19840784 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 15, 2009 Category: Pathology Authors: Tafe LJ, Schwab MC, Lefferts JA, Wells WA, Tsongalis GJ Tags: Exp Mol Pathol Source Type: journals

Optimisation of Postmortem Tissue Preservation and Alternative Protocol for Serotonin Transporter Gene Polymorphisms Amplification in Sids and Siud Cases.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we compared the quality of DNA and RNA extracted from tissues differently preserved. As expected, the DNA and RNA from formalin-fixed and paraffin-embedded tissues, formalin-acetic acid-alcohol tissues and ethanol tissues were of poor quality and not adequate for subsequent molecular analysis. The best results were obtained with RNAlater preserved tissues: this buffer was equivalet, if not superior, to freezing method for preservation of postmortem DNA and RNA. In addition, we introduce a new protocol for the amplification of the serotonin transporter gene promoter region (5-HTT) ideal to obtain the increase ...
Source: Experimental and Molecular Pathology - October 14, 2009 Category: Pathology Authors: Casale V, Oneda R, Lavezzi AM, Matturri L Tags: Exp Mol Pathol Source Type: journals

Oxidative stress induced by HIV-1 F34IVpr in Schizosaccharomyces pombe is one of its multiple functions.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, a mutant derivative of Vpr (F34IVpr), which causes transitent G(2) arrest with little or not effect of cell killing, was used to study the molecular impact of Vpr on cellular oxidative stress responses in Sch. pombe. We demonstrated here that F34IVpr triggers low level of complex and atypical oxidative stress responses in comparison with its parental strain SP223 in early (14-h) and late (35-h) log phase cultures. Specifically, F34IVpr production in Sch. pombe causes significantly elevated levels of reactive oxygen species such as superoxide and peroxides; meanwhile, it also induces decreased levels of gluta...
Source: Experimental and Molecular Pathology - October 14, 2009 Category: Pathology Authors: Stromájer-Rácz T, Gazdag Z, Belágyi J, Vágvölgyi C, Zhao RY, Pesti M Tags: Exp Mol Pathol Source Type: journals

Association of PPARalpha gene polymorphisms and lipid serum levels in a Brazilian elderly population.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Peroxisome proliferator-activated receptor alpha (PPARalpha) is a nuclear transcription factor strictly involved in lipid and lipoprotein metabolisms. Thus, PPARalpha gene polymorphisms have been investigated as cardiovascular risk factors. We aimed to investigate associations of L162V and intron 7G>C polymorphisms with common morbidities affecting a Brazilian elderly cohort as well as with lipid and protein serum levels. Genotyping was performed by polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) and allele frequencies were determined. In addition, we performed the linkage d...
Source: Experimental and Molecular Pathology - October 8, 2009 Category: Pathology Authors: Chen ES, Mazzotti DR, Furuya TK, Cendoroglo MS, Ramos LR, Araujo LQ, Burbano RR, Cardoso Smith MD Tags: Exp Mol Pathol Source Type: journals

PIK3CA Mutations mostly Begin to Develop in Ductal Carcinoma of the Breast.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Somatic mutations of PIK3CA are found in 20% to 40% of invasive breast cancers. To investigate the frequency of PIK3CA mutations in the intraductal proliferative lesions of the breast, which are precursor lesions for invasive carcinoma, we analyzed 125 intraductal proliferative lesions and 108 invasive breast cancer tissues for PIK3CA mutations in this study. Target cells were precisely isolated using a laser capture microdissection (LCM) system. Genomic DNA was extracted with QIAmp DNA Micro Kit. PCR amplification was done for exons 9 and 20 of PIK3CA, where 90% of mutations clustered, and the products were directly s...
Source: Experimental and Molecular Pathology - October 6, 2009 Category: Pathology Authors: Li H, Zhu R, Wang L, Zhu T, Li Q, Chen Q, Wang H, Zhu H Tags: Exp Mol Pathol Source Type: journals

Quantitative DNA Methylation Analysis of Laser Capture Microdissected Formalin-fixed and Paraffin-embedded Tissues.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We developed an assay to quantify DNA methylation in breast cancer cells isolated by laser capture microdissection (LCM). The assay uses methylation sensitive restriction enzyme (MSRE) digestion and quantitative polymerase chain reaction (qPCR). To assess the validity and precision of the assay, we prepared standard samples with expected methylation percentage (MP) for two gene promoters (PLAU (plasminogen inhibitor, urokinase) and TIMP3 (TIMP metallopeptidase inhibitor 3)) that we compared with measured MPs. We found good linearity of MSRE digestion and qPCR procedures for both promoters (beta = 0.90-1.19 +/- 0.05-0.1...
Source: Experimental and Molecular Pathology - October 6, 2009 Category: Pathology Authors: Gagnon JF, Sanschagrin F, Jacob S, Tremblay AA, Provencher L, Robert J, Morin C, Diorio C Tags: Exp Mol Pathol Source Type: journals

Minichromosome maintenance protein 3 is a candidate proliferation marker in papillary thyroid carcinoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The proliferative capacity of tumor cells is a characteristic feature in the whole growing tumors. Many pathologists and clinicians have used the estimation of cell proliferation for prognostic information. Minichromosome maintenance protein 3 (MCM3) is known to have a role on the initiation and regulation of DNA replication during cell cycle. The aim of this study is to evaluate the potential applicability of one of the MCM proteins, MCM3, as a proliferation marker in papillary thyroid carcinoma (PTC) with correlation to clinicopathologic parameters. We performed the immunohistochemical analysis for MCM3 and Ki-67 in ...
Source: Experimental and Molecular Pathology - October 6, 2009 Category: Pathology Authors: Lee YS, Ha SA, Kim HJ, Shin SM, Kim HK, Kim S, Kang CS, Lee KY, Hong OK, Lee SH, Kwon HS, Cha BY, Kim JW Tags: Exp Mol Pathol Source Type: journals

Prostate carcinogenesis induced by N-Methyl-Nnitrosourea (MNU) in gerbils: Histopathological diagnosis and potential invasiveness mediated by extracellular matrix components.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study prostate lesions were induced in gerbils (Meriones unguiculatus) treated with a single N-Methyl-N-Nitrosourea (MNU) dose; thus, the incidence, latency and histology of these lesions were evaluated. Fibrillar elements of the extracellular matrix associated with microinvasive sites were also investigated. Animals were divided into 5 groups, including 2 control groups: 1) remained untreated; 2) received the corn oil vehicle (vehicle, 0.1 ml/application) and three different tumor induction regimens: 1) received MNU (30mg/Kg) and weekly testosterone (2mg/Kg) (MNU+testosterone); 2) received only MNU (30m...
Source: Experimental and Molecular Pathology - October 6, 2009 Category: Pathology Authors: Gonçalves BF, Zanetoni C, Scarano WR, Góes RM, Vilamaior PS, Taboga SR, Campos SG Tags: Exp Mol Pathol Source Type: journals

GDF-5 is suppressed by IL-1beta and enhances TGFbeta3mediated chondrogenic differentiation in human rheumatoid fibroblastlike synoviocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions. GDF-5 is expressed in FLS-RA and HC-RA, and its expression is strongly downregulated by proinflammatory cytokines. MEK-ERK pathway is a negative regulator of GDF-5 expression in FLS-RA. In FLS-RA, synergy between GDF-5 and TGF-beta(3) enhances chondrogenesis. Anti-inflammatory drugs combined with GDF5 might be a new therapeutic treatment for RA. PMID: 19818765 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 6, 2009 Category: Pathology Authors: Liu FL, Lin LH, Sytwu HK, Chang DM Tags: Exp Mol Pathol Source Type: journals

Establishment of shared-antigen reactive cytotoxic T lymphocyte using co-stimulatory molecule introduced autologous cancer cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cytotoxic T lymphocytes (CTLs) plays essential role in immunological responses for tumor rejection. In the past decade, many tumor-associated antigens (TAAs) have been identified predominantly in melanomas. Several clinical trials based on such antigenic peptides with or without adjuvants brought about partially favorable results, suggesting that identification of more immunogenic TAAs is needed. We show here the successful establishment of human leukocyte antigen (HLA)-A24-restricted CTL (TcLHK2 line1) from a pleural effusion of lung cancer patient, using B7.1 (CD80) transduced autologous lung cancer cells as an antig...
Source: Experimental and Molecular Pathology - October 6, 2009 Category: Pathology Authors: Hirohashi Y, Torigoe T, Hirai I, Tamura Y, Nakatsugawa M, Inoue Y, Kanaseki T, Kamiguchi K, Ikeda H, Sasaki A, Yamanaka N, Sato N Tags: Exp Mol Pathol Source Type: journals

CFTR and Tight Junctions in Cultured Bronchial Epithelial Cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Airway epithelial salt and water transport takes place through paracellular and transcellular pathways. This transport depends critically on the epithelial sodium channel (ENaC) and the cystic fibrosis transmembrane conductance regulator (CFTR), operating in concert with the paracellular pathway through the tight junctions (TJ). Normal (16HBE14o-), cystic fibrosis (CFBE41o-), and corrected CFBE41o- (CFBE41o- pCep4 overexpressing wtCFTR) airway epithelial cell lines were cultured under isotonic conditions. Transepithelial electrical resistance (TEER) was measured as indicator of the tightness of the cultures. Morphology...
Source: Experimental and Molecular Pathology - October 6, 2009 Category: Pathology Authors: Nilsson HE, Dragomir A, Lazorova L, Johannesson M, Roomans GM Tags: Exp Mol Pathol Source Type: journals

Concordance of DNA Methylation pattern in Plasma and Tumor DNA of Egyptian Hepatocellular Carcinoma Patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19818350 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 5, 2009 Category: Pathology Authors: Iyer P, Zekri AR, Hung CW, Schiefelbein E, Ismail K, Hablas A, Seifeldin IA, Soliman AS Tags: Exp Mol Pathol Source Type: journals

Interpreting aCGH-defined karyotypic changes in gliomas using copy number status, loss of heterozygosity and allelic ratios.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We have used SNP mapping arrays to simultaneously record copy number changes, loss of heterozygosity and allele ratios (ploidy) in a series of 13 gliomas. This combined analysis has defined novel amplification events in this tumor type involving chr1:241544532-243005121 and chr18:54716681-54917277 which contain the AKT3 and ZNF532 genes respectively. The high resolution of this analysis has also identified homozygous deletions involving chr17:25600031-26490848 and Chr19:53883612-55061878. Throughout the karyotypes of these tumors, the combined analysis revealed counter intuitive relationships between copy number and LO...
Source: Experimental and Molecular Pathology - October 5, 2009 Category: Pathology Authors: Cowell JK, Lo KC, Luce J, Hawthorn L Tags: Exp Mol Pathol Source Type: journals

Blocking Platelet/Endothelial Cell Adhesion Molecule 1 (PECAM) Inhibits Disease Progression and Prevents Joint Erosion in Established Collagen Antibody Induced Arthritis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report that treatment of symptomatic mice with a PECAM-Fc chimera significantly reduced inflammation and virtually eliminated cartilage and bone destruction. The results suggest that therapies that block PECAM function may be beneficial in the treatment of established arthritis. PMID: 19800878 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 30, 2009 Category: Pathology Authors: Dasgupta B, Chew T, Deroche A, Muller WA Tags: Exp Mol Pathol Source Type: journals

High glucose enhances Angiotensin II mediated peroxisome proliferation activated receptor-gamma inactivation in human coronary artery endothelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Activation of the renin-angiotensin system plays an important role in the pathogenesis of vascular complications of hyperglycemia. Clinical studies have demonstrated that hypoglycemic effects of peroxisome proliferation-activated receptor-gamma (PPAR-gamma) activation is potentially associated with a significant decrease of cardiovascular disease events in diabetes patients. We assessed the effect of high glucose on the Angiotensin II (Ang II)-induced the inactivation of PPAR-gamma and its signal pathways in human coronary artery endothelial cells (HCAECs). The expression of angiotensin II receptor I (AT1R) protein was...
Source: Experimental and Molecular Pathology - September 27, 2009 Category: Pathology Authors: Min Q, Bai YT, Jia G, Wu J, Xiang JZ Tags: Exp Mol Pathol Source Type: journals

Comparison of different tissue sampling methods for protein extraction from formalin-fixed and paraffin-embedded tissue specimens.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The sampling of FFPE specimens for protein extraction and analysis can be standardized, uncovering equal amounts of tissue and protein. In addition, the proteins extracted from FFPE tissue seem to be the same compared with those extracted from fresh frozen tissue. PMID: 19782068 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 22, 2009 Category: Pathology Authors: Gräntzdörffer I, Yumlu S, Gioeva Z, von Wasielewski R, Ebert MP, Röcken C Tags: Exp Mol Pathol Source Type: journals

RhoB enhances migration and MMP1 expression of prostate cancer DU145.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rho family protein regulates variety of cellular functions as cytoskeletal organization, cell proliferation and apoptosis. In the present study, we demonstrate that RhoB over-expressed prostate cancer cells showed an enhanced cell motility and the administration of the GSK-3 inhibitors inhibited this increase in migration. Among the extracellular matrix and adhesion related molecules, MMP1 RNA expression was increased in RhoB over-expressed cells, administration of MMP inhibitor suppressed the collagen gel invasion in these cells. This is the first report evaluating RhoB function and the downstream signaling events in ...
Source: Experimental and Molecular Pathology - September 22, 2009 Category: Pathology Authors: Yoneda M, Hirokawa YS, Ohashi A, Uchida K, Kami D, Watanabe M, Yokoi T, Shiraishi T, Wakusawa S Tags: Exp Mol Pathol Source Type: journals

Marrow stromal fibroblastic cell cultivation in vitro on decellularized bone marrow extracellular matrix.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The in vitro biocompatibility of decellularized bone marrow extracellular matrix was evaluated. Following a freeze-thaw cycle, sectioned discs of fresh frozen rat metaphyseal bone were sequentially incubated in solutions of hypertonic, then hypotonic Ringer's solution, followed by deoxycholic acid, then DNAase I. The adequacy of decellularization of marrow stroma was examined by light microscopy. Marrow stromal fibroblastic cells were harvested by dispersion of rat long bone marrow, followed by concentration by discontinuous Ficoll-Paque gradient centrifugation. The fibroblastic cells were expanded by in vitro cultivat...
Source: Experimental and Molecular Pathology - September 20, 2009 Category: Pathology Authors: Dutra TF, French SW Tags: Exp Mol Pathol Source Type: journals

Inactivation of LGI1 expression accompanies early stage hyperplasia of prostate epithelium in the TRAMP murine model of prostate cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The LGI1 gene has been implicated in tumor cell invasion through regulation of the ERK pathway. To determine whether human prostate cancer cells (PC3, 22RV, Du145) are similarly affected by exposure to LGI1, we conducted scratch wound assays and demonstrated that the secreted LGI1 protein can reduce cell motility, an essential component of invasion and metastasis. These studies have now been extended to an in vivo mouse model of prostate cancer. Using a BAC transgenic mouse expressing a GFP reporter gene under the control of cis regulatory elements, we demonstrated that LGI1 is highly expressed in the normal prostate e...
Source: Experimental and Molecular Pathology - September 20, 2009 Category: Pathology Authors: Cowell JK, Head K, Kunapuli P, Vaughan M, Karasik E, Foster B Tags: Exp Mol Pathol Source Type: journals

Toll-like receptor and chemokine receptor expression in HIV infected T-lymphocyte subsets.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present investigation, flow cytometric techniques were utilized to evaluate 100 cases of HIV and 20 normal controls for CXCR4, CCR5, and TLR4 expression in CD4 positive T cells, CD8 positive T cells, regulatory T cells, and T(h)17 cells, and fluorescence intensity values were determined. TLR4 was expressed by CD4+ T cells and CD8+ T cells in 97 cases, by regulatory T cells in 88 of 95 cases, and by Th17 cells in 93 of 95 cases, while it remained negative in all 20 normal controls. These data indicate that TLR4 upregulation is not limited to gram-negative bacterial infection nor is expression limited to myeloid c...
Source: Experimental and Molecular Pathology - September 17, 2009 Category: Pathology Authors: Sanders CM, Cruse JM, Lewis RE Tags: Exp Mol Pathol Source Type: journals

Association between vascular endothelial growth factor gene polymorphisms and age-related macular degeneration in a Polish population.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The pathogenesis of age-related macular degeneration (AMD) is thought to be determined by an array of environmental and genetic factors. The association of increased expression of vascular endothelial growth factor (VEGF) with AMD, especially the wet form of AMD, was reported in several studies. The VEGF gene is highly polymorphic and some of its polymorphisms may affect its expression. In our work we searched for an association between the -460C>T (rs833061) and -634G>C (rs2010963) polymorphisms of the VEGF gene and the occurrence of AMD and its dry and wet forms. We have chosen these polymorphisms because they ...
Source: Experimental and Molecular Pathology - September 13, 2009 Category: Pathology Authors: Janik-Papis K, Zaras M, Krzyzanowska A, Wozniak K, Blasiak J, Szaflik J, Szaflik JP Tags: Exp Mol Pathol Source Type: journals

Hemostatic properties of a venomic protein in rat organ trauma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Previous in vitro work characterized the protease Q8009 isolated from the venom of the Australian brown snake Pseudonaja textilis textilis with Factor Xa-like activity and hemostatic properties. The purpose of the work described here characterizes the in vivo hemostatic properties in a rat model of parenchymatous organ injury. The key parameters of activity included reduction in time-to-hemostasis and total volume of blood loss in spleen, liver and kidney wound models in rats. The surgical protocols involved exposure of the organs via a midline abdominal laparotomy. Using a clean metal template with 6, 6.5 9 mm holes f...
Source: Experimental and Molecular Pathology - September 8, 2009 Category: Pathology Authors: Warner RL, McClintock SD, Barron AG, de la Iglesia FA Tags: Exp Mol Pathol Source Type: journals

TGF-beta Induced Fibroblast Activation Protein Expression, Fibroblast Activation Protein Expression Increases the Proliferation, Adhesion, and Migration of HO-8910PM.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study also exams FAP's function in vitro by assaying the proliferation, migration and invasion of ovarian cancer cell line HO-8910PM. PMID: 19747910 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 8, 2009 Category: Pathology Authors: Chen H, Yang WW, Wen QT, Xu L, Chen M Tags: Exp Mol Pathol Source Type: journals

Thrombospondin-1 (TSP-1) Up-regulates Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) Production in Human Tumor Cells: Exploring the Functional Significance in Tumor Cell Invasion.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Thrombospondin-1 (TSP-1), a matrix-bound adhesive glycoprotein, has been shown to modulate tumor progression. We previously demonstrated that TSP-1 up-regulates matrix metalloproteinases MMP-2 and MMP-9. Our studies suggested that the balance between MMPs and tissue inhibitors of metalloproteinases (TIMPs) is a key determinant in tumor cell invasion. We now report that TSP-1 up-regulates TIMP-1 expression in both human breast and prostate cancer cell lines. The effect of TSP-1 on TIMP-1 expression was examined in human breast adenocarcinoma cell lines (MDA-MB-231) and human prostate cancer cell lines (PC3-NI and PC3-ML...
Source: Experimental and Molecular Pathology - September 7, 2009 Category: Pathology Authors: John AS, Hu X, Rothman VL, Tuszynski GP Tags: Exp Mol Pathol Source Type: journals

Heparin Cofactor II in Atherosclerotic Lesions from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) Study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, using samples of coronary arteries obtained from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) study, we explore the local relationship between HCII and (pro)thrombin in atherosclerosis. We found that HCII and (pro)thrombin are co-localized in the lipid-rich necrotic core of atheromas. A significant positive correlation between each protein and the severity of the atherosclerotic lesion was present. These results suggest that HCII is in a position to inhibit thrombin in atherosclerotic lesions where thrombin can exert a proatherogenic inflammatory response. However, these results should...
Source: Experimental and Molecular Pathology - September 7, 2009 Category: Pathology Authors: Rau JC, Deans C, Hoffman MR, Thomas DB, Malcom GT, Zieske AW, Strong JP, Koch GG, Church FC Tags: Exp Mol Pathol Source Type: journals

Molecular Biomarkers for Autoimmune Retinopathies: Significance of Anti-Transducin-Alpha Autoantibodies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, clinical phenotype of patients with anti-transducin-alpha autoantibodies differed from other phenotypes of AR. These patients, often women at a ratio ~2:1, had defects in rod (scotopic) photoreceptor function and typically did not have cancers, whereas anti-recoverin phenotype is associated with cancer and severe loss of rod and cones function, and anti-enolase retinopathy typically presents with cone dysfunction and is equal in cancer and non-cancer patients. Our studies suggest that anti-transducin autoantibodies can serve as molecular biomarkers for retinal phenotypes and could be used for progression of ...
Source: Experimental and Molecular Pathology - September 6, 2009 Category: Pathology Authors: Adamus G, Brown L, Weleber RG Tags: Exp Mol Pathol Source Type: journals

Platelet linoleic acid is a potential biomarker of advanced non-small cell lung cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
New parameters that could be used as tumour markers for lung cancer would be valuable. Our aim was to analyse the fatty acid profiles of total lipids from erythrocytes and platelets from patients with advanced non small cell lung cancer (NSCLC), chronic obstructive pulmonary disease (COPD) and asthma to reveal the fatty acids that could be use as NSCLC biomarkers. 50, 15 and 15 patients with advanced NSCLC, COPD and asthma, and 50 healthy subjects were enrolled in our study. Fatty acid profiles were investigated using gas chromatography/mass spectrometry followed by ROC (Receiver Operating Characteristics) curves analy...
Source: Experimental and Molecular Pathology - September 1, 2009 Category: Pathology Authors: de Castro J, Rodríguez MC, Martínez-Zorzano VS, Llanillo M, Sánchez-Yagüe J Tags: Exp Mol Pathol Source Type: journals

Expression of matrix metalloproteinase 2 and 9 in human trophoblasts of normal and preeclamptic placentas: preliminary findings.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: These preliminary data demonstrate the expression of metalloproteinases 2 and 9 proteins in the placentas of uncomplicated pregnancies. The absence/reduced expression of metalloproteinases 9 in the preeclamptic placentas may be related to insufficient invasion of trophoblast leading to superficial and unsuccessful placentation. PMID: 19716817 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - August 26, 2009 Category: Pathology Authors: Shokry M, Omran OM, Hassan HI, Elsedfy GO, Hussein MR Tags: Exp Mol Pathol Source Type: journals

Expression and prognostic role of Spy1 as a novel cell cycle protein in hepatocellular carcinoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Our results suggested that Spy1 overexpression is involved in the pathogenesis of hepatocellular carcinoma, it may be a favorable independent poor prognostic parameter for hepatocellular carcinoma. PMID: 19686732 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - August 13, 2009 Category: Pathology Authors: Ke Q, Ji J, Cheng C, Zhang Y, Lu M, Wang Y, Zhang L, Li P, Cui X, He S, Shen A Tags: Exp Mol Pathol Source Type: journals

Classic kaposi sarcoma with sarcoid-like granulomas: A case report and literature review.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study presents a case of classic Kaposi sarcoma that contained sarcoid-like granulomas, with a literature review. A soft tissue lesion of the foot was excised from a 74-year-old male. Histopathological examination showed that the tumour tissue consisted of spindle cells, areas of atypical vascular proliferation, and extravasated erythrocytes surrounded by non-caseating granulomas. The patient had no clinical or laboratory findings of sarcoidosis. The case was interpreted as "Kaposi sarcoma containing sarcoid-like granulomas". The association of soft tissue sarcomas with a granulomatous reaction is very rare. A granulo...
Source: Experimental and Molecular Pathology - August 9, 2009 Category: Pathology Authors: Kandemir NO, Yurdakan G, Bektas S, Tekin NS Tags: Exp Mol Pathol Source Type: journals

Hematopoietic stem cell engraftment by early-stage in utero transplantation in a mouse model.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A novel intrauterine transplantation (IUT) approach was developed to improve the efficiency of engraftment of hematopoietic stem cells (HSCs). HSCs with a green fluorescent protein (GFP) reporter gene were transplanted on day 12.5, 13.5 and 14.5 post coitum (p.c.). The degree of chimerism of donor cells in recipient newborn mice was examined using fluorescent microscopy, polymerase chain reaction (PCR), fluorescence-activated cell sorting (FACS), and fluorescence in situ hybridization (FISH) analyses. Microscopic examination revealed the presence of green fluorescent signal in the peripheral blood of the chimeric mice....
Source: Experimental and Molecular Pathology - August 6, 2009 Category: Pathology Authors: Chen X, Gong XL, Katsumata M, Zeng YT, Huang SZ, Zeng F Tags: Exp Mol Pathol Source Type: journals

Non-invasive Diagnosis of Early Pulmonary Disease in PECAM Deficient Mice Using Infrared Pulse Oximetry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pulse oximetry is a common tool for detecting reduced pulmonary function in human interstitial lung diseases. It has not previously been used in a mouse model of interstitial lung disease. Further, Platelet Endothelial Cell Adhesion Molecule deficient mice rarely show symptoms until disease is advanced. Using blood oxygen saturation, different stages of disease could be identified in a non-invasive manner. These stages could be correlated to pathology. Collagen deposition, using Picrosirius Red, did correlate with blood oxygen saturation. These studies are the first to show the use of an infrared pulse oximetry system ...
Source: Experimental and Molecular Pathology - July 27, 2009 Category: Pathology Authors: Early MA, Lishnevsky M, Gilchrist JM, Higgins DM, Orme IM, Muller WA, Gonzalez-Juarerro M, Schenkel AR Tags: Exp Mol Pathol Source Type: journals

Eotaxin/CCL11 expression by infiltrating macrophages in rat heart transplants during ongoing acute rejection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Eotaxin/CCL11 chemokine is expressed in different organs, including the heart, but its precise cellular origin in the heart is unknown. Eotaxin is associated with Th2-like responses and exerts its chemotactic effect through the chemokine receptor-3 (CCR3), which is also expressed on mast cells (MC). The aim of our study was to find the cellular origin of eotaxin in the heart, and to assess whether expression is changing during ongoing acute heart transplant rejection, indicating a correlation with mast cell infiltration which we observed in a previous study. In a model of ongoing acute heart transplant rejection in the...
Source: Experimental and Molecular Pathology - July 21, 2009 Category: Pathology Authors: Zweifel M, Mueller C, Schaffner T, Dahinden C, Matozan K, Driscoll R, Mohacsi P Tags: Exp Mol Pathol Source Type: journals

Decreased TLR4 gene expression in leukemic leukocyte populations.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study thus analyzed gene expression of TLR4 in groups of lymphocytic leukemia cases, myeloid leukemia cases, cases of myeloid leukemia in remission, and normal controls by real-time quantitative reverse transcription PCR (qRT-PCR). It was observed that TLR4 gene expression was indeed decreased to a statistically significant degree (P<0.05) in both the lymphocytic leukemic subset and myeloid leukemic subset when compared to normal controls. Thus, further study is warranted into determining whether this decreased TLR4 expression contributes to the pathogenesis of leukemic clone development through an associated depre...
Source: Experimental and Molecular Pathology - July 21, 2009 Category: Pathology Authors: Webb RN, Cruse JM, Lewis RE Tags: Exp Mol Pathol Source Type: journals

Higher Random Oligos Concentration Improves Reverse Transcription Yield of cDNA from Bioptic Tissues and Quantitative RT-PCR Reliability.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Real time quantitative reverse transcription -PCR (qRT-PCR) is the most sensitive technique for detection and quantification of mRNA targets. Reliable quantification of gene expression in formalin-fixed, paraffin-embedded tissues (FFPE), however, has been subjected to serious limitations so far, mainly due to the fragmentation of RNA transcripts. We tried to improve the sensitivity and reliability of mRNA quantification in FFPE by boosting the reverse transcription (RT) step, that is neglected in most of the protocol analysis, but that represents the first confounding event in a quantitative analysis. For this purpose,...
Source: Experimental and Molecular Pathology - July 16, 2009 Category: Pathology Authors: Nardon E, Donada M, Bonin S, Dotti I, Stanta G Tags: Exp Mol Pathol Source Type: journals

Early Activation of Fibrogenesis in Transplanted Kidneys. A study on serial renal biopsies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSION: Our results suggest that RAS and TGFbeta1-related fibrogenic loops are activated as early as 2 months after kidney transplantation. PMID: 19616542 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - July 15, 2009 Category: Pathology Authors: Del Prete D, Ceol M, Anglani F, Vianello D, Tiralongo E, Valente M, Graziotto R, Bonfante L, Scaparrotta G, Furian L, Rigotti P, Gambaro G, D'Angelo A Tags: Exp Mol Pathol Source Type: journals

Morphological changes and molecular expressions of hepatocellular carcinoma cells in three-dimensional culture model.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we investigated whether HAb18G/CD147 enhanced the HCC migration and invasion in three-dimensional (3D) culture model through affecting the key molecules and enzymes involved in the metastatic processes, such as focal adhesion kinase (FAK), matrix metalloproteinases (MMPs) and cytoskeleton proteins. We found that, compared with those in 2D cell culture model, the expression of HAb18G/CD147 was significantly increased in 3D cell culture model, together with a high production of MMPs (P<0.01), an enhanced expression and activation of FAK(P<0.01) and a changed distribution of F-actin. In addition, the expr...
Source: Experimental and Molecular Pathology - July 13, 2009 Category: Pathology Authors: Wu YM, Tang J, Zhao P, Chen ZN, Jiang JL Tags: Exp Mol Pathol Source Type: journals

Prevalence of genetic thrombophilic polymorphisms in the Sri Lankan population - implications for association study design and clinical genetic testing services.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We investigated the prevalence of genotypes/alleles of single nucleotide polymorphisms (SNP) and haplotypes defined by them in three genes in which variations are associated with venous thromboembolism in 80 Sinhalese, 80 Sri Lankan Tamils and 80 Moors in the Sri Lankan population and compared the SNP data with that of other populations in Southern India and haplotype data with that of HapMap populations. The genes and polymorphisms investigated were Methylenetetrahydrofolate reductase (MTHFR) - 677C>T (rs1801133), 1298A>C (rs1801131), 1317T>C, 1793G>A (rs2274976); Factor V (F5) - 1691G>A (rs6025) and 40...
Source: Experimental and Molecular Pathology - July 6, 2009 Category: Pathology Authors: Dissanayake VH, Weerasekera LY, Gammulla CG, Jayasekara RW Tags: Exp Mol Pathol Source Type: journals

The inflammatory component of Kaposi sarcoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19591823 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - July 6, 2009 Category: Pathology Authors: Pantanowitz L, Moses AV, Dezube BJ Tags: Exp Mol Pathol Source Type: journals

Deacetylase Inhibition Increases Regulatory T Cell Function and Decreases Incidence and Severity of Collagen-induced Arthritis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Collagen-induced arthritis (CIA) is an established mouse model of disease with hallmarks of clinical rheumatoid arthritis. Histone/protein deacetylase inhibitors (HDACi) are known to inhibit the pathogenesis of CIA and other models of autoimmune disease, although the mechanisms responsible are unclear. Regulatory T cell (Treg) function is defective in rheumatoid arthritis. FOXP3 proteins in Tregs are present in a dynamic protein complex containing histone acetyltransferase and HDAC enzymes, and FOXP3 itself is acetylated on lysine residues. We therefore investigated the effects of HDACi therapy on regulatory T cell fun...
Source: Experimental and Molecular Pathology - July 1, 2009 Category: Pathology Authors: Saouaf SJ, Li B, Zhang G, Shen Y, Furuuchi N, Hancock WW, Greene MI Tags: Exp Mol Pathol Source Type: journals

Evaluation of the Nanosphere Verigene((R)) System and the Verigene((R))F5 / F2 / MTHFR Nucleic Acid Tests.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The Verigene((R))F5 / F2 / MTHFR Nucleic Acid Tests for the factor V (1691G>A), factor II (20210G>A) and MTHFR (677C>T) genes was a robust method for SNP detection without the need for DNA amplification. The ease of use and performance of this system makes it suitable for the clinical laboratory setting. PMID: 19573527 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - June 28, 2009 Category: Pathology Authors: Lefferts JA, Jannetto P, Tsongalis GJ Tags: Exp Mol Pathol Source Type: journals

IL-17 induces myocardial fibrosis and enhances RANKL/OPG and MMP/TIMP signaling in isoproterenol-induced heart failure.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSION: Our study demonstrates the contribution of IL-17 to myocardial fibrosis in isoproterenol-induced HF. IL-17 can regulate the RANKL/OPG and MMP/TIMP systems in this model. RANKL/OPG system is one of intermediaries between IL-17 and MMP-1 in cardiac fibroblasts. As a harmful cytokine, anti-IL-17 treatment is a potential therapeutic strategy in HF. PMID: 19527710 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - June 11, 2009 Category: Pathology Authors: Feng W, Li W, Liu W, Wang F, Li Y, Yan W Tags: Exp Mol Pathol Source Type: journals